RLG00000011718

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013997
Physical Location & Seq
Forward (+)
12167733 .. 12169333
1601 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011718

Sequence Viewer

Length: 1137 bp
ATGCGATCCGGTGTAGCCTCCGGAATAGCCTCCATTCAAAATCTCTACAGAGCAGCTTATAGATCCATCACCAGAAAATGGCATAAATATAAGAAGAAACCGGCTTCCGATGAAGAACCCAAAGCTGATGAAGTTCACAAGGTTATAGACAACAATGTACCCGTTGAGGGGGAGGGCAAGGATGGTTTAATCCAAGACGAGAAAAGTATGAATGGTATTCAATATCAAGGCTTTAGATTCAACAGCCCAGGAATGCGAGGAAATGATTCCGGTAGCTCTAGATCTTCGAGCAGCTTCTTCAAGCACTCAAGCGTCGATGGTTTATTTAACATGTCGTGTCATAATCTCACAAGATGTGGTACGCGATCTGCGAGTCACACACCTTCACGTTCTCATCACAAAAAGAAGACTCGGAAAAGCTGTCCGGCCTCACCGGAAACCTCCAACTCATCGGGGAGGAAAAGCTCAACCGACTCAACCAGTACTACGCCTCTCTCAAGAAGTGCACCTCTCTCCAAAAGCGGGCCTCTCTCGAATTGTGAGAGCCGGAGGAGCACCACCATCATGTTTTCGCAGTCATCCGGGATGTTGAAACCCCCGGCCATCGAGAAGCAGCTCGAGTGCACCTTGGAGGAGCTGTGCTTTGGATGCAACAAGAAGATGAAGGTTACGAGGGTTGTTGTCAAAGACACCGGGCAAATGGCACAAGAAGAGGAATTGGTAACGATAAATGTGAAGCCGGGATGGAAGAAAGGAACGAAGATTACATTTGAAGGATTGGGAAATGAGAGACCCGGTGCATACCCAGCTGACATAATTTTTGTGATAGCTGAGAAGAGGCATCATTTGTTTGTAAGAGAAGGAGATGACTTGGAGTTGACAGTAGAAATCCCATTGATACAGTCCCTTACTGGTTGCACAATTTCAATTCCTCTTCTGGGCGGAGAAAATACGACATTGACAATTGAAGACATCATATACCCCGGCTACGAAAAGACCATTCCAGACCAAGGTATGCCGATCTCTAAGGTGGAAGGGAAGAGAGGAAATCTGAAAGTCACGTTCCTGGTTGAGTTCCCTACTTCTCTAACTCACGAGCAAAGATCAGATGTTCAAACTATTTTAGAGGAATGTTAA

Protein Analysis

379

Amino Acids

41.66

Weight (kDa)

8.92

Isoelectric Point (pI)

