Rorug06G0267200

methyltransferase PMT10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011820
Physical Location & Seq
Forward (+)
41352959 .. 41358210
5252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0267200.1

Sequence Viewer

Length: 546 bp
ATGAGCAGAAGAACCCGAAGCTCAAGCTCCTTCGACCTGATGAACGGTTCTTGTGGGAGAGAATTGGATTGTATGTTTAAGTGTGCCCAAGAAGATGGGTTTGGCTTCAGTTTGATGCAAGGCCCGGAAATTCTTGATTTGGATTCTATCCCTTTCCCCGAAAGGGCCATGTCATTGGCATTTGATATCCTCAATGACCTTGTGAAGGGATCTGCCAACCAGTACAAAGGAATTGGTGACTGTGTTCAGACTATTGTGAGGGAAGAAGGACCTCCCGCTCTTCTGAAGGTGTCGGATGCAACTGCAGCTGATCCTAAATTGCTGACTGCTGATTTGAGTAAAGAACTGTACCATTACTGGGTAAACATGGTATCGAGAAACAACCGTTTCAGCTTCCTGATTTCATTGCTGCAACTGGTACTAAGAAAATTAGACCTGAGGGGGAATCTGCATTATGGAGACTTGCCTGGCACACATCAAATCATCAATGAGGATCACTATGGGGCACTCATCCACCACCTCTTTTTAGTTCGCAACAAACGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0016740 GO:0016741 GO:0032259 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

181

Amino Acids

20.4

Weight (kDa)

6.29

Isoelectric Point (pI)

52.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77260
fragaria_vesca FvH4_2g28020
malus_domestica MD15G1132900.v1.1
prunus_persica Prupe.1G488500_v2.0.a1 Prupe.1G488500_v2.0.a1
pyrus_communis pycom15g11980
rosa_chinensis RchiOBHm_Chr6g0296881
rosa_laevigata RLG00000011649
rosa_multiflora Rmu_co8446841.1_g000001 Rmu_sc0033097.1_g000001 Rmu_sc0038066.1_g000001
rosa_roxburghii Rroxscaffold_7G00171060
rosa_rugosa Rorug06G0267200 Rorug06G0267300
rosa_samantha Rh6AG378000 Rh6BG386000 Rh6CG391400 Rh6DG378700
rosa_wichuraiana Rw6G033040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 278
AciI CCGC 1 cut(s) 276
AclWI GGATC 3 cut(s) 217, 305, 501
AcsI RAATTY 1 cut(s) 129
AcuI CTGAAG 2 cut(s) 91, 305
AfaI GTAC 3 cut(s) 224, 350, 420
AfiI CCNNNNNNNGG 3 cut(s) 163, 205, 358
AjnI CCWGG 1 cut(s) 466
AjuI GAANNNNNNNTTGG 2 cut(s) 84, 116
AluBI AGCT 4 cut(s) 21, 27, 308, 393
AluI AGCT 4 cut(s) 21, 27, 308, 393
Alw26I GTCTC 1 cut(s) 453
AlwI GGATC 3 cut(s) 217, 305, 501
AoxI GGCC 2 cut(s) 121, 165
ApeKI GCWGC 2 cut(s) 305, 409
ApoI RAATTY 1 cut(s) 129
ArsI GACNNNNNNTTYG 2 cut(s) 316, 348
AspS9I GGNCC 3 cut(s) 122, 165, 269
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 248
AvaII GGWCC 1 cut(s) 269
AxyI CCTNAGG 1 cut(s) 437
BaeGI GKGCMC 2 cut(s) 88, 508
BbvI GCAGC 2 cut(s) 317, 396
BccI CCATC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 468
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 453
BfmI CTRYAG 1 cut(s) 303
BisI GCNGC 2 cut(s) 306, 410
BlsI GCNGC 2 cut(s) 307, 411
Bme1390I CCNGG 2 cut(s) 125, 468
Bme18I GGWCC 1 cut(s) 269
BmgT120I GGNCC 3 cut(s) 122, 165, 269
BmrFI CCNGG 2 cut(s) 125, 468
BmrI ACTGGG 1 cut(s) 367
BmsI GCATC 2 cut(s) 105, 286
BmuI ACTGGG 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 7
BpuMI CCSGG 1 cut(s) 125
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 205, 358
Bse1I ACTGG 3 cut(s) 220, 362, 420
Bse21I CCTNAGG 1 cut(s) 437
Bse3DI GCAATG 1 cut(s) 404
BseBI CCWGG 1 cut(s) 468
BseGI GGATG 2 cut(s) 301, 510
BseLI CCNNNNNNNGG 3 cut(s) 163, 205, 358
BseMI GCAATG 1 cut(s) 404
BseMII CTCAG 1 cut(s) 428
BseNI ACTGG 3 cut(s) 220, 362, 420
BseSI GKGCMC 2 cut(s) 88, 508
BseXI GCAGC 2 cut(s) 317, 396
BshFI GGCC 2 cut(s) 123, 167
BsiSI CCGG 1 cut(s) 125
BslI CCNNNNNNNGG 3 cut(s) 163, 205, 358
BsmAI GTCTC 1 cut(s) 453
BsnI GGCC 2 cut(s) 123, 167
Bsp1286I GDGCHC 2 cut(s) 88, 508
Bsp143I GATC 3 cut(s) 209, 310, 493
BspACI CCGC 1 cut(s) 276
BspANI GGCC 2 cut(s) 123, 167
BspCNI CTCAG 1 cut(s) 429
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 3 cut(s) 217, 305, 501
BspQI GCTCTTC 1 cut(s) 285
BsrBI CCGCTC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 404
BsrI ACTGG 3 cut(s) 220, 362, 420
BssMI GATC 3 cut(s) 209, 310, 493
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 4 cut(s) 47, 242, 348, 386
Bst6I CTCTTC 1 cut(s) 285
BstDEI CTNAG 2 cut(s) 422, 437
BstENI CCTNNNNNAGG 1 cut(s) 203
BstF5I GGATG 2 cut(s) 301, 510
BstKTI GATC 3 cut(s) 212, 313, 496
BstMAI GTCTC 1 cut(s) 453
BstMBI GATC 3 cut(s) 209, 310, 493
BstMWI GCNNNNNNNGC 2 cut(s) 305, 540
BstNI CCWGG 1 cut(s) 468
BstSCI CCNGG 2 cut(s) 123, 466
BstSFI CTRYAG 1 cut(s) 303
BstSLI GKGCMC 2 cut(s) 88, 508
BstV1I GCAGC 2 cut(s) 317, 396
BstX2I RGATCY 1 cut(s) 209
BstXI CCANNNNNNTGG 2 cut(s) 95, 175
BstYI RGATCY 1 cut(s) 209
Bsu36I CCTNAGG 1 cut(s) 437
BsuRI GGCC 2 cut(s) 123, 167
BtsCI GGATG 2 cut(s) 301, 510
Cfr13I GGNCC 3 cut(s) 122, 165, 269
