FvH4_2g28400

ATP synthase subunit alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009874
Physical Location & Seq
Forward (+)
22324542 .. 22328990
4449 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28400.t1

Sequence Viewer

Length: 2511 bp
ATGGCGACTGCCTCTGCTTCATCTTCCCGCACCAGGACCCGTCCCAGTGGACCTGTCCTTCGCTCTCTCTCACCTTCTGGAAGGTTCTACACCGGCTCCTCCAATGCCTCGATCCAATCCACCGCTTCGGCTTTCGCCTCCTCCACCAGCTCCAGCTTCTCGTCTCCGTCGTCGGCCTTTTTCAACAAACACCAGGACAACCACAGCCACTACAACCACCAGGACTACCACCACCACCGTGCCGCTTCACCGACGCGTGTCAATCTCTACACCTCCAGTGCTCTGTCCCCTGCCGTCCGCTTCTCCATCGAGCACCGCTCCATCTCACCTAACCGTTCCATCACTGCTTCGAAGAAGAACGGCCCCATCGCAGCGTCGAGGAAGACGTGTATGTGCTCCCCGACAACGCATCCGGGGTCGTTCCGCTGCAGTTTGCACAAGAATCAAGGGAGCTCAAATCAGCATCACAACCACACGCCGTATCCCAACAACAGGCTCAATATGCGCAGATCTGCCATGACGAACTCGCTGGTTCGCATCAGCGGCGTCGAGGGCGAGTGGGTGAAGAGAGCCTTGACGGCTCTGATTCGCCCTTCTTCGCATCAGCAGAGGCGTAGAGCTGGGTTCCAGCCACGGCAGAGCCGACTCTCGAGCAGACACAGGTTGGTTATTTTCCGGTCATTTCTCTATTTTTCCGATCTTATATTCCGGCGAGTCATCTCCGAGCAAGCATCGCACCAGATCCGATCAGATCTAAAGGCTTTTGCTCAGTCCAGCTCAACTCGAAGAATTTTGAAGCGGTGGCGCCGAGTCAACCCCGGCGAGTTTTGGGGCTTCTCTACTGAATCGGCAGCTCGAAACACCACTCGTTTGGGGTCCGTTCCGTTCACCACCGTTGTGTTTGCTCGTCCACCGTTCCGTTCAGTTCCGTGGTTTGTTCCGTTCACCGTCCCGTTAAGTTGCGTTGCGTGTGTTACGTTCCGTTTGCTCCGCCGTATTGTTGCGTTGCGTTTATGCCAGAAACTTCTTGCAGCTGGCAGCCATGAGCTAAGACAACGTCGGTTGACGGGGATGAGGCAAATGGGGTCACAATTACAATGTACGGGGTCGAGTAATTTCTTGGTCGTCTGCCGGTACTTGTGGATTCAAGGGTACAAGAAGAGGGACACCATTTGCCGCTCAAACCGCAGCGGGAAATGCTATTCGGACAGTGGTGGATCAAGGTATGCAACGAGCAGAAGTCATGATAAAGGGCCCGGGTCTATTGTGGATGTTCCTGCGGGAAAGGCTCTGCTAGGGCGTGTGGTCGACGGGTTGGGAGTTCCTATTGATGGAAGAGGAGCTCTAAGCGATCACGAGCGAAGACGTGTCGAAGTGAAAGCCCCTGGCATTATTGAACGTAAATCTGTGCACGAGCCTATGCAAACAGGGTTAAAAGCGGTAGATAGCCTGGTTCCTATAGGCCGTGGTCAACGAGAACTTATAATAGGGGACCGACAAACTGGAAAAACGGCTATTGCTATCGATACCATATTAAACCAAAAGCAACTGAACTCAAGGGCCACCTCTGAGAGTGATACATTGTATTGTGTCTATGTAGCAATTGGACAGAAACGCTCAACTGTGGCACAATTAGTTCAAATTCTTTCAGAAGCGAATGCTTTGGAATATTCCATTCTTGTAGCAGCCACCGCTTCGGATCCGGCTCCTCTGCAATTTATGGCCCCATATTCTGGGTGTGCCATGGGGGAATATTTCCGCGATAATGGAATGCACGCATTAATAATCTATGATGATCTTAGTAAACAGGCGGTGGCATATCGACAAATGTCATTATTGTTACGCCGACCACCAGGCCGTGAGGCTTTCCCAGGCGATGTTTTCTATTTACATTCCCGTCTCTTAGAAAGAGCCGCTAAACGATCGGACCAGACAGGTGCAGGTAGCTTGACCGCCTTACCCGTCATTGAAACACAAGCTGGAGACGTATCGGCCTATATTCCAACCAATGTGATCTCCATTACTGATGGACAAATCTGTTCGGAAACAGAGCTCTTTTATCGCGGAATTAGACCTGCTATTAACGTCGGCTTATCTGTCAGTCGCGTCGGGTCTGCCGCTCAGTTGAAAATTATGAAACAAGTCTGCGGGAGTTCAAAACTCGAATTGGCACAATATCGCGAAGTGGCCGCCTTTGCTCAATTTGGGTCAGACCTTGATGCTGCGACTCAGGCATTACTCAATAGAGGTGCAAGGCTTACAGAAGTACCGAAACAACCACAATATGCACCACTTCCAATTGAAAAACAAATTTTAGTCATTTATGCAGCTGTCAATGGATTCTGTGATCGAATGCCACTAGACAGAATCTCTCAATATGAGAGAGCCATTCCAAATAGTATAAAACCAGAATTACTACAATCCCTTTTAGAAAAGGGGGGGTTAACTAACGAAAGAAAGATAGAACCAGATGCATTCTTAAAAGAAAGCGCTTTGCCTTTCCAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko05010 ko05012 ko05016 map00190 map01100 map04714 map05010 map05012 map05016
Pfam Domains
Protein Families
ATP-synt_ab ATP-synt_ab_C ATP-synt_ab_N

Protein Analysis

837

Amino Acids

92.41

Weight (kDa)

10.29

Isoelectric Point (pI)

58.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_ab PF00006 477 - 701 1.3e-77 ATP synthase alpha/beta family, nucleotide-binding domain
ATP-synt_ab_C PF00306 708 - 820 4.2e-37 ATP synthase alpha/beta chain, C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1484
Acc16I TGCGCA 1 cut(s) 506
Acc36I ACCTGC 2 cut(s) 1931, 2083
AccB1I GGYRCC 1 cut(s) 804
AccB7I CCANNNNNTGG 1 cut(s) 1733
AccBSI CCGCTC 3 cut(s) 318, 1179, 2120
AccI GTMKAC 1 cut(s) 1308
AccII CGCG 5 cut(s) 256, 1761, 2064, 2106, 2181
AclWI GGATC 5 cut(s) 106, 736, 1225, 1694, 1707
AcoI YGGCCR 1 cut(s) 2187
AcsI RAATTY 3 cut(s) 789, 1641, 2310
AcyI GRCGYC 2 cut(s) 546, 805
AfaI GTAC 4 cut(s) 1102, 1136, 1154, 2268
AfeI AGCGCT 1 cut(s) 2491
AfiI CCNNNNNNNGG 4 cut(s) 33, 492, 1331, 1733
AflIII ACRYGT 3 cut(s) 254, 386, 1366
AgsI TTSAA 9 cut(s) 184, 796, 1148, 1397, 1640, 1970, 2128, 2157, 2303
AjiI CACGTC 2 cut(s) 387, 1367
AjnI CCWGG 7 cut(s) 32, 192, 219, 1384, 1449, 1852, 1870
AleI CACNNNNGTG 2 cut(s) 237, 896
Alw21I GWGCWC 7 cut(s) 283, 315, 398, 455, 1345, 1413, 2055
Alw26I GTCTC 3 cut(s) 168, 1904, 1977
Alw44I GTGCAC 1 cut(s) 1409
AlwI GGATC 5 cut(s) 106, 736, 1225, 1694, 1707
Ama87I CYCGRG 2 cut(s) 649, 1256
Aor51HI AGCGCT 1 cut(s) 2491
