FvH4_2g28020

methyltransferase PMT10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011820
Physical Location & Seq
Forward (+)
22113344 .. 22118186
4843 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28020.t1

Sequence Viewer

Length: 1962 bp
ATGAAGCTGGCCGCGACGGCGGCGGAGATCTTGAAGACTCCGACCTTCATCAAAATAACGGCTATTGCCATCGCCTCCCTCACCCTCTTCATCCTCGTCAACCACTACTCCGCCTCCTACTCTCCCCTCCCCTCCTCCGCCGCGACGACTCACTCTCTCGCAGCTTCTCCGCCGACGGAATCATCCTCTCGGCCGGCGTCTCCTCCTCTTCCTGCGCCAACACCGCCTCCACCTCCGCCGCCGCCGGAGGTGGAGAGGATGGGGATAGTGGACGAGAACGGCGCCATGTCGGAGGAATTCACGGTCGGCGAGTTCGACGAGAGCTTGGCCGAGGAGCTGAGGAATGCGGGCGGAGGAGGAGCCGAGGATGGGAGTGGCGGCAATGGCGGTGGCGGTAGGGTTAAGGTTGACAAGTTTGGGGTGTGTGATCAGAAATTGACTGATTATATACCGTGTTTGGATAATGTGGAGGAGATTAAGAAGCTCAATTCCACTGAGAGAGGCGAAAAGTTCGAAAGGCATTGTCCTGGAGAAGGCAAGAGGTTGAATTGCGTGGTGCCGAGGCCGAAAGGGTATCAGATTCGAATTCCCTGGCCTCAGAGCAGAGATGAGGTGTGGTTCAGTAATGTACCCCATAAAAGACTAGTTGAAGATAAAGGTGGTCAGAATTGGATAGCAATAAAGGGAGATAAGTTTGTGTTTCCTGGAGGTGGAACACAGTTCATTCATGGGGCAGATGAGTATTTGGATCAGATTTCTAAGATGATTCCTGAAATTGCATTTGGCCGCAACACCCGAGTAGCCTTAGATGTTGGTTGTGGAGTAGCGAGTTTTGGTGCCTTTTTGATGCAACGGAATGTGACCACTCTGTCAGTTGCGCCGAAAGATGTCCATGAGAACCAGATCCAGTTTGCACTTGAGCGTGGTGTGCCTGCTATGGTGGCAGTATTTGCTACTCACCGCTTGTTGTATCCAAGCCAGGCTTTTGACTTGATCCACTGTTCAAGATGTAGAATTAATTGGACTCGCGATGATGGAATTTGGCTTCTCGAGGTTGACAGGTTGCTGAGAGCAGGAGGATATTTTGTTTGGGCAGCGCAGCCTGTTTATAAACATGAAGTAGCCCTACAAGAACAATGGAAAGAAATGGAGGACCTGACTACGCGCATTTGTTGGGAACTTGTAAAGAAGGAAGGGATGATTGCCATATGGAGGAAACCTTTAAATAACAGCTGCTATCTTAGCCGTGATGCTGGAGTGCAGCCTCCATTGTGTGATTCCAATGACGATCCAGACAATGTTTGGTATGTTGGTTTGAAGGCATGCATCAGTCGGTTACCTGAAAATGGTTATGGAGCAAATGTTTCAATGTGGCCTGCACGGGTTCATGATCCACCAGACAGACTCCAGAGCATAAAATTAGATGCCTACATATCTAGAAAAGAAATCTTCAGGGCAGAGGCGAAGTACTGGAACGAAATAATTAGCGCTTATATTGGTGCTTACCATTGGAAAGAGTTAAACTTCAGAAACATAATGGACATGAGGGCTGGATATGGAGGGTTTGCAGCAGCATTGCATGACCTCGGGCTAGATTGTTGGGTAATGAACGTTGTTCCTGTGTCTGGGTTCAACACCTTACCGGTTATTTATGACCGTGGACTTATTGGAGTCCAGCATGACTGGTGTGAGCCATTTGACACTTATCCAAGAACATATGACTTATTGCACGCAGCAGGTCTCTTTTCTATTGAGCAGAAGAGGTGTAATTTCTCAACCATCATTCTTGAAATGGACCGAATGTTAAGGCCTGGGGGACGGGTTTATATACGCGACTCTGTGTCTGTACTCGGTGAGATTGAAGAACTTGCAAGTGCAGTTGGATGGGTGCCTGCACTACATGATACAGGAGAAGGACCCCATGCAAGCTGGAAGATCTTAATAAGTGACAAGCGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

