Rh5BG208100

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009874
Physical Location & Seq
Forward (+)
24057399 .. 24079547
22149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG208100.1

Sequence Viewer

Length: 3024 bp
ATGAAATTCTCTACAAGAGCTGCGGAACTAACGACTCTATTAGAAAGTAGAATTACCAACTTTTACACGAATTTTCAAGTGGATAAGATCGGTCGAGTGGTCTCAGTTGGAGATGGGATTGCACGTGTTTATGGATTGAACGAGATTCAAGCTGGGGAAATGGTTGAATTTGCCAGCTGTGTGAAAGGAATAGCGTTGAATCTTAAGAATGAGAATATAGGGATTGTTGTCTTTGGTAGTGATTCCGCTATTAAAGAAGGAGATCTTGTCAAGCGCACTGGATATATTGTGAATGTTCCTGCGGGAAAGGCTCTGCTAGGGAGTGTGGTCGACTGGTTGGGAGTTCCTATTGATGGAAGAGGAGCTCTAAGCGATCACGAGCAAAGACCTGTCGAAGTGAAAGCCCCTGGCATTATTAAACCAATTGGACAGAAACGCTCAACTGTGGCACAATTAGTTCAAATTCTTTCAGAAGGGGATGCTATGGAATATTCCATTCTTGTAGTAGCCACCGCTTCGGATCCGGCTCCTCTGCAATTTCTGGCCCCATATTCTAGGTGTGCCATGGGGGAATATTTCTGTGATAATGGAATGCACGCATTAATCATCTATGATGATCTTAGTAAACAGGCGGTGGCATATCGACAAATGTCATTATTGTTACGCGGACCACCAGGCCGTGAAGCTTTCCCAGGCGATGTTTTCTATTTACATTCCCATCTCTTAGAAAGAGCCGCTAAACGATCGGACCAGACAGGTGCAGGTAGCTTGACCGCCTTGCCCGTCATTGAAACACAAGCTGGAGACGTATCGACCTATATTCCAACCAATGTGATCTCCATTACTGATGGACAAATCTGTTCGGAAACAGAACTCTTTTATCGCGAAATTAGACCTACTATTAACGTCGGCTTATCTGTCAGTCGCGTCGGGTCTGCCACTCAGTTGAAAATTATGAAACAAGTCTGCGGTAGTTCAAAACTGGAATTGGCACAATATCGCGAAGTGGCCGCCTTTGCTCAATTTGGGTCAGACCTTGACGCTGCGACTCAGGCATTACTCAATAGAGCGAAAATGGGGTTCGTGATTTCGAACTCTAGGATTGGTTTGGTCTTTGCTTTTGGGTTTCTGGTGCTGATTTGCTTCACTGGGTTTGGGTCTCATGCTCAACTCATTCCTCTACAAGAAGTGAAAACTCTTGAAACGATATCCCAGAAACTCCAGAATTCTTTTTGGAACATTAGCCAAAGTTCTTGCCAAGATGGTGGATCAAGCTTTAATAAGAATATCACGGACAAGATTCTAAGCAATGTCACTTGCGACTGTTCCTTCGCAAACAACTCTTGCCATGTCACAAACATAGTAATGTACGGTCTAAATTTAACTGGTATTCTACCTGAGGAATTTGGAGATCTTACATATCTACAAGAACTTGATCTCACTCTTAACTGCATCAGTGGATCCATTCCTGCAGGCATTTCTCGCGCCCCTCTTAGGAATCTGTCTCTTACAGGAAATCGACTAAGTGGTTCTATTCCAAAGGAAATTGGTGACATTGCTACTCTTACTAAGCTTTTGTTGGAAGATAATCAACTTGAAGGGCCTCTTCCTGAAAACCTTGGGAGTTTGAGTTCCTTGAATAGACTCCTTCTTTCTGCGAATAACTTTACAGGGACAATTCCAGAATCATTTGGAAATCTAAAGAACTTAACCGATTTTAGGATAGATGGGAGCCGAATCTCAGGAAAAATACCTACTTTTATTGGAAACTGGACCAAACTTGACAGACTGGATATGCAAGGCACGTCCATGGAAGGCCCCATTCCTTCCACCATATCTTTGCTGACAAAGTTAACTCAATTGAGGATATCCGATTTGAATGTATCAAGTATGCCTTTTCCTAATTTGGAGGGTTTGAAAATTTTGGAAAGAGTGATTCTGAGAAATTGCTCAATTACCGATTCTATCCCTGGCTACATTGGAGAGCTGACAAAATTAAAGGAATTGGACCTGAGCTTTAACAAGTTGACAGGTGAAGTTCCAACGACCCTTGAGAATCTTGCTGCTCTTGATTACATGTTTTTGACCAACAATTTACTGACTGGAGAAGTACCAAGCTGGATTTTGAGCAACAAAAATAACTTTGATTTGTCTTACAACAATTTTAGCGGATCACCTACAGTTGGTTGTCAACACTTATCAGTGAATTTAATTTCTAGCTTTTCATCTTCACAAAGCCCGTGGTGCTTGCAGAAGGACCTCCCTTGTTCCACAAAAGCCAAATACTATTCTTTGTTTATTAATTGTGGGGGGGGTCGGATGAAATTCAATGGTAATCAGTACGAAGAGGACTTGAGCCCTGAGGGACCGTCAGACTTCATTTCCTCATTTGAGAAGTGGGGTTATAGCAGTACAGGGATTTACATGGGGAAGGAAAAAGCAGATTACACAGCAACAAACACATCACCTCTTAATGGTTCACCAATTTATCAAACTGCCCGCCTTGTCCCGCTCTCACTCAAGTATTATGGCCTTTGCATGCTTAAGGGTAGTTACAAAGTGCAGCTCCACTTCGCTGAAATAAAGTATTCAGATAATGGCACATTCACTGACCTCGGGCAGCGCATATTTGATGTGTCAATTCAAGGAAATTTGGTTTTAAAGGATTTCAATATTATGAAGGAAGCAGGAGGTGCTGGTAAAGACATCGTCAAAGAATTTAATGTCCTTGTTAATGATTTTAATGTTGATATTGGCCTATCTGCTGGAGCTATTGCTGGCATTGTAGTAGCATCATGTGTGTTGATTGTATTGATTTTGGCAGTCCTTCGATATATGGGTTACTTGGGGGGCAAAGACCTTGAAGATAAAGGACTAGGTTTTTCAAGCCCGATAAGATTAGATATTCCTACCGGAGCACCATACATGCAACCTCCTTATCCTGGGATTACCTCTCAAACATATCAGACCGCTGGTGACGGGTTTATACCCAAGCAAGTGGAGCCGGAGAGGTCTTCAAGTTGA

Protein Analysis

1007

Amino Acids

109.35

Weight (kDa)

5.42

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_ab_N PF02874 28 - 94 4e-18 ATP synthase alpha/beta family, beta-barrel domain
ATP-synt_ab PF00006 138 - 308 1.3e-54 ATP synthase alpha/beta family, nucleotide-binding domain
ATP-synt_ab_C PF00306 315 - 358 3.3e-16 ATP synthase alpha/beta chain, C terminal domain
LRR_14 PF23598 582 - 690 1.8e-08 Leucine-rich repeat region
LRR_8 PF13855 642 - 700 2e-07 Leucine rich repeat
Malectin PF11721 764 - 922 2e-28 Malectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 752
AccBSI CCGCTC 1 cut(s) 2512
AccI GTMKAC 1 cut(s) 330
AccII CGCG 5 cut(s) 666, 885, 927, 1002, 1485
AclWI GGATC 6 cut(s) 515, 528, 1276, 1455, 1468, 2179
AcoI YGGCCR 1 cut(s) 1008
AcvI CACGTG 1 cut(s) 125
AfaI GTAC 4 cut(s) 1370, 2112, 2342, 2413
AfiI CCNNNNNNNGG 6 cut(s) 353, 1494, 1719, 2183, 2474, 2942
AflII CTTAAG 2 cut(s) 203, 2543
AflIII ACRYGT 2 cut(s) 124, 2076
AjiI CACGTC 1 cut(s) 1806
AjnI CCWGG 5 cut(s) 406, 673, 691, 1969, 2941
