FvH4_2g26690

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013852
Physical Location & Seq
Forward (+)
21383985 .. 21387442
3458 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26690.t2

Sequence Viewer

Length: 1149 bp
ATGTCGACTAGCATGTGGAAGAAGGCAAAACCGTATTTGCTGACTATTGGTTTGCAATTTGGAAGTGCAGGGATGTACATCATAAGCACTGTGACTCTCAACCATGGCATGAATCGATACGTTCTCATTGTGTATCGCAATGCTATTGCAGCTCTTGTTCTCCTTCCTTTTGCTTTGGTTCTTGAAAGGAAAACAAGGCCCAAAATGACGCTTTCAATCTTTTTTCACATAATGGTGCTGGGATTTCTAGAGCCGGTCCTTGATCAGGGCTTTGCTTACTTGGGAATGAATTATACTTCAGCATCATTCACTTCTGCCATCATGAATGCCGTTCCCTCTGTTACCTTTGTAATTTCCGTCATTTTCCGGATAGAGCAAGTAAACATTAAGGAGGTCCGGAGTCAAGCAAAGGTGATAGGAACCCTAGTAAGCTTTGGGGGAGCTTTGTTGATGGCAACGTACAAAGGCCCTGCCATTAACATAGTAGGCTCACATGATAACAGCAGCCACCATGAAAGTAGCAGCAGCAACACTTCGGGCTCACACTGGGCAATGGGGACGCTCTTCATCCTCATAGGCTGTGTGGCTTGGTCCTGTTTCTACGTGTTACAATCTGTAACAGTGAAGAAATACCCGGCAAACATATCTCTGTCCTGCTGGATATGCACCATGGGCGCCATGCAAGGTGCAGCAGTAGCTTTTGTTGCAGAGCGCCGTCCTAGTGCATGGGCTGTTGGCATGGATACAAGGCTCCTAGCACCTCTTTATACGGGAATAGTTAGTTCTGGAATTACATACTACGTGCAGGGATTGGTAATGAAAACGAGAGGACCAGTGTTTGTGACAGCTTTTAACCCTCTTTGCATGATCCTTGTCGCAATTCTGGCTTCGTTCATTCTAGCTGAGAAACTGCACCTGGGCAGTATCATTGGAGGGGTTATAATCGCCATCGGCCTTTATGCTGTGGTTTGGGGAAAAAGCAAGGACTACGGTACTAGCCCAAGCAGTGATCCCTCTGACCTCAAGGATGAGGATGAAGTTCACAAATTACCAGTTACTACAACCAACGGGACTAAAATGTCCATTATTGGTGACAAAGATAGCAGCAACAAGCCAACAGAGATGCAATATATGACGGTCCAAAAATAA

Protein Analysis

383

Amino Acids

41.46

Weight (kDa)

