Rh6DG366000

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013852
Physical Location & Seq
Forward (+)
56276242 .. 56279765
3524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG366000.1

Sequence Viewer

Length: 849 bp
ATGTCGAGTAATATGTGGAAGAACGCGAAACCCTATTTGCTGACGATAGGTTTGCAATTCGGAAGTGCAGGGATGTACATCATAAGCGCTGCGACTCTCAACCATGGCATGAATCGATATGTTCTTATTGTGTATCGCAATGCTATTGCAGCTCTTGTCCTCGCTCCTTTTGCTTTGGTTCTTGAAAGGAAAACGAGGCCCAAAATGACGCTTTCAATCTTTTTCCACATAATGGTGCTGGGATTTCTAGAGCCGGTCCTTGATCAGGGCTTTGCTTACTTGGGAATGAAATATACTTCGGCATCATTTACTTCTGCCATCATGAATGCCGTTCCCTCTGTTACCTTTGTAATTTCCGTCATTTTCCGGATAGAGCATGTAAACATTAAGGAAGTCCGGAGTCAAGCGAAGGTGATAGGAACCCTAGTGACCTTTGGGGGAGCTTTGTTGATGGCAATGTACAAAGGCCCTGCCATTAACTTAGTAGGCTCACATGATGACGGAGGCCACCATGAAAGTAGCAGCCGCACCTCGAGCACACACTGGGCAATGGGGACGGTCTTTATCCTCATAGGTTGTGTGGCTTGGTCCTGCTTCTACGTGTTACAATCTGTAACACTGAAGAAATACCCGGCAAACATATCCCTTTCCTGCTGGATATGCTCGATGGGCGCAATGCAAGGTGCAGCAGTAGCGTTTGTTGCAGAGCGCCATCCTAGTGCTTGGGCCGTCGGCTGGGATACGAGGCTCCTAGCACCTCTCTATACAGTTAGTAACACGCACATTAAGAATATATATGGAACGTCACAACAATTAGTTTTGTGTGACATCTTTGCTCGTTCACAATGA

Protein Analysis

282

Amino Acids

30.92

Weight (kDa)

