Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0007962

Physical Location & Seq

Genomic Coordinates Reverse (-)

20829082 .. 20834643

Gene Structure 5562 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g25810.t6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2205 bp

ATGCTACACGAGTTGTTACTGGCACTAGTGGGCTACACCGGCGATCTCATAATCGACGAGAGAGAGCACTCCTCTTCTCTCGGAATCGACTCCCCCATTTCCGACCACCCTACTTTCCACCTCGCTCCGGACATCTCCTTCATCCAACCCAGCGAAAGGGATCTCATACAAAGAATCGTCACCTTAGGGTTTTACTACCGAGAGCTTGACCGCTTCGCCACCAGGTCTCGGAATTTGAGCTGGATAAGGTCTGCTAAGCCGAGTCCGGTACTCGCCGGAAAAGTTGAGAAGCCGAGCGTGTACCGGAGAGCCGTTGCCAACGGAATTGTGGAGATTCTCTCGGTTTATAGGTCGGCGGTTCTTCACATTGAGCAGAAATTGTTGGCTGAGTCGGTTCCTATACTGGCAACTGTGACTCAGGGCCTCACTAAGTTTTTTGTGCTTTTGCCGCCGCTGTATGAGCTCATACTGGAGATTGAGAGGGATGACATTTGCGGCGGTCAGCTGCTGAATCTTTTGCATAAACGATGCCATTGTGGAGTGCCGGAGTTGCAGACGTGCATTCAGAGGCTTCTTTGGCATGCACATCAGGTCATGTACAACCAGTTAGCAGCATGGATGGTGTACGGGATCCTTCAAGACCAGCACGGAGAGTTTTTCATTAGGAGGATGGAAGATAAGGATGTAGAGTCATCTCATCAAGACATATCTGAAAAGCTGGCACGTATGTCAACGGACGACACATCCGATTGGCACTTGGGATTTCATATCTATTTGGATATGCTGCCCGAATATATCCAGATACGTGTTGCAGAATCAGTATTGTTTGCTGGCAAGGCCATCAGGGTTCTTCGCAATCCAAGCCATGCCTTTCGATTTCAGGATGATGTATTACACCAGCAGGTGCCAAGGGGATCTCAGAAAATCCAAGGATTTTCAGGACGTTTCCCATTTCACAAAGACCCTTTTGTTGAGAAAGAATTGATTGGCGAAGAAATGCTTCCACAGTCTGAGGCCGATAAGATTGAAACTATGCTTCTGGAACTAAAGGAATCATCTGAGTTCCACAAAAGATCCTTTGAATGTGCAGTTGACTCAATACGGGCTATTGCTGCCAGTCATCTTTGGCAGCTTGTGGTTGTACGTGCTGACTTGAATGGTCATCTAAAGGCACTGAAAGACTATTTTCTGTTGGCAAAAGGAGATTTCTTTCAGTGTTTTCTTGAAGAGAGCCGCCAATTGATGCGGCTACCTCCTCGCCAGTCAACTGCTGAGGCTGATCTTATGGTCCCTTTCCAGCTGGCTGCTCTTAAGACTATAGGTGAGGAAGACAAACATTTCTCTAGAGTATCCTTGAGGATGCCTTCATTTGGGATGGCTATTAAATCTTCCCAAATAGATGTACCAAAGACAGATGGAAATTCAGGCACTGCACAATCAAGTGCTTCTTCAGATATAACCCTTGATGGTTGGGATGGGATTGCTCTTGAATACTTTGTTGATTGGCCCTTGCAGTTGTTCTTTACCCCAGAAGTGCTCTCTAAGTACTGCCGGGTTTTCCAATATTTGCTGAGGCTCAAGCGAACACAAATGGAATTGGAGAAATCATGGGCCTCTGTAATGCATCAGGATCACACAGATTTTGCCACACATCGGAATGATCGTGTAAAATGCTCGGTATCACAACAGCGAAGGCAGCGAAGCAGACCAATGTGGCGAATTAGAGAGCATATGGCTTTCTTGATCAGAAATCTTCAATTTTATATACAGGTGGATGTGATAGAATCCCAATGGAATGTTCTGCAAGCTCATATCCAAGATTCTCATGACTTTAATGGACTTGTAGGCGTCCATCTGGAGTACTTAAATGCTCTAATTTCACAATCTTTCTTGGACATTGGCTCTTTATCAAGGATACTTGACAGCATAATGAAACTTTGCATTCGTTTCTGCTGGAACATTGAGAACCAAGAAAGCAGTGCAAATACATCTGAATTGGAACATATTATAGAGGAATTCAACAAGAAATCGAACTCCCTGTACACGATATTGCGCAGTAGTAGGCTTGTAGGGAGTCAGCGAGCCCCATTTCTTAGGCGTTTTCTGATGCGTCTGAACTTCAATTCCTTCTTTGAGGCCACAGCTAGAGGTGTGCTGAATGTTGGTAGACCCCACCCTGCAGTGTCTGTTTTGAATCAACAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005911 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009624 GO:0009987 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030054 GO:0030865 GO:0031109 GO:0031122 GO:0032153 GO:0032155 GO:0032991 GO:0034622 GO:0040007 GO:0043015 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043622 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044464 GO:0046785 GO:0048285 GO:0050896 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051704 GO:0051707 GO:0055044 GO:0065003 GO:0070925 GO:0071840 GO:0090307 GO:0097435 GO:0140014 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Spc97_Spc98

Protein Analysis

735

Amino Acids

84.57

Weight (kDa)

6.5

Isoelectric Point (pI)

55.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GCP_N_terminal	PF17681	2 - 182	2.8e-18	Gamma tubulin complex component N-terminal
GCP_C_terminal	PF04130	187 - 513	8e-57	Gamma tubulin complex component C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007962)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53760 AT3G53760 AT3G53760
fragaria_vesca	FvH4_2g25810 FvH4_2g25810 FvH4_2g25810 FvH4_2g25810 FvH4_2g25810
malus_domestica	MD03G1288900.v1.1
prunus_persica	Prupe.8G262200_v2.0.a1 Prupe.8G262200_v2.0.a1
pyrus_communis	pycom03g22900 pycom11g27030
rosa_chinensis	RchiOBHm_Chr6g0294121
rosa_laevigata	RLG00000011897
rosa_multiflora	Rmu_sc0005037.1_g000009
rosa_roxburghii	Rroxscaffold_7G00173630
rosa_rugosa	Rorug06G0241400 Rorug06G0241500 Rorug06G0241600 Rorug06G0241700
rosa_samantha	Rh6AG354000 Rh6DG354800
rosa_wichuraiana	Rw6G030850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	892
Acc16I	TGCGCA	1 cut(s)	2054
Acc36I	ACCTGC	1 cut(s)	892
