Rorug06G0221100

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0017156
Physical Location & Seq
Forward (+)
35397052 .. 35397540
489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0221100.1

Sequence Viewer

Length: 465 bp
ATGACAGATCCCATCTCCAGCCTAGTGGCTGCTTTTGCTCTCAACAACAATGCTCATGTCGATCTCGGTTTGGCCGAGGAAGCGGTGCTGCAGGACAACCACCATTACATGGTGGGACAACTACTCACACGGAGGCCAAATCTGACTGGCTTCATTGGCACGATGCCCACTATATGGCGAATGAAGGGTGGTTTGTCCATTAGAGCGTTTGATCTTGAGGAAGATCGCAACCGTTATCTTCACGGAGGTCCGTGGTTCTACCGCAATACCATGCTTCTCTTGGCTCTGTATGATCGGATTGCTAATGTGGATTCAATCCCGCTTCATTCTTTGGAGCTTTGGATCACTGTTTGGGGGCTCCCTCCTACGTTGCGCAATCACCTTTCTCTCTCTATGATTGGATCTGCCATTGGTAGGGTTTTGAGGCTTGATACGGTGCCTTTGAAGCAGAAGGATGAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

154

Amino Acids

17.43

Weight (kDa)

6.29

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 31 - 95 1.9e-06 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04900
fragaria_vesca FvH4_2g23991
malus_domestica MD11G1290300.v1.1
prunus_persica Prupe.7G246000_v2.0.a1 Prupe.8G245300_v2.0.a1
rosa_laevigata RLG00000012095
rosa_multiflora Rmu_sc0002226.1_g000022
rosa_roxburghii Rroxscaffold_7G00175820
rosa_rugosa Rorug06G0221100
rosa_samantha Rh6AG333700 Rh6BG340700 Rh6CG346900 Rh6DG334100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 374
AccB1I GGYRCC 1 cut(s) 436
AccB7I CCANNNNNTGG 2 cut(s) 109, 174
AciI CCGC 3 cut(s) 83, 262, 320
AclWI GGATC 3 cut(s) 2, 350, 409
AcoI YGGCCR 1 cut(s) 72
AfiI CCNNNNNNNGG 3 cut(s) 109, 174, 414
AgsI TTSAA 2 cut(s) 315, 445
AluBI AGCT 1 cut(s) 337
AluI AGCT 1 cut(s) 337
AlwI GGATC 3 cut(s) 2, 350, 409
AoxI GGCC 2 cut(s) 72, 134
ApeKI GCWGC 2 cut(s) 29, 88
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 371
AvaII GGWCC 1 cut(s) 248
BanI GGYRCC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 360
BbvI GCAGC 2 cut(s) 16, 75
BccI CCATC 1 cut(s) 20
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 89
BisI GCNGC 2 cut(s) 30, 89
BlsI GCNGC 2 cut(s) 31, 90
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 2 cut(s) 359, 438
BmsI GCATC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 236
BsaJI CCNNGG 2 cut(s) 75, 251
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 174, 414
Bse1I ACTGG 1 cut(s) 151
BseDI CCNNGG 2 cut(s) 75, 251
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 3 cut(s) 109, 174, 414
BseNI ACTGG 1 cut(s) 151
BseXI GCAGC 2 cut(s) 16, 75
BshFI GGCC 2 cut(s) 74, 136
BshNI GGYRCC 1 cut(s) 436
BslFI GGGAC 1 cut(s) 129
BslI CCNNNNNNNGG 3 cut(s) 109, 174, 414
BsmFI GGGAC 1 cut(s) 129
BsnI GGCC 2 cut(s) 74, 136
Bsp1286I GDGCHC 1 cut(s) 360
Bsp143I GATC 7 cut(s) 7, 61, 211, 223, 292, 342, 401
BspACI CCGC 3 cut(s) 83, 262, 320
BspANI GGCC 2 cut(s) 74, 136
BspLI GGNNCC 2 cut(s) 359, 438
BspMAI CTGCAG 1 cut(s) 93
BspPI GGATC 3 cut(s) 2, 350, 409
BspT107I GGYRCC 1 cut(s) 436
BsrI ACTGG 1 cut(s) 151
BssECI CCNNGG 2 cut(s) 75, 251
BssMI GATC 7 cut(s) 7, 61, 211, 223, 292, 342, 401
Bst4CI ACNGT 3 cut(s) 233, 349, 436
BstDSI CCRYGG 1 cut(s) 251
BstF5I GGATG 1 cut(s) 460
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 7 cut(s) 10, 64, 214, 226, 295, 345, 404
BstMBI GATC 7 cut(s) 7, 61, 211, 223, 292, 342, 401
BstMWI GCNNNNNNNGC 4 cut(s) 35, 80, 156, 445
BstSFI CTRYAG 1 cut(s) 89
BstV1I GCAGC 2 cut(s) 16, 75
