RLG00000012095

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017156
Physical Location & Seq
Forward (+)
15211515 .. 15212518
1004 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012095

Sequence Viewer

Length: 813 bp
ATGGATTCTACAAAAGCATTATATTTGCAGCCCTATTCTTCTGCGTCACAAGGATTTGAAATAATTCCAATGGAACAGCATCAACATCAACATCATCATCATCATCATGCACCCCCGCCTCGACCTCCAGGTCCTCCTAGTAAAGTGAACCTCAAAAGTAATTGTTTGAATTGTCAAGTGAGGCTGAAGAAAAAGTTGCTGAAGATGAGTGGGGTGAATGATGTGGACATAGTTCCAGAACAAGGTGGCACTGTAACAGTTTCAGGTAGCATAGACTCTAAGGTGCTCATGGAGGTGATCCGAAATAATTACAAAAAGGCCAAGTTACTTTGCCCCTTCGATCAGAAGGAGTCCTCCGAAAATACAGTTGGAAAAAAATTTTCGTGCTCTGATGATGAACATGATGATAGCGAGGACGACAAAGACCACAATTTTGCTGTAAACAATAGTAACAACGATGCCGGTCTAAGTGAACCCAAGAAAGAGAAGAAGACGAAGATGTGTGGTATAATGAGATGTTTTGGCAAGAAAAGTAACCCTATGGCCATGCGTCCACCTCCTCCGGGACCAGCACCTGGGCCTCGGCCTCCCATGGGTTTTCCGACTAATGGATTTTTCCTTGGGCCGCCAAGGCCTGGATTTCGGCCGGGGATGCCATGGATGCCGCCAATTGGGCCACCACCGCCATATGGGTATTTAGGGAGGCCGCCGTTACCCACGGACTACGGATATCCTTTCCCGAGGTCGCCGCCAAGATCGAACCCTATGGTACACTACACCAGCTATATGGACAATTATCGTCATCCATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

271

Amino Acids

30.15

Weight (kDa)

