MD00G1029000.v1.1

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0013997
Physical Location & Seq
Forward (+)
4758635 .. 4760615
1981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1029000.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGCGTCCTTTTATCCCTTCCATTCGGAATCTGTATATATCTACCTATAAATCCATTGTCAAGAAATGGCGACACATGAAAAAGAAATCATCCTCAGCTCCACAAGAGGAAGCCATAGTTGACCAATTTCGAGACAATGATGTGATTTTTGAGGAAGTGGGTGAGGATACAGAAAAGAAAACCATGCATGGATTGCACAGCTTTAGATGCATTAGCCTGCAAAGAATGCGACGCCCTGATCCATCAAGCCCTAGAGGTCTCAAAAGCGTGGACTGCATCTTTAACGTGTCTTCTCCTCGTTTCTCAAAGAGCTCAAGCCGAAAGAGTCGCACCCCTTCACCCTCCCTCAAGAAAACTAAGAGCCGGAAAAGCTGGCCCGATTCTGCGGTGCCCTCTCCGGCAACCCCCACTACGCCGGGGTGTACTACTAAAAGCGCGGACTGCACGCCCGCCCATCCCTTTCTCTCAAAAAGTGAGAGCCGGAAGATCCCTGCCCCCATCATGTTTTCTAACTCATCTGGGATGTTGAAACCCCCAGCCATTGAGAAGCAGCTTGAGTGCACCTTAGAGGAGCTGTGCTTTGGATGCCAGAAGAAGATGAAGGTCACAAGGGCTATCGTCAAAGACACCGGGCAATTGGCTCAAGAAGATGAATTGGTAACAATACAAGTGGAGCCAGGATGGAAGAAAGGAACAAAGATTACATTTGAAGGATTGGGAAATGAGAGACGAGGTGCATACCCAGCAGACATAATCTTTGTGATTTCTGAAAAACGACACCCTTTGTTCAGAAGAGAAGGAGATGACTTGGAGTTGGCACTTGAGATTCCATTGGTGGAGGCTCTCACCGGCTGCATCATCTCAATCCCTCTGCTGGGCGGAGACCAGATGAAGCTGACGATCGACGACATCATATATCCTGGCTATGAAAAGATCATATCCGGCCAAGGCATGGCACTCTCTAAAGATGAAGGAAAGAGAGGAAACTTGAAGGTCACGTTTCTGGTCGATTTTCCGACCGCCCTCACCCATGAACAACGATTGGATGTTCAAACCATTTTAGAGGATTATGATTGA

Protein Analysis

359

Amino Acids

40.12

Weight (kDa)