57.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 202 - 360 1.5e-37 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44160
fragaria_vesca FvH4_2g27360
malus_domestica MD00G1029000.v1.1 MD15G1345600.v1.1
prunus_persica Prupe.1G493700_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0296201
rosa_laevigata RLG00000011718
rosa_multiflora Rmu_sc0028042.1_g000003 Rmu_sc0035469.1_g000001
rosa_roxburghii Rroxscaffold_7G00171750 Rroxscaffold_7G00178310
rosa_rugosa Rorug06G0259400
rosa_samantha Rh6AG371600 Rh6BG379500 Rh6CG384800 Rh6DG372000
rosa_wichuraiana Rw6G032380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 78
AccII CGCG 1 cut(s) 364
AccIII TCCGGA 1 cut(s) 20
AciI CCGC 2 cut(s) 522, 942
AclWI GGATC 1 cut(s) 57
AcoI YGGCCR 1 cut(s) 600
AfaI GTAC 3 cut(s) 159, 361, 484
AfiI CCNNNNNNNGG 6 cut(s) 78, 167, 168, 522, 938, 1010
AflIII ACRYGT 1 cut(s) 330
AgsI TTSAA 9 cut(s) 38, 221, 241, 301, 592, 773, 927, 968, 1115
AjnI CCWGG 2 cut(s) 247, 1065
Alw21I GWGCWC 3 cut(s) 508, 557, 626
Alw26I GTCTC 1 cut(s) 784
Alw44I GTGCAC 2 cut(s) 504, 622
AlwI GGATC 1 cut(s) 57
Ama87I CYCGRG 1 cut(s) 617
Aor13HI TCCGGA 1 cut(s) 20
AoxI GGCC 3 cut(s) 426, 524, 600
ApaLI GTGCAC 2 cut(s) 504, 622
ApeKI GCWGC 3 cut(s) 53, 291, 613
ArsI GACNNNNNNTTYG 2 cut(s) 803, 835
Asp700I GAANNNNTTC 1 cut(s) 265
AspS9I GGNCC 1 cut(s) 524
AsuC2I CCSGG 6 cut(s) 583, 599, 694, 741, 795, 984
AsuHPI GGTGA 2 cut(s) 61, 423
AvaI CYCGRG 1 cut(s) 617
BaeGI GKGCMC 2 cut(s) 508, 626
BauI CACGAG 1 cut(s) 1094
BbsI GAAGAC 2 cut(s) 413, 975
Bbv12I GWGCWC 3 cut(s) 508, 557, 626
BbvI GCAGC 3 cut(s) 65, 303, 625
BccI CCATC 6 cut(s) 74, 176, 311, 569, 611, 738
BciT130I CCWGG 2 cut(s) 249, 1067
BcnI CCSGG 6 cut(s) 583, 599, 694, 741, 795, 984
BcoDI GTCTC 1 cut(s) 784
BfaI CTAG 1 cut(s) 279
BfmI CTRYAG 1 cut(s) 46
BglII AGATCT 1 cut(s) 281
BisI GCNGC 3 cut(s) 54, 292, 614
BlsI GCNGC 3 cut(s) 55, 293, 615
BmcAI AGTACT 1 cut(s) 484
Bme1390I CCNGG 8 cut(s) 249, 583, 599, 694, 741, 795, 984, 1067
BmeT110I CYCGRG 1 cut(s) 617
BmgT120I GGNCC 1 cut(s) 524
BmrFI CCNGG 8 cut(s) 249, 583, 599, 694, 741, 795, 984, 1067
BmsI GCATC 2 cut(s) 638, 850
BpiI GAAGAC 2 cut(s) 413, 975
BpuEI CTTGAG 2 cut(s) 292, 481
BpuMI CCSGG 6 cut(s) 583, 599, 694, 741, 795, 984
BsaI GGTCTC 1 cut(s) 784
BsaJI CCNNGG 5 cut(s) 247, 597, 627, 982, 1009
BsaWI WCCGGW 4 cut(s) 8, 20, 269, 433
