Rorug06G0251700

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013852
Physical Location & Seq
Reverse (-)
39725445 .. 39726416
972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0251700.1

Sequence Viewer

Length: 972 bp
ATGGCAGCAACCCAAATCCCAGTGGTGGTTCTTGAATCCTCCGATGGCCGCAGGACCATGCCTGTGCTTGGCTTCGGCACAGCATCCGACAATTCACAACCAGAAGTTTTGATAGAAGCTGTTCTCGAGGCCATCAAGCTCGGTTACCGCCACTTCGACACGGCTTCCCTGTACGGCTCGGAGCAGAGTCTGGGAGTAGCCATAGCCCAAGCACTCAAACTCGGCCTCGTGGCTTCTCGAGACGAGCTCTTCATTACTTCCAAGCTTTGGCCTAATGATGCTCACCCTGATTTGGTTCTTCCTGCTCTAAACAAATCCCTTGAGAATCTTCAATTGGATTACCTTGATCTGTATCTGATCCACTGGCCGATCAGTGCCACGCCTGGGAAACTGAGTCACTCTCTCGAGGAGAAGGACCAAATGCCGATGGACTTCAAGGGTGTGTGGGCAGCCATGGAAGAAGCTCAGAGACGTGGCCTCACCAAATCCATTGGAATCAGTAATTTCTCTACCAAAAAGACTCAGAATTTGCTCTCTTTTGCTACCATTCCTCCTTCAGTCAATCAAGTTGAGATGAGCCCATTTTGGCAACAAAAGAAGCTCAGAGAATTTTGCAAAGCCAATGGTGTAGTTGTGACTGCCTTCTCCCCATTGGGTGCCATAGGAACCAGTTGGGGTACCAATCATGTTCTCGAAAGCAAAGTGCTTAATGAAATCGCTGAGGCTCGGGGAAAGACTGTTGCTCAGGTCTGCATTAGATGGGTTTATCAGGTAGGAGCAACACTTGCAGTCAAGAGCTACAACAAGGAGAGGTTGAAGCAGAATATGCAGGTGTTCGACTGGGAACTAACACAAGAGGATCTTGACAAGATCAGTCAAATCCCACAGCGCAAAATGATGCTTCGAGAAGAGATGGTGACGGCTACTGGCCCATACAAGTCCCTTGATGAGTTATGGGACGGAGAGTATTAA

Protein Analysis

323

Amino Acids

36.07

Weight (kDa)

5.67

Isoelectric Point (pI)