AloI GAACNNNNNNTCC 2 cut(s) 1615, 1647
Alw21I GWGCWC 2 cut(s) 367, 2920
Alw26I GTCTC 4 cut(s) 106, 798, 1164, 1509
AlwI GGATC 6 cut(s) 515, 528, 1276, 1455, 1468, 2179
Ama87I CYCGRG 1 cut(s) 2615
AoxI GGCC 7 cut(s) 543, 676, 1008, 1601, 1816, 2530, 2755
ApeKI GCWGC 5 cut(s) 20, 1043, 2063, 2563, 2620
ArsI GACNNNNNNTTYG 2 cut(s) 1313, 1345
AseI ATTAAT 2 cut(s) 602, 2301
AspLEI GCGC 3 cut(s) 276, 1487, 2625
AspS9I GGNCC 9 cut(s) 544, 668, 748, 1601, 1773, 1817, 2008, 2257, 2366
AsuHPI GGTGA 6 cut(s) 1562, 2045, 2166, 2457, 2472, 2987
AsuII TTCGAA 1 cut(s) 1091
AvaI CYCGRG 1 cut(s) 2615
AvaII GGWCC 6 cut(s) 668, 748, 1773, 2008, 2257, 2366
AxyI CCTNAGG 2 cut(s) 1398, 2361
BamHI GGATCC 2 cut(s) 520, 1460
BanII GRGCYC 2 cut(s) 367, 2360
BauI CACGAG 1 cut(s) 377
BbrPI CACGTG 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 3006
Bbv12I GWGCWC 2 cut(s) 367, 2920
BbvI GCAGC 5 cut(s) 7, 1030, 2050, 2575, 2632
BccI CCATC 6 cut(s) 107, 347, 726, 842, 1256, 1721
BceAI ACGGC 1 cut(s) 663
BciT130I CCWGG 5 cut(s) 408, 675, 693, 1971, 2943
BcoDI GTCTC 4 cut(s) 106, 798, 1164, 1509
BfaI CTAG 5 cut(s) 317, 555, 1098, 2217, 2876
BfmI CTRYAG 2 cut(s) 1470, 2178
BfrI CTTAAG 2 cut(s) 203, 2543
BfuAI ACCTGC 1 cut(s) 752
BglII AGATCT 2 cut(s) 262, 1411
BisI GCNGC 7 cut(s) 21, 735, 1011, 1044, 2064, 2564, 2621
BlsI GCNGC 7 cut(s) 22, 736, 1012, 1045, 2065, 2565, 2622
Bme1390I CCNGG 5 cut(s) 408, 675, 693, 1971, 2943
Bme18I GGWCC 6 cut(s) 668, 748, 1773, 2008, 2257, 2366
BmeT110I CYCGRG 1 cut(s) 2615
BmgBI CACGTC 1 cut(s) 1806
BmgT120I GGNCC 9 cut(s) 544, 668, 748, 1601, 1773, 1817, 2008, 2257, 2366
BmiI GGNNCC 8 cut(s) 522, 528, 546, 1462, 1733, 1819, 2367, 3003
BmrFI CCNGG 5 cut(s) 408, 675, 693, 1971, 2943
BmrI ACTGGG 1 cut(s) 1158
BmsI GCATC 3 cut(s) 469, 1461, 2801
BmuI ACTGGG 1 cut(s) 1158
BoxI GACNNNNGTC 1 cut(s) 649
BpiI GAAGAC 1 cut(s) 3006
BpmI CTGGAG 4 cut(s) 822, 1205, 2124, 2787
Bpu10I CCTNAGC 1 cut(s) 2012
Bpu14I TTCGAA 1 cut(s) 1091
BpuEI CTTGAG 3 cut(s) 2072, 2374, 2504
BsaAI YACGTR 1 cut(s) 125
BsaI GGTCTC 2 cut(s) 106, 1164
BsaJI CCNNGG 9 cut(s) 406, 564, 691, 1618, 1809, 1969, 2240, 2614, 2942
BsaWI WCCGGW 1 cut(s) 2912
