FvH4_2g26410

Auxin transporter-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014922
Physical Location & Seq
Forward (+)
21207919 .. 21210861
2943 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26410.t1

Sequence Viewer

Length: 1401 bp
ATGGCTTCCGAGAAGGTCGAGACCATTATTGCCGGAAGCTACTTAGAGATGGAAAGGGAAGATGGTGATGCCAAGGCTTCTGCTAAGAGCAAGCTATCGACGTTTCTCTGGCACGGCGGCTCCGCCTATGATGCATGGTTTAGCTGTGCTTCTAACCAGGTTGCACAAGTGCTTTTGACACTGCCGTACTCATTTTCACAGCTGGGTTTGTTATCTGGGATTCTATTTCAGCTTTTCTATGGATTGATGGGAAGCTGGACTGCTTACCTTATCAGTCTACTGTATATTGAGTACAGAACTAGAAAGGAAAGAGAAAAGGCCGATTTCAGAAACCACGTCATTCAGTGGTTTGAAGTTCTTGATGGACTTCTGGGAAAGCACTGGAGGAATTTAGGCCTCTTTTTCAACTGTACTTTTCTTCTGTTCGGATCTGTAATTCAGTTAATTGCTTGTGCAAGTAACATCTACTACATAAACGACAATCTTGACAAGAGAACTTGGACGTATATCTTTGGAGCTTGCTGTGCTACAACTGTCTTCATACCTTCATTTCATAATTACAGAATTTGGTCGTTCTTGGGTCTTATTATGACTACTTACACTGCATGGTATCTAACCATTGCTTCCCTTATCCATGGACAGGTTGAAGGAGTGAAGCACTCAGGTCCAACCACGATGGTTCTCTACTTCACTGGAGCCACCAACATTCTCTACACTTTTGGTGGACATGCTGTTACAGTAGAGATTATGCATGCAATGTGGAAGCCACAGAAATTCAAGATGATATACTTGATGGCAACACTATATGTGCTGACCTTAACTTTACCATCAGCCTCTGCTGTGTACTGGGCTTTTGGAGACGCCCTCTTGACTCATTCCAACGCTCTCGCTTTGCTTCCGAGGACCAGATTTAGAGACACTGCTGTTGTTCTCATGCTCATTCACCAGTTTATAACATTTGGGTTTGCTTGCACTCCATTGTACTTTGTGTGGGAGAAGTTCTTGAGGATTCATGAGACCAAAAGCATGTTGAAGAGGGCCATTGCTAGACTTCCAGTGGTGATCCCAATTTGGTTCCTTGCAATCATATTCCCATTCTTTGGCCCCATCAACTCAACTGTTGGATCTCTTCTTGTCAGCTTTACTGTCTACATAATCCCTGCTTTAGCTCACATGGTCACTTTTGCTTCTCCACAGGCGAGAGAGAATGCTGTCGAGAGACCACCATCATTCTTAGGAGGGTGGCCAGGATCATACACCATGAACATCTTTGTGGTGGTGTGGGTGGCAATAGTGGGATTTGGATTTGGAGGGTGGGCAAGCATGCTCAACTTCATACACCAAGTTGATACATTTGGTCTATTCACTAAGTGCTACCAATGCCCACCTCACAAAGCTTGA

Protein Analysis

467

Amino Acids

52.75

Weight (kDa)

8.95

Isoelectric Point (pI)

