MD15G1345600.v1.1

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013997
Physical Location & Seq
Forward (+)
40557503 .. 40559625
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1345600.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGCGCCCTATTACCCCCTCCATTCGGAACCTCTATAGATCTGCCTATAGATCCATTGTCAAGAAATGGCGACACGAGAAAAAAAAATCATCTTCACCTCCAGAAGAGGAAGCCAAAGTCGACCAATTTCGAGACAATGATGTGATTTTTGAGGAAGTGGATGAGGATACAAGACAGGTACAAAAGAAAACCATGCATGGATTTCATAGCTTTAGATGCATCAGCCTGCAAAGAATGCGACGCCATGGTCCATCAAGCCCTAGAGGTCTCAAAAGCGTGGACAGCATCTTTAACGTGTCTTCTCCTCGTTTCTCAAAGAGCTCAAGCCGAAAGAGTCGCACCCCTTCACCCTCCCTCAAGAAAACTAAGAGCCGGAAAAGCTGGCCCGATTCTGTGGCGCCCTCTCTGGCGACCCCTACCACGCCAGGGTGTACCACTAAAAGCGCTGACTGCACGCCCGCCCATCCCTTTCTCTCAAAAAGTGAAAGCCGGAAGAGCCCCACCCCCATCATGTTTTCTAACTCATCTGGGATGTTGAAACCCCCCGCCATCGAGAAGCAGCTTGACTGCACCTTAGAGGAGTTGTGCTTTGGATGCCAGAAGAAGATGAAGGTCACAAGGGCTATCGTTAAAGACACCGGGCAATTGGCTCAAGAAGATGAATTGGTAACAATACAAGTGGAGCCAGGATGGAAGAAAGGAACAAAGATTACATTTCAAGGATTAGGAAATGAGAGACTAGGTGCATACCCAGCAGATATAATCTTTTTGATTTCTGAAAAACGACACCCTTTGTTCAGAAGAGAAGGAGATGACTTGGAATTGGCACTAGAGATTCCATTGGTGGAGGCTCTCACTGGCTGCATCATCTCCATCCCTCTTCTGGGTGGAGACCAGATGAAGTTGACGATCGACGACATCATATATCCTGGCTATGAAAAGATCATATCTGGCCAAGGCATGGCAGACTCTAAAGATGAAGGAAAGAGAGGAAACTTGAAGGTCACGTTTCTGGTTGAGTTTCCGACCGCCCTCACCGATGGGCAACGGTCGGATGTTCTAACCATTTTAGAGGATTCTGATTGA

Protein Analysis

362

Amino Acids

40.37

Weight (kDa)

9.16

Isoelectric Point (pI)

