Rw6G029290

Endonuclease/Exonuclease/phosphatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010396
Physical Location & Seq
Forward (+)
53386465 .. 53388929
2465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G029290.1

Sequence Viewer

Length: 1188 bp
ATGGCGCCGGCCATTCCGACGCCGGAGAAAATCGAGCAAGACGAAAATGGCGTCAAGGTCGTAAAGAACATAAATTCCAGAGCCAAATCGGCGACTGACCGACCCAAGAGTATCTTAAAGCAGTCGCCTTTACACCCAAATTTGATGAACAACTCAGAGAATCAAAAAACCCAACAGAAATTCGTGAAATCCAAGTTGAGGGTATCGATCAATTTGCCGGATAATGAAATCTCATTACTGAAAAGCAGACAGTTGAGCTTTGCTGAAGGGAGAGAAGTTAATGATTCTTCATCTTCTTTTTCCCCAAGTAGAGTTTCAAGAGGAAAAGATTACTGTAGAAGCCACAGAACTGTTCTTGAGGTTCTGAGAGAATTGGATGCTGACATTTTGGCTCTGCAAGATGTGAAAGCAGAGGAGGAGAAGGAAATGAAGCCTCTTTCGGATTTGGCCGCCGCTTTGGGGATGAATTATGTCTTCGCCGAGAGTTGGGCGCCGGAGTACGGCAACGCCATCTTGTCAAAATGGCCGATAAAACGGTCTAAGGTTCAGAAAATCTTTGATGACACTGATTTCAGGAACGTTCTAAAAGCCACCATTGACGTACCCCAAGCCGGAGAAGTCAACTTCCACTGCACCCACCTTGATCATCTTGATGAGAACTGGCGGATGAGGCAGATTAACGCTATAATCCAATCGAGCAATGAGCCTCACATCTTGGCTGGAGGTCTCAATTCACTAGAGGAATCGGATTATTCTCAAGAAAGATGGACTGACATTGTAAAGTATTATGAGGAGATGGGAAAACCAACACCTAAGGTCGAAGTAATGAGATACTTGAAGAGCAAGCAGTATACAGATGCCAAGGACTTTGAAGGAGAATATGAGTCGGTAGTTATGATTGCCAAAGGCCAAAGTGTGCAGGGGACATGTAAGTATGGAACACGGGTAGATTACATATTGGCGTCCTCAAATTCGCCCTACAAGTTTGTTCCCGGATCATACTCTGTCTTCTCATCCAAAGGGACATCTGATCATCATATAGTAAGAGTTGATGTGATCAAATCGGATAGTGGTTGTGATCCAGGAAATGTCTCCAGTCGAGGGCAAAGGAAACAGAAAGTTATAAAGATATCGGAGACTACTCCATCAAAAGGCATATGGAAAACAGAGATAAGTTCAGAAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

395

Amino Acids

44.46

Weight (kDa)