AoxI GGCC 9 cut(s) 174, 361, 1253, 1462, 1560, 1722, 1855, 1992, 2187
ApaI GGGCCC 1 cut(s) 1257
ApaLI GTGCAC 1 cut(s) 1409
ApeKI GCWGC 9 cut(s) 371, 426, 851, 1031, 1038, 1188, 1685, 2222, 2327
ApoI RAATTY 3 cut(s) 789, 1641, 2310
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
AseI ATTAAT 1 cut(s) 1781
AspLEI GCGC 3 cut(s) 507, 807, 2492
AsuC2I CCSGG 4 cut(s) 414, 819, 1257, 1258
AsuHPI GGTGA 6 cut(s) 63, 240, 318, 574, 880, 937
AsuII TTCGAA 1 cut(s) 350
AvaI CYCGRG 2 cut(s) 649, 1256
AvaII GGWCC 5 cut(s) 36, 50, 876, 1492, 1927
BaeGI GKGCMC 2 cut(s) 1257, 1413
BaeI ACNNNNGTAYC 2 cut(s) 2250, 2283
BamHI GGATCC 1 cut(s) 1699
BanI GGYRCC 1 cut(s) 804
BanII GRGCYC 4 cut(s) 455, 1257, 1345, 2055
BauI CACGAG 2 cut(s) 1355, 1412
BbsI GAAGAC 2 cut(s) 389, 1369
Bbv12I GWGCWC 7 cut(s) 283, 315, 398, 455, 1345, 1413, 2055
BbvI GCAGC 9 cut(s) 383, 413, 863, 1043, 1050, 1200, 1697, 2209, 2339
BccI CCATC 6 cut(s) 314, 329, 347, 374, 1325, 2021
BceAI ACGGC 9 cut(s) 278, 376, 463, 594, 650, 978, 1449, 1527, 1842
BciT130I CCWGG 7 cut(s) 34, 194, 221, 1386, 1451, 1854, 1872
BciVI GTATCC 1 cut(s) 492
BcnI CCSGG 4 cut(s) 414, 819, 1257, 1258
BcoDI GTCTC 3 cut(s) 168, 1904, 1977
BfaI CTAG 2 cut(s) 1295, 2360
BfmI CTRYAG 2 cut(s) 427, 1458
BfoI RGCGCY 2 cut(s) 808, 2493
BfuAI ACCTGC 2 cut(s) 1931, 2083
BfuI GTATCC 1 cut(s) 492
BglI GCCNNNNNGGC 1 cut(s) 578
BglII AGATCT 2 cut(s) 509, 751
Bme18I GGWCC 5 cut(s) 36, 50, 876, 1492, 1927
BmeT110I CYCGRG 2 cut(s) 649, 1256
BmgBI CACGTC 2 cut(s) 387, 1367
BmrI ACTGGG 1 cut(s) 39
BmsI GCATC 7 cut(s) 418, 472, 546, 610, 740, 2209, 2461
BmuI ACTGGG 1 cut(s) 39
BoxI GACNNNNGTC 2 cut(s) 257, 1828
BpiI GAAGAC 2 cut(s) 389, 1369
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpmI CTGGAG 3 cut(s) 136, 259, 2001
Bpu14I TTCGAA 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 1540
BpuMI CCSGG 4 cut(s) 414, 819, 1257, 1258
Bsa29I ATCGAT 1 cut(s) 1524
BsaHI GRCGYC 2 cut(s) 546, 805
BsaJI CCNNGG 9 cut(s) 413, 632, 817, 929, 1256, 1384, 1465, 1743, 1870
BsaWI WCCGGW 1 cut(s) 675
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 4 cut(s) 33, 492, 1331, 1733
Bse118I RCCGGY 2 cut(s) 92, 1131
