Rh6BG340700

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0017156
Physical Location & Seq
Reverse (-)
57302433 .. 57303424
992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG340700.1

Sequence Viewer

Length: 804 bp
ATGGATTCTACAAAAGCATTATATTTGCAGCCCTATTCTTCTGCATCACAAGGATTTGAAATAATTCCAATGGAACAGCATCAACATCATCATCATCATGCACCCCCGCCTCGACCTCCAGGTCCTCCTATTAAAGTGAACCTCAAAAGTAATTGTTTGAATTGTCAAGTGAAGCTGAAGAAAAAGTTGCAGAAGATGAGTGGGGTGAATGATGTGGACATAGTTCCAGAACAAGGTGGCACTGTAACAGTTTCAGGTAGCATAGACTCTAAGGTGCTCATGGAGGTGATCCGAAATAATTACAAAAAGGCCAAGTTACTTTGCCCCTTTGATCAGAAGGAGCCCTCTGAAAATACAGTTGGAAAAAAAATTTCGTGCTCTGATGATGAACATGATGATAGCGAGGACGACAAAGACCACAATTTTGCTGTAAACAATAGTAACAACGATGCCGGTCTAAGTGAACCGAAGAAAGAGAAGAAGACGAAGATGTGTGGTATAATGAGATGGTTTGGCAAGAAAAGTAACCCTATGGCCATGCGTCCACCTCCTCCCGGACCATCACCTGGGCCTCGGCCCCCCATGGGTTTTCCGACAAATGGATTTTTCCTTGGGCCGCCAAGGCCCGGATTTCGGCCGGGAATGCCATGGATGCCGCCAATTGGGCCACCACCGCCATATGGGTATTTAGGGAGGCCGCCATTACCCACGGACTACGGATATCCTTTCCCGAGGTCGCCGCCAAGATCGAACCCTATGGTACACTACAGCAGTTATAGGGACAATTATCATCATCCATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

267

Amino Acids

29.83

Weight (kDa)

9.22

Isoelectric Point (pI)