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bsc4I CCNNNNNNNGG 6 cut(s) 78, 167, 168, 522, 938, 1010
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 2 cut(s) 480, 916
BseAI TCCGGA 1 cut(s) 20
BseBI CCWGG 2 cut(s) 249, 1067
BseDI CCNNGG 5 cut(s) 247, 597, 627, 982, 1009
BseGI GGATG 5 cut(s) 187, 578, 591, 653, 749
BseLI CCNNNNNNNGG 6 cut(s) 78, 167, 168, 522, 938, 1010
BseMII CTCAG 1 cut(s) 822
BseNI ACTGG 2 cut(s) 480, 916
BseRI GAGGAG 2 cut(s) 565, 647
BseSI GKGCMC 2 cut(s) 508, 626
BseXI GCAGC 3 cut(s) 65, 303, 625
BseYI CCCAGC 1 cut(s) 805
Bsh1236I CGCG 1 cut(s) 364
BshFI GGCC 3 cut(s) 428, 526, 602
BsiHKAI GWGCWC 3 cut(s) 508, 557, 626
BsiHKCI CYCGRG 1 cut(s) 617
BslFI GGGAC 1 cut(s) 889
BslI CCNNNNNNNGG 6 cut(s) 78, 167, 168, 522, 938, 1010
BsmAI GTCTC 1 cut(s) 784
BsmFI GGGAC 1 cut(s) 889
BsmI GAATGC 1 cut(s) 258
BsnI GGCC 3 cut(s) 428, 526, 602
Bso31I GGTCTC 1 cut(s) 784
BsoBI CYCGRG 1 cut(s) 617
Bsp1286I GDGCHC 3 cut(s) 508, 557, 626
Bsp13I TCCGGA 1 cut(s) 20
Bsp143I GATC 6 cut(s) 5, 62, 281, 365, 1020, 1103
BspACI CCGC 2 cut(s) 522, 942
BspANI GGCC 3 cut(s) 428, 526, 602
BspCNI CTCAG 1 cut(s) 823
BspEI TCCGGA 1 cut(s) 20
BspFNI CGCG 1 cut(s) 364
BspPI GGATC 1 cut(s) 57
BspTNI GGTCTC 1 cut(s) 784
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 2 cut(s) 480, 916
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 5 cut(s) 247, 597, 627, 982, 1009
BssMI GATC 6 cut(s) 5, 62, 281, 365, 1020, 1103
BssSI CACGAG 1 cut(s) 1094
BssT1I CCWWGG 2 cut(s) 627, 1009
Bst2BI CACGAG 1 cut(s) 1094
Bst2UI CCWGG 2 cut(s) 249, 1067
Bst4CI ACNGT 2 cut(s) 883, 903
Bst6I CTCTTC 4 cut(s) 705, 830, 939, 1034
BstC8I GCNNGC 1 cut(s) 524
BstDEI CTNAG 2 cut(s) 831, 1026
BstF5I GGATG 5 cut(s) 187, 578, 591, 653, 749
BstFNI CGCG 1 cut(s) 364
BstKTI GATC 6 cut(s) 8, 65, 284, 368, 1023, 1106
BstMAI GTCTC 1 cut(s) 784
BstMBI GATC 6 cut(s) 5, 62, 281, 365, 1020, 1103
BstMWI GCNNNNNNNGC 3 cut(s) 552, 648, 806
BstNI CCWGG 2 cut(s) 249, 1067
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 8 cut(s) 247, 581, 597, 692, 739, 793, 982, 1065
BstSFI CTRYAG 1 cut(s) 46
BstSLI GKGCMC 2 cut(s) 508, 626
BstUI CGCG 1 cut(s) 364
BstV1I GCAGC 3 cut(s) 65, 303, 625
BstV2I GAAGAC 2 cut(s) 413, 975
BstX2I RGATCY 2 cut(s) 62, 281
BstYI RGATCY 2 cut(s) 62, 281
BsuRI GGCC 3 cut(s) 428, 526, 602
BtsCI GGATG 5 cut(s) 187, 578, 591, 653, 749
Cac8I GCNNGC 1 cut(s) 524
Cfr10I RCCGGY 1 cut(s) 100
Cfr13I GGNCC 1 cut(s) 524