BstX2I RGATCY 2 cut(s) 7, 401
BstXI CCANNNNNNTGG 1 cut(s) 25
BstYI RGATCY 2 cut(s) 7, 401
BsuRI GGCC 2 cut(s) 74, 136
BtgI CCRYGG 1 cut(s) 251
BtsCI GGATG 1 cut(s) 460
BtsIMutI CAGTG 1 cut(s) 345
CfoI GCGC 1 cut(s) 375
Cfr13I GGNCC 1 cut(s) 248
CviAII CATG 3 cut(s) 56, 109, 271
CviJI RGCY 9 cut(s) 21, 29, 74, 136, 150, 284, 337, 358, 427
CviKI_1 RGCY 9 cut(s) 21, 29, 74, 136, 150, 284, 337, 358, 427
DpnI GATC 7 cut(s) 9, 63, 213, 225, 294, 344, 403
DpnII GATC 7 cut(s) 7, 61, 211, 223, 292, 342, 401
EaeI YGGCCR 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 360
Eco47I GGWCC 1 cut(s) 248
EcoT38I GRGCYC 1 cut(s) 360
FaeI CATG 3 cut(s) 59, 112, 274
FaiI YATR 7 cut(s) 57, 110, 173, 175, 272, 291, 395
FaqI GGGAC 1 cut(s) 129
FatI CATG 3 cut(s) 55, 108, 270
FauI CCCGC 1 cut(s) 327
Fnu4HI GCNGC 2 cut(s) 30, 89
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 2 cut(s) 30, 89
FspBI CTAG 1 cut(s) 23
FspI TGCGCA 1 cut(s) 374
GlaI GCGC 1 cut(s) 374
GluI GCNGC 2 cut(s) 30, 89
HaeIII GGCC 2 cut(s) 74, 136
HhaI GCGC 1 cut(s) 375
Hin1II CATG 3 cut(s) 59, 112, 274
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HinfI GANTC 1 cut(s) 311
HphI GGTGA 1 cut(s) 371
Hpy188I TCNGA 2 cut(s) 144, 297
Hpy188III TCNNGA 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 178, 445
HpyCH4III ACNGT 3 cut(s) 233, 349, 436
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 1 cut(s) 91
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 80, 156, 445
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 3 cut(s) 59, 112, 274
HspAI GCGC 1 cut(s) 373
Kzo9I GATC 7 cut(s) 7, 61, 211, 223, 292, 342, 401
LmnI GCTCC 2 cut(s) 334, 363
LpnPI CCDG 3 cut(s) 31, 77, 132
Lsp1109I GCAGC 2 cut(s) 16, 75
LweI GCATC 1 cut(s) 153
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 368
MalI GATC 7 cut(s) 9, 63, 213, 225, 294, 344, 403
MboI GATC 7 cut(s) 7, 61, 211, 223, 292, 342, 401
MboII GAAGA 2 cut(s) 230, 233
MflI RGATCY 2 cut(s) 7, 401
MhlI GDGCHC 1 cut(s) 360
MnlI CCTC 7 cut(s) 70, 126, 211, 239, 372, 417, 451
MwoI GCNNNNNNNGC 4 cut(s) 35, 80, 156, 445
NdeII GATC 7 cut(s) 7, 61, 211, 223, 292, 342, 401
NlaIII CATG 3 cut(s) 59, 112, 274
NlaIV GGNNCC 2 cut(s) 359, 438
NmeAIII GCCGAG 1 cut(s) 100
NsbI TGCGCA 1 cut(s) 374
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PfeI GAWTC 1 cut(s) 311
PflMI CCANNNNNTGG 2 cut(s) 109, 174
PkrI GCNGC 2 cut(s) 31, 90
PspN4I GGNNCC 2 cut(s) 359, 438
PspPI GGNCC 1 cut(s) 248
PstI CTGCAG 1 cut(s) 93
PsuI RGATCY 2 cut(s) 7, 401
SatI GCNGC 2 cut(s) 30, 89
Sau3AI GATC 7 cut(s) 7, 61, 211, 223, 292, 342, 401
Sau96I GGNCC 1 cut(s) 248
SduI GDGCHC 1 cut(s) 360
SetI ASST 4 cut(s) 250, 339, 371, 384
SfaNI GCATC 1 cut(s) 153
SfcI CTRYAG 1 cut(s) 89
SinI GGWCC 1 cut(s) 248
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SsiI CCGC 3 cut(s) 83, 262, 320
SspMI CTAG 1 cut(s) 23
TaaI ACNGT 3 cut(s) 233, 349, 436
TaiI ACGT 1 cut(s) 371
TaqI TCGA 1 cut(s) 60
TfiI GAWTC 1 cut(s) 311
TscAI CASTG 1 cut(s) 352
TseI GCWGC 2 cut(s) 29, 88
TspDTI ATGAA 3 cut(s) 142, 197, 314
TspGWI ACGGA 3 cut(s) 145, 240, 258
TspRI CASTG 1 cut(s) 352
Van91I CCANNNNNTGG 2 cut(s) 109, 174
VpaK11BI GGWCC 1 cut(s) 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 277
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.