AccB1I	GGYRCC	1 cut(s)	904
AccI	GTMKAC	1 cut(s)	2167
AccIII	TCCGGA	1 cut(s)	127
AciI	CCGC	8 cut(s)	211, 356, 449, 452, 495, 498, 1234, 1246
AclWI	GGATC	6 cut(s)	168, 625, 638, 922, 1068, 1638
AcsI	RAATTY	3 cut(s)	232, 1420, 2015
AcuI	CTGAAG	1 cut(s)	1434
AcyI	GRCGYC	1 cut(s)	1848
AfaI	GTAC	9 cut(s)	270, 302, 599, 626, 1143, 1404, 1547, 1862, 2042
AfiI	CCNNNNNNNGG	5 cut(s)	127, 156, 228, 1370, 1653
AflII	CTTAAG	1 cut(s)	1310
AflIII	ACRYGT	1 cut(s)	805
AhlI	ACTAGT	1 cut(s)	25
AjiI	CACGTC	1 cut(s)	558
AjnI	CCWGG	1 cut(s)	221
AjuI	GAANNNNNNNTTGG	2 cut(s)	1782, 1814
AloI	GAACNNNNNNTCC	2 cut(s)	2108, 2140
AluBI	AGCT	9 cut(s)	205, 240, 463, 505, 718, 1132, 1300, 1808, 2144
AluI	AGCT	9 cut(s)	205, 240, 463, 505, 718, 1132, 1300, 1808, 2144
Alw21I	GWGCWC	3 cut(s)	69, 465, 1539
Alw26I	GTCTC	1 cut(s)	231
AlwI	GGATC	6 cut(s)	168, 625, 638, 922, 1068, 1638
AlwNI	CAGNNNCTG	3 cut(s)	508, 1430, 2186
Aor13HI	TCCGGA	1 cut(s)	127
AoxI	GGCC	6 cut(s)	421, 837, 1014, 1505, 1611, 2136
ApeKI	GCWGC	7 cut(s)	505, 611, 784, 1112, 1129, 1304, 1696
ApoI	RAATTY	3 cut(s)	232, 1420, 2015
Asp700I	GAANNNNTTC	1 cut(s)	999
AspLEI	GCGC	1 cut(s)	2055
AspS9I	GGNCC	4 cut(s)	421, 1288, 1506, 1611
AsuC2I	CCSGG	1 cut(s)	1553
AsuHPI	GGTGA	2 cut(s)	172, 1334
AvaII	GGWCC	1 cut(s)	1288
AxyI	CCTNAGG	1 cut(s)	184
BamHI	GGATCC	1 cut(s)	630
BanI	GGYRCC	1 cut(s)	904
BanII	GRGCYC	2 cut(s)	465, 2086
BauI	CACGAG	1 cut(s)	8
BbsI	GAAGAC	1 cut(s)	1335
Bbv12I	GWGCWC	3 cut(s)	69, 465, 1539
BbvCI	CCTCAGC	2 cut(s)	1272, 1571
BbvI	GCAGC	7 cut(s)	492, 623, 771, 1099, 1141, 1291, 1708
BccI	CCATC	8 cut(s)	613, 664, 848, 1369, 1409, 1460, 1469, 1860
BceAI	ACGGC	1 cut(s)	296
BciT130I	CCWGG	1 cut(s)	223
BciVI	GTATCC	2 cut(s)	1360, 1908
BclI	TGATCA	1 cut(s)	1743
BcnI	CCSGG	1 cut(s)	1553
BcoDI	GTCTC	1 cut(s)	231
BcuI	ACTAGT	1 cut(s)	25
BfaI	CTAG	3 cut(s)	26, 1344, 2145
BfmI	CTRYAG	2 cut(s)	1317, 2178
BfrI	CTTAAG	1 cut(s)	1310
BfuAI	ACCTGC	1 cut(s)	892
BfuI	GTATCC	2 cut(s)	1360, 1908
BlpI	GCTNAGC	1 cut(s)	255
BmcAI	AGTACT	2 cut(s)	1547, 1862
Bme1390I	CCNGG	2 cut(s)	223, 1553
Bme18I	GGWCC	1 cut(s)	1288
BmgBI	CACGTC	1 cut(s)	558