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 1615, 1645
Bsc4I CCNNNNNNNGG 6 cut(s) 353, 1494, 1719, 2183, 2474, 2942
Bse1I ACTGG 8 cut(s) 283, 338, 987, 1153, 1390, 1775, 1794, 2107
Bse21I CCTNAGG 2 cut(s) 1398, 2361
Bse3DI GCAATG 2 cut(s) 1315, 1554
BseBI CCWGG 5 cut(s) 408, 675, 693, 1971, 2943
BseDI CCNNGG 9 cut(s) 406, 564, 691, 1618, 1809, 1969, 2240, 2614, 2942
BseGI GGATG 2 cut(s) 484, 2325
BseLI CCNNNNNNNGG 6 cut(s) 353, 1494, 1719, 2183, 2474, 2942
BseMI GCAATG 2 cut(s) 1315, 1554
BseMII CTCAG 8 cut(s) 117, 956, 1064, 1389, 1755, 1931, 2003, 2352
BseNI ACTGG 8 cut(s) 283, 338, 987, 1153, 1390, 1775, 1794, 2107
BseRI GAGGAG 2 cut(s) 375, 519
BseXI GCAGC 5 cut(s) 7, 1030, 2050, 2575, 2632
BseYI CCCAGC 1 cut(s) 152
BsgI GTGCAG 2 cut(s) 780, 2582
Bsh1236I CGCG 5 cut(s) 666, 885, 927, 1002, 1485
Bsh1285I CGRYCG 2 cut(s) 94, 746
BshFI GGCC 7 cut(s) 545, 678, 1010, 1603, 1818, 2532, 2757
BsiEI CGRYCG 2 cut(s) 94, 746
BsiHKAI GWGCWC 2 cut(s) 367, 2920
BsiHKCI CYCGRG 1 cut(s) 2615
BsiSI CCGG 3 cut(s) 524, 2913, 3005
BslFI GGGAC 3 cut(s) 1687, 2379, 2492
BslI CCNNNNNNNGG 6 cut(s) 353, 1494, 1719, 2183, 2474, 2942
BsmAI GTCTC 4 cut(s) 106, 798, 1164, 1509
BsmBI CGTCTC 1 cut(s) 798
BsmFI GGGAC 3 cut(s) 1687, 2379, 2492
BsmI GAATGC 1 cut(s) 597
BsnI GGCC 7 cut(s) 545, 678, 1010, 1603, 1818, 2532, 2757
Bso31I GGTCTC 2 cut(s) 106, 1164
BsoBI CYCGRG 1 cut(s) 2615
Bsp119I TTCGAA 1 cut(s) 1091
Bsp1286I GDGCHC 3 cut(s) 367, 2360, 2920
Bsp19I CCATGG 2 cut(s) 564, 1809
Bsp68I TCGCGA 2 cut(s) 885, 1002
BspANI GGCC 7 cut(s) 545, 678, 1010, 1603, 1818, 2532, 2757
BspCNI CTCAG 8 cut(s) 116, 955, 1063, 1390, 1754, 1932, 2004, 2353
BspFNI CGCG 5 cut(s) 666, 885, 927, 1002, 1485
BspLI GGNNCC 8 cut(s) 522, 528, 546, 1462, 1733, 1819, 2367, 3003
BspMAI CTGCAG 1 cut(s) 1474
BspMI ACCTGC 1 cut(s) 752
BspPI GGATC 6 cut(s) 515, 528, 1276, 1455, 1468, 2179
BspT104I TTCGAA 1 cut(s) 1091
BspTI CTTAAG 2 cut(s) 203, 2543
BspTNI GGTCTC 2 cut(s) 106, 1164
BsrBI CCGCTC 1 cut(s) 2512
BsrDI GCAATG 2 cut(s) 1315, 1554
BsrI ACTGG 8 cut(s) 283, 338, 987, 1153, 1390, 1775, 1794, 2107
BssECI CCNNGG 9 cut(s) 406, 564, 691, 1618, 1809, 1969, 2240, 2614, 2942
