Rw6G033040

methyltransferase PMT10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011820
Physical Location & Seq
Forward (+)
57253646 .. 57257189
3544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G033040.1

Sequence Viewer

Length: 1854 bp
ATGAAGGCCCTCGCGGCCACCGCGGCGGAGATCGTGAAGAACCCGACGTTCATCAAAATTACAGCTATTGCCATCGCCTCCTTCACCCTCTTCGTCCTCGCCAACCACTACTCCGCTTCCTACTCTCCCTTCTCCTCCTCCACCGCCACAACCTCCGCCACTCACTCTCTCGCATCTCCGGCGCCGGAATCCTCTCCACCGACACCTCCCGCGTCTCCTCCACCGCCGCCGCCTTCACCGCCTCCGCCTCCACCCCCGGAGGTGGAGAGGATGGGGATAGTGGACGAGAACGGCGCGATGTCGGAGGAGTTCAAGGTCGGCGAGTTCGACGCGACCCTGGCCGAGGAGCTGAAGAATGCGAGCGGTGGAGTGAAGGATGGGAGTGGCGGTGACGGCAACGGCGGTGGTGTTAGGGTTAAGATTGACAAGTTTGGGGTGTGTGATCAGAAATTCACTGATTATATACCGTGTTTGGACAATGTGGATGAGATTAAGAAGCTGAACTCGACTGAGAGAGGTGAAAAGTTTGAAAGGCATTGTCCTGGACAAGGCAAGAGGCTGAATTGCTTGGTGCCGAGGCCGAAAGGGTATCAGATTCGAATTCCTTGGCCTCAGAGCAGAGATGAGCGATTAAGAAAAGATAAATTTGTGTTTCCTGGAGGTGGAACACAGTTTATTCATGGGGCAAATGAGTACTTGGATCAGATTTCTCAGATGATTCCTGAAATTGCATTTGGCCACAAGACCCGAGTAGCGTTAGATGTTGGTTGTGGAGTAGCGAGTTTTGGTGCCTTTTTGATGCAACGGAATGTGACCACTCTGTCAGTAGCGCCAAAAGATGTCCATGAGAACCAGATCCAGTTTGCACTAGAGCGTGGTGTGCCTGCTATGGTGGCAGTGTTTGCAACTCACCGCTTGTTGTATCCAAGCCAGGCTTTTGACTTGATTCACTGTTCAAGATGTAGAATTAATTGGACTCGTGATGATGGAATTTGGCTTCTCGAGGTTGACAGGTTGCTGAGAGCAGGAGGATATTTTGTTTGGGCAGCGCAGCCTGTTTATAAACATGAAGAAGCCCTACAAGTACAATGGAAAGAAATGGAGGACCTGACTACACGCATTTGCTGGGAACTTATAAAGAAGGAAGGGATGATTGCCATATGGAGGAAACCTTTGAACAACAGCTGCTATCTTAGTCGTGATGTCGAAGTGCAGCCTTCCATTATGTATGTTGGTTTGAAGGCATGCATCACTCAGTTACCAGAAAATGGTTATGGAGCAAATGTTTCTTTGTGGCCTGCAAGGCTTCAGGATCCACCAGACAGACTCCAGAGCATACGATTAGATGCCTATATATCTAGAAAAGAAATCTTCACGGCTGAGGCAAAGTACTGGAATGAAATATTAACAGGTTATGTTGGTGCTTACCATTGGAAGAGTTTTGACCTTGGCAGGTTTGCAGCAGCATTGCATGACCACGGACTAGATTGTTGGGTAATGAATGTTGTTCCCGTTTCTGGGTTCAACACCTTACCAGTTATTTATGACCGTGGACTTATCGGAGTAATGCATGACTGGTGTGAGCCATTTGACACTTATCCAAGAACATATGACTTATTGCATGCAGCGGGTCTCTTCTCTATCGAGCAAAAGAGGTGTAATATCTCAACCATCATTCTTGAAATGGATCGAATGTTAAGGCCTGGGGGACGCGTTTATATACGTGACTCTGTGTCTGTAATCGGTGAGCTTCACGAACTTGCAAATGCGGTTGGATGGGTGCCTGCACTACACGATACCGGAGAAGGACCCCATGCAAGCTGGAAGATCTTAATAGGTGACAAGCGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

617

Amino Acids

68.63

Weight (kDa)

6.3

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 153 - 209 9.3e-18 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_29 PF03141 212 - 615 6.3e-163 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 253 - 347 4.2e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77260