56.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04900
fragaria_vesca FvH4_2g23991
malus_domestica MD11G1290300.v1.1
prunus_persica Prupe.7G246000_v2.0.a1 Prupe.8G245300_v2.0.a1
rosa_laevigata RLG00000012095
rosa_multiflora Rmu_sc0002226.1_g000022
rosa_roxburghii Rroxscaffold_7G00175820
rosa_rugosa Rorug06G0221100
rosa_samantha Rh6AG333700 Rh6BG340700 Rh6CG346900 Rh6DG334100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 120
AccB7I CCANNNNNTGG 1 cut(s) 566
AciI CCGC 6 cut(s) 107, 617, 656, 674, 698, 740
AclWI GGATC 1 cut(s) 283
AcoI YGGCCR 2 cut(s) 534, 635
AcsI RAATTY 1 cut(s) 369
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 1 cut(s) 762
AfiI CCNNNNNNNGG 9 cut(s) 233, 554, 566, 584, 599, 626, 633, 662, 680
AgsI TTSAA 2 cut(s) 59, 160
AjnI CCWGG 2 cut(s) 118, 565
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AluBI AGCT 1 cut(s) 175
AluI AGCT 1 cut(s) 175
Alw21I GWGCWC 2 cut(s) 279, 380
AlwI GGATC 1 cut(s) 283
Ama87I CYCGRG 1 cut(s) 730
AoxI GGCC 9 cut(s) 309, 534, 569, 575, 614, 623, 635, 665, 695
ApeKI GCWGC 1 cut(s) 28
ApoI RAATTY 1 cut(s) 369
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
Asp700I GAANNNNTTC 1 cut(s) 63
AspS9I GGNCC 7 cut(s) 122, 557, 569, 576, 614, 624, 665
AsuC2I CCSGG 3 cut(s) 555, 627, 639
AsuHPI GGTGA 3 cut(s) 217, 298, 555
AvaI CYCGRG 1 cut(s) 730
AvaII GGWCC 2 cut(s) 122, 557
BalI TGGCCA 1 cut(s) 536
BanII GRGCYC 1 cut(s) 345
BbsI GAAGAC 1 cut(s) 488
Bbv12I GWGCWC 2 cut(s) 279, 380
BbvI GCAGC 1 cut(s) 40
BccI CCATC 2 cut(s) 501, 568
BciT130I CCWGG 2 cut(s) 120, 567
BclI TGATCA 1 cut(s) 331
BcnI CCSGG 3 cut(s) 555, 627, 639
BfaI CTAG 1 cut(s) 802
BfmI CTRYAG 1 cut(s) 766
BglI GCCNNNNNGGC 2 cut(s) 622, 664
BisI GCNGC 5 cut(s) 29, 617, 656, 698, 740
BlsI GCNGC 5 cut(s) 30, 618, 657, 699, 741
Bme1390I CCNGG 5 cut(s) 120, 555, 567, 627, 639
Bme18I GGWCC 2 cut(s) 122, 557
BmeT110I CYCGRG 1 cut(s) 730
BmgT120I GGNCC 7 cut(s) 122, 557, 569, 576, 614, 624, 665
BmiI GGNNCC 2 cut(s) 342, 578
BmrFI CCNGG 5 cut(s) 120, 555, 567, 627, 639
BmsI GCATC 4 cut(s) 53, 88, 439, 642
BpiI GAAGAC 1 cut(s) 488
BpmI CTGGAG 1 cut(s) 102
BpuMI CCSGG 3 cut(s) 555, 627, 639
BsaJI CCNNGG 8 cut(s) 566, 572, 582, 610, 620, 647, 708, 731
Bsc4I CCNNNNNNNGG 9 cut(s) 233, 554, 566, 584, 599, 626, 633, 662, 680
Bse118I RCCGGY 1 cut(s) 452
BseBI CCWGG 2 cut(s) 120, 567
BseDI CCNNGG 8 cut(s) 566, 572, 582, 610, 620, 647, 708, 731
BseGI GGATG 2 cut(s) 657, 793
BseLI CCNNNNNNNGG 9 cut(s) 233, 554, 566, 584, 599, 626, 633, 662, 680
BseRI GAGGAG 1 cut(s) 540
BseX3I CGGCCG 1 cut(s) 635
BseXI GCAGC 1 cut(s) 40
Bsh1285I CGRYCG 1 cut(s) 638
BshFI GGCC 9 cut(s) 311, 536, 571, 577, 616, 625, 637, 667, 697
BsiEI CGRYCG 1 cut(s) 638
BsiHKAI GWGCWC 2 cut(s) 279, 380
BsiHKCI CYCGRG 1 cut(s) 730
BsiSI CCGG 4 cut(s) 453, 555, 627, 638
BslFI GGGAC 1 cut(s) 794
BslI CCNNNNNNNGG 9 cut(s) 233, 554, 566, 584, 599, 626, 633, 662, 680
BsmFI GGGAC 1 cut(s) 794
BsmI GAATGC 1 cut(s) 648
BsnI GGCC 9 cut(s) 311, 536, 571, 577, 616, 625, 637, 667, 697
BsoBI CYCGRG 1 cut(s) 730
Bsp1286I GDGCHC 3 cut(s) 279, 345, 380