49.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 184 - 342 1.5e-36 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44160
fragaria_vesca FvH4_2g27360
malus_domestica MD00G1029000.v1.1 MD15G1345600.v1.1
prunus_persica Prupe.1G493700_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0296201
rosa_laevigata RLG00000011718
rosa_multiflora Rmu_sc0028042.1_g000003 Rmu_sc0035469.1_g000001
rosa_roxburghii Rroxscaffold_7G00171750 Rroxscaffold_7G00178310
rosa_rugosa Rorug06G0259400
rosa_samantha Rh6AG371600 Rh6BG379500 Rh6CG384800 Rh6DG372000
rosa_wichuraiana Rw6G032380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 246
AccB1I GGYRCC 1 cut(s) 397
AccB7I CCANNNNNTGG 1 cut(s) 961
AccI GTMKAC 1 cut(s) 120
AciI CCGC 3 cut(s) 459, 546, 1029
AclWI GGATC 1 cut(s) 45
AcoI YGGCCR 1 cut(s) 952
AcyI GRCGYC 2 cut(s) 241, 398
AfaI GTAC 2 cut(s) 180, 433
AfeI AGCGCT 1 cut(s) 445
AfiI CCNNNNNNNGG 5 cut(s) 24, 426, 883, 884, 961
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 3 cut(s) 538, 719, 1000
AjnI CCWGG 3 cut(s) 424, 685, 928
AluBI AGCT 4 cut(s) 210, 321, 381, 562
AluI AGCT 4 cut(s) 210, 321, 381, 562
Alw21I GWGCWC 1 cut(s) 323
Alw26I GTCTC 4 cut(s) 126, 272, 730, 885
AlwI GGATC 1 cut(s) 45
Aor51HI AGCGCT 1 cut(s) 445
AoxI GGCC 2 cut(s) 383, 952
ApeKI GCWGC 2 cut(s) 559, 861
AspLEI GCGC 3 cut(s) 6, 400, 446
AspS9I GGNCC 2 cut(s) 248, 384
AsuC2I CCSGG 1 cut(s) 640
AsuHPI GGTGA 3 cut(s) 87, 339, 1027
AvaII GGWCC 1 cut(s) 248
BalI TGGCCA 1 cut(s) 954
BanI GGYRCC 1 cut(s) 397
BanII GRGCYC 2 cut(s) 323, 500
BauI CACGAG 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 323
BbvI GCAGC 2 cut(s) 571, 848
BccI CCATC 7 cut(s) 259, 471, 515, 557, 684, 881, 1034
BciT130I CCWGG 3 cut(s) 426, 687, 930
BciVI GTATCC 1 cut(s) 160
BcnI CCSGG 1 cut(s) 640
BcoDI GTCTC 4 cut(s) 126, 272, 730, 885
BfaI CTAG 3 cut(s) 261, 740, 830
BfmI CTRYAG 2 cut(s) 34, 46
BfoI RGCGCY 2 cut(s) 401, 447
BfuI GTATCC 1 cut(s) 160
BglII AGATCT 1 cut(s) 38
BisI GCNGC 2 cut(s) 560, 862
BlsI GCNGC 2 cut(s) 561, 863
Bme1390I CCNGG 4 cut(s) 426, 640, 687, 930
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 2 cut(s) 248, 384
BmiI GGNNCC 3 cut(s) 29, 399, 684
BmrFI CCNGG 4 cut(s) 426, 640, 687, 930
BmsI GCATC 5 cut(s) 206, 228, 294, 584, 873
BpiI GAAGAC 1 cut(s) 291
BpmI CTGGAG 1 cut(s) 84
BpuEI CTTGAG 3 cut(s) 307, 341, 636
BpuMI CCSGG 1 cut(s) 640
BsaHI GRCGYC 2 cut(s) 241, 398
BsaI GGTCTC 2 cut(s) 272, 885
BsaJI CCNNGG 3 cut(s) 244, 425, 955
Bsc4I CCNNNNNNNGG 5 cut(s) 24, 426, 883, 884, 961
Bse1I ACTGG 1 cut(s) 862
BseBI CCWGG 3 cut(s) 426, 687, 930
BseDI CCNNGG 3 cut(s) 244, 425, 955
BseGI GGATG 7 cut(s) 166, 463, 537, 599, 695, 873, 1060