9.29

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 90 - 228 5.1e-13 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013852)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08290 AT4G08290
fragaria_vesca FvH4_2g26690 FvH4_2g26690
prunus_persica Prupe.1G500600_v2.0.a1
pyrus_communis pycom08g14200
rosa_chinensis RchiOBHm_Chr6g0295531
rosa_laevigata RLG00000011793
rosa_multiflora Rmu_sc0005000.1_g000013
rosa_roxburghii Rroxscaffold_7G00172390
rosa_rugosa Rorug06G0251700
rosa_samantha Rh2AG083600 Rh6AG364900 Rh6BG373300 Rh6CG379500 Rh6DG366000
rosa_wichuraiana Rw6G031870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 941
AasI GACNNNNNNGTC 1 cut(s) 1078
AccB1I GGYRCC 1 cut(s) 674
AccI GTMKAC 1 cut(s) 5
AccIII TCCGGA 2 cut(s) 366, 396
AclWI GGATC 2 cut(s) 862, 1004
AcuI CTGAAG 1 cut(s) 282
AcyI GRCGYC 1 cut(s) 675
AfaI GTAC 3 cut(s) 77, 461, 994
AfiI CCNNNNNNNGG 1 cut(s) 265
AflIII ACRYGT 1 cut(s) 603
AgsI TTSAA 2 cut(s) 185, 216
AjnI CCWGG 1 cut(s) 915
AluBI AGCT 6 cut(s) 152, 432, 443, 698, 848, 902
AluI AGCT 6 cut(s) 152, 432, 443, 698, 848, 902
AlwI GGATC 2 cut(s) 862, 1004
Aor13HI TCCGGA 2 cut(s) 366, 396
AoxI GGCC 3 cut(s) 197, 466, 952
ApeKI GCWGC 6 cut(s) 149, 504, 522, 525, 689, 1104
ArsI GACNNNNNNTTYG 2 cut(s) 34, 66
AspLEI GCGC 2 cut(s) 677, 714
AspS9I GGNCC 7 cut(s) 198, 256, 394, 467, 591, 830, 1138
AsuC2I CCSGG 1 cut(s) 635
AsuHPI GGTGA 2 cut(s) 424, 1103
AvaII GGWCC 5 cut(s) 256, 394, 591, 830, 1138
BanI GGYRCC 1 cut(s) 674
BanII GRGCYC 1 cut(s) 542
BbvI GCAGC 6 cut(s) 161, 516, 534, 537, 701, 1116
BccI CCATC 3 cut(s) 326, 445, 956
BceAI ACGGC 2 cut(s) 314, 699
BciT130I CCWGG 1 cut(s) 917
BciVI GTATCC 1 cut(s) 736
BclI TGATCA 1 cut(s) 262
BcnI CCSGG 1 cut(s) 635
BfaI CTAG 7 cut(s) 9, 248, 425, 720, 755, 899, 996
BfoI RGCGCY 2 cut(s) 678, 715
BfuI GTATCC 1 cut(s) 736
BisI GCNGC 6 cut(s) 150, 505, 523, 526, 690, 1105
BlsI GCNGC 6 cut(s) 151, 506, 524, 527, 691, 1106
Bme1390I CCNGG 2 cut(s) 635, 917
Bme18I GGWCC 5 cut(s) 256, 394, 591, 830, 1138
BmgT120I GGNCC 7 cut(s) 198, 256, 394, 467, 591, 830, 1138
BmiI GGNNCC 3 cut(s) 421, 676, 752
BmrFI CCNGG 2 cut(s) 635, 917
BmrI ACTGGG 1 cut(s) 556
BmsI GCATC 2 cut(s) 311, 1113
BmuI ACTGGG 1 cut(s) 556
BpuEI CTTGAG 1 cut(s) 1007
BpuMI CCSGG 1 cut(s) 635
Bsa29I ATCGAT 1 cut(s) 115
BsaAI YACGTR 2 cut(s) 604, 802
BsaBI GATNNNNATC 1 cut(s) 77
BsaHI GRCGYC 1 cut(s) 675
BsaJI CCNNGG 3 cut(s) 103, 669, 916
BsaWI WCCGGW 2 cut(s) 366, 396
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse118I RCCGGY 1 cut(s) 253
Bse1I ACTGG 3 cut(s) 551, 833, 1052
Bse3DI GCAATG 2 cut(s) 145, 558
