FvH4_2g24230

Endonuclease/Exonuclease/phosphatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010396
Physical Location & Seq
Forward (+)
19779314 .. 19783124
3811 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24230.t1

Sequence Viewer

Length: 1488 bp
ATGCTCAAAAACCTCCTCCGCCTCTGCTCCCGTCTCCGGTGGCCGGCCCGCCGCCGCTCCCCGACCAAAGTGGTCATCAAACGCTTCTCCAAAACCAAAACCAACTCCAACTCCCACCTCCACCACGAACCCACTTCCAACGGCCCCTCGTCGGCGACGGTCCACCCCAACGGCCAGCCGGAAACAGAGAATCCCCCGCCGCCGCGGCCCATTCGGGTCGCCACTTTCAACGCAGCACTTTTCTCAATGGCGCCGGCGATTCCGACGCCGGAGAAACCGGAGCGAGACGAAAACGGCCTCGTTAAGGATATAAAGAACGCTACTTGCAGACCCAAATCGGCGATGGACCGACCCAAGAGCATCTTAAAGCAGTCGCCTTTACACCCAAACGCGATGAAGAGCTCAGAGAATCAGAAAACGCAACAGAAGTTCGTGAAATCCAAGATGAGGGTCTCGATCAATTTGCCGGATAATGAAATCTCATTGCTCAAAAGCAGACAGCTGAGCTTTGCTGAAGGCAGAGAGCTTGATACTAGTAATTCTTTATCTTCTTTCTCCCCAAGTCGAGTTTCAAAAGGAAAAGGTCCGTTGAAGCCGAGTGTGAGCTTTCCTAATAATATTGGGAACTGTGAAAATGGAGAGTACTATACAAGTCATAGGACTGTTCTTGAGGTTTTGAGGGAATTGGATGCTGACATTTTGGCTCTGCAAGATGTGAAAGCAGAGGAGGAGAAGGAAATGAAGCCTCTTTCGGATTTGGCTGCTGCTTTGGGGATGAACTATGTGTTTGCTGAGAGCTGGGCGCCGGAGTACGGCAATGCCATCTTGTCGAAATGGCCGATCAAGCGGTCTAAGGTTCAGAAAATCTTTGATGACTCTGATTTCAGGAACGTTCTGAAAGCCACCATTGATGTACCCCAAGCCGGAGAAGTCAACTTCCAGTGCACCCACCTTGATCATCTTGATGAGAACTGGAGGATGAGGCAGGTTAACGCTATAATCCAATCGAGCAATGAGCCTCACATCTTGGCCGGAGGTCTCAATTCACTAGAGGAATCAGATTATTCTCAAGAAAGATGGACTGACATAGTAAAGTATTACGAGGAGATGGGAAAGCCAACACCCAAGGTCGAGGTAATGAGGTACTTGAAGAGCAAGCAGTACACAGATGCCAAGGACTTTGAAGGAGAATATGAGTCGGTAGTCATGATCGCCAAAGGCCAAAGTGTGCAGGGGACATGTAAGTATGGAACGAGGGTAGATTACATATTAGCATCCTCAAATTCGCCATACAAGTTTGTTCCCGGATCATACTCTGTCTTCTCATCCAAAGGGACATCCGATCATCATATAGTAAGAGTTGATGTGCTCAAATCGGTAAGTTGTTGTGATCCAGGAAACGTCTCCACACGCAGGCAAAGAAAACAGAAAGTCATAAAGATAACGGAGACTACTCCATCAAAAGGCATATGGAAAACACAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

496

Amino Acids

55.7

Weight (kDa)

9.37

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 215 - 449 6.2e-12 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 976
AccB1I GGYRCC 2 cut(s) 250, 802
AccBSI CCGCTC 1 cut(s) 57
AccII CGCG 2 cut(s) 205, 392
AciI CCGC 9 cut(s) 19, 49, 52, 55, 197, 200, 203, 205, 847
AclI AACGTT 1 cut(s) 891
AclWI GGATC 2 cut(s) 1315, 1385
AcoI YGGCCR 4 cut(s) 41, 172, 836, 1029
AcsI RAATTY 1 cut(s) 1282
AcuI CTGAAG 1 cut(s) 534
AcyI GRCGYC 3 cut(s) 251, 266, 803
AfaI GTAC 5 cut(s) 644, 812, 915, 1145, 1163