BmgT120I	GGNCC	4 cut(s)	421, 1288, 1506, 1611
BmiI	GGNNCC	4 cut(s)	396, 632, 906, 1290
BmrFI	CCNGG	2 cut(s)	223, 1553
BmsI	GCATC	5 cut(s)	518, 1233, 1350, 1633, 2097
BpiI	GAAGAC	1 cut(s)	1335
BplI	GAGNNNNNCTC	4 cut(s)	56, 88, 323, 355
BpmI	CTGGAG	2 cut(s)	491, 1877
Bpu10I	CCTNAGC	2 cut(s)	1272, 1571
Bpu1102I	GCTNAGC	1 cut(s)	255
BpuEI	CTTGAG	2 cut(s)	1375, 1562
BpuMI	CCSGG	1 cut(s)	1553
BsaAI	YACGTR	3 cut(s)	725, 806, 1145
BsaHI	GRCGYC	1 cut(s)	1848
BsaI	GGTCTC	1 cut(s)	231
BsaJI	CCNNGG	2 cut(s)	908, 928
BsaWI	WCCGGW	3 cut(s)	127, 265, 303
Bsc4I	CCNNNNNNNGG	5 cut(s)	127, 156, 228, 1370, 1653
Bse118I	RCCGGY	1 cut(s)	38
Bse1I	ACTGG	6 cut(s)	24, 408, 474, 604, 1116, 1261
Bse21I	CCTNAGG	1 cut(s)	184
BseAI	TCCGGA	1 cut(s)	127
BseBI	CCWGG	1 cut(s)	223
BseDI	CCNNGG	2 cut(s)	908, 928
BseLI	CCNNNNNNNGG	5 cut(s)	127, 156, 228, 1370, 1653
BseMII	CTCAG	7 cut(s)	378, 431, 932, 1002, 1050, 1263, 1562
BseNI	ACTGG	6 cut(s)	24, 408, 474, 604, 1116, 1261
BseRI	GAGGAG	2 cut(s)	61, 1245
BseXI	GCAGC	7 cut(s)	492, 623, 771, 1099, 1141, 1291, 1708
BseYI	CCCAGC	1 cut(s)	149
BsgI	GTGCAG	2 cut(s)	1107, 1416
BshFI	GGCC	6 cut(s)	423, 839, 1016, 1507, 1613, 2138
BshNI	GGYRCC	1 cut(s)	904
BsiHKAI	GWGCWC	3 cut(s)	69, 465, 1539
BsiSI	CCGG	7 cut(s)	39, 128, 266, 276, 304, 545, 1552
BslFI	GGGAC	1 cut(s)	1274
BslI	CCNNNNNNNGG	5 cut(s)	127, 156, 228, 1370, 1653
BsmAI	GTCTC	1 cut(s)	231
BsmFI	GGGAC	1 cut(s)	1274
BsmI	GAATGC	2 cut(s)	561, 1941
BsnI	GGCC	6 cut(s)	423, 839, 1016, 1507, 1613, 2138
Bso31I	GGTCTC	1 cut(s)	231
Bsp1286I	GDGCHC	4 cut(s)	69, 465, 1539, 2086
Bsp13I	TCCGGA	1 cut(s)	127
Bsp1407I	TGTACA	2 cut(s)	597, 2040
Bsp143I	GATC	9 cut(s)	43, 160, 630, 914, 1073, 1279, 1630, 1660, 1743
Bsp1720I	GCTNAGC	1 cut(s)	255
BspACI	CCGC	8 cut(s)	211, 356, 449, 452, 495, 498, 1234, 1246
BspANI	GGCC	6 cut(s)	423, 839, 1016, 1507, 1613, 2138
BspCNI	CTCAG	7 cut(s)	379, 430, 931, 1003, 1051, 1264, 1563
BspEI	TCCGGA	1 cut(s)	127
BspHI	TCATGA	1 cut(s)	1825
BspLI	GGNNCC	4 cut(s)	396, 632, 906, 1290
BspMAI	CTGCAG	1 cut(s)	2182
BspMI	ACCTGC	1 cut(s)	892
BspPI	GGATC	6 cut(s)	168, 625, 638, 922, 1068, 1638