9.04

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 181 - 339 3.4e-37 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44160
fragaria_vesca FvH4_2g27360
malus_domestica MD00G1029000.v1.1 MD15G1345600.v1.1
prunus_persica Prupe.1G493700_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0296201
rosa_laevigata RLG00000011718
rosa_multiflora Rmu_sc0028042.1_g000003 Rmu_sc0035469.1_g000001
rosa_roxburghii Rroxscaffold_7G00171750 Rroxscaffold_7G00178310
rosa_rugosa Rorug06G0259400
rosa_samantha Rh6AG371600 Rh6BG379500 Rh6CG384800 Rh6DG372000
rosa_wichuraiana Rw6G032380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 388
AccB7I CCANNNNNTGG 1 cut(s) 952
AccII CGCG 1 cut(s) 437
AciI CCGC 5 cut(s) 386, 437, 450, 879, 1020
AclWI GGATC 2 cut(s) 233, 481
AcoI YGGCCR 1 cut(s) 943
AcyI GRCGYC 1 cut(s) 232
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 3 cut(s) 874, 875, 952
AflIII ACRYGT 1 cut(s) 285
AgsI TTSAA 4 cut(s) 529, 710, 991, 1052
AjnI CCWGG 2 cut(s) 676, 919
AluBI AGCT 7 cut(s) 98, 201, 312, 372, 553, 574, 895
AluI AGCT 7 cut(s) 98, 201, 312, 372, 553, 574, 895
Alw21I GWGCWC 2 cut(s) 314, 563
Alw26I GTCTC 4 cut(s) 126, 263, 721, 876
Alw44I GTGCAC 1 cut(s) 559
AlwI GGATC 2 cut(s) 233, 481
AoxI GGCC 2 cut(s) 374, 943
ApaLI GTGCAC 1 cut(s) 559
ApeKI GCWGC 2 cut(s) 550, 852
ArsI GACNNNNNNTTYG 2 cut(s) 740, 772
AspLEI GCGC 1 cut(s) 437
AspS9I GGNCC 1 cut(s) 375
AsuC2I CCSGG 2 cut(s) 417, 631
AsuHPI GGTGA 4 cut(s) 173, 330, 838, 1018
BaeGI GKGCMC 2 cut(s) 393, 563
BanI GGYRCC 1 cut(s) 388
BanII GRGCYC 1 cut(s) 314
BbsI GAAGAC 1 cut(s) 282
Bbv12I GWGCWC 2 cut(s) 314, 563
BbvCI CCTCAGC 1 cut(s) 94
BbvI GCAGC 2 cut(s) 562, 839
BccI CCATC 4 cut(s) 250, 462, 506, 675
BciT130I CCWGG 2 cut(s) 678, 921
BciVI GTATCC 1 cut(s) 160
BcnI CCSGG 2 cut(s) 417, 631
BcoDI GTCTC 4 cut(s) 126, 263, 721, 876
BfaI CTAG 1 cut(s) 252
BfuI GTATCC 1 cut(s) 160
BisI GCNGC 2 cut(s) 551, 853
BlsI GCNGC 2 cut(s) 552, 854
Bme1390I CCNGG 4 cut(s) 417, 631, 678, 921
BmgT120I GGNCC 1 cut(s) 375
BmiI GGNNCC 2 cut(s) 390, 675
BmrFI CCNGG 4 cut(s) 417, 631, 678, 921
BmsI GCATC 4 cut(s) 197, 285, 575, 864
BpiI GAAGAC 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 94
BpuEI CTTGAG 5 cut(s) 298, 332, 575, 627, 842
BpuMI CCSGG 2 cut(s) 417, 631
BsaHI GRCGYC 1 cut(s) 232
BsaI GGTCTC 2 cut(s) 263, 876
BsaJI CCNNGG 2 cut(s) 416, 946
Bsc4I CCNNNNNNNGG 3 cut(s) 874, 875, 952
Bse118I RCCGGY 1 cut(s) 848
BseBI CCWGG 2 cut(s) 678, 921
BseDI CCNNGG 2 cut(s) 416, 946
BseGI GGATG 6 cut(s) 89, 454, 528, 590, 686, 1051
BseLI CCNNNNNNNGG 3 cut(s) 874, 875, 952
BseMII CTCAG 1 cut(s) 108
BseRI GAGGAG 2 cut(s) 285, 584
BseSI GKGCMC 2 cut(s) 393, 563
BseXI GCAGC 2 cut(s) 562, 839
BseYI CCCAGC 3 cut(s) 535, 742, 874
BsgI GTGCAG 1 cut(s) 427
Bsh1236I CGCG 1 cut(s) 437
Bsh1285I CGRYCG 2 cut(s) 903, 1020
BshFI GGCC 2 cut(s) 376, 945
BshNI GGYRCC 1 cut(s) 388
BsiEI CGRYCG 2 cut(s) 903, 1020
BsiHKAI GWGCWC 2 cut(s) 314, 563
BsiSI CCGG 7 cut(s) 364, 398, 416, 481, 630, 849, 942
BslI CCNNNNNNNGG 3 cut(s) 874, 875, 952
BsmAI GTCTC 4 cut(s) 126, 263, 721, 876
BsmBI CGTCTC 1 cut(s) 721
BsmI GAATGC 1 cut(s) 231
BsnI GGCC 2 cut(s) 376, 945
Bso31I GGTCTC 2 cut(s) 263, 876
