RLG00000012068

Endonuclease/Exonuclease/phosphatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010396
Physical Location & Seq
Reverse (-)
14970611 .. 14973338
2728 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012068

Sequence Viewer

Length: 1497 bp
ATGCTCAGAAACCTCCGCCGCCTCTGCTCCCGTCTCCGGTGGCCGTCCCGCCGCCGGTGCCGGAACAAAGTCGTCATCAAACGGTTCTGTAAAACGAACTCCAAGTCCCAATTCGAGCACGAACCCACTTCCAACGGGTCGTCGTCGGCGACGGTTCACCCGAACGGCCAGCTGGGCGGGTCGGAAACTGAGAAGTCCAGGCCTATTCGGGTCGCCACTTTCAACGCCGCCCTGTTCTCAATGGCGCCGGCGATTCCGACGCCGGAGAAAGTCGAGCAAGACGAAAATGGGGTCAAGGTCGTAAAGAACATAAATTCCAGAGCCAAATCGGTGACTGACAGACCCAAGAGTATCTTAAAGCGGTCGCCTTTACACCCAAATTTGGTGAACAACTCAGAAAATCAAAAAACCCAACAGAAATTCGTCAAATCCAAGTTGAGGGTATCGATCAATTTGCCGGATAATGAGATTTCATTACTGAAAAGCAGACAGTTGAGCTTTGCTGAAGGGAGAGAAGTTGATGATTCTTCATCTTCTTTTTCCCCAAGTAGAGTTTCAAGAGGAAAAGGTCCATTGAAGCCTAGTTTGAGCTTTCCTAATAATATCGGGAACGGTGGTGGTGGTGGTGATAATGGAGATTACTGTAGAAGCCACAGAACTGTTCTTGAGGTTCTGAGAGAATTGGATGCTGACATTTTGGCTCTGCAAGATGTGAAAGCAGAGGAGGAGAAGGAAATGAAGCCTCTTTCCGATTTGGCCGCCGCTTTGGGGATGAACTATGTCTTCGCCGAGAGTTGGGCGCCGGAGTACGGCAACGCCATCTTGTCAAAATGGCCGATAAAGCGGTCTAAGGTTCAGAAAATCTTTGATGACACTGATTTCAGGAACGTTCTAAAAGCCACCATTGACGTACCCCAAGCCGGAGAAGTCAACTTCCACTGCACCCACCTTGATCATCTTGATGAGAACTGGAGGATGAGGCAGATTAACTCTATAATCCAATCGAGCAACGAGCCTCACATATTGGCTGGAGGTCTAAATTCACTAGAGGAATCGGATTATTCTCAAGAAAGATGGACTGACATTGTAAAGTATTATGAGGAGATGGGAAAACCAACACCTAAGGTCGAAGTAATGAGATACTTGAAGAGCAAGCAGTATACAGATGCCAAGGATTTTGAAGGAGAATATGAGTCGGTAGTTATGATTGCCAAAGGCCAAAGCGTGCAGGGGACGTGCAAGTATGGAACACGGGTAGATTACATATTGGCGTCCTCAAATTCGCCCTACAAGTTTGTTCCCGGATCATACTCTGTCTTCTCATCCAAAGGGACATCTGATCATCATATAGTAAGAGTTGATGTGATCAAATCGGATAGTGGTTGTGATCCAGGAAATGTCTCCAGACGAGGGCAACGGAAACAGAAAGTTATAAAGATATCGGAGACTACTCCATCAAAAGGCATATGGAAAACAGAGATAAGATCAGAAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

499

Amino Acids

55.86

Weight (kDa)