BssSI CACGAG 1 cut(s) 377
BssT1I CCWWGG 3 cut(s) 564, 1618, 1809
Bst2BI CACGAG 1 cut(s) 377
Bst2UI CCWGG 5 cut(s) 408, 675, 693, 1971, 2943
Bst4CI ACNGT 5 cut(s) 445, 1325, 1373, 2182, 2370
Bst6I CTCTTC 3 cut(s) 352, 1611, 2340
BstAFI CTTAAG 2 cut(s) 203, 2543
BstAPI GCANNNNNTGC 1 cut(s) 2693
BstBAI YACGTR 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 1091
BstC8I GCNNGC 7 cut(s) 175, 597, 1474, 2249, 2500, 2540, 2779
BstDSI CCRYGG 3 cut(s) 564, 1809, 2240
BstF5I GGATG 2 cut(s) 484, 2325
BstFNI CGCG 5 cut(s) 666, 885, 927, 1002, 1485
BstHHI GCGC 3 cut(s) 276, 1487, 2625
BstMAI GTCTC 4 cut(s) 106, 798, 1164, 1509
BstMCI CGRYCG 2 cut(s) 94, 746
BstMWI GCNNNNNNNGC 7 cut(s) 308, 1016, 1052, 1482, 2244, 2693, 3001
BstNI CCWGG 5 cut(s) 408, 675, 693, 1971, 2943
BstNSI RCATGY 3 cut(s) 2080, 2542, 2929
BstPAI GACNNNNGTC 1 cut(s) 649
BstSCI CCNGG 5 cut(s) 406, 673, 691, 1969, 2941
BstSFI CTRYAG 2 cut(s) 1470, 2178
BstUI CGCG 5 cut(s) 666, 885, 927, 1002, 1485
BstV1I GCAGC 5 cut(s) 7, 1030, 2050, 2575, 2632
BstV2I GAAGAC 1 cut(s) 3006
BstX2I RGATCY 4 cut(s) 262, 520, 1411, 1460
BstXI CCANNNNNNTGG 2 cut(s) 1265, 2998
BstYI RGATCY 4 cut(s) 262, 520, 1411, 1460
Bsu36I CCTNAGG 2 cut(s) 1398, 2361
BsuRI GGCC 7 cut(s) 545, 678, 1010, 1603, 1818, 2532, 2757
BtgI CCRYGG 3 cut(s) 564, 1809, 2240
BtgZI GCGATG 1 cut(s) 711
BtrI CACGTC 1 cut(s) 1806
BtsCI GGATG 2 cut(s) 484, 2325
BtsIMutI CAGTG 5 cut(s) 276, 1146, 1462, 2208, 2607
BtuMI TCGCGA 2 cut(s) 885, 1002
BveI ACCTGC 1 cut(s) 752
Cac8I GCNNGC 7 cut(s) 175, 597, 1474, 2249, 2500, 2540, 2779
CfoI GCGC 3 cut(s) 276, 1487, 2625
Cfr13I GGNCC 9 cut(s) 544, 668, 748, 1601, 1773, 1817, 2008, 2257, 2366
CseI GACGC 2 cut(s) 916, 1049
Csp6I GTAC 4 cut(s) 1369, 2111, 2341, 2412
CspCI CAANNNNNGTGG 2 cut(s) 2222, 2257
CviAII CATG 9 cut(s) 565, 1163, 1349, 1810, 2077, 2425, 2539, 2796, 2926
CviQI GTAC 4 cut(s) 1369, 2111, 2341, 2412
DraI TTTAAA 1 cut(s) 2661
EaeI YGGCCR 1 cut(s) 1008
Eam1104I CTCTTC 3 cut(s) 352, 1611, 2340
EarI CTCTTC 3 cut(s) 352, 1611, 2340
Ecl136II GAGCTC 1 cut(s) 365
Eco130I CCWWGG 3 cut(s) 564, 1618, 1809
Eco24I GRGCYC 2 cut(s) 367, 2360
Eco31I GGTCTC 2 cut(s) 106, 1164
