Rorug06G0249000
MADS Family

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000249
Physical Location & Seq
Reverse (-)
39541161 .. 39544419
3259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0249000.1

Sequence Viewer

Length: 1158 bp
ATGGCTGCAGGGACAGATACCCCATCTTCACTTTCCATGTTAGATGGCAGTTACAGAAAGAACGTAACTGTTACAAGGGACGAGAAATCAAATACTTTTGAGAACATGAAGCAATCCAACATCGGCAAACCTCCTAGGCATCTTTCAGCTATTAGGAACTGTGTCAGCTCTGCCCAGTTGGCTCCGGCAACTGAAGTGGAGTTCGATAGTGGGATTGTTAGCTCAAAATCCTCGTCAGATAGTAATTCACCATTTGTACCTATTTTTCGGTCCGGAAGCTGTTCAGAGATTGGACCGAAACAATTTATGGAGGATGAGCATATCTGTATAGACAATATTCTTGAACATCTAGGATCTACTGCAGACTTTCCTTCTCCTGGAGCTTTCTATGGGGTTTTTGATGGCCATGGTGGTACAGATGCAGCTTCTTATATCAGAGAGAATATTTTGAGGTTTATAGTGGAGGATTCACATTTCCCTTTTTGTGCTAAGCAAGCTATTAAGAGTGCTTTTCTAAAGGCTGACCATGCTTTTGCTGATAATGGTACTCTTGATAGTTCCTCTGGTACCACTGCACTTACTGCCCTTATCTCTGGAAGGACGATGCTAGTTGCCAATGCTGGAGATTGTCGAGCTGTTCTGGGAAGAAGAGGCAGGGTGGTGGAACTGTCCAAAGACCATAAACCAAATTGTGCTTCAGAAAGAGTAAGAATTGAAGAACTGGGAGGCGTGGTGTATGATGGATACCTCAACGGCCAATTGTCAGTGGCACGAGCACTTGGAGATTGGCACATGAAAGGGTCCAAAGGTTCCTCTTGTCCCTTAAGTGCAGAACCGGAGCTTGAGGAGACAATTCTGAGTGAGGAAGATGAGTTCTTGATCATAGGTTGTGATGGACTGTGGGATGTGATGAGCAGCCAGTGTGCCGTCACAATTGCAAGGAAAGAATTGATGCTTCACAATGATCCTGAAAGATGCTCCAGAGAGCTAGTTAGAGAAGCTCTCAAGCGTGATACATGTGATAACTTGACCGTCGTAGTGGTATGCTTCTCCCCAGAACCACCGCCTCAGCTTGAGATCCCAAAGTTCAAGTTCAAGAGGAATATATCAGCAGAAGGTCTGAATCTTCTGCAGGAGGTACTAGATAGTAATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

385

Amino Acids

41.85

Weight (kDa)

5.12

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 92 - 342 1.9e-74 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22590 AT1G22590 AT2G28700 AT3G05860 AT3G05860 AT3G05860 AT5G26630 AT5G27810 AT5G48670
fragaria_vesca FvH4_6g08460 FvH4_6g08570 FvH4_6g21170 FvH4_6g43410 FvH4_7g09730
malus_domestica MD04G1186100.v1.1 MD04G1186200.v1.1 MD04G1186300.v1.1 MD06G1013100.v1.1 MD06G1013200.v1.1 MD06G1013500.v1.1 MD06G1013600.v1.1 MD08G1200100.v1.1 MD12G1199500.v1.1 MD12G1199600.v1.1 MD12G1199700.v1.1 MD15G1387300.v1.1
prunus_persica Prupe.1G533600_v2.0.a1 Prupe.1G533700_v2.0.a1 Prupe.3G020400_v2.0.a1 Prupe.6G303900_v2.0.a1 Prupe.6G304100_v2.0.a1 Prupe.7G092700_v2.0.a1