34.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 294 4.4e-57 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013852)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08290 AT4G08290
fragaria_vesca FvH4_2g26690 FvH4_2g26690
prunus_persica Prupe.1G500600_v2.0.a1
pyrus_communis pycom08g14200
rosa_chinensis RchiOBHm_Chr6g0295531
rosa_laevigata RLG00000011793
rosa_multiflora Rmu_sc0005000.1_g000013
rosa_roxburghii Rroxscaffold_7G00172390
rosa_rugosa Rorug06G0251700
rosa_samantha Rh2AG083600 Rh6AG364900 Rh6BG373300 Rh6CG379500 Rh6DG366000
rosa_wichuraiana Rw6G031870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 820
Acc36I ACCTGC 1 cut(s) 820
Acc65I GGTACC 1 cut(s) 677
AccB1I GGYRCC 2 cut(s) 656, 677
AccB7I CCANNNNNTGG 1 cut(s) 267
AciI CCGC 2 cut(s) 49, 148
AclWI GGATC 2 cut(s) 352, 867
AcoI YGGCCR 2 cut(s) 46, 365
AcsI RAATTY 2 cut(s) 526, 608
AcuI CTGAAG 1 cut(s) 540
AfaI GTAC 2 cut(s) 173, 679
AfiI CCNNNNNNNGG 5 cut(s) 25, 68, 267, 292, 384
AgsI TTSAA 4 cut(s) 35, 332, 436, 817
AjiI CACGTC 1 cut(s) 473
AjnI CCWGG 1 cut(s) 382
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 7 cut(s) 119, 139, 247, 265, 464, 601, 798
AluI AGCT 7 cut(s) 119, 139, 247, 265, 464, 601, 798
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 2 cut(s) 234, 463
AlwI GGATC 2 cut(s) 352, 867
AlwNI CAGNNNCTG 1 cut(s) 190
Ama87I CYCGRG 4 cut(s) 125, 237, 404, 726
AoxI GGCC 7 cut(s) 46, 129, 223, 269, 365, 475, 928
ApeKI GCWGC 2 cut(s) 5, 449
ApoI RAATTY 2 cut(s) 526, 608
ArsI GACNNNNNNTTYG 2 cut(s) 511, 543
Asp700I GAANNNNTTC 1 cut(s) 120
Asp718I GGTACC 1 cut(s) 677
AspLEI GCGC 1 cut(s) 891
AspS9I GGNCC 3 cut(s) 54, 415, 929
AsuHPI GGTGA 3 cut(s) 275, 472, 928
AvaI CYCGRG 4 cut(s) 125, 237, 404, 726
AvaII GGWCC 2 cut(s) 54, 415
BanI GGYRCC 2 cut(s) 656, 677
BanII GRGCYC 2 cut(s) 249, 581
BauI CACGAG 1 cut(s) 227
Bbv12I GWGCWC 1 cut(s) 249
BbvCI CCTCAGC 1 cut(s) 720
BbvI GCAGC 2 cut(s) 17, 461
BccI CCATC 5 cut(s) 38, 140, 421, 753, 907
BceAI ACGGC 3 cut(s) 177, 190, 936
BciT130I CCWGG 1 cut(s) 384
BcoDI GTCTC 2 cut(s) 234, 463
BfuAI ACCTGC 1 cut(s) 820
BisI GCNGC 3 cut(s) 6, 49, 450
BlsI GCNGC 3 cut(s) 7, 50, 451
Bme1390I CCNGG 1 cut(s) 384
Bme18I GGWCC 2 cut(s) 54, 415
BmeT110I CYCGRG 4 cut(s) 125, 237, 404, 726
BmgBI CACGTC 1 cut(s) 473
BmgT120I GGNCC 3 cut(s) 54, 415, 929
BmiI GGNNCC 3 cut(s) 658, 667, 679
BmrFI CCNGG 1 cut(s) 384
BmrI ACTGGG 2 cut(s) 14, 850
BmsI GCATC 3 cut(s) 92, 268, 888
BmuI ACTGGG 2 cut(s) 14, 850
BplI GAGNNNNNCTC 4 cut(s) 231, 263, 385, 417
Bpu10I CCTNAGC 2 cut(s) 720, 744
BpuEI CTTGAG 1 cut(s) 341
BsaBI GATNNNNATC 1 cut(s) 351
BsaJI CCNNGG 2 cut(s) 383, 453
BsaXI ACNNNNNCTCC 2 cut(s) 535, 565
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 68, 267, 292, 384
Bse1I ACTGG 5 cut(s) 20, 368, 669, 845, 931
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 1 cut(s) 384
BseDI CCNNGG 2 cut(s) 383, 453
BseGI GGATG 1 cut(s) 83
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 5 cut(s) 25, 68, 267, 292, 384
BseMII CTCAG 6 cut(s) 383, 479, 536, 616, 711, 758
BseNI ACTGG 5 cut(s) 20, 368, 669, 845, 931
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 2 cut(s) 17, 461
BshFI GGCC 7 cut(s) 48, 131, 225, 271, 367, 477, 930
BshNI GGYRCC 2 cut(s) 656, 677
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 4 cut(s) 125, 237, 404, 726
BslFI GGGAC 1 cut(s) 925