30.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 38 - 428 4.3e-80 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014922)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77690
fragaria_vesca FvH4_2g26410
malus_domestica MD15G1355600.v1.1
prunus_persica Prupe.1G503100_v2.0.a1
pyrus_communis pycom08g14440 pycom15g31780
rosa_chinensis RchiOBHm_Chr6g0295041
rosa_laevigata RLG00000011824
rosa_multiflora Rmu_sc0008767.1_g000008
rosa_roxburghii Rroxscaffold_7G00172780
rosa_rugosa Rorug06G0249000
rosa_samantha Rh6AG362000 Rh6BG369600 Rh6CG376200 Rh6DG362800
rosa_wichuraiana Rw6G031590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 953
AccB7I CCANNNNNTGG 1 cut(s) 1100
AccI GTMKAC 2 cut(s) 277, 1149
AciI CCGC 2 cut(s) 117, 123
AclWI GGATC 4 cut(s) 436, 1057, 1132, 1258
AcoI YGGCCR 1 cut(s) 1244
AcsI RAATTY 3 cut(s) 388, 564, 773
AcyI GRCGYC 1 cut(s) 861
AdeI CACNNNGTG 1 cut(s) 1371
AfaI GTAC 5 cut(s) 188, 293, 412, 845, 983
AfiI CCNNNNNNNGG 2 cut(s) 640, 1100
AgsI TTSAA 5 cut(s) 353, 406, 647, 778, 1033
AjiI CACGTC 1 cut(s) 337
AjnI CCWGG 2 cut(s) 156, 1246
Alw26I GTCTC 5 cut(s) 14, 852, 909, 1010, 1213
AlwI GGATC 4 cut(s) 436, 1057, 1132, 1258
AlwNI CAGNNNCTG 1 cut(s) 836
AoxI GGCC 5 cut(s) 318, 394, 1038, 1102, 1244
ApoI RAATTY 3 cut(s) 388, 564, 773
AspS9I GGNCC 4 cut(s) 665, 903, 1038, 1103
AsuHPI GGTGA 3 cut(s) 77, 935, 1072
AvaII GGWCC 2 cut(s) 665, 903
BalI TGGCCA 1 cut(s) 1246
BbsI GAAGAC 1 cut(s) 529
BccI CCATC 9 cut(s) 43, 56, 241, 356, 670, 787, 835, 1115, 1234
BceAI ACGGC 2 cut(s) 130, 169
BciT130I CCWGG 2 cut(s) 158, 1248
BcoDI GTCTC 5 cut(s) 14, 852, 909, 1010, 1213
BfaI CTAG 2 cut(s) 300, 1047
BisI GCNGC 1 cut(s) 118
BlsI GCNGC 1 cut(s) 119
Bme1390I CCNGG 2 cut(s) 158, 1248
Bme18I GGWCC 2 cut(s) 665, 903
BmgBI CACGTC 1 cut(s) 337
BmgT120I GGNCC 4 cut(s) 665, 903, 1038, 1103
BmiI GGNNCC 4 cut(s) 121, 697, 1076, 1105
BmrFI CCNGG 2 cut(s) 158, 1248
BmrI ACTGGG 1 cut(s) 856
BmsI GCATC 2 cut(s) 58, 121
BmuI ACTGGG 1 cut(s) 856
BpiI GAAGAC 1 cut(s) 529
BplI GAGNNNNNCTC 2 cut(s) 849, 881
BpmI CTGGAG 2 cut(s) 403, 714
BpuEI CTTGAG 1 cut(s) 1024
BsaHI GRCGYC 1 cut(s) 861
BsaI GGTCTC 3 cut(s) 14, 1010, 1213
BsaJI CCNNGG 3 cut(s) 72, 634, 899
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 2 cut(s) 640, 1100
Bse1I ACTGG 5 cut(s) 386, 697, 851, 946, 1055
Bse3DI GCAATG 3 cut(s) 618, 762, 1041
