RLG00000011649

methyltransferase PMT10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011820
Physical Location & Seq
Reverse (-)
11387968 .. 11391533
3566 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011649

Sequence Viewer

Length: 1965 bp
ATGAAGGCCCTCGCGGCCACCGCGGCGGAGATCTTGAAGACCCCGACGTTCATCAAAATTACAGCTATTGCCATCGCCTCCCTCACCCTCTTCGTCCTCGCCAACCACTACTCCGCTTCCTACTCTCCCTTCTCCTCCACCGCCACCGCCACAACCTCTGCCACTCACTCTCTCGCATCTTCGGCGCCGGAATCCTCTCCACCGACACCTCCCGCGTCTCCTCCACCGCCGCCGCCTCCACCGCCGGAGGTGGAGCGGATGGGGATAGTGGACGAGAACGGCGCGATGTCGGAGGAGTTCAAGGTCGGCGAGTTCGACGCGAGCCTGGCCGAGGAGCTGAAGAATGCGAGCGGTGGAGTGAAGGATGGGAGTGGCGGTGACGGCAATGGCGGTGGTGTTACGGTTAAGATTGACAAGTTTGGGGTGTGTGATCAGAAGTTCACTGATTATATACCGTGTTTGGACAATGTGGATGAGATTAAGAAGCTGAACTCGACTGAGAGAGGTGAAAAGTTTGAAAGGCATTGTCCTGGAGAAGGCAAGAGGCTGAATTGCTTGGTGCCGAGGCCGAAAGGGTATCAGATTCGAATTCCTTGGCCTCAGAGCAGAGATGAGGTGTGGTTCAGTAATGTACCCCATACAAGACTTGTCGAAGATAAAGGTGGTCAAAATTGGATAGCGATTAAGAAAGATAAATTTGTGTTTCCTGGAGGTGGAACACAGTTTATTCATGGGGCAAATGAGTACTTGGATCAGATTTCTCAGATGATTCCTGAAATTGCATTTGGCCGCAAGACCCGAGTAGCGTTAGATGTTGGTTGTGGAGTAGCGAGTTTTGGTGCCTTTTTGATGCAACGGAATGTGACCACTCTGTCAGTGGCGCCCAAAGATGTCCATGAGAACCAGATCCAGTTTGCACTAGAGCGTGGTGTGCCTGCTATGGTGGCAGTATTTGCAACTCACCGCTTGTTGTATCCAAGCCAGGCTTTTGACTTGATCCACTGTTCAAGATGTAGAATTAATTGGACTCGTGATGACGGAATTTGGCTTCTCGAGGTTGACAGGTTGCTGAGAGCGGGAGGATATTTTGTTTGGGCAGCGCAGCCCGTTTATAAACATGAAGAAGCCCTACAAGTACAATGGAAAGAAATGGAGGACCTGACTACACGCATTTGCTGGGAACTTATAAAGAAGGAAGGGATGATTGCCATATGGAGGAAACCTTTGAACAACAGCTGCTATCTTAGTCATGATGCCGAAGTGCAGCCTCCATTATGTGATTCCAATGATGATCCAGACAATGTTTGGTATGTTGGTTTGAAGCCATGTATCACTCGGTTACCTGAAAATGGTTATGGAGCAAATGTTTCTTTGTGGCCTGCAAGGCTTCAGGATCCACCAGACAGACTCCAGAGCATACGATTAGATGCCTATATATCTAGAAAAGAAATCTTCACGGCTGAGGCGAAGTACTGGAATGAAATATTAACAGGTTATGTTGGTGCTTACCATTGGAAAGAGTTAAACTTTAGAAACATAATGGACATGAGGGCTGGATATGGAGGGTTTGCAGCAGCATTGCATGACCACGGGCTAGATTGTTGGGTAATGAATGTTGTTCCCGTTTCTGGGTTCAACACCTTACCAGTTATTTATGACCGTGGACTTATCGGAGTAATGCATGACTGGTGTGAGCCATTTGACACATATCCAAGAACATACGACTTATTGCACGCAGCAGGTCTCTTCTCTATCGAGCAAAAGAGGTGTAATATCTCAACCATCATTCTTGAAATGGACCGAATGTTAAGGCCGGGGGGACGCGTTTATATACGTGACTCTGTGTCTGTAATCGGTGAGCTTCACGAACTTGCAAATGCGGTTGGATGGGTGCCTGCACTACACGATACAGGAGAAGGACCCCATGCAAGCTGGAAGATCTTAATAGGCGACAAGCGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

