Prupe.8G245300_v2.0.a1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0017156
Physical Location & Seq
Reverse (-)
21322702 .. 21323880
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G245300.1

Sequence Viewer

Length: 729 bp
ATGGAGCAACATCGTGCACCCGTTCCGATTTGTACTATAAAAATGAATCTCCATTGTTGTGCAAAGTGTCCAATAAAACTGAAGGAAAAGTTGCAGAAACTCAATGGAGTGGAGGACATAAACATAGATCCAGAACAAGGATTATTAAAAGTTTCAGGTGACATAGACCCTATGGTTCTCACAAATATAGTCAAAAACATGGACAAAAAGGCAGAACTTTGGTCCTTCCAGAAAGAACCCTACAAAAACAATGTTAATATTGGTGCGTCTACTAAGTACAACATCCAAATTGGCGAAGATTGCTCTTGTGATGGTGATGAGGCTGAAAGCAGTTCGGATGATGAAAGCAAACGCAGTATTGCACCCAAGAAGCAACATGGAGTTCTCACTTGGGCCAAAAATGACAAGAAGAAGAAGAATGGGAAGGGATTTTCTGGCATGGGAATGGGCATGGGCATGCCTATGCCTCCTCAGCATCGGCCGCCTATGCGGCCATCTATGGGCATTCCGGCTCATAGGAGTTTTCATGGGATGCCCGGACTCGGATACTGGCCACCAAACCCATTTTATCAACCAATGGCGTATCATAGATCGTCACCGTCACCATATGGTTATTACGGACAAATTCAGCCACCACCTTATTCATATTTTCAATCAAGATCGCCACCCAAAGTTAACCCTATTGTCCACTACACTAACTATGCGGACAACTATCGGTACCCCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

243

Amino Acids

27.34

Weight (kDa)

9.14

Isoelectric Point (pI)

