Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0011804

Physical Location & Seq

Genomic Coordinates Forward (+)

57816777 .. 57824361

Gene Structure 7585 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG385200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2823 bp

ATGTCAGAAGACTTATCTGAAGGAGAGAGTAGAGACACAGTTGGTGATATTTCAGCTCATAATAGTGATAGCACTAGAGGAGGAAGAATGCGTAGAATCAATTCTATGGATGTGATGGAGAACTGGGCTGCTCATCAAAAGGAAAAGAAATTCTACATTGTGTTAATAAGCCTCCATGGCTTGATACGCGGTGAAAACATGGAGCTTGGTCGTGATTCTGATACAGGTGGCCAGGTCAAGTATGTTGTGGAACTTGCCAGGGCCTTGGGTTCAATGCCTGGAGTTTATCGCGTTGATTTACTAACAAGACAAGTTTCAGCTCCAGATGTGGATTGGAGTTATGGGGAACCAACTGAAATGTTGAATCCAGTAAACTCTGAGAATCCACAAGAGGAGCTTGGGGAGAGCACTGGGGCTTATATAGTGCGTATACCATTTGGCCCAAAAGATAAATACATTCCAAAAGAACTCCTTTGGCCACACATTCCTGAGTTTGTAGACGGAGCACTTAACCATATTATACAGTTGTCCAAAGCTCTTGGAGAGCAAATTGGAGGTGGGGAACCGGTGTGGCCAGTGGCAATTCATGGACACTATGCAGATGCAGGTGACTCTGCTGCTCTTCTGTCTGGAGCCTTGAATGTCCCAATGTTATTTACTGGTCACTCACTTGGACGAGATAAGCTTGAGCAACTTTTGAAACAAGGACGCCAATCAAGAGAAGAAATAAATACGACATACAAAATTATGAGAAGGATAGAAGCAGAGGAACTATCACTTGACGCCTCTGAGATTGTTATAACCAGCACTAGACAGGAAATAGACTCACAATGGCGCTTGTATGATGGCTTTGATCCAATACTAGAACGTAAACTGCGAGCAAGAATCAAAAGGGGCGTTAGCTGTCATGGCAGGTTTATGCCTCGCACGGTTGTAATACCTCCTGGAATGGAATTTCATCATATTATTCCACCTGCAGATGGTGATGCAGATGGGGAAGGGGAAAGAAATGATGATAGTACTGCTAATCCTGACCTGCCAATTTGGTCAGAGATTATGCGTTTCTTTACCAACCCGCGCAAGCCTATGATACTTGCTCTTGCCAGGGCAGATCCTAAGAAGAATATTACGACTTTAGTTAAAGCATTTGGAGAGTGTCGCCCTTTGAGGGATCTTTCTAACCTCACATTAATTATGGGAAACCGCGACGATATTGATGAAATGTCAAGCACAAATGCCTCCGTGCTTCTTTCTATTTTAAAGCTGATTGACAGATATGATCTGTATGGTCATGTGGCATATCCGAAACACCACAAGCAGTCTGATGTTCCTGACATCTATCGTCTAGCAGCAAAAACAAAGGGTGTTTTTATCAATCCAGCTTTCATTGAGCCATTTGGGCTTACTTTAATAGAGGCAGCAGCTCATGGCTTACCTATTGTTGCCACAAAAAATGGGGGTCCTGTTGATATACATCGGGCTCTTGATAATGGTCTGCTAGTTGACCCCCATGATCAGCAGTCAATTGCTGATGCCCTTCTGAACCTAGTTTCGGATAAGCATCTTTGGGCAAGATGCAGGCAGAATGGACTGAAAAACATTCACCTTTTCTCATGGCCAGAGCATTGTAAGACCTACTTATCTCGCATAACCAGTTGCAAACCAAGGCAGCCACAATGGCGAAGAACTGCCGCTGAAATTGATTCTTCAGGATCAGATTCACCTGATGATTCCTTAAGGGATATACAAGATATATCTTTGAACTTGAAGCTCTCTTTGGATGGTGAGAAAATTGAAGGGGCTGGATCTCTTGATAATGCTTTAGATAATGATGCCGCTGAAGGAAAAGTAAAAGAAGAGAATGCGGTTTTGACAATGTCAAAGGGTGTTCTAGAGGGCTGTCAAAAGGCTGGTGCCACAGAGAAAGAAGAGAACACTGGGGCTGGTAAATTTCCTGCACTGAGGAAAAGGAAGCATGTATTTGTAATTGCTGTGGATTGTGATACAACCTCAGAGTTCAGTGAAATTGTTGAAAAGGTTATTGAGACAGTATCAAAGGAAAAGGATGCAGGACCTATAGGATTCATACTGTCAACAGCCTATCCCATATCAGAGATACAGTCTCTTTTGGTCTCAGGGGGTTTGAGCCCATCACAGTTTGATGGTTTTATATGCAGCAGTGGTGGTGAGCTCTACTATCCATCTTCAAGCTCTGAGGATAGTCCTTCTGGGCTTCCCTTTGTGGTAGACTTGGATTATCGTTCACACACTGAATACCGTTGGGGTGGAGACGGTTTGAGAAAGACTTTGGTTCGTTGGGTCGCTTCTTTCAATGAGAAAAAAGGAGACGGACAAGTTGCAGCAGATGAATCAGGAACAACTAAGCATTGCTATGCGTTTAATGTGAAGGATCCAGCAGTGATTCCCCCTGTTAAGGAACTCCGGAAAATGATGAGAATTCAGGCTCTTCGATGCAATGTTGTATATTCTCAGAATGGTACGAGGCTGAAGGTCATCCCTGTGTTGGCTTCTAGATCACAAGCCCTCAGGTATCTGTATGTCCGTTGGGGATTGAACTTGTCAAACATTGTTGCCTTTGTGGGAGAACTAGGTGACACAGATTACGAAGGCTTGCTTGGTGGTGTACACAAGACTGTTATATTGAAGGGAGTAGGTACCAGTGCTCGTAAGCTTCATGCTAACCGGAACTTTCCTTTGGAACATGTCCTTCCAGTCGACAATCCCAATGTTGTTCAAAGTGAAGGATGCTCTGATAAAGACATTCGAGCATCACTGGTGAAACTAAGGATTCTCAAGGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0008150 GO:0008194 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010927 GO:0016020 GO:0016043 GO:0016740 GO:0016757 GO:0016758 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0035251 GO:0043062 GO:0044085 GO:0044464 GO:0045229 GO:0046524 GO:0046527 GO:0046903 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0071836 GO:0071840 GO:0071944 GO:0085029