Bsp143I GATC 3 cut(s) 288, 331, 746
Bsp19I CCATGG 2 cut(s) 582, 647
BspACI CCGC 6 cut(s) 107, 617, 656, 674, 698, 740
BspANI GGCC 9 cut(s) 311, 536, 571, 577, 616, 625, 637, 667, 697
BspLI GGNNCC 2 cut(s) 342, 578
BspPI GGATC 1 cut(s) 283
BsrFI RCCGGY 1 cut(s) 452
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 8 cut(s) 566, 572, 582, 610, 620, 647, 708, 731
BssMI GATC 3 cut(s) 288, 331, 746
BssT1I CCWWGG 4 cut(s) 582, 610, 620, 647
Bst2UI CCWGG 2 cut(s) 120, 567
Bst4CI ACNGT 3 cut(s) 244, 250, 358
BstDEI CTNAG 2 cut(s) 270, 458
BstDSI CCRYGG 3 cut(s) 582, 647, 708
BstF5I GGATG 2 cut(s) 657, 793
BstKTI GATC 3 cut(s) 291, 334, 749
BstMBI GATC 3 cut(s) 288, 331, 746
BstMCI CGRYCG 1 cut(s) 638
BstMWI GCNNNNNNNGC 5 cut(s) 622, 643, 652, 664, 673
BstNI CCWGG 2 cut(s) 120, 567
BstSCI CCNGG 5 cut(s) 118, 553, 565, 625, 637
BstSFI CTRYAG 1 cut(s) 766
BstV1I GCAGC 1 cut(s) 40
BstV2I GAAGAC 1 cut(s) 488
BstZI CGGCCG 1 cut(s) 635
BsuRI GGCC 9 cut(s) 311, 536, 571, 577, 616, 625, 637, 667, 697
BtgI CCRYGG 3 cut(s) 582, 647, 708
BtsCI GGATG 2 cut(s) 657, 793
BtsIMutI CAGTG 1 cut(s) 240
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 7 cut(s) 122, 557, 569, 576, 614, 624, 665
CseI GACGC 1 cut(s) 530
Csp6I GTAC 1 cut(s) 761
CviAII CATG 6 cut(s) 98, 280, 392, 538, 583, 648
CviQI GTAC 1 cut(s) 761
DdeI CTNAG 2 cut(s) 270, 458
DpnI GATC 3 cut(s) 290, 333, 748
DpnII GATC 3 cut(s) 288, 331, 746
DrdI GACNNNNNNGTC 1 cut(s) 120
DseDI GACNNNNNNGTC 1 cut(s) 120
EaeI YGGCCR 2 cut(s) 534, 635
EagI CGGCCG 1 cut(s) 635
EclXI CGGCCG 1 cut(s) 635
Eco130I CCWWGG 4 cut(s) 582, 610, 620, 647
Eco24I GRGCYC 1 cut(s) 345
Eco32I GATATC 1 cut(s) 722
Eco47I GGWCC 2 cut(s) 122, 557
Eco52I CGGCCG 1 cut(s) 635
Eco57I CTGAAG 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 730
EcoO109I RGGNCCY 1 cut(s) 122
EcoRII CCWGG 2 cut(s) 118, 565
EcoRV GATATC 1 cut(s) 722
EcoT14I CCWWGG 4 cut(s) 582, 610, 620, 647
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 4 cut(s) 582, 610, 620, 647
FaeI CATG 6 cut(s) 101, 283, 395, 541, 586, 651
FaqI GGGAC 1 cut(s) 794
FatI CATG 6 cut(s) 97, 279, 391, 537, 582, 647
FauI CCCGC 1 cut(s) 114
FauNDI CATATG 1 cut(s) 679
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 5 cut(s) 29, 617, 656, 698, 740
FokI GGATG 2 cut(s) 664, 780
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 5 cut(s) 29, 617, 656, 698, 740
FspBI CTAG 1 cut(s) 802
GluI GCNGC 5 cut(s) 29, 617, 656, 698, 740
GsuI CTGGAG 1 cut(s) 102
HaeIII GGCC 9 cut(s) 311, 536, 571, 577, 616, 625, 637, 667, 697
HapII CCGG 4 cut(s) 453, 555, 627, 638
HgaI GACGC 1 cut(s) 530
Hin1II CATG 6 cut(s) 101, 283, 395, 541, 586, 651
HinfI GANTC 2 cut(s) 5, 266
HpaII CCGG 4 cut(s) 453, 555, 627, 638
HphI GGTGA 3 cut(s) 217, 298, 555
Hpy166II GTNNAC 6 cut(s) 139, 217, 433, 464, 545, 763
Hpy188I TCNGA 5 cut(s) 293, 336, 349, 382, 594
Hpy188III TCNNGA 2 cut(s) 227, 730
Hpy8I GTNNAC 6 cut(s) 139, 217, 433, 464, 545, 763
HpyAV CCTTC 1 cut(s) 331
HpyCH4III ACNGT 3 cut(s) 244, 250, 358
HpyCH4V TGCA 4 cut(s) 28, 44, 101, 190
HpyF10VI GCNNNNNNNGC 5 cut(s) 622, 643, 652, 664, 673