9.18

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04900
fragaria_vesca FvH4_2g23991
malus_domestica MD11G1290300.v1.1
prunus_persica Prupe.7G246000_v2.0.a1 Prupe.8G245300_v2.0.a1
rosa_laevigata RLG00000012095
rosa_multiflora Rmu_sc0002226.1_g000022
rosa_roxburghii Rroxscaffold_7G00175820
rosa_rugosa Rorug06G0221100
rosa_samantha Rh6AG333700 Rh6BG340700 Rh6CG346900 Rh6DG334100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 129
AccB7I CCANNNNNTGG 2 cut(s) 575, 635
AciI CCGC 6 cut(s) 116, 626, 665, 683, 707, 749
AclWI GGATC 1 cut(s) 292
AcoI YGGCCR 2 cut(s) 543, 644
AcsI RAATTY 1 cut(s) 377
AcuI CTGAAG 2 cut(s) 206, 221
AfaI GTAC 1 cut(s) 771
AfiI CCNNNNNNNGG 8 cut(s) 242, 563, 575, 593, 608, 635, 671, 689
AgsI TTSAA 2 cut(s) 59, 169
AjnI CCWGG 3 cut(s) 127, 574, 634
AjuI GAANNNNNNNTTGG 2 cut(s) 351, 383
AluBI AGCT 1 cut(s) 783
AluI AGCT 1 cut(s) 783
Alw21I GWGCWC 2 cut(s) 288, 389
AlwI GGATC 1 cut(s) 292
AlwNI CAGNNNCTG 1 cut(s) 575
Ama87I CYCGRG 1 cut(s) 739
AoxI GGCC 9 cut(s) 318, 543, 578, 584, 623, 632, 644, 674, 704
ApeKI GCWGC 1 cut(s) 28
ApoI RAATTY 1 cut(s) 377
ArsI GACNNNNNNTTYG 2 cut(s) 416, 448
Asp700I GAANNNNTTC 1 cut(s) 63
AspS9I GGNCC 5 cut(s) 131, 566, 578, 623, 674
AsuC2I CCSGG 2 cut(s) 564, 648
AsuHPI GGTGA 2 cut(s) 226, 307
AvaI CYCGRG 1 cut(s) 739
AvaII GGWCC 2 cut(s) 131, 566
BalI TGGCCA 1 cut(s) 545
BbsI GAAGAC 1 cut(s) 497
Bbv12I GWGCWC 2 cut(s) 288, 389
BbvI GCAGC 1 cut(s) 40
BceAI ACGGC 1 cut(s) 694
BciT130I CCWGG 3 cut(s) 129, 576, 636
BcnI CCSGG 2 cut(s) 564, 648
BfaI CTAG 2 cut(s) 138, 811
BglI GCCNNNNNGGC 2 cut(s) 631, 673
BisI GCNGC 5 cut(s) 29, 626, 665, 707, 749
BlsI GCNGC 5 cut(s) 30, 627, 666, 708, 750
Bme1390I CCNGG 5 cut(s) 129, 564, 576, 636, 648
Bme18I GGWCC 2 cut(s) 131, 566
BmeT110I CYCGRG 1 cut(s) 739
BmgT120I GGNCC 5 cut(s) 131, 566, 578, 623, 674
BmiI GGNNCC 1 cut(s) 567
BmrFI CCNGG 5 cut(s) 129, 564, 576, 636, 648
BmsI GCATC 4 cut(s) 88, 448, 642, 651
BpiI GAAGAC 1 cut(s) 497
BpmI CTGGAG 1 cut(s) 111
BpuMI CCSGG 2 cut(s) 564, 648
BsaJI CCNNGG 9 cut(s) 575, 581, 591, 619, 629, 647, 656, 717, 740
Bsc4I CCNNNNNNNGG 8 cut(s) 242, 563, 575, 593, 608, 635, 671, 689
Bse118I RCCGGY 1 cut(s) 461
BseBI CCWGG 3 cut(s) 129, 576, 636
BseDI CCNNGG 9 cut(s) 575, 581, 591, 619, 629, 647, 656, 717, 740
BseGI GGATG 3 cut(s) 657, 666, 802
BseLI CCNNNNNNNGG 8 cut(s) 242, 563, 575, 593, 608, 635, 671, 689
BseRI GAGGAG 1 cut(s) 549
BseX3I CGGCCG 1 cut(s) 644
BseXI GCAGC 1 cut(s) 40
Bsh1285I CGRYCG 1 cut(s) 647
BshFI GGCC 9 cut(s) 320, 545, 580, 586, 625, 634, 646, 676, 706
BsiEI CGRYCG 1 cut(s) 647
BsiHKAI GWGCWC 2 cut(s) 288, 389
BsiHKCI CYCGRG 1 cut(s) 739
BsiSI CCGG 3 cut(s) 462, 563, 647
BslFI GGGAC 1 cut(s) 579
BslI CCNNNNNNNGG 8 cut(s) 242, 563, 575, 593, 608, 635, 671, 689
BsmFI GGGAC 1 cut(s) 579
BsnI GGCC 9 cut(s) 320, 545, 580, 586, 625, 634, 646, 676, 706
BsoBI CYCGRG 1 cut(s) 739
Bsp1286I GDGCHC 2 cut(s) 288, 389
Bsp143I GATC 3 cut(s) 297, 340, 755
Bsp19I CCATGG 2 cut(s) 591, 656