Eco32I GATATC 2 cut(s) 1209, 1869
Eco47I GGWCC 6 cut(s) 668, 748, 1773, 2008, 2257, 2366
Eco53kI GAGCTC 1 cut(s) 365
Eco72I CACGTG 1 cut(s) 125
Eco81I CCTNAGG 2 cut(s) 1398, 2361
Eco88I CYCGRG 1 cut(s) 2615
EcoICRI GAGCTC 1 cut(s) 365
EcoO109I RGGNCCY 3 cut(s) 1601, 1817, 2257
EcoRI GAATTC 1 cut(s) 1225
EcoRII CCWGG 5 cut(s) 406, 673, 691, 1969, 2941
EcoRV GATATC 2 cut(s) 1209, 1869
EcoT14I CCWWGG 3 cut(s) 564, 1618, 1809
EcoT38I GRGCYC 2 cut(s) 367, 2360
ErhI CCWWGG 3 cut(s) 564, 1618, 1809
Esp3I CGTCTC 1 cut(s) 798
FaeI CATG 9 cut(s) 568, 1166, 1352, 1813, 2080, 2428, 2542, 2799, 2929
FalI AAGNNNNNCTT 6 cut(s) 249, 281, 1590, 1622, 1879, 1911
FaqI GGGAC 3 cut(s) 1687, 2379, 2492
FatI CATG 9 cut(s) 564, 1162, 1348, 1809, 2076, 2424, 2538, 2795, 2925
FauI CCCGC 3 cut(s) 295, 2507, 2517
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 7 cut(s) 21, 735, 1011, 1044, 2064, 2564, 2621
FokI GGATG 2 cut(s) 491, 2332
FriOI GRGCYC 2 cut(s) 367, 2360
Fsp4HI GCNGC 7 cut(s) 21, 735, 1011, 1044, 2064, 2564, 2621
FspBI CTAG 5 cut(s) 317, 555, 1098, 2217, 2876
GlaI GCGC 3 cut(s) 275, 1486, 2624
GluI GCNGC 7 cut(s) 21, 735, 1011, 1044, 2064, 2564, 2621
GsaI CCCAGC 1 cut(s) 156
GsuI CTGGAG 4 cut(s) 822, 1205, 2124, 2787
HaeIII GGCC 7 cut(s) 545, 678, 1010, 1603, 1818, 2532, 2757
HapII CCGG 3 cut(s) 524, 2913, 3005
HgaI GACGC 2 cut(s) 916, 1049
HhaI GCGC 3 cut(s) 276, 1487, 2625
Hin1II CATG 9 cut(s) 568, 1166, 1352, 1813, 2080, 2428, 2542, 2799, 2929
Hin6I GCGC 3 cut(s) 274, 1485, 2623
HinP1I GCGC 3 cut(s) 274, 1485, 2623
HincII GTYRAC 4 cut(s) 331, 1854, 2028, 2192
HindII GTYRAC 4 cut(s) 331, 1854, 2028, 2192
HindIII AAGCTT 3 cut(s) 684, 1273, 1571
HpaI GTTAAC 1 cut(s) 1854
HpaII CCGG 3 cut(s) 524, 2913, 3005
HphI GGTGA 6 cut(s) 1562, 2045, 2166, 2457, 2472, 2987
Hpy166II GTNNAC 6 cut(s) 331, 626, 1854, 2028, 2192, 2480
Hpy8I GTNNAC 6 cut(s) 331, 626, 1854, 2028, 2192, 2480
Hpy99I CGWCG 2 cut(s) 911, 932
HpyCH4III ACNGT 5 cut(s) 445, 1325, 1373, 2182, 2370
HpyCH4IV ACGT 4 cut(s) 124, 807, 906, 1805
HpyF10VI GCNNNNNNNGC 7 cut(s) 308, 1016, 1052, 1482, 2244, 2693, 3001
HpySE526I ACGT 4 cut(s) 124, 807, 906, 1805
