Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0011804

Physical Location & Seq

Genomic Coordinates Forward (+)

22485969 .. 22491771

Gene Structure 5803 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g28820.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3201 bp

ATGGCGGGAAATGACTGGGTGAACGGTTACTTAGAGGCCATTCTCGACGTCGGTCCCGGCCTCGACGACGCCAAATCCTCGTCGTCTTTGCTGCTCCGGGAACGCGGTCGTTTCAGTCCCACACGCTACTTCGTTGAGGAGGTCATCACTCGCTACGATGAGACTGATCTCCACCGCTCCTGGGTTCGGGCTGCCGCGACGGCGAGGAGCGCCGAGGAGAGGAACACCAGGCTCGAGAACATGTGCTGGCGGATTTGGAATCTCGCTCGCAAAAAGAAACAGCTTGAGGGAGAAGAAGCTCAACGAACAGCTAGCCGTCGGCTGGAACGTGAAATGGGAAGGAGGGAGGCAACTGAGGATATGTCTGAGGACTTATCTGAAGGAGAGAGTAGAGATGCGGTTGGTGATATTTTGGCTCATAGTGATAGCTTTAGAGGAGGCAGAATGCGTAGAGTGAATTCTATGGATGTGATGGACAATGTGATGGAGAACTGGGCTGCTCATCAAAAGGAAAAGAAATTCTATATAGTGCTGATAAGCCTCCATGGCTTGATACGCGGTGAAAACATGGAGCTTGGTCGTGATTCTGATACAGGTGGCCAGGTCAAGTATGTTGTGGAACTTGCCAGGGCCTTGGGTTCAATGCCTGGAGTTTATCGTGTTGATTTACTAACAAGACAAGTTTCAGCTCCAGATGTTGATTGGAGTTATGGGGAACCAACTGAAATGTTGAATCCAGTGAACTCTGATAATCCACAAGAGGAGCTTGGGGAGAGTAGTGGGGCTTATATAGTGCGTATACCATTTGGTCCAAAAGACAAATACATTCCAAAAGAACTCCTTTGGCCTCACATTCCTGAGTTTGTAGACGGAGCACTTAACCATATTATACAGTTGTCGAAAGCGCTTGGAGAGCAAATCGGAGGTGGGGAACAAGTGTGGCCAGTGGCAATTCATGGACACTATGCAGATGCAGGTGACTCTGCTGCTCTTCTTTCAGGAGCCTTGAATGTCCCAATGTTATTTACGGGTCACTCACTTGGACGAGATAAGCTTGAGCAACTTCTGAAACAAGGACGCCAATCAAGAGAAGAAATAAATACAACATACAAAATTATGAGAAGGATAGAGGCAGAGGAACTATCACTTGACTCCTCTGAGATTGTTATAACCAGCACCAGACAGGAGATAGACTCACAATGGAACTTGTATGATGGCTTTGATCCAATACTAGAACGTAAACTGAGAGCAAGAATCAAAAGGGGTGTTAGCTGTCATGGCAGGTTTATGCCTCGCACGGTTGTAATACCTCCTGGAATGGAATTTCATCATATTATTCCACCTGCAGATGGTGATGCAGATGGGGAAGGAGAAAGAAATGATGATAGTTCTGCCAATCCTGACCTGCCAATTTGGTCAGAGATTATGCGTTTCTTTACCAACCCACGCAAGCCTATGATACTTCTTCTTGCCAGGGCAGATCCTAAGAAGAATATTATGACTTTAGTTAAAGCGTTTGGAGAGTGTCGCCCATTGAGGGATCTTGCTAACCTTACGTTAATAATGGGAAACCGTGACGATATTGATGAAATGTCCAGCACAAATGCCTCCGTGCTTCTTTCCATTTTAAAGCTGATTGACAGGTATGATCTTTATGGTCATGTGGCATATCCCAAACACCACAAGCAGTCTGATGTTCCTGACATCTATCGTCTAGCAGCAAAAACAAAGGGTGTTTTCATCAATCCAGCTTTCATTGAGCCATTTGGGCTTACTTTAATAGAGGCAGCAGCTCATGGCTTACCTATTGTTGCCACAAAAAATGGGGGTCCTGTTGATATACATCGGGTTCTTGATAATGGTCTGCTAATTGACCCCCATGACCAGCAGTCAATTGCTGATGCCCTACTGAACCTTGTTTCGGATAAGCATCTTTGGGCAAGATGCAGGCACAATGGACTGAAAAACATACACCTTTTCTCATGGCCAGAGCATTGTAAGACCTACCTAACTCGCATAACCAGTTGCAAACCAAGGCAGCCACAATGGCGAAGAACTGCTGCTGAATTTGATTCTTCAGGATCAGATTCATCACCTGGTGATTCCTTAAGGGATATAAAAGATATATCTTTGAACTTGAAGCTATCTATGTCTATGGATGGTGAGAAACTTGAAGGGGCTGGATCTCTTGATAATGCTTTAGAGAATGATGCCGCTGGAGGAAAAGTTAAAGAAGAGAATGTGGATGTGACAATGTCAAAGGGTGCTCTAGAAGGCAGTCAAAAGGCTGGTGCCACAGATAGAGAAGACAATGCTGGGGCTGGTAAATTTCCTGTGCTGAGGAAAAGGAAGCATGTATTTGTTATTGCTGTGGATTGTGATACAACCTCAGAGTTCTGTGAAATTTTTGAAAAGGTTATTGAGACAGTAACGAAGGAAAAGTATGCAGGACCCATAGGATTCATAATGGCAACATCTTATTCCATATCAGAGATACAGTCTCTTTTGGTCACAGGAGGTTTGAGCCCATCACAGTTCGATGGTTTTATATGCAGCAGTGGTGGTGAGCTCTACTATCCATCTTCAAGCTGCGAGGATAGTCCTTCTGGACTTCCCTTTGTGGTAGACTTGGATTATCGTTCACACACTGAATACCGTTGGGGTGGAGAAGGTTTGAGAAAGACTTTGGTTCGTTGGGTCGCTTCTTTCAATGAGAAAAAGGGAGATGGACAAGTTGCAGCAGATGAATCAGGAACAACTAAGCATTGCTATGCATTTACTGTGAAAGATCCAGCATCGATTTACCCCATTAAGGAACTCCGGAAAATTTTGAGAATTCAGGCACTTCGATGCAATGTTGTATATTCTCAGAATGGTACGAGGCTTAAGGTCATCCCTGTATTGGCTTCTAGATCACAAGCCCTCAGGTATCTGTATGTCCGTTGGGGATTGGACTTGTCAAACATTGTTGCCTTTGTGGGAGAACTAGGCGACACAGACTACGAAGGCTTGCTTGGTGGTGTACACAAAACTGTTATATTGAAGGGAGTAGGTACCAGTGCTCGTAAGCTTCATGCTAACCGAAACTTTCCTTTGGAACATGTCCTTCCAGTTGACAGTCCCAATGTTGTTCAAAGTGAAGGTTGCTCTGATGAAGACATTCGATCATCACTGGTGAAACTAAAGATCATCAAGGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0008150 GO:0008194 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010927 GO:0016020 GO:0016043 GO:0016740 GO:0016757 GO:0016758 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0035251 GO:0043062 GO:0044085 GO:0044464 GO:0045229 GO:0046524 GO:0046527 GO:0046903 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0071836 GO:0071840 GO:0071944 GO:0085029

