FvH4_2g27360

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013997
Physical Location & Seq
Reverse (-)
21738156 .. 21740928
2773 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27360.t1

Sequence Viewer

Length: 1128 bp
ATGCGAACCGGTTTAGCCTCCGGTGTAGCCTCCATCCGAAACCTCTACAGAGCAGCTTATAGATCCATCTCCAGAAAATGGCAAAAATTTAAGAAGAAATCGGCTTCCGATGAAGAACCCAAAGTTGATCAAGTCCATAAGGTTGTTCCTGTTGAGGAGGAGGGCAAGGATGCTTCAACCCGAGACCAGAAAAGTATGAATGGACTTGAATATCAAAGCTTTGGATACAACAGCCCAGCAATGGGAGGAAATGATGCCGGTAGCCCTAAATCTTCGACCAACTTCTTCAAGCACTCGAGCGTCGATAATTTGTTCAACATGTCATGTCATAATCTCTCAAGATGTGGTACACGATCTGCGAGTCACACCCCTTCACGTTCTCACAAAAAGAGGACGACTCGGAAAAGCTGCCCCGCCTCACCGGAAACCTCCACCTCATCCGGGAGGAAAAGCTCTAACGACTCAGTTAGTACTACGCCGTTCTCAAGAAGTACTACACCCATCTCCAAAAGCGGGCCTCTCTTGAATTGCGAGAGCCGGAGGAGCACCACCATCATGTTTTCCAACTCCTCCGGCATGTTGAAGCCCCCGGCCATCGAGAAGCAGCTCGAGTGCACCTTGGAGGAGCTTTGCTTTGGATGCAATAAGAAGATGAAGGTTACAAGGGTGGTTGTCAAAGACACCGGGCAAATGGCTCAAGAGGAGGAATTGGTAACGATAAATGTGAAGCCGGGATGGAAGAAAGGAACGAAGATTACATTTGAAGGATTGGGAAACGAGAGACCTGGTGCATACCCAGCAGACATAATCTTTGTGATCGCTGAGAAGAGGCACCCTTTGTTTGTAAAAGAAGGAGATGACCTAGAGTTGACAGTAGAAATCTCATTGATCCAGGCCCTTACTGGTTGCACAATCTCAATTCCTCTTCTGGGTGGAGAAAATACGACATTGACAATCGAAGATATCATATACCCGGGCTACGAAAAGACCATTCCACACCAAGGTATGCCAATCGCTAAGGGGGAAGGGAAGAGAGGAAATCTGAAAGTCATGTTCCTGGTTGAGTTCCCTACTTCTCTAACACAGGAGCAAAGATCAGATGTTGTTACTATTTTAGAGGACTCTTGA

Protein Analysis

376

Amino Acids

41.21

Weight (kDa)

