MD11G1290300.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0017156
Physical Location & Seq
Reverse (-)
40995367 .. 40996350
984 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1290300.v1.1.491

Sequence Viewer

Length: 696 bp
ATGAAATATCTCCGATGTTGTGCTAAGTGTCCACTAAAAGTGAAAGAAAAGTTGCAGAAACTAGACGGAGTGGAAGATATAAACGTAGATCCAGAACAAGGGTTGCTAAGAGTTCAAGGTAAAATAGACCCTATGGTTCTCACAAGGGCAGTCCAAACTATGGACAAAAAGGCAGAACTTTGGTCCTTTCAGAAAGAACCCTTCCCAAGTAATATTGGTAAATCTTCTAAGTACAACTTCCAAATTGGCAAAGAGTGTTCATGCGAGGGCGGTTGTGATGATCATGAAAGCAGCTCCGATGATGAAAGCCCTAGTAGCAGTAAAGTTGTACCCAAGAAGCAATATGGTATTCTCAGTTGGATCAAAAAGGACAAGACGAAGAAGAATGGTAATAATAAGGCAACTTCTAGCACGGCCATGCCTATGCCTCGGCCGAGACCTGGACTTCGGCCGCCTATGGTTCCACCCATGGGCATTCCAGCAAATAGGAACTTTTATGGCCCTCCAAGGCACAGTTATTGGCCACTAAACCCATACTATCAACCTGTGGCGTACCATAGGCCACCACCGCCACCATATAGTTACTATGGACAAATGCAGCAGCCGCCCTATTCATATTTCCGGTCAAGGTCCCCACCGAAAGTTAACCCTGTCGTCCACTACACCAGTTATGCGGACAACTATCGTTATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

232

Amino Acids

26.4

Weight (kDa)

9.49

Isoelectric Point (pI)