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 S6PP Sucrose_synth

Protein Analysis

1067

Amino Acids

118.69

Weight (kDa)

5.94

Isoelectric Point (pI)

45.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GT-B_Sucrose_synth	PF00862	173 - 441	2.4e-10	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	481 - 654	9e-22	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	485 - 642	2.6e-15	Glycosyl transferases group 1
S6PP	PF05116	808 - 999	6.2e-11	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011804)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11110
fragaria_vesca	FvH4_2g28820 FvH4_2g28820
malus_domestica	MD08G1157000.v1.1 MD15G1127900.v1.1
prunus_persica	Prupe.1G483200_v2.0.a1 Prupe.1G483200_v2.0.a1
pyrus_communis	pycom08g12930 pycom15g11500
rosa_chinensis	RchiOBHm_Chr6g0297571
rosa_laevigata	RLG00000011582
rosa_roxburghii	Rroxscaffold_7G00170350
rosa_rugosa	Rorug06G0273700 Rorug06G0273800 Rorug06G0273800
rosa_samantha	Rh6BG392800 Rh6CG398600 Rh6DG385200
rosa_wichuraiana	Rw6G033510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1169
AarI	CACCTGC	2 cut(s)	965, 1351
AatII	GACGTC	1 cut(s)	51
Acc36I	ACCTGC	4 cut(s)	965, 1272, 1351, 1413
Acc65I	GGTACC	1 cut(s)	3053
AccB1I	GGYRCC	2 cut(s)	2291, 3053
AccBSI	CCGCTC	1 cut(s)	177
AccI	GTMKAC	3 cut(s)	799, 867, 2625
AccII	CGCG	3 cut(s)	105, 197, 558
AccIII	TCCGGA	1 cut(s)	2820
AciI	CCGC	8 cut(s)	5, 105, 175, 195, 250, 398, 558, 2214
AclWI	GGATC	6 cut(s)	1217, 1475, 1548, 2089, 2191, 2783
AcoI	YGGCCR	3 cut(s)	598, 941, 1985
AcsI	RAATTY	8 cut(s)	457, 518, 1322, 2066, 2327, 2403, 2826, 2835
AcuI	CTGAAG	2 cut(s)	399, 2061
AcyI	GRCGYC	3 cut(s)	48, 69, 1078
AdeI	CACNNNGTG	1 cut(s)	2099
AfaI	GTAC	3 cut(s)	2878, 3024, 3055
AfeI	AGCGCT	1 cut(s)	906
AfiI	CCNNNNNNNGG	9 cut(s)	181, 186, 219, 322, 1349, 1537, 1921, 2812, 2902
AflII	CTTAAG	2 cut(s)	2107, 2885
AflIII	ACRYGT	2 cut(s)	240, 3100
AhdI	GACNNNNNGTC	1 cut(s)	1888
AjnI	CCWGG	8 cut(s)	179, 227, 600, 626, 646, 1312, 1472, 2095
AjuI	GAANNNNNNNTTGG	2 cut(s)	2997, 3029
Alw21I	GWGCWC	4 cut(s)	877, 2269, 2571, 3064
Alw26I	GTCTC	3 cut(s)	155, 2417, 2505
AlwI	GGATC	6 cut(s)	1217, 1475, 1548, 2089, 2191, 2783
Ama87I	CYCGRG	1 cut(s)	233
Aor13HI	TCCGGA	1 cut(s)	2820
Aor51HI	AGCGCT	1 cut(s)	906
AoxI	GGCC	7 cut(s)	36, 58, 598, 630, 845, 941, 1985
ApoI	RAATTY	8 cut(s)	457, 518, 1322, 2066, 2327, 2403, 2826, 2835
Asp700I	GAANNNNTTC	1 cut(s)	3159
Asp718I	GGTACC	1 cut(s)	3053