654

Amino Acids

72.66

Weight (kDa)

5.8

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 148 - 651 8.7e-227 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 269 - 363 4.6e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77260
fragaria_vesca FvH4_2g28020
malus_domestica MD15G1132900.v1.1
prunus_persica Prupe.1G488500_v2.0.a1 Prupe.1G488500_v2.0.a1
pyrus_communis pycom15g11980
rosa_chinensis RchiOBHm_Chr6g0296881
rosa_laevigata RLG00000011649
rosa_multiflora Rmu_co8446841.1_g000001 Rmu_sc0033097.1_g000001 Rmu_sc0038066.1_g000001
rosa_roxburghii Rroxscaffold_7G00171060
rosa_rugosa Rorug06G0267200 Rorug06G0267300
rosa_samantha Rh6AG378000 Rh6BG386000 Rh6CG391400 Rh6DG378700
rosa_wichuraiana Rw6G033040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1110
AasI GACNNNNNNGTC 1 cut(s) 868
Acc36I ACCTGC 1 cut(s) 1727
AccB1I GGYRCC 4 cut(s) 281, 556, 836, 1888
AccII CGCG 5 cut(s) 14, 143, 1029, 1165, 1833
AclI AACGTT 1 cut(s) 1611
AclWI GGATC 5 cut(s) 756, 898, 988, 1283, 1385
AcoI YGGCCR 4 cut(s) 9, 191, 327, 784
AcsI RAATTY 3 cut(s) 296, 585, 1038
AcuI CTGAAG 2 cut(s) 1435, 1510
AcyI GRCGYC 2 cut(s) 197, 282
AfaI GTAC 3 cut(s) 630, 1469, 1848
AfeI AGCGCT 1 cut(s) 1489
AfiI CCNNNNNNNGG 6 cut(s) 369, 533, 710, 1212, 1346, 1625
AgeI ACCGGT 1 cut(s) 1642
AgsI TTSAA 9 cut(s) 34, 547, 650, 1005, 1318, 1368, 1633, 1790, 1862
AhdI GACNNNNNGTC 1 cut(s) 1840
AhlI ACTAGT 1 cut(s) 643
AjnI CCWGG 5 cut(s) 526, 590, 703, 978, 1810
AjuI GAANNNNNNNTTGG 2 cut(s) 765, 797
AluBI AGCT 7 cut(s) 7, 164, 324, 337, 484, 1233, 1929
AluI AGCT 7 cut(s) 7, 164, 324, 337, 484, 1233, 1929
Alw26I GTCTC 2 cut(s) 204, 1745
AlwI GGATC 5 cut(s) 756, 898, 988, 1283, 1385
Ama87I CYCGRG 3 cut(s) 795, 1049, 1586
Aor51HI AGCGCT 1 cut(s) 1489
AoxI GGCC 8 cut(s) 9, 191, 327, 563, 593, 784, 1373, 1808
ApeKI GCWGC 8 cut(s) 161, 1094, 1099, 1233, 1261, 1568, 1571, 1733
ApoI RAATTY 3 cut(s) 296, 585, 1038
AseI ATTAAT 1 cut(s) 1017
