Rorug06G0223100

Endonuclease/Exonuclease/phosphatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010396
Physical Location & Seq
Forward (+)
35680344 .. 35682225
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0223100.1

Sequence Viewer

Length: 1233 bp
ATGGAGAAAGTAGAGGTAGTCATAGTAGGTGCAGGTCCTGCTGGTTTAGCAACCTCTGCATGTCTTAATCGCCTCAATATCTCAAATGTCGTACTCGAAAGAGAAGATTGCTATGCTTCTCTTTGGAAGAATAAGTCTTATGATCGTTTGAAGCTTCACTTAGCAAAGCAATTCTGTGAACTACCCTATATGCCATTCCCTAAAAATGCACCCACATATGTTCCCAGGAAAGAGTTTATTCAATACCTAGATACTTATATGTCCACTTTCAAGATAAACCCTCTGTACCACAGAAGTGTGGAGACTGCTTTCTACGATGAGGATGTGGAAAAATGGTATGTCATTGTCAACAACACAGAACTAGGTGTACAAGAAAGCTACTGCGGGAAGTTTCTTGTGGTAGCGACCGGTGAAAATAGTGAAGGATATCTTCCTCAAACGAAAGGTTTGAGTAGCTTTAAAGGCGAAATCATTCATTCGAGTGAGTATGGAAATGGCAAGAAGTACCGCGGCAAAAATGTTTTAGTTGTCGGATCTGGGAATTCTGGAATGGAAATTGCCTACGACTTATCAAATTTCGGGGCCAATACTTCTATTGTTGTCCGTAGCCCGGTACATGTTCTTACCAAGGAGATCGTATTCATTGGAATGGTTCTGTTAAAGTACCTTCCCGTTATGGTTGTAGACGGCATTGTCATGTTCTTAACCAAGTGGAAATTTGGAAATTTATCCAAGTATGGGATTCAAATTCCAACAATGGGGCCATTTTATGCCAAGGAAAACAAAGGTCAATCCCCCATCATCGATGTTGGGACTATTGAAAAGATCAAGAGCGGAGAAATACAGGTTTTGCCGTCCATAACAAGTATAGAAGGAAATGAAATTTGGTTTGAGAGTGGCTACCTTAAATGTTATGATGCTATAGTATTTGCTACAGGCTATAAAAGCACTGTTCTAAAGTGGCTCAAGGATGAAAACAACCTCTTTAGTGACAATGGAATGCCGAAGAAAAATTTCCCCAACCACTGGAAGTCACAAAATGACCTTTACTGTGTCGGATTCTCTAGGCGTGGATTATTTGGCATTTCACATGATGCACAAAATATAGCAAATGATATAAGCTTCTCTCTCAGTCAGATCCAAAAAAAATGTGATAAATCATTCCAAAGGGTGTCGAGGCTTGAAAGAAGTAGTAGACTACACATGAATTTCCATAGACATTGTCACATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

410

Amino Acids

46.46

Weight (kDa)