9.11

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 199 - 357 1.3e-37 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44160
fragaria_vesca FvH4_2g27360
malus_domestica MD00G1029000.v1.1 MD15G1345600.v1.1
prunus_persica Prupe.1G493700_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0296201
rosa_laevigata RLG00000011718
rosa_multiflora Rmu_sc0028042.1_g000003 Rmu_sc0035469.1_g000001
rosa_roxburghii Rroxscaffold_7G00171750 Rroxscaffold_7G00178310
rosa_rugosa Rorug06G0259400
rosa_samantha Rh6AG371600 Rh6BG379500 Rh6CG384800 Rh6DG372000
rosa_wichuraiana Rw6G032380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 831
AccB7I CCANNNNNTGG 1 cut(s) 78
AciI CCGC 2 cut(s) 414, 513
AclWI GGATC 2 cut(s) 57, 883
AcoI YGGCCR 1 cut(s) 591
AcsI RAATTY 1 cut(s) 86
AfaI GTAC 3 cut(s) 349, 472, 493
AfiI CCNNNNNNNGG 7 cut(s) 78, 241, 242, 441, 513, 929, 1001
AflIII ACRYGT 1 cut(s) 318
AgeI ACCGGT 1 cut(s) 8
AgsI TTSAA 7 cut(s) 177, 209, 289, 316, 526, 583, 764
AjnI CCWGG 3 cut(s) 784, 891, 1056
AluBI AGCT 6 cut(s) 56, 219, 408, 453, 607, 628
AluI AGCT 6 cut(s) 56, 219, 408, 453, 607, 628
Alw21I GWGCWC 2 cut(s) 548, 617
Alw26I GTCTC 2 cut(s) 177, 775
Alw44I GTGCAC 1 cut(s) 613
AlwI GGATC 2 cut(s) 57, 883
Ama87I CYCGRG 4 cut(s) 180, 295, 608, 973
AoxI GGCC 3 cut(s) 515, 591, 894
ApaLI GTGCAC 1 cut(s) 613
ApeKI GCWGC 3 cut(s) 53, 408, 604
ApoI RAATTY 1 cut(s) 86
ArsI GACNNNNNNTTYG 2 cut(s) 794, 826
AsiGI ACCGGT 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 515, 895
AsuC2I CCSGG 6 cut(s) 442, 590, 685, 732, 974, 975
AsuHPI GGTGA 1 cut(s) 411
AvaI CYCGRG 4 cut(s) 180, 295, 608, 973
BaeGI GKGCMC 1 cut(s) 617
BanI GGYRCC 1 cut(s) 831
Bbv12I GWGCWC 2 cut(s) 548, 617
BbvI GCAGC 3 cut(s) 65, 395, 616
BccI CCATC 6 cut(s) 41, 74, 509, 560, 602, 729
BceAI ACGGC 1 cut(s) 463
BciT130I CCWGG 3 cut(s) 786, 893, 1058
BciVI GTATCC 1 cut(s) 218
BclI TGATCA 1 cut(s) 127
BcnI CCSGG 6 cut(s) 442, 590, 685, 732, 974, 975
BcoDI GTCTC 2 cut(s) 177, 775
BfaI CTAG 1 cut(s) 863
BfmI CTRYAG 1 cut(s) 46
BfuI GTATCC 1 cut(s) 218
BisI GCNGC 3 cut(s) 54, 409, 605
BlsI GCNGC 3 cut(s) 55, 410, 606
BmcAI AGTACT 2 cut(s) 472, 493
Bme1390I CCNGG 9 cut(s) 442, 590, 685, 732, 786, 893, 974, 975, 1058
BmeT110I CYCGRG 4 cut(s) 180, 295, 608, 973
BmgT120I GGNCC 2 cut(s) 515, 895
BmiI GGNNCC 1 cut(s) 833
BmrFI CCNGG 9 cut(s) 442, 590, 685, 732, 786, 893, 974, 975, 1058
BmsI GCATC 3 cut(s) 160, 244, 629
BplI GAGNNNNNCTC 2 cut(s) 382, 414
BpmI CTGGAG 1 cut(s) 55
Bpu10I CCTNAGC 1 cut(s) 1017