9.39

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 209 - 448 7.7e-13 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1433
AccB1I GGYRCC 3 cut(s) 57, 244, 799
AccI GTMKAC 1 cut(s) 1160
AclI AACGTT 1 cut(s) 888
AclWI GGATC 2 cut(s) 1312, 1382
AcoI YGGCCR 4 cut(s) 41, 166, 756, 833
AcsI RAATTY 5 cut(s) 313, 379, 419, 1039, 1279
AcuI CTGAAG 1 cut(s) 525
AcyI GRCGYC 4 cut(s) 245, 260, 800, 1271
AfaI GTAC 2 cut(s) 809, 912
AgsI TTSAA 5 cut(s) 223, 558, 577, 1147, 1181
AjiI CACGTC 1 cut(s) 1236
AjnI CCWGG 2 cut(s) 197, 1390
AjuI GAANNNNNNNTTGG 4 cut(s) 95, 127, 538, 570
AloI GAACNNNNNNTCC 4 cut(s) 89, 121, 299, 331
AluBI AGCT 3 cut(s) 172, 498, 591
AluI AGCT 3 cut(s) 172, 498, 591
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 3 cut(s) 38, 1405, 1439
AlwI GGATC 2 cut(s) 1312, 1382
AoxI GGCC 6 cut(s) 41, 166, 200, 756, 833, 1216
ApoI RAATTY 5 cut(s) 313, 379, 419, 1039, 1279
ArsI GACNNNNNNTTYG 4 cut(s) 89, 121, 276, 308
AspLEI GCGC 2 cut(s) 247, 802
AspS9I GGNCC 1 cut(s) 569
AsuC2I CCSGG 1 cut(s) 1302
AsuHPI GGTGA 4 cut(s) 149, 343, 397, 638
AvaII GGWCC 1 cut(s) 569
AxyI CCTNAGG 1 cut(s) 1122
BanI GGYRCC 3 cut(s) 57, 244, 799
BbsI GAAGAC 2 cut(s) 775, 1309
Bbv12I GWGCWC 1 cut(s) 120
BccI CCATC 4 cut(s) 827, 1068, 1099, 1462
BceAI ACGGC 3 cut(s) 28, 181, 826
BcgI CGANNNNNNTGC 2 cut(s) 436, 470
BciT130I CCWGG 2 cut(s) 199, 1392
BclI TGATCA 3 cut(s) 952, 1339, 1365
BcnI CCSGG 1 cut(s) 1302
BcoDI GTCTC 3 cut(s) 38, 1405, 1439
BfaI CTAG 2 cut(s) 582, 1046
BfmI CTRYAG 1 cut(s) 643
BfoI RGCGCY 2 cut(s) 248, 803
BglI GCCNNNNNGGC 1 cut(s) 174
BisI GCNGC 5 cut(s) 19, 52, 228, 759, 762
BlsI GCNGC 5 cut(s) 20, 53, 229, 760, 763
Bme1390I CCNGG 3 cut(s) 199, 1302, 1392
Bme18I GGWCC 1 cut(s) 569
BmgBI CACGTC 1 cut(s) 1236
BmgT120I GGNCC 1 cut(s) 569
BmiI GGNNCC 3 cut(s) 59, 246, 801
BmrFI CCNGG 3 cut(s) 199, 1302, 1392
BmsI GCATC 2 cut(s) 676, 1156
BpiI GAAGAC 2 cut(s) 775, 1309
BpmI CTGGAG 3 cut(s) 991, 1050, 1387
BpuEI CTTGAG 2 cut(s) 686, 1050
BpuMI CCSGG 1 cut(s) 1302
Bsa29I ATCGAT 1 cut(s) 446
BsaHI GRCGYC 4 cut(s) 245, 260, 800, 1271
BsaJI CCNNGG 1 cut(s) 1170
BsaWI WCCGGW 1 cut(s) 36
Bse118I RCCGGY 2 cut(s) 54, 247
Bse1I ACTGG 1 cut(s) 974
Bse21I CCTNAGG 1 cut(s) 1122
BseBI CCWGG 2 cut(s) 199, 1392
BseCI ATCGAT 1 cut(s) 446
BseDI CCNNGG 1 cut(s) 1170
BseGI GGATG 4 cut(s) 691, 777, 981, 1322
BseMII CTCAG 4 cut(s) 19, 180, 408, 665
BseNI ACTGG 1 cut(s) 974
BseRI GAGGAG 3 cut(s) 737, 740, 1115
BseYI CCCAGC 1 cut(s) 172
BsgI GTGCAG 2 cut(s) 925, 1247
Bsh1285I CGRYCG 1 cut(s) 365
BshFI GGCC 6 cut(s) 43, 168, 202, 758, 835, 1218
BshNI GGYRCC 3 cut(s) 57, 244, 799