AfiI CCNNNNNNNGG 9 cut(s) 36, 43, 151, 304, 447, 812, 923, 1413, 1463
AflIII ACRYGT 1 cut(s) 1238
AgsI TTSAA 5 cut(s) 229, 573, 592, 1150, 1184
AhlI ACTAGT 1 cut(s) 533
AjnI CCWGG 1 cut(s) 1393
AjuI GAANNNNNNNTTGG 2 cut(s) 553, 585
AluBI AGCT 6 cut(s) 402, 502, 507, 526, 606, 798
AluI AGCT 6 cut(s) 402, 502, 507, 526, 606, 798
Alw21I GWGCWC 3 cut(s) 404, 947, 1371
Alw26I GTCTC 6 cut(s) 38, 279, 457, 1043, 1408, 1442
Alw44I GTGCAC 1 cut(s) 943
AlwI GGATC 2 cut(s) 1315, 1385
AoxI GGCC 9 cut(s) 41, 45, 142, 172, 206, 295, 836, 1029, 1219
ApaLI GTGCAC 1 cut(s) 943
ApeKI GCWGC 3 cut(s) 233, 761, 764
ApoI RAATTY 1 cut(s) 1282
AspLEI GCGC 2 cut(s) 253, 805
AspS9I GGNCC 6 cut(s) 46, 143, 160, 207, 346, 584
AsuC2I CCSGG 1 cut(s) 1305
AvaII GGWCC 3 cut(s) 160, 346, 584
BaeGI GKGCMC 1 cut(s) 947
BanI GGYRCC 2 cut(s) 250, 802
BanII GRGCYC 1 cut(s) 404
BbsI GAAGAC 1 cut(s) 1312
Bbv12I GWGCWC 3 cut(s) 404, 947, 1371
BbvI GCAGC 3 cut(s) 245, 748, 751
BccI CCATC 5 cut(s) 337, 830, 1071, 1102, 1465
BceAI ACGGC 4 cut(s) 157, 187, 310, 829
BcgI CGANNNNNNTGC 2 cut(s) 445, 479
BciT130I CCWGG 1 cut(s) 1395
BclI TGATCA 1 cut(s) 955
BcnI CCSGG 1 cut(s) 1305
BcoDI GTCTC 6 cut(s) 38, 279, 457, 1043, 1408, 1442
BcuI ACTAGT 1 cut(s) 533
BfaI CTAG 2 cut(s) 534, 1049
BfoI RGCGCY 2 cut(s) 254, 806
BfuAI ACCTGC 1 cut(s) 976
BglI GCCNNNNNGGC 1 cut(s) 205
BisI GCNGC 8 cut(s) 52, 55, 200, 203, 206, 234, 762, 765
BlpI GCTNAGC 1 cut(s) 503
BlsI GCNGC 8 cut(s) 53, 56, 201, 204, 207, 235, 763, 766
BmcAI AGTACT 1 cut(s) 644
Bme1390I CCNGG 2 cut(s) 1305, 1395
Bme18I GGWCC 3 cut(s) 160, 346, 584
BmgT120I GGNCC 6 cut(s) 46, 143, 160, 207, 346, 584
BmiI GGNNCC 3 cut(s) 145, 252, 804
BmrFI CCNGG 2 cut(s) 1305, 1395
BmsI GCATC 4 cut(s) 369, 679, 1159, 1283
BpiI GAAGAC 1 cut(s) 1312
BpmI CTGGAG 1 cut(s) 994
Bpu1102I GCTNAGC 1 cut(s) 503
BpuEI CTTGAG 2 cut(s) 689, 1053
BpuMI CCSGG 1 cut(s) 1305
BsaHI GRCGYC 3 cut(s) 251, 266, 803
BsaI GGTCTC 2 cut(s) 457, 1043
BsaJI CCNNGG 3 cut(s) 203, 1125, 1173
BsaWI WCCGGW 2 cut(s) 36, 277
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 9 cut(s) 36, 43, 151, 304, 447, 812, 923, 1413, 1463
Bse118I RCCGGY 2 cut(s) 43, 253
Bse1I ACTGG 2 cut(s) 940, 977
Bse3DI GCAATG 3 cut(s) 482, 823, 1018
BseBI CCWGG 1 cut(s) 1395
BseDI CCNNGG 3 cut(s) 203, 1125, 1173
BseGI GGATG 6 cut(s) 694, 780, 984, 1274, 1325, 1337
BseLI CCNNNNNNNGG 9 cut(s) 36, 43, 151, 304, 447, 812, 923, 1413, 1463