pyrus_communis pycom04g16460 pycom06g01090 pycom12g18580 pycom12g18590
rosa_chinensis RchiOBHm_Chr1g0321431 RchiOBHm_Chr1g0336291 RchiOBHm_Chr1g0346681 RchiOBHm_Chr1g0346741 RchiOBHm_Chr1g0347651 RchiOBHm_Chr3g0458611 RchiOBHm_Chr6g0267971 RchiOBHm_Chr6g0295031 RchiOBHm_Chr6g0296451 RchiOBHm_Chr7g0222461 RchiOBHm_Chr7g0222491 RchiOBHm_Chr7g0222511 RchiOBHm_Chr7g0223301
rosa_laevigata RLG00000002052 RLG00000002057 RLG00000011691 RLG00000025146 RLG00000028786 RLG00000029375 RLG00000030422
rosa_multiflora Rmu_co8262069.1_g000001 Rmu_sc0000087.1_g000025 Rmu_sc0000724.1_g000014 Rmu_sc0001453.1_g000004 Rmu_sc0001634.1_g000008 Rmu_sc0002096.1_g000035 Rmu_sc0002349.1_g000003 Rmu_sc0002349.1_g000024 Rmu_sc0003232.1_g000005 Rmu_sc0004404.1_g000010 Rmu_sc0004404.1_g000018 Rmu_sc0004638.1_g000024 Rmu_sc0005124.1_g000012 Rmu_sc0008767.1_g000014 Rmu_sc0014221.1_g000005 Rmu_sc0038216.1_g000002 Rmu_ssc0000146.1_g000019 Rmu_ssc0000146.1_g000023
rosa_roxburghii Rroxscaffold_1G00004980 Rroxscaffold_3G00236720 Rroxscaffold_3G00236940 Rroxscaffold_4G00308370 Rroxscaffold_4G00308400 Rroxscaffold_4G00315810 Rroxscaffold_6G00420920 Rroxscaffold_7G00171520 Rroxscaffold_7G00200280
rosa_rugosa Rorug01G0032700 Rorug01G0123500 Rorug01G0182200 Rorug01G0182400 Rorug01G0188300 Rorug01G0188500 Rorug03G0030200 Rorug06G0033600 Rorug06G0249000 Rorug06G0262500 Rorug07G0208800 Rorug07G0209700 Rorug07G0210100 Rorug07G0215900 Rorug07G0216000
rosa_samantha Rh1AG044400 Rh1AG144000 Rh1AG201000 Rh1BG042600 Rh1BG113600 Rh1BG167200 Rh1BG171100 Rh1CG135700 Rh1DG150600 Rh3BG091400 Rh6AG157800 Rh6AG373900 Rh6BG156100 Rh6BG159900 Rh6BG369500 Rh6BG382500 Rh6DG141300 Rh6DG145600 Rh6DG362600 Rh6DG375000 Rh7AG351000 Rh7AG351600 Rh7AG351800 Rh7BG342000 Rh7BG342400 Rh7CG368300 Rh7CG369000 Rh7CG369200
rosa_wichuraiana Rw0G015210 Rw0G021330 Rw1G003960 Rw1G016840 Rw3G007550 Rw6G013730 Rw6G032610 Rw7G029680 Rw7G030010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 566
AccB1I GGYRCC 1 cut(s) 566
AccIII TCCGGA 1 cut(s) 272
AciI CCGC 1 cut(s) 1064
AclWI GGATC 3 cut(s) 361, 959, 1072
AcoI YGGCCR 2 cut(s) 403, 754
AcuI CTGAAG 2 cut(s) 213, 681
AfaI GTAC 5 cut(s) 258, 415, 547, 568, 1140
AfiI CCNNNNNNNGG 1 cut(s) 377