8.86

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 5 - 324 3.5e-24 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 5 - 204 1.7e-14 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 7 - 204 6.7e-21 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 68 - 199 4.6e-14 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 692
Acc36I ACCTGC 1 cut(s) 23
AccB7I CCANNNNNTGG 1 cut(s) 1026
AccBSI CCGCTC 1 cut(s) 834
AccI GTMKAC 2 cut(s) 684, 1195
AccII CGCG 1 cut(s) 510
AciI CCGC 4 cut(s) 384, 508, 510, 834
AclWI GGATC 2 cut(s) 541, 1132
AcsI RAATTY 8 cut(s) 541, 574, 716, 724, 747, 882, 1012, 1207
AfaI GTAC 6 cut(s) 93, 287, 369, 506, 615, 665
AfiI CCNNNNNNNGG 4 cut(s) 610, 738, 758, 1026
AflIII ACRYGT 1 cut(s) 616
AgeI ACCGGT 1 cut(s) 407
AgsI TTSAA 6 cut(s) 151, 242, 271, 746, 821, 1184
AjnI CCWGG 1 cut(s) 224
AjuI GAANNNNNNNTTGG 2 cut(s) 868, 900
AleI CACNNNNGTG 1 cut(s) 294
AluBI AGCT 4 cut(s) 154, 378, 456, 1122
AluI AGCT 4 cut(s) 154, 378, 456, 1122
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 2 cut(s) 541, 1132
AlwNI CAGNNNCTG 1 cut(s) 38
AoxI GGCC 2 cut(s) 582, 761
ApoI RAATTY 8 cut(s) 541, 574, 716, 724, 747, 882, 1012, 1207
AsiGI ACCGGT 1 cut(s) 407
Asp700I GAANNNNTTC 2 cut(s) 471, 1013
AspS9I GGNCC 3 cut(s) 35, 582, 761
AsuC2I CCSGG 1 cut(s) 611
AsuHPI GGTGA 1 cut(s) 422
AvaII GGWCC 1 cut(s) 35
BccI CCATC 1 cut(s) 806
BceAI ACGGC 2 cut(s) 703, 838
BciT130I CCWGG 1 cut(s) 226
BcnI CCSGG 1 cut(s) 611
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 3 cut(s) 248, 362, 1065
BfmI CTRYAG 2 cut(s) 921, 933
BfuAI ACCTGC 1 cut(s) 23
BisI GCNGC 1 cut(s) 511
BlsI GCNGC 1 cut(s) 512
Bme1390I CCNGG 2 cut(s) 226, 611
Bme18I GGWCC 1 cut(s) 35
BmgT120I GGNCC 3 cut(s) 35, 582, 761
BmiI GGNNCC 2 cut(s) 583, 762
BmrFI CCNGG 2 cut(s) 226, 611
BmsI GCATC 2 cut(s) 907, 1084
BpuEI CTTGAG 1 cut(s) 950
BpuMI CCSGG 1 cut(s) 611
Bsa29I ATCGAT 1 cut(s) 804
BsaJI CCNNGG 4 cut(s) 224, 508, 627, 774
BsaWI WCCGGW 1 cut(s) 407
Bsc4I CCNNNNNNNGG 4 cut(s) 610, 738, 758, 1026
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 1 cut(s) 1031
BseBI CCWGG 1 cut(s) 226
BseCI ATCGAT 1 cut(s) 804
BseDI CCNNGG 4 cut(s) 224, 508, 627, 774
BseGI GGATG 2 cut(s) 328, 976
BseLI CCNNNNNNNGG 4 cut(s) 610, 738, 758, 1026
BseMII CTCAG 1 cut(s) 1144
BseNI ACTGG 1 cut(s) 1031
BsgI GTGCAG 1 cut(s) 51
Bsh1236I CGCG 1 cut(s) 510
Bsh1285I CGRYCG 1 cut(s) 408
BshFI GGCC 2 cut(s) 584, 763
BshTI ACCGGT 1 cut(s) 407
BshVI ATCGAT 1 cut(s) 804
BsiEI CGRYCG 1 cut(s) 408
BsiSI CCGG 2 cut(s) 408, 611
BslFI GGGAC 1 cut(s) 826
BslI CCNNNNNNNGG 4 cut(s) 610, 738, 758, 1026
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 826
BsmI GAATGC 1 cut(s) 1005
BsnI GGCC 2 cut(s) 584, 763
Bsp1407I TGTACA 1 cut(s) 367
Bsp143I GATC 5 cut(s) 142, 533, 633, 825, 1137
BspACI CCGC 4 cut(s) 384, 508, 510, 834
BspANI GGCC 2 cut(s) 584, 763
BspCNI CTCAG 1 cut(s) 1143
BspDI ATCGAT 1 cut(s) 804
BspFNI CGCG 1 cut(s) 510
BspLI GGNNCC 2 cut(s) 583, 762
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 2 cut(s) 541, 1132
BsrBI CCGCTC 1 cut(s) 834