BshVI ATCGAT 1 cut(s) 446
BsiEI CGRYCG 1 cut(s) 365
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 9 cut(s) 37, 55, 61, 248, 263, 458, 803, 921, 1302
BslFI GGGAC 4 cut(s) 31, 91, 1246, 1345
BsmAI GTCTC 3 cut(s) 38, 1405, 1439
BsmBI CGTCTC 1 cut(s) 38
BsmFI GGGAC 4 cut(s) 31, 91, 1246, 1345
BsnI GGCC 6 cut(s) 43, 168, 202, 758, 835, 1218
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 7 cut(s) 447, 952, 1304, 1339, 1365, 1387, 1484
BspANI GGCC 6 cut(s) 43, 168, 202, 758, 835, 1218
BspCNI CTCAG 4 cut(s) 18, 181, 407, 666
BspDI ATCGAT 1 cut(s) 446
BspLI GGNNCC 3 cut(s) 59, 246, 801
BspPI GGATC 2 cut(s) 1312, 1382
BspQI GCTCTTC 1 cut(s) 1142
BspT107I GGYRCC 3 cut(s) 57, 244, 799
BsrFI RCCGGY 2 cut(s) 54, 247
BsrI ACTGG 1 cut(s) 974
BssAI RCCGGY 2 cut(s) 54, 247
BssECI CCNNGG 1 cut(s) 1170
BssMI GATC 7 cut(s) 447, 952, 1304, 1339, 1365, 1387, 1484
BssNAI GTATAC 1 cut(s) 1161
BssNI GRCGYC 4 cut(s) 245, 260, 800, 1271
BssT1I CCWWGG 1 cut(s) 1170
Bst1107I GTATAC 1 cut(s) 1161
Bst2UI CCWGG 2 cut(s) 199, 1392
Bst4CI ACNGT 6 cut(s) 84, 154, 492, 614, 644, 661
Bst6I CTCTTC 1 cut(s) 1142
BstACI GRCGYC 4 cut(s) 245, 260, 800, 1271
BstC8I GCNNGC 4 cut(s) 170, 249, 1154, 1226
BstDEI CTNAG 6 cut(s) 5, 189, 394, 674, 849, 1122
BstF5I GGATG 4 cut(s) 691, 777, 981, 1322
BstH2I RGCGCY 2 cut(s) 248, 803
BstHHI GCGC 2 cut(s) 247, 802
BstKTI GATC 7 cut(s) 450, 955, 1307, 1342, 1368, 1390, 1487
BstMAI GTCTC 3 cut(s) 38, 1405, 1439
BstMBI GATC 7 cut(s) 447, 952, 1304, 1339, 1365, 1387, 1484
BstMCI CGRYCG 1 cut(s) 365
BstMWI GCNNNNNNNGC 4 cut(s) 24, 57, 174, 841
BstNI CCWGG 2 cut(s) 199, 1392
BstSCI CCNGG 3 cut(s) 197, 1300, 1390
BstSFI CTRYAG 1 cut(s) 643
BstV2I GAAGAC 2 cut(s) 775, 1309
BstZ17I GTATAC 1 cut(s) 1161
Bsu15I ATCGAT 1 cut(s) 446
Bsu36I CCTNAGG 1 cut(s) 1122
BsuRI GGCC 6 cut(s) 43, 168, 202, 758, 835, 1218
BsuTUI ATCGAT 1 cut(s) 446
BtrI CACGTC 1 cut(s) 1236
BtsCI GGATG 4 cut(s) 691, 777, 981, 1322
BtsI GCAGTG 1 cut(s) 937
BtsIMutI CAGTG 2 cut(s) 873, 937
Cac8I GCNNGC 4 cut(s) 170, 249, 1154, 1226
CfoI GCGC 2 cut(s) 247, 802
Cfr10I RCCGGY 2 cut(s) 54, 247
Cfr13I GGNCC 1 cut(s) 569
ClaI ATCGAT 1 cut(s) 446
CseI GACGC 2 cut(s) 268, 1260
Csp6I GTAC 2 cut(s) 808, 911
CviQI GTAC 2 cut(s) 808, 911
DdeI CTNAG 6 cut(s) 5, 189, 394, 674, 849, 1122
DinI GGCGCC 2 cut(s) 246, 801
DpnI GATC 7 cut(s) 449, 954, 1306, 1341, 1367, 1389, 1486
DpnII GATC 7 cut(s) 447, 952, 1304, 1339, 1365, 1387, 1484
EaeI YGGCCR 4 cut(s) 41, 166, 756, 833
Eam1104I CTCTTC 1 cut(s) 1142
EarI CTCTTC 1 cut(s) 1142
EciI GGCGGA 1 cut(s) 5
Eco130I CCWWGG 1 cut(s) 1170
Eco147I AGGCCT 1 cut(s) 202
Eco32I GATATC 1 cut(s) 1440