BspACI CCGC 6 cut(s) 116, 626, 665, 683, 707, 749
BspANI GGCC 9 cut(s) 320, 545, 580, 586, 625, 634, 646, 676, 706
BspLI GGNNCC 1 cut(s) 567
BspPI GGATC 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 461
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 9 cut(s) 575, 581, 591, 619, 629, 647, 656, 717, 740
BssMI GATC 3 cut(s) 297, 340, 755
BssT1I CCWWGG 4 cut(s) 591, 619, 629, 656
Bst2UI CCWGG 3 cut(s) 129, 576, 636
Bst4CI ACNGT 3 cut(s) 253, 259, 367
BstDEI CTNAG 2 cut(s) 279, 467
BstDSI CCRYGG 3 cut(s) 591, 656, 717
BstF5I GGATG 3 cut(s) 657, 666, 802
BstKTI GATC 3 cut(s) 300, 343, 758
BstMBI GATC 3 cut(s) 297, 340, 755
BstMCI CGRYCG 1 cut(s) 647
BstMWI GCNNNNNNNGC 5 cut(s) 631, 652, 661, 673, 682
BstNI CCWGG 3 cut(s) 129, 576, 636
BstSCI CCNGG 5 cut(s) 127, 562, 574, 634, 646
BstV1I GCAGC 1 cut(s) 40
BstV2I GAAGAC 1 cut(s) 497
BstXI CCANNNNNNTGG 1 cut(s) 787
BstZI CGGCCG 1 cut(s) 644
BsuRI GGCC 9 cut(s) 320, 545, 580, 586, 625, 634, 646, 676, 706
BtgI CCRYGG 3 cut(s) 591, 656, 717
BtsCI GGATG 3 cut(s) 657, 666, 802
BtsIMutI CAGTG 1 cut(s) 249
CaiI CAGNNNCTG 1 cut(s) 575
Cfr10I RCCGGY 1 cut(s) 461
Cfr13I GGNCC 5 cut(s) 131, 566, 578, 623, 674
CseI GACGC 2 cut(s) 33, 539
Csp6I GTAC 1 cut(s) 770
CviAII CATG 6 cut(s) 107, 289, 401, 547, 592, 657
CviQI GTAC 1 cut(s) 770
DdeI CTNAG 2 cut(s) 279, 467
DpnI GATC 3 cut(s) 299, 342, 757
DpnII GATC 3 cut(s) 297, 340, 755
DrdI GACNNNNNNGTC 1 cut(s) 129
DseDI GACNNNNNNGTC 1 cut(s) 129
EaeI YGGCCR 2 cut(s) 543, 644
EagI CGGCCG 1 cut(s) 644
EclXI CGGCCG 1 cut(s) 644
Eco130I CCWWGG 4 cut(s) 591, 619, 629, 656
Eco147I AGGCCT 1 cut(s) 634
Eco32I GATATC 1 cut(s) 731
Eco47I GGWCC 2 cut(s) 131, 566
Eco52I CGGCCG 1 cut(s) 644
Eco57I CTGAAG 2 cut(s) 206, 221
Eco88I CYCGRG 1 cut(s) 739
EcoO109I RGGNCCY 1 cut(s) 131
EcoRII CCWGG 3 cut(s) 127, 574, 634
EcoRV GATATC 1 cut(s) 731
EcoT14I CCWWGG 4 cut(s) 591, 619, 629, 656
ErhI CCWWGG 4 cut(s) 591, 619, 629, 656
FaeI CATG 6 cut(s) 110, 292, 404, 550, 595, 660
FaqI GGGAC 1 cut(s) 579
FatI CATG 6 cut(s) 106, 288, 400, 546, 591, 656
FauI CCCGC 1 cut(s) 123
FauNDI CATATG 1 cut(s) 688
Fnu4HI GCNGC 5 cut(s) 29, 626, 665, 707, 749
FokI GGATG 3 cut(s) 664, 673, 789
Fsp4HI GCNGC 5 cut(s) 29, 626, 665, 707, 749
FspBI CTAG 2 cut(s) 138, 811
GluI GCNGC 5 cut(s) 29, 626, 665, 707, 749
GsuI CTGGAG 1 cut(s) 111
HaeIII GGCC 9 cut(s) 320, 545, 580, 586, 625, 634, 646, 676, 706
HapII CCGG 3 cut(s) 462, 563, 647
HgaI GACGC 2 cut(s) 33, 539
Hin1II CATG 6 cut(s) 110, 292, 404, 550, 595, 660
HinfI GANTC 3 cut(s) 5, 275, 350
HpaII CCGG 3 cut(s) 462, 563, 647
HphI GGTGA 2 cut(s) 226, 307
Hpy166II GTNNAC 6 cut(s) 148, 226, 442, 473, 554, 772
Hpy188I TCNGA 5 cut(s) 302, 345, 358, 391, 603
Hpy188III TCNNGA 2 cut(s) 236, 739
Hpy8I GTNNAC 6 cut(s) 148, 226, 442, 473, 554, 772
HpyAV CCTTC 2 cut(s) 340, 346
HpyCH4III ACNGT 3 cut(s) 253, 259, 367
HpyCH4V TGCA 2 cut(s) 28, 110
HpyF10VI GCNNNNNNNGC 5 cut(s) 631, 652, 661, 673, 682
HpyF3I CTNAG 2 cut(s) 279, 467
