pycom03g21670

Endonuclease/Exonuclease/phosphatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010396
Physical Location & Seq
Forward (+)
21928952 .. 21932704
3753 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g21670.1

Sequence Viewer

Length: 1518 bp
ATGCTCTCTCTAACTCCCACCAACAAAAACACCACCACAATGCTCAAACTCCTCAGCAGGAAGCTCCTCCGCCTCTGCTCCCACCTCCGCTGGCGGGTCTGCCGCCGCTCCCGCCCCAAAGTCGTCATAAAAGTTTTCGGGAAAACAAGCTCCAGAAAATCCCAACCCGAAAATGAAGCCAGTGGCACTCATGCGCCGGCGGTAGTACACCCCAATACCGACTTAAAAAAAGAGCGGCCGATTCGGGTGGCGACTTTCAACGCCGCCCTCTTCTCAATGGCGCCGGCGGTTCCCAAAGCCGAGAAATTCGGGAACTTGGACGGCGAAAATGGAGGTGGTAGCTTGAAGATTCATAAACACGTCAGTCGAACAAAGTCTTCGGGAAGCGAACGTCCGAAGAGTATTCTGAAACAGAGTCCTCTGCATCCGAATTCGATGAACAACTCGGAGAATCTCACGAAGCAGCAGAAGTTTTCGAAATCCAAGTTAAGGGTTTCGATCAATCTTCCGGATAACGAGATTTCCCTGCTGAAAAACCGGCAATTGAGCTTTTCGGAAGGCAGAGCAAAGGAAGATGCTTTCTCTCCGGTAACTGGAAAATCTCCTCTGAGATCGTCGAGTGTGAGGTTTTCGGCAGTTTTGAGAAGTGGGGCGACGGCGGATGGAGATTTGTACAGAAGTCATCGGACGGTTCTTGAAGTTTTGAGAGAGCTGGATGCTGATGTTCTGGCGCTGCAGGATGTGAAGGCGGAGGAGGAGAAGGATATGAAGCCGCTGTCAGACTTGGCGGCGGCGTTGGGGATGAACTATGTTTTTGCCGAGAGCTGGGCGCCGGAGTACGGCAACGCCATCTTGTCGAAATGGCCGATTAAGCGGTCCAAGGTCGAGAAAATCTTCGATGACACCGATTTCAGGAATGTCCTGAAGGCCACTATTGATGTTCCGGAAGCAGGAGAAGTCAACTTCCATTGCACCCACCTCGATCATCTTGATGAGAGCTGGAGGATGAGGCAGATTAATGCTATAATCCAATCAAGCAATGAGCCCCACATCTTGGCTGGAGGCCTCAATTCTTTGGATGAGTCAGACTACTCTCAAGAAAGATGGACTGATATTGTAAAGTATTATGAGGATATGGGTAAACCAAGGCCTAAGGTTGAAGTAATGAGATACTTGAAGAGCAAGCACTACACAGATGCAAAGGACTTTGAAGGAGAATATGAGTCAGTAGTTATGATTGCCAAAGGCCAAAGTGTGCAGGGAACATGCAAGTACGGAACGAGAGTAGATTATATATTAGCATCTGCAAACTCACCTTACAAATTTGTTCCTGGATCGATCTCAGTGTTTTCTTCCAAAGGGACATCAGATCATCATATAGTAAGAGTTGATGTTGTAAAAGTGGATAATTGTGGTGAAGAAAATAACAGCAGAAGAAGGCAGCTGAAGCAAAAAGTTACAAAGATAACAAACCCTACTTCCCAGTCAAAAGGAATATGGAAAATCCCACATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

506

Amino Acids

56.69

Weight (kDa)

9.6

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos_PGAP2IP PF23226 220 - 357 5.3e-06 PGAP2IP, C-terminal nuclease-like domain
Exo_endo_phos PF03372 224 - 458 2.4e-12 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 280, 829
AccB7I CCANNNNNTGG 1 cut(s) 1054
AccBSI CCGCTC 2 cut(s) 108, 235