BpuEI CTTGAG 3 cut(s) 322, 469, 681
BpuMI CCSGG 6 cut(s) 442, 590, 685, 732, 974, 975
BsaI GGTCTC 2 cut(s) 177, 775
BsaJI CCNNGG 4 cut(s) 588, 618, 973, 1000
BsaWI WCCGGW 3 cut(s) 8, 20, 421
Bsc4I CCNNNNNNNGG 7 cut(s) 78, 241, 242, 441, 513, 929, 1001
Bse118I RCCGGY 2 cut(s) 8, 257
Bse1I ACTGG 1 cut(s) 907
Bse3DI GCAATG 1 cut(s) 246
BseBI CCWGG 3 cut(s) 786, 893, 1058
BseDI CCNNGG 4 cut(s) 588, 618, 973, 1000
BseGI GGATG 5 cut(s) 33, 175, 437, 644, 740
BseLI CCNNNNNNNGG 7 cut(s) 78, 241, 242, 441, 513, 929, 1001
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 2 cut(s) 477, 813
BseNI ACTGG 1 cut(s) 907
BseRI GAGGAG 6 cut(s) 170, 173, 556, 559, 638, 716
BseSI GKGCMC 1 cut(s) 617
BseXI GCAGC 3 cut(s) 65, 395, 616
BseYI CCCAGC 2 cut(s) 235, 796
BshFI GGCC 3 cut(s) 517, 593, 896
BshNI GGYRCC 1 cut(s) 831
BshTI ACCGGT 1 cut(s) 8
BsiHKAI GWGCWC 2 cut(s) 548, 617
BsiHKCI CYCGRG 4 cut(s) 180, 295, 608, 973
BslI CCNNNNNNNGG 7 cut(s) 78, 241, 242, 441, 513, 929, 1001
BsmAI GTCTC 2 cut(s) 177, 775
BsnI GGCC 3 cut(s) 517, 593, 896
Bso31I GGTCTC 2 cut(s) 177, 775
BsoBI CYCGRG 4 cut(s) 180, 295, 608, 973
Bsp1286I GDGCHC 2 cut(s) 548, 617
Bsp143I GATC 6 cut(s) 62, 127, 353, 816, 888, 1094
BspACI CCGC 2 cut(s) 414, 513
BspANI GGCC 3 cut(s) 517, 593, 896
BspCNI CTCAG 2 cut(s) 476, 814
BspLI GGNNCC 1 cut(s) 833
BspPI GGATC 2 cut(s) 57, 883
BspT107I GGYRCC 1 cut(s) 831
BspTNI GGTCTC 2 cut(s) 177, 775
BsrDI GCAATG 1 cut(s) 246
BsrFI RCCGGY 2 cut(s) 8, 257
BsrI ACTGG 1 cut(s) 907
BssAI RCCGGY 2 cut(s) 8, 257
BssECI CCNNGG 4 cut(s) 588, 618, 973, 1000
BssMI GATC 6 cut(s) 62, 127, 353, 816, 888, 1094
BssT1I CCWWGG 2 cut(s) 618, 1000
Bst2UI CCWGG 3 cut(s) 786, 893, 1058
Bst4CI ACNGT 1 cut(s) 874
Bst6I CTCTTC 3 cut(s) 821, 930, 1025
BstC8I GCNNGC 1 cut(s) 515
BstDEI CTNAG 3 cut(s) 463, 822, 1017
BstF5I GGATG 5 cut(s) 33, 175, 437, 644, 740
BstKTI GATC 6 cut(s) 65, 130, 356, 819, 891, 1097
BstMAI GTCTC 2 cut(s) 177, 775
BstMBI GATC 6 cut(s) 62, 127, 353, 816, 888, 1094
BstMWI GCNNNNNNNGC 3 cut(s) 543, 639, 797
BstNI CCWGG 3 cut(s) 786, 893, 1058
BstNSI RCATGY 2 cut(s) 322, 580
BstSCI CCNGG 9 cut(s) 440, 588, 683, 730, 784, 891, 972, 973, 1056
BstSFI CTRYAG 1 cut(s) 46
BstSLI GKGCMC 1 cut(s) 617
BstV1I GCAGC 3 cut(s) 65, 395, 616
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BsuI GTATCC 1 cut(s) 218
BsuRI GGCC 3 cut(s) 517, 593, 896
BtsCI GGATG 5 cut(s) 33, 175, 437, 644, 740
Cac8I GCNNGC 1 cut(s) 515
Cfr10I RCCGGY 2 cut(s) 8, 257
Cfr13I GGNCC 2 cut(s) 515, 895
Cfr9I CCCGGG 1 cut(s) 973