60.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 7 - 55 5.4e-09 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04900
fragaria_vesca FvH4_2g23991
malus_domestica MD11G1290300.v1.1
prunus_persica Prupe.7G246000_v2.0.a1 Prupe.8G245300_v2.0.a1
rosa_laevigata RLG00000012095
rosa_multiflora Rmu_sc0002226.1_g000022
rosa_roxburghii Rroxscaffold_7G00175820
rosa_rugosa Rorug06G0221100
rosa_samantha Rh6AG333700 Rh6BG340700 Rh6CG346900 Rh6DG334100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 160
AciI CCGC 5 cut(s) 270, 452, 569, 605, 674
AclWI GGATC 2 cut(s) 83, 368
AcoI YGGCCR 4 cut(s) 414, 431, 449, 521
AfaI GTAC 3 cut(s) 233, 330, 554
AfiI CCNNNNNNNGG 4 cut(s) 98, 160, 440, 470
AgsI TTSAA 1 cut(s) 116
AjnI CCWGG 1 cut(s) 439
AluBI AGCT 1 cut(s) 294
AluI AGCT 1 cut(s) 294
Alw26I GTCTC 1 cut(s) 430
AlwI GGATC 2 cut(s) 83, 368
AoxI GGCC 6 cut(s) 414, 431, 449, 499, 521, 560
ApeKI GCWGC 3 cut(s) 291, 598, 601
ArsI GACNNNNNNTTYG 2 cut(s) 429, 461
AspS9I GGNCC 3 cut(s) 183, 500, 630
AvaII GGWCC 2 cut(s) 183, 630
BalI TGGCCA 1 cut(s) 523
BbvI GCAGC 3 cut(s) 303, 610, 613
BceAI ACGGC 1 cut(s) 429
BciT130I CCWGG 1 cut(s) 441
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 430
BfaI CTAG 3 cut(s) 62, 312, 408
BisI GCNGC 5 cut(s) 292, 452, 599, 602, 605
BlsI GCNGC 5 cut(s) 293, 453, 600, 603, 606
Bme1390I CCNGG 1 cut(s) 441
Bme18I GGWCC 2 cut(s) 183, 630
BmgT120I GGNCC 3 cut(s) 183, 500, 630
BmiI GGNNCC 2 cut(s) 462, 632
BmrFI CCNGG 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 430
BsaJI CCNNGG 3 cut(s) 428, 468, 506
BsaWI WCCGGW 1 cut(s) 621
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 160, 440, 470
Bse1I ACTGG 1 cut(s) 666
BseBI CCWGG 1 cut(s) 441
BseDI CCNNGG 3 cut(s) 428, 468, 506
BseLI CCNNNNNNNGG 4 cut(s) 98, 160, 440, 470
BseMII CTCAG 1 cut(s) 367
BseNI ACTGG 1 cut(s) 666
BseX3I CGGCCG 2 cut(s) 431, 449
BseXI GCAGC 3 cut(s) 303, 610, 613
Bsh1285I CGRYCG 2 cut(s) 434, 452
BshFI GGCC 6 cut(s) 416, 433, 451, 501, 523, 562
BsiEI CGRYCG 2 cut(s) 434, 452
BsiSI CCGG 1 cut(s) 622
BslFI GGGAC 1 cut(s) 616
BslI CCNNNNNNNGG 4 cut(s) 98, 160, 440, 470
BsmAI GTCTC 1 cut(s) 430
BsmFI GGGAC 1 cut(s) 616
BsmI GAATGC 1 cut(s) 474
BsnI GGCC 6 cut(s) 416, 433, 451, 501, 523, 562
Bso31I GGTCTC 1 cut(s) 430
Bsp143I GATC 3 cut(s) 88, 280, 360
Bsp19I CCATGG 1 cut(s) 468
BspACI CCGC 5 cut(s) 270, 452, 569, 605, 674
BspANI GGCC 6 cut(s) 416, 433, 451, 501, 523, 562
BspCNI CTCAG 1 cut(s) 366
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 2 cut(s) 462, 632
BspPI GGATC 2 cut(s) 83, 368
BspTNI GGTCTC 1 cut(s) 430
BsrI ACTGG 1 cut(s) 666
BssECI CCNNGG 3 cut(s) 428, 468, 506
BssMI GATC 3 cut(s) 88, 280, 360
BssT1I CCWWGG 2 cut(s) 468, 506
Bst2UI CCWGG 1 cut(s) 441
Bst4CI ACNGT 1 cut(s) 515
BstDEI CTNAG 4 cut(s) 24, 107, 228, 353
BstDSI CCRYGG 1 cut(s) 468
BstKTI GATC 3 cut(s) 91, 283, 363
BstMAI GTCTC 1 cut(s) 430
BstMBI GATC 3 cut(s) 88, 280, 360
BstMCI CGRYCG 2 cut(s) 434, 452
BstMWI GCNNNNNNNGC 3 cut(s) 315, 568, 604
BstNI CCWGG 1 cut(s) 441
BstSCI CCNGG 1 cut(s) 439
BstV1I GCAGC 3 cut(s) 303, 610, 613
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
BstZI CGGCCG 2 cut(s) 431, 449
BsuRI GGCC 6 cut(s) 416, 433, 451, 501, 523, 562
BtgI CCRYGG 1 cut(s) 468
CciI TCATGA 1 cut(s) 283
Cfr13I GGNCC 3 cut(s) 183, 500, 630
Csp6I GTAC 3 cut(s) 232, 329, 553
CviAII CATG 5 cut(s) 261, 284, 418, 469, 693
CviJI RGCY 9 cut(s) 294, 309, 416, 433, 451, 501, 523, 562, 604
CviKI_1 RGCY 9 cut(s) 294, 309, 416, 433, 451, 501, 523, 562, 604
CviQI GTAC 3 cut(s) 232, 329, 553
DdeI CTNAG 4 cut(s) 24, 107, 228, 353
DpnI GATC 3 cut(s) 90, 282, 362
DpnII GATC 3 cut(s) 88, 280, 360
EaeI YGGCCR 4 cut(s) 414, 431, 449, 521