AspLEI	GCGC	2 cut(s)	212, 907
AspS9I	GGNCC	5 cut(s)	53, 630, 809, 1829, 2450
AsuC2I	CCSGG	2 cut(s)	57, 98
AsuNHI	GCTAGC	1 cut(s)	311
AvaI	CYCGRG	1 cut(s)	233
AvaII	GGWCC	4 cut(s)	53, 809, 1829, 2450
AxyI	CCTNAGG	1 cut(s)	2924
BalI	TGGCCA	3 cut(s)	600, 943, 1987
BanI	GGYRCC	2 cut(s)	2291, 3053
BanII	GRGCYC	2 cut(s)	2528, 2571
BbsI	GAAGAC	2 cut(s)	2313, 3162
Bbv12I	GWGCWC	4 cut(s)	877, 2269, 2571, 3064
BbvCI	CCTCAGC	1 cut(s)	2339
BceAI	ACGGC	2 cut(s)	216, 300
BciT130I	CCWGG	8 cut(s)	181, 229, 602, 628, 648, 1314, 1474, 2097
BcnI	CCSGG	2 cut(s)	57, 98
BcoDI	GTCTC	3 cut(s)	155, 2417, 2505
BfaI	CTAG	6 cut(s)	312, 1232, 1715, 2270, 2910, 2987
BfmI	CTRYAG	1 cut(s)	1344
BfoI	RGCGCY	2 cut(s)	213, 908
BfrI	CTTAAG	2 cut(s)	2107, 2885
BfuAI	ACCTGC	4 cut(s)	965, 1272, 1351, 1413
BglI	GCCNNNNNGGC	4 cut(s)	200, 546, 1768, 2047
Bme18I	GGWCC	4 cut(s)	53, 809, 1829, 2450
BmeRI	GACNNNNNGTC	1 cut(s)	1888
BmeT110I	CYCGRG	1 cut(s)	233
BmgT120I	GGNCC	5 cut(s)	53, 630, 809, 1829, 2450
BmiI	GGNNCC	7 cut(s)	55, 717, 1003, 1830, 2293, 2452, 3055
BmrI	ACTGGG	2 cut(s)	25, 502
BmsI	GCATC	9 cut(s)	385, 961, 1345, 1891, 1934, 1939, 2200, 2804, 2840
BmtI	GCTAGC	1 cut(s)	315
BmuI	ACTGGG	2 cut(s)	25, 502
BoxI	GACNNNNGTC	1 cut(s)	51
BpiI	GAAGAC	2 cut(s)	2313, 3162
BplI	GAGNNNNNCTC	2 cut(s)	1178, 1210
BpmI	CTGGAG	3 cut(s)	669, 675, 2238
Bpu10I	CCTNAGC	1 cut(s)	2339
BpuEI	CTTGAG	2 cut(s)	305, 1076
BpuMI	CCSGG	2 cut(s)	57, 98
Bsa29I	ATCGAT	1 cut(s)	2798
BsaBI	GATNNNNATC	2 cut(s)	1842, 1929
BsaHI	GRCGYC	3 cut(s)	48, 69, 1078
BsaJI	CCNNGG	7 cut(s)	180, 213, 544, 627, 633, 1473, 2033
BsaWI	WCCGGW	1 cut(s)	2820
BsaXI	ACNNNNNCTCC	2 cut(s)	2211, 2241
Bsc4I	CCNNNNNNNGG	9 cut(s)	181, 186, 219, 322, 1349, 1537, 1921, 2812, 2902
Bse1I	ACTGG	8 cut(s)	20, 497, 737, 944, 2022, 3057, 3110, 3177
Bse21I	CCTNAGG	1 cut(s)	2924
Bse3DI	GCAATG	2 cut(s)	2764, 2860
Bse8I	GATNNNNATC	2 cut(s)	1842, 1929
BseAI	TCCGGA	1 cut(s)	2820
BseBI	CCWGG	8 cut(s)	181, 229, 602, 628, 648, 1314, 1474, 2097
BseCI	ATCGAT	1 cut(s)	2798
BseDI	CCNNGG	7 cut(s)	180, 213, 544, 627, 633, 1473, 2033
BseGI	GGATG	4 cut(s)	472, 2164, 2251, 2892
BseJI	GATNNNNATC	2 cut(s)	1842, 1929
BseLI	CCNNNNNNNGG	9 cut(s)	181, 186, 219, 322, 1349, 1537, 1921, 2812, 2902
BseMI	GCAATG	2 cut(s)	2764, 2860
BseMII	CTCAG	9 cut(s)	345, 357, 849, 1149, 1235, 2330, 2403, 2882, 2938
BseNI	ACTGG	8 cut(s)	20, 497, 737, 944, 2022, 3057, 3110, 3177
BseRI	GAGGAG	6 cut(s)	152, 220, 230, 450, 776, 1144