655

Amino Acids

72.8

Weight (kDa)

5.88

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 149 - 652 2.8e-226 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 270 - 364 4.6e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77260
fragaria_vesca FvH4_2g28020
malus_domestica MD15G1132900.v1.1
prunus_persica Prupe.1G488500_v2.0.a1 Prupe.1G488500_v2.0.a1
pyrus_communis pycom15g11980
rosa_chinensis RchiOBHm_Chr6g0296881
rosa_laevigata RLG00000011649
rosa_multiflora Rmu_co8446841.1_g000001 Rmu_sc0033097.1_g000001 Rmu_sc0038066.1_g000001
rosa_roxburghii Rroxscaffold_7G00171060
rosa_rugosa Rorug06G0267200 Rorug06G0267300
rosa_samantha Rh6AG378000 Rh6BG386000 Rh6CG391400 Rh6DG378700
rosa_wichuraiana Rw6G033040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1113, 1187
AasI GACNNNNNNGTC 1 cut(s) 871
Acc36I ACCTGC 1 cut(s) 1730
AccB1I GGYRCC 5 cut(s) 184, 559, 839, 880, 1891
AccBSI CCGCTC 3 cut(s) 256, 351, 1076
AccII CGCG 6 cut(s) 14, 23, 215, 284, 320, 1824
AclWI GGATC 6 cut(s) 759, 901, 991, 1286, 1388, 1401
AcoI YGGCCR 3 cut(s) 15, 327, 787
AcsI RAATTY 3 cut(s) 588, 695, 1041
AcuI CTGAAG 2 cut(s) 359, 1373
AcyI GRCGYC 2 cut(s) 185, 881
AfaI GTAC 4 cut(s) 633, 746, 1137, 1472
AfiI CCNNNNNNNGG 7 cut(s) 331, 536, 713, 1215, 1349, 1628, 1629
AflIII ACRYGT 1 cut(s) 1822
AgsI TTSAA 8 cut(s) 37, 301, 518, 1008, 1228, 1321, 1636, 1793
AhdI GACNNNNNGTC 1 cut(s) 1843
AjnI CCWGG 4 cut(s) 324, 529, 706, 981
AjuI GAANNNNNNNTTGG 2 cut(s) 768, 800
AluBI AGCT 6 cut(s) 65, 337, 487, 1236, 1861, 1932
AluI AGCT 6 cut(s) 65, 337, 487, 1236, 1861, 1932
Alw26I GTCTC 2 cut(s) 222, 1748
AlwI GGATC 6 cut(s) 759, 901, 991, 1286, 1388, 1401
Ama87I CYCGRG 2 cut(s) 798, 1052
AoxI GGCC 8 cut(s) 6, 15, 327, 566, 596, 787, 1376, 1811
ApeKI GCWGC 7 cut(s) 1097, 1102, 1236, 1264, 1571, 1574, 1736
ApoI RAATTY 3 cut(s) 588, 695, 1041
AseI ATTAAT 1 cut(s) 1020
AspLEI GCGC 4 cut(s) 187, 284, 883, 1102