Bse8I GATNNNNATC 1 cut(s) 77
BseAI TCCGGA 2 cut(s) 366, 396
BseBI CCWGG 1 cut(s) 917
BseCI ATCGAT 1 cut(s) 115
BseDI CCNNGG 3 cut(s) 103, 669, 916
BseGI GGATG 4 cut(s) 78, 567, 1033, 1039
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMI GCAATG 2 cut(s) 145, 558
BseMII CTCAG 1 cut(s) 894
BseNI ACTGG 3 cut(s) 551, 833, 1052
BseXI GCAGC 6 cut(s) 161, 516, 534, 537, 701, 1116
BseYI CCCAGC 1 cut(s) 238
BsgI GTGCAG 4 cut(s) 87, 708, 824, 896
BshFI GGCC 3 cut(s) 199, 468, 954
BshNI GGYRCC 1 cut(s) 674
BshVI ATCGAT 1 cut(s) 115
BsiSI CCGG 4 cut(s) 254, 367, 397, 635
BslFI GGGAC 2 cut(s) 571, 1084
BslI CCNNNNNNNGG 1 cut(s) 265
BsmFI GGGAC 2 cut(s) 571, 1084
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 3 cut(s) 199, 468, 954
Bsp1286I GDGCHC 1 cut(s) 542
Bsp13I TCCGGA 2 cut(s) 366, 396
Bsp1407I TGTACA 1 cut(s) 75
Bsp143I GATC 3 cut(s) 262, 867, 1009
Bsp19I CCATGG 2 cut(s) 103, 669
BspANI GGCC 3 cut(s) 199, 468, 954
BspCNI CTCAG 1 cut(s) 895
BspDI ATCGAT 1 cut(s) 115
BspEI TCCGGA 2 cut(s) 366, 396
BspHI TCATGA 1 cut(s) 321
BspLI GGNNCC 3 cut(s) 421, 676, 752
BspPI GGATC 2 cut(s) 862, 1004
BspQI GCTCTTC 1 cut(s) 569
BspT107I GGYRCC 1 cut(s) 674
BsrDI GCAATG 2 cut(s) 145, 558
BsrFI RCCGGY 1 cut(s) 253
BsrGI TGTACA 1 cut(s) 75
BsrI ACTGG 3 cut(s) 551, 833, 1052
BssAI RCCGGY 1 cut(s) 253
BssECI CCNNGG 3 cut(s) 103, 669, 916
BssMI GATC 3 cut(s) 262, 867, 1009
BssNI GRCGYC 1 cut(s) 675
BssT1I CCWWGG 2 cut(s) 103, 669
Bst2UI CCWGG 1 cut(s) 917
Bst4CI ACNGT 5 cut(s) 33, 91, 622, 992, 1138
Bst6I CTCTTC 1 cut(s) 569
BstACI GRCGYC 1 cut(s) 675
BstAUI TGTACA 1 cut(s) 75
BstBAI YACGTR 2 cut(s) 604, 802
BstDEI CTNAG 1 cut(s) 903
BstDSI CCRYGG 2 cut(s) 103, 669
BstENI CCTNNNNNAGG 1 cut(s) 263
BstF5I GGATG 4 cut(s) 78, 567, 1033, 1039
BstH2I RGCGCY 2 cut(s) 678, 715
BstHHI GCGC 2 cut(s) 677, 714
BstKTI GATC 3 cut(s) 265, 870, 1012
BstMBI GATC 3 cut(s) 262, 867, 1009
BstMWI GCNNNNNNNGC 6 cut(s) 149, 663, 672, 695, 704, 884
BstNI CCWGG 1 cut(s) 917
BstNSI RCATGY 1 cut(s) 16
BstSCI CCNGG 2 cut(s) 633, 915
BstV1I GCAGC 6 cut(s) 161, 516, 534, 537, 701, 1116
Bsu15I ATCGAT 1 cut(s) 115
BsuI GTATCC 1 cut(s) 736
BsuRI GGCC 3 cut(s) 199, 468, 954
BsuTUI ATCGAT 1 cut(s) 115
BtgI CCRYGG 2 cut(s) 103, 669
BtsCI GGATG 4 cut(s) 78, 567, 1033, 1039
BtsI GCAGTG 1 cut(s) 1012
BtsIMutI CAGTG 5 cut(s) 87, 544, 627, 840, 1012
CciI TCATGA 1 cut(s) 321
CfoI GCGC 2 cut(s) 677, 714
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 7 cut(s) 198, 256, 394, 467, 591, 830, 1138
ClaI ATCGAT 1 cut(s) 115
CseI GACGC 2 cut(s) 217, 568
Csp6I GTAC 3 cut(s) 76, 460, 993
CviQI GTAC 3 cut(s) 76, 460, 993