BspT107I	GGYRCC	1 cut(s)	904
BspTI	CTTAAG	1 cut(s)	1310
BspTNI	GGTCTC	1 cut(s)	231
BsrFI	RCCGGY	1 cut(s)	38
BsrGI	TGTACA	2 cut(s)	597, 2040
BsrI	ACTGG	6 cut(s)	24, 408, 474, 604, 1116, 1261
BssAI	RCCGGY	1 cut(s)	38
BssECI	CCNNGG	2 cut(s)	908, 928
BssMI	GATC	9 cut(s)	43, 160, 630, 914, 1073, 1279, 1630, 1660, 1743
BssNI	GRCGYC	1 cut(s)	1848
BssSI	CACGAG	1 cut(s)	8
BssT1I	CCWWGG	2 cut(s)	908, 928
Bst2BI	CACGAG	1 cut(s)	8
Bst2UI	CCWGG	1 cut(s)	223
Bst4CI	ACNGT	3 cut(s)	412, 1008, 2202
Bst6I	CTCTTC	2 cut(s)	79, 1221
BstACI	GRCGYC	1 cut(s)	1848
BstAFI	CTTAAG	1 cut(s)	1310
BstAUI	TGTACA	2 cut(s)	597, 2040
BstBAI	YACGTR	3 cut(s)	725, 806, 1145
BstC8I	GCNNGC	6 cut(s)	582, 720, 832, 1302, 1806, 2082
BstHHI	GCGC	1 cut(s)	2055
BstKTI	GATC	9 cut(s)	46, 163, 633, 917, 1076, 1282, 1633, 1663, 1746
BstMAI	GTCTC	1 cut(s)	231
BstMBI	GATC	9 cut(s)	43, 160, 630, 914, 1073, 1279, 1630, 1660, 1743
BstMWI	GCNNNNNNNGC	9 cut(s)	39, 448, 460, 550, 577, 836, 861, 1112, 1696
BstNI	CCWGG	1 cut(s)	223
BstNSI	RCATGY	1 cut(s)	584
BstSCI	CCNGG	2 cut(s)	221, 1551
BstSFI	CTRYAG	2 cut(s)	1317, 2178
BstV1I	GCAGC	7 cut(s)	492, 623, 771, 1099, 1141, 1291, 1708
BstV2I	GAAGAC	1 cut(s)	1335
BstX2I	RGATCY	4 cut(s)	160, 630, 914, 1073
BstYI	RGATCY	4 cut(s)	160, 630, 914, 1073
Bsu36I	CCTNAGG	1 cut(s)	184
BsuI	GTATCC	2 cut(s)	1360, 1908
BsuRI	GGCC	6 cut(s)	423, 839, 1016, 1507, 1613, 2138
BtrI	CACGTC	1 cut(s)	558
BtsI	GCAGTG	3 cut(s)	1428, 1984, 2187
BtsIMutI	CAGTG	5 cut(s)	1172, 1220, 1428, 1984, 2187
BveI	ACCTGC	1 cut(s)	892
Cac8I	GCNNGC	6 cut(s)	582, 720, 832, 1302, 1806, 2082
CaiI	CAGNNNCTG	3 cut(s)	508, 1430, 2186
CciI	TCATGA	1 cut(s)	1825
CfoI	GCGC	1 cut(s)	2055
Cfr10I	RCCGGY	1 cut(s)	38
Cfr13I	GGNCC	4 cut(s)	421, 1288, 1506, 1611
CseI	GACGC	2 cut(s)	1837, 2099
CsiI	ACCWGGT	1 cut(s)	221
Csp6I	GTAC	9 cut(s)	269, 301, 598, 625, 1142, 1403, 1546, 1861, 2041
CviAII	CATG	6 cut(s)	581, 595, 615, 866, 1608, 1826
CviQI	GTAC	9 cut(s)	269, 301, 598, 625, 1142, 1403, 1546, 1861, 2041
DpnI	GATC	9 cut(s)	45, 162, 632, 916, 1075, 1281, 1632, 1662, 1745
DpnII	GATC	9 cut(s)	43, 160, 630, 914, 1073, 1279, 1630, 1660, 1743