BsrFI RCCGGY 1 cut(s) 407
BsrGI TGTACA 1 cut(s) 367
BsrI ACTGG 1 cut(s) 1031
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 4 cut(s) 224, 508, 627, 774
BssMI GATC 5 cut(s) 142, 533, 633, 825, 1137
BssT1I CCWWGG 2 cut(s) 627, 774
Bst2UI CCWGG 1 cut(s) 226
Bst4CI ACNGT 2 cut(s) 952, 1052
BstAPI GCANNNNNTGC 2 cut(s) 38, 56
BstAUI TGTACA 1 cut(s) 367
BstDEI CTNAG 2 cut(s) 160, 1130
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 2 cut(s) 328, 976
BstFNI CGCG 1 cut(s) 510
BstKTI GATC 5 cut(s) 145, 536, 636, 828, 1140
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 5 cut(s) 142, 533, 633, 825, 1137
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 462, 945
BstNI CCWGG 1 cut(s) 226
BstNSI RCATGY 2 cut(s) 63, 620
BstSCI CCNGG 2 cut(s) 224, 609
BstSFI CTRYAG 2 cut(s) 921, 933
BstUI CGCG 1 cut(s) 510
BstX2I RGATCY 2 cut(s) 533, 1137
BstYI RGATCY 2 cut(s) 533, 1137
Bsu15I ATCGAT 1 cut(s) 804
BsuRI GGCC 2 cut(s) 584, 763
BsuTUI ATCGAT 1 cut(s) 804
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 2 cut(s) 328, 976
BtsIMutI CAGTG 2 cut(s) 948, 1024
BveI ACCTGC 1 cut(s) 23
CaiI CAGNNNCTG 1 cut(s) 38
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 3 cut(s) 35, 582, 761
Cfr42I CCGCGG 1 cut(s) 511
ClaI ATCGAT 1 cut(s) 804
Csp6I GTAC 6 cut(s) 92, 286, 368, 505, 614, 664
CspAI ACCGGT 1 cut(s) 407
CviAII CATG 5 cut(s) 60, 617, 697, 1091, 1204
CviQI GTAC 6 cut(s) 92, 286, 368, 505, 614, 664
DdeI CTNAG 2 cut(s) 160, 1130
DpnI GATC 5 cut(s) 144, 535, 635, 827, 1139
DpnII GATC 5 cut(s) 142, 533, 633, 825, 1137
DraI TTTAAA 1 cut(s) 460
DrdI GACNNNNNNGTC 1 cut(s) 692
DseDI GACNNNNNNGTC 1 cut(s) 692
Eco130I CCWWGG 2 cut(s) 627, 774
Eco32I GATATC 1 cut(s) 428
Eco47I GGWCC 1 cut(s) 35
EcoO109I RGGNCCY 1 cut(s) 35
EcoRI GAATTC 1 cut(s) 541
EcoRII CCWGG 1 cut(s) 224
EcoRV GATATC 1 cut(s) 428
EcoT14I CCWWGG 2 cut(s) 627, 774
ErhI CCWWGG 2 cut(s) 627, 774
FaeI CATG 5 cut(s) 63, 620, 700, 1094, 1207
FalI AAGNNNNNCTT 4 cut(s) 143, 175, 414, 446
FaqI GGGAC 1 cut(s) 826
FatI CATG 5 cut(s) 59, 616, 696, 1090, 1203
FauI CCCGC 1 cut(s) 377
FauNDI CATATG 1 cut(s) 217
FblI GTMKAC 2 cut(s) 684, 1195
Fnu4HI GCNGC 1 cut(s) 511
FokI GGATG 2 cut(s) 335, 983
Fsp4HI GCNGC 1 cut(s) 511
FspBI CTAG 3 cut(s) 248, 362, 1065
GluI GCNGC 1 cut(s) 511
HaeIII GGCC 2 cut(s) 584, 763
HapII CCGG 2 cut(s) 408, 611
Hin1II CATG 5 cut(s) 63, 620, 700, 1094, 1207
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 2 cut(s) 152, 1120
HinfI GANTC 2 cut(s) 742, 1059
HpaII CCGG 2 cut(s) 408, 611
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 6 cut(s) 179, 264, 349, 368, 685, 1196
Hpy188I TCNGA 3 cut(s) 533, 1058, 1137
Hpy188III TCNNGA 3 cut(s) 271, 546, 829
Hpy8I GTNNAC 6 cut(s) 179, 264, 349, 368, 685, 1196
HpyAV CCTTC 3 cut(s) 416, 677, 866
HpyCH4III ACNGT 2 cut(s) 952, 1052
HpyCH4V TGCA 4 cut(s) 32, 59, 209, 1097
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 462, 945
HpyF3I CTNAG 2 cut(s) 160, 1130
Hsp92II CATG 5 cut(s) 63, 620, 700, 1094, 1207
KspI CCGCGG 1 cut(s) 511
Kzo9I GATC 5 cut(s) 142, 533, 633, 825, 1137