AsiGI ACCGGT 1 cut(s) 1642
AspLEI GCGC 6 cut(s) 217, 284, 880, 1099, 1167, 1490
AspS9I GGNCC 3 cut(s) 1153, 1795, 1916
AsuHPI GGTGA 3 cut(s) 73, 950, 1865
AsuII TTCGAA 2 cut(s) 513, 583
AvaI CYCGRG 3 cut(s) 795, 1049, 1586
AvaII GGWCC 3 cut(s) 1153, 1795, 1916
BanI GGYRCC 4 cut(s) 281, 556, 836, 1888
BarI GAAGNNNNNNTAC 2 cut(s) 1110, 1142
BbsI GAAGAC 1 cut(s) 41
BbvCI CCTCAGC 1 cut(s) 338
BbvI GCAGC 8 cut(s) 173, 1106, 1111, 1220, 1273, 1580, 1583, 1745
BccI CCATC 6 cut(s) 77, 253, 362, 1028, 1787, 1878
BceAI ACGGC 4 cut(s) 33, 75, 295, 1230
BciT130I CCWGG 5 cut(s) 528, 592, 705, 980, 1812
BciVI GTATCC 1 cut(s) 981
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 2 cut(s) 204, 1745
BcuI ACTAGT 1 cut(s) 643
BfaI CTAG 3 cut(s) 644, 1437, 1592
BfoI RGCGCY 2 cut(s) 285, 1491
BfuAI ACCTGC 1 cut(s) 1727
BfuI GTATCC 1 cut(s) 981
BglI GCCNNNNNGGC 1 cut(s) 17
BglII AGATCT 2 cut(s) 27, 1935
BmcAI AGTACT 1 cut(s) 1469
Bme1390I CCNGG 5 cut(s) 528, 592, 705, 980, 1812
Bme18I GGWCC 3 cut(s) 1153, 1795, 1916
BmeRI GACNNNNNGTC 1 cut(s) 1840
BmeT110I CYCGRG 3 cut(s) 795, 1049, 1586
BmgT120I GGNCC 3 cut(s) 1153, 1795, 1916
BmiI GGNNCC 6 cut(s) 283, 361, 558, 838, 1890, 1918
BmrFI CCNGG 5 cut(s) 528, 592, 705, 980, 1812
BmsI GCATC 4 cut(s) 837, 1240, 1335, 1414
BpiI GAAGAC 1 cut(s) 41
BpmI CTGGAG 4 cut(s) 549, 726, 1275, 1391
Bpu10I CCTNAGC 1 cut(s) 338
Bpu14I TTCGAA 2 cut(s) 513, 583
BpuEI CTTGAG 1 cut(s) 938
BsaHI GRCGYC 2 cut(s) 197, 282
BsaI GGTCTC 1 cut(s) 1745
BsaJI CCNNGG 7 cut(s) 330, 363, 560, 590, 1585, 1657, 1811
BsaWI WCCGGW 1 cut(s) 1642
BsaXI ACNNNNNCTCC 8 cut(s) 92, 98, 122, 128, 211, 241, 1347, 1377
Bsc4I CCNNNNNNNGG 6 cut(s) 369, 533, 710, 1212, 1346, 1625
Bse118I RCCGGY 2 cut(s) 193, 1642
Bse1I ACTGG 3 cut(s) 907, 1475, 1688
Bse3DI GCAATG 2 cut(s) 388, 1574
BseBI CCWGG 5 cut(s) 528, 592, 705, 980, 1812
BseDI CCNNGG 7 cut(s) 330, 363, 560, 590, 1585, 1657, 1811
BseGI GGATG 6 cut(s) 90, 182, 264, 373, 1203, 1889
BseLI CCNNNNNNNGG 6 cut(s) 369, 533, 710, 1212, 1346, 1625
BseMI GCAATG 2 cut(s) 388, 1574
BseMII CTCAG 4 cut(s) 329, 486, 611, 1058