CseI GACGC 1 cut(s) 301
Csp6I GTAC 3 cut(s) 158, 360, 483
CviAII CATG 2 cut(s) 331, 565
CviQI GTAC 3 cut(s) 158, 360, 483
DdeI CTNAG 2 cut(s) 831, 1026
DpnI GATC 6 cut(s) 7, 64, 283, 367, 1022, 1105
DpnII GATC 6 cut(s) 5, 62, 281, 365, 1020, 1103
EaeI YGGCCR 1 cut(s) 600
Eam1104I CTCTTC 4 cut(s) 705, 830, 939, 1034
EarI CTCTTC 4 cut(s) 705, 830, 939, 1034
EciI GGCGGA 1 cut(s) 957
Eco130I CCWWGG 2 cut(s) 627, 1009
Eco31I GGTCTC 1 cut(s) 784
Eco88I CYCGRG 1 cut(s) 617
EcoRII CCWGG 2 cut(s) 247, 1065
EcoT14I CCWWGG 2 cut(s) 627, 1009
ErhI CCWWGG 2 cut(s) 627, 1009
FaeI CATG 2 cut(s) 334, 568
FaqI GGGAC 1 cut(s) 889
FatI CATG 2 cut(s) 330, 564
FauI CCCGC 1 cut(s) 515
Fnu4HI GCNGC 3 cut(s) 54, 292, 614
FokI GGATG 5 cut(s) 194, 565, 598, 660, 756
Fsp4HI GCNGC 3 cut(s) 54, 292, 614
FspBI CTAG 1 cut(s) 279
GluI GCNGC 3 cut(s) 54, 292, 614
GsaI CCCAGC 1 cut(s) 809
HaeIII GGCC 3 cut(s) 428, 526, 602
HgaI GACGC 1 cut(s) 301
Hin1II CATG 2 cut(s) 334, 568
HincII GTYRAC 1 cut(s) 879
HindII GTYRAC 1 cut(s) 879
HinfI GANTC 5 cut(s) 237, 266, 373, 409, 473
HphI GGTGA 2 cut(s) 61, 423
Hpy166II GTNNAC 4 cut(s) 136, 506, 624, 879
Hpy188I TCNGA 4 cut(s) 109, 414, 1053, 1108
Hpy188III TCNNGA 7 cut(s) 21, 279, 498, 532, 607, 1004, 1094
Hpy8I GTNNAC 4 cut(s) 136, 506, 624, 879
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 5 cut(s) 393, 658, 767, 854, 1028
HpyCH4III ACNGT 2 cut(s) 883, 903
HpyCH4IV ACGT 2 cut(s) 388, 1061
HpyCH4V TGCA 5 cut(s) 506, 624, 651, 800, 918
HpyF10VI GCNNNNNNNGC 3 cut(s) 552, 648, 806
HpyF3I CTNAG 2 cut(s) 831, 1026
HpySE526I ACGT 2 cut(s) 388, 1061
Hsp92II CATG 2 cut(s) 334, 568
Kpn2I TCCGGA 1 cut(s) 20
Kzo9I GATC 6 cut(s) 5, 62, 281, 365, 1020, 1103
LmnI GCTCC 2 cut(s) 552, 634
Lsp1109I GCAGC 3 cut(s) 65, 303, 625
LweI GCATC 2 cut(s) 638, 850
MaeI CTAG 1 cut(s) 279
MaeII ACGT 2 cut(s) 388, 1061
MaeIII GTNAC 4 cut(s) 374, 667, 721, 1057
MalI GATC 6 cut(s) 7, 64, 283, 367, 1022, 1105
MboI GATC 6 cut(s) 5, 62, 281, 365, 1020, 1103
MfeI CAATTG 1 cut(s) 963
MflI RGATCY 2 cut(s) 62, 281
MhlI GDGCHC 3 cut(s) 508, 557, 626
MluCI AATT 6 cut(s) 535, 716, 816, 921, 927, 963
MlyI GAGTC 3 cut(s) 382, 403, 467
MmeI TCCRAC 1 cut(s) 468
MroI TCCGGA 1 cut(s) 20
MroXI GAANNNNTTC 1 cut(s) 265
MseI TTAA 3 cut(s) 188, 327, 1135
MslI CAYNNNNRTG 1 cut(s) 563
MspA1I CMGCKG 1 cut(s) 809
MspR9I CCNGG 8 cut(s) 249, 583, 599, 694, 741, 795, 984, 1067
