Rorug06G0223000

Endonuclease/Exonuclease/phosphatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010396
Physical Location & Seq
Forward (+)
35653000 .. 35654016
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0223000.1

Sequence Viewer

Length: 1017 bp
ATGGATAGGGAACAAGATGAGTTGCAGTTCCTTGGTCTCTTTGGCATCTACAAAGAATCCTATAAGATTGTATACAAATGGAGAAAAATCTTCAGCCAAATCAGCTTGACCTTGATCCTCCCTTTGACCTTCATCTTCTTGGCTCACATAGAAGTCTCCGACATCCTCTTCTCCAGGATCCTCCGGAGCGAATATGCCTTGGACGAAACGAGACCATACAGCCCTCTCTACAAGAGAACAAACGACCTCATCACCAAAGAATGGGTAACCTTCTGGCTCTTCAAGGCTGCTTACTTCACTGCCATGCTCATCTTCGGCCTCCTTTCCACCTCCGCCGTGGTATACACCATCGCATGCGTATACACCGCCCGGGAAATCACCTTCCGAAAGATCATGAGCGTCGTCCCAAGGGTCTGGAAGCGCGTGATGATCACCTTCTTGTGCACCTTCCTGGCTTTCTTCCTCTACAACCTAGTGGCAACAGTAGTCGTAATTGTTTGGTTAGCTCTCTTAAACAGCACACGCGGAGACGGCCACATCTTCGTCATAACTGGTATCTTTTTATTGATCTTGTACGCTGTTGGGTTCGTGTACGTGACCGTAATCTGGCAACTGGCTAGCGTCGTCTCGGTGTTGGAAGAAACGAAAGGGATCAAAGCCATGATCAAGAGCAAGAAGCTACTCAGAGGGAACATGTGGGTGGTCTCAATCATCTTCATCAAGATCATTGCTTTAGCTTACGTTATTCAGTTTTCATTTCAAGCATTGGTTGTGGATGGATGGGTGTTTGGGTCCTTTGGGATAATGGGCAGGATAGGCTTCGGTGTTCTCTTTTTGTTGTTGCTGCTTAAGTTGATTCTGTTTTTGCTGGTCATCCAAACGGTGCTTTATTTCGTCTGCAAATCGTACCACCATGAAAACATTGACAAGTCAGCTCTATCGGATCACCTCGAGGTTATTTATCTGGGAGACTATGTTCCTCTGAAATCTAAGGATGTTCAGCTTGAGCAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

338

Amino Acids

39.03

Weight (kDa)

9.29

Isoelectric Point (pI)