BseNI ACTGG 3 cut(s) 907, 1475, 1688
BseRI GAGGAG 7 cut(s) 124, 192, 195, 347, 369, 372, 485
BseX3I CGGCCG 1 cut(s) 191
BseXI GCAGC 8 cut(s) 173, 1106, 1111, 1220, 1273, 1580, 1583, 1745
BsgI GTGCAG 4 cut(s) 1280, 1362, 1878, 1896
Bsh1236I CGCG 5 cut(s) 14, 143, 1029, 1165, 1833
Bsh1285I CGRYCG 2 cut(s) 194, 306
BshFI GGCC 8 cut(s) 11, 193, 329, 565, 595, 786, 1375, 1810
BshNI GGYRCC 4 cut(s) 281, 556, 836, 1888
BshTI ACCGGT 1 cut(s) 1642
BsiEI CGRYCG 2 cut(s) 194, 306
BsiHKCI CYCGRG 3 cut(s) 795, 1049, 1586
BsiSI CCGG 3 cut(s) 194, 245, 1643
BslFI GGGAC 1 cut(s) 1830
BslI CCNNNNNNNGG 6 cut(s) 369, 533, 710, 1212, 1346, 1625
BsmAI GTCTC 2 cut(s) 204, 1745
BsmBI CGTCTC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 1830
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 8 cut(s) 11, 193, 329, 565, 595, 786, 1375, 1810
Bso31I GGTCTC 1 cut(s) 1745
BsoBI CYCGRG 3 cut(s) 795, 1049, 1586
Bsp119I TTCGAA 2 cut(s) 513, 583
Bsp143I GATC 8 cut(s) 27, 427, 748, 903, 993, 1288, 1390, 1935
Bsp68I TCGCGA 1 cut(s) 1029
BspANI GGCC 8 cut(s) 11, 193, 329, 565, 595, 786, 1375, 1810
BspCNI CTCAG 4 cut(s) 330, 487, 610, 1059
BspFNI CGCG 5 cut(s) 14, 143, 1029, 1165, 1833
BspHI TCATGA 1 cut(s) 1387
BspLI GGNNCC 6 cut(s) 283, 361, 558, 838, 1890, 1918
BspMI ACCTGC 1 cut(s) 1727
BspPI GGATC 5 cut(s) 756, 898, 988, 1283, 1385
BspT104I TTCGAA 2 cut(s) 513, 583
BspT107I GGYRCC 4 cut(s) 281, 556, 836, 1888
BspTNI GGTCTC 1 cut(s) 1745
BsrDI GCAATG 2 cut(s) 388, 1574
BsrFI RCCGGY 2 cut(s) 193, 1642
BsrI ACTGG 3 cut(s) 907, 1475, 1688
BssAI RCCGGY 2 cut(s) 193, 1642
BssECI CCNNGG 7 cut(s) 330, 363, 560, 590, 1585, 1657, 1811
BssMI GATC 8 cut(s) 27, 427, 748, 903, 993, 1288, 1390, 1935
BssNI GRCGYC 2 cut(s) 197, 282
Bst2UI CCWGG 5 cut(s) 528, 592, 705, 980, 1812
Bst4CI ACNGT 5 cut(s) 304, 453, 720, 1001, 1658
Bst6I CTCTTC 3 cut(s) 92, 213, 1754
BstACI GRCGYC 2 cut(s) 197, 282
BstAPI GCANNNNNTGC 1 cut(s) 950
BstBI TTCGAA 2 cut(s) 513, 583
BstC8I GCNNGC 9 cut(s) 9, 195, 349, 933, 1324, 1377, 1731, 1893, 1927
BstDEI CTNAG 7 cut(s) 338, 495, 597, 759, 805, 1067, 1241
BstDSI CCRYGG 1 cut(s) 1657
