Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0000249

Physical Location & Seq

Genomic Coordinates Forward (+)

42494859 .. 42496070

Gene Structure 1212 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025146

Sequence Viewer

CDS Protein Promoter (2k)

Length: 999 bp

ATGTCTGCTTTAAATAGAGCAGAGTCTCTTCAGGGCAAGAGCAAACCAGAAAAGCCATTCCTTCATTCCCTTCTTCCCAGTTTGCAACATTTTCATCTTTTCTTTCTAGCCATGACTAGAAAGAAGGTGAAACTTACCTACATCACTAATGATAGTGCTCGAAAAGCAACATATAAGAAAAGGAAGAAGGGTCTCATGAAAAAGGTGAGTGAACTCACCACCCTTTGTGACATTGATGCTTGTGCTATTATCTACAGCCCTTACGACTCCCAGCCTGAAATTTGGCCTTCCCCCTTGGGAGTCCAACGTGTGCTTGCGCAGTTCAAGACCATGCCTGAAATGGAGCAAAGCAAGAAGATGGTGAATCAGGAGACTTTCCTGAGGCAAAGAATCAACAAGGCTCAGGAACAGCTTAAGAAGCAAAAGAAAGACAACCGGGAAAAGGAGATGGCTCGTGTGATGTTCCAAAGCCTAACTGGAAAGTCCCTCCAGGGCTTGACACTGTCAGACCTGACTGATCTCGGCTGGGTCATTGACCAACACCTGAAGGAGTTACATCTCAGGATGAAGAACATTAAGGAGGAGGCTGTGAAGAGCCAGAACCAAGTGCAAGGAGCACAAGGGGTGTCAGCTGCTGCAACGGATCATAGAATGGCAGCAATGAAGAGTAATGGAGAGGGCAGCAGCAGCAACATGGCAATGAAGACTGGTGAAGAGGGCAGCAATATGCAGCATGCAGCCCTTGACAGGCAAGTTTTTGATCACATGCATAACATGGAGGCAATGCAGCTCCAAATGATGCAGATGGCAGCTCAGAAGCAGCAGAACCCTTGGTTCATGGACATGATGAATGCGCCACCACCAACACCACTGCCGCATCCAGATCATCATCAGCATATGGGGTTTACCGGGGAGGATATGATGCTTCCTTTCGCGGATCATAACCACAGTACTATGTGGCCAAATCCTCCTCCAGCTGGCTGCTTTAATTTTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043076 GO:0043078 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SRF-TF

Protein Analysis

333

Amino Acids

37.85

Weight (kDa)

9.33

Isoelectric Point (pI)