MunI CAATTG 1 cut(s) 963
Mva1269I GAATGC 1 cut(s) 258
MvaI CCWGG 2 cut(s) 249, 1067
MvnI CGCG 1 cut(s) 364
MwoI GCNNNNNNNGC 3 cut(s) 552, 648, 806
NciI CCSGG 6 cut(s) 583, 599, 694, 741, 795, 984
NdeII GATC 6 cut(s) 5, 62, 281, 365, 1020, 1103
NlaIII CATG 2 cut(s) 334, 568
NmuCI GTSAC 2 cut(s) 374, 1057
NspI RCATGY 1 cut(s) 334
PaeR7I CTCGAG 1 cut(s) 617
PciI ACATGT 1 cut(s) 330
PcsI WCGNNNNNNNCGW 1 cut(s) 368
PctI GAATGC 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 265
PfeI GAWTC 2 cut(s) 237, 266
PflMI CCANNNNNTGG 1 cut(s) 78
PfoI TCCNGGA 1 cut(s) 581
PkrI GCNGC 3 cut(s) 55, 293, 615
PleI GAGTC 3 cut(s) 381, 403, 467
PpsI GAGTC 3 cut(s) 381, 403, 467
PscI ACATGT 1 cut(s) 330
Psp6I CCWGG 2 cut(s) 247, 1065
PspFI CCCAGC 1 cut(s) 805
PspGI CCWGG 2 cut(s) 247, 1065
PspPI GGNCC 1 cut(s) 524
PspXI VCTCGAGB 1 cut(s) 617
PsrI GAACNNNNNNTAC 2 cut(s) 748, 780
PsuI RGATCY 2 cut(s) 62, 281
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 3 cut(s) 159, 361, 484
RsaNI GTAC 3 cut(s) 158, 360, 483
RseI CAYNNNNRTG 1 cut(s) 563
SaqAI TTAA 3 cut(s) 188, 327, 1135
SatI GCNGC 3 cut(s) 54, 292, 614
Sau3AI GATC 6 cut(s) 5, 62, 281, 365, 1020, 1103
Sau96I GGNCC 1 cut(s) 524
ScaI AGTACT 1 cut(s) 484
SchI GAGTC 3 cut(s) 382, 403, 467
ScrFI CCNGG 8 cut(s) 249, 583, 599, 694, 741, 795, 984, 1067
SduI GDGCHC 3 cut(s) 508, 557, 626
SfaNI GCATC 2 cut(s) 638, 850
SfcI CTRYAG 1 cut(s) 46
Sfr274I CTCGAG 1 cut(s) 617
SlaI CTCGAG 1 cut(s) 617
SmiMI CAYNNNNRTG 1 cut(s) 563
SmlI CTYRAG 3 cut(s) 307, 496, 617
SmoI CTYRAG 3 cut(s) 307, 496, 617
Sse9I AATT 6 cut(s) 535, 716, 816, 921, 927, 963
SsiI CCGC 2 cut(s) 522, 942
SspMI CTAG 1 cut(s) 279
StyD4I CCNGG 8 cut(s) 247, 581, 597, 692, 739, 793, 982, 1065
StyI CCWWGG 2 cut(s) 627, 1009
TaaI ACNGT 2 cut(s) 883, 903
TaiI ACGT 2 cut(s) 391, 1064
TaqI TCGA 5 cut(s) 287, 315, 533, 606, 618
TasI AATT 6 cut(s) 535, 716, 816, 921, 927, 963
TatI WGTACW 1 cut(s) 482
TfiI GAWTC 2 cut(s) 237, 266
Tru1I TTAA 3 cut(s) 188, 327, 1135
Tru9I TTAA 3 cut(s) 188, 327, 1135
TseFI GTSAC 2 cut(s) 374, 1057
TseI GCWGC 3 cut(s) 53, 291, 613
Tsp45I GTSAC 2 cut(s) 374, 1057
TspDTI ATGAA 4 cut(s) 126, 144, 224, 677
Van91I CCANNNNNTGG 1 cut(s) 78
VneI GTGCAC 2 cut(s) 504, 622
XbaI TCTAGA 1 cut(s) 278
XceI RCATGY 1 cut(s) 334
XhoI CTCGAG 1 cut(s) 617
XmnI GAANNNNTTC 1 cut(s) 265
XspI CTAG 1 cut(s) 279
ZrmI AGTACT 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.