BseYI	CCCAGC	1 cut(s)	2315
Bsh1236I	CGCG	3 cut(s)	105, 197, 558
Bsh1285I	CGRYCG	1 cut(s)	109
BshFI	GGCC	7 cut(s)	38, 60, 600, 632, 847, 943, 1987
BshNI	GGYRCC	2 cut(s)	2291, 3053
BshVI	ATCGAT	1 cut(s)	2798
BsiEI	CGRYCG	1 cut(s)	109
BsiHKAI	GWGCWC	4 cut(s)	877, 2269, 2571, 3064
BsiHKCI	CYCGRG	1 cut(s)	233
BsiSI	CCGG	3 cut(s)	57, 97, 2821
BslFI	GGGAC	4 cut(s)	39, 102, 998, 3105
BslI	CCNNNNNNNGG	9 cut(s)	181, 186, 219, 322, 1349, 1537, 1921, 2812, 2902
BsmAI	GTCTC	3 cut(s)	155, 2417, 2505
BsmFI	GGGAC	4 cut(s)	39, 102, 998, 3105
BsmI	GAATGC	1 cut(s)	450
BsnI	GGCC	7 cut(s)	38, 60, 600, 632, 847, 943, 1987
BsoBI	CYCGRG	1 cut(s)	233
Bsp1286I	GDGCHC	5 cut(s)	877, 2269, 2528, 2571, 3064
Bsp13I	TCCGGA	1 cut(s)	2820
Bsp1407I	TGTACA	1 cut(s)	3022
Bsp19I	CCATGG	1 cut(s)	544
BspACI	CCGC	8 cut(s)	5, 105, 175, 195, 250, 398, 558, 2214
BspANI	GGCC	7 cut(s)	38, 60, 600, 632, 847, 943, 1987
BspCNI	CTCAG	9 cut(s)	346, 358, 850, 1150, 1236, 2331, 2402, 2881, 2937
BspDI	ATCGAT	1 cut(s)	2798
BspEI	TCCGGA	1 cut(s)	2820
BspFNI	CGCG	3 cut(s)	105, 197, 558
BspLI	GGNNCC	7 cut(s)	55, 717, 1003, 1830, 2293, 2452, 3055
BspMAI	CTGCAG	1 cut(s)	1348
BspMI	ACCTGC	4 cut(s)	965, 1272, 1351, 1413
BspOI	GCTAGC	1 cut(s)	315
BspPI	GGATC	6 cut(s)	1217, 1475, 1548, 2089, 2191, 2783
BspQI	GCTCTTC	1 cut(s)	996
BspT107I	GGYRCC	2 cut(s)	2291, 3053
BspTI	CTTAAG	2 cut(s)	2107, 2885
BsrBI	CCGCTC	1 cut(s)	177
BsrDI	GCAATG	2 cut(s)	2764, 2860
BsrGI	TGTACA	1 cut(s)	3022
BsrI	ACTGG	8 cut(s)	20, 497, 737, 944, 2022, 3057, 3110, 3177
BssECI	CCNNGG	7 cut(s)	180, 213, 544, 627, 633, 1473, 2033
BssNAI	GTATAC	1 cut(s)	800
BssNI	GRCGYC	3 cut(s)	48, 69, 1078
BssT1I	CCWWGG	3 cut(s)	544, 633, 2033
Bst1107I	GTATAC	1 cut(s)	800
Bst2UI	CCWGG	8 cut(s)	181, 229, 602, 628, 648, 1314, 1474, 2097
Bst6I	CTCTTC	2 cut(s)	996, 2229
BstACI	GRCGYC	3 cut(s)	48, 69, 1078
BstAFI	CTTAAG	2 cut(s)	2107, 2885
BstAUI	TGTACA	1 cut(s)	3022
BstC8I	GCNNGC	6 cut(s)	248, 268, 313, 1451, 1949, 3011
BstDSI	CCRYGG	1 cut(s)	544
BstF5I	GGATG	4 cut(s)	472, 2164, 2251, 2892
BstFNI	CGCG	3 cut(s)	105, 197, 558
BstH2I	RGCGCY	2 cut(s)	213, 908
BstHHI	GCGC	2 cut(s)	212, 907
BstMAI	GTCTC	3 cut(s)	155, 2417, 2505
BstMCI	CGRYCG	1 cut(s)	109
BstMWI	GCNNNNNNNGC	8 cut(s)	200, 209, 546, 555, 913, 1278, 1768, 2047
BstNI	CCWGG	8 cut(s)	181, 229, 602, 628, 648, 1314, 1474, 2097
BstNSI	RCATGY	3 cut(s)	244, 2357, 3104
BstPAI	GACNNNNGTC	1 cut(s)	51
BstSFI	CTRYAG	1 cut(s)	1344
BstUI	CGCG	3 cut(s)	105, 197, 558