Bse1I ACTGG 3 cut(s) 45, 276, 1507
BseBI CCWGG 7 cut(s) 34, 194, 221, 1386, 1451, 1854, 1872
BseCI ATCGAT 1 cut(s) 1524
BseDI CCNNGG 9 cut(s) 413, 632, 817, 929, 1256, 1384, 1465, 1743, 1870
BseGI GGATG 3 cut(s) 409, 1077, 1276
BseLI CCNNNNNNNGG 4 cut(s) 33, 492, 1331, 1733
BseMII CTCAG 4 cut(s) 782, 1560, 2135, 2243
BseNI ACTGG 3 cut(s) 45, 276, 1507
BseRI GAGGAG 4 cut(s) 88, 130, 1353, 1698
BseSI GKGCMC 2 cut(s) 1257, 1413
BseXI GCAGC 9 cut(s) 383, 413, 863, 1043, 1050, 1200, 1697, 2209, 2339
BseYI CCCAGC 1 cut(s) 620
BsgI GTGCAG 1 cut(s) 1959
Bsh1236I CGCG 5 cut(s) 256, 1761, 2064, 2106, 2181
Bsh1285I CGRYCG 1 cut(s) 1925
BshFI GGCC 9 cut(s) 176, 363, 1255, 1464, 1562, 1724, 1857, 1994, 2189
BshNI GGYRCC 1 cut(s) 804
BshVI ATCGAT 1 cut(s) 1524
BsiEI CGRYCG 1 cut(s) 1925
BsiHKAI GWGCWC 7 cut(s) 283, 315, 398, 455, 1345, 1413, 2055
BsiHKCI CYCGRG 2 cut(s) 649, 1256
BsiSI CCGG 8 cut(s) 93, 413, 676, 709, 819, 1132, 1257, 1703
BslFI GGGAC 5 cut(s) 27, 271, 935, 1178, 1505
BslI CCNNNNNNNGG 4 cut(s) 33, 492, 1331, 1733
BsmAI GTCTC 3 cut(s) 168, 1904, 1977
BsmBI CGTCTC 3 cut(s) 168, 1904, 1977
BsmFI GGGAC 5 cut(s) 27, 271, 935, 1178, 1505
BsmI GAATGC 4 cut(s) 1663, 1776, 2358, 2474
BsnI GGCC 9 cut(s) 176, 363, 1255, 1464, 1562, 1724, 1857, 1994, 2189
BsoBI CYCGRG 2 cut(s) 649, 1256
Bsp119I TTCGAA 1 cut(s) 350
Bsp120I GGGCCC 1 cut(s) 1253
Bsp1286I GDGCHC 8 cut(s) 283, 315, 398, 455, 1257, 1345, 1413, 2055
Bsp19I CCATGG 1 cut(s) 1743
Bsp68I TCGCGA 1 cut(s) 2181
BspANI GGCC 9 cut(s) 176, 363, 1255, 1464, 1562, 1724, 1857, 1994, 2189
BspCNI CTCAG 4 cut(s) 781, 1561, 2134, 2242
BspDI ATCGAT 1 cut(s) 1524
BspFNI CGCG 5 cut(s) 256, 1761, 2064, 2106, 2181
BspHI TCATGA 1 cut(s) 1243
BspMAI CTGCAG 1 cut(s) 431
BspMI ACCTGC 2 cut(s) 1931, 2083
BspPI GGATC 5 cut(s) 106, 736, 1225, 1694, 1707
BspT104I TTCGAA 1 cut(s) 350
BspT107I GGYRCC 1 cut(s) 804
BsrBI CCGCTC 3 cut(s) 318, 1179, 2120
BsrFI RCCGGY 2 cut(s) 92, 1131
BsrI ACTGG 3 cut(s) 45, 276, 1507
BssAI RCCGGY 2 cut(s) 92, 1131
BssECI CCNNGG 9 cut(s) 413, 632, 817, 929, 1256, 1384, 1465, 1743, 1870
BssNI GRCGYC 2 cut(s) 546, 805
BssSI CACGAG 2 cut(s) 1355, 1412
BssT1I CCWWGG 1 cut(s) 1743