AflII CTTAAG 1 cut(s) 823
AflIII ACRYGT 1 cut(s) 1016
AgsI TTSAA 4 cut(s) 344, 716, 1090, 1096
AjnI CCWGG 1 cut(s) 376
AjuI GAANNNNNNNTTGG 2 cut(s) 679, 711
AloI GAACNNNNNNTCC 2 cut(s) 857, 889
Alw21I GWGCWC 1 cut(s) 778
Alw26I GTCTC 1 cut(s) 842
AlwI GGATC 3 cut(s) 361, 959, 1072
Aor13HI TCCGGA 1 cut(s) 272
AoxI GGCC 2 cut(s) 403, 754
ApeKI GCWGC 3 cut(s) 5, 422, 915
ArsI GACNNNNNNTTYG 4 cut(s) 218, 250, 515, 547
Asp700I GAANNNNTTC 1 cut(s) 280
Asp718I GGTACC 1 cut(s) 566
AspA2I CCTAGG 1 cut(s) 134
AspS9I GGNCC 3 cut(s) 270, 293, 801
AsuHPI GGTGA 1 cut(s) 240
AvaII GGWCC 3 cut(s) 270, 293, 801
AvrII CCTAGG 1 cut(s) 134
BalI TGGCCA 1 cut(s) 405
BanI GGYRCC 1 cut(s) 566
BauI CACGAG 1 cut(s) 771
Bbv12I GWGCWC 1 cut(s) 778
BbvCI CCTCAGC 1 cut(s) 1068
BbvI GCAGC 2 cut(s) 434, 927
BccI CCATC 5 cut(s) 31, 38, 395, 734, 887
BceAI ACGGC 2 cut(s) 769, 911
BciT130I CCWGG 1 cut(s) 378
BciVI GTATCC 1 cut(s) 737
BclI TGATCA 1 cut(s) 879
BcoDI GTCTC 1 cut(s) 842
BfaI CTAG 5 cut(s) 135, 350, 608, 989, 1142
BfmI CTRYAG 3 cut(s) 6, 360, 1130
BfrI CTTAAG 1 cut(s) 823
BfuI GTATCC 1 cut(s) 737
BglI GCCNNNNNGGC 1 cut(s) 179
BisI GCNGC 3 cut(s) 6, 423, 916
BlnI CCTAGG 1 cut(s) 134
BlpI GCTNAGC 1 cut(s) 489
BlsI GCNGC 3 cut(s) 7, 424, 917
Bme1390I CCNGG 1 cut(s) 378
Bme18I GGWCC 3 cut(s) 270, 293, 801
BmgT120I GGNCC 3 cut(s) 270, 293, 801
BmiI GGNNCC 4 cut(s) 183, 568, 802, 811
BmrFI CCNGG 1 cut(s) 378
BmrI ACTGGG 2 cut(s) 169, 731
BmsI GCATC 5 cut(s) 148, 409, 594, 942, 965
BmuI ACTGGG 2 cut(s) 169, 731
BplI GAGNNNNNCTC 2 cut(s) 987, 1019
BpmI CTGGAG 3 cut(s) 399, 642, 964
Bpu10I CCTNAGC 1 cut(s) 1068
Bpu1102I GCTNAGC 1 cut(s) 489
BpuEI CTTGAG 3 cut(s) 863, 989, 1094
BsaJI CCNNGG 2 cut(s) 134, 406
BsaWI WCCGGW 2 cut(s) 272, 835
BsaXI ACNNNNNCTCC 2 cut(s) 717, 747
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 3 cut(s) 175, 726, 919
BseAI TCCGGA 1 cut(s) 272
BseBI CCWGG 1 cut(s) 378
BseDI CCNNGG 2 cut(s) 134, 406
BseGI GGATG 2 cut(s) 319, 910
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMII CTCAG 2 cut(s) 848, 1082
BseNI ACTGG 3 cut(s) 175, 726, 919
BseRI GAGGAG 1 cut(s) 860
BseXI GCAGC 2 cut(s) 434, 927
BsgI GTGCAG 2 cut(s) 558, 849
BshFI GGCC 2 cut(s) 405, 756