fragaria_vesca FvH4_2g28020
malus_domestica MD15G1132900.v1.1
prunus_persica Prupe.1G488500_v2.0.a1 Prupe.1G488500_v2.0.a1
pyrus_communis pycom15g11980
rosa_chinensis RchiOBHm_Chr6g0296881
rosa_laevigata RLG00000011649
rosa_multiflora Rmu_co8446841.1_g000001 Rmu_sc0033097.1_g000001 Rmu_sc0038066.1_g000001
rosa_roxburghii Rroxscaffold_7G00171060
rosa_rugosa Rorug06G0267200 Rorug06G0267300
rosa_samantha Rh6AG378000 Rh6BG386000 Rh6CG391400 Rh6DG378700
rosa_wichuraiana Rw6G033040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1062, 1136
AasI GACNNNNNNGTC 1 cut(s) 820
Acc36I ACCTGC 1 cut(s) 1443
AccB1I GGYRCC 4 cut(s) 181, 571, 788, 1780
AccB7I CCANNNNNTGG 1 cut(s) 1268
AccBSI CCGCTC 1 cut(s) 363
AccII CGCG 6 cut(s) 14, 23, 212, 296, 332, 1713
AclWI GGATC 5 cut(s) 708, 850, 1307, 1320, 1695
AcoI YGGCCR 3 cut(s) 15, 339, 736
AcsI RAATTY 4 cut(s) 449, 600, 644, 990
AcuI CTGAAG 2 cut(s) 371, 1292
AcyI GRCGYC 1 cut(s) 182
AfaI GTAC 3 cut(s) 695, 1086, 1391
AfiI CCNNNNNNNGG 8 cut(s) 262, 343, 548, 662, 1164, 1268, 1517, 1518
AflIII ACRYGT 1 cut(s) 1711
AgsI TTSAA 7 cut(s) 313, 530, 957, 1177, 1240, 1525, 1682
AhdI GACNNNNNGTC 1 cut(s) 1732
AjnI CCWGG 5 cut(s) 336, 541, 655, 930, 1702
AjuI GAANNNNNNNTTGG 2 cut(s) 717, 749
AluBI AGCT 6 cut(s) 65, 349, 499, 1185, 1750, 1821
AluI AGCT 6 cut(s) 65, 349, 499, 1185, 1750, 1821
Alw26I GTCTC 2 cut(s) 219, 1637
AlwI GGATC 5 cut(s) 708, 850, 1307, 1320, 1695
Ama87I CYCGRG 2 cut(s) 747, 1001
AoxI GGCC 8 cut(s) 6, 15, 339, 578, 608, 736, 1295, 1700
ApeKI GCWGC 7 cut(s) 1046, 1051, 1185, 1213, 1460, 1463, 1625
ApoI RAATTY 4 cut(s) 449, 600, 644, 990
AseI ATTAAT 1 cut(s) 969
AspLEI GCGC 4 cut(s) 184, 296, 832, 1051
AspS9I GGNCC 3 cut(s) 7, 1105, 1808
AsuC2I CCSGG 1 cut(s) 257
AsuHPI GGTGA 7 cut(s) 76, 228, 401, 530, 902, 1757, 1850
AsuII TTCGAA 1 cut(s) 598
AvaI CYCGRG 2 cut(s) 747, 1001
AvaII GGWCC 2 cut(s) 1105, 1808
BalI TGGCCA 1 cut(s) 738
BamHI GGATCC 1 cut(s) 1312
BanI GGYRCC 4 cut(s) 181, 571, 788, 1780
BarI GAAGNNNNNNTAC 2 cut(s) 1062, 1094
BauI CACGAG 1 cut(s) 978
BbvCI CCTCAGC 1 cut(s) 1380
BbvI GCAGC 7 cut(s) 1058, 1063, 1172, 1225, 1472, 1475, 1637
BccI CCATC 6 cut(s) 80, 265, 371, 980, 1679, 1770
BceAI ACGGC 4 cut(s) 307, 409, 415, 1392
BciT130I CCWGG 5 cut(s) 338, 543, 657, 932, 1704