28.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 950
Acc36I ACCTGC 1 cut(s) 1000
AccB7I CCANNNNNTGG 1 cut(s) 261
AccI GTMKAC 3 cut(s) 72, 342, 360
AccII CGCG 2 cut(s) 423, 525
AccIII TCCGGA 1 cut(s) 183
AciI CCGC 3 cut(s) 333, 366, 525
AclWI GGATC 5 cut(s) 109, 172, 185, 659, 951
AcoI YGGCCR 1 cut(s) 532
AcuI CTGAAG 1 cut(s) 76
AfaI GTAC 3 cut(s) 575, 593, 908
AfiI CCNNNNNNNGG 2 cut(s) 261, 606
AflII CTTAAG 1 cut(s) 848
AflIII ACRYGT 1 cut(s) 693
AgsI TTSAA 2 cut(s) 283, 761
AjnI CCWGG 2 cut(s) 173, 450
AloI GAACNNNNNNTCC 2 cut(s) 960, 992
AluBI AGCT 6 cut(s) 105, 506, 679, 737, 935, 1003
AluI AGCT 6 cut(s) 105, 506, 679, 737, 935, 1003
Alw21I GWGCWC 1 cut(s) 446
Alw26I GTCTC 7 cut(s) 41, 160, 205, 522, 631, 709, 963
Alw44I GTGCAC 1 cut(s) 442
AlwI GGATC 5 cut(s) 109, 172, 185, 659, 951
Ama87I CYCGRG 2 cut(s) 369, 950
Aor13HI TCCGGA 1 cut(s) 183
AoxI GGCC 2 cut(s) 316, 532
ApaLI GTGCAC 1 cut(s) 442
ApeKI GCWGC 2 cut(s) 287, 844
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 1 cut(s) 792
AsuC2I CCSGG 2 cut(s) 370, 371
AsuHPI GGTGA 4 cut(s) 244, 370, 424, 938
AsuNHI GCTAGC 1 cut(s) 617
AvaI CYCGRG 2 cut(s) 369, 950
AvaII GGWCC 1 cut(s) 792
BaeGI GKGCMC 1 cut(s) 446
BamHI GGATCC 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 446
BbvI GCAGC 2 cut(s) 274, 831
BccI CCATC 3 cut(s) 356, 770, 774
BceAI ACGGC 2 cut(s) 320, 547
BciT130I CCWGG 2 cut(s) 175, 452
BclI TGATCA 2 cut(s) 429, 663
BcnI CCSGG 2 cut(s) 370, 371
BcoDI GTCTC 7 cut(s) 41, 160, 205, 522, 631, 709, 963
BfaI CTAG 2 cut(s) 473, 618
BfrI CTTAAG 1 cut(s) 848
BfuAI ACCTGC 1 cut(s) 1000
BisI GCNGC 2 cut(s) 288, 845
BlsI GCNGC 2 cut(s) 289, 846
Bme1390I CCNGG 4 cut(s) 175, 370, 371, 452
Bme18I GGWCC 1 cut(s) 792
BmeT110I CYCGRG 2 cut(s) 369, 950
BmgT120I GGNCC 1 cut(s) 792
BmiI GGNNCC 2 cut(s) 179, 793
BmrFI CCNGG 4 cut(s) 175, 370, 371, 452
BmsI GCATC 1 cut(s) 54
BmtI GCTAGC 1 cut(s) 621
BpmI CTGGAG 1 cut(s) 157
BpuMI CCSGG 2 cut(s) 370, 371
BsaAI YACGTR 1 cut(s) 595
BsaI GGTCTC 3 cut(s) 41, 205, 709
BsaJI CCNNGG 5 cut(s) 31, 198, 336, 369, 407
BsaWI WCCGGW 1 cut(s) 183
BsaXI ACNNNNNCTCC 2 cut(s) 960, 990
Bsc4I CCNNNNNNNGG 2 cut(s) 261, 606
Bse1I ACTGG 2 cut(s) 556, 618
Bse3DI GCAATG 1 cut(s) 726
BseAI TCCGGA 1 cut(s) 183
BseBI CCWGG 2 cut(s) 175, 452
BseDI CCNNGG 5 cut(s) 31, 198, 336, 369, 407
BseGI GGATG 5 cut(s) 162, 781, 785, 873, 1000
BseLI CCNNNNNNNGG 2 cut(s) 261, 606
BseMI GCAATG 1 cut(s) 726
BseMII CTCAG 1 cut(s) 697
BseNI ACTGG 2 cut(s) 556, 618
BseSI GKGCMC 1 cut(s) 446
BseXI GCAGC 2 cut(s) 274, 831
Bsh1236I CGCG 2 cut(s) 423, 525
BshFI GGCC 2 cut(s) 318, 534
BsiHKAI GWGCWC 1 cut(s) 446