AspS9I GGNCC 4 cut(s) 7, 1156, 1798, 1919
AsuC2I CCSGG 1 cut(s) 1815
AsuHPI GGTGA 5 cut(s) 76, 389, 518, 953, 1868
AsuII TTCGAA 1 cut(s) 586
AvaI CYCGRG 2 cut(s) 798, 1052
AvaII GGWCC 3 cut(s) 1156, 1798, 1919
BamHI GGATCC 1 cut(s) 1393
BanI GGYRCC 5 cut(s) 184, 559, 839, 880, 1891
BarI GAAGNNNNNNTAC 2 cut(s) 1113, 1145
BauI CACGAG 1 cut(s) 1029
BbsI GAAGAC 1 cut(s) 44
BbvCI CCTCAGC 1 cut(s) 1461
BbvI GCAGC 7 cut(s) 1109, 1114, 1223, 1276, 1583, 1586, 1748
BccI CCATC 5 cut(s) 80, 253, 359, 1790, 1881
BceAI ACGGC 3 cut(s) 295, 397, 1473
BciT130I CCWGG 4 cut(s) 326, 531, 708, 983
BciVI GTATCC 1 cut(s) 984
BclI TGATCA 1 cut(s) 430
BcnI CCSGG 1 cut(s) 1815
BcoDI GTCTC 2 cut(s) 222, 1748
BfaI CTAG 3 cut(s) 920, 1440, 1595
BfoI RGCGCY 2 cut(s) 188, 884
BfuAI ACCTGC 1 cut(s) 1730
BfuI GTATCC 1 cut(s) 984
BglI GCCNNNNNGGC 3 cut(s) 14, 23, 1384
BglII AGATCT 2 cut(s) 30, 1938
BmcAI AGTACT 2 cut(s) 746, 1472
Bme1390I CCNGG 5 cut(s) 326, 531, 708, 983, 1815
Bme18I GGWCC 3 cut(s) 1156, 1798, 1919
BmeRI GACNNNNNGTC 1 cut(s) 1843
BmeT110I CYCGRG 2 cut(s) 798, 1052
BmgT120I GGNCC 4 cut(s) 7, 1156, 1798, 1919
BmiI GGNNCC 7 cut(s) 186, 561, 841, 882, 1395, 1893, 1921
BmrFI CCNGG 5 cut(s) 326, 531, 708, 983, 1815
BmsI GCATC 4 cut(s) 185, 840, 1243, 1417
BpiI GAAGAC 1 cut(s) 44
BpmI CTGGAG 3 cut(s) 552, 729, 1394
Bpu10I CCTNAGC 1 cut(s) 1461
Bpu14I TTCGAA 1 cut(s) 586
BpuMI CCSGG 1 cut(s) 1815
BsaAI YACGTR 1 cut(s) 1835
BsaHI GRCGYC 2 cut(s) 185, 881
BsaI GGTCTC 1 cut(s) 1748
BsaJI CCNNGG 7 cut(s) 21, 330, 563, 593, 1588, 1660, 1814
BsaXI ACNNNNNCTCC 6 cut(s) 95, 125, 361, 391, 1350, 1380
Bsc4I CCNNNNNNNGG 7 cut(s) 331, 536, 713, 1215, 1349, 1628, 1629
Bse1I ACTGG 4 cut(s) 910, 1478, 1646, 1691
Bse3DI GCAATG 2 cut(s) 391, 1577
BseBI CCWGG 4 cut(s) 326, 531, 708, 983
BseDI CCNNGG 7 cut(s) 21, 330, 563, 593, 1588, 1660, 1814
BseGI GGATG 5 cut(s) 264, 370, 478, 1206, 1892
BseLI CCNNNNNNNGG 7 cut(s) 331, 536, 713, 1215, 1349, 1628, 1629
BseMI GCAATG 2 cut(s) 391, 1577
BseMII CTCAG 5 cut(s) 489, 614, 776, 1061, 1452
BseNI ACTGG 4 cut(s) 910, 1478, 1646, 1691
