Rh6BG373300

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0013852
Physical Location & Seq
Forward (+)
60260358 .. 60263895
3538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG373300.1

Sequence Viewer

Length: 867 bp
ATGAAATATACTTCGGCATCATTTACTTCTGCCATCATGAATGCCGTTCCCTCTGTTACCTTTGTAATTTCAGTCATTTTCCGGATAGAGCATGTAAACATTAAGGAAGTCCGGAGTCAAGCGAAGGTGATAGGAACCCTAGTGACCTTTGGGGGAGCTTTGTTGATGGCAATGTACAAAGGCCCTGCCATTAACTTAGTAGGCTCACATGATGACGGAGGCCACCATGAAAGTAGCAGCCGCACCTCGATCACACACTGGGCAATGGGGACGCTCTTTATCCTCATAGGTTGTGTGGCTTGGTCCTGCTTCTACGTGTTACAATCTGTAACACTGAAGAAATACCCGGCAAACATATCCCTTTCCTGCTGGATATGCTCGATGGGCGCAATGCAAGGTGCAGCAGTAGCGTTTGTTGCAGAGCGCCATCCTAGTGCTTGGGCCGTCGGCTGGGATACGAGGCTCCTAGCACCTCTCTATACAGGAATAGTTAGCTCTGGAATTACATACTATGTGCAAGGGTTGGTAATGAAAACTAGAGGACCAGTATTTGTAACAGCTTTTAACCCTCTCTGCATGATCCTCGTCGCAATTCTGGCTTCGTTCGTTCTAGCTGAGAAACTCCACCTGGGCAGTATCATTGGAGGCTTTATAATCGCCATCGGCCTTTACGCTGTGGTGTGGGGTAAAAGCAAGGACTATAGTACTGGTACTAGCCCAACCAGTGATCCCACTGATCTCAAGGATGAGAATGAAGCTCACAAATTACCAATTACTACAACCAATGGGACTAAACTGACCATTATTGGCGACAAAAATAGCAGCAACCAGCCAGCAGAGATGCAATTTATGACGGTCCAAAAATAA

Protein Analysis

288

Amino Acids

31.02

Weight (kDa)

8.91

Isoelectric Point (pI)

