Rh6DG334100

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0017156
Physical Location & Seq
Reverse (-)
53899644 .. 53900868
1225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG334100.1

Sequence Viewer

Length: 663 bp
ATGAGTGGGGTGAATGATGTGGACATAGTTCCAGAACAAGGTGGCACTGTAACAGTTTCAGGTAGCATAGACTCTAAGGTGCTCATGGAGGTGATCCGAAATAATTACAAAAAGGCCAAGTTACTTTGCCCCTTTGATCAGAAGGAGCCCTCTGAAAATACAGTTGGAAAAAAATTTTCGTGCTCTGATGATGAACATGATGATAGCGAGGACGACAAAGACCACAATTTTGCTGTAAACAATAGTAACAACGATGCCGGTCTAAGTGAACCGAAGAAAGAGAAGAAGACGAAGATGTGTGGTATAATGAGATGGTTTGGCAAGAAAAGTAACCCTATGGCCATGCGTCCACCTCCTCCCGGACCATCACCTGGGCCTCGGCCCCCCATGGGTTTTCCGACAAATGGATTTTTCCTTGGGCCGCCAAGGCCCGGATTTCGGCCGGGAATGCCATGGATGCCGCCAATTGGGCCACCACCGCCATATGGGTATTTAGGGAGGCCGCCATTACCCACGGACTACGGATATCCTTTCCCTAGGTCGCCGCCAAGATCGAACCCTATGATGCCAGCTGGGTATAAGCTGGAAATCCAAAACCTCAACTCCATCTCCTACCACCGAATCTGGGTAGCCATAGAGAGGACGCTGTCTAGTCGGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

220

Amino Acids

24.38

Weight (kDa)

9.06

Isoelectric Point (pI)