Hsp92II CATG 9 cut(s) 568, 1166, 1352, 1813, 2080, 2428, 2542, 2799, 2929
HspAI GCGC 3 cut(s) 274, 1485, 2623
KspAI GTTAAC 1 cut(s) 1854
LmnI GCTCC 7 cut(s) 362, 532, 1731, 2571, 2768, 2915, 3001
Lsp1109I GCAGC 5 cut(s) 7, 1030, 2050, 2575, 2632
LweI GCATC 3 cut(s) 469, 1461, 2801
MaeI CTAG 5 cut(s) 317, 555, 1098, 2217, 2876
MaeII ACGT 4 cut(s) 124, 807, 906, 1805
MaeIII GTNAC 7 cut(s) 660, 1312, 1351, 1550, 2552, 2840, 2975
MbiI CCGCTC 1 cut(s) 2512
MboII GAAGA 7 cut(s) 369, 1595, 1598, 2220, 2357, 2876, 3006
MfeI CAATTG 2 cut(s) 423, 1859
MflI RGATCY 4 cut(s) 262, 520, 1411, 1460
MhlI GDGCHC 3 cut(s) 367, 2360, 2920
MlyI GAGTC 3 cut(s) 28, 1042, 1638
MmeI TCCRAC 5 cut(s) 88, 848, 1560, 2066, 2297
MslI CAYNNNNRTG 2 cut(s) 1364, 1808
MspA1I CMGCKG 2 cut(s) 177, 2972
MspCI CTTAAG 2 cut(s) 203, 2543
MspI CCGG 3 cut(s) 524, 2913, 3005
MspR9I CCNGG 5 cut(s) 408, 675, 693, 1971, 2943
MunI CAATTG 2 cut(s) 423, 1859
Mva1269I GAATGC 1 cut(s) 597
MvaI CCWGG 5 cut(s) 408, 675, 693, 1971, 2943
MvnI CGCG 5 cut(s) 666, 885, 927, 1002, 1485
MwoI GCNNNNNNNGC 7 cut(s) 308, 1016, 1052, 1482, 2244, 2693, 3001
NcoI CCATGG 2 cut(s) 564, 1809
NlaIII CATG 9 cut(s) 568, 1166, 1352, 1813, 2080, 2428, 2542, 2799, 2929
NlaIV GGNNCC 8 cut(s) 522, 528, 546, 1462, 1733, 1819, 2367, 3003
NmuCI GTSAC 4 cut(s) 1312, 1351, 1550, 2975
NruI TCGCGA 2 cut(s) 885, 1002
NspI RCATGY 3 cut(s) 2080, 2542, 2929
NspV TTCGAA 1 cut(s) 1091
PaeI GCATGC 1 cut(s) 2542
PciI ACATGT 1 cut(s) 2076
PctI GAATGC 1 cut(s) 597
PflFI GACNNNGTC 1 cut(s) 2708
PkrI GCNGC 7 cut(s) 22, 736, 1012, 1045, 2065, 2565, 2622
Ple19I CGATCG 1 cut(s) 746
PleI GAGTC 3 cut(s) 28, 1042, 1638
PmaCI CACGTG 1 cut(s) 125
PmlI CACGTG 1 cut(s) 125
PpsI GAGTC 3 cut(s) 28, 1042, 1638
Ppu21I YACGTR 1 cut(s) 125
PpuMI RGGWCCY 1 cut(s) 2257
PscI ACATGT 1 cut(s) 2076
PshAI GACNNNNGTC 1 cut(s) 649
PshBI ATTAAT 2 cut(s) 602, 2301
Psp124BI GAGCTC 1 cut(s) 367
Psp5II RGGWCCY 1 cut(s) 2257
Psp6I CCWGG 5 cut(s) 406, 673, 691, 1969, 2941
PspCI CACGTG 1 cut(s) 125
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 5 cut(s) 406, 673, 691, 1969, 2941
PspN4I GGNNCC 8 cut(s) 522, 528, 546, 1462, 1733, 1819, 2367, 3003