Bst2BI CACGAG 2 cut(s) 1355, 1412
Bst2UI CCWGG 7 cut(s) 34, 194, 221, 1386, 1451, 1854, 1872
Bst4CI ACNGT 7 cut(s) 239, 335, 895, 915, 949, 1211, 1624
Bst6I CTCTTC 3 cut(s) 560, 1154, 1330
BstACI GRCGYC 2 cut(s) 546, 805
BstBI TTCGAA 1 cut(s) 350
BstC8I GCNNGC 3 cut(s) 729, 1036, 1776
BstDEI CTNAG 8 cut(s) 768, 1049, 1346, 1569, 1799, 1903, 2121, 2229
BstDSI CCRYGG 4 cut(s) 632, 929, 1465, 1743
BstF5I GGATG 3 cut(s) 409, 1077, 1276
BstFNI CGCG 5 cut(s) 256, 1761, 2064, 2106, 2181
BstH2I RGCGCY 2 cut(s) 808, 2493
BstHHI GCGC 3 cut(s) 507, 807, 2492
BstMAI GTCTC 3 cut(s) 168, 1904, 1977
BstMCI CGRYCG 1 cut(s) 1925
BstNI CCWGG 7 cut(s) 34, 194, 221, 1386, 1451, 1854, 1872
BstPAI GACNNNNGTC 2 cut(s) 257, 1828
BstSFI CTRYAG 2 cut(s) 427, 1458
BstSLI GKGCMC 2 cut(s) 1257, 1413
BstUI CGCG 5 cut(s) 256, 1761, 2064, 2106, 2181
BstV1I GCAGC 9 cut(s) 383, 413, 863, 1043, 1050, 1200, 1697, 2209, 2339
BstV2I GAAGAC 2 cut(s) 389, 1369
BstX2I RGATCY 4 cut(s) 509, 741, 751, 1699
BstXI CCANNNNNNTGG 1 cut(s) 871
BstYI RGATCY 4 cut(s) 509, 741, 751, 1699
Bsu15I ATCGAT 1 cut(s) 1524
BsuI GTATCC 1 cut(s) 492
BsuRI GGCC 9 cut(s) 176, 363, 1255, 1464, 1562, 1724, 1857, 1994, 2189
BsuTUI ATCGAT 1 cut(s) 1524
BtgI CCRYGG 4 cut(s) 632, 929, 1465, 1743
BtgZI GCGATG 3 cut(s) 352, 717, 1890
BtrI CACGTC 2 cut(s) 387, 1367
BtsCI GGATG 3 cut(s) 409, 1077, 1276
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 4 cut(s) 52, 283, 342, 1216
BtuMI TCGCGA 1 cut(s) 2181
BveI ACCTGC 2 cut(s) 1931, 2083
Cac8I GCNNGC 3 cut(s) 729, 1036, 1776
CciI TCATGA 1 cut(s) 1243
CfoI GCGC 3 cut(s) 507, 807, 2492
Cfr10I RCCGGY 2 cut(s) 92, 1131
Cfr9I CCCGGG 1 cut(s) 1256
ClaI ATCGAT 1 cut(s) 1524
CseI GACGC 4 cut(s) 262, 363, 535, 2095
Csp6I GTAC 4 cut(s) 1101, 1135, 1153, 2267
CviAII CATG 4 cut(s) 517, 1043, 1244, 1744
CviQI GTAC 4 cut(s) 1101, 1135, 1153, 2267
DdeI CTNAG 8 cut(s) 768, 1049, 1346, 1569, 1799, 1903, 2121, 2229
DinI GGCGCC 1 cut(s) 806
EaeI YGGCCR 1 cut(s) 2187
Eam1104I CTCTTC 3 cut(s) 560, 1154, 1330
EarI CTCTTC 3 cut(s) 560, 1154, 1330
EciI GGCGGA 1 cut(s) 980
Ecl136II GAGCTC 3 cut(s) 453, 1343, 2053
Eco130I CCWWGG 1 cut(s) 1743