BseMI GCAATG 3 cut(s) 482, 823, 1018
BseMII CTCAG 3 cut(s) 417, 494, 783
BseNI ACTGG 2 cut(s) 940, 977
BseRI GAGGAG 4 cut(s) 5, 740, 743, 1118
BseSI GKGCMC 1 cut(s) 947
BseXI GCAGC 3 cut(s) 245, 748, 751
BseYI CCCAGC 1 cut(s) 798
BsgI GTGCAG 1 cut(s) 1250
Bsh1236I CGCG 2 cut(s) 205, 392
BshFI GGCC 9 cut(s) 43, 47, 144, 174, 208, 297, 838, 1031, 1221
BshNI GGYRCC 2 cut(s) 250, 802
BsiHKAI GWGCWC 3 cut(s) 404, 947, 1371
BslFI GGGAC 2 cut(s) 1249, 1348
BslI CCNNNNNNNGG 9 cut(s) 36, 43, 151, 304, 447, 812, 923, 1413, 1463
BsmAI GTCTC 6 cut(s) 38, 279, 457, 1043, 1408, 1442
BsmBI CGTCTC 3 cut(s) 38, 279, 1408
BsmFI GGGAC 2 cut(s) 1249, 1348
BsnI GGCC 9 cut(s) 43, 47, 144, 174, 208, 297, 838, 1031, 1221
Bso31I GGTCTC 2 cut(s) 457, 1043
Bsp1286I GDGCHC 3 cut(s) 404, 947, 1371
Bsp143I GATC 7 cut(s) 456, 840, 955, 1209, 1307, 1342, 1390
Bsp1720I GCTNAGC 1 cut(s) 503
BspACI CCGC 9 cut(s) 19, 49, 52, 55, 197, 200, 203, 205, 847
BspANI GGCC 9 cut(s) 43, 47, 144, 174, 208, 297, 838, 1031, 1221
BspCNI CTCAG 3 cut(s) 416, 495, 784
BspFNI CGCG 2 cut(s) 205, 392
BspHI TCATGA 1 cut(s) 1206
BspLI GGNNCC 3 cut(s) 145, 252, 804
BspMI ACCTGC 1 cut(s) 976
BspPI GGATC 2 cut(s) 1315, 1385
BspQI GCTCTTC 2 cut(s) 392, 1145
BspT107I GGYRCC 2 cut(s) 250, 802
BspTNI GGTCTC 2 cut(s) 457, 1043
BsrBI CCGCTC 1 cut(s) 57
BsrDI GCAATG 3 cut(s) 482, 823, 1018
BsrFI RCCGGY 2 cut(s) 43, 253
BsrI ACTGG 2 cut(s) 940, 977
BssAI RCCGGY 2 cut(s) 43, 253
BssECI CCNNGG 3 cut(s) 203, 1125, 1173
BssMI GATC 7 cut(s) 456, 840, 955, 1209, 1307, 1342, 1390
BssNI GRCGYC 3 cut(s) 251, 266, 803
BssT1I CCWWGG 2 cut(s) 1125, 1173
Bst2UI CCWGG 1 cut(s) 1395
Bst4CI ACNGT 3 cut(s) 160, 629, 664
Bst6I CTCTTC 2 cut(s) 392, 1145
BstACI GRCGYC 3 cut(s) 251, 266, 803
BstC8I GCNNGC 6 cut(s) 45, 49, 176, 255, 1157, 1415
BstDEI CTNAG 4 cut(s) 403, 503, 792, 852
BstDSI CCRYGG 1 cut(s) 203
BstENI CCTNNNNNAGG 1 cut(s) 302
BstF5I GGATG 6 cut(s) 694, 780, 984, 1274, 1325, 1337
BstFNI CGCG 2 cut(s) 205, 392
BstH2I RGCGCY 2 cut(s) 254, 806
BstHHI GCGC 2 cut(s) 253, 805
BstKTI GATC 7 cut(s) 459, 843, 958, 1212, 1310, 1345, 1393
BstMAI GTCTC 6 cut(s) 38, 279, 457, 1043, 1408, 1442
BstMBI GATC 7 cut(s) 456, 840, 955, 1209, 1307, 1342, 1390
BstMWI GCNNNNNNNGC 2 cut(s) 205, 844
BstNI CCWGG 1 cut(s) 1395
BstNSI RCATGY 1 cut(s) 1242
BstSCI CCNGG 2 cut(s) 1303, 1393
BstSLI GKGCMC 1 cut(s) 947
BstUI CGCG 2 cut(s) 205, 392