BseRI GAGGAG 4 cut(s) 124, 210, 308, 347
BseXI GCAGC 7 cut(s) 1109, 1114, 1223, 1276, 1583, 1586, 1748
BseYI CCCAGC 1 cut(s) 1176
BsgI GTGCAG 2 cut(s) 1283, 1881
Bsh1236I CGCG 6 cut(s) 14, 23, 215, 284, 320, 1824
BshFI GGCC 8 cut(s) 8, 17, 329, 568, 598, 789, 1378, 1813
BshNI GGYRCC 5 cut(s) 184, 559, 839, 880, 1891
BsiHKCI CYCGRG 2 cut(s) 798, 1052
BsiSI CCGG 3 cut(s) 188, 245, 1814
BslFI GGGAC 1 cut(s) 1833
BslI CCNNNNNNNGG 7 cut(s) 331, 536, 713, 1215, 1349, 1628, 1629
BsmAI GTCTC 2 cut(s) 222, 1748
BsmBI CGTCTC 1 cut(s) 222
BsmFI GGGAC 1 cut(s) 1833
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 8 cut(s) 8, 17, 329, 568, 598, 789, 1378, 1813
Bso31I GGTCTC 1 cut(s) 1748
BsoBI CYCGRG 2 cut(s) 798, 1052
Bsp119I TTCGAA 1 cut(s) 586
Bsp143I GATC 8 cut(s) 30, 430, 751, 906, 996, 1291, 1393, 1938
BspANI GGCC 8 cut(s) 8, 17, 329, 568, 598, 789, 1378, 1813
BspCNI CTCAG 5 cut(s) 490, 613, 775, 1062, 1453
BspFNI CGCG 6 cut(s) 14, 23, 215, 284, 320, 1824
BspHI TCATGA 1 cut(s) 1249
BspLI GGNNCC 7 cut(s) 186, 561, 841, 882, 1395, 1893, 1921
BspMI ACCTGC 1 cut(s) 1730
BspPI GGATC 6 cut(s) 759, 901, 991, 1286, 1388, 1401
BspT104I TTCGAA 1 cut(s) 586
BspT107I GGYRCC 5 cut(s) 184, 559, 839, 880, 1891
BspTNI GGTCTC 1 cut(s) 1748
BsrBI CCGCTC 3 cut(s) 256, 351, 1076
BsrDI GCAATG 2 cut(s) 391, 1577
BsrI ACTGG 4 cut(s) 910, 1478, 1646, 1691
BssECI CCNNGG 7 cut(s) 21, 330, 563, 593, 1588, 1660, 1814
BssMI GATC 8 cut(s) 30, 430, 751, 906, 996, 1291, 1393, 1938
BssNI GRCGYC 2 cut(s) 185, 881
BssSI CACGAG 1 cut(s) 1029
BssT1I CCWWGG 1 cut(s) 593
Bst2BI CACGAG 1 cut(s) 1029
Bst2UI CCWGG 4 cut(s) 326, 531, 708, 983
Bst4CI ACNGT 5 cut(s) 403, 456, 723, 1004, 1661
Bst6I CTCTTC 2 cut(s) 95, 1751
BstACI GRCGYC 2 cut(s) 185, 881
BstAPI GCANNNNNTGC 1 cut(s) 953
BstBAI YACGTR 1 cut(s) 1835
BstBI TTCGAA 1 cut(s) 586
BstC8I GCNNGC 7 cut(s) 322, 349, 936, 1380, 1734, 1896, 1930
BstDEI CTNAG 6 cut(s) 498, 600, 762, 1070, 1244, 1461
BstDSI CCRYGG 3 cut(s) 21, 1588, 1660
BstEII GGTNACC 1 cut(s) 1338
BstENI CCTNNNNNAGG 2 cut(s) 329, 534
BstF5I GGATG 5 cut(s) 264, 370, 478, 1206, 1892