BshNI GGYRCC 1 cut(s) 566
BsiHKAI GWGCWC 1 cut(s) 778
BsiSI CCGG 3 cut(s) 185, 273, 836
BslFI GGGAC 3 cut(s) 25, 92, 804
BslI CCNNNNNNNGG 1 cut(s) 377
BsmAI GTCTC 1 cut(s) 842
BsmFI GGGAC 3 cut(s) 25, 92, 804
BsnI GGCC 2 cut(s) 405, 756
Bsp1286I GDGCHC 1 cut(s) 778
Bsp13I TCCGGA 1 cut(s) 272
Bsp143I GATC 4 cut(s) 353, 879, 964, 1077
Bsp1720I GCTNAGC 1 cut(s) 489
Bsp19I CCATGG 1 cut(s) 406
BspACI CCGC 1 cut(s) 1064
BspANI GGCC 2 cut(s) 405, 756
BspCNI CTCAG 2 cut(s) 849, 1081
BspEI TCCGGA 1 cut(s) 272
BspLI GGNNCC 4 cut(s) 183, 568, 802, 811
BspMAI CTGCAG 3 cut(s) 10, 364, 1134
BspPI GGATC 3 cut(s) 361, 959, 1072
BspT107I GGYRCC 1 cut(s) 566
BspTI CTTAAG 1 cut(s) 823
BsrI ACTGG 3 cut(s) 175, 726, 919
BssECI CCNNGG 2 cut(s) 134, 406
BssMI GATC 4 cut(s) 353, 879, 964, 1077
BssSI CACGAG 1 cut(s) 771
BssT1I CCWWGG 2 cut(s) 134, 406
Bst2BI CACGAG 1 cut(s) 771
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 5 cut(s) 70, 161, 669, 900, 1033
Bst6I CTCTTC 1 cut(s) 643
BstAFI CTTAAG 1 cut(s) 823
BstAPI GCANNNNNTGC 1 cut(s) 581
BstC8I GCNNGC 1 cut(s) 495
BstDEI CTNAG 3 cut(s) 489, 857, 1068
BstDSI CCRYGG 1 cut(s) 406
BstF5I GGATG 2 cut(s) 319, 910
BstKTI GATC 4 cut(s) 356, 882, 967, 1080
BstMAI GTCTC 1 cut(s) 842
BstMBI GATC 4 cut(s) 353, 879, 964, 1077
BstMWI GCNNNNNNNGC 4 cut(s) 179, 494, 527, 581
BstNI CCWGG 1 cut(s) 378
BstNSI RCATGY 1 cut(s) 1020
BstSCI CCNGG 1 cut(s) 376
BstSFI CTRYAG 3 cut(s) 6, 360, 1130
BstV1I GCAGC 2 cut(s) 434, 927
BstX2I RGATCY 2 cut(s) 353, 1077
BstYI RGATCY 2 cut(s) 353, 1077
BsuI GTATCC 1 cut(s) 737
BsuRI GGCC 2 cut(s) 405, 756
BtgI CCRYGG 1 cut(s) 406
BtsCI GGATG 2 cut(s) 319, 910
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 3 cut(s) 570, 771, 926
Cac8I GCNNGC 1 cut(s) 495
Cfr13I GGNCC 3 cut(s) 270, 293, 801
CpoI CGGWCCG 1 cut(s) 270
Csp6I GTAC 5 cut(s) 257, 414, 546, 567, 1139
CspCI CAANNNNNGTGG 2 cut(s) 177, 212
CspI CGGWCCG 1 cut(s) 270
CviAII CATG 6 cut(s) 37, 106, 407, 527, 793, 1017
CviQI GTAC 5 cut(s) 257, 414, 546, 567, 1139
DdeI CTNAG 3 cut(s) 489, 857, 1068
DpnI GATC 4 cut(s) 355, 881, 966, 1079
DpnII GATC 4 cut(s) 353, 879, 964, 1077
EaeI YGGCCR 2 cut(s) 403, 754
Eam1104I CTCTTC 1 cut(s) 643