9.49

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 12 - 152 1.4e-11 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013852)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08290 AT4G08290
fragaria_vesca FvH4_2g26690 FvH4_2g26690
prunus_persica Prupe.1G500600_v2.0.a1
pyrus_communis pycom08g14200
rosa_chinensis RchiOBHm_Chr6g0295531
rosa_laevigata RLG00000011793
rosa_multiflora Rmu_sc0005000.1_g000013
rosa_roxburghii Rroxscaffold_7G00172390
rosa_rugosa Rorug06G0251700
rosa_samantha Rh2AG083600 Rh6AG364900 Rh6BG373300 Rh6CG379500 Rh6DG366000
rosa_wichuraiana Rw6G031870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 232
AccII CGCG 1 cut(s) 26
AccIII TCCGGA 2 cut(s) 366, 396
AciI CCGC 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 641
AfaI GTAC 2 cut(s) 77, 461
AfeI AGCGCT 1 cut(s) 88
AfiI CCNNNNNNNGG 3 cut(s) 232, 265, 735
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 2 cut(s) 185, 216
AluBI AGCT 2 cut(s) 152, 443
AluI AGCT 2 cut(s) 152, 443
Alw21I GWGCWC 1 cut(s) 539
Ama87I CYCGRG 1 cut(s) 532
Aor13HI TCCGGA 2 cut(s) 366, 396
Aor51HI AGCGCT 1 cut(s) 88
AoxI GGCC 4 cut(s) 197, 466, 505, 726
ApeKI GCWGC 4 cut(s) 89, 149, 522, 686
ArsI GACNNNNNNTTYG 2 cut(s) 34, 66
AspLEI GCGC 3 cut(s) 89, 674, 711
AspS9I GGNCC 5 cut(s) 198, 256, 467, 588, 726
AsuC2I CCSGG 1 cut(s) 632
AsuHPI GGTGA 1 cut(s) 424
AvaI CYCGRG 1 cut(s) 532
AvaII GGWCC 2 cut(s) 256, 588
Bbv12I GWGCWC 1 cut(s) 539
BbvI GCAGC 4 cut(s) 76, 161, 534, 698
BccI CCATC 4 cut(s) 326, 445, 661, 720
BceAI ACGGC 2 cut(s) 314, 713
BcgI CGANNNNNNTGC 2 cut(s) 34, 68
BciVI GTATCC 1 cut(s) 733
BclI TGATCA 1 cut(s) 262
BcnI CCSGG 1 cut(s) 632
BfaI CTAG 4 cut(s) 248, 425, 717, 752
BfoI RGCGCY 2 cut(s) 90, 712
BfuI GTATCC 1 cut(s) 733
BisI GCNGC 5 cut(s) 90, 150, 523, 526, 687
BlsI GCNGC 5 cut(s) 91, 151, 524, 527, 688
Bme1390I CCNGG 1 cut(s) 632
Bme18I GGWCC 2 cut(s) 256, 588
BmeT110I CYCGRG 1 cut(s) 532
BmgT120I GGNCC 5 cut(s) 198, 256, 467, 588, 726
BmiI GGNNCC 2 cut(s) 421, 749
BmrFI CCNGG 1 cut(s) 632
BmrI ACTGGG 1 cut(s) 553
BmsI GCATC 1 cut(s) 311
BmuI ACTGGG 1 cut(s) 553
BpuMI CCSGG 1 cut(s) 632
Bsa29I ATCGAT 1 cut(s) 115
BsaAI YACGTR 1 cut(s) 601
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 103
BsaWI WCCGGW 2 cut(s) 366, 396
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 265, 735
Bse118I RCCGGY 1 cut(s) 253
Bse1I ACTGG 1 cut(s) 548
Bse3DI GCAATG 4 cut(s) 145, 462, 555, 681
Bse8I GATNNNNATC 1 cut(s) 77
BseAI TCCGGA 2 cut(s) 366, 396
BseCI ATCGAT 1 cut(s) 115
BseDI CCNNGG 1 cut(s) 103
BseGI GGATG 2 cut(s) 78, 712
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 3 cut(s) 232, 265, 735
BseMI GCAATG 4 cut(s) 145, 462, 555, 681
BseNI ACTGG 1 cut(s) 548
BseXI GCAGC 4 cut(s) 76, 161, 534, 698
BseYI CCCAGC 2 cut(s) 238, 735
BsgI GTGCAG 2 cut(s) 87, 705
Bsh1236I CGCG 1 cut(s) 26
BshFI GGCC 4 cut(s) 199, 468, 507, 728
BshVI ATCGAT 1 cut(s) 115
BsiHKAI GWGCWC 1 cut(s) 539
BsiHKCI CYCGRG 1 cut(s) 532
BsiSI CCGG 4 cut(s) 254, 367, 397, 632
BslFI GGGAC 1 cut(s) 568
BslI CCNNNNNNNGG 3 cut(s) 232, 265, 735
BsmFI GGGAC 1 cut(s) 568
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 4 cut(s) 199, 468, 507, 728