BsiHKCI CYCGRG 2 cut(s) 369, 950
BsiSI CCGG 2 cut(s) 184, 370
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 2 cut(s) 261, 606
BsmAI GTCTC 7 cut(s) 41, 160, 205, 522, 631, 709, 963
BsmBI CGTCTC 2 cut(s) 522, 631
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 2 cut(s) 318, 534
Bso31I GGTCTC 3 cut(s) 41, 205, 709
BsoBI CYCGRG 2 cut(s) 369, 950
Bsp1286I GDGCHC 1 cut(s) 446
Bsp13I TCCGGA 1 cut(s) 183
Bsp143I GATC 9 cut(s) 114, 177, 390, 429, 567, 651, 663, 723, 943
BspACI CCGC 3 cut(s) 333, 366, 525
BspANI GGCC 2 cut(s) 318, 534
BspCNI CTCAG 1 cut(s) 696
BspEI TCCGGA 1 cut(s) 183
BspFNI CGCG 2 cut(s) 423, 525
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 2 cut(s) 179, 793
BspMI ACCTGC 1 cut(s) 1000
BspOI GCTAGC 1 cut(s) 621
BspPI GGATC 5 cut(s) 109, 172, 185, 659, 951
BspQI GCTCTTC 1 cut(s) 284
BspTI CTTAAG 1 cut(s) 848
BspTNI GGTCTC 3 cut(s) 41, 205, 709
BsrDI GCAATG 1 cut(s) 726
BsrI ACTGG 2 cut(s) 556, 618
BssECI CCNNGG 5 cut(s) 31, 198, 336, 369, 407
BssMI GATC 9 cut(s) 114, 177, 390, 429, 567, 651, 663, 723, 943
BssNAI GTATAC 3 cut(s) 73, 343, 361
BssT1I CCWWGG 3 cut(s) 31, 198, 407
Bst1107I GTATAC 3 cut(s) 73, 343, 361
Bst2UI CCWGG 2 cut(s) 175, 452
Bst4CI ACNGT 3 cut(s) 484, 601, 883
Bst6I CTCTTC 2 cut(s) 173, 284
BstAFI CTTAAG 1 cut(s) 848
BstBAI YACGTR 1 cut(s) 595
BstC8I GCNNGC 2 cut(s) 355, 619
BstDEI CTNAG 2 cut(s) 683, 990
BstDSI CCRYGG 1 cut(s) 336
BstEII GGTNACC 1 cut(s) 265
BstF5I GGATG 5 cut(s) 162, 781, 785, 873, 1000
BstFNI CGCG 2 cut(s) 423, 525
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 9 cut(s) 117, 180, 393, 432, 570, 654, 666, 726, 946
BstMAI GTCTC 7 cut(s) 41, 160, 205, 522, 631, 709, 963
BstMBI GATC 9 cut(s) 114, 177, 390, 429, 567, 651, 663, 723, 943
BstMWI GCNNNNNNNGC 3 cut(s) 102, 531, 816
BstNI CCWGG 2 cut(s) 175, 452
BstNSI RCATGY 2 cut(s) 357, 697
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 4 cut(s) 173, 368, 369, 450
BstSLI GKGCMC 1 cut(s) 446
BstUI CGCG 2 cut(s) 423, 525
BstV1I GCAGC 2 cut(s) 274, 831
BstX2I RGATCY 1 cut(s) 177
BstXI CCANNNNNNTGG 1 cut(s) 414
BstYI RGATCY 1 cut(s) 177
BstZ17I GTATAC 3 cut(s) 73, 343, 361
BsuRI GGCC 2 cut(s) 318, 534
BtgI CCRYGG 1 cut(s) 336
BtgZI GCGATG 1 cut(s) 334
BtsCI GGATG 5 cut(s) 162, 781, 785, 873, 1000
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 1 cut(s) 297
BveI ACCTGC 1 cut(s) 1000
Cac8I GCNNGC 2 cut(s) 355, 619
CciI TCATGA 1 cut(s) 393
CfoI GCGC 1 cut(s) 423
Cfr13I GGNCC 1 cut(s) 792
Cfr9I CCCGGG 1 cut(s) 369
CseI GACGC 2 cut(s) 388, 610
Csp6I GTAC 3 cut(s) 574, 592, 907
CviAII CATG 6 cut(s) 304, 354, 394, 661, 694, 914
CviQI GTAC 3 cut(s) 574, 592, 907