BseBI CCWGG 2 cut(s) 158, 1248
BseDI CCNNGG 3 cut(s) 72, 634, 899
BseLI CCNNNNNNNGG 2 cut(s) 640, 1100
BseMI GCAATG 3 cut(s) 618, 762, 1041
BseMII CTCAG 1 cut(s) 675
BseNI ACTGG 5 cut(s) 386, 697, 851, 946, 1055
BseYI CCCAGC 1 cut(s) 202
BshFI GGCC 5 cut(s) 320, 396, 1040, 1104, 1246
BsiSI CCGG 1 cut(s) 33
BslI CCNNNNNNNGG 2 cut(s) 640, 1100
BsmAI GTCTC 5 cut(s) 14, 852, 909, 1010, 1213
BsmBI CGTCTC 1 cut(s) 852
BsmI GAATGC 1 cut(s) 1213
BsnI GGCC 5 cut(s) 320, 396, 1040, 1104, 1246
Bso31I GGTCTC 3 cut(s) 14, 1010, 1213
Bsp143I GATC 4 cut(s) 428, 1062, 1124, 1250
Bsp19I CCATGG 1 cut(s) 634
BspACI CCGC 2 cut(s) 117, 123
BspANI GGCC 5 cut(s) 320, 396, 1040, 1104, 1246
BspCNI CTCAG 1 cut(s) 674
BspHI TCATGA 1 cut(s) 1012
BspLI GGNNCC 4 cut(s) 121, 697, 1076, 1105
BspPI GGATC 4 cut(s) 436, 1057, 1132, 1258
BspTNI GGTCTC 3 cut(s) 14, 1010, 1213
BsrDI GCAATG 3 cut(s) 618, 762, 1041
BsrI ACTGG 5 cut(s) 386, 697, 851, 946, 1055
BssECI CCNNGG 3 cut(s) 72, 634, 899
BssMI GATC 4 cut(s) 428, 1062, 1124, 1250
BssNI GRCGYC 1 cut(s) 861
BssT1I CCWWGG 2 cut(s) 72, 634
Bst2UI CCWGG 2 cut(s) 158, 1248
Bst4CI ACNGT 6 cut(s) 282, 410, 535, 739, 1120, 1147
Bst6I CTCTTC 2 cut(s) 1028, 1134
BstACI GRCGYC 1 cut(s) 861
BstC8I GCNNGC 6 cut(s) 92, 520, 753, 970, 1321, 1325
BstDEI CTNAG 5 cut(s) 43, 84, 661, 1234, 1368
BstDSI CCRYGG 1 cut(s) 634
BstKTI GATC 4 cut(s) 431, 1065, 1127, 1253
BstMAI GTCTC 5 cut(s) 14, 852, 909, 1010, 1213
BstMBI GATC 4 cut(s) 428, 1062, 1124, 1250
BstMWI GCNNNNNNNGC 3 cut(s) 131, 524, 1380
BstNI CCWGG 2 cut(s) 158, 1248
BstNSI RCATGY 4 cut(s) 731, 755, 1030, 1327
BstSCI CCNGG 2 cut(s) 156, 1246
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 2 cut(s) 428, 1124
BstYI RGATCY 2 cut(s) 428, 1124
BsuRI GGCC 5 cut(s) 320, 396, 1040, 1104, 1246
BtgI CCRYGG 1 cut(s) 634
BtrI CACGTC 1 cut(s) 337
BtsI GCAGTG 3 cut(s) 179, 600, 918
BtsIMutI CAGTG 7 cut(s) 179, 350, 379, 600, 690, 918, 1062
Cac8I GCNNGC 6 cut(s) 92, 520, 753, 970, 1321, 1325
CaiI CAGNNNCTG 1 cut(s) 836
CciI TCATGA 1 cut(s) 1012
Cfr13I GGNCC 4 cut(s) 665, 903, 1038, 1103
CseI GACGC 1 cut(s) 869
CsiI ACCWGGT 1 cut(s) 156
Csp6I GTAC 5 cut(s) 187, 292, 411, 844, 982
CviQI GTAC 5 cut(s) 187, 292, 411, 844, 982
DdeI CTNAG 5 cut(s) 43, 84, 661, 1234, 1368
DpnI GATC 4 cut(s) 430, 1064, 1126, 1252
DpnII GATC 4 cut(s) 428, 1062, 1124, 1250