BciVI GTATCC 1 cut(s) 933
BclI TGATCA 1 cut(s) 442
BcnI CCSGG 1 cut(s) 257
BcoDI GTCTC 2 cut(s) 219, 1637
BfaI CTAG 3 cut(s) 869, 1359, 1484
BfoI RGCGCY 2 cut(s) 185, 833
BfuAI ACCTGC 1 cut(s) 1443
BfuI GTATCC 1 cut(s) 933
BglI GCCNNNNNGGC 3 cut(s) 14, 23, 1303
BglII AGATCT 1 cut(s) 1827
BmcAI AGTACT 2 cut(s) 695, 1391
Bme1390I CCNGG 6 cut(s) 257, 338, 543, 657, 932, 1704
Bme18I GGWCC 2 cut(s) 1105, 1808
BmeRI GACNNNNNGTC 1 cut(s) 1732
BmeT110I CYCGRG 2 cut(s) 747, 1001
BmgT120I GGNCC 3 cut(s) 7, 1105, 1808
BmiI GGNNCC 6 cut(s) 183, 573, 790, 1314, 1782, 1810
BmrFI CCNGG 6 cut(s) 257, 338, 543, 657, 932, 1704
BmsI GCATC 4 cut(s) 182, 789, 1257, 1336
BpmI CTGGAG 2 cut(s) 678, 1313
Bpu10I CCTNAGC 1 cut(s) 1380
Bpu14I TTCGAA 1 cut(s) 598
BpuMI CCSGG 1 cut(s) 257
BsaAI YACGTR 1 cut(s) 1724
BsaHI GRCGYC 1 cut(s) 182
BsaI GGTCTC 1 cut(s) 1637
BsaWI WCCGGW 1 cut(s) 1799
BsaXI ACNNNNNCTCC 6 cut(s) 95, 125, 373, 403, 1269, 1299
Bsc4I CCNNNNNNNGG 8 cut(s) 262, 343, 548, 662, 1164, 1268, 1517, 1518
Bse1I ACTGG 4 cut(s) 859, 1397, 1535, 1580
Bse3DI GCAATG 1 cut(s) 1466
BseBI CCWGG 5 cut(s) 338, 543, 657, 932, 1704
BseGI GGATG 5 cut(s) 276, 382, 490, 1155, 1781
BseLI CCNNNNNNNGG 8 cut(s) 262, 343, 548, 662, 1164, 1268, 1517, 1518
BseMI GCAATG 1 cut(s) 1466
BseMII CTCAG 6 cut(s) 501, 626, 725, 1010, 1268, 1371
BseNI ACTGG 4 cut(s) 859, 1397, 1535, 1580
BseRI GAGGAG 5 cut(s) 124, 127, 207, 320, 359
BseXI GCAGC 7 cut(s) 1058, 1063, 1172, 1225, 1472, 1475, 1637
BseYI CCCAGC 1 cut(s) 1125
BsgI GTGCAG 2 cut(s) 1232, 1770
Bsh1236I CGCG 6 cut(s) 14, 23, 212, 296, 332, 1713
BshFI GGCC 8 cut(s) 8, 17, 341, 580, 610, 738, 1297, 1702
BshNI GGYRCC 4 cut(s) 181, 571, 788, 1780
BsiHKCI CYCGRG 2 cut(s) 747, 1001
BsiSI CCGG 4 cut(s) 179, 185, 257, 1800
BslFI GGGAC 1 cut(s) 1722
BslI CCNNNNNNNGG 8 cut(s) 262, 343, 548, 662, 1164, 1268, 1517, 1518
BsmAI GTCTC 2 cut(s) 219, 1637
BsmBI CGTCTC 1 cut(s) 219
BsmFI GGGAC 1 cut(s) 1722
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 8 cut(s) 8, 17, 341, 580, 610, 738, 1297, 1702
Bso31I GGTCTC 1 cut(s) 1637
BsoBI CYCGRG 2 cut(s) 747, 1001
Bsp119I TTCGAA 1 cut(s) 598
Bsp143I GATC 7 cut(s) 30, 442, 700, 855, 1312, 1687, 1827
BspANI GGCC 8 cut(s) 8, 17, 341, 580, 610, 738, 1297, 1702
BspCNI CTCAG 6 cut(s) 502, 625, 724, 1011, 1267, 1372
BspFNI CGCG 6 cut(s) 14, 23, 212, 296, 332, 1713
BspLI GGNNCC 6 cut(s) 183, 573, 790, 1314, 1782, 1810