8.13

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 111 - 345 5.2e-13 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1124
AccB1I GGYRCC 2 cut(s) 4, 490
AccI GTMKAC 1 cut(s) 851
AciI CCGC 3 cut(s) 450, 453, 664
AclI AACGTT 1 cut(s) 579
AclWI GGATC 2 cut(s) 1003, 1073
AcoI YGGCCR 3 cut(s) 9, 447, 524
AcsI RAATTY 4 cut(s) 73, 139, 179, 970
AcuI CTGAAG 1 cut(s) 285
AcyI GRCGYC 5 cut(s) 5, 20, 51, 491, 962
AfaI GTAC 2 cut(s) 500, 603
AfiI CCNNNNNNNGG 7 cut(s) 198, 459, 486, 500, 611, 1101, 1151
AflIII ACRYGT 1 cut(s) 926
AgsI TTSAA 3 cut(s) 318, 838, 872
AjnI CCWGG 1 cut(s) 1081
AjuI GAANNNNNNNTTGG 2 cut(s) 298, 330
AloI GAACNNNNNNTCC 2 cut(s) 59, 91
AluBI AGCT 1 cut(s) 258
AluI AGCT 1 cut(s) 258
Alw26I GTCTC 3 cut(s) 731, 1096, 1130
AlwI GGATC 2 cut(s) 1003, 1073
AoxI GGCC 4 cut(s) 9, 447, 524, 907
ApoI RAATTY 4 cut(s) 73, 139, 179, 970
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
AspLEI GCGC 2 cut(s) 7, 493
AsuC2I CCSGG 1 cut(s) 993
AxyI CCTNAGG 1 cut(s) 813
BanI GGYRCC 2 cut(s) 4, 490
BbsI GAAGAC 2 cut(s) 466, 1000
BccI CCATC 4 cut(s) 518, 759, 790, 1153
BceAI ACGGC 1 cut(s) 517
BcgI CGANNNNNNTGC 2 cut(s) 196, 230
BciT130I CCWGG 1 cut(s) 1083
BclI TGATCA 3 cut(s) 643, 1030, 1056
BcnI CCSGG 1 cut(s) 993
BcoDI GTCTC 3 cut(s) 731, 1096, 1130
BfaI CTAG 1 cut(s) 737
BfmI CTRYAG 1 cut(s) 334
BfoI RGCGCY 2 cut(s) 8, 494
BglI GCCNNNNNGGC 1 cut(s) 89
BisI GCNGC 2 cut(s) 450, 453
BlsI GCNGC 2 cut(s) 451, 454
Bme1390I CCNGG 2 cut(s) 993, 1083
BmiI GGNNCC 2 cut(s) 6, 492
BmrFI CCNGG 2 cut(s) 993, 1083
BmsI GCATC 2 cut(s) 367, 847
BpiI GAAGAC 2 cut(s) 466, 1000
BpmI CTGGAG 2 cut(s) 741, 1078
BpuEI CTTGAG 2 cut(s) 377, 741
BpuMI CCSGG 1 cut(s) 993
Bsa29I ATCGAT 1 cut(s) 206
BsaHI GRCGYC 5 cut(s) 5, 20, 51, 491, 962
BsaI GGTCTC 1 cut(s) 731
BsaJI CCNNGG 1 cut(s) 861
Bsc4I CCNNNNNNNGG 7 cut(s) 198, 459, 486, 500, 611, 1101, 1151
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 2 cut(s) 665, 1095
Bse21I CCTNAGG 1 cut(s) 813
Bse3DI GCAATG 1 cut(s) 706
BseBI CCWGG 1 cut(s) 1083
BseCI ATCGAT 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 861
BseGI GGATG 4 cut(s) 382, 468, 672, 1013
BseLI CCNNNNNNNGG 7 cut(s) 198, 459, 486, 500, 611, 1101, 1151
BseMI GCAATG 1 cut(s) 706
BseMII CTCAG 2 cut(s) 168, 356
BseNI ACTGG 2 cut(s) 665, 1095
BseRI GAGGAG 3 cut(s) 428, 431, 806