BstV2I	GAAGAC	2 cut(s)	2313, 3162
BstX2I	RGATCY	4 cut(s)	1480, 1540, 2183, 2788
BstXI	CCANNNNNNTGG	1 cut(s)	634
BstYI	RGATCY	4 cut(s)	1480, 1540, 2183, 2788
BstZ17I	GTATAC	1 cut(s)	800
Bsu15I	ATCGAT	1 cut(s)	2798
Bsu36I	CCTNAGG	1 cut(s)	2924
BsuRI	GGCC	7 cut(s)	38, 60, 600, 632, 847, 943, 1987
BsuTUI	ATCGAT	1 cut(s)	2798
BtgI	CCRYGG	1 cut(s)	544
BtsCI	GGATG	4 cut(s)	472, 2164, 2251, 2892
BtsI	GCAGTG	1 cut(s)	2563
BtsIMutI	CAGTG	6 cut(s)	744, 951, 2563, 2646, 3064, 3170
BveI	ACCTGC	4 cut(s)	965, 1272, 1351, 1413
Cac8I	GCNNGC	6 cut(s)	248, 268, 313, 1451, 1949, 3011
CfoI	GCGC	2 cut(s)	212, 907
Cfr13I	GGNCC	5 cut(s)	53, 630, 809, 1829, 2450
ClaI	ATCGAT	1 cut(s)	2798
CseI	GACGC	2 cut(s)	77, 1086
CsiI	ACCWGGT	1 cut(s)	2095
Csp6I	GTAC	3 cut(s)	2877, 3023, 3054
CviQI	GTAC	3 cut(s)	2877, 3023, 3054
DraI	TTTAAA	1 cut(s)	1629
DraIII	CACNNNGTG	1 cut(s)	2099
DriI	GACNNNNNGTC	1 cut(s)	1888
EaeI	YGGCCR	3 cut(s)	598, 941, 1985
Eam1104I	CTCTTC	2 cut(s)	996, 2229
Eam1105I	GACNNNNNGTC	1 cut(s)	1888
EarI	CTCTTC	2 cut(s)	996, 2229
EciI	GGCGGA	1 cut(s)	265
Ecl136II	GAGCTC	1 cut(s)	2569
Eco130I	CCWWGG	3 cut(s)	544, 633, 2033
Eco24I	GRGCYC	2 cut(s)	2528, 2571
Eco47I	GGWCC	4 cut(s)	53, 809, 1829, 2450
Eco47III	AGCGCT	1 cut(s)	906
Eco53kI	GAGCTC	1 cut(s)	2569
Eco57I	CTGAAG	2 cut(s)	399, 2061
Eco81I	CCTNAGG	1 cut(s)	2924
Eco88I	CYCGRG	1 cut(s)	233
EcoICRI	GAGCTC	1 cut(s)	2569
EcoO109I	RGGNCCY	3 cut(s)	630, 1829, 2450
EcoRI	GAATTC	2 cut(s)	457, 2835
EcoRII	CCWGG	8 cut(s)	179, 227, 600, 626, 646, 1312, 1472, 2095
EcoT14I	CCWWGG	3 cut(s)	544, 633, 2033
EcoT22I	ATGCAT	1 cut(s)	2776
EcoT38I	GRGCYC	2 cut(s)	2528, 2571
ErhI	CCWWGG	3 cut(s)	544, 633, 2033
FalI	AAGNNNNNCTT	6 cut(s)	750, 782, 825, 857, 2997, 3029
FaqI	GGGAC	4 cut(s)	39, 102, 998, 3105
FblI	GTMKAC	3 cut(s)	799, 867, 2625
FokI	GGATG	4 cut(s)	479, 2171, 2258, 2879
FriOI	GRGCYC	2 cut(s)	2528, 2571
FspBI	CTAG	6 cut(s)	312, 1232, 1715, 2270, 2910, 2987
GlaI	GCGC	2 cut(s)	211, 906
GsaI	CCCAGC	1 cut(s)	2319
GsuI	CTGGAG	3 cut(s)	669, 675, 2238
HaeII	RGCGCY	2 cut(s)	213, 908
HaeIII	GGCC	7 cut(s)	38, 60, 600, 632, 847, 943, 1987
HapII	CCGG	3 cut(s)	57, 97, 2821
HgaI	GACGC	2 cut(s)	77, 1086
HhaI	GCGC	2 cut(s)	212, 907
Hin1I	GRCGYC	3 cut(s)	48, 69, 1078
Hin6I	GCGC	2 cut(s)	210, 905
HinP1I	GCGC	2 cut(s)	210, 905
HincII	GTYRAC	1 cut(s)	3115
HindII	GTYRAC	1 cut(s)	3115
HindIII	AAGCTT	2 cut(s)	1052, 3068
HpaII	CCGG	3 cut(s)	57, 97, 2821
Hpy99I	CGWCG	7 cut(s)	50, 53, 68, 71, 85, 202, 321