BspMI ACCTGC 1 cut(s) 1443
BspPI GGATC 5 cut(s) 708, 850, 1307, 1320, 1695
BspT104I TTCGAA 1 cut(s) 598
BspT107I GGYRCC 4 cut(s) 181, 571, 788, 1780
BspTNI GGTCTC 1 cut(s) 1637
BsrBI CCGCTC 1 cut(s) 363
BsrDI GCAATG 1 cut(s) 1466
BsrI ACTGG 4 cut(s) 859, 1397, 1535, 1580
BssMI GATC 7 cut(s) 30, 442, 700, 855, 1312, 1687, 1827
BssNI GRCGYC 1 cut(s) 182
BssSI CACGAG 1 cut(s) 978
BssT1I CCWWGG 2 cut(s) 605, 1447
Bst2BI CACGAG 1 cut(s) 978
Bst2UI CCWGG 5 cut(s) 338, 543, 657, 932, 1704
Bst4CI ACNGT 4 cut(s) 468, 672, 953, 1550
Bst6I CTCTTC 3 cut(s) 95, 1430, 1640
BstACI GRCGYC 1 cut(s) 182
BstAPI GCANNNNNTGC 1 cut(s) 902
BstBAI YACGTR 1 cut(s) 1724
BstBI TTCGAA 1 cut(s) 598
BstC8I GCNNGC 7 cut(s) 361, 885, 1246, 1299, 1623, 1785, 1819
BstDEI CTNAG 7 cut(s) 510, 612, 711, 1019, 1193, 1254, 1380
BstDSI CCRYGG 3 cut(s) 21, 1477, 1549
BstENI CCTNNNNNAGG 2 cut(s) 341, 546
BstF5I GGATG 5 cut(s) 276, 382, 490, 1155, 1781
BstFNI CGCG 6 cut(s) 14, 23, 212, 296, 332, 1713
BstH2I RGCGCY 2 cut(s) 185, 833
BstHHI GCGC 4 cut(s) 184, 296, 832, 1051
BstKTI GATC 7 cut(s) 33, 445, 703, 858, 1315, 1690, 1830
BstMAI GTCTC 2 cut(s) 219, 1637
BstMBI GATC 7 cut(s) 30, 442, 700, 855, 1312, 1687, 1827
BstNI CCWGG 5 cut(s) 338, 543, 657, 932, 1704
BstNSI RCATGY 2 cut(s) 1248, 1625
BstSCI CCNGG 6 cut(s) 255, 336, 541, 655, 930, 1702
BstUI CGCG 6 cut(s) 14, 23, 212, 296, 332, 1713
BstV1I GCAGC 7 cut(s) 1058, 1063, 1172, 1225, 1472, 1475, 1637
BstX2I RGATCY 3 cut(s) 855, 1312, 1827
BstYI RGATCY 3 cut(s) 855, 1312, 1827
BsuI GTATCC 1 cut(s) 933
BsuRI GGCC 8 cut(s) 8, 17, 341, 580, 610, 738, 1297, 1702
BtgI CCRYGG 3 cut(s) 21, 1477, 1549
BtgZI GCGATG 2 cut(s) 58, 311
BtsCI GGATG 5 cut(s) 276, 382, 490, 1155, 1781
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 3 cut(s) 453, 903, 949
BveI ACCTGC 1 cut(s) 1443
Cac8I GCNNGC 7 cut(s) 361, 885, 1246, 1299, 1623, 1785, 1819
CfoI GCGC 4 cut(s) 184, 296, 832, 1051
Cfr13I GGNCC 3 cut(s) 7, 1105, 1808
Cfr42I CCGCGG 1 cut(s) 24
CseI GACGC 3 cut(s) 201, 338, 1719
Csp6I GTAC 3 cut(s) 694, 1085, 1390
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviAII CATG 8 cut(s) 680, 845, 1067, 1245, 1472, 1571, 1622, 1814
CviQI GTAC 3 cut(s) 694, 1085, 1390
DdeI CTNAG 7 cut(s) 510, 612, 711, 1019, 1193, 1254, 1380
DinI GGCGCC 1 cut(s) 183
DpnI GATC 7 cut(s) 32, 444, 702, 857, 1314, 1689, 1829