61.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04900
fragaria_vesca FvH4_2g23991
malus_domestica MD11G1290300.v1.1
prunus_persica Prupe.7G246000_v2.0.a1 Prupe.8G245300_v2.0.a1
rosa_laevigata RLG00000012095
rosa_multiflora Rmu_sc0002226.1_g000022
rosa_roxburghii Rroxscaffold_7G00175820
rosa_rugosa Rorug06G0221100
rosa_samantha Rh6AG333700 Rh6BG340700 Rh6CG346900 Rh6DG334100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 717
AccB1I GGYRCC 1 cut(s) 717
AccI GTMKAC 1 cut(s) 269
AciI CCGC 3 cut(s) 482, 490, 704
AclWI GGATC 1 cut(s) 122
AcoI YGGCCR 3 cut(s) 479, 491, 551
AcsI RAATTY 1 cut(s) 624
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 3 cut(s) 34, 278, 719
AfiI CCNNNNNNNGG 3 cut(s) 137, 500, 542
AgsI TTSAA 1 cut(s) 653
Alw21I GWGCWC 1 cut(s) 19
Alw44I GTGCAC 1 cut(s) 15
AlwI GGATC 1 cut(s) 122
AoxI GGCC 4 cut(s) 393, 479, 491, 551
ApaLI GTGCAC 1 cut(s) 15
ApoI RAATTY 1 cut(s) 624
Asp718I GGTACC 1 cut(s) 717
AspS9I GGNCC 2 cut(s) 222, 393
AsuC2I CCSGG 1 cut(s) 537
AsuHPI GGTGA 4 cut(s) 170, 326, 588, 594
AvaII GGWCC 1 cut(s) 222
BaeGI GKGCMC 1 cut(s) 19
BalI TGGCCA 1 cut(s) 553
BanI GGYRCC 1 cut(s) 717
Bbv12I GWGCWC 1 cut(s) 19
BbvCI CCTCAGC 1 cut(s) 471
BccI CCATC 2 cut(s) 305, 502
BciVI GTATCC 1 cut(s) 539
BcnI CCSGG 1 cut(s) 537
BfuI GTATCC 1 cut(s) 539
BglI GCCNNNNNGGC 1 cut(s) 490
BisI GCNGC 2 cut(s) 482, 491
BlsI GCNGC 2 cut(s) 483, 492
Bme1390I CCNGG 1 cut(s) 537
Bme18I GGWCC 1 cut(s) 222
BmgT120I GGNCC 2 cut(s) 222, 393
BmiI GGNNCC 1 cut(s) 719
BmrFI CCNGG 1 cut(s) 537
BmsI GCATC 2 cut(s) 484, 522
Bpu10I CCTNAGC 1 cut(s) 471
BpuMI CCSGG 1 cut(s) 537
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 500, 542
Bse1I ACTGG 1 cut(s) 554
BseGI GGATG 3 cut(s) 282, 343, 537
BseLI CCNNNNNNNGG 3 cut(s) 137, 500, 542
BseMII CTCAG 1 cut(s) 485
BseNI ACTGG 1 cut(s) 554
BseRI GAGGAG 1 cut(s) 459
BseSI GKGCMC 1 cut(s) 19
BseX3I CGGCCG 1 cut(s) 479
Bsh1285I CGRYCG 1 cut(s) 482
BshFI GGCC 4 cut(s) 395, 481, 493, 553
BshNI GGYRCC 1 cut(s) 717
BsiEI CGRYCG 1 cut(s) 482
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 2 cut(s) 509, 537
BslI CCNNNNNNNGG 3 cut(s) 137, 500, 542
BsmI GAATGC 1 cut(s) 504
BsnI GGCC 4 cut(s) 395, 481, 493, 553
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 3 cut(s) 127, 590, 659
BspACI CCGC 3 cut(s) 482, 490, 704
BspANI GGCC 4 cut(s) 395, 481, 493, 553
BspCNI CTCAG 1 cut(s) 484
BspLI GGNNCC 1 cut(s) 719
BspPI GGATC 1 cut(s) 122
BspT107I GGYRCC 1 cut(s) 717
BsrI ACTGG 1 cut(s) 554
BssMI GATC 3 cut(s) 127, 590, 659
Bst4CI ACNGT 1 cut(s) 600
BstC8I GCNNGC 1 cut(s) 458
BstDEI CTNAG 2 cut(s) 273, 471
BstF5I GGATG 3 cut(s) 282, 343, 537
BstKTI GATC 3 cut(s) 130, 593, 662
BstMBI GATC 3 cut(s) 127, 590, 659
BstMCI CGRYCG 1 cut(s) 482
BstMWI GCNNNNNNNGC 5 cut(s) 300, 472, 481, 487, 490
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 1 cut(s) 535
BstSLI GKGCMC 1 cut(s) 19
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
BstZI CGGCCG 1 cut(s) 479
BsuI GTATCC 1 cut(s) 539
BsuRI GGCC 4 cut(s) 395, 481, 493, 553
BtsCI GGATG 3 cut(s) 282, 343, 537
Cac8I GCNNGC 1 cut(s) 458
Cfr13I GGNCC 2 cut(s) 222, 393
CseI GACGC 1 cut(s) 255
Csp6I GTAC 3 cut(s) 33, 277, 718
CviAII CATG 7 cut(s) 199, 377, 439, 451, 457, 527, 724
CviJI RGCY 7 cut(s) 323, 395, 481, 493, 512, 553, 631
CviKI_1 RGCY 7 cut(s) 323, 395, 481, 493, 512, 553, 631
CviQI GTAC 3 cut(s) 33, 277, 718
DdeI CTNAG 2 cut(s) 273, 471
DpnI GATC 3 cut(s) 129, 592, 661
DpnII GATC 3 cut(s) 127, 590, 659
EaeI YGGCCR 3 cut(s) 479, 491, 551
EagI CGGCCG 1 cut(s) 479