BseLI CCNNNNNNNGG 5 cut(s) 24, 426, 883, 884, 961
BseNI ACTGG 1 cut(s) 862
BseRI GAGGAG 2 cut(s) 294, 593
BseXI GCAGC 2 cut(s) 571, 848
BseYI CCCAGC 1 cut(s) 751
BsgI GTGCAG 2 cut(s) 436, 553
Bsh1285I CGRYCG 3 cut(s) 912, 1029, 1052
BshFI GGCC 2 cut(s) 385, 954
BshNI GGYRCC 1 cut(s) 397
BsiEI CGRYCG 3 cut(s) 912, 1029, 1052
BsiHKAI GWGCWC 1 cut(s) 323
BsiSI CCGG 3 cut(s) 373, 490, 639
BslI CCNNNNNNNGG 5 cut(s) 24, 426, 883, 884, 961
BsmAI GTCTC 4 cut(s) 126, 272, 730, 885
BsmI GAATGC 1 cut(s) 240
BsnI GGCC 2 cut(s) 385, 954
Bso31I GGTCTC 2 cut(s) 272, 885
Bsp1286I GDGCHC 2 cut(s) 323, 500
Bsp143I GATC 4 cut(s) 38, 50, 909, 942
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 3 cut(s) 459, 546, 1029
BspANI GGCC 2 cut(s) 385, 954
BspLI GGNNCC 3 cut(s) 29, 399, 684
BspPI GGATC 1 cut(s) 45
BspQI GCTCTTC 1 cut(s) 488
BspT107I GGYRCC 1 cut(s) 397
BspTNI GGTCTC 2 cut(s) 272, 885
BsrI ACTGG 1 cut(s) 862
BssECI CCNNGG 3 cut(s) 244, 425, 955
BssMI GATC 4 cut(s) 38, 50, 909, 942
BssNI GRCGYC 2 cut(s) 241, 398
BssSI CACGAG 1 cut(s) 74
BssT1I CCWWGG 2 cut(s) 244, 955
Bst2BI CACGAG 1 cut(s) 74
Bst2UI CCWGG 3 cut(s) 426, 687, 930
Bst4CI ACNGT 1 cut(s) 1050
Bst6I CTCTTC 4 cut(s) 99, 488, 796, 885
BstACI GRCGYC 2 cut(s) 241, 398
BstAPI GCANNNNNTGC 1 cut(s) 235
BstC8I GCNNGC 4 cut(s) 227, 383, 455, 459
BstDEI CTNAG 2 cut(s) 366, 574
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 7 cut(s) 166, 463, 537, 599, 695, 873, 1060
BstH2I RGCGCY 2 cut(s) 401, 447
BstHHI GCGC 3 cut(s) 6, 400, 446
BstKTI GATC 4 cut(s) 41, 53, 912, 945
BstMAI GTCTC 4 cut(s) 126, 272, 730, 885
BstMBI GATC 4 cut(s) 38, 50, 909, 942
BstMCI CGRYCG 3 cut(s) 912, 1029, 1052
BstMWI GCNNNNNNNGC 8 cut(s) 216, 235, 282, 378, 450, 495, 594, 752
BstNI CCWGG 3 cut(s) 426, 687, 930
BstSCI CCNGG 4 cut(s) 424, 638, 685, 928
BstSFI CTRYAG 2 cut(s) 34, 46
BstV1I GCAGC 2 cut(s) 571, 848
BstV2I GAAGAC 1 cut(s) 291
BstX2I RGATCY 2 cut(s) 38, 50
BstYI RGATCY 2 cut(s) 38, 50
BsuI GTATCC 1 cut(s) 160
BsuRI GGCC 2 cut(s) 385, 954
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 7 cut(s) 166, 463, 537, 599, 695, 873, 1060
BtsIMutI CAGTG 1 cut(s) 855
Cac8I GCNNGC 4 cut(s) 227, 383, 455, 459
CfoI GCGC 3 cut(s) 6, 400, 446
Cfr13I GGNCC 2 cut(s) 248, 384
CseI GACGC 1 cut(s) 249
Csp6I GTAC 2 cut(s) 179, 432
CspCI CAANNNNNGTGG 2 cut(s) 660, 695
CviAII CATG 5 cut(s) 193, 197, 245, 511, 961
CviQI GTAC 2 cut(s) 179, 432
DdeI CTNAG 2 cut(s) 366, 574
DinI GGCGCC 1 cut(s) 399
DpnI GATC 4 cut(s) 40, 52, 911, 944
DpnII GATC 4 cut(s) 38, 50, 909, 942
DrdI GACNNNNNNGTC 1 cut(s) 246
DseDI GACNNNNNNGTC 1 cut(s) 246
EaeI YGGCCR 1 cut(s) 952
Eam1104I CTCTTC 4 cut(s) 99, 488, 796, 885