AccIII TCCGGA 2 cut(s) 508, 943
AclWI GGATC 1 cut(s) 1342
AcoI YGGCCR 2 cut(s) 236, 863
AcsI RAATTY 3 cut(s) 305, 430, 1322
AcuI CTGAAG 2 cut(s) 944, 1466
AcyI GRCGYC 2 cut(s) 281, 830
AfaI GTAC 4 cut(s) 207, 674, 839, 1274
AfiI CCNNNNNNNGG 8 cut(s) 94, 489, 593, 825, 839, 912, 950, 1054
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 6 cut(s) 259, 346, 698, 1160, 1177, 1211
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 1 cut(s) 1330
AluBI AGCT 8 cut(s) 64, 150, 342, 549, 712, 825, 999, 1444
AluI AGCT 8 cut(s) 64, 150, 342, 549, 712, 825, 999, 1444
AlwI GGATC 1 cut(s) 1342
Aor13HI TCCGGA 2 cut(s) 508, 943
AoxI GGCC 6 cut(s) 236, 863, 927, 1063, 1148, 1246
ApeKI GCWGC 3 cut(s) 463, 733, 1441
ApoI RAATTY 3 cut(s) 305, 430, 1322
AseI ATTAAT 1 cut(s) 1017
Asp700I GAANNNNTTC 1 cut(s) 893
AspLEI GCGC 4 cut(s) 196, 283, 733, 832
AspS9I GGNCC 1 cut(s) 876
AsuHPI GGTGA 2 cut(s) 1305, 1427
AsuII TTCGAA 1 cut(s) 476
AvaII GGWCC 1 cut(s) 876
AxyI CCTNAGG 1 cut(s) 1152
BanI GGYRCC 2 cut(s) 280, 829
BanII GRGCYC 1 cut(s) 1047
BbsI GAAGAC 1 cut(s) 369
BbvCI CCTCAGC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 475, 720, 1453
BccI CCATC 3 cut(s) 656, 857, 1098
BceAI ACGGC 3 cut(s) 337, 672, 856
BcgI CGANNNNNNTGC 2 cut(s) 961, 995
BciT130I CCWGG 1 cut(s) 1332
BfmI CTRYAG 1 cut(s) 734
BfoI RGCGCY 3 cut(s) 284, 734, 833
Bme1390I CCNGG 1 cut(s) 1332
Bme18I GGWCC 1 cut(s) 876
BmgBI CACGTC 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 876
BmiI GGNNCC 3 cut(s) 282, 291, 831
BmrFI CCNGG 1 cut(s) 1332
BmrI ACTGGG 1 cut(s) 1477
BmsI GCATC 5 cut(s) 433, 565, 706, 1186, 1310
BmuI ACTGGG 1 cut(s) 1477
BpiI GAAGAC 1 cut(s) 369
BpmI CTGGAG 3 cut(s) 136, 1021, 1080
Bpu10I CCTNAGC 1 cut(s) 53
Bpu14I TTCGAA 1 cut(s) 476
BpuEI CTTGAG 1 cut(s) 1080
Bsa29I ATCGAT 1 cut(s) 1337
BsaHI GRCGYC 2 cut(s) 281, 830
BsaJI CCNNGG 2 cut(s) 879, 1145
BsaWI WCCGGW 3 cut(s) 508, 586, 943
Bsc4I CCNNNNNNNGG 8 cut(s) 94, 489, 593, 825, 839, 912, 950, 1054
Bse118I RCCGGY 3 cut(s) 196, 283, 537
Bse1I ACTGG 3 cut(s) 180, 598, 1483
Bse21I CCTNAGG 1 cut(s) 1152
Bse3DI GCAATG 2 cut(s) 967, 1045
BseAI TCCGGA 2 cut(s) 508, 943
BseBI CCWGG 1 cut(s) 1332