DpnII GATC 7 cut(s) 30, 442, 700, 855, 1312, 1687, 1827
DrdI GACNNNNNNGTC 1 cut(s) 820
DriI GACNNNNNGTC 1 cut(s) 1732
DseDI GACNNNNNNGTC 1 cut(s) 820
EaeI YGGCCR 3 cut(s) 15, 339, 736
Eam1104I CTCTTC 3 cut(s) 95, 1430, 1640
Eam1105I GACNNNNNGTC 1 cut(s) 1732
EarI CTCTTC 3 cut(s) 95, 1430, 1640
EciI GGCGGA 3 cut(s) 41, 145, 234
Eco130I CCWWGG 2 cut(s) 605, 1447
Eco147I AGGCCT 1 cut(s) 1702
Eco31I GGTCTC 1 cut(s) 1637
Eco47I GGWCC 2 cut(s) 1105, 1808
Eco57I CTGAAG 2 cut(s) 371, 1292
Eco88I CYCGRG 2 cut(s) 747, 1001
EcoNI CCTNNNNNAGG 2 cut(s) 341, 546
EcoO109I RGGNCCY 3 cut(s) 7, 1105, 1808
EcoRI GAATTC 1 cut(s) 600
EcoRII CCWGG 5 cut(s) 336, 541, 655, 930, 1702
EcoT14I CCWWGG 2 cut(s) 605, 1447
EcoT22I ATGCAT 2 cut(s) 1250, 1572
EgeI GGCGCC 1 cut(s) 183
EheI GGCGCC 1 cut(s) 183
ErhI CCWWGG 2 cut(s) 605, 1447
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 8 cut(s) 683, 848, 1070, 1248, 1475, 1574, 1625, 1817
FalI AAGNNNNNCTT 2 cut(s) 919, 951
FaqI GGGAC 1 cut(s) 1722
FatI CATG 8 cut(s) 679, 844, 1066, 1244, 1471, 1570, 1621, 1813
FauI CCCGC 2 cut(s) 217, 1621
FauNDI CATATG 2 cut(s) 1160, 1609
FbaI TGATCA 1 cut(s) 442
FokI GGATG 5 cut(s) 283, 389, 497, 1162, 1788
FspBI CTAG 3 cut(s) 869, 1359, 1484
GlaI GCGC 4 cut(s) 183, 295, 831, 1050
GsaI CCCAGC 1 cut(s) 1129
GsuI CTGGAG 2 cut(s) 678, 1313
HaeII RGCGCY 2 cut(s) 185, 833
HaeIII GGCC 8 cut(s) 8, 17, 341, 580, 610, 738, 1297, 1702
HapII CCGG 4 cut(s) 179, 185, 257, 1800
HgaI GACGC 3 cut(s) 201, 338, 1719
HhaI GCGC 4 cut(s) 184, 296, 832, 1051
Hin1I GRCGYC 1 cut(s) 182
Hin1II CATG 8 cut(s) 683, 848, 1070, 1248, 1475, 1574, 1625, 1817
Hin6I GCGC 4 cut(s) 182, 294, 830, 1049
HinP1I GCGC 4 cut(s) 182, 294, 830, 1049
HincII GTYRAC 1 cut(s) 1009
HindII GTYRAC 1 cut(s) 1009
HinfI GANTC 7 cut(s) 188, 595, 718, 946, 976, 1326, 1727
HpaII CCGG 4 cut(s) 179, 185, 257, 1800
HphI GGTGA 7 cut(s) 76, 228, 401, 530, 902, 1757, 1850
Hpy166II GTNNAC 3 cut(s) 283, 1009, 1553
Hpy188I TCNGA 7 cut(s) 304, 447, 594, 615, 705, 714, 1562
Hpy8I GTNNAC 3 cut(s) 283, 1009, 1553
Hpy99I CGWCG 2 cut(s) 49, 332
HpyAV CCTTC 9 cut(s) 91, 139, 243, 367, 1135, 1139, 1227, 1234, 1799
HpyCH4III ACNGT 4 cut(s) 468, 672, 953, 1550
HpyCH4IV ACGT 2 cut(s) 47, 1723
HpyF3I CTNAG 7 cut(s) 510, 612, 711, 1019, 1193, 1254, 1380
HpySE526I ACGT 2 cut(s) 47, 1723
Hsp92I GRCGYC 1 cut(s) 182
Hsp92II CATG 8 cut(s) 683, 848, 1070, 1248, 1475, 1574, 1625, 1817