Eco47I GGWCC 1 cut(s) 569
Eco57I CTGAAG 1 cut(s) 525
Eco81I CCTNAGG 1 cut(s) 1122
EcoRII CCWGG 2 cut(s) 197, 1390
EcoRV GATATC 1 cut(s) 1440
EcoT14I CCWWGG 1 cut(s) 1170
EgeI GGCGCC 2 cut(s) 246, 801
EheI GGCGCC 2 cut(s) 246, 801
ErhI CCWWGG 1 cut(s) 1170
Esp3I CGTCTC 1 cut(s) 38
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 4 cut(s) 31, 91, 1246, 1345
FauI CCCGC 2 cut(s) 56, 170
FauNDI CATATG 1 cut(s) 1466
FbaI TGATCA 3 cut(s) 952, 1339, 1365
FblI GTMKAC 1 cut(s) 1160
Fnu4HI GCNGC 5 cut(s) 19, 52, 228, 759, 762
FokI GGATG 4 cut(s) 698, 784, 988, 1309
Fsp4HI GCNGC 5 cut(s) 19, 52, 228, 759, 762
FspBI CTAG 2 cut(s) 582, 1046
GlaI GCGC 2 cut(s) 246, 801
GluI GCNGC 5 cut(s) 19, 52, 228, 759, 762
GsaI CCCAGC 1 cut(s) 176
GsuI CTGGAG 3 cut(s) 991, 1050, 1387
HaeII RGCGCY 2 cut(s) 248, 803
HaeIII GGCC 6 cut(s) 43, 168, 202, 758, 835, 1218
HapII CCGG 9 cut(s) 37, 55, 61, 248, 263, 458, 803, 921, 1302
HgaI GACGC 2 cut(s) 268, 1260
HhaI GCGC 2 cut(s) 247, 802
Hin1I GRCGYC 4 cut(s) 245, 260, 800, 1271
Hin6I GCGC 2 cut(s) 245, 800
HinP1I GCGC 2 cut(s) 245, 800
HincII GTYRAC 1 cut(s) 931
HindII GTYRAC 1 cut(s) 931
HinfI GANTC 4 cut(s) 253, 524, 1052, 1193
HpaII CCGG 9 cut(s) 37, 55, 61, 248, 263, 458, 803, 921, 1302
HphI GGTGA 4 cut(s) 149, 343, 397, 638
Hpy166II GTNNAC 4 cut(s) 157, 388, 931, 1161
Hpy188III TCNNGA 8 cut(s) 318, 558, 607, 665, 883, 959, 1067, 1404
Hpy8I GTNNAC 4 cut(s) 157, 388, 931, 1161
Hpy99I CGWCG 4 cut(s) 145, 148, 154, 262
HpyAV CCTTC 4 cut(s) 500, 724, 1175, 1484
HpyCH4III ACNGT 6 cut(s) 84, 154, 492, 614, 644, 661
HpyCH4IV ACGT 3 cut(s) 888, 909, 1235
HpyCH4V TGCA 4 cut(s) 706, 942, 1228, 1239
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 57, 174, 841
HpyF3I CTNAG 6 cut(s) 5, 189, 394, 674, 849, 1122
HpySE526I ACGT 3 cut(s) 888, 909, 1235
Hsp92I GRCGYC 4 cut(s) 245, 260, 800, 1271
HspAI GCGC 2 cut(s) 245, 800
KasI GGCGCC 2 cut(s) 244, 799
KroI GCCGGC 1 cut(s) 247
KroNI GCCGGC 1 cut(s) 249
Ksp22I TGATCA 3 cut(s) 952, 1339, 1365
Kzo9I GATC 7 cut(s) 447, 952, 1304, 1339, 1365, 1387, 1484
LguI GCTCTTC 1 cut(s) 1142
LmnI GCTCC 1 cut(s) 32
LweI GCATC 2 cut(s) 676, 1156
MaeI CTAG 2 cut(s) 582, 1046
MaeII ACGT 3 cut(s) 888, 909, 1235
MaeIII GTNAC 1 cut(s) 331
MalI GATC 7 cut(s) 449, 954, 1306, 1341, 1367, 1389, 1486
MboI GATC 7 cut(s) 447, 952, 1304, 1339, 1365, 1387, 1484
MboII GAAGA 5 cut(s) 519, 525, 775, 1159, 1309
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 8 cut(s) 110, 313, 379, 419, 451, 680, 1039, 1279
Mly113I GGCGCC 2 cut(s) 245, 800
MlyI GAGTC 1 cut(s) 1202
MmeI TCCRAC 3 cut(s) 156, 162, 281
MreI CGCCGGCG 1 cut(s) 247
MroNI GCCGGC 1 cut(s) 247
