pycom461g00030

ATP synthase subunit alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000461
OG0009874
Physical Location & Seq
Reverse (-)
20286 .. 22268
1983 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom461g00030.1

Sequence Viewer

Length: 1983 bp
ATGGAATTCTCTACCAGAGCTGCGGAACTAACGACTCTATTAGAAAGTAGAATTACCAACTTTTACACGAATTTTCAAGTGGATGAGATCGGTCGAGTGGTCTCCGTTGGAGATGGGATTGCGCGTGTTTATGGATTGAACCAGATTCAAGCTGGGGAAATGGTTGAATTTGCCGGCGGTGTGAAAGGAATAGCGTTGAATCTTGAGAATGAGAATGTAGGGATTGTTGTCTTTGGTAGTGATACCGCTATTAAAGAAGGAGATCTTGTCAAGCGCACTGGATCCATTGTGGATGTTCCTGCGGGAAAGGCTATGCTAGGGCGTGTGGTCGACGGGTTGGGAGTTCCTATTGATGGAAGAGGGGCTCTAAGCGATCACGAGCGAAGACGTGTCGAAGTGAAAGCCCCTGGGATTATTGAACGTAAATCTGTGCACGAGCCTATGCAAACAGGGTTAAAAGCGGTAGATAGCCTGGTTCCTGTAGGCCGTGGTCAACGAGAACTTATAATCGGGGACCGACAAACTGGAAAAACAGCTATTGCTATCGATACCATATTAAACCAAAAGCAACTGAACTCAAGGGCCACCTCTGAGAGTGAGACATTGTATTGTGTCTATGTAGCAATTGGACAGAAACGCTCAACTGTGGCACAATTGGTTCAAATTCTTTCAGAAGCGAATGCTTTGGAATATTCCATTCTTGTAGCAGCCACCGCTTCGGATCCAGCTCCTCTGCAATTTCTGGCCCCATATTCTGGGTGTGCCATGGGGGAATATTTCCGCGATAATGGAATGCACGCATTAATAATCTATGATGATCTTAGTAAACAGGCGGTGGCATATCGACAAATGTCATTATTGTTACGACGACCGCCAGGCCGTGAGGCTTTCCCAGGCGATGTTTTCTATTTGCATTCCCGTCTCTTAGAAAGAGCCGCTAAACGATCGGACCAGACAGGTGCAGGTAGCTTGACCGCCTTACCCGTCATTGAAACACAAGCTGGAGACGTATCGGCCTATATTCCAACCAATGTGATCTCCATTACCGATGGACAAATCTGTTCGGAAACAGAACTCTTTTATCGCGGAATTAGACCTGCTATTAACGTGGGCTTATCTGTCAGTCGCGTCGGGTCTGCCGCTCAGTTAAAAATTATGAAACAAGTCTGCGGTAGTTCAAAACTGGAATTGGCACAATATCGCGAAGTGGCCGCCCTTGCTCAATTTGGGTCAGACCTTGATGCCGCGACTCAGGCATTACTCAATAGAGGTGCAAGGCTTACAGAAGTACCGAAACAACCACAATATGCACCACTTCCAATTGAAAAACAAATTATAGTCATTTATGCAGCTGTCAATGGATTCTGTGATCGAATGCCACTGGACAGAATCTCTAAATATGAGAGAGCCTTTCCAATTATAGTAAGGCAGGTAATCAAAATTGTGATAAAAAATTTTTTGAACGCCGCTTTATTCTATCTCTTCGCTTTGGTTTTTGGCTCGTTAGAATTTGTAGGATTATTTATGGATCCTATCGCGCCAAACGCCGGCATAGCCGAGGAAATAGTTGCTGACATGGGAACGGTGGAAGACGCTCACGACGAGAGGGACGGACCAAACTTTGACCGGCTGCGGTATGAAATACAACAGTACAGCGAGGCGAGCAGGGAGTGCTCACGCCTTGCTGTAGATTCCGTGGAACGAGCGGAAAATTTGGTGGACGAGGTCAACTATCGGGTATCCTTGCCGGATCAGGGGCCGGAGTGGAGGGAACACACAGCCCAATTGATTTCGGAGGCTGAAGACGAAGTCAACTACCCCGCAGGTGACCTGGAGATGGCTCAAGAACTGGACCAAGACAAGAAAGAAGCACAAGATAAATATAATAGAGAACTAGACGCAGAATATAGTCGCCTACGGGATCGAGAGAAGGCCCTACAAGAAGATAATGACCGCCTAAAAAGACTTTTAAACCGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko05010 ko05012 ko05016 map00190 map01100 map04714 map05010 map05012 map05016