EarI CTCTTC 1 cut(s) 643
Eco130I CCWWGG 2 cut(s) 134, 406
Eco47I GGWCC 3 cut(s) 270, 293, 801
Eco57I CTGAAG 2 cut(s) 213, 681
EcoRII CCWGG 1 cut(s) 376
EcoT14I CCWWGG 2 cut(s) 134, 406
ErhI CCWWGG 2 cut(s) 134, 406
FaeI CATG 6 cut(s) 40, 109, 410, 530, 796, 1020
FaqI GGGAC 3 cut(s) 25, 92, 804
FatI CATG 6 cut(s) 36, 105, 406, 526, 792, 1016
FbaI TGATCA 1 cut(s) 879
Fnu4HI GCNGC 3 cut(s) 6, 423, 916
FokI GGATG 2 cut(s) 326, 917
Fsp4HI GCNGC 3 cut(s) 6, 423, 916
FspBI CTAG 5 cut(s) 135, 350, 608, 989, 1142
GluI GCNGC 3 cut(s) 6, 423, 916
GsuI CTGGAG 3 cut(s) 399, 642, 964
HaeIII GGCC 2 cut(s) 405, 756
HapII CCGG 3 cut(s) 185, 273, 836
Hin1II CATG 6 cut(s) 40, 109, 410, 530, 796, 1020
HinfI GANTC 2 cut(s) 467, 1123
HpaII CCGG 3 cut(s) 185, 273, 836
HphI GGTGA 1 cut(s) 240
Hpy188I TCNGA 6 cut(s) 238, 286, 437, 700, 858, 1122
Hpy188III TCNNGA 8 cut(s) 273, 341, 551, 594, 877, 968, 981, 1096
Hpy99I CGWCG 1 cut(s) 1037
HpyAV CCTTC 3 cut(s) 381, 591, 1109
HpyCH4III ACNGT 5 cut(s) 70, 161, 669, 900, 1033
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 7 cut(s) 8, 362, 422, 575, 830, 938, 1132
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 494, 527, 581
HpyF3I CTNAG 3 cut(s) 489, 857, 1068
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 6 cut(s) 40, 109, 410, 530, 796, 1020
Kpn2I TCCGGA 1 cut(s) 272
KpnI GGTACC 1 cut(s) 570
Ksp22I TGATCA 1 cut(s) 879
Kzo9I GATC 4 cut(s) 353, 879, 964, 1077
LmnI GCTCC 4 cut(s) 187, 380, 838, 983
Lsp1109I GCAGC 2 cut(s) 434, 927
LweI GCATC 5 cut(s) 148, 409, 594, 942, 965
MaeI CTAG 5 cut(s) 135, 350, 608, 989, 1142
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 4 cut(s) 50, 64, 70, 928
MalI GATC 4 cut(s) 355, 881, 966, 1079
MboI GATC 4 cut(s) 353, 879, 964, 1077
MboII GAAGA 6 cut(s) 18, 657, 660, 728, 878, 1118
MfeI CAATTG 2 cut(s) 758, 933
MflI RGATCY 2 cut(s) 353, 1077
MhlI GDGCHC 1 cut(s) 778
MlsI TGGCCA 1 cut(s) 405
MluCI AATT 8 cut(s) 244, 302, 688, 711, 758, 852, 933, 947
MluNI TGGCCA 1 cut(s) 405
MmeI TCCRAC 1 cut(s) 141
Mox20I TGGCCA 1 cut(s) 405
MroI TCCGGA 1 cut(s) 272
MroXI GAANNNNTTC 1 cut(s) 280
MscI TGGCCA 1 cut(s) 405
MseI TTAA 2 cut(s) 501, 824
Msp20I TGGCCA 1 cut(s) 405