PspPI GGNCC 9 cut(s) 544, 668, 748, 1601, 1773, 1817, 2008, 2257, 2366
PspPPI RGGWCCY 1 cut(s) 2257
PstI CTGCAG 1 cut(s) 1474
PsuI RGATCY 4 cut(s) 262, 520, 1411, 1460
PsyI GACNNNGTC 1 cut(s) 2708
PvuI CGATCG 1 cut(s) 746
PvuII CAGCTG 1 cut(s) 177
RruI TCGCGA 2 cut(s) 885, 1002
RsaI GTAC 4 cut(s) 1370, 2112, 2342, 2413
RsaNI GTAC 4 cut(s) 1369, 2111, 2341, 2412
RseI CAYNNNNRTG 2 cut(s) 1364, 1808
SacI GAGCTC 1 cut(s) 367
SalI GTCGAC 1 cut(s) 329
SatI GCNGC 7 cut(s) 21, 735, 1011, 1044, 2064, 2564, 2621
Sau96I GGNCC 9 cut(s) 544, 668, 748, 1601, 1773, 1817, 2008, 2257, 2366
SbfI CCTGCAGG 1 cut(s) 1474
SchI GAGTC 3 cut(s) 28, 1042, 1638
ScrFI CCNGG 5 cut(s) 408, 675, 693, 1971, 2943
SdaI CCTGCAGG 1 cut(s) 1474
SduI GDGCHC 3 cut(s) 367, 2360, 2920
SfaNI GCATC 3 cut(s) 469, 1461, 2801
SfcI CTRYAG 2 cut(s) 1470, 2178
SfuI TTCGAA 1 cut(s) 1091
SinI GGWCC 6 cut(s) 668, 748, 1773, 2008, 2257, 2366
SmiMI CAYNNNNRTG 2 cut(s) 1364, 1808
SmlI CTYRAG 5 cut(s) 203, 2051, 2353, 2519, 2543
SmoI CTYRAG 5 cut(s) 203, 2051, 2353, 2519, 2543
SphI GCATGC 1 cut(s) 2542
Sse8387I CCTGCAGG 1 cut(s) 1474
SspI AATATT 3 cut(s) 491, 575, 2674
SspMI CTAG 5 cut(s) 317, 555, 1098, 2217, 2876
SstI GAGCTC 1 cut(s) 367
StyD4I CCNGG 5 cut(s) 406, 673, 691, 1969, 2941
StyI CCWWGG 3 cut(s) 564, 1618, 1809
TaaI ACNGT 5 cut(s) 445, 1325, 1373, 2182, 2370
TaiI ACGT 4 cut(s) 127, 810, 909, 1808
TaqI TCGA 8 cut(s) 94, 330, 393, 643, 812, 1091, 1519, 2830
TaqII GACCGA 1 cut(s) 80
TatI WGTACW 1 cut(s) 2411
TauI GCSGC 2 cut(s) 737, 1013
TscAI CASTG 5 cut(s) 283, 1153, 1462, 2208, 2614
TseFI GTSAC 4 cut(s) 1312, 1351, 1550, 2975
TseI GCWGC 5 cut(s) 20, 1043, 2063, 2563, 2620
Tsp45I GTSAC 4 cut(s) 1312, 1351, 1550, 2975
TspDTI ATGAA 6 cut(s) 17, 971, 2214, 2336, 2368, 2693
TspGWI ACGGA 1 cut(s) 1307
TspRI CASTG 5 cut(s) 283, 1153, 1462, 2208, 2614
Tth111I GACNNNGTC 1 cut(s) 2708
Vha464I CTTAAG 2 cut(s) 203, 2543
VpaK11BI GGWCC 6 cut(s) 668, 748, 1773, 2008, 2257, 2366
VspI ATTAAT 2 cut(s) 602, 2301
XceI RCATGY 3 cut(s) 2080, 2542, 2929
XmiI GTMKAC 1 cut(s) 330
XspI CTAG 5 cut(s) 317, 555, 1098, 2217, 2876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.