Pfam Domains
Protein Families
ATP-synt_ab ATP-synt_ab_C ATP-synt_ab_N

Protein Analysis

661

Amino Acids

72.77

Weight (kDa)

5.07

Isoelectric Point (pI)

33.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_ab_N PF02874 27 - 94 5.4e-19 ATP synthase alpha/beta family, beta-barrel domain
ATP-synt_ab PF00006 151 - 375 1.6e-77 ATP synthase alpha/beta family, nucleotide-binding domain
ATP-synt_ab_C PF00306 382 - 478 2e-35 ATP synthase alpha/beta chain, C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 506
AarI CACCTGC 1 cut(s) 1814
Acc36I ACCTGC 4 cut(s) 953, 1105, 1420, 1814
AccB7I CCANNNNNTGG 1 cut(s) 755
AccBSI CCGCTC 2 cut(s) 1142, 1706
AccI GTMKAC 1 cut(s) 330
AccII CGCG 7 cut(s) 124, 783, 1086, 1128, 1203, 1247, 1538
AclWI GGATC 8 cut(s) 276, 289, 716, 729, 1523, 1536, 1758, 1929
AcoI YGGCCR 1 cut(s) 1209
AcsI RAATTY 7 cut(s) 5, 70, 167, 663, 1453, 1508, 1711
AcuI CTGAAG 1 cut(s) 1821
AfaI GTAC 2 cut(s) 1290, 1652
AfiI CCNNNNNNNGG 5 cut(s) 353, 755, 1547, 1754, 1837
AflIII ACRYGT 1 cut(s) 388
AgeI ACCGGT 1 cut(s) 1974
AjiI CACGTC 1 cut(s) 389
AjnI CCWGG 5 cut(s) 406, 471, 874, 892, 1830
AluBI AGCT 7 cut(s) 20, 152, 536, 728, 969, 1001, 1352
AluI AGCT 7 cut(s) 20, 152, 536, 728, 969, 1001, 1352
Alw21I GWGCWC 2 cut(s) 435, 1676
Alw26I GTCTC 4 cut(s) 106, 593, 926, 999
Alw44I GTGCAC 1 cut(s) 431
AlwI GGATC 8 cut(s) 276, 289, 716, 729, 1523, 1536, 1758, 1929
AoxI GGCC 8 cut(s) 484, 582, 744, 877, 1014, 1209, 1757, 1932
ApaLI GTGCAC 1 cut(s) 431
ApeKI GCWGC 4 cut(s) 20, 707, 1349, 1630
ApoI RAATTY 7 cut(s) 5, 70, 167, 663, 1453, 1508, 1711
AseI ATTAAT 1 cut(s) 803
AsiGI ACCGGT 1 cut(s) 1974
AspLEI GCGC 3 cut(s) 124, 276, 1540
AspS9I GGNCC 8 cut(s) 514, 582, 745, 949, 1613, 1757, 1852, 1933
AsuHPI GGTGA 1 cut(s) 1838
AvaII GGWCC 4 cut(s) 514, 949, 1613, 1852
BaeGI GKGCMC 1 cut(s) 435
BaeI ACNNNNGTAYC 2 cut(s) 1272, 1305
BamHI GGATCC 3 cut(s) 281, 721, 1528
BanII GRGCYC 1 cut(s) 367
BauI CACGAG 2 cut(s) 377, 434
BbsI GAAGAC 3 cut(s) 391, 1596, 1809
Bbv12I GWGCWC 2 cut(s) 435, 1676
BbvI GCAGC 4 cut(s) 7, 719, 1361, 1617
BccI CCATC 4 cut(s) 107, 347, 1043, 1831