MspCI CTTAAG 1 cut(s) 823
MspI CCGG 3 cut(s) 185, 273, 836
MspR9I CCNGG 1 cut(s) 378
MunI CAATTG 2 cut(s) 758, 933
MvaI CCWGG 1 cut(s) 378
MwoI GCNNNNNNNGC 4 cut(s) 179, 494, 527, 581
NcoI CCATGG 1 cut(s) 406
NdeII GATC 4 cut(s) 353, 879, 964, 1077
NlaIII CATG 6 cut(s) 40, 109, 410, 530, 796, 1020
NlaIV GGNNCC 4 cut(s) 183, 568, 802, 811
NmuCI GTSAC 1 cut(s) 928
NspI RCATGY 1 cut(s) 1020
PciI ACATGT 1 cut(s) 1016
PdmI GAANNNNTTC 1 cut(s) 280
PfeI GAWTC 2 cut(s) 467, 1123
PfoI TCCNGGA 1 cut(s) 376
PkrI GCNGC 3 cut(s) 7, 424, 917
PscI ACATGT 1 cut(s) 1016
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 4 cut(s) 183, 568, 802, 811
PspPI GGNCC 3 cut(s) 270, 293, 801
PstI CTGCAG 3 cut(s) 10, 364, 1134
PsuI RGATCY 2 cut(s) 353, 1077
RsaI GTAC 5 cut(s) 258, 415, 547, 568, 1140
RsaNI GTAC 5 cut(s) 257, 414, 546, 567, 1139
Rsr2I CGGWCCG 1 cut(s) 270
RsrII CGGWCCG 1 cut(s) 270
SaqAI TTAA 2 cut(s) 501, 824
SatI GCNGC 3 cut(s) 6, 423, 916
Sau3AI GATC 4 cut(s) 353, 879, 964, 1077
Sau96I GGNCC 3 cut(s) 270, 293, 801
ScrFI CCNGG 1 cut(s) 378
SduI GDGCHC 1 cut(s) 778
SfaNI GCATC 5 cut(s) 148, 409, 594, 942, 965
SfcI CTRYAG 3 cut(s) 6, 360, 1130
SinI GGWCC 3 cut(s) 270, 293, 801
SmlI CTYRAG 4 cut(s) 823, 842, 1004, 1073
SmoI CTYRAG 4 cut(s) 823, 842, 1004, 1073
Sse9I AATT 8 cut(s) 244, 302, 688, 711, 758, 852, 933, 947
SsiI CCGC 1 cut(s) 1064
SspI AATATT 2 cut(s) 337, 445
SspMI CTAG 5 cut(s) 135, 350, 608, 989, 1142
StyD4I CCNGG 1 cut(s) 376
StyI CCWWGG 2 cut(s) 134, 406
TaaI ACNGT 5 cut(s) 70, 161, 669, 900, 1033
TaiI ACGT 1 cut(s) 66
TaqI TCGA 2 cut(s) 204, 631
TaqII GACCGA 2 cut(s) 258, 310
TasI AATT 8 cut(s) 244, 302, 688, 711, 758, 852, 933, 947
TfiI GAWTC 2 cut(s) 467, 1123
Tru1I TTAA 2 cut(s) 501, 824
Tru9I TTAA 2 cut(s) 501, 824
TscAI CASTG 3 cut(s) 577, 771, 926
TseFI GTSAC 1 cut(s) 928
TseI GCWGC 3 cut(s) 5, 422, 915
Tsp45I GTSAC 1 cut(s) 928
TspDTI ATGAA 2 cut(s) 122, 809
TspRI CASTG 3 cut(s) 577, 771, 926
Vha464I CTTAAG 1 cut(s) 823
VpaK11BI GGWCC 3 cut(s) 270, 293, 801
XceI RCATGY 1 cut(s) 1020
XmaJI CCTAGG 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 280
XspI CTAG 5 cut(s) 135, 350, 608, 989, 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.