59.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04900
fragaria_vesca FvH4_2g23991
malus_domestica MD11G1290300.v1.1
prunus_persica Prupe.7G246000_v2.0.a1 Prupe.8G245300_v2.0.a1
rosa_laevigata RLG00000012095
rosa_multiflora Rmu_sc0002226.1_g000022
rosa_roxburghii Rroxscaffold_7G00175820
rosa_rugosa Rorug06G0221100
rosa_samantha Rh6AG333700 Rh6BG340700 Rh6CG346900 Rh6DG334100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 371
AciI CCGC 5 cut(s) 422, 461, 479, 503, 545
AclWI GGATC 1 cut(s) 88
AcoI YGGCCR 2 cut(s) 339, 440
AcsI RAATTY 1 cut(s) 173
AjnI CCWGG 1 cut(s) 370
AjuI GAANNNNNNNTTGG 2 cut(s) 147, 179
AluBI AGCT 2 cut(s) 572, 583
AluI AGCT 2 cut(s) 572, 583
Alw21I GWGCWC 2 cut(s) 84, 185
AlwI GGATC 1 cut(s) 88
AoxI GGCC 9 cut(s) 114, 339, 374, 380, 419, 428, 440, 470, 500
ApoI RAATTY 1 cut(s) 173
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
AspA2I CCTAGG 1 cut(s) 536
AspS9I GGNCC 6 cut(s) 362, 374, 381, 419, 429, 470
AsuC2I CCSGG 3 cut(s) 360, 432, 444
AsuHPI GGTGA 3 cut(s) 22, 103, 360
AvaII GGWCC 1 cut(s) 362
AvrII CCTAGG 1 cut(s) 536
BalI TGGCCA 1 cut(s) 341
BanII GRGCYC 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 293
Bbv12I GWGCWC 2 cut(s) 84, 185
BccI CCATC 3 cut(s) 306, 373, 614
BciT130I CCWGG 1 cut(s) 372
BclI TGATCA 1 cut(s) 136
BcnI CCSGG 3 cut(s) 360, 432, 444
BfaI CTAG 2 cut(s) 537, 651
BglI GCCNNNNNGGC 2 cut(s) 427, 469
BisI GCNGC 4 cut(s) 422, 461, 503, 545
BlnI CCTAGG 1 cut(s) 536
BlsI GCNGC 4 cut(s) 423, 462, 504, 546
Bme1390I CCNGG 4 cut(s) 360, 372, 432, 444
Bme18I GGWCC 1 cut(s) 362
BmgT120I GGNCC 6 cut(s) 362, 374, 381, 419, 429, 470
BmiI GGNNCC 2 cut(s) 147, 383
BmrFI CCNGG 4 cut(s) 360, 372, 432, 444
BmsI GCATC 3 cut(s) 244, 447, 555
BpiI GAAGAC 1 cut(s) 293
BpuMI CCSGG 3 cut(s) 360, 432, 444
BsaJI CCNNGG 8 cut(s) 371, 377, 387, 415, 425, 452, 513, 536
BsaXI ACNNNNNCTCC 4 cut(s) 587, 593, 617, 623
Bse118I RCCGGY 1 cut(s) 257
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 8 cut(s) 371, 377, 387, 415, 425, 452, 513, 536
BseGI GGATG 1 cut(s) 462
BseRI GAGGAG 1 cut(s) 345
BseX3I CGGCCG 1 cut(s) 440
BseYI CCCAGC 1 cut(s) 572
Bsh1285I CGRYCG 1 cut(s) 443
BshFI GGCC 9 cut(s) 116, 341, 376, 382, 421, 430, 442, 472, 502
BsiEI CGRYCG 1 cut(s) 443
BsiHKAI GWGCWC 2 cut(s) 84, 185
BsiSI CCGG 4 cut(s) 258, 360, 432, 443
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 9 cut(s) 116, 341, 376, 382, 421, 430, 442, 472, 502
Bsp1286I GDGCHC 3 cut(s) 84, 150, 185
Bsp143I GATC 3 cut(s) 93, 136, 551
Bsp19I CCATGG 2 cut(s) 387, 452
BspACI CCGC 5 cut(s) 422, 461, 479, 503, 545
BspANI GGCC 9 cut(s) 116, 341, 376, 382, 421, 430, 442, 472, 502
BspLI GGNNCC 2 cut(s) 147, 383
BspPI GGATC 1 cut(s) 88
BsrFI RCCGGY 1 cut(s) 257
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 8 cut(s) 371, 377, 387, 415, 425, 452, 513, 536
BssMI GATC 3 cut(s) 93, 136, 551
BssT1I CCWWGG 5 cut(s) 387, 415, 425, 452, 536
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 3 cut(s) 49, 55, 163
BstC8I GCNNGC 1 cut(s) 570
BstDEI CTNAG 2 cut(s) 75, 263
BstDSI CCRYGG 3 cut(s) 387, 452, 513
BstF5I GGATG 1 cut(s) 462
BstKTI GATC 3 cut(s) 96, 139, 554
BstMBI GATC 3 cut(s) 93, 136, 551
BstMCI CGRYCG 1 cut(s) 443
BstMWI GCNNNNNNNGC 5 cut(s) 427, 448, 457, 469, 478
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 4 cut(s) 358, 370, 430, 442
BstV2I GAAGAC 1 cut(s) 293
BstZI CGGCCG 1 cut(s) 440
BsuRI GGCC 9 cut(s) 116, 341, 376, 382, 421, 430, 442, 472, 502
BtgI CCRYGG 3 cut(s) 387, 452, 513
BtsCI GGATG 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 45
Cac8I GCNNGC 1 cut(s) 570
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 6 cut(s) 362, 374, 381, 419, 429, 470
CseI GACGC 2 cut(s) 335, 652
CviAII CATG 5 cut(s) 85, 197, 343, 388, 453
DdeI CTNAG 2 cut(s) 75, 263
DpnI GATC 3 cut(s) 95, 138, 553
DpnII GATC 3 cut(s) 93, 136, 551
EaeI YGGCCR 2 cut(s) 339, 440
EagI CGGCCG 1 cut(s) 440
EclXI CGGCCG 1 cut(s) 440
Eco130I CCWWGG 5 cut(s) 387, 415, 425, 452, 536
Eco24I GRGCYC 1 cut(s) 150
Eco32I GATATC 1 cut(s) 527