BceAI ACGGC 2 cut(s) 471, 864
BciT130I CCWGG 5 cut(s) 408, 473, 876, 894, 1832
BciVI GTATCC 1 cut(s) 1750
BcoDI GTCTC 4 cut(s) 106, 593, 926, 999
BfaI CTAG 2 cut(s) 317, 1895
BfmI CTRYAG 2 cut(s) 480, 1686
BfuAI ACCTGC 4 cut(s) 953, 1105, 1420, 1814
BfuI GTATCC 1 cut(s) 1750
BglII AGATCT 1 cut(s) 262
BisI GCNGC 9 cut(s) 21, 708, 936, 1140, 1212, 1245, 1350, 1467, 1631
BlsI GCNGC 9 cut(s) 22, 709, 937, 1141, 1213, 1246, 1351, 1468, 1632
Bme1390I CCNGG 5 cut(s) 408, 473, 876, 894, 1832
Bme18I GGWCC 4 cut(s) 514, 949, 1613, 1852
BmgBI CACGTC 1 cut(s) 389
BmgT120I GGNCC 8 cut(s) 514, 582, 745, 949, 1613, 1757, 1852, 1933
BmiI GGNNCC 7 cut(s) 283, 477, 515, 723, 747, 1530, 1758
BmrFI CCNGG 5 cut(s) 408, 473, 876, 894, 1832
BmsI GCATC 1 cut(s) 1231
BoxI GACNNNNGTC 1 cut(s) 850
BpiI GAAGAC 3 cut(s) 391, 1596, 1809
BpmI CTGGAG 2 cut(s) 1023, 1853
BpuEI CTTGAG 3 cut(s) 224, 562, 1827
Bsa29I ATCGAT 1 cut(s) 546
BsaI GGTCTC 1 cut(s) 106
BsaJI CCNNGG 7 cut(s) 406, 407, 487, 765, 892, 1557, 1695
BsaWI WCCGGW 1 cut(s) 1974
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 5 cut(s) 353, 755, 1547, 1754, 1837
Bse118I RCCGGY 4 cut(s) 173, 1547, 1626, 1974
Bse1I ACTGG 5 cut(s) 283, 529, 1188, 1386, 1854
BseBI CCWGG 5 cut(s) 408, 473, 876, 894, 1832
BseCI ATCGAT 1 cut(s) 546
BseDI CCNNGG 7 cut(s) 406, 407, 487, 765, 892, 1557, 1695
BseGI GGATG 2 cut(s) 88, 298
BseLI CCNNNNNNNGG 5 cut(s) 353, 755, 1547, 1754, 1837
BseMII CTCAG 3 cut(s) 582, 1157, 1265
BseNI ACTGG 5 cut(s) 283, 529, 1188, 1386, 1854
BseRI GAGGAG 1 cut(s) 720
BseSI GKGCMC 1 cut(s) 435
BseXI GCAGC 4 cut(s) 7, 719, 1361, 1617
BseYI CCCAGC 1 cut(s) 152
BsgI GTGCAG 1 cut(s) 981
Bsh1236I CGCG 7 cut(s) 124, 783, 1086, 1128, 1203, 1247, 1538
Bsh1285I CGRYCG 3 cut(s) 94, 872, 947
BshFI GGCC 8 cut(s) 486, 584, 746, 879, 1016, 1211, 1759, 1934
BshTI ACCGGT 1 cut(s) 1974
BshVI ATCGAT 1 cut(s) 546
BsiEI CGRYCG 3 cut(s) 94, 872, 947
BsiHKAI GWGCWC 2 cut(s) 435, 1676
BsiSI CCGG 6 cut(s) 174, 1548, 1627, 1748, 1760, 1975
BslFI GGGAC 2 cut(s) 527, 1622
BslI CCNNNNNNNGG 5 cut(s) 353, 755, 1547, 1754, 1837
BsmAI GTCTC 4 cut(s) 106, 593, 926, 999
BsmBI CGTCTC 2 cut(s) 926, 999
BsmFI GGGAC 2 cut(s) 527, 1622
BsmI GAATGC 4 cut(s) 685, 798, 913, 1380
BsnI GGCC 8 cut(s) 486, 584, 746, 879, 1016, 1211, 1759, 1934