BstFNI CGCG 6 cut(s) 14, 23, 215, 284, 320, 1824
BstH2I RGCGCY 2 cut(s) 188, 884
BstHHI GCGC 4 cut(s) 187, 284, 883, 1102
BstKTI GATC 8 cut(s) 33, 433, 754, 909, 999, 1294, 1396, 1941
BstMAI GTCTC 2 cut(s) 222, 1748
BstMBI GATC 8 cut(s) 30, 430, 751, 906, 996, 1291, 1393, 1938
BstNI CCWGG 4 cut(s) 326, 531, 708, 983
BstPI GGTNACC 1 cut(s) 1338
BstSCI CCNGG 5 cut(s) 324, 529, 706, 981, 1813
BstUI CGCG 6 cut(s) 14, 23, 215, 284, 320, 1824
BstV1I GCAGC 7 cut(s) 1109, 1114, 1223, 1276, 1583, 1586, 1748
BstV2I GAAGAC 1 cut(s) 44
BstX2I RGATCY 4 cut(s) 30, 906, 1393, 1938
BstYI RGATCY 4 cut(s) 30, 906, 1393, 1938
BsuI GTATCC 1 cut(s) 984
BsuRI GGCC 8 cut(s) 8, 17, 329, 568, 598, 789, 1378, 1813
BtgI CCRYGG 3 cut(s) 21, 1588, 1660
BtgZI GCGATG 2 cut(s) 58, 299
BtsCI GGATG 5 cut(s) 264, 370, 478, 1206, 1892
BtsIMutI CAGTG 3 cut(s) 441, 882, 1000
BveI ACCTGC 1 cut(s) 1730
Cac8I GCNNGC 7 cut(s) 322, 349, 936, 1380, 1734, 1896, 1930
CciI TCATGA 1 cut(s) 1249
CfoI GCGC 4 cut(s) 187, 284, 883, 1102
Cfr13I GGNCC 4 cut(s) 7, 1156, 1798, 1919
Cfr42I CCGCGG 1 cut(s) 24
CseI GACGC 3 cut(s) 204, 326, 1830
Csp6I GTAC 4 cut(s) 632, 745, 1136, 1471
CspCI CAANNNNNGTGG 2 cut(s) 373, 408
CviAII CATG 9 cut(s) 731, 896, 1118, 1250, 1326, 1546, 1583, 1682, 1925
CviQI GTAC 4 cut(s) 632, 745, 1136, 1471
DdeI CTNAG 6 cut(s) 498, 600, 762, 1070, 1244, 1461
DinI GGCGCC 2 cut(s) 186, 882
DpnI GATC 8 cut(s) 32, 432, 753, 908, 998, 1293, 1395, 1940
DpnII GATC 8 cut(s) 30, 430, 751, 906, 996, 1291, 1393, 1938
DrdI GACNNNNNNGTC 1 cut(s) 871
DriI GACNNNNNGTC 1 cut(s) 1843
DseDI GACNNNNNNGTC 1 cut(s) 871
EaeI YGGCCR 3 cut(s) 15, 327, 787
Eam1104I CTCTTC 2 cut(s) 95, 1751
Eam1105I GACNNNNNGTC 1 cut(s) 1843
EarI CTCTTC 2 cut(s) 95, 1751
EciI GGCGGA 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 593
Eco31I GGTCTC 1 cut(s) 1748
Eco47I GGWCC 3 cut(s) 1156, 1798, 1919
Eco57I CTGAAG 2 cut(s) 359, 1373
Eco88I CYCGRG 2 cut(s) 798, 1052
Eco91I GGTNACC 1 cut(s) 1338
EcoNI CCTNNNNNAGG 2 cut(s) 329, 534
EcoO109I RGGNCCY 3 cut(s) 7, 1156, 1919
EcoO65I GGTNACC 1 cut(s) 1338
EcoRI GAATTC 1 cut(s) 588