Csp6I GTAC 3 cut(s) 223, 349, 419
CviAII CATG 2 cut(s) 169, 367
CviJI RGCY 7 cut(s) 21, 27, 105, 123, 167, 308, 393
CviKI_1 RGCY 7 cut(s) 21, 27, 105, 123, 167, 308, 393
CviQI GTAC 3 cut(s) 223, 349, 419
DdeI CTNAG 2 cut(s) 422, 437
DpnI GATC 3 cut(s) 211, 312, 495
DpnII GATC 3 cut(s) 209, 310, 493
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 269
Eco57I CTGAAG 2 cut(s) 91, 305
Eco81I CCTNAGG 1 cut(s) 437
EcoNI CCTNNNNNAGG 1 cut(s) 203
EcoO109I RGGNCCY 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 466
EcoRV GATATC 1 cut(s) 187
FaeI CATG 2 cut(s) 172, 370
FaiI YATR 5 cut(s) 74, 170, 368, 456, 501
FatI CATG 2 cut(s) 168, 366
FauI CCCGC 1 cut(s) 283
Fnu4HI GCNGC 2 cut(s) 306, 410
FokI GGATG 2 cut(s) 308, 497
Fsp4HI GCNGC 2 cut(s) 306, 410
GluI GCNGC 2 cut(s) 306, 410
HaeIII GGCC 2 cut(s) 123, 167
HapII CCGG 1 cut(s) 125
Hin1II CATG 2 cut(s) 172, 370
HinfI GANTC 2 cut(s) 143, 445
HpaII CCGG 1 cut(s) 125
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 3 cut(s) 249, 285, 295
Hpy188III TCNNGA 3 cut(s) 134, 375, 397
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 4 cut(s) 40, 199, 260, 280
HpyCH4III ACNGT 4 cut(s) 47, 242, 348, 386
HpyCH4V TGCA 5 cut(s) 118, 299, 305, 412, 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 540
HpyF3I CTNAG 2 cut(s) 422, 437
Hsp92II CATG 2 cut(s) 172, 370
Kzo9I GATC 3 cut(s) 209, 310, 493
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 1 cut(s) 32
LpnPI CCDG 9 cut(s) 50, 138, 233, 343, 401, 410, 449, 453, 480
Lsp1109I GCAGC 2 cut(s) 317, 396
LweI GCATC 2 cut(s) 105, 286
MaeIII GTNAC 1 cut(s) 236
MalI GATC 3 cut(s) 211, 312, 495
MbiI CCGCTC 1 cut(s) 278
MboI GATC 3 cut(s) 209, 310, 493
MboII GAAGA 4 cut(s) 21, 104, 272, 275
MflI RGATCY 1 cut(s) 209
MhlI GDGCHC 2 cut(s) 88, 508
MluCI AATT 5 cut(s) 62, 129, 231, 317, 428
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 6 cut(s) 200, 252, 282, 432, 484, 530
MseI TTAA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 2 cut(s) 125, 468
MvaI CCWGG 1 cut(s) 468
MwoI GCNNNNNNNGC 2 cut(s) 305, 540
NciI CCSGG 1 cut(s) 125
NdeII GATC 3 cut(s) 209, 310, 493
NlaIII CATG 2 cut(s) 172, 370
NmuCI GTSAC 1 cut(s) 236
PciSI GCTCTTC 1 cut(s) 285
PfeI GAWTC 2 cut(s) 143, 445
PkrI GCNGC 2 cut(s) 307, 411
PpuMI RGGWCCY 1 cut(s) 269
Psp5II RGGWCCY 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 466
PspGI CCWGG 1 cut(s) 466
PspPI GGNCC 3 cut(s) 122, 165, 269
PspPPI RGGWCCY 1 cut(s) 269
PstI CTGCAG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 3 cut(s) 224, 350, 420
RsaNI GTAC 3 cut(s) 223, 349, 419
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 2 cut(s) 306, 410
Sau3AI GATC 3 cut(s) 209, 310, 493
Sau96I GGNCC 3 cut(s) 122, 165, 269
ScrFI CCNGG 2 cut(s) 125, 468
SduI GDGCHC 2 cut(s) 88, 508
SfaNI GCATC 2 cut(s) 105, 286
SfcI CTRYAG 1 cut(s) 303
SinI GGWCC 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 22
SmoI CTYRAG 1 cut(s) 22
Sse9I AATT 5 cut(s) 62, 129, 231, 317, 428
SsiI CCGC 1 cut(s) 276
StyD4I CCNGG 2 cut(s) 123, 466
TaaI ACNGT 4 cut(s) 47, 242, 348, 386
TaqI TCGA 2 cut(s) 33, 374
TasI AATT 5 cut(s) 62, 129, 231, 317, 428
TatI WGTACW 1 cut(s) 222
TfiI GAWTC 2 cut(s) 143, 445
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 2 cut(s) 305, 409
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 2 cut(s) 56, 393
VpaK11BI GGWCC 1 cut(s) 269
XagI CCTNNNNNAGG 1 cut(s) 203
XapI RAATTY 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.