BstV1I GCAGC 3 cut(s) 245, 748, 751
BstV2I GAAGAC 1 cut(s) 1312
BsuRI GGCC 9 cut(s) 43, 47, 144, 174, 208, 297, 838, 1031, 1221
BtgI CCRYGG 1 cut(s) 203
BtgZI GCGATG 2 cut(s) 356, 407
BtsCI GGATG 6 cut(s) 694, 780, 984, 1274, 1325, 1337
BtsIMutI CAGTG 1 cut(s) 947
BveI ACCTGC 1 cut(s) 976
Cac8I GCNNGC 6 cut(s) 45, 49, 176, 255, 1157, 1415
CciI TCATGA 1 cut(s) 1206
CfoI GCGC 2 cut(s) 253, 805
Cfr10I RCCGGY 2 cut(s) 43, 253
Cfr13I GGNCC 6 cut(s) 46, 143, 160, 207, 346, 584
Cfr42I CCGCGG 1 cut(s) 206
CseI GACGC 1 cut(s) 274
Csp6I GTAC 5 cut(s) 643, 811, 914, 1144, 1162
CviAII CATG 3 cut(s) 1207, 1239, 1485
CviQI GTAC 5 cut(s) 643, 811, 914, 1144, 1162
DdeI CTNAG 4 cut(s) 403, 503, 792, 852
DinI GGCGCC 2 cut(s) 252, 804
DpnI GATC 7 cut(s) 458, 842, 957, 1211, 1309, 1344, 1392
DpnII GATC 7 cut(s) 456, 840, 955, 1209, 1307, 1342, 1390
EaeI YGGCCR 4 cut(s) 41, 172, 836, 1029
Eam1104I CTCTTC 2 cut(s) 392, 1145
EarI CTCTTC 2 cut(s) 392, 1145
EciI GGCGGA 1 cut(s) 8
Ecl136II GAGCTC 1 cut(s) 402
Eco130I CCWWGG 2 cut(s) 1125, 1173
Eco24I GRGCYC 1 cut(s) 404
Eco31I GGTCTC 2 cut(s) 457, 1043
Eco47I GGWCC 3 cut(s) 160, 346, 584
Eco53kI GAGCTC 1 cut(s) 402
Eco57I CTGAAG 1 cut(s) 534
EcoICRI GAGCTC 1 cut(s) 402
EcoNI CCTNNNNNAGG 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 1393
EcoT14I CCWWGG 2 cut(s) 1125, 1173
EcoT38I GRGCYC 1 cut(s) 404
EgeI GGCGCC 2 cut(s) 252, 804
EheI GGCGCC 2 cut(s) 252, 804
ErhI CCWWGG 2 cut(s) 1125, 1173
Esp3I CGTCTC 3 cut(s) 38, 279, 1408
FaeI CATG 3 cut(s) 1210, 1242, 1488
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 2 cut(s) 1249, 1348
FatI CATG 3 cut(s) 1206, 1238, 1484
FauI CCCGC 2 cut(s) 56, 204
FauNDI CATATG 1 cut(s) 1469
FbaI TGATCA 1 cut(s) 955
Fnu4HI GCNGC 8 cut(s) 52, 55, 200, 203, 206, 234, 762, 765
FokI GGATG 6 cut(s) 701, 787, 991, 1261, 1312, 1324
FriOI GRGCYC 1 cut(s) 404
FseI GGCCGGCC 1 cut(s) 47
Fsp4HI GCNGC 8 cut(s) 52, 55, 200, 203, 206, 234, 762, 765
FspBI CTAG 2 cut(s) 534, 1049
GlaI GCGC 2 cut(s) 252, 804
GluI GCNGC 8 cut(s) 52, 55, 200, 203, 206, 234, 762, 765
GsaI CCCAGC 1 cut(s) 802
GsuI CTGGAG 1 cut(s) 994
HaeII RGCGCY 2 cut(s) 254, 806
HaeIII GGCC 9 cut(s) 43, 47, 144, 174, 208, 297, 838, 1031, 1221
HgaI GACGC 1 cut(s) 274
HhaI GCGC 2 cut(s) 253, 805
Hin1I GRCGYC 3 cut(s) 251, 266, 803
Hin1II CATG 3 cut(s) 1210, 1242, 1488
Hin6I GCGC 2 cut(s) 251, 803
HinP1I GCGC 2 cut(s) 251, 803
HincII GTYRAC 2 cut(s) 934, 991