BslI CCNNNNNNNGG 5 cut(s) 25, 68, 267, 292, 384
BsmAI GTCTC 2 cut(s) 234, 463
BsmBI CGTCTC 2 cut(s) 234, 463
BsmFI GGGAC 1 cut(s) 925
BsnI GGCC 7 cut(s) 48, 131, 225, 271, 367, 477, 930
BsoBI CYCGRG 4 cut(s) 125, 237, 404, 726
Bsp1286I GDGCHC 2 cut(s) 249, 581
Bsp143I GATC 5 cut(s) 346, 357, 369, 859, 870
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 2 cut(s) 49, 148
BspANI GGCC 7 cut(s) 48, 131, 225, 271, 367, 477, 930
BspCNI CTCAG 6 cut(s) 384, 478, 535, 615, 712, 757
BspLI GGNNCC 3 cut(s) 658, 667, 679
BspMI ACCTGC 1 cut(s) 820
BspPI GGATC 2 cut(s) 352, 867
BspQI GCTCTTC 1 cut(s) 254
BspT107I GGYRCC 2 cut(s) 656, 677
BsrI ACTGG 5 cut(s) 20, 368, 669, 845, 931
BssECI CCNNGG 2 cut(s) 383, 453
BssMI GATC 5 cut(s) 346, 357, 369, 859, 870
BssSI CACGAG 1 cut(s) 227
BssT1I CCWWGG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 227
Bst2UI CCWGG 1 cut(s) 384
Bst4CI ACNGT 1 cut(s) 739
Bst6I CTCTTC 2 cut(s) 254, 903
BstAPI GCANNNNNTGC 2 cut(s) 785, 826
BstDEI CTNAG 6 cut(s) 392, 465, 522, 602, 720, 744
BstDSI CCRYGG 1 cut(s) 453
BstEII GGTNACC 1 cut(s) 143
BstF5I GGATG 1 cut(s) 83
BstHHI GCGC 1 cut(s) 891
BstKTI GATC 5 cut(s) 349, 360, 372, 862, 873
BstMAI GTCTC 2 cut(s) 234, 463
BstMBI GATC 5 cut(s) 346, 357, 369, 859, 870
BstMWI GCNNNNNNNGC 2 cut(s) 785, 826
BstNI CCWGG 1 cut(s) 384
BstPI GGTNACC 1 cut(s) 143
BstSCI CCNGG 1 cut(s) 382
BstV1I GCAGC 2 cut(s) 17, 461
BstX2I RGATCY 1 cut(s) 859
BstYI RGATCY 1 cut(s) 859
BsuRI GGCC 7 cut(s) 48, 131, 225, 271, 367, 477, 930
BtgI CCRYGG 1 cut(s) 453
BtrI CACGTC 1 cut(s) 473
BtsCI GGATG 1 cut(s) 83
BtsIMutI CAGTG 3 cut(s) 27, 361, 379
BveI ACCTGC 1 cut(s) 820
CaiI CAGNNNCTG 1 cut(s) 190
CfoI GCGC 1 cut(s) 891
Cfr13I GGNCC 3 cut(s) 54, 415, 929
Csp6I GTAC 2 cut(s) 172, 678
CviAII CATG 3 cut(s) 58, 454, 686
CviQI GTAC 2 cut(s) 172, 678
DdeI CTNAG 6 cut(s) 392, 465, 522, 602, 720, 744
DpnI GATC 5 cut(s) 348, 359, 371, 861, 872
DpnII GATC 5 cut(s) 346, 357, 369, 859, 870
EaeI YGGCCR 2 cut(s) 46, 365
Eam1104I CTCTTC 2 cut(s) 254, 903
EarI CTCTTC 2 cut(s) 254, 903
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 2 cut(s) 249, 581
Eco47I GGWCC 2 cut(s) 54, 415
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 540
Eco88I CYCGRG 4 cut(s) 125, 237, 404, 726
Eco91I GGTNACC 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 143
EcoRII CCWGG 1 cut(s) 382
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 2 cut(s) 249, 581
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 2 cut(s) 234, 463
FaeI CATG 3 cut(s) 61, 457, 689
FaiI YATR 8 cut(s) 59, 203, 455, 662, 687, 827, 934, 955
FalI AAGNNNNNCTT 2 cut(s) 846, 878
FaqI GGGAC 1 cut(s) 925
FatI CATG 3 cut(s) 57, 453, 685
Fnu4HI GCNGC 3 cut(s) 6, 49, 450
FokI GGATG 1 cut(s) 70
FriOI GRGCYC 2 cut(s) 249, 581
Fsp4HI GCNGC 3 cut(s) 6, 49, 450
GlaI GCGC 1 cut(s) 890
GluI GCNGC 3 cut(s) 6, 49, 450
HaeIII GGCC 7 cut(s) 48, 131, 225, 271, 367, 477, 930
HhaI GCGC 1 cut(s) 891
Hin1II CATG 3 cut(s) 61, 457, 689
Hin6I GCGC 1 cut(s) 889
HinP1I GCGC 1 cut(s) 889
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 6 cut(s) 35, 187, 325, 394, 495, 520
HphI GGTGA 3 cut(s) 275, 472, 928
Hpy188I TCNGA 7 cut(s) 43, 88, 181, 357, 468, 525, 605
Hpy188III TCNNGA 9 cut(s) 32, 125, 237, 239, 404, 692, 793, 863, 905
HpyAV CCTTC 3 cut(s) 406, 564, 652
HpyCH4III ACNGT 1 cut(s) 739