BseCI ATCGAT 1 cut(s) 1337
BseDI CCNNGG 2 cut(s) 879, 1145
BseGI GGATG 7 cut(s) 424, 667, 721, 745, 807, 1011, 1084
BseLI CCNNNNNNNGG 8 cut(s) 94, 489, 593, 825, 839, 912, 950, 1054
BseMI GCAATG 2 cut(s) 967, 1045
BseMII CTCAG 3 cut(s) 67, 599, 1356
BseNI ACTGG 3 cut(s) 180, 598, 1483
BseRI GAGGAG 5 cut(s) 41, 56, 594, 767, 770
BseX3I CGGCCG 1 cut(s) 236
BseXI GCAGC 3 cut(s) 475, 720, 1453
BseYI CCCAGC 1 cut(s) 825
BsgI GTGCAG 1 cut(s) 1277
Bsh1285I CGRYCG 1 cut(s) 239
BshFI GGCC 6 cut(s) 238, 865, 929, 1065, 1150, 1248
BshNI GGYRCC 2 cut(s) 280, 829
BshVI ATCGAT 1 cut(s) 1337
BsiEI CGRYCG 1 cut(s) 239
BsiSI CCGG 7 cut(s) 197, 284, 509, 538, 587, 833, 944
BslFI GGGAC 1 cut(s) 1375
BslI CCNNNNNNNGG 8 cut(s) 94, 489, 593, 825, 839, 912, 950, 1054
BsmFI GGGAC 1 cut(s) 1375
BsnI GGCC 6 cut(s) 238, 865, 929, 1065, 1150, 1248
Bsp119I TTCGAA 1 cut(s) 476
Bsp1286I GDGCHC 1 cut(s) 1047
Bsp13I TCCGGA 2 cut(s) 508, 943
Bsp1407I TGTACA 1 cut(s) 672
Bsp143I GATC 6 cut(s) 498, 611, 982, 1334, 1338, 1369
BspANI GGCC 6 cut(s) 238, 865, 929, 1065, 1150, 1248
BspCNI CTCAG 3 cut(s) 66, 600, 1355
BspDI ATCGAT 1 cut(s) 1337
BspEI TCCGGA 2 cut(s) 508, 943
BspLI GGNNCC 3 cut(s) 282, 291, 831
BspMAI CTGCAG 1 cut(s) 738
BspPI GGATC 1 cut(s) 1342
BspQI GCTCTTC 1 cut(s) 1172
BspT104I TTCGAA 1 cut(s) 476
BspT107I GGYRCC 2 cut(s) 280, 829
BsrBI CCGCTC 2 cut(s) 108, 235
BsrDI GCAATG 2 cut(s) 967, 1045
BsrFI RCCGGY 3 cut(s) 196, 283, 537
BsrGI TGTACA 1 cut(s) 672
BsrI ACTGG 3 cut(s) 180, 598, 1483
BssAI RCCGGY 3 cut(s) 196, 283, 537
BssECI CCNNGG 2 cut(s) 879, 1145
BssMI GATC 6 cut(s) 498, 611, 982, 1334, 1338, 1369
BssNI GRCGYC 2 cut(s) 281, 830
BssT1I CCWWGG 2 cut(s) 879, 1145
Bst2UI CCWGG 1 cut(s) 1332
Bst4CI ACNGT 1 cut(s) 691
Bst6I CTCTTC 3 cut(s) 275, 392, 1172
BstACI GRCGYC 2 cut(s) 281, 830
BstAUI TGTACA 1 cut(s) 672
BstBI TTCGAA 1 cut(s) 476
BstC8I GCNNGC 4 cut(s) 92, 198, 285, 1184
BstDEI CTNAG 4 cut(s) 53, 608, 1152, 1342
BstF5I GGATG 7 cut(s) 424, 667, 721, 745, 807, 1011, 1084
BstH2I RGCGCY 3 cut(s) 284, 734, 833
BstHHI GCGC 4 cut(s) 196, 283, 733, 832
BstKTI GATC 6 cut(s) 501, 614, 985, 1337, 1341, 1372
BstMBI GATC 6 cut(s) 498, 611, 982, 1334, 1338, 1369