BstEII GGTNACC 1 cut(s) 1335
BstENI CCTNNNNNAGG 1 cut(s) 531
BstF5I GGATG 6 cut(s) 90, 182, 264, 373, 1203, 1889
BstFNI CGCG 5 cut(s) 14, 143, 1029, 1165, 1833
BstH2I RGCGCY 2 cut(s) 285, 1491
BstHHI GCGC 6 cut(s) 217, 284, 880, 1099, 1167, 1490
BstKTI GATC 8 cut(s) 30, 430, 751, 906, 996, 1291, 1393, 1938
BstMAI GTCTC 2 cut(s) 204, 1745
BstMBI GATC 8 cut(s) 27, 427, 748, 903, 993, 1288, 1390, 1935
BstMCI CGRYCG 2 cut(s) 194, 306
BstMWI GCNNNNNNNGC 8 cut(s) 17, 20, 223, 384, 928, 941, 950, 1242
BstNI CCWGG 5 cut(s) 528, 592, 705, 980, 1812
BstNSI RCATGY 1 cut(s) 1326
BstPI GGTNACC 1 cut(s) 1335
BstSCI CCNGG 5 cut(s) 526, 590, 703, 978, 1810
BstUI CGCG 5 cut(s) 14, 143, 1029, 1165, 1833
BstV1I GCAGC 8 cut(s) 173, 1106, 1111, 1220, 1273, 1580, 1583, 1745
BstV2I GAAGAC 1 cut(s) 41
BstX2I RGATCY 3 cut(s) 27, 903, 1935
BstYI RGATCY 3 cut(s) 27, 903, 1935
BstZI CGGCCG 1 cut(s) 191
BsuI GTATCC 1 cut(s) 981
BsuRI GGCC 8 cut(s) 11, 193, 329, 565, 595, 786, 1375, 1810
BtgI CCRYGG 1 cut(s) 1657
BtgZI GCGATG 2 cut(s) 55, 1044
BtsCI GGATG 6 cut(s) 90, 182, 264, 373, 1203, 1889
BtsIMutI CAGTG 2 cut(s) 492, 997
BtuMI TCGCGA 1 cut(s) 1029
BveI ACCTGC 1 cut(s) 1727
Cac8I GCNNGC 9 cut(s) 9, 195, 349, 933, 1324, 1377, 1731, 1893, 1927
CciI TCATGA 1 cut(s) 1387
CfoI GCGC 6 cut(s) 217, 284, 880, 1099, 1167, 1490
Cfr10I RCCGGY 2 cut(s) 193, 1642
Cfr13I GGNCC 3 cut(s) 1153, 1795, 1916
CseI GACGC 1 cut(s) 186
Csp6I GTAC 3 cut(s) 629, 1468, 1847
CspAI ACCGGT 1 cut(s) 1642
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviQI GTAC 3 cut(s) 629, 1468, 1847
DdeI CTNAG 7 cut(s) 338, 495, 597, 759, 805, 1067, 1241
DinI GGCGCC 1 cut(s) 283
DpnI GATC 8 cut(s) 29, 429, 750, 905, 995, 1290, 1392, 1937
DpnII GATC 8 cut(s) 27, 427, 748, 903, 993, 1288, 1390, 1935
DraI TTTAAA 1 cut(s) 1224
DrdI GACNNNNNNGTC 1 cut(s) 868
DriI GACNNNNNGTC 1 cut(s) 1840
DseDI GACNNNNNNGTC 1 cut(s) 868
EaeI YGGCCR 4 cut(s) 9, 191, 327, 784
EagI CGGCCG 1 cut(s) 191
Eam1104I CTCTTC 3 cut(s) 92, 213, 1754
Eam1105I GACNNNNNGTC 1 cut(s) 1840
EarI CTCTTC 3 cut(s) 92, 213, 1754
EciI GGCGGA 6 cut(s) 38, 100, 127, 159, 225, 366