HpyCH4IV	ACGT	4 cut(s)	48, 328, 1237, 1556
HpyF10VI	GCNNNNNNNGC	8 cut(s)	200, 209, 546, 555, 913, 1278, 1768, 2047
HpySE526I	ACGT	4 cut(s)	48, 328, 1237, 1556
Hsp92I	GRCGYC	3 cut(s)	48, 69, 1078
HspAI	GCGC	2 cut(s)	210, 905
Kpn2I	TCCGGA	1 cut(s)	2820
KpnI	GGTACC	1 cut(s)	3057
LguI	GCTCTTC	1 cut(s)	996
LmnI	GCTCC	8 cut(s)	99, 182, 207, 571, 694, 763, 872, 1001
LweI	GCATC	9 cut(s)	385, 961, 1345, 1891, 1934, 1939, 2200, 2804, 2840
MabI	ACCWGGT	1 cut(s)	2095
MaeI	CTAG	6 cut(s)	312, 1232, 1715, 2270, 2910, 2987
MaeII	ACGT	4 cut(s)	48, 328, 1237, 1556
MaeIII	GTNAC	7 cut(s)	26, 977, 1031, 1574, 2248, 2428, 2509
MbiI	CCGCTC	1 cut(s)	177
MfeI	CAATTG	1 cut(s)	1893
MflI	RGATCY	4 cut(s)	1480, 1540, 2183, 2788
MhlI	GDGCHC	5 cut(s)	877, 2269, 2528, 2571, 3064
MlsI	TGGCCA	3 cut(s)	600, 943, 1987
MluNI	TGGCCA	3 cut(s)	600, 943, 1987
MlyI	GAGTC	3 cut(s)	974, 1145, 1187
Mox20I	TGGCCA	3 cut(s)	600, 943, 1987
Mph1103I	ATGCAT	1 cut(s)	2776
MroI	TCCGGA	1 cut(s)	2820
MroXI	GAANNNNTTC	1 cut(s)	3159
MscI	TGGCCA	3 cut(s)	600, 943, 1987
MseI	TTAA	9 cut(s)	879, 1509, 1559, 1628, 1778, 2108, 2229, 2811, 2886
MslI	CAYNNNNRTG	2 cut(s)	2769, 2848
Msp20I	TGGCCA	3 cut(s)	600, 943, 1987
MspA1I	CMGCKG	1 cut(s)	2216
MspCI	CTTAAG	2 cut(s)	2107, 2885
MspI	CCGG	3 cut(s)	57, 97, 2821
MunI	CAATTG	1 cut(s)	1893
Mva1269I	GAATGC	1 cut(s)	450
MvaI	CCWGG	8 cut(s)	181, 229, 602, 628, 648, 1314, 1474, 2097
MvnI	CGCG	3 cut(s)	105, 197, 558
MwoI	GCNNNNNNNGC	8 cut(s)	200, 209, 546, 555, 913, 1278, 1768, 2047
NciI	CCSGG	2 cut(s)	57, 98
NcoI	CCATGG	1 cut(s)	544
NheI	GCTAGC	1 cut(s)	311
NlaIV	GGNNCC	7 cut(s)	55, 717, 1003, 1830, 2293, 2452, 3055
NmeAIII	GCCGAG	1 cut(s)	238
NmuCI	GTSAC	5 cut(s)	977, 1031, 1574, 2248, 2509
NsiI	ATGCAT	1 cut(s)	2776
NspI	RCATGY	3 cut(s)	244, 2357, 3104
PaeR7I	CTCGAG	1 cut(s)	233
PaqCI	CACCTGC	2 cut(s)	965, 1351
PciI	ACATGT	2 cut(s)	240, 3100
PciSI	GCTCTTC	1 cut(s)	996
PcsI	WCGNNNNNNNCGW	1 cut(s)	325
PctI	GAATGC	1 cut(s)	450
PdmI	GAANNNNTTC	1 cut(s)	3159
PfeI	GAWTC	9 cut(s)	259, 584, 733, 1254, 2072, 2087, 2102, 2460, 2747
PflFI	GACNNNGTC	1 cut(s)	2254
PfoI	TCCNGGA	2 cut(s)	96, 1312
PleI	GAGTC	3 cut(s)	974, 1145, 1187
PpsI	GAGTC	3 cut(s)	974, 1145, 1187
PpuMI	RGGWCCY	2 cut(s)	1829, 2450
PscI	ACATGT	2 cut(s)	240, 3100
PshAI	GACNNNNGTC	1 cut(s)	51
PsiI	TTATAA	1 cut(s)	1169
Psp124BI	GAGCTC	1 cut(s)	2571
Psp5II	RGGWCCY	2 cut(s)	1829, 2450
Psp6I	CCWGG	8 cut(s)	179, 227, 600, 626, 646, 1312, 1472, 2095