DdeI CTNAG 1 cut(s) 903
DinI GGCGCC 1 cut(s) 676
DpnI GATC 3 cut(s) 264, 869, 1011
DpnII GATC 3 cut(s) 262, 867, 1009
DrdI GACNNNNNNGTC 1 cut(s) 1078
DseDI GACNNNNNNGTC 1 cut(s) 1078
Eam1104I CTCTTC 1 cut(s) 569
EarI CTCTTC 1 cut(s) 569
Eco130I CCWWGG 2 cut(s) 103, 669
Eco24I GRGCYC 1 cut(s) 542
Eco47I GGWCC 5 cut(s) 256, 394, 591, 830, 1138
Eco57I CTGAAG 1 cut(s) 282
EcoNI CCTNNNNNAGG 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 915
EcoT14I CCWWGG 2 cut(s) 103, 669
EcoT38I GRGCYC 1 cut(s) 542
EgeI GGCGCC 1 cut(s) 676
EheI GGCGCC 1 cut(s) 676
ErhI CCWWGG 2 cut(s) 103, 669
FaqI GGGAC 2 cut(s) 571, 1084
FbaI TGATCA 1 cut(s) 262
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 6 cut(s) 150, 505, 523, 526, 690, 1105
FokI GGATG 4 cut(s) 85, 554, 1040, 1046
FriOI GRGCYC 1 cut(s) 542
Fsp4HI GCNGC 6 cut(s) 150, 505, 523, 526, 690, 1105
FspBI CTAG 7 cut(s) 9, 248, 425, 720, 755, 899, 996
GlaI GCGC 2 cut(s) 676, 713
GluI GCNGC 6 cut(s) 150, 505, 523, 526, 690, 1105
GsaI CCCAGC 1 cut(s) 242
HaeII RGCGCY 2 cut(s) 678, 715
HaeIII GGCC 3 cut(s) 199, 468, 954
HapII CCGG 4 cut(s) 254, 367, 397, 635
HgaI GACGC 2 cut(s) 217, 568
HhaI GCGC 2 cut(s) 677, 714
Hin1I GRCGYC 1 cut(s) 675
Hin6I GCGC 2 cut(s) 675, 712
HinP1I GCGC 2 cut(s) 675, 712
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 430
HinfI GANTC 3 cut(s) 94, 112, 400
HpaII CCGG 4 cut(s) 254, 367, 397, 635
HphI GGTGA 2 cut(s) 424, 1103
Hpy166II GTNNAC 3 cut(s) 6, 382, 1042
Hpy188I TCNGA 1 cut(s) 1018
Hpy188III TCNNGA 6 cut(s) 182, 248, 322, 367, 397, 786
Hpy8I GTNNAC 3 cut(s) 6, 382, 1042
HpyAV CCTTC 2 cut(s) 16, 173
HpyCH4III ACNGT 5 cut(s) 33, 91, 622, 992, 1138
HpyCH4IV ACGT 4 cut(s) 120, 458, 603, 801
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 663, 672, 695, 704, 884
HpyF3I CTNAG 1 cut(s) 903
HpySE526I ACGT 4 cut(s) 120, 458, 603, 801
Hsp92I GRCGYC 1 cut(s) 675
HspAI GCGC 2 cut(s) 675, 712
KasI GGCGCC 1 cut(s) 674
Kpn2I TCCGGA 2 cut(s) 366, 396
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 3 cut(s) 262, 867, 1009
LguI GCTCTTC 1 cut(s) 569
LmnI GCTCC 2 cut(s) 440, 756
Lsp1109I GCAGC 6 cut(s) 161, 516, 534, 537, 701, 1116
LweI GCATC 2 cut(s) 311, 1113
MaeI CTAG 7 cut(s) 9, 248, 425, 720, 755, 899, 996
MaeII ACGT 4 cut(s) 120, 458, 603, 801
MaeIII GTNAC 7 cut(s) 91, 340, 606, 616, 841, 1054, 1091
MalI GATC 3 cut(s) 264, 869, 1011
MboI GATC 3 cut(s) 262, 867, 1009
MboII GAAGA 3 cut(s) 31, 556, 637
MhlI GDGCHC 1 cut(s) 542
MluCI AATT 6 cut(s) 56, 289, 351, 789, 879, 1046
Mly113I GGCGCC 1 cut(s) 675
MlyI GAGTC 2 cut(s) 88, 409
MroI TCCGGA 2 cut(s) 366, 396