DdeI CTNAG 2 cut(s) 683, 990
DpnI GATC 9 cut(s) 116, 179, 392, 431, 569, 653, 665, 725, 945
DpnII GATC 9 cut(s) 114, 177, 390, 429, 567, 651, 663, 723, 943
EaeI YGGCCR 1 cut(s) 532
Eam1104I CTCTTC 2 cut(s) 173, 284
EarI CTCTTC 2 cut(s) 173, 284
EciI GGCGGA 1 cut(s) 322
Eco130I CCWWGG 3 cut(s) 31, 198, 407
Eco31I GGTCTC 3 cut(s) 41, 205, 709
Eco47I GGWCC 1 cut(s) 792
Eco57I CTGAAG 1 cut(s) 76
Eco88I CYCGRG 2 cut(s) 369, 950
Eco91I GGTNACC 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 792
EcoO65I GGTNACC 1 cut(s) 265
EcoRII CCWGG 2 cut(s) 173, 450
EcoT14I CCWWGG 3 cut(s) 31, 198, 407
ErhI CCWWGG 3 cut(s) 31, 198, 407
Esp3I CGTCTC 2 cut(s) 522, 631
FaeI CATG 6 cut(s) 307, 357, 397, 664, 697, 917
FaqI GGGAC 1 cut(s) 389
FatI CATG 6 cut(s) 303, 353, 393, 660, 693, 913
FbaI TGATCA 2 cut(s) 429, 663
FblI GTMKAC 3 cut(s) 72, 342, 360
Fnu4HI GCNGC 2 cut(s) 288, 845
FokI GGATG 5 cut(s) 149, 788, 792, 860, 1007
Fsp4HI GCNGC 2 cut(s) 288, 845
FspBI CTAG 2 cut(s) 473, 618
GlaI GCGC 1 cut(s) 422
GluI GCNGC 2 cut(s) 288, 845
GsuI CTGGAG 1 cut(s) 157
HaeIII GGCC 2 cut(s) 318, 534
HapII CCGG 2 cut(s) 184, 370
HgaI GACGC 2 cut(s) 388, 610
HhaI GCGC 1 cut(s) 423
Hin1II CATG 6 cut(s) 307, 357, 397, 664, 697, 917
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HinfI GANTC 2 cut(s) 56, 856
HpaII CCGG 2 cut(s) 184, 370
HphI GGTGA 4 cut(s) 244, 370, 424, 938
Hpy166II GTNNAC 5 cut(s) 73, 343, 361, 444, 592
Hpy188I TCNGA 5 cut(s) 160, 386, 686, 943, 984
Hpy188III TCNNGA 5 cut(s) 184, 394, 415, 667, 721
Hpy8I GTNNAC 5 cut(s) 73, 343, 361, 444, 592
Hpy99I CGWCG 2 cut(s) 404, 626
HpyAV CCTTC 5 cut(s) 139, 280, 391, 445, 457
HpyCH4III ACNGT 3 cut(s) 484, 601, 883
HpyCH4IV ACGT 2 cut(s) 594, 741
HpyCH4V TGCA 3 cut(s) 25, 444, 900
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 531, 816
HpyF3I CTNAG 2 cut(s) 683, 990
HpySE526I ACGT 2 cut(s) 594, 741
Hsp92II CATG 6 cut(s) 307, 357, 397, 664, 697, 917
HspAI GCGC 1 cut(s) 421
Kpn2I TCCGGA 1 cut(s) 183
Ksp22I TGATCA 2 cut(s) 429, 663
Kzo9I GATC 9 cut(s) 114, 177, 390, 429, 567, 651, 663, 723, 943
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 1 cut(s) 186
Lsp1109I GCAGC 2 cut(s) 274, 831
LweI GCATC 1 cut(s) 54
MaeI CTAG 2 cut(s) 473, 618
MaeII ACGT 2 cut(s) 594, 741
MaeIII GTNAC 2 cut(s) 265, 595
MalI GATC 9 cut(s) 116, 179, 392, 431, 569, 653, 665, 725, 945
MboI GATC 9 cut(s) 114, 177, 390, 429, 567, 651, 663, 723, 943
MboII GAAGA 9 cut(s) 82, 127, 160, 271, 304, 451, 532, 650, 706
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 446
MluCI AATT 1 cut(s) 492
MmeI TCCRAC 2 cut(s) 183, 615
MroI TCCGGA 1 cut(s) 183
MseI TTAA 3 cut(s) 512, 849, 1015