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 S6PP Sucrose_synth

Protein Analysis

940

Amino Acids

103.86

Weight (kDa)

5.95

Isoelectric Point (pI)

44.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GT-B_Sucrose_synth	PF00862	50 - 278	3.4e-10	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	358 - 531	1.4e-23	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	362 - 519	3.3e-16	Glycosyl transferases group 1
S6PP	PF05116	682 - 873	1.6e-10	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011804)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11110
fragaria_vesca	FvH4_2g28820 FvH4_2g28820
malus_domestica	MD08G1157000.v1.1 MD15G1127900.v1.1
prunus_persica	Prupe.1G483200_v2.0.a1 Prupe.1G483200_v2.0.a1
pyrus_communis	pycom08g12930 pycom15g11500
rosa_chinensis	RchiOBHm_Chr6g0297571
rosa_laevigata	RLG00000011582
rosa_roxburghii	Rroxscaffold_7G00170350
rosa_rugosa	Rorug06G0273700 Rorug06G0273800 Rorug06G0273800
rosa_samantha	Rh6BG392800 Rh6CG398600 Rh6DG385200
rosa_wichuraiana	Rw6G033510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	800
AarI	CACCTGC	2 cut(s)	596, 982
Acc36I	ACCTGC	4 cut(s)	596, 903, 982, 1044
Acc65I	GGTACC	1 cut(s)	2675
AccB1I	GGYRCC	2 cut(s)	1913, 2675
AccI	GTMKAC	4 cut(s)	430, 498, 2247, 2736
AccII	CGCG	4 cut(s)	189, 291, 1078, 1206
AccIII	TCCGGA	1 cut(s)	2442
AciI	CCGC	6 cut(s)	189, 1076, 1204, 1692, 1836, 1865
AclWI	GGATC	7 cut(s)	848, 1106, 1179, 1720, 1813, 2405, 2418
AcoI	YGGCCR	4 cut(s)	229, 476, 572, 1616
AcsI	RAATTY	4 cut(s)	149, 953, 1949, 2457
AcuI	CTGAAG	4 cut(s)	39, 1692, 1860, 2528
AcyI	GRCGYC	2 cut(s)	709, 783
AfaI	GTAC	4 cut(s)	1021, 2500, 2646, 2677
AfiI	CCNNNNNNNGG	5 cut(s)	980, 1168, 1552, 2434, 2524
AflII	CTTAAG	1 cut(s)	1735
AflIII	ACRYGT	1 cut(s)	2722
AgeI	ACCGGT	1 cut(s)	565
AjnI	CCWGG	5 cut(s)	231, 257, 277, 943, 1103
AjuI	GAANNNNNNNTTGG	6 cut(s)	1760, 1792, 2619, 2651, 2699, 2731
Alw21I	GWGCWC	4 cut(s)	410, 508, 2193, 2686
Alw26I	GTCTC	6 cut(s)	27, 2039, 2127, 2137, 2283, 2340
AlwI	GGATC	7 cut(s)	848, 1106, 1179, 1720, 1813, 2405, 2418
Aor13HI	TCCGGA	1 cut(s)	2442
AoxI	GGCC	6 cut(s)	229, 261, 439, 476, 572, 1616
ApeKI	GCWGC	8 cut(s)	128, 617, 1349, 1418, 1421, 1669, 2175, 2360
ApoI	RAATTY	4 cut(s)	149, 953, 1949, 2457
AseI	ATTAAT	1 cut(s)	1190
AsiGI	ACCGGT	1 cut(s)	565
Asp700I	GAANNNNTTC	1 cut(s)	100
Asp718I	GGTACC	1 cut(s)	2675
AspLEI	GCGC	2 cut(s)	837, 1080
AspS9I	GGNCC	4 cut(s)	261, 440, 1460, 2072
AvaII	GGWCC	2 cut(s)	1460, 2072
AxyI	CCTNAGG	1 cut(s)	2546
BalI	TGGCCA	4 cut(s)	231, 478, 574, 1618
BamHI	GGATCC	1 cut(s)	2410
BanI	GGYRCC	2 cut(s)	1913, 2675
BanII	GRGCYC	3 cut(s)	1483, 2150, 2193
BbsI	GAAGAC	1 cut(s)	15
Bbv12I	GWGCWC	4 cut(s)	410, 508, 2193, 2686
BbvI	GCAGC	8 cut(s)	115, 604, 1361, 1430, 1433, 1681, 2187, 2372
BccI	CCATC	8 cut(s)	109, 839, 974, 986, 1775, 2156, 2158, 2209
BciT130I	CCWGG	5 cut(s)	233, 259, 279, 945, 1105
BclI	TGATCA	1 cut(s)	1513
BcoDI	GTCTC	6 cut(s)	27, 2039, 2127, 2137, 2283, 2340
BfaI	CTAG	9 cut(s)	75, 810, 863, 1346, 1499, 1547, 1892, 2532, 2609
BfmI	CTRYAG	2 cut(s)	975, 2076