Eco47I GGWCC 1 cut(s) 362
Eco52I CGGCCG 1 cut(s) 440
EcoRII CCWGG 1 cut(s) 370
EcoRV GATATC 1 cut(s) 527
EcoT14I CCWWGG 5 cut(s) 387, 415, 425, 452, 536
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 5 cut(s) 387, 415, 425, 452, 536
FaeI CATG 5 cut(s) 88, 200, 346, 391, 456
FatI CATG 5 cut(s) 84, 196, 342, 387, 452
FauNDI CATATG 1 cut(s) 484
FbaI TGATCA 1 cut(s) 136
Fnu4HI GCNGC 4 cut(s) 422, 461, 503, 545
FokI GGATG 1 cut(s) 469
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 4 cut(s) 422, 461, 503, 545
FspBI CTAG 2 cut(s) 537, 651
GluI GCNGC 4 cut(s) 422, 461, 503, 545
GsaI CCCAGC 1 cut(s) 576
HaeIII GGCC 9 cut(s) 116, 341, 376, 382, 421, 430, 442, 472, 502
HapII CCGG 4 cut(s) 258, 360, 432, 443
HgaI GACGC 2 cut(s) 335, 652
Hin1II CATG 5 cut(s) 88, 200, 346, 391, 456
HinfI GANTC 2 cut(s) 71, 621
HpaII CCGG 4 cut(s) 258, 360, 432, 443
HphI GGTGA 3 cut(s) 22, 103, 360
Hpy166II GTNNAC 4 cut(s) 22, 238, 269, 350
Hpy188I TCNGA 6 cut(s) 98, 141, 154, 187, 399, 662
Hpy188III TCNNGA 1 cut(s) 32
Hpy8I GTNNAC 4 cut(s) 22, 238, 269, 350
HpyAV CCTTC 1 cut(s) 136
HpyCH4III ACNGT 3 cut(s) 49, 55, 163
HpyF10VI GCNNNNNNNGC 5 cut(s) 427, 448, 457, 469, 478
HpyF3I CTNAG 2 cut(s) 75, 263
Hsp92II CATG 5 cut(s) 88, 200, 346, 391, 456
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 3 cut(s) 93, 136, 551
LmnI GCTCC 1 cut(s) 145
LweI GCATC 3 cut(s) 244, 447, 555
MaeI CTAG 2 cut(s) 537, 651
MaeIII GTNAC 4 cut(s) 49, 120, 245, 329
MalI GATC 3 cut(s) 95, 138, 553
MboI GATC 3 cut(s) 93, 136, 551
MboII GAAGA 4 cut(s) 286, 295, 298, 304
MfeI CAATTG 1 cut(s) 465
MhlI GDGCHC 3 cut(s) 84, 150, 185
MlsI TGGCCA 1 cut(s) 341
MluCI AATT 4 cut(s) 103, 173, 226, 465
MluNI TGGCCA 1 cut(s) 341
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 2 cut(s) 145, 422
MnlI CCTC 9 cut(s) 82, 160, 202, 363, 366, 387, 492, 608, 633
Mox20I TGGCCA 1 cut(s) 341
MscI TGGCCA 1 cut(s) 341
MslI CAYNNNNRTG 1 cut(s) 89
Msp20I TGGCCA 1 cut(s) 341
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 4 cut(s) 258, 360, 432, 443
MspR9I CCNGG 4 cut(s) 360, 372, 432, 444
MunI CAATTG 1 cut(s) 465
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 1 cut(s) 372
MwoI GCNNNNNNNGC 5 cut(s) 427, 448, 457, 469, 478
NciI CCSGG 3 cut(s) 360, 432, 444
NcoI CCATGG 2 cut(s) 387, 452
NdeI CATATG 1 cut(s) 484
NdeII GATC 3 cut(s) 93, 136, 551
NlaIII CATG 5 cut(s) 88, 200, 346, 391, 456
NlaIV GGNNCC 2 cut(s) 147, 383
NmeAIII GCCGAG 1 cut(s) 358
PctI GAATGC 1 cut(s) 453
PfeI GAWTC 1 cut(s) 621
PflFI GACNNNGTC 1 cut(s) 646
PflMI CCANNNNNTGG 1 cut(s) 371
PfoI TCCNGGA 1 cut(s) 358
PkrI GCNGC 4 cut(s) 423, 462, 504, 546
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
Psp6I CCWGG 1 cut(s) 370
PspFI CCCAGC 1 cut(s) 572
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 2 cut(s) 147, 383
PspPI GGNCC 6 cut(s) 362, 374, 381, 419, 429, 470
PsyI GACNNNGTC 1 cut(s) 646
PvuII CAGCTG 1 cut(s) 572
RseI CAYNNNNRTG 1 cut(s) 89
SatI GCNGC 4 cut(s) 422, 461, 503, 545
Sau3AI GATC 3 cut(s) 93, 136, 551
Sau96I GGNCC 6 cut(s) 362, 374, 381, 419, 429, 470
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 4 cut(s) 360, 372, 432, 444
SduI GDGCHC 3 cut(s) 84, 150, 185
SfaNI GCATC 3 cut(s) 244, 447, 555
SfiI GGCCNNNNNGGCC 1 cut(s) 427
SinI GGWCC 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 89
Sse9I AATT 4 cut(s) 103, 173, 226, 465
SsiI CCGC 5 cut(s) 422, 461, 479, 503, 545
SspMI CTAG 2 cut(s) 537, 651
StyD4I CCNGG 4 cut(s) 358, 370, 430, 442
StyI CCWWGG 5 cut(s) 387, 415, 425, 452, 536
TaaI ACNGT 3 cut(s) 49, 55, 163
TaqI TCGA 1 cut(s) 554
TasI AATT 4 cut(s) 103, 173, 226, 465
TauI GCSGC 4 cut(s) 424, 463, 505, 547
TfiI GAWTC 1 cut(s) 621
TscAI CASTG 1 cut(s) 52
TspDTI ATGAA 1 cut(s) 207
TspGWI ACGGA 2 cut(s) 530, 537
TspRI CASTG 1 cut(s) 52
Tth111I GACNNNGTC 1 cut(s) 646
Van91I CCANNNNNTGG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 362
XapI RAATTY 1 cut(s) 173
XmaJI CCTAGG 1 cut(s) 536
XspI CTAG 2 cut(s) 537, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.