EclXI CGGCCG 1 cut(s) 191
Eco147I AGGCCT 1 cut(s) 1810
Eco31I GGTCTC 1 cut(s) 1745
Eco47I GGWCC 3 cut(s) 1153, 1795, 1916
Eco47III AGCGCT 1 cut(s) 1489
Eco52I CGGCCG 1 cut(s) 191
Eco57I CTGAAG 2 cut(s) 1435, 1510
Eco88I CYCGRG 3 cut(s) 795, 1049, 1586
Eco91I GGTNACC 1 cut(s) 1335
EcoNI CCTNNNNNAGG 1 cut(s) 531
EcoO109I RGGNCCY 2 cut(s) 1153, 1916
EcoO65I GGTNACC 1 cut(s) 1335
EcoRI GAATTC 2 cut(s) 296, 585
EcoRII CCWGG 5 cut(s) 526, 590, 703, 978, 1810
EcoT22I ATGCAT 1 cut(s) 1328
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
Esp3I CGTCTC 1 cut(s) 204
FalI AAGNNNNNCTT 2 cut(s) 967, 999
FaqI GGGAC 1 cut(s) 1830
FauI CCCGC 1 cut(s) 340
FauNDI CATATG 2 cut(s) 1208, 1717
FbaI TGATCA 1 cut(s) 427
FokI GGATG 6 cut(s) 77, 169, 271, 380, 1210, 1896
FspBI CTAG 3 cut(s) 644, 1437, 1592
GlaI GCGC 6 cut(s) 216, 283, 879, 1098, 1166, 1489
GsuI CTGGAG 4 cut(s) 549, 726, 1275, 1391
HaeII RGCGCY 2 cut(s) 285, 1491
HaeIII GGCC 8 cut(s) 11, 193, 329, 565, 595, 786, 1375, 1810
HapII CCGG 3 cut(s) 194, 245, 1643
HgaI GACGC 1 cut(s) 186
HhaI GCGC 6 cut(s) 217, 284, 880, 1099, 1167, 1490
Hin1I GRCGYC 2 cut(s) 197, 282
Hin6I GCGC 6 cut(s) 215, 282, 878, 1097, 1165, 1488
HinP1I GCGC 6 cut(s) 215, 282, 878, 1097, 1165, 1488
HincII GTYRAC 3 cut(s) 100, 409, 1057
HindII GTYRAC 3 cut(s) 100, 409, 1057
HpaII CCGG 3 cut(s) 194, 245, 1643
HphI GGTGA 3 cut(s) 73, 950, 1865
Hpy166II GTNNAC 5 cut(s) 100, 271, 409, 1057, 1661
Hpy188I TCNGA 8 cut(s) 42, 292, 432, 579, 600, 666, 753, 1529
Hpy8I GTNNAC 5 cut(s) 100, 271, 409, 1057, 1661
Hpy99I CGWCG 4 cut(s) 19, 148, 178, 320
HpyAV CCTTC 6 cut(s) 55, 527, 1183, 1187, 1312, 1907
HpyCH4III ACNGT 5 cut(s) 304, 453, 720, 1001, 1658
HpyCH4IV ACGT 1 cut(s) 1611
HpyF10VI GCNNNNNNNGC 8 cut(s) 17, 20, 223, 384, 928, 941, 950, 1242
HpyF3I CTNAG 7 cut(s) 338, 495, 597, 759, 805, 1067, 1241
HpySE526I ACGT 1 cut(s) 1611
Hsp92I GRCGYC 2 cut(s) 197, 282
HspAI GCGC 6 cut(s) 215, 282, 878, 1097, 1165, 1488
KasI GGCGCC 1 cut(s) 281
KroI GCCGGC 1 cut(s) 193
KroNI GCCGGC 1 cut(s) 195
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 8 cut(s) 27, 427, 748, 903, 993, 1288, 1390, 1935