48.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SRF-TF	PF00319	47 - 88	1.1e-17	SRF-type transcription factor (DNA-binding and dimerisation domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22590 AT1G22590 AT2G28700 AT3G05860 AT3G05860 AT3G05860 AT5G26630 AT5G27810 AT5G48670
fragaria_vesca	FvH4_6g08460 FvH4_6g08570 FvH4_6g21170 FvH4_6g43410 FvH4_7g09730
malus_domestica	MD04G1186100.v1.1 MD04G1186200.v1.1 MD04G1186300.v1.1 MD06G1013100.v1.1 MD06G1013200.v1.1 MD06G1013500.v1.1 MD06G1013600.v1.1 MD08G1200100.v1.1 MD12G1199500.v1.1 MD12G1199600.v1.1 MD12G1199700.v1.1 MD15G1387300.v1.1
prunus_persica	Prupe.1G533600_v2.0.a1 Prupe.1G533700_v2.0.a1 Prupe.3G020400_v2.0.a1 Prupe.6G303900_v2.0.a1 Prupe.6G304100_v2.0.a1 Prupe.7G092700_v2.0.a1
pyrus_communis	pycom04g16460 pycom06g01090 pycom12g18580 pycom12g18590
rosa_chinensis	RchiOBHm_Chr1g0321431 RchiOBHm_Chr1g0336291 RchiOBHm_Chr1g0346681 RchiOBHm_Chr1g0346741 RchiOBHm_Chr1g0347651 RchiOBHm_Chr3g0458611 RchiOBHm_Chr6g0267971 RchiOBHm_Chr6g0295031 RchiOBHm_Chr6g0296451 RchiOBHm_Chr7g0222461 RchiOBHm_Chr7g0222491 RchiOBHm_Chr7g0222511 RchiOBHm_Chr7g0223301
rosa_laevigata	RLG00000002052 RLG00000002057 RLG00000011691 RLG00000025146 RLG00000028786 RLG00000029375 RLG00000030422
rosa_multiflora	Rmu_co8262069.1_g000001 Rmu_sc0000087.1_g000025 Rmu_sc0000724.1_g000014 Rmu_sc0001453.1_g000004 Rmu_sc0001634.1_g000008 Rmu_sc0002096.1_g000035 Rmu_sc0002349.1_g000003 Rmu_sc0002349.1_g000024 Rmu_sc0003232.1_g000005 Rmu_sc0004404.1_g000010 Rmu_sc0004404.1_g000018 Rmu_sc0004638.1_g000024 Rmu_sc0005124.1_g000012 Rmu_sc0008767.1_g000014 Rmu_sc0014221.1_g000005 Rmu_sc0038216.1_g000002 Rmu_ssc0000146.1_g000019 Rmu_ssc0000146.1_g000023
rosa_roxburghii	Rroxscaffold_1G00004980 Rroxscaffold_3G00236720 Rroxscaffold_3G00236940 Rroxscaffold_4G00308370 Rroxscaffold_4G00308400 Rroxscaffold_4G00315810 Rroxscaffold_6G00420920 Rroxscaffold_7G00171520 Rroxscaffold_7G00200280
rosa_rugosa	Rorug01G0032700 Rorug01G0123500 Rorug01G0182200 Rorug01G0182400 Rorug01G0188300 Rorug01G0188500 Rorug03G0030200 Rorug06G0033600 Rorug06G0249000 Rorug06G0262500 Rorug07G0208800 Rorug07G0209700 Rorug07G0210100 Rorug07G0215900 Rorug07G0216000
rosa_samantha	Rh1AG044400 Rh1AG144000 Rh1AG201000 Rh1BG042600 Rh1BG113600 Rh1BG167200 Rh1BG171100 Rh1CG135700 Rh1DG150600 Rh3BG091400 Rh6AG157800 Rh6AG373900 Rh6BG156100 Rh6BG159900 Rh6BG369500 Rh6BG382500 Rh6DG141300 Rh6DG145600 Rh6DG362600 Rh6DG375000 Rh7AG351000 Rh7AG351600 Rh7AG351800 Rh7BG342000 Rh7BG342400 Rh7CG368300 Rh7CG369000 Rh7CG369200
rosa_wichuraiana	Rw0G015210 Rw0G021330 Rw1G003960 Rw1G016840 Rw3G007550 Rw6G013730 Rw6G032610 Rw7G029680 Rw7G030010