Bso31I GGTCTC 1 cut(s) 106
Bsp1286I GDGCHC 3 cut(s) 367, 435, 1676
Bsp19I CCATGG 1 cut(s) 765
Bsp68I TCGCGA 1 cut(s) 1203
BspANI GGCC 8 cut(s) 486, 584, 746, 879, 1016, 1211, 1759, 1934
BspCNI CTCAG 3 cut(s) 583, 1156, 1264
BspDI ATCGAT 1 cut(s) 546
BspFNI CGCG 7 cut(s) 124, 783, 1086, 1128, 1203, 1247, 1538
BspLI GGNNCC 7 cut(s) 283, 477, 515, 723, 747, 1530, 1758
BspMI ACCTGC 4 cut(s) 953, 1105, 1420, 1814
BspPI GGATC 8 cut(s) 276, 289, 716, 729, 1523, 1536, 1758, 1929
BspTNI GGTCTC 1 cut(s) 106
BsrBI CCGCTC 2 cut(s) 1142, 1706
BsrFI RCCGGY 4 cut(s) 173, 1547, 1626, 1974
BsrI ACTGG 5 cut(s) 283, 529, 1188, 1386, 1854
BssAI RCCGGY 4 cut(s) 173, 1547, 1626, 1974
BssECI CCNNGG 7 cut(s) 406, 407, 487, 765, 892, 1557, 1695
BssSI CACGAG 2 cut(s) 377, 434
BssT1I CCWWGG 1 cut(s) 765
Bst2BI CACGAG 2 cut(s) 377, 434
Bst2UI CCWGG 5 cut(s) 408, 473, 876, 894, 1832
Bst4CI ACNGT 3 cut(s) 646, 1585, 1650
Bst6I CTCTTC 2 cut(s) 352, 1487
BstAPI GCANNNNNTGC 1 cut(s) 1671
BstC8I GCNNGC 4 cut(s) 175, 798, 1549, 1663
BstDEI CTNAG 6 cut(s) 368, 591, 821, 925, 1143, 1251
BstDSI CCRYGG 3 cut(s) 487, 765, 1695
BstEII GGTNACC 1 cut(s) 1826
BstF5I GGATG 2 cut(s) 88, 298
BstFNI CGCG 7 cut(s) 124, 783, 1086, 1128, 1203, 1247, 1538
BstHHI GCGC 3 cut(s) 124, 276, 1540
BstMAI GTCTC 4 cut(s) 106, 593, 926, 999
BstMCI CGRYCG 3 cut(s) 94, 872, 947
BstMWI GCNNNNNNNGC 8 cut(s) 308, 713, 1217, 1253, 1544, 1553, 1662, 1671
BstNI CCWGG 5 cut(s) 408, 473, 876, 894, 1832
BstPAI GACNNNNGTC 1 cut(s) 850
BstPI GGTNACC 1 cut(s) 1826
BstSCI CCNGG 5 cut(s) 406, 471, 874, 892, 1830
BstSFI CTRYAG 2 cut(s) 480, 1686
BstSLI GKGCMC 1 cut(s) 435
BstUI CGCG 7 cut(s) 124, 783, 1086, 1128, 1203, 1247, 1538
BstV1I GCAGC 4 cut(s) 7, 719, 1361, 1617
BstV2I GAAGAC 3 cut(s) 391, 1596, 1809
BstX2I RGATCY 4 cut(s) 262, 281, 721, 1528
BstYI RGATCY 4 cut(s) 262, 281, 721, 1528
Bsu15I ATCGAT 1 cut(s) 546
BsuI GTATCC 1 cut(s) 1750
BsuRI GGCC 8 cut(s) 486, 584, 746, 879, 1016, 1211, 1759, 1934
BsuTUI ATCGAT 1 cut(s) 546
BtgI CCRYGG 3 cut(s) 487, 765, 1695
BtgZI GCGATG 1 cut(s) 912
BtrI CACGTC 1 cut(s) 389
BtsCI GGATG 2 cut(s) 88, 298
BtsIMutI CAGTG 2 cut(s) 276, 1379
BtuMI TCGCGA 1 cut(s) 1203
BveI ACCTGC 4 cut(s) 953, 1105, 1420, 1814