BstMCI CGRYCG 1 cut(s) 239
BstMWI GCNNNNNNNGC 3 cut(s) 111, 871, 1447
BstNI CCWGG 1 cut(s) 1332
BstNSI RCATGY 1 cut(s) 1269
BstSCI CCNGG 1 cut(s) 1330
BstSFI CTRYAG 1 cut(s) 734
BstV1I GCAGC 3 cut(s) 475, 720, 1453
BstV2I GAAGAC 1 cut(s) 369
BstZI CGGCCG 1 cut(s) 236
Bsu15I ATCGAT 1 cut(s) 1337
Bsu36I CCTNAGG 1 cut(s) 1152
BsuRI GGCC 6 cut(s) 238, 865, 929, 1065, 1150, 1248
BsuTUI ATCGAT 1 cut(s) 1337
BtrI CACGTC 1 cut(s) 361
BtsCI GGATG 7 cut(s) 424, 667, 721, 745, 807, 1011, 1084
BtsIMutI CAGTG 2 cut(s) 187, 1350
Cac8I GCNNGC 4 cut(s) 92, 198, 285, 1184
CfoI GCGC 4 cut(s) 196, 283, 733, 832
Cfr10I RCCGGY 3 cut(s) 196, 283, 537
Cfr13I GGNCC 1 cut(s) 876
ClaI ATCGAT 1 cut(s) 1337
Csp6I GTAC 4 cut(s) 206, 673, 838, 1273
CviAII CATG 2 cut(s) 191, 1266
CviQI GTAC 4 cut(s) 206, 673, 838, 1273
DdeI CTNAG 4 cut(s) 53, 608, 1152, 1342
DinI GGCGCC 2 cut(s) 282, 831
DpnI GATC 6 cut(s) 500, 613, 984, 1336, 1340, 1371
DpnII GATC 6 cut(s) 498, 611, 982, 1334, 1338, 1369
EaeI YGGCCR 2 cut(s) 236, 863
EagI CGGCCG 1 cut(s) 236
Eam1104I CTCTTC 3 cut(s) 275, 392, 1172
EarI CTCTTC 3 cut(s) 275, 392, 1172
EciI GGCGGA 3 cut(s) 59, 674, 764
EclXI CGGCCG 1 cut(s) 236
Eco130I CCWWGG 2 cut(s) 879, 1145
Eco147I AGGCCT 2 cut(s) 1065, 1150
Eco24I GRGCYC 1 cut(s) 1047
Eco47I GGWCC 1 cut(s) 876
Eco52I CGGCCG 1 cut(s) 236
Eco57I CTGAAG 2 cut(s) 944, 1466
Eco81I CCTNAGG 1 cut(s) 1152
EcoRI GAATTC 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 1330
EcoT14I CCWWGG 2 cut(s) 879, 1145
EcoT38I GRGCYC 1 cut(s) 1047
EgeI GGCGCC 2 cut(s) 282, 831
EheI GGCGCC 2 cut(s) 282, 831
ErhI CCWWGG 2 cut(s) 879, 1145
FaeI CATG 2 cut(s) 194, 1269
FaqI GGGAC 1 cut(s) 1375
FatI CATG 2 cut(s) 190, 1265
FauI CCCGC 2 cut(s) 87, 119
FokI GGATG 7 cut(s) 411, 674, 728, 752, 814, 1018, 1091
FriOI GRGCYC 1 cut(s) 1047
GlaI GCGC 4 cut(s) 195, 282, 732, 831
GsaI CCCAGC 1 cut(s) 829
GsuI CTGGAG 3 cut(s) 136, 1021, 1080
HaeII RGCGCY 3 cut(s) 284, 734, 833
HaeIII GGCC 6 cut(s) 238, 865, 929, 1065, 1150, 1248
HapII CCGG 7 cut(s) 197, 284, 509, 538, 587, 833, 944
HhaI GCGC 4 cut(s) 196, 283, 733, 832
Hin1I GRCGYC 2 cut(s) 281, 830
Hin1II CATG 2 cut(s) 194, 1269