31.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 89 - 227 6.6e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013852)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08290 AT4G08290
fragaria_vesca FvH4_2g26690 FvH4_2g26690
prunus_persica Prupe.1G500600_v2.0.a1
pyrus_communis pycom08g14200
rosa_chinensis RchiOBHm_Chr6g0295531
rosa_laevigata RLG00000011793
rosa_multiflora Rmu_sc0005000.1_g000013
rosa_roxburghii Rroxscaffold_7G00172390
rosa_rugosa Rorug06G0251700
rosa_samantha Rh2AG083600 Rh6AG364900 Rh6BG373300 Rh6CG379500 Rh6DG366000
rosa_wichuraiana Rw6G031870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 653
AccIII TCCGGA 2 cut(s) 81, 111
AciI CCGC 1 cut(s) 241
AclWI GGATC 2 cut(s) 574, 722
AcuI CTGAAG 1 cut(s) 356
AfaI GTAC 3 cut(s) 176, 706, 712
AfiI CCNNNNNNNGG 1 cut(s) 450
AflIII ACRYGT 1 cut(s) 315
AjnI CCWGG 1 cut(s) 627
AluBI AGCT 5 cut(s) 158, 495, 560, 614, 758
AluI AGCT 5 cut(s) 158, 495, 560, 614, 758
AlwI GGATC 2 cut(s) 574, 722
Aor13HI TCCGGA 2 cut(s) 81, 111
AoxI GGCC 4 cut(s) 181, 220, 441, 664
ApeKI GCWGC 3 cut(s) 237, 401, 822
AspLEI GCGC 2 cut(s) 389, 426
AspS9I GGNCC 5 cut(s) 182, 303, 441, 542, 856
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 1 cut(s) 139
AvaII GGWCC 3 cut(s) 303, 542, 856
BbvI GCAGC 3 cut(s) 249, 413, 834
BccI CCATC 5 cut(s) 41, 160, 376, 435, 668
BceAI ACGGC 2 cut(s) 29, 428
BcgI CGANNNNNNTGC 2 cut(s) 565, 599
BciT130I CCWGG 1 cut(s) 629
BciVI GTATCC 1 cut(s) 448
BcnI CCSGG 1 cut(s) 347
BfaI CTAG 6 cut(s) 140, 432, 467, 537, 611, 714
BfmI CTRYAG 1 cut(s) 700
BfoI RGCGCY 1 cut(s) 427
BfuI GTATCC 1 cut(s) 448
BisI GCNGC 4 cut(s) 238, 241, 402, 823
BlsI GCNGC 4 cut(s) 239, 242, 403, 824
BmcAI AGTACT 1 cut(s) 706
Bme1390I CCNGG 2 cut(s) 347, 629
Bme18I GGWCC 3 cut(s) 303, 542, 856
BmgT120I GGNCC 5 cut(s) 182, 303, 441, 542, 856
BmiI GGNNCC 2 cut(s) 136, 464
BmrFI CCNGG 2 cut(s) 347, 629
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 2 cut(s) 26, 831
BmuI ACTGGG 1 cut(s) 268
BpuEI CTTGAG 1 cut(s) 725
BpuMI CCSGG 1 cut(s) 347
BsaAI YACGTR 1 cut(s) 316
BsaJI CCNNGG 1 cut(s) 628
BsaWI WCCGGW 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 1 cut(s) 450
Bse1I ACTGG 4 cut(s) 263, 545, 712, 723
Bse3DI GCAATG 3 cut(s) 177, 270, 396
BseAI TCCGGA 2 cut(s) 81, 111
BseBI CCWGG 1 cut(s) 629
BseDI CCNNGG 1 cut(s) 628
BseGI GGATG 2 cut(s) 427, 751
BseLI CCNNNNNNNGG 1 cut(s) 450
BseMI GCAATG 3 cut(s) 177, 270, 396
BseMII CTCAG 1 cut(s) 606
BseNI ACTGG 4 cut(s) 263, 545, 712, 723
BseXI GCAGC 3 cut(s) 249, 413, 834
BseYI CCCAGC 1 cut(s) 450
BsgI GTGCAG 1 cut(s) 420
BshFI GGCC 4 cut(s) 183, 222, 443, 666
BsiSI CCGG 3 cut(s) 82, 112, 347
BslFI GGGAC 2 cut(s) 283, 802
BslI CCNNNNNNNGG 1 cut(s) 450
BsmFI GGGAC 2 cut(s) 283, 802
BsmI GAATGC 1 cut(s) 46