HspAI GCGC 4 cut(s) 182, 294, 830, 1049
KasI GGCGCC 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 442
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 7 cut(s) 30, 442, 700, 855, 1312, 1687, 1827
LmnI GCTCC 2 cut(s) 346, 1277
Lsp1109I GCAGC 7 cut(s) 1058, 1063, 1172, 1225, 1472, 1475, 1637
LweI GCATC 4 cut(s) 182, 789, 1257, 1336
MaeI CTAG 3 cut(s) 869, 1359, 1484
MaeII ACGT 2 cut(s) 47, 1723
MaeIII GTNAC 5 cut(s) 389, 811, 1257, 1724, 1838
MalI GATC 7 cut(s) 32, 444, 702, 857, 1314, 1689, 1829
MbiI CCGCTC 1 cut(s) 363
MboI GATC 7 cut(s) 30, 442, 700, 855, 1312, 1687, 1827
MboII GAAGA 8 cut(s) 49, 82, 364, 1082, 1363, 1447, 1627, 1837
MflI RGATCY 3 cut(s) 855, 1312, 1827
MlsI TGGCCA 1 cut(s) 738
MluCI AATT 9 cut(s) 57, 449, 562, 600, 644, 726, 966, 970, 990
MluI ACGCGT 1 cut(s) 1711
MluNI TGGCCA 1 cut(s) 738
Mly113I GGCGCC 1 cut(s) 182
MlyI GAGTC 3 cut(s) 970, 1320, 1721
MmeI TCCRAC 2 cut(s) 282, 1753
Mox20I TGGCCA 1 cut(s) 738
Mph1103I ATGCAT 2 cut(s) 1250, 1572
MscI TGGCCA 1 cut(s) 738
MseI TTAA 7 cut(s) 417, 492, 632, 969, 1406, 1697, 1832
Msp20I TGGCCA 1 cut(s) 738
MspA1I CMGCKG 3 cut(s) 23, 1185, 1628
MspI CCGG 4 cut(s) 179, 185, 257, 1800
MspR9I CCNGG 6 cut(s) 257, 338, 543, 657, 932, 1704
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 5 cut(s) 338, 543, 657, 932, 1704
MvnI CGCG 6 cut(s) 14, 23, 212, 296, 332, 1713
NarI GGCGCC 1 cut(s) 182
NciI CCSGG 1 cut(s) 257
NdeI CATATG 2 cut(s) 1160, 1609
NdeII GATC 7 cut(s) 30, 442, 700, 855, 1312, 1687, 1827
NlaIII CATG 8 cut(s) 683, 848, 1070, 1248, 1475, 1574, 1625, 1817
NlaIV GGNNCC 6 cut(s) 183, 573, 790, 1314, 1782, 1810
NmeAIII GCCGAG 2 cut(s) 367, 600
NmuCI GTSAC 4 cut(s) 389, 811, 1724, 1838
NsiI ATGCAT 2 cut(s) 1250, 1572
NspI RCATGY 2 cut(s) 1248, 1625
NspV TTCGAA 1 cut(s) 598
PaeI GCATGC 2 cut(s) 1248, 1625
PaeR7I CTCGAG 1 cut(s) 1001
PceI AGGCCT 1 cut(s) 1702
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PctI GAATGC 1 cut(s) 361
PfeI GAWTC 4 cut(s) 188, 595, 718, 946
PflMI CCANNNNNTGG 1 cut(s) 1268
PfoI TCCNGGA 2 cut(s) 541, 655
PleI GAGTC 3 cut(s) 970, 1320, 1721
PluTI GGCGCC 1 cut(s) 185
PpsI GAGTC 3 cut(s) 970, 1320, 1721
Ppu21I YACGTR 1 cut(s) 1724
PpuMI RGGWCCY 2 cut(s) 1105, 1808
PshBI ATTAAT 1 cut(s) 969
PsiI TTATAA 2 cut(s) 1062, 1136
Psp5II RGGWCCY 2 cut(s) 1105, 1808
Psp6I CCWGG 5 cut(s) 336, 541, 655, 930, 1702
PspFI CCCAGC 1 cut(s) 1125