DraIII CACNNNGTG 1 cut(s) 1371
EaeI YGGCCR 1 cut(s) 1244
Eam1104I CTCTTC 2 cut(s) 1028, 1134
EarI CTCTTC 2 cut(s) 1028, 1134
EciI GGCGGA 1 cut(s) 112
Eco130I CCWWGG 2 cut(s) 72, 634
Eco147I AGGCCT 1 cut(s) 396
Eco31I GGTCTC 3 cut(s) 14, 1010, 1213
Eco47I GGWCC 2 cut(s) 665, 903
EcoRII CCWGG 2 cut(s) 156, 1246
EcoT14I CCWWGG 2 cut(s) 72, 634
EcoT22I ATGCAT 2 cut(s) 136, 753
ErhI CCWWGG 2 cut(s) 72, 634
Esp3I CGTCTC 1 cut(s) 852
FblI GTMKAC 2 cut(s) 277, 1149
Fnu4HI GCNGC 1 cut(s) 118
Fsp4HI GCNGC 1 cut(s) 118
FspBI CTAG 2 cut(s) 300, 1047
GluI GCNGC 1 cut(s) 118
GsaI CCCAGC 1 cut(s) 206
GsuI CTGGAG 2 cut(s) 403, 714
HaeIII GGCC 5 cut(s) 320, 396, 1040, 1104, 1246
HapII CCGG 1 cut(s) 33
HgaI GACGC 1 cut(s) 869
Hin1I GRCGYC 1 cut(s) 861
HindIII AAGCTT 1 cut(s) 1395
HinfI GANTC 3 cut(s) 220, 871, 1009
HpaII CCGG 1 cut(s) 33
HphI GGTGA 3 cut(s) 77, 935, 1072
Hpy166II GTNNAC 4 cut(s) 278, 725, 844, 1150
Hpy188I TCNGA 4 cut(s) 10, 329, 428, 900
Hpy188III TCNNGA 8 cut(s) 19, 359, 485, 778, 868, 1003, 1013, 1216
Hpy8I GTNNAC 4 cut(s) 278, 725, 844, 1150
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 3 cut(s) 7, 555, 641
HpyCH4III ACNGT 6 cut(s) 282, 410, 535, 739, 1120, 1147
HpyCH4IV ACGT 3 cut(s) 101, 336, 503
HpyCH4V TGCA 8 cut(s) 134, 164, 455, 605, 751, 755, 972, 1082
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 524, 1380
HpyF3I CTNAG 5 cut(s) 43, 84, 661, 1234, 1368
HpySE526I ACGT 3 cut(s) 101, 336, 503
Hsp92I GRCGYC 1 cut(s) 861
Kzo9I GATC 4 cut(s) 428, 1062, 1124, 1250
LmnI GCTCC 3 cut(s) 125, 515, 695
LweI GCATC 2 cut(s) 58, 121
MabI ACCWGGT 1 cut(s) 156
MaeI CTAG 2 cut(s) 300, 1047
MaeII ACGT 3 cut(s) 101, 336, 503
MaeIII GTNAC 3 cut(s) 458, 733, 1177
MalI GATC 4 cut(s) 430, 1064, 1126, 1252
MboI GATC 4 cut(s) 428, 1062, 1124, 1250
MboII GAAGA 5 cut(s) 71, 410, 529, 1045, 1121
MflI RGATCY 2 cut(s) 428, 1124
MlsI TGGCCA 1 cut(s) 1246
MluCI AATT 7 cut(s) 388, 435, 444, 556, 564, 773, 1068
MluNI TGGCCA 1 cut(s) 1246
MlyI GAGTC 1 cut(s) 865
MmeI TCCRAC 3 cut(s) 692, 903, 1102
Mox20I TGGCCA 1 cut(s) 1246
Mph1103I ATGCAT 2 cut(s) 136, 753
MscI TGGCCA 1 cut(s) 1246
MseI TTAA 2 cut(s) 443, 818
MslI CAYNNNNRTG 1 cut(s) 1271
Msp20I TGGCCA 1 cut(s) 1246
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 2 cut(s) 158, 1248
Mva1269I GAATGC 1 cut(s) 1213
MvaI CCWGG 2 cut(s) 158, 1248