BsgI GTGCAG 2 cut(s) 616, 938
BshFI GGCC 4 cut(s) 11, 449, 526, 909
BshNI GGYRCC 2 cut(s) 4, 490
BshVI ATCGAT 1 cut(s) 206
BsiSI CCGG 6 cut(s) 8, 23, 218, 494, 612, 993
BslFI GGGAC 2 cut(s) 937, 1036
BslI CCNNNNNNNGG 7 cut(s) 198, 459, 486, 500, 611, 1101, 1151
BsmAI GTCTC 3 cut(s) 731, 1096, 1130
BsmFI GGGAC 2 cut(s) 937, 1036
BsnI GGCC 4 cut(s) 11, 449, 526, 909
Bso31I GGTCTC 1 cut(s) 731
Bsp143I GATC 6 cut(s) 207, 643, 995, 1030, 1056, 1078
BspACI CCGC 3 cut(s) 450, 453, 664
BspANI GGCC 4 cut(s) 11, 449, 526, 909
BspCNI CTCAG 2 cut(s) 167, 357
BspDI ATCGAT 1 cut(s) 206
BspLI GGNNCC 2 cut(s) 6, 492
BspPI GGATC 2 cut(s) 1003, 1073
BspQI GCTCTTC 1 cut(s) 833
BspT107I GGYRCC 2 cut(s) 4, 490
BspTNI GGTCTC 1 cut(s) 731
BsrDI GCAATG 1 cut(s) 706
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 2 cut(s) 665, 1095
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 1 cut(s) 861
BssMI GATC 6 cut(s) 207, 643, 995, 1030, 1056, 1078
BssNAI GTATAC 1 cut(s) 852
BssNI GRCGYC 5 cut(s) 5, 20, 51, 491, 962
BssT1I CCWWGG 1 cut(s) 861
Bst1107I GTATAC 1 cut(s) 852
Bst2UI CCWGG 1 cut(s) 1083
Bst4CI ACNGT 4 cut(s) 252, 335, 352, 537
Bst6I CTCTTC 1 cut(s) 833
BstACI GRCGYC 5 cut(s) 5, 20, 51, 491, 962
BstC8I GCNNGC 2 cut(s) 9, 845
BstDEI CTNAG 4 cut(s) 154, 365, 540, 813
BstF5I GGATG 4 cut(s) 382, 468, 672, 1013
BstH2I RGCGCY 2 cut(s) 8, 494
BstHHI GCGC 2 cut(s) 7, 493
BstKTI GATC 6 cut(s) 210, 646, 998, 1033, 1059, 1081
BstMAI GTCTC 3 cut(s) 731, 1096, 1130
BstMBI GATC 6 cut(s) 207, 643, 995, 1030, 1056, 1078
BstMWI GCNNNNNNNGC 2 cut(s) 89, 670
BstNI CCWGG 1 cut(s) 1083
BstNSI RCATGY 1 cut(s) 930
BstSCI CCNGG 2 cut(s) 991, 1081
BstSFI CTRYAG 1 cut(s) 334
BstV2I GAAGAC 2 cut(s) 466, 1000
BstZ17I GTATAC 1 cut(s) 852
Bsu15I ATCGAT 1 cut(s) 206
Bsu36I CCTNAGG 1 cut(s) 813
BsuRI GGCC 4 cut(s) 11, 449, 526, 909
BsuTUI ATCGAT 1 cut(s) 206
BtsCI GGATG 4 cut(s) 382, 468, 672, 1013
BtsI GCAGTG 1 cut(s) 628
BtsIMutI CAGTG 2 cut(s) 564, 628
Cac8I GCNNGC 2 cut(s) 9, 845
CfoI GCGC 2 cut(s) 7, 493
Cfr10I RCCGGY 1 cut(s) 7
ClaI ATCGAT 1 cut(s) 206
CseI GACGC 3 cut(s) 28, 40, 951
Csp6I GTAC 2 cut(s) 499, 602
CviAII CATG 1 cut(s) 927
CviQI GTAC 2 cut(s) 499, 602
DdeI CTNAG 4 cut(s) 154, 365, 540, 813
DinI GGCGCC 2 cut(s) 6, 492
DpnI GATC 6 cut(s) 209, 645, 997, 1032, 1058, 1080
DpnII GATC 6 cut(s) 207, 643, 995, 1030, 1056, 1078