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	318
AccII	CGCG	1 cut(s)	935
AciI	CCGC	2 cut(s)	875, 935
AclWI	GGATC	2 cut(s)	651, 945
AcoI	YGGCCR	1 cut(s)	959
AcsI	RAATTY	1 cut(s)	279
AcuI	CTGAAG	2 cut(s)	14, 566
AfaI	GTAC	1 cut(s)	952
AfiI	CCNNNNNNNGG	3 cut(s)	442, 747, 977
AflII	CTTAAG	1 cut(s)	413
AflIII	ACRYGT	1 cut(s)	307
AgsI	TTSAA	1 cut(s)	325
AjnI	CCWGG	1 cut(s)	489
AluBI	AGCT	5 cut(s)	412, 632, 790, 812, 977
AluI	AGCT	5 cut(s)	412, 632, 790, 812, 977
Alw21I	GWGCWC	2 cut(s)	160, 619
Alw26I	GTCTC	3 cut(s)	30, 197, 365
AlwI	GGATC	2 cut(s)	651, 945
AlwNI	CAGNNNCTG	1 cut(s)	635
AoxI	GGCC	2 cut(s)	284, 959
ApoI	RAATTY	1 cut(s)	279
AspLEI	GCGC	2 cut(s)	319, 856
AsuC2I	CCSGG	2 cut(s)	437, 910
AsuHPI	GGTGA	5 cut(s)	139, 208, 217, 373, 722
AxyI	CCTNAGG	1 cut(s)	380
BalI	TGGCCA	1 cut(s)	961
BauI	CACGAG	1 cut(s)	453
BbsI	GAAGAC	1 cut(s)	710
Bbv12I	GWGCWC	2 cut(s)	160, 619
BccI	CCATC	3 cut(s)	352, 442, 799
BciT130I	CCWGG	1 cut(s)	491
BclI	TGATCA	1 cut(s)	760
BcnI	CCSGG	2 cut(s)	437, 910
BcoDI	GTCTC	3 cut(s)	30, 197, 365
BfaI	CTAG	2 cut(s)	107, 117
BfmI	CTRYAG	1 cut(s)	253
BfrI	CTTAAG	1 cut(s)	413
BmcAI	AGTACT	1 cut(s)	952
Bme1390I	CCNGG	3 cut(s)	437, 491, 910
BmrFI	CCNGG	3 cut(s)	437, 491, 910
BmrI	ACTGGG	1 cut(s)	72
BmsI	GCATC	4 cut(s)	226, 789, 886, 912
BmuI	ACTGGG	1 cut(s)	72
BpiI	GAAGAC	1 cut(s)	710
BpmI	CTGGAG	2 cut(s)	473, 957
Bpu10I	CCTNAGC	1 cut(s)	402
BpuMI	CCSGG	2 cut(s)	437, 910
BsaBI	GATNNNNATC	1 cut(s)	888
BsaI	GGTCTC	1 cut(s)	197
BsaJI	CCNNGG	4 cut(s)	294, 490, 830, 909
Bsc4I	CCNNNNNNNGG	3 cut(s)	442, 747, 977
Bse1I	ACTGG	3 cut(s)	78, 481, 712
Bse21I	CCTNAGG	1 cut(s)	380
Bse3DI	GCAATG	3 cut(s)	666, 705, 789
Bse8I	GATNNNNATC	1 cut(s)	888
BseBI	CCWGG	1 cut(s)	491
BseDI	CCNNGG	4 cut(s)	294, 490, 830, 909
BseGI	GGATG	2 cut(s)	570, 877
BseJI	GATNNNNATC	1 cut(s)	888
BseLI	CCNNNNNNNGG	3 cut(s)	442, 747, 977
BseMI	GCAATG	3 cut(s)	666, 705, 789
BseMII	CTCAG	4 cut(s)	371, 416, 574, 827
BseNI	ACTGG	3 cut(s)	78, 481, 712
BseRI	GAGGAG	2 cut(s)	596, 960
BseYI	CCCAGC	2 cut(s)	270, 525
Bsh1236I	CGCG	1 cut(s)	935
BshFI	GGCC	2 cut(s)	286, 961
BsiHKAI	GWGCWC	2 cut(s)	160, 619
BsiSI	CCGG	2 cut(s)	436, 909
BslFI	GGGAC	1 cut(s)	469