LweI GCATC 2 cut(s) 907, 1084
MaeI CTAG 3 cut(s) 248, 362, 1065
MaeIII GTNAC 3 cut(s) 989, 1032, 1223
MalI GATC 5 cut(s) 144, 535, 635, 827, 1139
MbiI CCGCTC 1 cut(s) 834
MboI GATC 5 cut(s) 142, 533, 633, 825, 1137
MboII GAAGA 4 cut(s) 116, 139, 422, 1018
MflI RGATCY 2 cut(s) 533, 1137
MmeI TCCRAC 3 cut(s) 511, 776, 1036
MnlI CCTC 8 cut(s) 7, 64, 83, 291, 313, 444, 992, 1170
MroXI GAANNNNTTC 2 cut(s) 471, 1013
MseI TTAA 5 cut(s) 66, 459, 659, 704, 906
MslI CAYNNNNRTG 4 cut(s) 294, 480, 647, 695
MspA1I CMGCKG 1 cut(s) 510
MspI CCGG 2 cut(s) 408, 611
MspR9I CCNGG 2 cut(s) 226, 611
Mva1269I GAATGC 1 cut(s) 1005
MvaI CCWGG 1 cut(s) 226
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 462, 945
NciI CCSGG 1 cut(s) 611
NdeI CATATG 1 cut(s) 217
NdeII GATC 5 cut(s) 142, 533, 633, 825, 1137
NlaIII CATG 5 cut(s) 63, 620, 700, 1094, 1207
NlaIV GGNNCC 2 cut(s) 583, 762
NmuCI GTSAC 3 cut(s) 989, 1032, 1223
NspI RCATGY 2 cut(s) 63, 620
OliI CACNNNNGTG 1 cut(s) 294
PciI ACATGT 1 cut(s) 616
PctI GAATGC 1 cut(s) 1005
PdmI GAANNNNTTC 2 cut(s) 471, 1013
PfeI GAWTC 2 cut(s) 742, 1059
PflFI GACNNNGTC 1 cut(s) 1221
PflMI CCANNNNNTGG 1 cut(s) 1026
PinAI ACCGGT 1 cut(s) 407
PkrI GCNGC 1 cut(s) 512
PpuMI RGGWCCY 1 cut(s) 35
PscI ACATGT 1 cut(s) 616
Psp5II RGGWCCY 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 224
PspGI CCWGG 1 cut(s) 224
PspN4I GGNNCC 2 cut(s) 583, 762
PspPI GGNCC 3 cut(s) 35, 582, 761
PspPPI RGGWCCY 1 cut(s) 35
PsrI GAACNNNNNNTAC 2 cut(s) 351, 383
PstNI CAGNNNCTG 1 cut(s) 38
PsuI RGATCY 2 cut(s) 533, 1137
PsyI GACNNNGTC 1 cut(s) 1221
RsaI GTAC 6 cut(s) 93, 287, 369, 506, 615, 665
RsaNI GTAC 6 cut(s) 92, 286, 368, 505, 614, 664
RseI CAYNNNNRTG 4 cut(s) 294, 480, 647, 695
SacII CCGCGG 1 cut(s) 511
SaqAI TTAA 5 cut(s) 66, 459, 659, 704, 906
SatI GCNGC 1 cut(s) 511
Sau3AI GATC 5 cut(s) 142, 533, 633, 825, 1137
Sau96I GGNCC 3 cut(s) 35, 582, 761
ScrFI CCNGG 2 cut(s) 226, 611
SfaNI GCATC 2 cut(s) 907, 1084
SfcI CTRYAG 2 cut(s) 921, 933
Sfr303I CCGCGG 1 cut(s) 511
SgrBI CCGCGG 1 cut(s) 511
SinI GGWCC 1 cut(s) 35
SmiMI CAYNNNNRTG 4 cut(s) 294, 480, 647, 695
SmlI CTYRAG 1 cut(s) 965
SmoI CTYRAG 1 cut(s) 965
SsiI CCGC 4 cut(s) 384, 508, 510, 834
SspMI CTAG 3 cut(s) 248, 362, 1065
StyD4I CCNGG 2 cut(s) 224, 609
StyI CCWWGG 2 cut(s) 627, 774
TaaI ACNGT 2 cut(s) 952, 1052
TaqI TCGA 4 cut(s) 96, 479, 804, 1175
TatI WGTACW 1 cut(s) 367
TauI GCSGC 1 cut(s) 513
TfiI GAWTC 2 cut(s) 742, 1059
Tru1I TTAA 5 cut(s) 66, 459, 659, 704, 906
Tru9I TTAA 5 cut(s) 66, 459, 659, 704, 906
TscAI CASTG 2 cut(s) 955, 1031
TseFI GTSAC 3 cut(s) 989, 1032, 1223
Tsp45I GTSAC 3 cut(s) 989, 1032, 1223
TspDTI ATGAA 5 cut(s) 464, 631, 894, 987, 1220
TspGWI ACGGA 1 cut(s) 593
TspRI CASTG 2 cut(s) 955, 1031
Tth111I GACNNNGTC 1 cut(s) 1221
Van91I CCANNNNNTGG 1 cut(s) 1026
VpaK11BI GGWCC 1 cut(s) 35
XapI RAATTY 8 cut(s) 541, 574, 716, 724, 747, 882, 1012, 1207
XceI RCATGY 2 cut(s) 63, 620
XmiI GTMKAC 2 cut(s) 684, 1195
XmnI GAANNNNTTC 2 cut(s) 471, 1013
XspI CTAG 3 cut(s) 248, 362, 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.