HpyF3I CTNAG 2 cut(s) 270, 458
Hsp92II CATG 6 cut(s) 101, 283, 395, 541, 586, 651
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 3 cut(s) 288, 331, 746
LmnI GCTCC 1 cut(s) 340
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 4 cut(s) 53, 88, 439, 642
MaeI CTAG 1 cut(s) 802
MaeIII GTNAC 4 cut(s) 244, 315, 440, 524
MalI GATC 3 cut(s) 290, 333, 748
MboI GATC 3 cut(s) 288, 331, 746
MboII GAAGA 7 cut(s) 30, 190, 205, 481, 490, 493, 499
MfeI CAATTG 1 cut(s) 660
MhlI GDGCHC 3 cut(s) 279, 345, 380
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 8 cut(s) 63, 151, 160, 298, 369, 421, 660, 784
MluNI TGGCCA 1 cut(s) 536
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 2 cut(s) 340, 617
Mox20I TGGCCA 1 cut(s) 536
MroXI GAANNNNTTC 1 cut(s) 63
MscI TGGCCA 1 cut(s) 536
MseI TTAA 1 cut(s) 132
MslI CAYNNNNRTG 2 cut(s) 96, 284
Msp20I TGGCCA 1 cut(s) 536
MspI CCGG 4 cut(s) 453, 555, 627, 638
MspR9I CCNGG 5 cut(s) 120, 555, 567, 627, 639
MunI CAATTG 1 cut(s) 660
Mva1269I GAATGC 1 cut(s) 648
MvaI CCWGG 2 cut(s) 120, 567
MwoI GCNNNNNNNGC 5 cut(s) 622, 643, 652, 664, 673
NciI CCSGG 3 cut(s) 555, 627, 639
NcoI CCATGG 2 cut(s) 582, 647
NdeI CATATG 1 cut(s) 679
NdeII GATC 3 cut(s) 288, 331, 746
NlaIII CATG 6 cut(s) 101, 283, 395, 541, 586, 651
NlaIV GGNNCC 2 cut(s) 342, 578
NmeAIII GCCGAG 1 cut(s) 553
PctI GAATGC 1 cut(s) 648
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 1 cut(s) 5
PflMI CCANNNNNTGG 1 cut(s) 566
PfoI TCCNGGA 1 cut(s) 553
PkrI GCNGC 5 cut(s) 30, 618, 657, 699, 741
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PpuMI RGGWCCY 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 2 cut(s) 118, 565
PspGI CCWGG 2 cut(s) 118, 565
PspN4I GGNNCC 2 cut(s) 342, 578
PspPI GGNCC 7 cut(s) 122, 557, 569, 576, 614, 624, 665
PspPPI RGGWCCY 1 cut(s) 122
RsaI GTAC 1 cut(s) 762
RsaNI GTAC 1 cut(s) 761
RseI CAYNNNNRTG 2 cut(s) 96, 284
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 5 cut(s) 29, 617, 656, 698, 740
Sau3AI GATC 3 cut(s) 288, 331, 746
Sau96I GGNCC 7 cut(s) 122, 557, 569, 576, 614, 624, 665
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 5 cut(s) 120, 555, 567, 627, 639
SduI GDGCHC 3 cut(s) 279, 345, 380
SfaNI GCATC 4 cut(s) 53, 88, 439, 642
SfcI CTRYAG 1 cut(s) 766
SfiI GGCCNNNNNGGCC 1 cut(s) 622
SinI GGWCC 2 cut(s) 122, 557
SmiMI CAYNNNNRTG 2 cut(s) 96, 284
Sse9I AATT 8 cut(s) 63, 151, 160, 298, 369, 421, 660, 784
SsiI CCGC 6 cut(s) 107, 617, 656, 674, 698, 740
SspMI CTAG 1 cut(s) 802
StyD4I CCNGG 5 cut(s) 118, 553, 565, 625, 637
StyI CCWWGG 4 cut(s) 582, 610, 620, 647
TaaI ACNGT 3 cut(s) 244, 250, 358
TaqI TCGA 2 cut(s) 112, 749
TasI AATT 8 cut(s) 63, 151, 160, 298, 369, 421, 660, 784
TauI GCSGC 4 cut(s) 619, 658, 700, 742
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TscAI CASTG 1 cut(s) 247
TseI GCWGC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 402
TspGWI ACGGA 2 cut(s) 725, 732
TspRI CASTG 1 cut(s) 247
Van91I CCANNNNNTGG 1 cut(s) 566
VpaK11BI GGWCC 2 cut(s) 122, 557
XapI RAATTY 1 cut(s) 369
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.