EaeI YGGCCR 3 cut(s) 9, 447, 524
Eam1104I CTCTTC 1 cut(s) 833
EarI CTCTTC 1 cut(s) 833
EciI GGCGGA 1 cut(s) 679
Eco130I CCWWGG 1 cut(s) 861
Eco31I GGTCTC 1 cut(s) 731
Eco32I GATATC 1 cut(s) 1131
Eco57I CTGAAG 1 cut(s) 285
Eco81I CCTNAGG 1 cut(s) 813
EcoRII CCWGG 1 cut(s) 1081
EcoRV GATATC 1 cut(s) 1131
EcoT14I CCWWGG 1 cut(s) 861
EgeI GGCGCC 2 cut(s) 6, 492
EheI GGCGCC 2 cut(s) 6, 492
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 1 cut(s) 930
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FaqI GGGAC 2 cut(s) 937, 1036
FatI CATG 1 cut(s) 926
FauNDI CATATG 1 cut(s) 1157
FbaI TGATCA 3 cut(s) 643, 1030, 1056
FblI GTMKAC 1 cut(s) 851
Fnu4HI GCNGC 2 cut(s) 450, 453
FokI GGATG 4 cut(s) 389, 475, 679, 1000
Fsp4HI GCNGC 2 cut(s) 450, 453
FspBI CTAG 1 cut(s) 737
GlaI GCGC 2 cut(s) 6, 492
GluI GCNGC 2 cut(s) 450, 453
GsuI CTGGAG 2 cut(s) 741, 1078
HaeII RGCGCY 2 cut(s) 8, 494
HaeIII GGCC 4 cut(s) 11, 449, 526, 909
HapII CCGG 6 cut(s) 8, 23, 218, 494, 612, 993
HgaI GACGC 3 cut(s) 28, 40, 951
HhaI GCGC 2 cut(s) 7, 493
Hin1I GRCGYC 5 cut(s) 5, 20, 51, 491, 962
Hin1II CATG 1 cut(s) 930
Hin6I GCGC 2 cut(s) 5, 491
HinP1I GCGC 2 cut(s) 5, 491
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HinfI GANTC 4 cut(s) 160, 284, 743, 884
HpaII CCGG 6 cut(s) 8, 23, 218, 494, 612, 993
Hpy166II GTNNAC 2 cut(s) 622, 852
Hpy188III TCNNGA 7 cut(s) 78, 184, 318, 356, 574, 650, 758
Hpy8I GTNNAC 2 cut(s) 622, 852
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 4 cut(s) 260, 415, 866, 1175
HpyCH4III ACNGT 4 cut(s) 252, 335, 352, 537
HpyCH4IV ACGT 2 cut(s) 579, 600
HpyCH4V TGCA 3 cut(s) 397, 633, 919
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 670
HpyF3I CTNAG 4 cut(s) 154, 365, 540, 813
HpySE526I ACGT 2 cut(s) 579, 600
Hsp92I GRCGYC 5 cut(s) 5, 20, 51, 491, 962
Hsp92II CATG 1 cut(s) 930
HspAI GCGC 2 cut(s) 5, 491
KasI GGCGCC 2 cut(s) 4, 490
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
Ksp22I TGATCA 3 cut(s) 643, 1030, 1056
Kzo9I GATC 6 cut(s) 207, 643, 995, 1030, 1056, 1078
LguI GCTCTTC 1 cut(s) 833
LweI GCATC 2 cut(s) 367, 847
MaeI CTAG 1 cut(s) 737
MaeII ACGT 2 cut(s) 579, 600
MalI GATC 6 cut(s) 209, 645, 997, 1032, 1058, 1080
MboI GATC 6 cut(s) 207, 643, 995, 1030, 1056, 1078
MboII GAAGA 5 cut(s) 279, 285, 466, 850, 1000
MluCI AATT 8 cut(s) 73, 139, 179, 211, 371, 466, 730, 970