Hin6I GCGC 4 cut(s) 194, 281, 731, 830
HinP1I GCGC 4 cut(s) 194, 281, 731, 830
HincII GTYRAC 1 cut(s) 961
HindII GTYRAC 1 cut(s) 961
HinfI GANTC 6 cut(s) 241, 349, 415, 451, 1082, 1223
HpaII CCGG 7 cut(s) 197, 284, 509, 538, 587, 833, 944
HphI GGTGA 2 cut(s) 1305, 1427
Hpy166II GTNNAC 3 cut(s) 208, 961, 1142
Hpy8I GTNNAC 3 cut(s) 208, 961, 1142
Hpy99I CGWCG 2 cut(s) 619, 658
HpyAV CCTTC 6 cut(s) 551, 739, 754, 919, 1205, 1431
HpyCH4III ACNGT 1 cut(s) 691
HpyCH4IV ACGT 2 cut(s) 360, 391
HpyCH4V TGCA 7 cut(s) 424, 736, 972, 1199, 1258, 1269, 1307
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 871, 1447
HpyF3I CTNAG 4 cut(s) 53, 608, 1152, 1342
HpySE526I ACGT 2 cut(s) 360, 391
Hsp92I GRCGYC 2 cut(s) 281, 830
Hsp92II CATG 2 cut(s) 194, 1269
HspAI GCGC 4 cut(s) 194, 281, 731, 830
KasI GGCGCC 2 cut(s) 280, 829
Kpn2I TCCGGA 2 cut(s) 508, 943
KroI GCCGGC 2 cut(s) 196, 283
KroNI GCCGGC 2 cut(s) 198, 285
Kzo9I GATC 6 cut(s) 498, 611, 982, 1334, 1338, 1369
LguI GCTCTTC 1 cut(s) 1172
LmnI GCTCC 4 cut(s) 69, 83, 113, 155
Lsp1109I GCAGC 3 cut(s) 475, 720, 1453
LweI GCATC 5 cut(s) 433, 565, 706, 1186, 1310
MaeII ACGT 2 cut(s) 360, 391
MaeIII GTNAC 2 cut(s) 589, 1456
MalI GATC 6 cut(s) 500, 613, 984, 1336, 1340, 1371
MbiI CCGCTC 2 cut(s) 108, 235
MboI GATC 6 cut(s) 498, 611, 982, 1334, 1338, 1369
MfeI CAATTG 1 cut(s) 542
MhlI GDGCHC 1 cut(s) 1047
MluCI AATT 6 cut(s) 305, 430, 542, 1069, 1322, 1408
Mly113I GGCGCC 2 cut(s) 281, 830
MlyI GAGTC 3 cut(s) 424, 1091, 1232
MreI CGCCGGCG 2 cut(s) 196, 283
MroI TCCGGA 2 cut(s) 508, 943
MroNI GCCGGC 2 cut(s) 196, 283
MroXI GAANNNNTTC 1 cut(s) 893
MseI TTAA 4 cut(s) 224, 488, 870, 1017
MslI CAYNNNNRTG 3 cut(s) 38, 990, 1513
MspA1I CMGCKG 3 cut(s) 90, 775, 1444
MspI CCGG 7 cut(s) 197, 284, 509, 538, 587, 833, 944
MspR9I CCNGG 1 cut(s) 1332
MunI CAATTG 1 cut(s) 542
MvaI CCWGG 1 cut(s) 1332
MwoI GCNNNNNNNGC 3 cut(s) 111, 871, 1447
NaeI GCCGGC 2 cut(s) 198, 285
NarI GGCGCC 2 cut(s) 281, 830
NdeII GATC 6 cut(s) 498, 611, 982, 1334, 1338, 1369
NgoMIV GCCGGC 2 cut(s) 196, 283
NlaIII CATG 2 cut(s) 194, 1269
NlaIV GGNNCC 3 cut(s) 282, 291, 831
NmeAIII GCCGAG 2 cut(s) 325, 844
NspI RCATGY 1 cut(s) 1269