Eco24I GRGCYC 4 cut(s) 455, 1257, 1345, 2055
Eco47I GGWCC 5 cut(s) 36, 50, 876, 1492, 1927
Eco47III AGCGCT 1 cut(s) 2491
Eco53kI GAGCTC 3 cut(s) 453, 1343, 2053
Eco88I CYCGRG 2 cut(s) 649, 1256
EcoICRI GAGCTC 3 cut(s) 453, 1343, 2053
EcoO109I RGGNCCY 2 cut(s) 36, 1253
EcoRII CCWGG 7 cut(s) 32, 192, 219, 1384, 1449, 1852, 1870
EcoT14I CCWWGG 1 cut(s) 1743
EcoT22I ATGCAT 1 cut(s) 2476
EcoT38I GRGCYC 4 cut(s) 455, 1257, 1345, 2055
EgeI GGCGCC 1 cut(s) 806
EheI GGCGCC 1 cut(s) 806
ErhI CCWWGG 1 cut(s) 1743
Esp3I CGTCTC 3 cut(s) 168, 1904, 1977
FaeI CATG 4 cut(s) 520, 1046, 1247, 1747
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FaqI GGGAC 5 cut(s) 27, 271, 935, 1178, 1505
FatI CATG 4 cut(s) 516, 1042, 1243, 1743
FauI CCCGC 4 cut(s) 35, 1184, 1273, 2141
FblI GTMKAC 1 cut(s) 1308
FokI GGATG 3 cut(s) 396, 1084, 1283
FriOI GRGCYC 4 cut(s) 455, 1257, 1345, 2055
FspBI CTAG 2 cut(s) 1295, 2360
FspI TGCGCA 1 cut(s) 506
GlaI GCGC 3 cut(s) 506, 806, 2491
GsaI CCCAGC 1 cut(s) 624
GsuI CTGGAG 3 cut(s) 136, 259, 2001
HaeII RGCGCY 2 cut(s) 808, 2493
HaeIII GGCC 9 cut(s) 176, 363, 1255, 1464, 1562, 1724, 1857, 1994, 2189
HapII CCGG 8 cut(s) 93, 413, 676, 709, 819, 1132, 1257, 1703
HgaI GACGC 4 cut(s) 262, 363, 535, 2095
HhaI GCGC 3 cut(s) 507, 807, 2492
Hin1I GRCGYC 2 cut(s) 546, 805
Hin1II CATG 4 cut(s) 520, 1046, 1247, 1747
Hin6I GCGC 3 cut(s) 505, 805, 2490
HinP1I GCGC 3 cut(s) 505, 805, 2490
HincII GTYRAC 5 cut(s) 814, 1065, 1309, 1472, 2445
HindII GTYRAC 5 cut(s) 814, 1065, 1309, 1472, 2445
HpaI GTTAAC 1 cut(s) 2445
HpaII CCGG 8 cut(s) 93, 413, 676, 709, 819, 1132, 1257, 1703
HphI GGTGA 6 cut(s) 63, 240, 318, 574, 880, 937
Hpy188III TCNNGA 5 cut(s) 78, 649, 1244, 1355, 2180
Hpy99I CGWCG 9 cut(s) 172, 175, 256, 379, 551, 1062, 1313, 2090, 2111
HpyAV CCTTC 4 cut(s) 68, 75, 84, 603
HpyCH4III ACNGT 7 cut(s) 239, 335, 895, 915, 949, 1211, 1624
HpyCH4IV ACGT 7 cut(s) 386, 977, 1057, 1366, 1399, 1986, 2085
HpyF3I CTNAG 8 cut(s) 768, 1049, 1346, 1569, 1799, 1903, 2121, 2229
HpySE526I ACGT 7 cut(s) 386, 977, 1057, 1366, 1399, 1986, 2085
Hsp92I GRCGYC 2 cut(s) 546, 805
Hsp92II CATG 4 cut(s) 520, 1046, 1247, 1747
HspAI GCGC 3 cut(s) 505, 805, 2490