Eam1104I	CTCTTC	2 cut(s)	79, 1221
EarI	CTCTTC	2 cut(s)	79, 1221
Ecl136II	GAGCTC	1 cut(s)	463
Eco130I	CCWWGG	2 cut(s)	908, 928
Eco24I	GRGCYC	2 cut(s)	465, 2086
Eco31I	GGTCTC	1 cut(s)	231
Eco47I	GGWCC	1 cut(s)	1288
Eco53kI	GAGCTC	1 cut(s)	463
Eco57I	CTGAAG	1 cut(s)	1434
Eco81I	CCTNAGG	1 cut(s)	184
EcoICRI	GAGCTC	1 cut(s)	463
EcoO109I	RGGNCCY	1 cut(s)	421
EcoRI	GAATTC	1 cut(s)	2015
EcoRII	CCWGG	1 cut(s)	221
EcoT14I	CCWWGG	2 cut(s)	908, 928
EcoT22I	ATGCAT	1 cut(s)	1626
EcoT38I	GRGCYC	2 cut(s)	465, 2086
ErhI	CCWWGG	2 cut(s)	908, 928
FaeI	CATG	6 cut(s)	584, 598, 618, 869, 1611, 1829
FalI	AAGNNNNNCTT	4 cut(s)	984, 1016, 1432, 1464
FaqI	GGGAC	1 cut(s)	1274
FatI	CATG	6 cut(s)	580, 594, 614, 865, 1607, 1825
FauNDI	CATATG	1 cut(s)	1731
FbaI	TGATCA	1 cut(s)	1743
FblI	GTMKAC	1 cut(s)	2167
FriOI	GRGCYC	2 cut(s)	465, 2086
FspBI	CTAG	3 cut(s)	26, 1344, 2145
FspI	TGCGCA	1 cut(s)	2054
GlaI	GCGC	1 cut(s)	2054
GsaI	CCCAGC	1 cut(s)	153
GsuI	CTGGAG	2 cut(s)	491, 1877
HaeIII	GGCC	6 cut(s)	423, 839, 1016, 1507, 1613, 2138
HapII	CCGG	7 cut(s)	39, 128, 266, 276, 304, 545, 1552
HgaI	GACGC	2 cut(s)	1837, 2099
HhaI	GCGC	1 cut(s)	2055
Hin1I	GRCGYC	1 cut(s)	1848
Hin1II	CATG	6 cut(s)	584, 598, 618, 869, 1611, 1829
Hin6I	GCGC	1 cut(s)	2053
HinP1I	GCGC	1 cut(s)	2053
HincII	GTYRAC	3 cut(s)	732, 1093, 1266
HindII	GTYRAC	3 cut(s)	732, 1093, 1266
HpaII	CCGG	7 cut(s)	39, 128, 266, 276, 304, 545, 1552
HphI	GGTGA	2 cut(s)	172, 1334
Hpy166II	GTNNAC	7 cut(s)	301, 625, 732, 1093, 1266, 2043, 2168
Hpy8I	GTNNAC	7 cut(s)	301, 625, 732, 1093, 1266, 2043, 2168
Hpy99I	CGWCG	1 cut(s)	59
HpyAV	CCTTC	5 cut(s)	148, 644, 1374, 1686, 2137
HpyCH4III	ACNGT	3 cut(s)	412, 1008, 2202
HpyCH4IV	ACGT	5 cut(s)	557, 724, 805, 943, 1144
HpyF10VI	GCNNNNNNNGC	9 cut(s)	39, 448, 460, 550, 577, 836, 861, 1112, 1696
HpySE526I	ACGT	5 cut(s)	557, 724, 805, 943, 1144
Hsp92I	GRCGYC	1 cut(s)	1848
Hsp92II	CATG	6 cut(s)	584, 598, 618, 869, 1611, 1829
HspAI	GCGC	1 cut(s)	2053
Kpn2I	TCCGGA	1 cut(s)	127
Ksp22I	TGATCA	1 cut(s)	1743
Kzo9I	GATC	9 cut(s)	43, 160, 630, 914, 1073, 1279, 1630, 1660, 1743
LmnI	GCTCC	1 cut(s)	130
Lsp1109I	GCAGC	7 cut(s)	492, 623, 771, 1099, 1141, 1291, 1708