MslI CAYNNNNRTG 2 cut(s) 302, 698
MspCI CTTAAG 1 cut(s) 848
MspI CCGG 2 cut(s) 184, 370
MspR9I CCNGG 4 cut(s) 175, 370, 371, 452
MvaI CCWGG 2 cut(s) 175, 452
MvnI CGCG 2 cut(s) 423, 525
MwoI GCNNNNNNNGC 3 cut(s) 102, 531, 816
NciI CCSGG 2 cut(s) 370, 371
NdeII GATC 9 cut(s) 114, 177, 390, 429, 567, 651, 663, 723, 943
NheI GCTAGC 1 cut(s) 617
NlaIII CATG 6 cut(s) 307, 357, 397, 664, 697, 917
NlaIV GGNNCC 2 cut(s) 179, 793
NmuCI GTSAC 1 cut(s) 595
NspI RCATGY 2 cut(s) 357, 697
PaeI GCATGC 1 cut(s) 357
PaeR7I CTCGAG 1 cut(s) 950
PagI TCATGA 1 cut(s) 393
PciI ACATGT 1 cut(s) 693
PciSI GCTCTTC 1 cut(s) 284
PfeI GAWTC 2 cut(s) 56, 856
PflMI CCANNNNNTGG 1 cut(s) 261
PfoI TCCNGGA 1 cut(s) 173
PkrI GCNGC 2 cut(s) 289, 846
Ppu21I YACGTR 1 cut(s) 595
PpuMI RGGWCCY 1 cut(s) 792
PscI ACATGT 1 cut(s) 693
Psp5II RGGWCCY 1 cut(s) 792
Psp6I CCWGG 2 cut(s) 173, 450
PspEI GGTNACC 1 cut(s) 265
PspGI CCWGG 2 cut(s) 173, 450
PspN4I GGNNCC 2 cut(s) 179, 793
PspPI GGNCC 1 cut(s) 792
PspPPI RGGWCCY 1 cut(s) 792
PspXI VCTCGAGB 1 cut(s) 950
PsuI RGATCY 1 cut(s) 177
RsaI GTAC 3 cut(s) 575, 593, 908
RsaNI GTAC 3 cut(s) 574, 592, 907
RseI CAYNNNNRTG 2 cut(s) 302, 698
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 3 cut(s) 512, 849, 1015
SatI GCNGC 2 cut(s) 288, 845
Sau3AI GATC 9 cut(s) 114, 177, 390, 429, 567, 651, 663, 723, 943
Sau96I GGNCC 1 cut(s) 792
ScrFI CCNGG 4 cut(s) 175, 370, 371, 452
SduI GDGCHC 1 cut(s) 446
SfaNI GCATC 1 cut(s) 54
Sfr274I CTCGAG 1 cut(s) 950
SinI GGWCC 1 cut(s) 792
SlaI CTCGAG 1 cut(s) 950
SmaI CCCGGG 1 cut(s) 371
SmiMI CAYNNNNRTG 2 cut(s) 302, 698
SmlI CTYRAG 3 cut(s) 848, 950, 1004
SmoI CTYRAG 3 cut(s) 848, 950, 1004
SphI GCATGC 1 cut(s) 357
Sse9I AATT 1 cut(s) 492
SsiI CCGC 3 cut(s) 333, 366, 525
SspMI CTAG 2 cut(s) 473, 618
StyD4I CCNGG 4 cut(s) 173, 368, 369, 450
StyI CCWWGG 3 cut(s) 31, 198, 407
TaaI ACNGT 3 cut(s) 484, 601, 883
TaiI ACGT 2 cut(s) 597, 744
TaqI TCGA 1 cut(s) 951
TasI AATT 1 cut(s) 492
TfiI GAWTC 2 cut(s) 56, 856
Tru1I TTAA 3 cut(s) 512, 849, 1015
Tru9I TTAA 3 cut(s) 512, 849, 1015
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 595
TseI GCWGC 2 cut(s) 287, 844
Tsp45I GTSAC 1 cut(s) 595
TspDTI ATGAA 4 cut(s) 121, 706, 744, 930
TspMI CCCGGG 1 cut(s) 369
TspRI CASTG 1 cut(s) 304
Van91I CCANNNNNTGG 1 cut(s) 261
Vha464I CTTAAG 1 cut(s) 848
VneI GTGCAC 1 cut(s) 442
VpaK11BI GGWCC 1 cut(s) 792
XceI RCATGY 2 cut(s) 357, 697
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
XhoI CTCGAG 1 cut(s) 950
XmaI CCCGGG 1 cut(s) 369
XmiI GTMKAC 3 cut(s) 72, 342, 360
XspI CTAG 2 cut(s) 473, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.