BslI	CCNNNNNNNGG	3 cut(s)	442, 747, 977
BsmAI	GTCTC	3 cut(s)	30, 197, 365
BsmFI	GGGAC	1 cut(s)	469
BsmI	GAATGC	1 cut(s)	856
BsnI	GGCC	2 cut(s)	286, 961
Bso31I	GGTCTC	1 cut(s)	197
Bsp1286I	GDGCHC	2 cut(s)	160, 619
Bsp143I	GATC	5 cut(s)	517, 643, 760, 883, 937
BspACI	CCGC	2 cut(s)	875, 935
BspANI	GGCC	2 cut(s)	286, 961
BspCNI	CTCAG	4 cut(s)	372, 415, 573, 826
BspFNI	CGCG	1 cut(s)	935
BspHI	TCATGA	1 cut(s)	195
BspPI	GGATC	2 cut(s)	651, 945
BspQI	GCTCTTC	1 cut(s)	587
BspTI	CTTAAG	1 cut(s)	413
BspTNI	GGTCTC	1 cut(s)	197
BsrDI	GCAATG	3 cut(s)	666, 705, 789
BsrI	ACTGG	3 cut(s)	78, 481, 712
BssECI	CCNNGG	4 cut(s)	294, 490, 830, 909
BssMI	GATC	5 cut(s)	517, 643, 760, 883, 937
BssSI	CACGAG	1 cut(s)	453
BssT1I	CCWWGG	2 cut(s)	294, 830
Bst2BI	CACGAG	1 cut(s)	453
Bst2UI	CCWGG	1 cut(s)	491
Bst4CI	ACNGT	2 cut(s)	504, 950
Bst6I	CTCTTC	4 cut(s)	33, 587, 659, 708
BstAFI	CTTAAG	1 cut(s)	413
BstC8I	GCNNGC	3 cut(s)	315, 735, 979
BstDEI	CTNAG	4 cut(s)	380, 402, 560, 813
BstF5I	GGATG	2 cut(s)	570, 877
BstFNI	CGCG	1 cut(s)	935
BstHHI	GCGC	2 cut(s)	319, 856
BstKTI	GATC	5 cut(s)	520, 646, 763, 886, 940
BstMAI	GTCTC	3 cut(s)	30, 197, 365
BstMBI	GATC	5 cut(s)	517, 643, 760, 883, 937
BstMWI	GCNNNNNNNGC	3 cut(s)	164, 418, 687
BstNI	CCWGG	1 cut(s)	491
BstNSI	RCATGY	2 cut(s)	737, 769
BstSCI	CCNGG	3 cut(s)	435, 489, 908
BstSFI	CTRYAG	1 cut(s)	253
BstUI	CGCG	1 cut(s)	935
BstV2I	GAAGAC	1 cut(s)	710
Bsu36I	CCTNAGG	1 cut(s)	380
BsuRI	GGCC	2 cut(s)	286, 961
BtsCI	GGATG	2 cut(s)	570, 877
BtsI	GCAGTG	1 cut(s)	869
BtsIMutI	CAGTG	2 cut(s)	500, 869
Cac8I	GCNNGC	3 cut(s)	315, 735, 979
CaiI	CAGNNNCTG	1 cut(s)	635
CciI	TCATGA	1 cut(s)	195
CfoI	GCGC	2 cut(s)	319, 856
Csp6I	GTAC	1 cut(s)	951
CviAII	CATG	9 cut(s)	112, 196, 331, 694, 734, 766, 775, 838, 844
CviQI	GTAC	1 cut(s)	951
DdeI	CTNAG	4 cut(s)	380, 402, 560, 813
DpnI	GATC	5 cut(s)	519, 645, 762, 885, 939
DpnII	GATC	5 cut(s)	517, 643, 760, 883, 937
DraI	TTTAAA	1 cut(s)	12
EaeI	YGGCCR	1 cut(s)	959
Eam1104I	CTCTTC	4 cut(s)	33, 587, 659, 708
EarI	CTCTTC	4 cut(s)	33, 587, 659, 708
Eco130I	CCWWGG	2 cut(s)	294, 830
Eco31I	GGTCTC	1 cut(s)	197
Eco57I	CTGAAG	2 cut(s)	14, 566
Eco81I	CCTNAGG	1 cut(s)	380
EcoRII	CCWGG	1 cut(s)	489
EcoT14I	CCWWGG	2 cut(s)	294, 830