BsoBI CYCGRG 1 cut(s) 532
Bsp1286I GDGCHC 1 cut(s) 539
Bsp13I TCCGGA 2 cut(s) 366, 396
Bsp1407I TGTACA 2 cut(s) 75, 459
Bsp143I GATC 1 cut(s) 262
Bsp19I CCATGG 1 cut(s) 103
BspACI CCGC 1 cut(s) 526
BspANI GGCC 4 cut(s) 199, 468, 507, 728
BspDI ATCGAT 1 cut(s) 115
BspEI TCCGGA 2 cut(s) 366, 396
BspFNI CGCG 1 cut(s) 26
BspHI TCATGA 1 cut(s) 321
BspLI GGNNCC 2 cut(s) 421, 749
BsrDI GCAATG 4 cut(s) 145, 462, 555, 681
BsrFI RCCGGY 1 cut(s) 253
BsrGI TGTACA 2 cut(s) 75, 459
BsrI ACTGG 1 cut(s) 548
BssAI RCCGGY 1 cut(s) 253
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 1 cut(s) 262
BssT1I CCWWGG 1 cut(s) 103
Bst4CI ACNGT 2 cut(s) 559, 769
BstAUI TGTACA 2 cut(s) 75, 459
BstBAI YACGTR 1 cut(s) 601
BstDEI CTNAG 1 cut(s) 481
BstDSI CCRYGG 1 cut(s) 103
BstENI CCTNNNNNAGG 1 cut(s) 263
BstF5I GGATG 2 cut(s) 78, 712
BstFNI CGCG 1 cut(s) 26
BstH2I RGCGCY 2 cut(s) 90, 712
BstHHI GCGC 3 cut(s) 89, 674, 711
BstKTI GATC 1 cut(s) 265
BstMBI GATC 1 cut(s) 262
BstMWI GCNNNNNNNGC 7 cut(s) 149, 170, 534, 660, 669, 692, 701
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 1 cut(s) 630
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 4 cut(s) 76, 161, 534, 698
Bsu15I ATCGAT 1 cut(s) 115
BsuI GTATCC 1 cut(s) 733
BsuRI GGCC 4 cut(s) 199, 468, 507, 728
BsuTUI ATCGAT 1 cut(s) 115
BtgI CCRYGG 1 cut(s) 103
BtsCI GGATG 2 cut(s) 78, 712
BtsIMutI CAGTG 2 cut(s) 541, 617
CciI TCATGA 1 cut(s) 321
CfoI GCGC 3 cut(s) 89, 674, 711
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 5 cut(s) 198, 256, 467, 588, 726
ClaI ATCGAT 1 cut(s) 115
CseI GACGC 1 cut(s) 217
Csp6I GTAC 2 cut(s) 76, 460
CviAII CATG 6 cut(s) 104, 109, 322, 377, 494, 512
CviQI GTAC 2 cut(s) 76, 460
DdeI CTNAG 1 cut(s) 481
DpnI GATC 1 cut(s) 264
DpnII GATC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 103
Eco47I GGWCC 2 cut(s) 256, 588
Eco47III AGCGCT 1 cut(s) 88
Eco57I CTGAAG 1 cut(s) 641
Eco88I CYCGRG 1 cut(s) 532
EcoNI CCTNNNNNAGG 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 6 cut(s) 107, 112, 325, 380, 497, 515
FaqI GGGAC 1 cut(s) 568
FatI CATG 6 cut(s) 103, 108, 321, 376, 493, 511
FbaI TGATCA 1 cut(s) 262
Fnu4HI GCNGC 5 cut(s) 90, 150, 523, 526, 687
FokI GGATG 2 cut(s) 85, 699
Fsp4HI GCNGC 5 cut(s) 90, 150, 523, 526, 687
FspBI CTAG 4 cut(s) 248, 425, 717, 752
GlaI GCGC 3 cut(s) 88, 673, 710
GluI GCNGC 5 cut(s) 90, 150, 523, 526, 687
GsaI CCCAGC 2 cut(s) 242, 739
HaeII RGCGCY 2 cut(s) 90, 712
HaeIII GGCC 4 cut(s) 199, 468, 507, 728
HapII CCGG 4 cut(s) 254, 367, 397, 632
HgaI GACGC 1 cut(s) 217
HhaI GCGC 3 cut(s) 89, 674, 711
Hin1II CATG 6 cut(s) 107, 112, 325, 380, 497, 515
Hin6I GCGC 3 cut(s) 87, 672, 709
HinP1I GCGC 3 cut(s) 87, 672, 709
HinfI GANTC 3 cut(s) 94, 112, 400
HpaII CCGG 4 cut(s) 254, 367, 397, 632
HphI GGTGA 1 cut(s) 424
Hpy166II GTNNAC 2 cut(s) 382, 842
Hpy188I TCNGA 1 cut(s) 62
Hpy188III TCNNGA 5 cut(s) 182, 248, 322, 367, 397
Hpy8I GTNNAC 2 cut(s) 382, 842
Hpy99I CGWCG 1 cut(s) 734
HpyAV CCTTC 1 cut(s) 403
HpyCH4III ACNGT 2 cut(s) 559, 769
HpyCH4IV ACGT 2 cut(s) 600, 803
HpyCH4V TGCA 6 cut(s) 55, 68, 149, 679, 686, 704