NspV TTCGAA 1 cut(s) 476
PceI AGGCCT 2 cut(s) 1065, 1150
PciSI GCTCTTC 1 cut(s) 1172
PcsI WCGNNNNNNNCGW 2 cut(s) 863, 903
PdiI GCCGGC 2 cut(s) 198, 285
PdmI GAANNNNTTC 1 cut(s) 893
PfeI GAWTC 3 cut(s) 241, 349, 451
PflMI CCANNNNNTGG 1 cut(s) 1054
PfoI TCCNGGA 1 cut(s) 1330
PleI GAGTC 3 cut(s) 423, 1090, 1231
PluTI GGCGCC 2 cut(s) 284, 833
PpsI GAGTC 3 cut(s) 423, 1090, 1231
PshBI ATTAAT 1 cut(s) 1017
Psp6I CCWGG 1 cut(s) 1330
PspFI CCCAGC 1 cut(s) 825
PspGI CCWGG 1 cut(s) 1330
PspN4I GGNNCC 3 cut(s) 282, 291, 831
PspPI GGNCC 1 cut(s) 876
PstI CTGCAG 1 cut(s) 738
PvuII CAGCTG 1 cut(s) 1444
RsaI GTAC 4 cut(s) 207, 674, 839, 1274
RsaNI GTAC 4 cut(s) 206, 673, 838, 1273
RseI CAYNNNNRTG 3 cut(s) 38, 990, 1513
SapI GCTCTTC 1 cut(s) 1172
SaqAI TTAA 4 cut(s) 224, 488, 870, 1017
Sau3AI GATC 6 cut(s) 498, 611, 982, 1334, 1338, 1369
Sau96I GGNCC 1 cut(s) 876
SchI GAGTC 3 cut(s) 424, 1091, 1232
ScrFI CCNGG 1 cut(s) 1332
SduI GDGCHC 1 cut(s) 1047
SfaNI GCATC 5 cut(s) 433, 565, 706, 1186, 1310
SfcI CTRYAG 1 cut(s) 734
SfoI GGCGCC 2 cut(s) 282, 831
SfuI TTCGAA 1 cut(s) 476
SgrAI CRCCGGYG 2 cut(s) 196, 283
SinI GGWCC 1 cut(s) 876
SmiMI CAYNNNNRTG 3 cut(s) 38, 990, 1513
SmlI CTYRAG 1 cut(s) 1095
SmoI CTYRAG 1 cut(s) 1095
Sse9I AATT 6 cut(s) 305, 430, 542, 1069, 1322, 1408
SseBI AGGCCT 2 cut(s) 1065, 1150
SspDI GGCGCC 2 cut(s) 280, 829
StuI AGGCCT 2 cut(s) 1065, 1150
StyD4I CCNGG 1 cut(s) 1330
StyI CCWWGG 2 cut(s) 879, 1145
TaaI ACNGT 1 cut(s) 691
TaiI ACGT 2 cut(s) 363, 394
TasI AATT 6 cut(s) 305, 430, 542, 1069, 1322, 1408
TatI WGTACW 2 cut(s) 205, 672
TauI GCSGC 7 cut(s) 105, 108, 238, 266, 775, 791, 794
TfiI GAWTC 3 cut(s) 241, 349, 451
Tru1I TTAA 4 cut(s) 224, 488, 870, 1017
Tru9I TTAA 4 cut(s) 224, 488, 870, 1017
TscAI CASTG 2 cut(s) 187, 1350
TseI GCWGC 3 cut(s) 463, 733, 1441
TspDTI ATGAA 5 cut(s) 189, 341, 452, 782, 818
TspGWI ACGGA 1 cut(s) 1290
TspRI CASTG 2 cut(s) 187, 1350
Van91I CCANNNNNTGG 1 cut(s) 1054
VpaK11BI GGWCC 1 cut(s) 876
VspI ATTAAT 1 cut(s) 1017
XapI RAATTY 3 cut(s) 305, 430, 1322
XceI RCATGY 1 cut(s) 1269
XcmI CCANNNNNNNNNTGG 1 cut(s) 1055
XmnI GAANNNNTTC 1 cut(s) 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.