EcoT22I	ATGCAT	1 cut(s)	771
ErhI	CCWWGG	2 cut(s)	294, 830
FaeI	CATG	9 cut(s)	115, 199, 334, 697, 737, 769, 778, 841, 847
FaqI	GGGAC	1 cut(s)	469
FatI	CATG	9 cut(s)	111, 195, 330, 693, 733, 765, 774, 837, 843
FauNDI	CATATG	1 cut(s)	897
FbaI	TGATCA	1 cut(s)	760
FokI	GGATG	2 cut(s)	577, 864
FspBI	CTAG	2 cut(s)	107, 117
FspI	TGCGCA	1 cut(s)	318
GlaI	GCGC	2 cut(s)	318, 855
GsaI	CCCAGC	2 cut(s)	274, 529
GsuI	CTGGAG	2 cut(s)	473, 957
HaeIII	GGCC	2 cut(s)	286, 961
HapII	CCGG	2 cut(s)	436, 909
HhaI	GCGC	2 cut(s)	319, 856
Hin1II	CATG	9 cut(s)	115, 199, 334, 697, 737, 769, 778, 841, 847
Hin6I	GCGC	2 cut(s)	317, 854
HinP1I	GCGC	2 cut(s)	317, 854
HinfI	GANTC	5 cut(s)	23, 266, 300, 364, 390
HpaII	CCGG	2 cut(s)	436, 909
HphI	GGTGA	5 cut(s)	139, 208, 217, 373, 722
Hpy166II	GTNNAC	2 cut(s)	212, 906
Hpy188I	TCNGA	2 cut(s)	508, 816
Hpy188III	TCNNGA	7 cut(s)	196, 325, 368, 379, 404, 562, 881
Hpy8I	GTNNAC	2 cut(s)	212, 906
HpyAV	CCTTC	6 cut(s)	71, 80, 118, 181, 297, 541
HpyCH4III	ACNGT	2 cut(s)	504, 950
HpyCH4IV	ACGT	1 cut(s)	307
HpyCH4V	TGCA	8 cut(s)	85, 610, 638, 730, 737, 769, 787, 802
HpyF10VI	GCNNNNNNNGC	3 cut(s)	164, 418, 687
HpyF3I	CTNAG	4 cut(s)	380, 402, 560, 813
HpySE526I	ACGT	1 cut(s)	307
Hsp92II	CATG	9 cut(s)	115, 199, 334, 697, 737, 769, 778, 841, 847
HspAI	GCGC	2 cut(s)	317, 854
Ksp22I	TGATCA	1 cut(s)	760
Kzo9I	GATC	5 cut(s)	517, 643, 760, 883, 937
LguI	GCTCTTC	1 cut(s)	587
LmnI	GCTCC	3 cut(s)	343, 614, 795
LweI	GCATC	4 cut(s)	226, 789, 886, 912
MaeI	CTAG	2 cut(s)	107, 117
MaeII	ACGT	1 cut(s)	307
MaeIII	GTNAC	2 cut(s)	227, 552
MalI	GATC	5 cut(s)	519, 645, 762, 885, 939
MboI	GATC	5 cut(s)	517, 643, 760, 883, 937
MboII	GAAGA	9 cut(s)	20, 65, 196, 367, 580, 604, 676, 715, 725
MhlI	GDGCHC	2 cut(s)	160, 619
MlsI	TGGCCA	1 cut(s)	961
MluCI	AATT	2 cut(s)	279, 988
MluNI	TGGCCA	1 cut(s)	961
MlyI	GAGTC	3 cut(s)	32, 260, 309
MmeI	TCCRAC	1 cut(s)	328
Mox20I	TGGCCA	1 cut(s)	961
Mph1103I	ATGCAT	1 cut(s)	771
MscI	TGGCCA	1 cut(s)	961
MseI	TTAA	4 cut(s)	11, 414, 576, 987
MslI	CAYNNNNRTG	2 cut(s)	698, 842
Msp20I	TGGCCA	1 cut(s)	961
MspA1I	CMGCKG	2 cut(s)	632, 977
MspCI	CTTAAG	1 cut(s)	413
MspI	CCGG	2 cut(s)	436, 909
MspR9I	CCNGG	3 cut(s)	437, 491, 910