CseI GACGC 1 cut(s) 289
CsiI ACCWGGT 1 cut(s) 784
Csp6I GTAC 3 cut(s) 348, 471, 492
CspAI ACCGGT 1 cut(s) 8
CviAII CATG 5 cut(s) 319, 324, 556, 577, 1051
CviQI GTAC 3 cut(s) 348, 471, 492
DdeI CTNAG 3 cut(s) 463, 822, 1017
DpnI GATC 6 cut(s) 64, 129, 355, 818, 890, 1096
DpnII GATC 6 cut(s) 62, 127, 353, 816, 888, 1094
EaeI YGGCCR 1 cut(s) 591
Eam1104I CTCTTC 3 cut(s) 821, 930, 1025
EarI CTCTTC 3 cut(s) 821, 930, 1025
Eco130I CCWWGG 2 cut(s) 618, 1000
Eco31I GGTCTC 2 cut(s) 177, 775
Eco32I GATATC 1 cut(s) 964
Eco88I CYCGRG 4 cut(s) 180, 295, 608, 973
EcoO109I RGGNCCY 1 cut(s) 895
EcoRII CCWGG 3 cut(s) 784, 891, 1056
EcoRV GATATC 1 cut(s) 964
EcoT14I CCWWGG 2 cut(s) 618, 1000
ErhI CCWWGG 2 cut(s) 618, 1000
FaeI CATG 5 cut(s) 322, 327, 559, 580, 1054
FatI CATG 5 cut(s) 318, 323, 555, 576, 1050
FauI CCCGC 2 cut(s) 421, 506
FbaI TGATCA 1 cut(s) 127
Fnu4HI GCNGC 3 cut(s) 54, 409, 605
FokI GGATG 5 cut(s) 20, 182, 424, 651, 747
Fsp4HI GCNGC 3 cut(s) 54, 409, 605
FspBI CTAG 1 cut(s) 863
GluI GCNGC 3 cut(s) 54, 409, 605
GsaI CCCAGC 2 cut(s) 239, 800
GsuI CTGGAG 1 cut(s) 55
HaeIII GGCC 3 cut(s) 517, 593, 896
HgaI GACGC 1 cut(s) 289
Hin1II CATG 5 cut(s) 322, 327, 559, 580, 1054
HincII GTYRAC 1 cut(s) 870
HindII GTYRAC 1 cut(s) 870
HindIII AAGCTT 1 cut(s) 217
HinfI GANTC 4 cut(s) 361, 397, 461, 1121
HphI GGTGA 1 cut(s) 411
Hpy166II GTNNAC 3 cut(s) 350, 615, 870
Hpy188I TCNGA 5 cut(s) 38, 109, 402, 1044, 1099
Hpy188III TCNNGA 7 cut(s) 72, 339, 486, 523, 598, 698, 1125
Hpy8I GTNNAC 3 cut(s) 350, 615, 870
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 5 cut(s) 381, 649, 758, 845, 1019
HpyCH4III ACNGT 1 cut(s) 874
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 4 cut(s) 615, 642, 791, 909
HpyF10VI GCNNNNNNNGC 3 cut(s) 543, 639, 797
HpyF3I CTNAG 3 cut(s) 463, 822, 1017
HpySE526I ACGT 1 cut(s) 376
Hsp92II CATG 5 cut(s) 322, 327, 559, 580, 1054
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 6 cut(s) 62, 127, 353, 816, 888, 1094
LmnI GCTCC 3 cut(s) 543, 625, 1087
Lsp1109I GCAGC 3 cut(s) 65, 395, 616
LweI GCATC 3 cut(s) 160, 244, 629
MabI ACCWGGT 1 cut(s) 784
MaeI CTAG 1 cut(s) 863
MaeII ACGT 1 cut(s) 376
MaeIII GTNAC 4 cut(s) 362, 658, 712, 1105
MalI GATC 6 cut(s) 64, 129, 355, 818, 890, 1096
MboI GATC 6 cut(s) 62, 127, 353, 816, 888, 1094
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 2 cut(s) 548, 617
MluCI AATT 5 cut(s) 86, 307, 526, 707, 918
MlyI GAGTC 4 cut(s) 370, 391, 455, 1115
MmeI TCCRAC 1 cut(s) 588
MseI TTAA 1 cut(s) 90