Bsp1286I GDGCHC 3 cut(s) 314, 393, 563
Bsp143I GATC 4 cut(s) 238, 486, 900, 933
BspACI CCGC 5 cut(s) 386, 437, 450, 879, 1020
BspANI GGCC 2 cut(s) 376, 945
BspCNI CTCAG 1 cut(s) 107
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 2 cut(s) 390, 675
BspPI GGATC 2 cut(s) 233, 481
BspT107I GGYRCC 1 cut(s) 388
BspTNI GGTCTC 2 cut(s) 263, 876
BsrFI RCCGGY 1 cut(s) 848
BssAI RCCGGY 1 cut(s) 848
BssECI CCNNGG 2 cut(s) 416, 946
BssMI GATC 4 cut(s) 238, 486, 900, 933
BssNI GRCGYC 1 cut(s) 232
BssT1I CCWWGG 1 cut(s) 946
Bst2UI CCWGG 2 cut(s) 678, 921
Bst6I CTCTTC 1 cut(s) 787
BstACI GRCGYC 1 cut(s) 232
BstAPI GCANNNNNTGC 2 cut(s) 193, 226
BstC8I GCNNGC 4 cut(s) 218, 374, 446, 450
BstDEI CTNAG 3 cut(s) 94, 357, 565
BstF5I GGATG 6 cut(s) 89, 454, 528, 590, 686, 1051
BstFNI CGCG 1 cut(s) 437
BstHHI GCGC 1 cut(s) 437
BstKTI GATC 4 cut(s) 241, 489, 903, 936
BstMAI GTCTC 4 cut(s) 126, 263, 721, 876
BstMBI GATC 4 cut(s) 238, 486, 900, 933
BstMCI CGRYCG 2 cut(s) 903, 1020
BstMWI GCNNNNNNNGC 8 cut(s) 193, 207, 226, 273, 369, 441, 585, 743
BstNI CCWGG 2 cut(s) 678, 921
BstSCI CCNGG 4 cut(s) 415, 629, 676, 919
BstSLI GKGCMC 2 cut(s) 393, 563
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 2 cut(s) 562, 839
BstV2I GAAGAC 1 cut(s) 282
BstX2I RGATCY 1 cut(s) 486
BstYI RGATCY 1 cut(s) 486
BsuI GTATCC 1 cut(s) 160
BsuRI GGCC 2 cut(s) 376, 945
BtsCI GGATG 6 cut(s) 89, 454, 528, 590, 686, 1051
Cac8I GCNNGC 4 cut(s) 218, 374, 446, 450
CfoI GCGC 1 cut(s) 437
Cfr10I RCCGGY 1 cut(s) 848
Cfr13I GGNCC 1 cut(s) 375
CseI GACGC 1 cut(s) 240
Csp6I GTAC 1 cut(s) 423
CspCI CAANNNNNGTGG 2 cut(s) 651, 686
CviAII CATG 6 cut(s) 76, 184, 188, 502, 952, 1031
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 3 cut(s) 94, 357, 565
DpnI GATC 4 cut(s) 240, 488, 902, 935
DpnII GATC 4 cut(s) 238, 486, 900, 933
EaeI YGGCCR 1 cut(s) 943
Eam1104I CTCTTC 1 cut(s) 787
EarI CTCTTC 1 cut(s) 787
EciI GGCGGA 1 cut(s) 894
Ecl136II GAGCTC 1 cut(s) 312
Eco130I CCWWGG 1 cut(s) 946
Eco24I GRGCYC 1 cut(s) 314
Eco31I GGTCTC 2 cut(s) 263, 876
Eco53kI GAGCTC 1 cut(s) 312
EcoICRI GAGCTC 1 cut(s) 312
EcoRII CCWGG 2 cut(s) 676, 919
EcoT14I CCWWGG 1 cut(s) 946
EcoT22I ATGCAT 2 cut(s) 189, 212
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 946
Esp3I CGTCTC 1 cut(s) 721
FaeI CATG 6 cut(s) 79, 187, 191, 505, 955, 1034
FatI CATG 6 cut(s) 75, 183, 187, 501, 951, 1030
FauI CCCGC 1 cut(s) 457
Fnu4HI GCNGC 2 cut(s) 551, 853
FokI GGATG 6 cut(s) 76, 441, 535, 597, 693, 1058
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 2 cut(s) 551, 853
FspBI CTAG 1 cut(s) 252
GlaI GCGC 1 cut(s) 436
GluI GCNGC 2 cut(s) 551, 853
GsaI CCCAGC 3 cut(s) 539, 746, 878
HaeIII GGCC 2 cut(s) 376, 945
HapII CCGG 7 cut(s) 364, 398, 416, 481, 630, 849, 942
HgaI GACGC 1 cut(s) 240
HhaI GCGC 1 cut(s) 437
Hin1I GRCGYC 1 cut(s) 232
Hin1II CATG 6 cut(s) 79, 187, 191, 505, 955, 1034
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 4 cut(s) 28, 325, 380, 826
HpaII CCGG 7 cut(s) 364, 398, 416, 481, 630, 849, 942
HphI GGTGA 4 cut(s) 173, 330, 838, 1018
Hpy166II GTNNAC 4 cut(s) 121, 271, 423, 561
Hpy188I TCNGA 4 cut(s) 27, 769, 791, 1017
Hpy188III TCNNGA 4 cut(s) 61, 131, 349, 644