Mva1269I	GAATGC	1 cut(s)	856
MvaI	CCWGG	1 cut(s)	491
MvnI	CGCG	1 cut(s)	935
MwoI	GCNNNNNNNGC	3 cut(s)	164, 418, 687
NciI	CCSGG	2 cut(s)	437, 910
NdeI	CATATG	1 cut(s)	897
NdeII	GATC	5 cut(s)	517, 643, 760, 883, 937
NlaIII	CATG	9 cut(s)	115, 199, 334, 697, 737, 769, 778, 841, 847
NmeAIII	GCCGAG	1 cut(s)	501
NmuCI	GTSAC	1 cut(s)	227
NsbI	TGCGCA	1 cut(s)	318
NsiI	ATGCAT	1 cut(s)	771
NspI	RCATGY	2 cut(s)	737, 769
PaeI	GCATGC	1 cut(s)	737
PagI	TCATGA	1 cut(s)	195
PciSI	GCTCTTC	1 cut(s)	587
PctI	GAATGC	1 cut(s)	856
PfeI	GAWTC	2 cut(s)	364, 390
PflFI	GACNNNGTC	1 cut(s)	502
PleI	GAGTC	3 cut(s)	31, 260, 308
PpsI	GAGTC	3 cut(s)	31, 260, 308
Psp6I	CCWGG	1 cut(s)	489
PspFI	CCCAGC	2 cut(s)	270, 525
PspGI	CCWGG	1 cut(s)	489
PstNI	CAGNNNCTG	1 cut(s)	635
PsyI	GACNNNGTC	1 cut(s)	502
PvuII	CAGCTG	2 cut(s)	632, 977
RsaI	GTAC	1 cut(s)	952
RsaNI	GTAC	1 cut(s)	951
RseI	CAYNNNNRTG	2 cut(s)	698, 842
SapI	GCTCTTC	1 cut(s)	587
SaqAI	TTAA	4 cut(s)	11, 414, 576, 987
Sau3AI	GATC	5 cut(s)	517, 643, 760, 883, 937
ScaI	AGTACT	1 cut(s)	952
SchI	GAGTC	3 cut(s)	32, 260, 309
ScrFI	CCNGG	3 cut(s)	437, 491, 910
SduI	GDGCHC	2 cut(s)	160, 619
SfaNI	GCATC	4 cut(s)	226, 789, 886, 912
SfcI	CTRYAG	1 cut(s)	253
SmiMI	CAYNNNNRTG	2 cut(s)	698, 842
SmlI	CTYRAG	1 cut(s)	413
SmoI	CTYRAG	1 cut(s)	413
SphI	GCATGC	1 cut(s)	737
Sse9I	AATT	2 cut(s)	279, 988
SsiI	CCGC	2 cut(s)	875, 935
SspMI	CTAG	2 cut(s)	107, 117
StyD4I	CCNGG	3 cut(s)	435, 489, 908
StyI	CCWWGG	2 cut(s)	294, 830
TaaI	ACNGT	2 cut(s)	504, 950
TaiI	ACGT	1 cut(s)	310
TaqI	TCGA	1 cut(s)	160
TasI	AATT	2 cut(s)	279, 988
TatI	WGTACW	1 cut(s)	950
TauI	GCSGC	1 cut(s)	877
TfiI	GAWTC	2 cut(s)	364, 390
Tru1I	TTAA	4 cut(s)	11, 414, 576, 987
Tru9I	TTAA	4 cut(s)	11, 414, 576, 987
TscAI	CASTG	2 cut(s)	507, 876
TseFI	GTSAC	1 cut(s)	227
Tsp45I	GTSAC	1 cut(s)	227
TspDTI	ATGAA	8 cut(s)	53, 83, 212, 581, 677, 716, 826, 863
TspGWI	ACGGA	1 cut(s)	656
TspRI	CASTG	2 cut(s)	507, 876
Tth111I	GACNNNGTC	1 cut(s)	502
Vha464I	CTTAAG	1 cut(s)	413
XapI	RAATTY	1 cut(s)	279
XceI	RCATGY	2 cut(s)	737, 769
XcmI	CCANNNNNNNNNTGG	2 cut(s)	337, 473
XspI	CTAG	2 cut(s)	107, 117
ZrmI	AGTACT	1 cut(s)	952
Zsp2I	ATGCAT	1 cut(s)	771

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.