PspGI CCWGG 5 cut(s) 336, 541, 655, 930, 1702
PspN4I GGNNCC 6 cut(s) 183, 573, 790, 1314, 1782, 1810
PspPI GGNCC 3 cut(s) 7, 1105, 1808
PspPPI RGGWCCY 2 cut(s) 1105, 1808
PsuI RGATCY 3 cut(s) 855, 1312, 1827
PvuII CAGCTG 1 cut(s) 1185
RsaI GTAC 3 cut(s) 695, 1086, 1391
RsaNI GTAC 3 cut(s) 694, 1085, 1390
SacII CCGCGG 1 cut(s) 24
SaqAI TTAA 7 cut(s) 417, 492, 632, 969, 1406, 1697, 1832
Sau3AI GATC 7 cut(s) 30, 442, 700, 855, 1312, 1687, 1827
Sau96I GGNCC 3 cut(s) 7, 1105, 1808
ScaI AGTACT 2 cut(s) 695, 1391
SchI GAGTC 3 cut(s) 970, 1320, 1721
ScrFI CCNGG 6 cut(s) 257, 338, 543, 657, 932, 1704
SfaNI GCATC 4 cut(s) 182, 789, 1257, 1336
SfiI GGCCNNNNNGGCC 1 cut(s) 14
SfoI GGCGCC 1 cut(s) 183
Sfr274I CTCGAG 1 cut(s) 1001
Sfr303I CCGCGG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 598
SgrBI CCGCGG 1 cut(s) 24
SinI GGWCC 2 cut(s) 1105, 1808
SlaI CTCGAG 1 cut(s) 1001
SmlI CTYRAG 1 cut(s) 1001
SmoI CTYRAG 1 cut(s) 1001
SphI GCATGC 2 cut(s) 1248, 1625
Sse9I AATT 9 cut(s) 57, 449, 562, 600, 644, 726, 966, 970, 990
SseBI AGGCCT 1 cut(s) 1702
SspDI GGCGCC 1 cut(s) 181
SspI AATATT 1 cut(s) 1404
SspMI CTAG 3 cut(s) 869, 1359, 1484
StuI AGGCCT 1 cut(s) 1702
StyD4I CCNGG 6 cut(s) 255, 336, 541, 655, 930, 1702
StyI CCWWGG 2 cut(s) 605, 1447
TaaI ACNGT 4 cut(s) 468, 672, 953, 1550
TaiI ACGT 2 cut(s) 50, 1726
TaqI TCGA 7 cut(s) 327, 506, 598, 1002, 1206, 1644, 1690
TasI AATT 9 cut(s) 57, 449, 562, 600, 644, 726, 966, 970, 990
TatI WGTACW 3 cut(s) 693, 1084, 1389
TauI GCSGC 4 cut(s) 17, 26, 229, 232
TfiI GAWTC 4 cut(s) 188, 595, 718, 946
Tru1I TTAA 7 cut(s) 417, 492, 632, 969, 1406, 1697, 1832
Tru9I TTAA 7 cut(s) 417, 492, 632, 969, 1406, 1697, 1832
TscAI CASTG 3 cut(s) 460, 903, 956
TseFI GTSAC 4 cut(s) 389, 811, 1724, 1838
TseI GCWGC 7 cut(s) 1046, 1051, 1185, 1213, 1460, 1463, 1625
Tsp45I GTSAC 4 cut(s) 389, 811, 1724, 1838
TspDTI ATGAA 6 cut(s) 17, 40, 668, 1083, 1413, 1514
TspGWI ACGGA 2 cut(s) 820, 1494
TspRI CASTG 3 cut(s) 460, 903, 956
Van91I CCANNNNNTGG 1 cut(s) 1268
VpaK11BI GGWCC 2 cut(s) 1105, 1808
VspI ATTAAT 1 cut(s) 969
XagI CCTNNNNNAGG 2 cut(s) 341, 546
XapI RAATTY 4 cut(s) 449, 600, 644, 990
XbaI TCTAGA 1 cut(s) 1358
XceI RCATGY 2 cut(s) 1248, 1625
XhoI CTCGAG 1 cut(s) 1001
XspI CTAG 3 cut(s) 869, 1359, 1484
ZrmI AGTACT 2 cut(s) 695, 1391
Zsp2I ATGCAT 2 cut(s) 1250, 1572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.