EcoRII CCWGG 4 cut(s) 324, 529, 706, 981
EcoT14I CCWWGG 1 cut(s) 593
EcoT22I ATGCAT 1 cut(s) 1683
EgeI GGCGCC 2 cut(s) 186, 882
EheI GGCGCC 2 cut(s) 186, 882
ErhI CCWWGG 1 cut(s) 593
Esp3I CGTCTC 1 cut(s) 222
FaeI CATG 9 cut(s) 734, 899, 1121, 1253, 1329, 1549, 1586, 1685, 1928
FalI AAGNNNNNCTT 2 cut(s) 970, 1002
FaqI GGGAC 1 cut(s) 1833
FatI CATG 9 cut(s) 730, 895, 1117, 1249, 1325, 1545, 1582, 1681, 1924
FauI CCCGC 2 cut(s) 220, 1069
FauNDI CATATG 1 cut(s) 1211
FbaI TGATCA 1 cut(s) 430
FokI GGATG 5 cut(s) 271, 377, 485, 1213, 1899
FspBI CTAG 3 cut(s) 920, 1440, 1595
GlaI GCGC 4 cut(s) 186, 283, 882, 1101
GsaI CCCAGC 1 cut(s) 1180
GsuI CTGGAG 3 cut(s) 552, 729, 1394
HaeII RGCGCY 2 cut(s) 188, 884
HaeIII GGCC 8 cut(s) 8, 17, 329, 568, 598, 789, 1378, 1813
HapII CCGG 3 cut(s) 188, 245, 1814
HgaI GACGC 3 cut(s) 204, 326, 1830
HhaI GCGC 4 cut(s) 187, 284, 883, 1102
Hin1I GRCGYC 2 cut(s) 185, 881
Hin1II CATG 9 cut(s) 734, 899, 1121, 1253, 1329, 1549, 1586, 1685, 1928
Hin6I GCGC 4 cut(s) 185, 282, 881, 1100
HinP1I GCGC 4 cut(s) 185, 282, 881, 1100
HincII GTYRAC 1 cut(s) 1060
HindII GTYRAC 1 cut(s) 1060
HinfI GANTC 7 cut(s) 191, 583, 769, 1027, 1280, 1407, 1838
HpaII CCGG 3 cut(s) 188, 245, 1814
HphI GGTGA 5 cut(s) 76, 389, 518, 953, 1868
Hpy166II GTNNAC 4 cut(s) 271, 441, 1060, 1664
Hpy188I TCNGA 7 cut(s) 292, 435, 582, 603, 756, 765, 1673
Hpy8I GTNNAC 4 cut(s) 271, 441, 1060, 1664
Hpy99I CGWCG 2 cut(s) 49, 320
HpyAV CCTTC 6 cut(s) 139, 355, 530, 1186, 1190, 1910
HpyCH4III ACNGT 5 cut(s) 403, 456, 723, 1004, 1661
HpyCH4IV ACGT 2 cut(s) 47, 1834
HpyF3I CTNAG 6 cut(s) 498, 600, 762, 1070, 1244, 1461
HpySE526I ACGT 2 cut(s) 47, 1834
Hsp92I GRCGYC 2 cut(s) 185, 881
Hsp92II CATG 9 cut(s) 734, 899, 1121, 1253, 1329, 1549, 1586, 1685, 1928
HspAI GCGC 4 cut(s) 185, 282, 881, 1100
KasI GGCGCC 2 cut(s) 184, 880
Ksp22I TGATCA 1 cut(s) 430
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 8 cut(s) 30, 430, 751, 906, 996, 1291, 1393, 1938
LmnI GCTCC 3 cut(s) 253, 334, 1358
Lsp1109I GCAGC 7 cut(s) 1109, 1114, 1223, 1276, 1583, 1586, 1748
LweI GCATC 4 cut(s) 185, 840, 1243, 1417