BsnI GGCC 4 cut(s) 183, 222, 443, 666
Bsp13I TCCGGA 2 cut(s) 81, 111
Bsp1407I TGTACA 1 cut(s) 174
Bsp143I GATC 4 cut(s) 249, 579, 727, 736
BspACI CCGC 1 cut(s) 241
BspANI GGCC 4 cut(s) 183, 222, 443, 666
BspCNI CTCAG 1 cut(s) 607
BspEI TCCGGA 2 cut(s) 81, 111
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 2 cut(s) 136, 464
BspPI GGATC 2 cut(s) 574, 722
BsrDI GCAATG 3 cut(s) 177, 270, 396
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 4 cut(s) 263, 545, 712, 723
BssECI CCNNGG 1 cut(s) 628
BssMI GATC 4 cut(s) 249, 579, 727, 736
Bst2UI CCWGG 1 cut(s) 629
Bst4CI ACNGT 1 cut(s) 856
BstAUI TGTACA 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 316
BstC8I GCNNGC 1 cut(s) 834
BstDEI CTNAG 2 cut(s) 196, 615
BstF5I GGATG 2 cut(s) 427, 751
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 2 cut(s) 389, 426
BstKTI GATC 4 cut(s) 252, 582, 730, 739
BstMBI GATC 4 cut(s) 249, 579, 727, 736
BstMWI GCNNNNNNNGC 5 cut(s) 375, 384, 407, 416, 596
BstNI CCWGG 1 cut(s) 629
BstNSI RCATGY 1 cut(s) 95
BstSCI CCNGG 2 cut(s) 345, 627
BstSFI CTRYAG 1 cut(s) 700
BstV1I GCAGC 3 cut(s) 249, 413, 834
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 4 cut(s) 183, 222, 443, 666
BtsCI GGATG 2 cut(s) 427, 751
BtsIMutI CAGTG 4 cut(s) 256, 332, 730, 732
Cac8I GCNNGC 1 cut(s) 834
CciI TCATGA 1 cut(s) 36
CfoI GCGC 2 cut(s) 389, 426
Cfr13I GGNCC 5 cut(s) 182, 303, 441, 542, 856
CseI GACGC 1 cut(s) 280
Csp6I GTAC 3 cut(s) 175, 705, 711
CviAII CATG 5 cut(s) 37, 92, 209, 227, 577
CviQI GTAC 3 cut(s) 175, 705, 711
DdeI CTNAG 2 cut(s) 196, 615
DpnI GATC 4 cut(s) 251, 581, 729, 738
DpnII GATC 4 cut(s) 249, 579, 727, 736
Eco47I GGWCC 3 cut(s) 303, 542, 856
Eco57I CTGAAG 1 cut(s) 356
EcoO109I RGGNCCY 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 627
FaeI CATG 5 cut(s) 40, 95, 212, 230, 580
FaqI GGGAC 2 cut(s) 283, 802
FatI CATG 5 cut(s) 36, 91, 208, 226, 576
Fnu4HI GCNGC 4 cut(s) 238, 241, 402, 823
FokI GGATG 2 cut(s) 414, 758
Fsp4HI GCNGC 4 cut(s) 238, 241, 402, 823
FspBI CTAG 6 cut(s) 140, 432, 467, 537, 611, 714
GlaI GCGC 2 cut(s) 388, 425
GluI GCNGC 4 cut(s) 238, 241, 402, 823
GsaI CCCAGC 1 cut(s) 454
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 4 cut(s) 183, 222, 443, 666
HapII CCGG 3 cut(s) 82, 112, 347
HgaI GACGC 1 cut(s) 280
HhaI GCGC 2 cut(s) 389, 426
Hin1II CATG 5 cut(s) 40, 95, 212, 230, 580
Hin6I GCGC 2 cut(s) 387, 424
HinP1I GCGC 2 cut(s) 387, 424
HinfI GANTC 1 cut(s) 115
HpaII CCGG 3 cut(s) 82, 112, 347
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 1 cut(s) 97
Hpy188III TCNNGA 4 cut(s) 37, 82, 112, 498
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 2 cut(s) 449, 590
HpyAV CCTTC 1 cut(s) 118
HpyCH4III ACNGT 1 cut(s) 856
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 6 cut(s) 394, 401, 419, 517, 576, 844