KasI GGCGCC 1 cut(s) 804
KspAI GTTAAC 1 cut(s) 2445
LmnI GCTCC 8 cut(s) 101, 155, 323, 401, 450, 993, 1340, 1711
Lsp1109I GCAGC 9 cut(s) 383, 413, 863, 1043, 1050, 1200, 1697, 2209, 2339
LweI GCATC 7 cut(s) 418, 472, 546, 610, 740, 2209, 2461
MaeI CTAG 2 cut(s) 1295, 2360
MaeII ACGT 7 cut(s) 386, 977, 1057, 1366, 1399, 1986, 2085
MaeIII GTNAC 3 cut(s) 973, 1086, 1839
MbiI CCGCTC 3 cut(s) 318, 1179, 2120
MfeI CAATTG 2 cut(s) 1602, 2298
MflI RGATCY 4 cut(s) 509, 741, 751, 1699
MhlI GDGCHC 8 cut(s) 283, 315, 398, 455, 1257, 1345, 1413, 2055
MluI ACGCGT 1 cut(s) 254
Mly113I GGCGCC 1 cut(s) 805
MlyI GAGTC 4 cut(s) 639, 723, 819, 2221
MmeI TCCRAC 1 cut(s) 2027
Mph1103I ATGCAT 1 cut(s) 2476
MseI TTAA 7 cut(s) 956, 1433, 1535, 1781, 2082, 2444, 2480
MslI CAYNNNNRTG 2 cut(s) 237, 896
MspA1I CMGCKG 5 cut(s) 426, 543, 1034, 1191, 2330
MspI CCGG 8 cut(s) 93, 413, 676, 709, 819, 1132, 1257, 1703
MunI CAATTG 2 cut(s) 1602, 2298
Mva1269I GAATGC 4 cut(s) 1663, 1776, 2358, 2474
MvaI CCWGG 7 cut(s) 34, 194, 221, 1386, 1451, 1854, 1872
MvnI CGCG 5 cut(s) 256, 1761, 2064, 2106, 2181
NarI GGCGCC 1 cut(s) 805
NciI CCSGG 4 cut(s) 414, 819, 1257, 1258
NcoI CCATGG 1 cut(s) 1743
NlaIII CATG 4 cut(s) 520, 1046, 1247, 1747
NmeAIII GCCGAG 1 cut(s) 833
NmuCI GTSAC 1 cut(s) 1086
NruI TCGCGA 1 cut(s) 2181
NsbI TGCGCA 1 cut(s) 506
NsiI ATGCAT 1 cut(s) 2476
NspV TTCGAA 1 cut(s) 350
OliI CACNNNNGTG 2 cut(s) 237, 896
PaeR7I CTCGAG 1 cut(s) 649
PagI TCATGA 1 cut(s) 1243
PcsI WCGNNNNNNNCGW 3 cut(s) 167, 383, 640
PctI GAATGC 4 cut(s) 1663, 1776, 2358, 2474
PfeI GAWTC 6 cut(s) 442, 586, 845, 1144, 2340, 2367
PflFI GACNNNGTC 1 cut(s) 1056
PflMI CCANNNNNTGG 1 cut(s) 1733
Ple19I CGATCG 1 cut(s) 1925
PleI GAGTC 4 cut(s) 639, 722, 818, 2221
PluTI GGCGCC 1 cut(s) 808
PpsI GAGTC 4 cut(s) 639, 722, 818, 2221
PpuMI RGGWCCY 1 cut(s) 36
PshAI GACNNNNGTC 2 cut(s) 257, 1828
PshBI ATTAAT 1 cut(s) 1781
PsiI TTATAA 1 cut(s) 1484
Psp124BI GAGCTC 3 cut(s) 455, 1345, 2055
Psp5II RGGWCCY 1 cut(s) 36
Psp6I CCWGG 7 cut(s) 32, 192, 219, 1384, 1449, 1852, 1870
PspFI CCCAGC 1 cut(s) 620
PspGI CCWGG 7 cut(s) 32, 192, 219, 1384, 1449, 1852, 1870
PspOMI GGGCCC 1 cut(s) 1253