EarI CTCTTC 4 cut(s) 99, 488, 796, 885
Ecl136II GAGCTC 1 cut(s) 321
Eco130I CCWWGG 2 cut(s) 244, 955
Eco24I GRGCYC 2 cut(s) 323, 500
Eco31I GGTCTC 2 cut(s) 272, 885
Eco47I GGWCC 1 cut(s) 248
Eco47III AGCGCT 1 cut(s) 445
Eco53kI GAGCTC 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 321
EcoRII CCWGG 3 cut(s) 424, 685, 928
EcoT14I CCWWGG 2 cut(s) 244, 955
EcoT22I ATGCAT 2 cut(s) 198, 221
EcoT38I GRGCYC 2 cut(s) 323, 500
EgeI GGCGCC 1 cut(s) 399
EheI GGCGCC 1 cut(s) 399
ErhI CCWWGG 2 cut(s) 244, 955
FaeI CATG 5 cut(s) 196, 200, 248, 514, 964
FatI CATG 5 cut(s) 192, 196, 244, 510, 960
FauI CCCGC 2 cut(s) 466, 553
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 2 cut(s) 560, 862
FokI GGATG 7 cut(s) 173, 450, 544, 606, 702, 860, 1067
FriOI GRGCYC 2 cut(s) 323, 500
Fsp4HI GCNGC 2 cut(s) 560, 862
FspBI CTAG 3 cut(s) 261, 740, 830
GlaI GCGC 3 cut(s) 5, 399, 445
GluI GCNGC 2 cut(s) 560, 862
GsaI CCCAGC 1 cut(s) 755
GsuI CTGGAG 1 cut(s) 84
HaeII RGCGCY 2 cut(s) 401, 447
HaeIII GGCC 2 cut(s) 385, 954
HapII CCGG 3 cut(s) 373, 490, 639
HgaI GACGC 1 cut(s) 249
HhaI GCGC 3 cut(s) 6, 400, 446
Hin1I GRCGYC 2 cut(s) 241, 398
Hin1II CATG 5 cut(s) 196, 200, 248, 514, 964
Hin6I GCGC 3 cut(s) 4, 398, 444
HinP1I GCGC 3 cut(s) 4, 398, 444
HincII GTYRAC 2 cut(s) 121, 906
HindII GTYRAC 2 cut(s) 121, 906
HinfI GANTC 5 cut(s) 334, 389, 835, 968, 1076
HpaII CCGG 3 cut(s) 373, 490, 639
HphI GGTGA 3 cut(s) 87, 339, 1027
Hpy166II GTNNAC 4 cut(s) 121, 280, 432, 906
Hpy188I TCNGA 6 cut(s) 27, 778, 800, 1026, 1054, 1081
Hpy188III TCNNGA 6 cut(s) 61, 101, 131, 358, 553, 653
Hpy8I GTNNAC 4 cut(s) 121, 280, 432, 906
Hpy99I CGWCG 2 cut(s) 243, 917
HpyAV CCTTC 5 cut(s) 354, 604, 800, 974, 994
HpyCH4III ACNGT 1 cut(s) 1050
HpyCH4IV ACGT 2 cut(s) 294, 1007
HpyCH4V TGCA 7 cut(s) 196, 219, 229, 453, 570, 746, 864
HpyF10VI GCNNNNNNNGC 8 cut(s) 216, 235, 282, 378, 450, 495, 594, 752
HpyF3I CTNAG 2 cut(s) 366, 574
HpySE526I ACGT 2 cut(s) 294, 1007
Hsp92I GRCGYC 2 cut(s) 241, 398
Hsp92II CATG 5 cut(s) 196, 200, 248, 514, 964
HspAI GCGC 3 cut(s) 4, 398, 444
KasI GGCGCC 1 cut(s) 397
Kzo9I GATC 4 cut(s) 38, 50, 909, 942
LguI GCTCTTC 1 cut(s) 488
LmnI GCTCC 1 cut(s) 682
Lsp1109I GCAGC 2 cut(s) 571, 848
LweI GCATC 5 cut(s) 206, 228, 294, 584, 873
MaeI CTAG 3 cut(s) 261, 740, 830
MaeII ACGT 2 cut(s) 294, 1007
MaeIII GTNAC 3 cut(s) 613, 667, 1003
MalI GATC 4 cut(s) 40, 52, 911, 944
MboI GATC 4 cut(s) 38, 50, 909, 942
MfeI CAATTG 1 cut(s) 644
MflI RGATCY 2 cut(s) 38, 50
MhlI GDGCHC 2 cut(s) 323, 500
MlsI TGGCCA 1 cut(s) 954
MluCI AATT 4 cut(s) 125, 644, 662, 821
MluNI TGGCCA 1 cut(s) 954
Mly113I GGCGCC 1 cut(s) 398
MlyI GAGTC 2 cut(s) 343, 962
MmeI TCCRAC 2 cut(s) 1032, 1049