MwoI GCNNNNNNNGC 3 cut(s) 131, 524, 1380
NcoI CCATGG 1 cut(s) 634
NdeII GATC 4 cut(s) 428, 1062, 1124, 1250
NlaIV GGNNCC 4 cut(s) 121, 697, 1076, 1105
NmuCI GTSAC 1 cut(s) 1177
NsiI ATGCAT 2 cut(s) 136, 753
NspI RCATGY 4 cut(s) 731, 755, 1030, 1327
PaeI GCATGC 2 cut(s) 755, 1327
PagI TCATGA 1 cut(s) 1012
PceI AGGCCT 1 cut(s) 396
PctI GAATGC 1 cut(s) 1213
PfeI GAWTC 2 cut(s) 220, 1009
PflMI CCANNNNNTGG 1 cut(s) 1100
PkrI GCNGC 1 cut(s) 119
PleI GAGTC 1 cut(s) 865
PpsI GAGTC 1 cut(s) 865
PsiI TTATAA 1 cut(s) 953
Psp6I CCWGG 2 cut(s) 156, 1246
PspFI CCCAGC 1 cut(s) 202
PspGI CCWGG 2 cut(s) 156, 1246
PspN4I GGNNCC 4 cut(s) 121, 697, 1076, 1105
PspPI GGNCC 4 cut(s) 665, 903, 1038, 1103
PstNI CAGNNNCTG 1 cut(s) 836
PsuI RGATCY 2 cut(s) 428, 1124
PvuII CAGCTG 1 cut(s) 202
RsaI GTAC 5 cut(s) 188, 293, 412, 845, 983
RsaNI GTAC 5 cut(s) 187, 292, 411, 844, 982
RseI CAYNNNNRTG 1 cut(s) 1271
SaqAI TTAA 2 cut(s) 443, 818
SatI GCNGC 1 cut(s) 118
Sau3AI GATC 4 cut(s) 428, 1062, 1124, 1250
Sau96I GGNCC 4 cut(s) 665, 903, 1038, 1103
SchI GAGTC 1 cut(s) 865
ScrFI CCNGG 2 cut(s) 158, 1248
SexAI ACCWGGT 1 cut(s) 156
SfaNI GCATC 2 cut(s) 58, 121
SinI GGWCC 2 cut(s) 665, 903
SmiMI CAYNNNNRTG 1 cut(s) 1271
SmlI CTYRAG 1 cut(s) 1003
SmoI CTYRAG 1 cut(s) 1003
SphI GCATGC 2 cut(s) 755, 1327
Sse9I AATT 7 cut(s) 388, 435, 444, 556, 564, 773, 1068
SseBI AGGCCT 1 cut(s) 396
SsiI CCGC 2 cut(s) 117, 123
SspMI CTAG 2 cut(s) 300, 1047
StuI AGGCCT 1 cut(s) 396
StyD4I CCNGG 2 cut(s) 156, 1246
StyI CCWWGG 2 cut(s) 72, 634
TaaI ACNGT 6 cut(s) 282, 410, 535, 739, 1120, 1147
TaiI ACGT 3 cut(s) 104, 339, 506
TaqI TCGA 3 cut(s) 18, 98, 1215
TasI AATT 7 cut(s) 388, 435, 444, 556, 564, 773, 1068
TatI WGTACW 4 cut(s) 291, 410, 843, 981
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 2 cut(s) 220, 1009
Tru1I TTAA 2 cut(s) 443, 818
Tru9I TTAA 2 cut(s) 443, 818
TscAI CASTG 7 cut(s) 186, 350, 386, 607, 697, 925, 1062
TseFI GTSAC 1 cut(s) 1177
Tsp45I GTSAC 1 cut(s) 1177
TspDTI ATGAA 6 cut(s) 529, 537, 542, 1001, 1277, 1324
TspRI CASTG 7 cut(s) 186, 350, 386, 607, 697, 925, 1062
Van91I CCANNNNNTGG 1 cut(s) 1100
VpaK11BI GGWCC 2 cut(s) 665, 903
XapI RAATTY 3 cut(s) 388, 564, 773
XceI RCATGY 4 cut(s) 731, 755, 1030, 1327
XmiI GTMKAC 2 cut(s) 277, 1149
XspI CTAG 2 cut(s) 300, 1047
Zsp2I ATGCAT 2 cut(s) 136, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.