Cac8I GCNNGC 4 cut(s) 175, 798, 1549, 1663
CfoI GCGC 3 cut(s) 124, 276, 1540
Cfr10I RCCGGY 4 cut(s) 173, 1547, 1626, 1974
Cfr13I GGNCC 8 cut(s) 514, 582, 745, 949, 1613, 1757, 1852, 1933
ClaI ATCGAT 1 cut(s) 546
CseI GACGC 3 cut(s) 1117, 1601, 1907
Csp6I GTAC 2 cut(s) 1289, 1651
CspAI ACCGGT 1 cut(s) 1974
CviAII CATG 2 cut(s) 766, 1576
CviQI GTAC 2 cut(s) 1289, 1651
DdeI CTNAG 6 cut(s) 368, 591, 821, 925, 1143, 1251
DraI TTTAAA 1 cut(s) 1971
EaeI YGGCCR 1 cut(s) 1209
Eam1104I CTCTTC 2 cut(s) 352, 1487
EarI CTCTTC 2 cut(s) 352, 1487
Eco130I CCWWGG 1 cut(s) 765
Eco24I GRGCYC 1 cut(s) 367
Eco31I GGTCTC 1 cut(s) 106
Eco47I GGWCC 4 cut(s) 514, 949, 1613, 1852
Eco57I CTGAAG 1 cut(s) 1821
Eco91I GGTNACC 1 cut(s) 1826
EcoO109I RGGNCCY 1 cut(s) 1933
EcoO65I GGTNACC 1 cut(s) 1826
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 5 cut(s) 406, 471, 874, 892, 1830
EcoT14I CCWWGG 1 cut(s) 765
EcoT38I GRGCYC 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 765
Esp3I CGTCTC 2 cut(s) 926, 999
FaeI CATG 2 cut(s) 769, 1579
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FaqI GGGAC 2 cut(s) 527, 1622
FatI CATG 2 cut(s) 765, 1575
FauI CCCGC 2 cut(s) 295, 1828
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 9 cut(s) 21, 708, 936, 1140, 1212, 1245, 1350, 1467, 1631
FokI GGATG 2 cut(s) 95, 305
FriOI GRGCYC 1 cut(s) 367
Fsp4HI GCNGC 9 cut(s) 21, 708, 936, 1140, 1212, 1245, 1350, 1467, 1631
FspBI CTAG 2 cut(s) 317, 1895
GlaI GCGC 3 cut(s) 123, 275, 1539
GluI GCNGC 9 cut(s) 21, 708, 936, 1140, 1212, 1245, 1350, 1467, 1631
GsaI CCCAGC 1 cut(s) 156
GsuI CTGGAG 2 cut(s) 1023, 1853
HaeIII GGCC 8 cut(s) 486, 584, 746, 879, 1016, 1211, 1759, 1934
HapII CCGG 6 cut(s) 174, 1548, 1627, 1748, 1760, 1975
HgaI GACGC 3 cut(s) 1117, 1601, 1907
HhaI GCGC 3 cut(s) 124, 276, 1540
Hin1II CATG 2 cut(s) 769, 1579
Hin6I GCGC 3 cut(s) 122, 274, 1538
HinP1I GCGC 3 cut(s) 122, 274, 1538
HincII GTYRAC 4 cut(s) 331, 494, 1729, 1813
HindII GTYRAC 4 cut(s) 331, 494, 1729, 1813
HinfI GANTC 7 cut(s) 34, 145, 199, 1249, 1362, 1389, 1691
HpaII CCGG 6 cut(s) 174, 1548, 1627, 1748, 1760, 1975
HphI GGTGA 1 cut(s) 1838
Hpy166II GTNNAC 7 cut(s) 331, 433, 494, 827, 1720, 1729, 1813
Hpy188I TCNGA 7 cut(s) 592, 673, 721, 949, 1066, 1234, 1795