EclXI CGGCCG 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 222
Eco52I CGGCCG 1 cut(s) 479
Eco57I CTGAAG 1 cut(s) 101
FaeI CATG 7 cut(s) 202, 380, 442, 454, 460, 530, 727
FatI CATG 7 cut(s) 198, 376, 438, 450, 456, 526, 723
FauNDI CATATG 1 cut(s) 607
FblI GTMKAC 1 cut(s) 269
Fnu4HI GCNGC 2 cut(s) 482, 491
FokI GGATG 3 cut(s) 269, 350, 544
Fsp4HI GCNGC 2 cut(s) 482, 491
GluI GCNGC 2 cut(s) 482, 491
HaeIII GGCC 4 cut(s) 395, 481, 493, 553
HapII CCGG 2 cut(s) 509, 537
HgaI GACGC 1 cut(s) 255
Hin1II CATG 7 cut(s) 202, 380, 442, 454, 460, 530, 727
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HinfI GANTC 2 cut(s) 46, 540
HpaI GTTAAC 1 cut(s) 676
HpaII CCGG 2 cut(s) 509, 537
HphI GGTGA 4 cut(s) 170, 326, 588, 594
Hpy166II GTNNAC 4 cut(s) 17, 270, 676, 688
Hpy188I TCNGA 3 cut(s) 27, 337, 545
Hpy188III TCNNGA 3 cut(s) 131, 229, 657
Hpy8I GTNNAC 4 cut(s) 17, 270, 676, 688
HpyAV CCTTC 3 cut(s) 76, 235, 418
HpyCH4III ACNGT 1 cut(s) 600
HpyCH4V TGCA 4 cut(s) 17, 62, 94, 362
HpyF10VI GCNNNNNNNGC 5 cut(s) 300, 472, 481, 487, 490
HpyF3I CTNAG 2 cut(s) 273, 471
Hsp92II CATG 7 cut(s) 202, 380, 442, 454, 460, 530, 727
KpnI GGTACC 1 cut(s) 721
KspAI GTTAAC 1 cut(s) 676
Kzo9I GATC 3 cut(s) 127, 590, 659
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 7 cut(s) 141, 144, 242, 420, 522, 535, 550
LweI GCATC 2 cut(s) 484, 522
MaeIII GTNAC 3 cut(s) 158, 594, 600
MalI GATC 3 cut(s) 129, 592, 661
MboI GATC 3 cut(s) 127, 590, 659
MboII GAAGA 4 cut(s) 308, 421, 424, 427
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 1 cut(s) 19
MlsI TGGCCA 1 cut(s) 553
MluCI AATT 2 cut(s) 288, 624
MluNI TGGCCA 1 cut(s) 553
MlyI GAGTC 1 cut(s) 534
MnlI CCTC 4 cut(s) 106, 313, 477, 480
Mox20I TGGCCA 1 cut(s) 553
MscI TGGCCA 1 cut(s) 553
MseI TTAA 3 cut(s) 146, 255, 675
MslI CAYNNNNRTG 4 cut(s) 57, 443, 455, 461
Msp20I TGGCCA 1 cut(s) 553
MspI CCGG 2 cut(s) 509, 537
MspR9I CCNGG 1 cut(s) 537
Mva1269I GAATGC 1 cut(s) 504
MwoI GCNNNNNNNGC 5 cut(s) 300, 472, 481, 487, 490
NciI CCSGG 1 cut(s) 537
NdeI CATATG 1 cut(s) 607
NdeII GATC 3 cut(s) 127, 590, 659
NlaIII CATG 7 cut(s) 202, 380, 442, 454, 460, 530, 727
NlaIV GGNNCC 1 cut(s) 719
NmuCI GTSAC 3 cut(s) 158, 594, 600
NspI RCATGY 1 cut(s) 460
PaeI GCATGC 1 cut(s) 460
PctI GAATGC 1 cut(s) 504
PfeI GAWTC 1 cut(s) 46
PkrI GCNGC 2 cut(s) 483, 492
PleI GAGTC 1 cut(s) 534
PpsI GAGTC 1 cut(s) 534
PspN4I GGNNCC 1 cut(s) 719
PspPI GGNCC 2 cut(s) 222, 393
PsuI RGATCY 1 cut(s) 127
RsaI GTAC 3 cut(s) 34, 278, 719
RsaNI GTAC 3 cut(s) 33, 277, 718
RseI CAYNNNNRTG 4 cut(s) 57, 443, 455, 461
SaqAI TTAA 3 cut(s) 146, 255, 675
SatI GCNGC 2 cut(s) 482, 491
Sau3AI GATC 3 cut(s) 127, 590, 659
Sau96I GGNCC 2 cut(s) 222, 393
SchI GAGTC 1 cut(s) 534
ScrFI CCNGG 1 cut(s) 537
SduI GDGCHC 1 cut(s) 19
SetI ASST 2 cut(s) 160, 640
SfaNI GCATC 2 cut(s) 484, 522
SinI GGWCC 1 cut(s) 222
SmiMI CAYNNNNRTG 4 cut(s) 57, 443, 455, 461
SphI GCATGC 1 cut(s) 460
Sse9I AATT 2 cut(s) 288, 624
SsiI CCGC 3 cut(s) 482, 490, 704
SspI AATATT 1 cut(s) 259
StyD4I CCNGG 1 cut(s) 535
TaaI ACNGT 1 cut(s) 600
TasI AATT 2 cut(s) 288, 624
TatI WGTACW 2 cut(s) 32, 276
TauI GCSGC 2 cut(s) 484, 493
TfiI GAWTC 1 cut(s) 46
Tru1I TTAA 3 cut(s) 146, 255, 675
Tru9I TTAA 3 cut(s) 146, 255, 675
TseFI GTSAC 3 cut(s) 158, 594, 600
Tsp45I GTSAC 3 cut(s) 158, 594, 600
TspDTI ATGAA 4 cut(s) 59, 357, 515, 633
TspGWI ACGGA 1 cut(s) 633
VneI GTGCAC 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 222
XapI RAATTY 1 cut(s) 624
XceI RCATGY 1 cut(s) 460
XmiI GTMKAC 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.