Hpy8I GTNNAC 4 cut(s) 121, 271, 423, 561
Hpy99I CGWCG 2 cut(s) 234, 908
HpyAV CCTTC 7 cut(s) 27, 345, 595, 704, 791, 965, 985
HpyCH4IV ACGT 2 cut(s) 285, 998
HpyCH4V TGCA 9 cut(s) 187, 196, 210, 220, 276, 444, 561, 737, 855
HpyF10VI GCNNNNNNNGC 8 cut(s) 193, 207, 226, 273, 369, 441, 585, 743
HpyF3I CTNAG 3 cut(s) 94, 357, 565
HpySE526I ACGT 2 cut(s) 285, 998
Hsp92I GRCGYC 1 cut(s) 232
Hsp92II CATG 6 cut(s) 79, 187, 191, 505, 955, 1034
HspAI GCGC 1 cut(s) 435
Kzo9I GATC 4 cut(s) 238, 486, 900, 933
LmnI GCTCC 3 cut(s) 103, 571, 673
Lsp1109I GCAGC 2 cut(s) 562, 839
LweI GCATC 4 cut(s) 197, 285, 575, 864
MaeI CTAG 1 cut(s) 252
MaeII ACGT 2 cut(s) 285, 998
MaeIII GTNAC 3 cut(s) 604, 658, 994
MalI GATC 4 cut(s) 240, 488, 902, 935
MboI GATC 4 cut(s) 238, 486, 900, 933
MboII GAAGA 7 cut(s) 282, 496, 604, 607, 659, 697, 804
MfeI CAATTG 1 cut(s) 635
MflI RGATCY 1 cut(s) 486
MhlI GDGCHC 3 cut(s) 314, 393, 563
MluCI AATT 3 cut(s) 125, 635, 653
MlyI GAGTC 1 cut(s) 334
MmeI TCCRAC 1 cut(s) 1040
Mph1103I ATGCAT 2 cut(s) 189, 212
MseI TTAA 1 cut(s) 282
MspI CCGG 7 cut(s) 364, 398, 416, 481, 630, 849, 942
MspR9I CCNGG 4 cut(s) 417, 631, 678, 921
MunI CAATTG 1 cut(s) 635
Mva1269I GAATGC 1 cut(s) 231
MvaI CCWGG 2 cut(s) 678, 921
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 8 cut(s) 193, 207, 226, 273, 369, 441, 585, 743
NciI CCSGG 2 cut(s) 417, 631
NdeII GATC 4 cut(s) 238, 486, 900, 933
NlaIII CATG 6 cut(s) 79, 187, 191, 505, 955, 1034
NlaIV GGNNCC 2 cut(s) 390, 675
NmuCI GTSAC 2 cut(s) 604, 994
NsiI ATGCAT 2 cut(s) 189, 212
PctI GAATGC 1 cut(s) 231
PfeI GAWTC 3 cut(s) 28, 380, 826
PflMI CCANNNNNTGG 1 cut(s) 952
PkrI GCNGC 2 cut(s) 552, 854
Ple19I CGATCG 1 cut(s) 903
PleI GAGTC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 333
Psp124BI GAGCTC 1 cut(s) 314
Psp6I CCWGG 2 cut(s) 676, 919
PspFI CCCAGC 3 cut(s) 535, 742, 874
PspGI CCWGG 2 cut(s) 676, 919
PspN4I GGNNCC 2 cut(s) 390, 675
PspPI GGNCC 1 cut(s) 375
PsrI GAACNNNNNNTAC 2 cut(s) 685, 717
PsuI RGATCY 1 cut(s) 486
PvuI CGATCG 1 cut(s) 903
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
SacI GAGCTC 1 cut(s) 314
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 2 cut(s) 551, 853
Sau3AI GATC 4 cut(s) 238, 486, 900, 933
Sau96I GGNCC 1 cut(s) 375
SchI GAGTC 1 cut(s) 334
ScrFI CCNGG 4 cut(s) 417, 631, 678, 921
SduI GDGCHC 3 cut(s) 314, 393, 563
SfaNI GCATC 4 cut(s) 197, 285, 575, 864
SmlI CTYRAG 5 cut(s) 313, 347, 554, 642, 821
SmoI CTYRAG 5 cut(s) 313, 347, 554, 642, 821
Sse9I AATT 3 cut(s) 125, 635, 653
SsiI CCGC 5 cut(s) 386, 437, 450, 879, 1020
SspMI CTAG 1 cut(s) 252
SstI GAGCTC 1 cut(s) 314
StyD4I CCNGG 4 cut(s) 415, 629, 676, 919
StyI CCWWGG 1 cut(s) 946
TaiI ACGT 2 cut(s) 288, 1001
TaqI TCGA 3 cut(s) 130, 903, 1008
TasI AATT 3 cut(s) 125, 635, 653
TatI WGTACW 1 cut(s) 422
TfiI GAWTC 3 cut(s) 28, 380, 826
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TseFI GTSAC 2 cut(s) 604, 994
TseI GCWGC 2 cut(s) 550, 852
Tsp45I GTSAC 2 cut(s) 604, 994
TspDTI ATGAA 7 cut(s) 92, 614, 666, 905, 942, 984, 1047
Van91I CCANNNNNTGG 1 cut(s) 952
VneI GTGCAC 1 cut(s) 559
XspI CTAG 1 cut(s) 252
Zsp2I ATGCAT 2 cut(s) 189, 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.