LmnI GCTCC 3 cut(s) 334, 359, 1355
Lsp1109I GCAGC 8 cut(s) 173, 1106, 1111, 1220, 1273, 1580, 1583, 1745
LweI GCATC 4 cut(s) 837, 1240, 1335, 1414
MaeI CTAG 3 cut(s) 644, 1437, 1592
MaeII ACGT 1 cut(s) 1611
MaeIII GTNAC 3 cut(s) 859, 1335, 1946
MalI GATC 8 cut(s) 29, 429, 750, 905, 995, 1290, 1392, 1937
MboI GATC 8 cut(s) 27, 427, 748, 903, 993, 1288, 1390, 1935
MboII GAAGA 8 cut(s) 46, 79, 200, 662, 1441, 1771, 1874, 1945
MflI RGATCY 3 cut(s) 27, 903, 1935
Mly113I GGCGCC 1 cut(s) 282
MlyI GAGTC 6 cut(s) 31, 142, 1018, 1398, 1680, 1829
MmeI TCCRAC 3 cut(s) 65, 270, 1861
Mph1103I ATGCAT 1 cut(s) 1328
MroNI GCCGGC 1 cut(s) 193
MseI TTAA 7 cut(s) 402, 477, 1017, 1223, 1520, 1805, 1940
MspA1I CMGCKG 1 cut(s) 1233
MspI CCGG 3 cut(s) 194, 245, 1643
MspR9I CCNGG 5 cut(s) 528, 592, 705, 980, 1812
Mva1269I GAATGC 1 cut(s) 349
MvaI CCWGG 5 cut(s) 528, 592, 705, 980, 1812
MvnI CGCG 5 cut(s) 14, 143, 1029, 1165, 1833
MwoI GCNNNNNNNGC 8 cut(s) 17, 20, 223, 384, 928, 941, 950, 1242
NaeI GCCGGC 1 cut(s) 195
NarI GGCGCC 1 cut(s) 282
NdeI CATATG 2 cut(s) 1208, 1717
NdeII GATC 8 cut(s) 27, 427, 748, 903, 993, 1288, 1390, 1935
NgoMIV GCCGGC 1 cut(s) 193
NlaIV GGNNCC 6 cut(s) 283, 361, 558, 838, 1890, 1918
NmeAIII GCCGAG 4 cut(s) 169, 355, 388, 585
NmuCI GTSAC 2 cut(s) 859, 1946
NruI TCGCGA 1 cut(s) 1029
NsiI ATGCAT 1 cut(s) 1328
NspI RCATGY 1 cut(s) 1326
NspV TTCGAA 2 cut(s) 513, 583
PaeI GCATGC 1 cut(s) 1326
PaeR7I CTCGAG 1 cut(s) 1049
PagI TCATGA 1 cut(s) 1387
PceI AGGCCT 1 cut(s) 1810
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PctI GAATGC 1 cut(s) 349
PdiI GCCGGC 1 cut(s) 195
PfeI GAWTC 4 cut(s) 179, 580, 766, 1277
PfoI TCCNGGA 2 cut(s) 526, 703
PinAI ACCGGT 1 cut(s) 1642
PleI GAGTC 6 cut(s) 31, 142, 1018, 1398, 1679, 1829
PluTI GGCGCC 1 cut(s) 285
PpsI GAGTC 6 cut(s) 31, 142, 1018, 1398, 1679, 1829
PpuMI RGGWCCY 2 cut(s) 1153, 1916
PshBI ATTAAT 1 cut(s) 1017
PsiI TTATAA 1 cut(s) 1110
Psp1406I AACGTT 1 cut(s) 1611
Psp5II RGGWCCY 2 cut(s) 1153, 1916
Psp6I CCWGG 5 cut(s) 526, 590, 703, 978, 1810
PspEI GGTNACC 1 cut(s) 1335
PspGI CCWGG 5 cut(s) 526, 590, 703, 978, 1810