LweI	GCATC	5 cut(s)	518, 1233, 1350, 1633, 2097
MabI	ACCWGGT	1 cut(s)	221
MaeI	CTAG	3 cut(s)	26, 1344, 2145
MaeII	ACGT	5 cut(s)	557, 724, 805, 943, 1144
MaeIII	GTNAC	3 cut(s)	15, 178, 412
MalI	GATC	9 cut(s)	45, 162, 632, 916, 1075, 1281, 1632, 1662, 1745
MboI	GATC	9 cut(s)	43, 160, 630, 914, 1073, 1279, 1630, 1660, 1743
MfeI	CAATTG	1 cut(s)	1238
MflI	RGATCY	4 cut(s)	160, 630, 914, 1073
MhlI	GDGCHC	4 cut(s)	69, 465, 1539, 2086
MlyI	GAGTC	7 cut(s)	83, 271, 398, 409, 698, 1088, 2083
MmeI	TCCRAC	2 cut(s)	126, 169
Mph1103I	ATGCAT	1 cut(s)	1626
MroI	TCCGGA	1 cut(s)	127
MroXI	GAANNNNTTC	1 cut(s)	999
MseI	TTAA	4 cut(s)	1311, 1383, 1833, 1865
MslI	CAYNNNNRTG	1 cut(s)	1656
MspA1I	CMGCKG	3 cut(s)	454, 505, 1300
MspCI	CTTAAG	1 cut(s)	1310
MspI	CCGG	7 cut(s)	39, 128, 266, 276, 304, 545, 1552
MspR9I	CCNGG	2 cut(s)	223, 1553
MunI	CAATTG	1 cut(s)	1238
Mva1269I	GAATGC	2 cut(s)	561, 1941
MvaI	CCWGG	1 cut(s)	223
MwoI	GCNNNNNNNGC	9 cut(s)	39, 448, 460, 550, 577, 836, 861, 1112, 1696
NciI	CCSGG	1 cut(s)	1553
NdeI	CATATG	1 cut(s)	1731
NdeII	GATC	9 cut(s)	43, 160, 630, 914, 1073, 1279, 1630, 1660, 1743
NlaIII	CATG	6 cut(s)	584, 598, 618, 869, 1611, 1829
NlaIV	GGNNCC	4 cut(s)	396, 632, 906, 1290
NmeAIII	GCCGAG	2 cut(s)	285, 318
NmuCI	GTSAC	2 cut(s)	178, 412
NsbI	TGCGCA	1 cut(s)	2054
NsiI	ATGCAT	1 cut(s)	1626
NspI	RCATGY	1 cut(s)	584
PaeI	GCATGC	1 cut(s)	584
PagI	TCATGA	1 cut(s)	1825
PaqCI	CACCTGC	1 cut(s)	892
PcsI	WCGNNNNNNNCGW	2 cut(s)	744, 1660
PctI	GAATGC	2 cut(s)	561, 1941
PdmI	GAANNNNTTC	1 cut(s)	999
PfeI	GAWTC	9 cut(s)	84, 174, 334, 511, 815, 1052, 1784, 1820, 2194
PleI	GAGTC	7 cut(s)	83, 270, 397, 409, 697, 1088, 2082
PpsI	GAGTC	7 cut(s)	83, 270, 397, 409, 697, 1088, 2082
Ppu21I	YACGTR	3 cut(s)	725, 806, 1145
Psp124BI	GAGCTC	1 cut(s)	465
Psp6I	CCWGG	1 cut(s)	221
PspFI	CCCAGC	1 cut(s)	149
PspGI	CCWGG	1 cut(s)	221
PspN4I	GGNNCC	4 cut(s)	396, 632, 906, 1290
PspPI	GGNCC	4 cut(s)	421, 1288, 1506, 1611
PsrI	GAACNNNNNNTAC	2 cut(s)	2024, 2056
PstI	CTGCAG	1 cut(s)	2182
PstNI	CAGNNNCTG	3 cut(s)	508, 1430, 2186
PsuI	RGATCY	4 cut(s)	160, 630, 914, 1073