MslI CAYNNNNRTG 1 cut(s) 554
MspR9I CCNGG 9 cut(s) 442, 590, 685, 732, 786, 893, 974, 975, 1058
MvaI CCWGG 3 cut(s) 786, 893, 1058
MwoI GCNNNNNNNGC 3 cut(s) 543, 639, 797
NciI CCSGG 6 cut(s) 442, 590, 685, 732, 974, 975
NdeII GATC 6 cut(s) 62, 127, 353, 816, 888, 1094
NlaIII CATG 5 cut(s) 322, 327, 559, 580, 1054
NlaIV GGNNCC 1 cut(s) 833
NmuCI GTSAC 1 cut(s) 362
NspI RCATGY 2 cut(s) 322, 580
PaeR7I CTCGAG 2 cut(s) 295, 608
PciI ACATGT 1 cut(s) 318
PflMI CCANNNNNTGG 1 cut(s) 78
PfoI TCCNGGA 1 cut(s) 440
PinAI ACCGGT 1 cut(s) 8
PkrI GCNGC 3 cut(s) 55, 410, 606
PleI GAGTC 4 cut(s) 369, 391, 455, 1115
PpsI GAGTC 4 cut(s) 369, 391, 455, 1115
PscI ACATGT 1 cut(s) 318
Psp6I CCWGG 3 cut(s) 784, 891, 1056
PspFI CCCAGC 2 cut(s) 235, 796
PspGI CCWGG 3 cut(s) 784, 891, 1056
PspN4I GGNNCC 1 cut(s) 833
PspPI GGNCC 2 cut(s) 515, 895
PspXI VCTCGAGB 2 cut(s) 295, 608
PsrI GAACNNNNNNTAC 2 cut(s) 739, 771
PsuI RGATCY 1 cut(s) 62
RsaI GTAC 3 cut(s) 349, 472, 493
RsaNI GTAC 3 cut(s) 348, 471, 492
RseI CAYNNNNRTG 1 cut(s) 554
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 3 cut(s) 54, 409, 605
Sau3AI GATC 6 cut(s) 62, 127, 353, 816, 888, 1094
Sau96I GGNCC 2 cut(s) 515, 895
ScaI AGTACT 2 cut(s) 472, 493
SchI GAGTC 4 cut(s) 370, 391, 455, 1115
ScrFI CCNGG 9 cut(s) 442, 590, 685, 732, 786, 893, 974, 975, 1058
SduI GDGCHC 2 cut(s) 548, 617
SexAI ACCWGGT 1 cut(s) 784
SfaNI GCATC 3 cut(s) 160, 244, 629
SfcI CTRYAG 1 cut(s) 46
Sfr274I CTCGAG 2 cut(s) 295, 608
SlaI CTCGAG 2 cut(s) 295, 608
SmaI CCCGGG 1 cut(s) 975
SmiMI CAYNNNNRTG 1 cut(s) 554
SmlI CTYRAG 5 cut(s) 295, 337, 484, 608, 696
SmoI CTYRAG 5 cut(s) 295, 337, 484, 608, 696
Sse9I AATT 5 cut(s) 86, 307, 526, 707, 918
SsiI CCGC 2 cut(s) 414, 513
SspMI CTAG 1 cut(s) 863
StyD4I CCNGG 9 cut(s) 440, 588, 683, 730, 784, 891, 972, 973, 1056
StyI CCWWGG 2 cut(s) 618, 1000
TaaI ACNGT 1 cut(s) 874
TaiI ACGT 1 cut(s) 379
TaqI TCGA 6 cut(s) 275, 296, 303, 597, 609, 957
TasI AATT 5 cut(s) 86, 307, 526, 707, 918
TatI WGTACW 2 cut(s) 470, 491
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TseFI GTSAC 1 cut(s) 362
TseI GCWGC 3 cut(s) 53, 408, 604
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 3 cut(s) 126, 212, 668
TspMI CCCGGG 1 cut(s) 973
Van91I CCANNNNNTGG 1 cut(s) 78
VneI GTGCAC 1 cut(s) 613
XapI RAATTY 1 cut(s) 86
XceI RCATGY 2 cut(s) 322, 580
XcmI CCANNNNNNNNNTGG 1 cut(s) 899
XhoI CTCGAG 2 cut(s) 295, 608
XmaI CCCGGG 1 cut(s) 973
XspI CTAG 1 cut(s) 863
ZrmI AGTACT 2 cut(s) 472, 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.