PspN4I GGNNCC 6 cut(s) 283, 361, 558, 838, 1890, 1918
PspPI GGNCC 3 cut(s) 1153, 1795, 1916
PspPPI RGGWCCY 2 cut(s) 1153, 1916
PsuI RGATCY 3 cut(s) 27, 903, 1935
PvuII CAGCTG 1 cut(s) 1233
RruI TCGCGA 1 cut(s) 1029
RsaI GTAC 3 cut(s) 630, 1469, 1848
RsaNI GTAC 3 cut(s) 629, 1468, 1847
SaqAI TTAA 7 cut(s) 402, 477, 1017, 1223, 1520, 1805, 1940
Sau3AI GATC 8 cut(s) 27, 427, 748, 903, 993, 1288, 1390, 1935
Sau96I GGNCC 3 cut(s) 1153, 1795, 1916
ScaI AGTACT 1 cut(s) 1469
SchI GAGTC 6 cut(s) 31, 142, 1018, 1398, 1680, 1829
ScrFI CCNGG 5 cut(s) 528, 592, 705, 980, 1812
SfaNI GCATC 4 cut(s) 837, 1240, 1335, 1414
SfoI GGCGCC 1 cut(s) 283
Sfr274I CTCGAG 1 cut(s) 1049
SfuI TTCGAA 2 cut(s) 513, 583
SinI GGWCC 3 cut(s) 1153, 1795, 1916
SlaI CTCGAG 1 cut(s) 1049
SmlI CTYRAG 2 cut(s) 917, 1049
SmoI CTYRAG 2 cut(s) 917, 1049
SpeI ACTAGT 1 cut(s) 643
SphI GCATGC 1 cut(s) 1326
SseBI AGGCCT 1 cut(s) 1810
SspDI GGCGCC 1 cut(s) 281
SspMI CTAG 3 cut(s) 644, 1437, 1592
StuI AGGCCT 1 cut(s) 1810
StyD4I CCNGG 5 cut(s) 526, 590, 703, 978, 1810
TaaI ACNGT 5 cut(s) 304, 453, 720, 1001, 1658
TaiI ACGT 1 cut(s) 1614
TaqI TCGA 4 cut(s) 315, 513, 583, 1050
TaqII GACCGA 1 cut(s) 1812
TatI WGTACW 2 cut(s) 1467, 1846
TauI GCSGC 7 cut(s) 14, 23, 143, 241, 244, 381, 789
TfiI GAWTC 4 cut(s) 179, 580, 766, 1277
Tru1I TTAA 7 cut(s) 402, 477, 1017, 1223, 1520, 1805, 1940
Tru9I TTAA 7 cut(s) 402, 477, 1017, 1223, 1520, 1805, 1940
TscAI CASTG 2 cut(s) 499, 1004
TseFI GTSAC 2 cut(s) 859, 1946
TseI GCWGC 8 cut(s) 161, 1094, 1099, 1233, 1261, 1568, 1571, 1733
Tsp45I GTSAC 2 cut(s) 859, 1946
TspDTI ATGAA 8 cut(s) 17, 37, 79, 712, 716, 1131, 1376, 1622
TspGWI ACGGA 2 cut(s) 191, 868
TspRI CASTG 2 cut(s) 499, 1004
VpaK11BI GGWCC 3 cut(s) 1153, 1795, 1916
VspI ATTAAT 1 cut(s) 1017
XagI CCTNNNNNAGG 1 cut(s) 531
XapI RAATTY 3 cut(s) 296, 585, 1038
XbaI TCTAGA 1 cut(s) 1436
XceI RCATGY 1 cut(s) 1326
XcmI CCANNNNNNNNNTGG 1 cut(s) 1299
XhoI CTCGAG 1 cut(s) 1049
XspI CTAG 3 cut(s) 644, 1437, 1592
ZrmI AGTACT 1 cut(s) 1469
Zsp2I ATGCAT 1 cut(s) 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.