Mox20I TGGCCA 1 cut(s) 954
Mph1103I ATGCAT 2 cut(s) 198, 221
MscI TGGCCA 1 cut(s) 954
MseI TTAA 2 cut(s) 291, 630
Msp20I TGGCCA 1 cut(s) 954
MspI CCGG 3 cut(s) 373, 490, 639
MspR9I CCNGG 4 cut(s) 426, 640, 687, 930
MunI CAATTG 1 cut(s) 644
Mva1269I GAATGC 1 cut(s) 240
MvaI CCWGG 3 cut(s) 426, 687, 930
MwoI GCNNNNNNNGC 8 cut(s) 216, 235, 282, 378, 450, 495, 594, 752
NarI GGCGCC 1 cut(s) 398
NciI CCSGG 1 cut(s) 640
NcoI CCATGG 1 cut(s) 244
NdeII GATC 4 cut(s) 38, 50, 909, 942
NlaIII CATG 5 cut(s) 196, 200, 248, 514, 964
NlaIV GGNNCC 3 cut(s) 29, 399, 684
NmuCI GTSAC 2 cut(s) 613, 1003
NsiI ATGCAT 2 cut(s) 198, 221
PciSI GCTCTTC 1 cut(s) 488
PctI GAATGC 1 cut(s) 240
PfeI GAWTC 3 cut(s) 389, 835, 1076
PflMI CCANNNNNTGG 1 cut(s) 961
PkrI GCNGC 2 cut(s) 561, 863
Ple19I CGATCG 1 cut(s) 912
PleI GAGTC 2 cut(s) 342, 962
PluTI GGCGCC 1 cut(s) 401
PpsI GAGTC 2 cut(s) 342, 962
Psp124BI GAGCTC 1 cut(s) 323
Psp6I CCWGG 3 cut(s) 424, 685, 928
PspFI CCCAGC 1 cut(s) 751
PspGI CCWGG 3 cut(s) 424, 685, 928
PspN4I GGNNCC 3 cut(s) 29, 399, 684
PspPI GGNCC 2 cut(s) 248, 384
PsrI GAACNNNNNNTAC 2 cut(s) 694, 726
PsuI RGATCY 2 cut(s) 38, 50
PvuI CGATCG 1 cut(s) 912
RsaI GTAC 2 cut(s) 180, 433
RsaNI GTAC 2 cut(s) 179, 432
SacI GAGCTC 1 cut(s) 323
SalI GTCGAC 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 488
SaqAI TTAA 2 cut(s) 291, 630
SatI GCNGC 2 cut(s) 560, 862
Sau3AI GATC 4 cut(s) 38, 50, 909, 942
Sau96I GGNCC 2 cut(s) 248, 384
SchI GAGTC 2 cut(s) 343, 962
ScrFI CCNGG 4 cut(s) 426, 640, 687, 930
SduI GDGCHC 2 cut(s) 323, 500
SfaNI GCATC 5 cut(s) 206, 228, 294, 584, 873
SfcI CTRYAG 2 cut(s) 34, 46
SfoI GGCGCC 1 cut(s) 399
SinI GGWCC 1 cut(s) 248
SmlI CTYRAG 3 cut(s) 322, 356, 651
SmoI CTYRAG 3 cut(s) 322, 356, 651
Sse9I AATT 4 cut(s) 125, 644, 662, 821
SsiI CCGC 3 cut(s) 459, 546, 1029
SspDI GGCGCC 1 cut(s) 397
SspMI CTAG 3 cut(s) 261, 740, 830
SstI GAGCTC 1 cut(s) 323
StyD4I CCNGG 4 cut(s) 424, 638, 685, 928
StyI CCWWGG 2 cut(s) 244, 955
TaaI ACNGT 1 cut(s) 1050
TaiI ACGT 2 cut(s) 297, 1010
TaqI TCGA 4 cut(s) 120, 130, 552, 912
TasI AATT 4 cut(s) 125, 644, 662, 821
TfiI GAWTC 3 cut(s) 389, 835, 1076
Tru1I TTAA 2 cut(s) 291, 630
Tru9I TTAA 2 cut(s) 291, 630
TscAI CASTG 1 cut(s) 862
TseFI GTSAC 2 cut(s) 613, 1003
TseI GCWGC 2 cut(s) 559, 861
Tsp45I GTSAC 2 cut(s) 613, 1003
TspDTI ATGAA 6 cut(s) 194, 623, 675, 914, 951, 993
TspRI CASTG 1 cut(s) 862
Van91I CCANNNNNTGG 1 cut(s) 961
VpaK11BI GGWCC 1 cut(s) 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 880
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 3 cut(s) 261, 740, 830
Zsp2I ATGCAT 2 cut(s) 198, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.