MseI TTAA 3 cut(s) 387, 477, 852
MslI CAYNNNNRTG 1 cut(s) 233
MspI CCGG 4 cut(s) 254, 367, 397, 635
MspR9I CCNGG 2 cut(s) 635, 917
Mva1269I GAATGC 1 cut(s) 331
MvaI CCWGG 1 cut(s) 917
MwoI GCNNNNNNNGC 6 cut(s) 149, 663, 672, 695, 704, 884
NarI GGCGCC 1 cut(s) 675
NciI CCSGG 1 cut(s) 635
NcoI CCATGG 2 cut(s) 103, 669
NdeII GATC 3 cut(s) 262, 867, 1009
NlaIV GGNNCC 3 cut(s) 421, 676, 752
NmuCI GTSAC 3 cut(s) 91, 841, 1091
NspI RCATGY 1 cut(s) 16
PagI TCATGA 1 cut(s) 321
PciSI GCTCTTC 1 cut(s) 569
PctI GAATGC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 112
PkrI GCNGC 6 cut(s) 151, 506, 524, 527, 691, 1106
PleI GAGTC 2 cut(s) 88, 408
PluTI GGCGCC 1 cut(s) 678
PpsI GAGTC 2 cut(s) 88, 408
Ppu21I YACGTR 2 cut(s) 604, 802
PsiI TTATAA 1 cut(s) 941
Psp6I CCWGG 1 cut(s) 915
PspFI CCCAGC 1 cut(s) 238
PspGI CCWGG 1 cut(s) 915
PspN4I GGNNCC 3 cut(s) 421, 676, 752
PspPI GGNCC 7 cut(s) 198, 256, 394, 467, 591, 830, 1138
RsaI GTAC 3 cut(s) 77, 461, 994
RsaNI GTAC 3 cut(s) 76, 460, 993
RseI CAYNNNNRTG 1 cut(s) 233
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 569
SaqAI TTAA 3 cut(s) 387, 477, 852
SatI GCNGC 6 cut(s) 150, 505, 523, 526, 690, 1105
Sau3AI GATC 3 cut(s) 262, 867, 1009
Sau96I GGNCC 7 cut(s) 198, 256, 394, 467, 591, 830, 1138
SchI GAGTC 2 cut(s) 88, 409
ScrFI CCNGG 2 cut(s) 635, 917
SduI GDGCHC 1 cut(s) 542
SfaNI GCATC 2 cut(s) 311, 1113
SfoI GGCGCC 1 cut(s) 676
SinI GGWCC 5 cut(s) 256, 394, 591, 830, 1138
SmiMI CAYNNNNRTG 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 1022
SmoI CTYRAG 1 cut(s) 1022
Sse9I AATT 6 cut(s) 56, 289, 351, 789, 879, 1046
SspDI GGCGCC 1 cut(s) 674
SspMI CTAG 7 cut(s) 9, 248, 425, 720, 755, 899, 996
StyD4I CCNGG 2 cut(s) 633, 915
StyI CCWWGG 2 cut(s) 103, 669
TaaI ACNGT 5 cut(s) 33, 91, 622, 992, 1138
TaiI ACGT 4 cut(s) 123, 461, 606, 804
TaqI TCGA 2 cut(s) 5, 115
TasI AATT 6 cut(s) 56, 289, 351, 789, 879, 1046
TatI WGTACW 1 cut(s) 75
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 3 cut(s) 387, 477, 852
Tru9I TTAA 3 cut(s) 387, 477, 852
TscAI CASTG 5 cut(s) 94, 551, 627, 840, 1012
TseFI GTSAC 3 cut(s) 91, 841, 1091
TseI GCWGC 6 cut(s) 149, 504, 522, 525, 689, 1104
Tsp45I GTSAC 3 cut(s) 91, 841, 1091
TspDTI ATGAA 8 cut(s) 125, 302, 338, 528, 556, 833, 883, 1050
TspGWI ACGGA 1 cut(s) 346
TspRI CASTG 5 cut(s) 94, 551, 627, 840, 1012
VpaK11BI GGWCC 5 cut(s) 256, 394, 591, 830, 1138
XagI CCTNNNNNAGG 1 cut(s) 263
XbaI TCTAGA 1 cut(s) 247
XceI RCATGY 1 cut(s) 16
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 7 cut(s) 9, 248, 425, 720, 755, 899, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.