HindII GTYRAC 2 cut(s) 934, 991
HinfI GANTC 6 cut(s) 190, 259, 409, 875, 1055, 1196
HpaI GTTAAC 1 cut(s) 991
Hpy166II GTNNAC 5 cut(s) 163, 934, 945, 991, 1164
Hpy188I TCNGA 9 cut(s) 264, 406, 414, 754, 861, 880, 897, 1060, 1342
Hpy188III TCNNGA 7 cut(s) 433, 454, 668, 886, 962, 1070, 1207
Hpy8I GTNNAC 5 cut(s) 163, 934, 945, 991, 1164
Hpy99I CGWCG 3 cut(s) 154, 160, 268
HpyAV CCTTC 3 cut(s) 509, 727, 1178
HpyCH4III ACNGT 3 cut(s) 160, 629, 664
HpyCH4IV ACGT 2 cut(s) 891, 1401
HpyCH4V TGCA 4 cut(s) 327, 709, 945, 1231
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 844
HpyF3I CTNAG 4 cut(s) 403, 503, 792, 852
HpySE526I ACGT 2 cut(s) 891, 1401
Hsp92I GRCGYC 3 cut(s) 251, 266, 803
Hsp92II CATG 3 cut(s) 1210, 1242, 1488
HspAI GCGC 2 cut(s) 251, 803
KasI GGCGCC 2 cut(s) 250, 802
KroI GCCGGC 2 cut(s) 43, 253
KroNI GCCGGC 2 cut(s) 45, 255
Ksp22I TGATCA 1 cut(s) 955
KspAI GTTAAC 1 cut(s) 991
KspI CCGCGG 1 cut(s) 206
Kzo9I GATC 7 cut(s) 456, 840, 955, 1209, 1307, 1342, 1390
LguI GCTCTTC 2 cut(s) 392, 1145
LmnI GCTCC 3 cut(s) 32, 62, 280
Lsp1109I GCAGC 3 cut(s) 245, 748, 751
LweI GCATC 4 cut(s) 369, 679, 1159, 1283
MaeI CTAG 2 cut(s) 534, 1049
MaeII ACGT 2 cut(s) 891, 1401
MalI GATC 7 cut(s) 458, 842, 957, 1211, 1309, 1344, 1392
MbiI CCGCTC 1 cut(s) 57
MboI GATC 7 cut(s) 456, 840, 955, 1209, 1307, 1342, 1390
MboII GAAGA 4 cut(s) 409, 540, 1162, 1312
MhlI GDGCHC 3 cut(s) 404, 947, 1371
MluCI AATT 5 cut(s) 460, 538, 683, 1042, 1282
Mly113I GGCGCC 2 cut(s) 251, 803
MlyI GAGTC 2 cut(s) 869, 1205
MmeI TCCRAC 3 cut(s) 132, 162, 287
MreI CGCCGGCG 1 cut(s) 253
MroNI GCCGGC 2 cut(s) 43, 253
MseI TTAA 3 cut(s) 303, 365, 990
MslI CAYNNNNRTG 2 cut(s) 963, 1483
MspA1I CMGCKG 2 cut(s) 205, 502
MspR9I CCNGG 2 cut(s) 1305, 1395
MvaI CCWGG 1 cut(s) 1395
MvnI CGCG 2 cut(s) 205, 392
MwoI GCNNNNNNNGC 2 cut(s) 205, 844
NaeI GCCGGC 2 cut(s) 45, 255
NarI GGCGCC 2 cut(s) 251, 803
NciI CCSGG 1 cut(s) 1305
NdeI CATATG 1 cut(s) 1469
NdeII GATC 7 cut(s) 456, 840, 955, 1209, 1307, 1342, 1390
NgoMIV GCCGGC 2 cut(s) 43, 253
NlaIII CATG 3 cut(s) 1210, 1242, 1488
NlaIV GGNNCC 3 cut(s) 145, 252, 804
NmeAIII GCCGAG 1 cut(s) 621
NspI RCATGY 1 cut(s) 1242
PagI TCATGA 1 cut(s) 1206
PciI ACATGT 1 cut(s) 1238
PciSI GCTCTTC 2 cut(s) 392, 1145
PcsI WCGNNNNNNNCGW 1 cut(s) 836
PdiI GCCGGC 2 cut(s) 45, 255
PfeI GAWTC 4 cut(s) 190, 259, 409, 1055
PfoI TCCNGGA 2 cut(s) 1303, 1393