PvuII	CAGCTG	2 cut(s)	505, 1300
RsaI	GTAC	9 cut(s)	270, 302, 599, 626, 1143, 1404, 1547, 1862, 2042
RsaNI	GTAC	9 cut(s)	269, 301, 598, 625, 1142, 1403, 1546, 1861, 2041
RseI	CAYNNNNRTG	1 cut(s)	1656
SacI	GAGCTC	1 cut(s)	465
SaqAI	TTAA	4 cut(s)	1311, 1383, 1833, 1865
Sau3AI	GATC	9 cut(s)	43, 160, 630, 914, 1073, 1279, 1630, 1660, 1743
Sau96I	GGNCC	4 cut(s)	421, 1288, 1506, 1611
ScaI	AGTACT	2 cut(s)	1547, 1862
SchI	GAGTC	7 cut(s)	83, 271, 398, 409, 698, 1088, 2083
ScrFI	CCNGG	2 cut(s)	223, 1553
SduI	GDGCHC	4 cut(s)	69, 465, 1539, 2086
SexAI	ACCWGGT	1 cut(s)	221
SfaNI	GCATC	5 cut(s)	518, 1233, 1350, 1633, 2097
SfcI	CTRYAG	2 cut(s)	1317, 2178
SgrAI	CRCCGGYG	1 cut(s)	38
SinI	GGWCC	1 cut(s)	1288
SmiMI	CAYNNNNRTG	1 cut(s)	1656
SmlI	CTYRAG	3 cut(s)	1310, 1354, 1577
SmoI	CTYRAG	3 cut(s)	1310, 1354, 1577
SpeI	ACTAGT	1 cut(s)	25
SphI	GCATGC	1 cut(s)	584
SsiI	CCGC	8 cut(s)	211, 356, 449, 452, 495, 498, 1234, 1246
SspI	AATATT	1 cut(s)	1565
SspMI	CTAG	3 cut(s)	26, 1344, 2145
SstI	GAGCTC	1 cut(s)	465
StyD4I	CCNGG	2 cut(s)	221, 1551
StyI	CCWWGG	2 cut(s)	908, 928
TaaI	ACNGT	3 cut(s)	412, 1008, 2202
TaiI	ACGT	5 cut(s)	560, 727, 808, 946, 1147
TaqI	TCGA	4 cut(s)	54, 87, 874, 2030
TatI	WGTACW	4 cut(s)	597, 1545, 1860, 2040
TauI	GCSGC	5 cut(s)	451, 454, 498, 1236, 1249
TfiI	GAWTC	9 cut(s)	84, 174, 334, 511, 815, 1052, 1784, 1820, 2194
Tru1I	TTAA	4 cut(s)	1311, 1383, 1833, 1865
Tru9I	TTAA	4 cut(s)	1311, 1383, 1833, 1865
TscAI	CASTG	5 cut(s)	1179, 1220, 1435, 1984, 2187
TseFI	GTSAC	2 cut(s)	178, 412
TseI	GCWGC	7 cut(s)	505, 611, 784, 1112, 1129, 1304, 1696
Tsp45I	GTSAC	2 cut(s)	178, 412
TspDTI	ATGAA	5 cut(s)	130, 649, 755, 1356, 1946
TspGWI	ACGGA	3 cut(s)	336, 663, 749
TspRI	CASTG	5 cut(s)	1179, 1220, 1435, 1984, 2187
Vha464I	CTTAAG	1 cut(s)	1310
VpaK11BI	GGWCC	1 cut(s)	1288
XapI	RAATTY	3 cut(s)	232, 1420, 2015
XbaI	TCTAGA	1 cut(s)	1343
XceI	RCATGY	1 cut(s)	584
XcmI	CCANNNNNNNNNTGG	1 cut(s)	325
XmiI	GTMKAC	1 cut(s)	2167
XmnI	GAANNNNTTC	1 cut(s)	999
XspI	CTAG	3 cut(s)	26, 1344, 2145
ZrmI	AGTACT	2 cut(s)	1547, 1862
Zsp2I	ATGCAT	1 cut(s)	1626

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.