HpyF10VI GCNNNNNNNGC 5 cut(s) 375, 384, 407, 416, 596
HpyF3I CTNAG 2 cut(s) 196, 615
HpySE526I ACGT 1 cut(s) 315
Hsp92II CATG 5 cut(s) 40, 95, 212, 230, 580
HspAI GCGC 2 cut(s) 387, 424
Kpn2I TCCGGA 2 cut(s) 81, 111
Kzo9I GATC 4 cut(s) 249, 579, 727, 736
LmnI GCTCC 2 cut(s) 155, 468
Lsp1109I GCAGC 3 cut(s) 249, 413, 834
LweI GCATC 2 cut(s) 26, 831
MaeI CTAG 6 cut(s) 140, 432, 467, 537, 611, 714
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 5 cut(s) 55, 142, 318, 328, 553
MalI GATC 4 cut(s) 251, 581, 729, 738
MboI GATC 4 cut(s) 249, 579, 727, 736
MboII GAAGA 1 cut(s) 349
MluCI AATT 6 cut(s) 66, 501, 591, 764, 771, 845
MlyI GAGTC 1 cut(s) 124
MroI TCCGGA 2 cut(s) 81, 111
MseI TTAA 3 cut(s) 102, 192, 564
MslI CAYNNNNRTG 1 cut(s) 432
MspI CCGG 3 cut(s) 82, 112, 347
MspR9I CCNGG 2 cut(s) 347, 629
Mva1269I GAATGC 1 cut(s) 46
MvaI CCWGG 1 cut(s) 629
MwoI GCNNNNNNNGC 5 cut(s) 375, 384, 407, 416, 596
NciI CCSGG 1 cut(s) 347
NdeII GATC 4 cut(s) 249, 579, 727, 736
NlaIII CATG 5 cut(s) 40, 95, 212, 230, 580
NlaIV GGNNCC 2 cut(s) 136, 464
NmuCI GTSAC 1 cut(s) 142
NspI RCATGY 1 cut(s) 95
PagI TCATGA 1 cut(s) 36
PctI GAATGC 1 cut(s) 46
PkrI GCNGC 4 cut(s) 239, 242, 403, 824
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
Ppu21I YACGTR 1 cut(s) 316
PsiI TTATAA 1 cut(s) 653
Psp6I CCWGG 1 cut(s) 627
PspFI CCCAGC 1 cut(s) 450
PspGI CCWGG 1 cut(s) 627
PspN4I GGNNCC 2 cut(s) 136, 464
PspPI GGNCC 5 cut(s) 182, 303, 441, 542, 856
RsaI GTAC 3 cut(s) 176, 706, 712
RsaNI GTAC 3 cut(s) 175, 705, 711
RseI CAYNNNNRTG 1 cut(s) 432
SaqAI TTAA 3 cut(s) 102, 192, 564
SatI GCNGC 4 cut(s) 238, 241, 402, 823
Sau3AI GATC 4 cut(s) 249, 579, 727, 736
Sau96I GGNCC 5 cut(s) 182, 303, 441, 542, 856
ScaI AGTACT 1 cut(s) 706
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 2 cut(s) 347, 629
SfaNI GCATC 2 cut(s) 26, 831
SfcI CTRYAG 1 cut(s) 700
SinI GGWCC 3 cut(s) 303, 542, 856
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 6 cut(s) 66, 501, 591, 764, 771, 845
SsiI CCGC 1 cut(s) 241
SspMI CTAG 6 cut(s) 140, 432, 467, 537, 611, 714
StyD4I CCNGG 2 cut(s) 345, 627
TaaI ACNGT 1 cut(s) 856
TaiI ACGT 1 cut(s) 318
TaqI TCGA 2 cut(s) 248, 380
TasI AATT 6 cut(s) 66, 501, 591, 764, 771, 845
TatI WGTACW 2 cut(s) 174, 704
TauI GCSGC 1 cut(s) 243
Tru1I TTAA 3 cut(s) 102, 192, 564
Tru9I TTAA 3 cut(s) 102, 192, 564
TscAI CASTG 4 cut(s) 263, 339, 730, 739
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 3 cut(s) 237, 401, 822
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 5 cut(s) 17, 53, 243, 545, 768
TspGWI ACGGA 1 cut(s) 231
TspRI CASTG 4 cut(s) 263, 339, 730, 739
VpaK11BI GGWCC 3 cut(s) 303, 542, 856
XceI RCATGY 1 cut(s) 95
XspI CTAG 6 cut(s) 140, 432, 467, 537, 611, 714
ZrmI AGTACT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.