Mly113I GGCGCC 2 cut(s) 5, 491
MlyI GAGTC 1 cut(s) 893
MmeI TCCRAC 1 cut(s) 41
MroNI GCCGGC 1 cut(s) 7
MseI TTAA 3 cut(s) 116, 279, 678
MslI CAYNNNNRTG 1 cut(s) 651
MspI CCGG 6 cut(s) 8, 23, 218, 494, 612, 993
MspR9I CCNGG 2 cut(s) 993, 1083
MvaI CCWGG 1 cut(s) 1083
MwoI GCNNNNNNNGC 2 cut(s) 89, 670
NaeI GCCGGC 1 cut(s) 9
NarI GGCGCC 2 cut(s) 5, 491
NciI CCSGG 1 cut(s) 993
NdeI CATATG 1 cut(s) 1157
NdeII GATC 6 cut(s) 207, 643, 995, 1030, 1056, 1078
NgoMIV GCCGGC 1 cut(s) 7
NlaIII CATG 1 cut(s) 930
NlaIV GGNNCC 2 cut(s) 6, 492
NmeAIII GCCGAG 1 cut(s) 505
NspI RCATGY 1 cut(s) 930
PciI ACATGT 1 cut(s) 926
PciSI GCTCTTC 1 cut(s) 833
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 48
PdiI GCCGGC 1 cut(s) 9
PfeI GAWTC 3 cut(s) 160, 284, 743
PfoI TCCNGGA 2 cut(s) 991, 1081
PkrI GCNGC 2 cut(s) 451, 454
PleI GAGTC 1 cut(s) 892
PluTI GGCGCC 2 cut(s) 8, 494
PpsI GAGTC 1 cut(s) 892
PscI ACATGT 1 cut(s) 926
PsiI TTATAA 1 cut(s) 1124
Psp1406I AACGTT 1 cut(s) 579
Psp6I CCWGG 1 cut(s) 1081
PspGI CCWGG 1 cut(s) 1081
PspN4I GGNNCC 2 cut(s) 6, 492
RsaI GTAC 2 cut(s) 500, 603
RsaNI GTAC 2 cut(s) 499, 602
RseI CAYNNNNRTG 1 cut(s) 651
SapI GCTCTTC 1 cut(s) 833
SaqAI TTAA 3 cut(s) 116, 279, 678
SatI GCNGC 2 cut(s) 450, 453
Sau3AI GATC 6 cut(s) 207, 643, 995, 1030, 1056, 1078
SchI GAGTC 1 cut(s) 893
ScrFI CCNGG 2 cut(s) 993, 1083
SfaNI GCATC 2 cut(s) 367, 847
SfcI CTRYAG 1 cut(s) 334
SfoI GGCGCC 2 cut(s) 6, 492
SmiMI CAYNNNNRTG 1 cut(s) 651
SmlI CTYRAG 2 cut(s) 356, 756
SmoI CTYRAG 2 cut(s) 356, 756
Sse9I AATT 8 cut(s) 73, 139, 179, 211, 371, 466, 730, 970
SsiI CCGC 3 cut(s) 450, 453, 664
SspDI GGCGCC 2 cut(s) 4, 490
SspMI CTAG 1 cut(s) 737
StyD4I CCNGG 2 cut(s) 991, 1081
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 4 cut(s) 252, 335, 352, 537
TaiI ACGT 2 cut(s) 582, 603
TaqI TCGA 5 cut(s) 33, 206, 695, 819, 1099
TaqII GACCGA 1 cut(s) 114
TasI AATT 8 cut(s) 73, 139, 179, 211, 371, 466, 730, 970
TauI GCSGC 2 cut(s) 452, 455
TfiI GAWTC 3 cut(s) 160, 284, 743
Tru1I TTAA 3 cut(s) 116, 279, 678
Tru9I TTAA 3 cut(s) 116, 279, 678
TscAI CASTG 2 cut(s) 571, 635
TspDTI ATGAA 5 cut(s) 161, 240, 279, 443, 479
TspRI CASTG 2 cut(s) 571, 635
XapI RAATTY 4 cut(s) 73, 139, 179, 970
XceI RCATGY 1 cut(s) 930
XmiI GTMKAC 1 cut(s) 851
XspI CTAG 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.