Hpy188III TCNNGA 6 cut(s) 203, 377, 1202, 1598, 1844, 1925
Hpy8I GTNNAC 7 cut(s) 331, 433, 494, 827, 1720, 1729, 1813
Hpy99I CGWCG 4 cut(s) 335, 870, 1133, 1604
HpyAV CCTTC 2 cut(s) 251, 1924
HpyCH4III ACNGT 3 cut(s) 646, 1585, 1650
HpyCH4IV ACGT 4 cut(s) 388, 421, 1008, 1107
HpyCH4V TGCA 9 cut(s) 433, 445, 736, 796, 913, 962, 1274, 1310, 1349
HpyF10VI GCNNNNNNNGC 8 cut(s) 308, 713, 1217, 1253, 1544, 1553, 1662, 1671
HpyF3I CTNAG 6 cut(s) 368, 591, 821, 925, 1143, 1251
HpySE526I ACGT 4 cut(s) 388, 421, 1008, 1107
Hsp92II CATG 2 cut(s) 769, 1579
HspAI GCGC 3 cut(s) 122, 274, 1538
KroI GCCGGC 2 cut(s) 173, 1547
KroNI GCCGGC 2 cut(s) 175, 1549
LmnI GCTCC 1 cut(s) 733
Lsp1109I GCAGC 4 cut(s) 7, 719, 1361, 1617
LweI GCATC 1 cut(s) 1231
MaeI CTAG 2 cut(s) 317, 1895
MaeII ACGT 4 cut(s) 388, 421, 1008, 1107
MaeIII GTNAC 2 cut(s) 861, 1826
MbiI CCGCTC 2 cut(s) 1142, 1706
MboII GAAGA 6 cut(s) 369, 396, 1474, 1601, 1814, 1955
MfeI CAATTG 4 cut(s) 624, 653, 1320, 1784
MflI RGATCY 4 cut(s) 262, 281, 721, 1528
MhlI GDGCHC 3 cut(s) 367, 435, 1676
MlyI GAGTC 2 cut(s) 28, 1243
MmeI TCCRAC 2 cut(s) 88, 1049
MroNI GCCGGC 2 cut(s) 173, 1547
MseI TTAA 7 cut(s) 252, 455, 557, 803, 1104, 1148, 1970
MspA1I CMGCKG 1 cut(s) 1352
MspI CCGG 6 cut(s) 174, 1548, 1627, 1748, 1760, 1975
MspR9I CCNGG 5 cut(s) 408, 473, 876, 894, 1832
MunI CAATTG 4 cut(s) 624, 653, 1320, 1784
Mva1269I GAATGC 4 cut(s) 685, 798, 913, 1380
MvaI CCWGG 5 cut(s) 408, 473, 876, 894, 1832
MvnI CGCG 7 cut(s) 124, 783, 1086, 1128, 1203, 1247, 1538
MwoI GCNNNNNNNGC 8 cut(s) 308, 713, 1217, 1253, 1544, 1553, 1662, 1671
NaeI GCCGGC 2 cut(s) 175, 1549
NcoI CCATGG 1 cut(s) 765
NgoMIV GCCGGC 2 cut(s) 173, 1547
NlaIII CATG 2 cut(s) 769, 1579
NlaIV GGNNCC 7 cut(s) 283, 477, 515, 723, 747, 1530, 1758
NmeAIII GCCGAG 1 cut(s) 1582
NmuCI GTSAC 1 cut(s) 1826
NruI TCGCGA 1 cut(s) 1203
PaqCI CACCTGC 1 cut(s) 1814
PasI CCCWGGG 1 cut(s) 407
PcsI WCGNNNNNNNCGW 1 cut(s) 1599
PctI GAATGC 4 cut(s) 685, 798, 913, 1380
PdiI GCCGGC 2 cut(s) 175, 1549
PfeI GAWTC 5 cut(s) 145, 199, 1362, 1389, 1691
PflFI GACNNNGTC 2 cut(s) 1724, 1808
PflMI CCANNNNNTGG 1 cut(s) 755
PinAI ACCGGT 1 cut(s) 1974
PkrI GCNGC 9 cut(s) 22, 709, 937, 1141, 1213, 1246, 1351, 1468, 1632
Ple19I CGATCG 1 cut(s) 947
PleI GAGTC 2 cut(s) 28, 1243