Hsp92II CATG 6 cut(s) 110, 292, 404, 550, 595, 660
Kzo9I GATC 3 cut(s) 297, 340, 755
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 4 cut(s) 88, 448, 642, 651
MaeI CTAG 2 cut(s) 138, 811
MaeIII GTNAC 6 cut(s) 45, 253, 324, 449, 533, 711
MalI GATC 3 cut(s) 299, 342, 757
MboI GATC 3 cut(s) 297, 340, 755
MboII GAAGA 6 cut(s) 30, 199, 214, 499, 502, 508
MfeI CAATTG 1 cut(s) 669
MhlI GDGCHC 2 cut(s) 288, 389
MlsI TGGCCA 1 cut(s) 545
MluCI AATT 8 cut(s) 63, 160, 169, 307, 377, 430, 669, 793
MluNI TGGCCA 1 cut(s) 545
MlyI GAGTC 2 cut(s) 269, 359
MmeI TCCRAC 2 cut(s) 349, 626
Mox20I TGGCCA 1 cut(s) 545
MroXI GAANNNNTTC 1 cut(s) 63
MscI TGGCCA 1 cut(s) 545
MslI CAYNNNNRTG 2 cut(s) 105, 293
Msp20I TGGCCA 1 cut(s) 545
MspI CCGG 3 cut(s) 462, 563, 647
MspR9I CCNGG 5 cut(s) 129, 564, 576, 636, 648
MunI CAATTG 1 cut(s) 669
MvaI CCWGG 3 cut(s) 129, 576, 636
MwoI GCNNNNNNNGC 5 cut(s) 631, 652, 661, 673, 682
NciI CCSGG 2 cut(s) 564, 648
NcoI CCATGG 2 cut(s) 591, 656
NdeI CATATG 1 cut(s) 688
NdeII GATC 3 cut(s) 297, 340, 755
NlaIII CATG 6 cut(s) 110, 292, 404, 550, 595, 660
NlaIV GGNNCC 1 cut(s) 567
NmeAIII GCCGAG 1 cut(s) 562
NmuCI GTSAC 1 cut(s) 45
PceI AGGCCT 1 cut(s) 634
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 1 cut(s) 5
PflMI CCANNNNNTGG 2 cut(s) 575, 635
PfoI TCCNGGA 1 cut(s) 562
PkrI GCNGC 5 cut(s) 30, 627, 666, 708, 750
PleI GAGTC 2 cut(s) 269, 358
PpsI GAGTC 2 cut(s) 269, 358
PpuMI RGGWCCY 1 cut(s) 131
Psp5II RGGWCCY 1 cut(s) 131
Psp6I CCWGG 3 cut(s) 127, 574, 634
PspGI CCWGG 3 cut(s) 127, 574, 634
PspN4I GGNNCC 1 cut(s) 567
PspPI GGNCC 5 cut(s) 131, 566, 578, 623, 674
PspPPI RGGWCCY 1 cut(s) 131
PstNI CAGNNNCTG 1 cut(s) 575
RsaI GTAC 1 cut(s) 771
RsaNI GTAC 1 cut(s) 770
RseI CAYNNNNRTG 2 cut(s) 105, 293
SatI GCNGC 5 cut(s) 29, 626, 665, 707, 749
Sau3AI GATC 3 cut(s) 297, 340, 755
Sau96I GGNCC 5 cut(s) 131, 566, 578, 623, 674
SchI GAGTC 2 cut(s) 269, 359
ScrFI CCNGG 5 cut(s) 129, 564, 576, 636, 648
SduI GDGCHC 2 cut(s) 288, 389
SfaNI GCATC 4 cut(s) 88, 448, 642, 651
SfiI GGCCNNNNNGGCC 1 cut(s) 631
SinI GGWCC 2 cut(s) 131, 566
SmiMI CAYNNNNRTG 2 cut(s) 105, 293
Sse9I AATT 8 cut(s) 63, 160, 169, 307, 377, 430, 669, 793
SseBI AGGCCT 1 cut(s) 634
SsiI CCGC 6 cut(s) 116, 626, 665, 683, 707, 749
SspMI CTAG 2 cut(s) 138, 811
StuI AGGCCT 1 cut(s) 634
StyD4I CCNGG 5 cut(s) 127, 562, 574, 634, 646
StyI CCWWGG 4 cut(s) 591, 619, 629, 656
TaaI ACNGT 3 cut(s) 253, 259, 367
TaqI TCGA 3 cut(s) 121, 339, 758
TasI AATT 8 cut(s) 63, 160, 169, 307, 377, 430, 669, 793
TauI GCSGC 4 cut(s) 628, 667, 709, 751
TfiI GAWTC 1 cut(s) 5
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 1 cut(s) 45
TseI GCWGC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 45
TspDTI ATGAA 1 cut(s) 411
TspGWI ACGGA 2 cut(s) 734, 741
TspRI CASTG 1 cut(s) 256
Van91I CCANNNNNTGG 2 cut(s) 575, 635
VpaK11BI GGWCC 2 cut(s) 131, 566
XapI RAATTY 1 cut(s) 377
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 2 cut(s) 138, 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.