MaeI CTAG 3 cut(s) 920, 1440, 1595
MaeII ACGT 2 cut(s) 47, 1834
MaeIII GTNAC 5 cut(s) 377, 397, 862, 1338, 1835
MalI GATC 8 cut(s) 32, 432, 753, 908, 998, 1293, 1395, 1940
MbiI CCGCTC 3 cut(s) 256, 351, 1076
MboI GATC 8 cut(s) 30, 430, 751, 906, 996, 1291, 1393, 1938
MboII GAAGA 9 cut(s) 49, 82, 171, 352, 665, 1133, 1444, 1738, 1948
MflI RGATCY 4 cut(s) 30, 906, 1393, 1938
MluCI AATT 9 cut(s) 57, 550, 588, 670, 695, 777, 1017, 1021, 1041
MluI ACGCGT 1 cut(s) 1822
Mly113I GGCGCC 2 cut(s) 185, 881
MlyI GAGTC 3 cut(s) 1021, 1401, 1832
MmeI TCCRAC 2 cut(s) 270, 1864
Mph1103I ATGCAT 1 cut(s) 1683
MseI TTAA 8 cut(s) 405, 480, 684, 1020, 1487, 1523, 1808, 1943
MspA1I CMGCKG 2 cut(s) 23, 1236
MspI CCGG 3 cut(s) 188, 245, 1814
MspR9I CCNGG 5 cut(s) 326, 531, 708, 983, 1815
Mva1269I GAATGC 1 cut(s) 349
MvaI CCWGG 4 cut(s) 326, 531, 708, 983
MvnI CGCG 6 cut(s) 14, 23, 215, 284, 320, 1824
NarI GGCGCC 2 cut(s) 185, 881
NciI CCSGG 1 cut(s) 1815
NdeI CATATG 1 cut(s) 1211
NdeII GATC 8 cut(s) 30, 430, 751, 906, 996, 1291, 1393, 1938
NlaIII CATG 9 cut(s) 734, 899, 1121, 1253, 1329, 1549, 1586, 1685, 1928
NlaIV GGNNCC 7 cut(s) 186, 561, 841, 882, 1395, 1893, 1921
NmeAIII GCCGAG 2 cut(s) 355, 588
NmuCI GTSAC 3 cut(s) 377, 862, 1835
NsiI ATGCAT 1 cut(s) 1683
NspV TTCGAA 1 cut(s) 586
PaeR7I CTCGAG 1 cut(s) 1052
PagI TCATGA 1 cut(s) 1249
PcsI WCGNNNNNNNCGW 2 cut(s) 312, 1463
PctI GAATGC 1 cut(s) 349
PfeI GAWTC 4 cut(s) 191, 583, 769, 1280
PfoI TCCNGGA 2 cut(s) 529, 706
PleI GAGTC 3 cut(s) 1021, 1401, 1832
PluTI GGCGCC 2 cut(s) 188, 884
PpsI GAGTC 3 cut(s) 1021, 1401, 1832
Ppu21I YACGTR 1 cut(s) 1835
PpuMI RGGWCCY 2 cut(s) 1156, 1919
PshBI ATTAAT 1 cut(s) 1020
PsiI TTATAA 2 cut(s) 1113, 1187
Psp5II RGGWCCY 2 cut(s) 1156, 1919
Psp6I CCWGG 4 cut(s) 324, 529, 706, 981
PspEI GGTNACC 1 cut(s) 1338
PspFI CCCAGC 1 cut(s) 1176
PspGI CCWGG 4 cut(s) 324, 529, 706, 981
PspN4I GGNNCC 7 cut(s) 186, 561, 841, 882, 1395, 1893, 1921
PspPI GGNCC 4 cut(s) 7, 1156, 1798, 1919
PspPPI RGGWCCY 2 cut(s) 1156, 1919
PsuI RGATCY 4 cut(s) 30, 906, 1393, 1938
PvuII CAGCTG 1 cut(s) 1236