HpyCH4IV ACGT 1 cut(s) 472
HpyCH4V TGCA 4 cut(s) 615, 753, 788, 829
HpyF10VI GCNNNNNNNGC 2 cut(s) 785, 826
HpyF3I CTNAG 6 cut(s) 392, 465, 522, 602, 720, 744
HpySE526I ACGT 1 cut(s) 472
Hsp92II CATG 3 cut(s) 61, 457, 689
HspAI GCGC 1 cut(s) 889
KpnI GGTACC 1 cut(s) 681
Kzo9I GATC 5 cut(s) 346, 357, 369, 859, 870
LguI GCTCTTC 1 cut(s) 254
LmnI GCTCC 2 cut(s) 181, 776
Lsp1109I GCAGC 2 cut(s) 17, 461
LweI GCATC 3 cut(s) 92, 268, 888
MaeII ACGT 1 cut(s) 472
MaeIII GTNAC 4 cut(s) 143, 395, 634, 916
MalI GATC 5 cut(s) 348, 359, 371, 861, 872
MboI GATC 5 cut(s) 346, 357, 369, 859, 870
MboII GAAGA 5 cut(s) 241, 290, 320, 470, 920
MfeI CAATTG 1 cut(s) 332
MflI RGATCY 1 cut(s) 859
MhlI GDGCHC 2 cut(s) 249, 581
MluCI AATT 5 cut(s) 91, 332, 502, 526, 608
MlyI GAGTC 3 cut(s) 196, 403, 514
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 9 cut(s) 49, 121, 236, 400, 488, 561, 715, 804, 850
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 2 cut(s) 708, 970
MslI CAYNNNNRTG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 384
MunI CAATTG 1 cut(s) 332
MvaI CCWGG 1 cut(s) 384
MwoI GCNNNNNNNGC 2 cut(s) 785, 826
NcoI CCATGG 1 cut(s) 453
NdeII GATC 5 cut(s) 346, 357, 369, 859, 870
NlaIII CATG 3 cut(s) 61, 457, 689
NlaIV GGNNCC 3 cut(s) 658, 667, 679
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 3 cut(s) 395, 634, 916
PaeR7I CTCGAG 3 cut(s) 125, 237, 404
PaqCI CACCTGC 1 cut(s) 820
PciSI GCTCTTC 1 cut(s) 254
PdmI GAANNNNTTC 1 cut(s) 120
PfeI GAWTC 3 cut(s) 35, 325, 495
PflMI CCANNNNNTGG 1 cut(s) 267
PkrI GCNGC 3 cut(s) 7, 50, 451
PleI GAGTC 3 cut(s) 195, 402, 514
PpsI GAGTC 3 cut(s) 195, 402, 514
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 382
PspEI GGTNACC 1 cut(s) 143
PspGI CCWGG 1 cut(s) 382
PspN4I GGNNCC 3 cut(s) 658, 667, 679
PspPI GGNCC 3 cut(s) 54, 415, 929
PstNI CAGNNNCTG 1 cut(s) 190
PsuI RGATCY 1 cut(s) 859
RsaI GTAC 2 cut(s) 173, 679
RsaNI GTAC 2 cut(s) 172, 678
RseI CAYNNNNRTG 1 cut(s) 62
SacI GAGCTC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 254
SaqAI TTAA 2 cut(s) 708, 970
SatI GCNGC 3 cut(s) 6, 49, 450
Sau3AI GATC 5 cut(s) 346, 357, 369, 859, 870
Sau96I GGNCC 3 cut(s) 54, 415, 929
SchI GAGTC 3 cut(s) 196, 403, 514
ScrFI CCNGG 1 cut(s) 384
SduI GDGCHC 2 cut(s) 249, 581
SfaNI GCATC 3 cut(s) 92, 268, 888
Sfr274I CTCGAG 3 cut(s) 125, 237, 404
SinI GGWCC 2 cut(s) 54, 415
SlaI CTCGAG 3 cut(s) 125, 237, 404
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 4 cut(s) 125, 237, 320, 404
SmoI CTYRAG 4 cut(s) 125, 237, 320, 404
Sse9I AATT 5 cut(s) 91, 332, 502, 526, 608
SsiI CCGC 2 cut(s) 49, 148
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 382
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 1 cut(s) 739
TaiI ACGT 1 cut(s) 475
TaqI TCGA 7 cut(s) 126, 156, 238, 405, 693, 837, 904
TasI AATT 5 cut(s) 91, 332, 502, 526, 608
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 3 cut(s) 35, 325, 495
Tru1I TTAA 2 cut(s) 708, 970
Tru9I TTAA 2 cut(s) 708, 970
TscAI CASTG 3 cut(s) 27, 368, 379
TseFI GTSAC 3 cut(s) 395, 634, 916
TseI GCWGC 2 cut(s) 5, 449
Tsp45I GTSAC 3 cut(s) 395, 634, 916
TspDTI ATGAA 2 cut(s) 241, 726
TspRI CASTG 3 cut(s) 27, 368, 379
Van91I CCANNNNNTGG 1 cut(s) 267
VpaK11BI GGWCC 2 cut(s) 54, 415
XapI RAATTY 2 cut(s) 526, 608
XhoI CTCGAG 3 cut(s) 125, 237, 404
XmnI GAANNNNTTC 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.