EagI CGGCCG 2 cut(s) 431, 449
EclXI CGGCCG 2 cut(s) 431, 449
Eco130I CCWWGG 2 cut(s) 468, 506
Eco31I GGTCTC 1 cut(s) 430
Eco47I GGWCC 2 cut(s) 183, 630
Eco52I CGGCCG 2 cut(s) 431, 449
EcoO109I RGGNCCY 1 cut(s) 630
EcoRII CCWGG 1 cut(s) 439
EcoT14I CCWWGG 2 cut(s) 468, 506
ErhI CCWWGG 2 cut(s) 468, 506
FaeI CATG 5 cut(s) 264, 287, 421, 472, 696
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FaqI GGGAC 1 cut(s) 616
FatI CATG 5 cut(s) 260, 283, 417, 468, 692
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 5 cut(s) 292, 452, 599, 602, 605
Fsp4HI GCNGC 5 cut(s) 292, 452, 599, 602, 605
FspBI CTAG 3 cut(s) 62, 312, 408
GluI GCNGC 5 cut(s) 292, 452, 599, 602, 605
HaeIII GGCC 6 cut(s) 416, 433, 451, 501, 523, 562
HapII CCGG 1 cut(s) 622
Hin1II CATG 5 cut(s) 264, 287, 421, 472, 696
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HpaI GTTAAC 1 cut(s) 646
HpaII CCGG 1 cut(s) 622
Hpy166II GTNNAC 3 cut(s) 32, 646, 658
Hpy188I TCNGA 3 cut(s) 14, 192, 298
Hpy188III TCNNGA 2 cut(s) 92, 284
Hpy8I GTNNAC 3 cut(s) 32, 646, 658
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 1 cut(s) 515
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 2 cut(s) 55, 598
HpyF10VI GCNNNNNNNGC 3 cut(s) 315, 568, 604
HpyF3I CTNAG 4 cut(s) 24, 107, 228, 353
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 5 cut(s) 264, 287, 421, 472, 696
Ksp22I TGATCA 1 cut(s) 280
KspAI GTTAAC 1 cut(s) 646
Kzo9I GATC 3 cut(s) 88, 280, 360
LmnI GCTCC 1 cut(s) 299
LpnPI CCDG 8 cut(s) 105, 426, 453, 492, 558, 635, 663, 679
Lsp1109I GCAGC 3 cut(s) 303, 610, 613
MaeI CTAG 3 cut(s) 62, 312, 408
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 581
MalI GATC 3 cut(s) 90, 282, 362
MboI GATC 3 cut(s) 88, 280, 360
MboII GAAGA 4 cut(s) 86, 216, 391, 394
MflI RGATCY 1 cut(s) 88
MlsI TGGCCA 1 cut(s) 523
MluCI AATT 1 cut(s) 243
MluNI TGGCCA 1 cut(s) 523
MmeI TCCRAC 1 cut(s) 338
MnlI CCTC 3 cut(s) 259, 438, 513
Mox20I TGGCCA 1 cut(s) 523
MscI TGGCCA 1 cut(s) 523
MseI TTAA 1 cut(s) 645
MslI CAYNNNNRTG 2 cut(s) 416, 422
Msp20I TGGCCA 1 cut(s) 523
MspI CCGG 1 cut(s) 622
MspR9I CCNGG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 474
MvaI CCWGG 1 cut(s) 441
MwoI GCNNNNNNNGC 3 cut(s) 315, 568, 604
NcoI CCATGG 1 cut(s) 468
NdeII GATC 3 cut(s) 88, 280, 360
NlaIII CATG 5 cut(s) 264, 287, 421, 472, 696
NlaIV GGNNCC 2 cut(s) 462, 632
NmeAIII GCCGAG 2 cut(s) 409, 459
PagI TCATGA 1 cut(s) 283
PctI GAATGC 1 cut(s) 474
PflMI CCANNNNNTGG 1 cut(s) 160
PkrI GCNGC 5 cut(s) 293, 453, 600, 603, 606
PpuMI RGGWCCY 1 cut(s) 630
Psp5II RGGWCCY 1 cut(s) 630
Psp6I CCWGG 1 cut(s) 439
PspGI CCWGG 1 cut(s) 439
PspN4I GGNNCC 2 cut(s) 462, 632
PspPI GGNCC 3 cut(s) 183, 500, 630
PspPPI RGGWCCY 1 cut(s) 630
PsuI RGATCY 1 cut(s) 88
RsaI GTAC 3 cut(s) 233, 330, 554
RsaNI GTAC 3 cut(s) 232, 329, 553
RseI CAYNNNNRTG 2 cut(s) 416, 422
SaqAI TTAA 1 cut(s) 645
SatI GCNGC 5 cut(s) 292, 452, 599, 602, 605
Sau3AI GATC 3 cut(s) 88, 280, 360
Sau96I GGNCC 3 cut(s) 183, 500, 630
ScrFI CCNGG 1 cut(s) 441
SetI ASST 6 cut(s) 87, 121, 296, 442, 547, 632
SinI GGWCC 2 cut(s) 183, 630
SmiMI CAYNNNNRTG 2 cut(s) 416, 422
Sse9I AATT 1 cut(s) 243
SsiI CCGC 5 cut(s) 270, 452, 569, 605, 674
SspI AATATT 1 cut(s) 214
SspMI CTAG 3 cut(s) 62, 312, 408
StyD4I CCNGG 1 cut(s) 439
StyI CCWWGG 2 cut(s) 468, 506
TaaI ACNGT 1 cut(s) 515
TaiI ACGT 1 cut(s) 87
TasI AATT 1 cut(s) 243
TatI WGTACW 1 cut(s) 231
TauI GCSGC 2 cut(s) 454, 607
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TseI GCWGC 3 cut(s) 291, 598, 601
TspDTI ATGAA 5 cut(s) 17, 249, 300, 318, 603
TspGWI ACGGA 1 cut(s) 81
Van91I CCANNNNNTGG 1 cut(s) 160
VpaK11BI GGWCC 2 cut(s) 183, 630
XspI CTAG 3 cut(s) 62, 312, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.