HpyF10VI GCNNNNNNNGC 7 cut(s) 149, 170, 534, 660, 669, 692, 701
HpyF3I CTNAG 1 cut(s) 481
HpySE526I ACGT 2 cut(s) 600, 803
Hsp92II CATG 6 cut(s) 107, 112, 325, 380, 497, 515
HspAI GCGC 3 cut(s) 87, 672, 709
Kpn2I TCCGGA 2 cut(s) 366, 396
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 1 cut(s) 262
LmnI GCTCC 3 cut(s) 169, 440, 753
Lsp1109I GCAGC 4 cut(s) 76, 161, 534, 698
LweI GCATC 1 cut(s) 311
MaeI CTAG 4 cut(s) 248, 425, 717, 752
MaeII ACGT 2 cut(s) 600, 803
MaeIII GTNAC 7 cut(s) 340, 427, 603, 613, 773, 804, 824
MalI GATC 1 cut(s) 264
MboI GATC 1 cut(s) 262
MboII GAAGA 2 cut(s) 31, 634
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 3 cut(s) 56, 351, 812
MlyI GAGTC 2 cut(s) 88, 409
MnlI CCTC 8 cut(s) 170, 189, 346, 497, 541, 578, 738, 768
MroI TCCGGA 2 cut(s) 366, 396
MseI TTAA 3 cut(s) 387, 477, 786
MslI CAYNNNNRTG 2 cut(s) 233, 717
MspI CCGG 4 cut(s) 254, 367, 397, 632
MspR9I CCNGG 1 cut(s) 632
Mva1269I GAATGC 1 cut(s) 331
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 7 cut(s) 149, 170, 534, 660, 669, 692, 701
NciI CCSGG 1 cut(s) 632
NcoI CCATGG 1 cut(s) 103
NdeII GATC 1 cut(s) 262
NlaIII CATG 6 cut(s) 107, 112, 325, 380, 497, 515
NlaIV GGNNCC 2 cut(s) 421, 749
NmuCI GTSAC 3 cut(s) 427, 804, 824
NspI RCATGY 1 cut(s) 380
PaeR7I CTCGAG 1 cut(s) 532
PagI TCATGA 1 cut(s) 321
PctI GAATGC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 112
PflMI CCANNNNNTGG 1 cut(s) 232
PkrI GCNGC 5 cut(s) 91, 151, 524, 527, 688
PleI GAGTC 2 cut(s) 88, 408
PpsI GAGTC 2 cut(s) 88, 408
Ppu21I YACGTR 1 cut(s) 601
PspFI CCCAGC 2 cut(s) 238, 735
PspN4I GGNNCC 2 cut(s) 421, 749
PspPI GGNCC 5 cut(s) 198, 256, 467, 588, 726
PspXI VCTCGAGB 1 cut(s) 532
RsaI GTAC 2 cut(s) 77, 461
RsaNI GTAC 2 cut(s) 76, 460
RseI CAYNNNNRTG 2 cut(s) 233, 717
SaqAI TTAA 3 cut(s) 387, 477, 786
SatI GCNGC 5 cut(s) 90, 150, 523, 526, 687
Sau3AI GATC 1 cut(s) 262
Sau96I GGNCC 5 cut(s) 198, 256, 467, 588, 726
SchI GAGTC 2 cut(s) 88, 409
ScrFI CCNGG 1 cut(s) 632
SduI GDGCHC 1 cut(s) 539
SfaNI GCATC 1 cut(s) 311
Sfr274I CTCGAG 1 cut(s) 532
SinI GGWCC 2 cut(s) 256, 588
SlaI CTCGAG 1 cut(s) 532
SmiMI CAYNNNNRTG 2 cut(s) 233, 717
SmlI CTYRAG 1 cut(s) 532
SmoI CTYRAG 1 cut(s) 532
Sse9I AATT 3 cut(s) 56, 351, 812
SsiI CCGC 1 cut(s) 526
SspMI CTAG 4 cut(s) 248, 425, 717, 752
StyD4I CCNGG 1 cut(s) 630
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 2 cut(s) 559, 769
TaiI ACGT 2 cut(s) 603, 806
TaqI TCGA 4 cut(s) 5, 115, 533, 665
TasI AATT 3 cut(s) 56, 351, 812
TatI WGTACW 2 cut(s) 75, 459
TauI GCSGC 1 cut(s) 528
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 3 cut(s) 387, 477, 786
Tru9I TTAA 3 cut(s) 387, 477, 786
TscAI CASTG 2 cut(s) 548, 624
TseFI GTSAC 3 cut(s) 427, 804, 824
TseI GCWGC 4 cut(s) 89, 149, 522, 686
Tsp45I GTSAC 3 cut(s) 427, 804, 824
TspDTI ATGAA 4 cut(s) 125, 302, 338, 528
TspGWI ACGGA 2 cut(s) 346, 516
TspRI CASTG 2 cut(s) 548, 624
Van91I CCANNNNNTGG 1 cut(s) 232
VpaK11BI GGWCC 2 cut(s) 256, 588
XagI CCTNNNNNAGG 1 cut(s) 263
XbaI TCTAGA 1 cut(s) 247
XceI RCATGY 1 cut(s) 380
XhoI CTCGAG 1 cut(s) 532
XspI CTAG 4 cut(s) 248, 425, 717, 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.