RsaI GTAC 4 cut(s) 633, 746, 1137, 1472
RsaNI GTAC 4 cut(s) 632, 745, 1136, 1471
SacII CCGCGG 1 cut(s) 24
SaqAI TTAA 8 cut(s) 405, 480, 684, 1020, 1487, 1523, 1808, 1943
Sau3AI GATC 8 cut(s) 30, 430, 751, 906, 996, 1291, 1393, 1938
Sau96I GGNCC 4 cut(s) 7, 1156, 1798, 1919
ScaI AGTACT 2 cut(s) 746, 1472
SchI GAGTC 3 cut(s) 1021, 1401, 1832
ScrFI CCNGG 5 cut(s) 326, 531, 708, 983, 1815
SfaNI GCATC 4 cut(s) 185, 840, 1243, 1417
SfiI GGCCNNNNNGGCC 1 cut(s) 14
SfoI GGCGCC 2 cut(s) 186, 882
Sfr274I CTCGAG 1 cut(s) 1052
Sfr303I CCGCGG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 586
SgrBI CCGCGG 1 cut(s) 24
SinI GGWCC 3 cut(s) 1156, 1798, 1919
SlaI CTCGAG 1 cut(s) 1052
SmlI CTYRAG 1 cut(s) 1052
SmoI CTYRAG 1 cut(s) 1052
Sse9I AATT 9 cut(s) 57, 550, 588, 670, 695, 777, 1017, 1021, 1041
SspDI GGCGCC 2 cut(s) 184, 880
SspI AATATT 1 cut(s) 1485
SspMI CTAG 3 cut(s) 920, 1440, 1595
StyD4I CCNGG 5 cut(s) 324, 529, 706, 981, 1813
StyI CCWWGG 1 cut(s) 593
TaaI ACNGT 5 cut(s) 403, 456, 723, 1004, 1661
TaiI ACGT 2 cut(s) 50, 1837
TaqI TCGA 6 cut(s) 315, 494, 586, 651, 1053, 1755
TaqII GACCGA 1 cut(s) 1815
TasI AATT 9 cut(s) 57, 550, 588, 670, 695, 777, 1017, 1021, 1041
TatI WGTACW 3 cut(s) 744, 1135, 1470
TauI GCSGC 5 cut(s) 17, 26, 232, 235, 792
TfiI GAWTC 4 cut(s) 191, 583, 769, 1280
Tru1I TTAA 8 cut(s) 405, 480, 684, 1020, 1487, 1523, 1808, 1943
Tru9I TTAA 8 cut(s) 405, 480, 684, 1020, 1487, 1523, 1808, 1943
TscAI CASTG 3 cut(s) 448, 882, 1007
TseFI GTSAC 3 cut(s) 377, 862, 1835
TseI GCWGC 7 cut(s) 1097, 1102, 1236, 1264, 1571, 1574, 1736
Tsp45I GTSAC 3 cut(s) 377, 862, 1835
TspDTI ATGAA 6 cut(s) 17, 40, 719, 1134, 1494, 1625
TspGWI ACGGA 2 cut(s) 871, 1053
TspRI CASTG 3 cut(s) 448, 882, 1007
VpaK11BI GGWCC 3 cut(s) 1156, 1798, 1919
VspI ATTAAT 1 cut(s) 1020
XagI CCTNNNNNAGG 2 cut(s) 329, 534
XapI RAATTY 3 cut(s) 588, 695, 1041
XbaI TCTAGA 1 cut(s) 1439
XcmI CCANNNNNNNNNTGG 2 cut(s) 874, 1302
XhoI CTCGAG 1 cut(s) 1052
XspI CTAG 3 cut(s) 920, 1440, 1595
ZrmI AGTACT 2 cut(s) 746, 1472
Zsp2I ATGCAT 1 cut(s) 1683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.