PkrI GCNGC 8 cut(s) 53, 56, 201, 204, 207, 235, 763, 766
PleI GAGTC 2 cut(s) 869, 1204
PluTI GGCGCC 2 cut(s) 254, 806
PpsI GAGTC 2 cut(s) 869, 1204
PscI ACATGT 1 cut(s) 1238
Psp124BI GAGCTC 1 cut(s) 404
Psp1406I AACGTT 1 cut(s) 891
Psp6I CCWGG 1 cut(s) 1393
PspFI CCCAGC 1 cut(s) 798
PspGI CCWGG 1 cut(s) 1393
PspN4I GGNNCC 3 cut(s) 145, 252, 804
PspPI GGNCC 6 cut(s) 46, 143, 160, 207, 346, 584
PvuII CAGCTG 1 cut(s) 502
RigI GGCCGGCC 1 cut(s) 47
RsaI GTAC 5 cut(s) 644, 812, 915, 1145, 1163
RsaNI GTAC 5 cut(s) 643, 811, 914, 1144, 1162
RseI CAYNNNNRTG 2 cut(s) 963, 1483
SacI GAGCTC 1 cut(s) 404
SacII CCGCGG 1 cut(s) 206
SapI GCTCTTC 2 cut(s) 392, 1145
SaqAI TTAA 3 cut(s) 303, 365, 990
SatI GCNGC 8 cut(s) 52, 55, 200, 203, 206, 234, 762, 765
Sau3AI GATC 7 cut(s) 456, 840, 955, 1209, 1307, 1342, 1390
Sau96I GGNCC 6 cut(s) 46, 143, 160, 207, 346, 584
ScaI AGTACT 1 cut(s) 644
SchI GAGTC 2 cut(s) 869, 1205
ScrFI CCNGG 2 cut(s) 1305, 1395
SduI GDGCHC 3 cut(s) 404, 947, 1371
SfaNI GCATC 4 cut(s) 369, 679, 1159, 1283
SfoI GGCGCC 2 cut(s) 252, 804
Sfr303I CCGCGG 1 cut(s) 206
SgrAI CRCCGGYG 1 cut(s) 253
SgrBI CCGCGG 1 cut(s) 206
SinI GGWCC 3 cut(s) 160, 346, 584
SmiMI CAYNNNNRTG 2 cut(s) 963, 1483
SmlI CTYRAG 2 cut(s) 668, 1068
SmoI CTYRAG 2 cut(s) 668, 1068
SpeI ACTAGT 1 cut(s) 533
Sse9I AATT 5 cut(s) 460, 538, 683, 1042, 1282
SsiI CCGC 9 cut(s) 19, 49, 52, 55, 197, 200, 203, 205, 847
SspDI GGCGCC 2 cut(s) 250, 802
SspI AATATT 1 cut(s) 619
SspMI CTAG 2 cut(s) 534, 1049
SstI GAGCTC 1 cut(s) 404
StyD4I CCNGG 2 cut(s) 1303, 1393
StyI CCWWGG 2 cut(s) 1125, 1173
TaaI ACNGT 3 cut(s) 160, 629, 664
TaiI ACGT 2 cut(s) 894, 1404
TaqI TCGA 5 cut(s) 455, 565, 830, 1007, 1131
TaqII GACCGA 1 cut(s) 363
TasI AATT 5 cut(s) 460, 538, 683, 1042, 1282
TatI WGTACW 2 cut(s) 642, 1161
TauI GCSGC 5 cut(s) 54, 57, 202, 205, 208
TfiI GAWTC 4 cut(s) 190, 259, 409, 1055
Tru1I TTAA 3 cut(s) 303, 365, 990
Tru9I TTAA 3 cut(s) 303, 365, 990
TscAI CASTG 1 cut(s) 947
TseI GCWGC 3 cut(s) 233, 761, 764
TspDTI ATGAA 4 cut(s) 410, 489, 755, 791
TspGWI ACGGA 2 cut(s) 576, 1460
TspRI CASTG 1 cut(s) 947
VneI GTGCAC 1 cut(s) 943
VpaK11BI GGWCC 3 cut(s) 160, 346, 584
XagI CCTNNNNNAGG 1 cut(s) 302
XapI RAATTY 1 cut(s) 1282
XceI RCATGY 1 cut(s) 1242
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XspI CTAG 2 cut(s) 534, 1049
ZrmI AGTACT 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.