Rroxscaffold_7G00172390

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013852
Physical Location & Seq
Reverse (-)
12627626 .. 12629569
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00172390.1

Sequence Viewer

Length: 450 bp
ATGTCGAGTAATATGTGGAAGAAGGCGAAACCCTATTTGCTGACGATAGGTTTGCAATTCGGAAGTGCAGGGATGTACATCATAAGCACTGCGACTCTCAACCATGGCATGAATCGATATGTTCTTATTGTGTATCGCAATGCTATTGCAGCTCTTGTCCTCGCTCCTTTTGCTTTGGTTCTTGAAAGGAAAACGAGGCCCAAAATGACGCTTTCAATCTTTTTCCACATAATGGTGCTGGCATTTCTAGAGCCGGTCCTTGATCAGGGCTTTGCTTACTTGGGAATGAAATATACTTCGGCATCATTTACTTCTGCCATCATGAATGCCGTTCCCTCTGTTACCTTTGTAATTTCCGTCATTTTTCGGATAGAGCATGTAAACATTAAGGAAGTCCGGAGTCAAGCGAAGGTGATAGGAACCCTAGTGACCTTTGGGGAGCTTTGTTGA

Protein Analysis

149

Amino Acids

16.59

Weight (kDa)

9.88

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 12 - 132 1.5e-11 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013852)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08290 AT4G08290
fragaria_vesca FvH4_2g26690 FvH4_2g26690
prunus_persica Prupe.1G500600_v2.0.a1
pyrus_communis pycom08g14200
rosa_chinensis RchiOBHm_Chr6g0295531
rosa_laevigata RLG00000011793
rosa_multiflora Rmu_sc0005000.1_g000013
rosa_roxburghii Rroxscaffold_7G00172390
rosa_rugosa Rorug06G0251700
rosa_samantha Rh2AG083600 Rh6AG364900 Rh6BG373300 Rh6CG379500 Rh6DG366000
rosa_wichuraiana Rw6G031870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 232
AccIII TCCGGA 1 cut(s) 396
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 2 cut(s) 232, 265
AgsI TTSAA 2 cut(s) 185, 216
AluBI AGCT 2 cut(s) 152, 442
AluI AGCT 2 cut(s) 152, 442
Aor13HI TCCGGA 1 cut(s) 396
AoxI GGCC 1 cut(s) 197
ApeKI GCWGC 1 cut(s) 149
ArsI GACNNNNNNTTYG 2 cut(s) 34, 66
AspS9I GGNCC 2 cut(s) 198, 256
AsuHPI GGTGA 1 cut(s) 424
AvaII GGWCC 1 cut(s) 256
BbvI GCAGC 1 cut(s) 161
BccI CCATC 1 cut(s) 326
BceAI ACGGC 1 cut(s) 314
BcgI CGANNNNNNTGC 2 cut(s) 34, 68
BclI TGATCA 1 cut(s) 262
BfaI CTAG 2 cut(s) 248, 425
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme18I GGWCC 1 cut(s) 256
BmgT120I GGNCC 2 cut(s) 198, 256
BmiI GGNNCC 1 cut(s) 421
BmsI GCATC 1 cut(s) 311
Bsa29I ATCGAT 1 cut(s) 115
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 103
BsaWI WCCGGW 1 cut(s) 396
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 265
Bse118I RCCGGY 1 cut(s) 253
Bse3DI GCAATG 1 cut(s) 145
Bse8I GATNNNNATC 1 cut(s) 77
BseAI TCCGGA 1 cut(s) 396
BseCI ATCGAT 1 cut(s) 115
BseDI CCNNGG 1 cut(s) 103
BseGI GGATG 1 cut(s) 78
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 2 cut(s) 232, 265
BseMI GCAATG 1 cut(s) 145
BseXI GCAGC 1 cut(s) 161
BsgI GTGCAG 1 cut(s) 87
BshFI GGCC 1 cut(s) 199
BshVI ATCGAT 1 cut(s) 115
BsiSI CCGG 2 cut(s) 254, 397
BslI CCNNNNNNNGG 2 cut(s) 232, 265
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 1 cut(s) 199
Bsp13I TCCGGA 1 cut(s) 396
Bsp1407I TGTACA 1 cut(s) 75
Bsp143I GATC 1 cut(s) 262
Bsp19I CCATGG 1 cut(s) 103
BspANI GGCC 1 cut(s) 199
BspDI ATCGAT 1 cut(s) 115
BspEI TCCGGA 1 cut(s) 396
BspHI TCATGA 1 cut(s) 321
BspLI GGNNCC 1 cut(s) 421
BsrDI GCAATG 1 cut(s) 145
BsrFI RCCGGY 1 cut(s) 253
BsrGI TGTACA 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 253
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 1 cut(s) 262
BssT1I CCWWGG 1 cut(s) 103
BstAUI TGTACA 1 cut(s) 75
BstC8I GCNNGC 1 cut(s) 240
BstDSI CCRYGG 1 cut(s) 103
BstENI CCTNNNNNAGG 1 cut(s) 263
BstF5I GGATG 1 cut(s) 78
BstKTI GATC 1 cut(s) 265
BstMBI GATC 1 cut(s) 262
BstMWI GCNNNNNNNGC 2 cut(s) 149, 170
BstNSI RCATGY 1 cut(s) 380
BstV1I GCAGC 1 cut(s) 161