PpsI GAGTC 2 cut(s) 28, 1243
PshAI GACNNNNGTC 1 cut(s) 850
PshBI ATTAAT 1 cut(s) 803
PsiI TTATAA 1 cut(s) 506
Psp6I CCWGG 5 cut(s) 406, 471, 874, 892, 1830
PspEI GGTNACC 1 cut(s) 1826
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 5 cut(s) 406, 471, 874, 892, 1830
PspN4I GGNNCC 7 cut(s) 283, 477, 515, 723, 747, 1530, 1758
PspPI GGNCC 8 cut(s) 514, 582, 745, 949, 1613, 1757, 1852, 1933
PsuI RGATCY 4 cut(s) 262, 281, 721, 1528
PsyI GACNNNGTC 2 cut(s) 1724, 1808
PvuI CGATCG 1 cut(s) 947
PvuII CAGCTG 1 cut(s) 1352
RruI TCGCGA 1 cut(s) 1203
RsaI GTAC 2 cut(s) 1290, 1652
RsaNI GTAC 2 cut(s) 1289, 1651
SalI GTCGAC 1 cut(s) 329
SaqAI TTAA 7 cut(s) 252, 455, 557, 803, 1104, 1148, 1970
SatI GCNGC 9 cut(s) 21, 708, 936, 1140, 1212, 1245, 1350, 1467, 1631
Sau96I GGNCC 8 cut(s) 514, 582, 745, 949, 1613, 1757, 1852, 1933
SchI GAGTC 2 cut(s) 28, 1243
ScrFI CCNGG 5 cut(s) 408, 473, 876, 894, 1832
SduI GDGCHC 3 cut(s) 367, 435, 1676
SfaNI GCATC 1 cut(s) 1231
SfcI CTRYAG 2 cut(s) 480, 1686
SinI GGWCC 4 cut(s) 514, 949, 1613, 1852
SmlI CTYRAG 3 cut(s) 203, 577, 1842
SmoI CTYRAG 3 cut(s) 203, 577, 1842
SspI AATATT 2 cut(s) 692, 776
SspMI CTAG 2 cut(s) 317, 1895
StyD4I CCNGG 5 cut(s) 406, 471, 874, 892, 1830
StyI CCWWGG 1 cut(s) 765
TaaI ACNGT 3 cut(s) 646, 1585, 1650
TaiI ACGT 4 cut(s) 391, 424, 1011, 1110
TaqI TCGA 7 cut(s) 94, 330, 393, 546, 844, 1372, 1924
TaqII GACCGA 2 cut(s) 80, 531
TatI WGTACW 1 cut(s) 1650
TauI GCSGC 5 cut(s) 938, 1142, 1214, 1247, 1469
TfiI GAWTC 5 cut(s) 145, 199, 1362, 1389, 1691
Tru1I TTAA 7 cut(s) 252, 455, 557, 803, 1104, 1148, 1970
Tru9I TTAA 7 cut(s) 252, 455, 557, 803, 1104, 1148, 1970
TscAI CASTG 2 cut(s) 283, 1386
TseFI GTSAC 1 cut(s) 1826
TseI GCWGC 4 cut(s) 20, 707, 1349, 1630
Tsp45I GTSAC 1 cut(s) 1826
TspDTI ATGAA 2 cut(s) 1172, 1653
TspGWI ACGGA 3 cut(s) 94, 1626, 1684
TspRI CASTG 2 cut(s) 283, 1386
Tth111I GACNNNGTC 2 cut(s) 1724, 1808
Van91I CCANNNNNTGG 1 cut(s) 755
VneI GTGCAC 1 cut(s) 431
VpaK11BI GGWCC 4 cut(s) 514, 949, 1613, 1852
VspI ATTAAT 1 cut(s) 803
XapI RAATTY 7 cut(s) 5, 70, 167, 663, 1453, 1508, 1711
XcmI CCANNNNNNNNNTGG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 330
XspI CTAG 2 cut(s) 317, 1895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.