PspFI	CCCAGC	1 cut(s)	2315
PspGI	CCWGG	8 cut(s)	179, 227, 600, 626, 646, 1312, 1472, 2095
PspN4I	GGNNCC	7 cut(s)	55, 717, 1003, 1830, 2293, 2452, 3055
PspPI	GGNCC	5 cut(s)	53, 630, 809, 1829, 2450
PspPPI	RGGWCCY	2 cut(s)	1829, 2450
PstI	CTGCAG	1 cut(s)	1348
PsuI	RGATCY	4 cut(s)	1480, 1540, 2183, 2788
PsyI	GACNNNGTC	1 cut(s)	2254
RsaI	GTAC	3 cut(s)	2878, 3024, 3055
RsaNI	GTAC	3 cut(s)	2877, 3023, 3054
RseI	CAYNNNNRTG	2 cut(s)	2769, 2848
SacI	GAGCTC	1 cut(s)	2571
SapI	GCTCTTC	1 cut(s)	996
SaqAI	TTAA	9 cut(s)	879, 1509, 1559, 1628, 1778, 2108, 2229, 2811, 2886
Sau96I	GGNCC	5 cut(s)	53, 630, 809, 1829, 2450
SchI	GAGTC	3 cut(s)	974, 1145, 1187
SduI	GDGCHC	5 cut(s)	877, 2269, 2528, 2571, 3064
SexAI	ACCWGGT	1 cut(s)	2095
SfaNI	GCATC	9 cut(s)	385, 961, 1345, 1891, 1934, 1939, 2200, 2804, 2840
SfcI	CTRYAG	1 cut(s)	1344
Sfr274I	CTCGAG	1 cut(s)	233
SinI	GGWCC	4 cut(s)	53, 809, 1829, 2450
SlaI	CTCGAG	1 cut(s)	233
SmiMI	CAYNNNNRTG	2 cut(s)	2769, 2848
SmlI	CTYRAG	5 cut(s)	233, 284, 1055, 2107, 2885
SmoI	CTYRAG	5 cut(s)	233, 284, 1055, 2107, 2885
SsiI	CCGC	8 cut(s)	5, 105, 175, 195, 250, 398, 558, 2214
SspI	AATATT	1 cut(s)	1495
SspMI	CTAG	6 cut(s)	312, 1232, 1715, 2270, 2910, 2987
SstI	GAGCTC	1 cut(s)	2571
StyI	CCWWGG	3 cut(s)	544, 633, 2033
TaiI	ACGT	4 cut(s)	51, 331, 1240, 1559
TaqI	TCGA	8 cut(s)	45, 63, 234, 899, 2538, 2798, 2848, 3163
TaqII	GACCGA	1 cut(s)	41
TatI	WGTACW	1 cut(s)	3022
TauI	GCSGC	2 cut(s)	197, 2216
TfiI	GAWTC	9 cut(s)	259, 584, 733, 1254, 2072, 2087, 2102, 2460, 2747
Tru1I	TTAA	9 cut(s)	879, 1509, 1559, 1628, 1778, 2108, 2229, 2811, 2886
Tru9I	TTAA	9 cut(s)	879, 1509, 1559, 1628, 1778, 2108, 2229, 2811, 2886
TscAI	CASTG	6 cut(s)	744, 951, 2563, 2653, 3064, 3177
TseFI	GTSAC	5 cut(s)	977, 1031, 1574, 2248, 2509
Tsp45I	GTSAC	5 cut(s)	977, 1031, 1574, 2248, 2509
TspGWI	ACGGA	3 cut(s)	885, 1600, 2930
TspRI	CASTG	6 cut(s)	744, 951, 2563, 2653, 3064, 3177
Tth111I	GACNNNGTC	1 cut(s)	2254
Vha464I	CTTAAG	2 cut(s)	2107, 2885
VpaK11BI	GGWCC	4 cut(s)	53, 809, 1829, 2450
XapI	RAATTY	8 cut(s)	457, 518, 1322, 2066, 2327, 2403, 2826, 2835
XbaI	TCTAGA	2 cut(s)	2269, 2909
XceI	RCATGY	3 cut(s)	244, 2357, 3104
XcmI	CCANNNNNNNNNTGG	1 cut(s)	699
XhoI	CTCGAG	1 cut(s)	233
XmiI	GTMKAC	3 cut(s)	799, 867, 2625
XmnI	GAANNNNTTC	1 cut(s)	3159
XspI	CTAG	6 cut(s)	312, 1232, 1715, 2270, 2910, 2987
ZraI	GACGTC	1 cut(s)	49
Zsp2I	ATGCAT	1 cut(s)	2776

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.