Bsu15I ATCGAT 1 cut(s) 115
BsuRI GGCC 1 cut(s) 199
BsuTUI ATCGAT 1 cut(s) 115
BtgI CCRYGG 1 cut(s) 103
BtsCI GGATG 1 cut(s) 78
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 1 cut(s) 240
CciI TCATGA 1 cut(s) 321
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 2 cut(s) 198, 256
ClaI ATCGAT 1 cut(s) 115
CseI GACGC 1 cut(s) 217
Csp6I GTAC 1 cut(s) 76
CviAII CATG 4 cut(s) 104, 109, 322, 377
CviJI RGCY 5 cut(s) 152, 199, 253, 270, 442
CviKI_1 RGCY 5 cut(s) 152, 199, 253, 270, 442
CviQI GTAC 1 cut(s) 76
DpnI GATC 1 cut(s) 264
DpnII GATC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 103
Eco47I GGWCC 1 cut(s) 256
EcoNI CCTNNNNNAGG 1 cut(s) 263
EcoT14I CCWWGG 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 4 cut(s) 107, 112, 325, 380
FaiI YATR 9 cut(s) 14, 83, 105, 110, 120, 230, 294, 323, 378
FatI CATG 4 cut(s) 103, 108, 321, 376
FbaI TGATCA 1 cut(s) 262
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 1 cut(s) 85
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 2 cut(s) 248, 425
GluI GCNGC 1 cut(s) 150
HaeIII GGCC 1 cut(s) 199
HapII CCGG 2 cut(s) 254, 397
HgaI GACGC 1 cut(s) 217
Hin1II CATG 4 cut(s) 107, 112, 325, 380
HinfI GANTC 3 cut(s) 94, 112, 400
HpaII CCGG 2 cut(s) 254, 397
HphI GGTGA 1 cut(s) 424
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 2 cut(s) 62, 369
Hpy188III TCNNGA 4 cut(s) 182, 248, 322, 397
Hpy8I GTNNAC 1 cut(s) 382
HpyAV CCTTC 2 cut(s) 16, 403
HpyCH4V TGCA 3 cut(s) 55, 68, 149
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 170
Hsp92II CATG 4 cut(s) 107, 112, 325, 380
Kpn2I TCCGGA 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 1 cut(s) 262
LmnI GCTCC 2 cut(s) 169, 439
LpnPI CCDG 5 cut(s) 54, 224, 251, 267, 410
Lsp1109I GCAGC 1 cut(s) 161
LweI GCATC 1 cut(s) 311
MaeI CTAG 2 cut(s) 248, 425
MaeIII GTNAC 2 cut(s) 340, 427
MalI GATC 1 cut(s) 264
MboI GATC 1 cut(s) 262
MboII GAAGA 1 cut(s) 31
MluCI AATT 2 cut(s) 56, 351
MlyI GAGTC 2 cut(s) 88, 409
MnlI CCTC 3 cut(s) 170, 189, 346
MroI TCCGGA 1 cut(s) 396
MseI TTAA 1 cut(s) 387
MslI CAYNNNNRTG 1 cut(s) 233
MspI CCGG 2 cut(s) 254, 397
Mva1269I GAATGC 1 cut(s) 331
MwoI GCNNNNNNNGC 2 cut(s) 149, 170
NcoI CCATGG 1 cut(s) 103
NdeII GATC 1 cut(s) 262
NlaIII CATG 4 cut(s) 107, 112, 325, 380
NlaIV GGNNCC 1 cut(s) 421
NmuCI GTSAC 1 cut(s) 427
NspI RCATGY 1 cut(s) 380
PagI TCATGA 1 cut(s) 321
PctI GAATGC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 112
PflMI CCANNNNNTGG 1 cut(s) 232
PkrI GCNGC 1 cut(s) 151
PleI GAGTC 2 cut(s) 88, 408
PpsI GAGTC 2 cut(s) 88, 408
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 2 cut(s) 198, 256
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
RseI CAYNNNNRTG 1 cut(s) 233
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 1 cut(s) 262
Sau96I GGNCC 2 cut(s) 198, 256
SchI GAGTC 2 cut(s) 88, 409
SetI ASST 6 cut(s) 52, 154, 347, 414, 434, 444
SfaNI GCATC 1 cut(s) 311
SinI GGWCC 1 cut(s) 256
SmiMI CAYNNNNRTG 1 cut(s) 233
Sse9I AATT 2 cut(s) 56, 351
SspMI CTAG 2 cut(s) 248, 425
StyI CCWWGG 1 cut(s) 103
TaqI TCGA 2 cut(s) 5, 115
TasI AATT 2 cut(s) 56, 351
TatI WGTACW 1 cut(s) 75
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 1 cut(s) 94
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 3 cut(s) 125, 302, 338
TspGWI ACGGA 1 cut(s) 346
TspRI CASTG 1 cut(s) 94
Van91I CCANNNNNTGG 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 256
XagI CCTNNNNNAGG 1 cut(s) 263
XbaI TCTAGA 1 cut(s) 247
XceI RCATGY 1 cut(s) 380
XspI CTAG 2 cut(s) 248, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.