PspPPI RGGWCCY 1 cut(s) 36
PstI CTGCAG 1 cut(s) 431
PsuI RGATCY 4 cut(s) 509, 741, 751, 1699
PsyI GACNNNGTC 1 cut(s) 1056
PvuI CGATCG 1 cut(s) 1925
PvuII CAGCTG 2 cut(s) 1034, 2330
RruI TCGCGA 1 cut(s) 2181
RsaI GTAC 4 cut(s) 1102, 1136, 1154, 2268
RsaNI GTAC 4 cut(s) 1101, 1135, 1153, 2267
RseI CAYNNNNRTG 2 cut(s) 237, 896
SacI GAGCTC 3 cut(s) 455, 1345, 2055
SalI GTCGAC 1 cut(s) 1307
SaqAI TTAA 7 cut(s) 956, 1433, 1535, 1781, 2082, 2444, 2480
SchI GAGTC 4 cut(s) 639, 723, 819, 2221
SduI GDGCHC 8 cut(s) 283, 315, 398, 455, 1257, 1345, 1413, 2055
SfaNI GCATC 7 cut(s) 418, 472, 546, 610, 740, 2209, 2461
SfcI CTRYAG 2 cut(s) 427, 1458
SfoI GGCGCC 1 cut(s) 806
Sfr274I CTCGAG 1 cut(s) 649
SfuI TTCGAA 1 cut(s) 350
SinI GGWCC 5 cut(s) 36, 50, 876, 1492, 1927
SlaI CTCGAG 1 cut(s) 649
SmaI CCCGGG 1 cut(s) 1258
SmiMI CAYNNNNRTG 2 cut(s) 237, 896
SmlI CTYRAG 2 cut(s) 649, 1555
SmoI CTYRAG 2 cut(s) 649, 1555
SspDI GGCGCC 1 cut(s) 804
SspI AATATT 2 cut(s) 1670, 1754
SspMI CTAG 2 cut(s) 1295, 2360
SstI GAGCTC 3 cut(s) 455, 1345, 2055
StyI CCWWGG 1 cut(s) 1743
TaaI ACNGT 7 cut(s) 239, 335, 895, 915, 949, 1211, 1624
TaiI ACGT 7 cut(s) 389, 980, 1060, 1369, 1402, 1989, 2088
TaqII GACCGA 1 cut(s) 1509
TauI GCSGC 6 cut(s) 245, 546, 1179, 1916, 2120, 2192
TfiI GAWTC 6 cut(s) 442, 586, 845, 1144, 2340, 2367
Tru1I TTAA 7 cut(s) 956, 1433, 1535, 1781, 2082, 2444, 2480
Tru9I TTAA 7 cut(s) 956, 1433, 1535, 1781, 2082, 2444, 2480
TscAI CASTG 4 cut(s) 52, 283, 349, 1216
TseFI GTSAC 1 cut(s) 1086
TseI GCWGC 9 cut(s) 371, 426, 851, 1031, 1038, 1188, 1685, 2222, 2327
Tsp45I GTSAC 1 cut(s) 1086
TspDTI ATGAA 2 cut(s) 9, 2150
TspGWI ACGGA 7 cut(s) 156, 868, 873, 908, 918, 930, 971
TspMI CCCGGG 1 cut(s) 1256
TspRI CASTG 4 cut(s) 52, 283, 349, 1216
Tth111I GACNNNGTC 1 cut(s) 1056
Van91I CCANNNNNTGG 1 cut(s) 1733
VneI GTGCAC 1 cut(s) 1409
VpaK11BI GGWCC 5 cut(s) 36, 50, 876, 1492, 1927
VspI ATTAAT 1 cut(s) 1781
XapI RAATTY 3 cut(s) 789, 1641, 2310
XhoI CTCGAG 1 cut(s) 649
XmaI CCCGGG 1 cut(s) 1256
XmiI GTMKAC 1 cut(s) 1308
XspI CTAG 2 cut(s) 1295, 2360
Zsp2I ATGCAT 1 cut(s) 2476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.