MseI TTAA 2 cut(s) 356, 987
MslI CAYNNNNRTG 1 cut(s) 960
MspA1I CMGCKG 1 cut(s) 172
MspI CCGG 9 cut(s) 37, 55, 61, 248, 263, 458, 803, 921, 1302
MspR9I CCNGG 3 cut(s) 199, 1302, 1392
MvaI CCWGG 2 cut(s) 199, 1392
MwoI GCNNNNNNNGC 4 cut(s) 24, 57, 174, 841
NaeI GCCGGC 1 cut(s) 249
NarI GGCGCC 2 cut(s) 245, 800
NciI CCSGG 1 cut(s) 1302
NdeI CATATG 1 cut(s) 1466
NdeII GATC 7 cut(s) 447, 952, 1304, 1339, 1365, 1387, 1484
NgoMIV GCCGGC 1 cut(s) 247
NlaIV GGNNCC 3 cut(s) 59, 246, 801
NmeAIII GCCGAG 1 cut(s) 814
NmuCI GTSAC 1 cut(s) 331
PceI AGGCCT 1 cut(s) 202
PciSI GCTCTTC 1 cut(s) 1142
PcsI WCGNNNNNNNCGW 3 cut(s) 146, 158, 279
PdiI GCCGGC 1 cut(s) 249
PfeI GAWTC 3 cut(s) 253, 524, 1052
PfoI TCCNGGA 2 cut(s) 1300, 1390
PkrI GCNGC 5 cut(s) 20, 53, 229, 760, 763
PleI GAGTC 1 cut(s) 1201
PluTI GGCGCC 2 cut(s) 248, 803
PpsI GAGTC 1 cut(s) 1201
PsiI TTATAA 1 cut(s) 1433
Psp1406I AACGTT 1 cut(s) 888
Psp6I CCWGG 2 cut(s) 197, 1390
PspFI CCCAGC 1 cut(s) 172
PspGI CCWGG 2 cut(s) 197, 1390
PspN4I GGNNCC 3 cut(s) 59, 246, 801
PspPI GGNCC 1 cut(s) 569
PvuII CAGCTG 1 cut(s) 172
RsaI GTAC 2 cut(s) 809, 912
RsaNI GTAC 2 cut(s) 808, 911
RseI CAYNNNNRTG 1 cut(s) 960
SapI GCTCTTC 1 cut(s) 1142
SaqAI TTAA 2 cut(s) 356, 987
SatI GCNGC 5 cut(s) 19, 52, 228, 759, 762
Sau3AI GATC 7 cut(s) 447, 952, 1304, 1339, 1365, 1387, 1484
Sau96I GGNCC 1 cut(s) 569
SchI GAGTC 1 cut(s) 1202
ScrFI CCNGG 3 cut(s) 199, 1302, 1392
SduI GDGCHC 1 cut(s) 120
SfaNI GCATC 2 cut(s) 676, 1156
SfcI CTRYAG 1 cut(s) 643
SfoI GGCGCC 2 cut(s) 246, 801
SgrAI CRCCGGYG 2 cut(s) 54, 247
SinI GGWCC 1 cut(s) 569
SmiMI CAYNNNNRTG 1 cut(s) 960
SmlI CTYRAG 2 cut(s) 665, 1065
SmoI CTYRAG 2 cut(s) 665, 1065
Sse9I AATT 8 cut(s) 110, 313, 379, 419, 451, 680, 1039, 1279
SseBI AGGCCT 1 cut(s) 202
SspDI GGCGCC 2 cut(s) 244, 799
SspMI CTAG 2 cut(s) 582, 1046
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 3 cut(s) 197, 1300, 1390
StyI CCWWGG 1 cut(s) 1170
TaaI ACNGT 6 cut(s) 84, 154, 492, 614, 644, 661
TaiI ACGT 3 cut(s) 891, 912, 1238
TaqI TCGA 5 cut(s) 114, 273, 446, 1004, 1128
TasI AATT 8 cut(s) 110, 313, 379, 419, 451, 680, 1039, 1279
TauI GCSGC 5 cut(s) 21, 54, 230, 761, 764
TfiI GAWTC 3 cut(s) 253, 524, 1052
Tru1I TTAA 2 cut(s) 356, 987
Tru9I TTAA 2 cut(s) 356, 987
TscAI CASTG 2 cut(s) 880, 944
TseFI GTSAC 1 cut(s) 331
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 4 cut(s) 462, 519, 752, 788
TspGWI ACGGA 1 cut(s) 1432
TspRI CASTG 2 cut(s) 880, 944
VpaK11BI GGWCC 1 cut(s) 569
XapI RAATTY 5 cut(s) 313, 379, 419, 1039, 1279
XmiI GTMKAC 1 cut(s) 1160
XspI CTAG 2 cut(s) 582, 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.