BfoI	RGCGCY	1 cut(s)	838
BfrI	CTTAAG	1 cut(s)	1735
BfuAI	ACCTGC	4 cut(s)	596, 903, 982, 1044
BglI	GCCNNNNNGGC	3 cut(s)	177, 1399, 1678
BmcAI	AGTACT	1 cut(s)	1021
Bme1390I	CCNGG	5 cut(s)	233, 259, 279, 945, 1105
Bme18I	GGWCC	2 cut(s)	1460, 2072
BmgT120I	GGNCC	4 cut(s)	261, 440, 1460, 2072
BmiI	GGNNCC	7 cut(s)	348, 564, 634, 1461, 1915, 2412, 2677
BmrFI	CCNGG	5 cut(s)	233, 259, 279, 945, 1105
BmrI	ACTGGG	3 cut(s)	133, 420, 1947
BmuI	ACTGGG	3 cut(s)	133, 420, 1947
BpiI	GAAGAC	1 cut(s)	15
BpmI	CTGGAG	3 cut(s)	300, 306, 651
BpuEI	CTTGAG	2 cut(s)	707, 2798
BsaBI	GATNNNNATC	2 cut(s)	1473, 1560
BsaHI	GRCGYC	2 cut(s)	709, 783
BsaI	GGTCTC	1 cut(s)	2137
BsaJI	CCNNGG	5 cut(s)	175, 258, 264, 1104, 1664
BsaWI	WCCGGW	3 cut(s)	565, 2442, 2703
Bsc4I	CCNNNNNNNGG	5 cut(s)	980, 1168, 1552, 2434, 2524
Bse118I	RCCGGY	1 cut(s)	565
Bse21I	CCTNAGG	1 cut(s)	2546
Bse3DI	GCAATG	2 cut(s)	2386, 2482
Bse8I	GATNNNNATC	2 cut(s)	1473, 1560
BseAI	TCCGGA	1 cut(s)	2442
BseBI	CCWGG	5 cut(s)	233, 259, 279, 945, 1105
BseDI	CCNNGG	5 cut(s)	175, 258, 264, 1104, 1664
BseGI	GGATG	5 cut(s)	115, 1786, 2071, 2514, 2771
BseJI	GATNNNNATC	2 cut(s)	1473, 1560
BseLI	CCNNNNNNNGG	5 cut(s)	980, 1168, 1552, 2434, 2524
BseMI	GCAATG	2 cut(s)	2386, 2482
BseMII	CTCAG	9 cut(s)	369, 480, 780, 1952, 2025, 2148, 2205, 2504, 2560
BseRI	GAGGAG	2 cut(s)	93, 407
BseXI	GCAGC	8 cut(s)	115, 604, 1361, 1430, 1433, 1681, 2187, 2372
BsgI	GTGCAG	1 cut(s)	1941
Bsh1236I	CGCG	4 cut(s)	189, 291, 1078, 1206
BshFI	GGCC	6 cut(s)	231, 263, 441, 478, 574, 1618
BshNI	GGYRCC	2 cut(s)	1913, 2675
BshTI	ACCGGT	1 cut(s)	565
BsiHKAI	GWGCWC	4 cut(s)	410, 508, 2193, 2686
BsiSI	CCGG	3 cut(s)	566, 2443, 2704
BslFI	GGGAC	1 cut(s)	629
BslI	CCNNNNNNNGG	5 cut(s)	980, 1168, 1552, 2434, 2524
BsmAI	GTCTC	6 cut(s)	27, 2039, 2127, 2137, 2283, 2340
BsmBI	CGTCTC	2 cut(s)	2283, 2340
BsmFI	GGGAC	1 cut(s)	629
BsmI	GAATGC	2 cut(s)	93, 1867
BsnI	GGCC	6 cut(s)	231, 263, 441, 478, 574, 1618
Bso31I	GGTCTC	1 cut(s)	2137
Bsp1286I	GDGCHC	6 cut(s)	410, 508, 1483, 2150, 2193, 2686
Bsp13I	TCCGGA	1 cut(s)	2442
Bsp1407I	TGTACA	1 cut(s)	2644
Bsp143I	GATC	9 cut(s)	853, 1111, 1171, 1279, 1513, 1712, 1805, 2410, 2534
Bsp19I	CCATGG	1 cut(s)	175
BspACI	CCGC	6 cut(s)	189, 1076, 1204, 1692, 1836, 1865
BspANI	GGCC	6 cut(s)	231, 263, 441, 478, 574, 1618
BspCNI	CTCAG	9 cut(s)	370, 481, 781, 1953, 2024, 2147, 2206, 2503, 2559
BspEI	TCCGGA	1 cut(s)	2442
BspFNI	CGCG	4 cut(s)	189, 291, 1078, 1206
BspLI	GGNNCC	7 cut(s)	348, 564, 634, 1461, 1915, 2412, 2677
BspMAI	CTGCAG	1 cut(s)	979
BspMI	ACCTGC	4 cut(s)	596, 903, 982, 1044
BspPI	GGATC	7 cut(s)	848, 1106, 1179, 1720, 1813, 2405, 2418
BspQI	GCTCTTC	2 cut(s)	627, 2472
BspT107I	GGYRCC	2 cut(s)	1913, 2675
BspTI	CTTAAG	1 cut(s)	1735
BspTNI	GGTCTC	1 cut(s)	2137
BsrDI	GCAATG	2 cut(s)	2386, 2482
BsrFI	RCCGGY	1 cut(s)	565
BsrGI	TGTACA	1 cut(s)	2644
BssAI	RCCGGY	1 cut(s)	565
BssECI	CCNNGG	5 cut(s)	175, 258, 264, 1104, 1664
BssMI	GATC	9 cut(s)	853, 1111, 1171, 1279, 1513, 1712, 1805, 2410, 2534
BssNAI	GTATAC	1 cut(s)	431
BssNI	GRCGYC	2 cut(s)	709, 783
BssT1I	CCWWGG	3 cut(s)	175, 264, 1664
Bst1107I	GTATAC	1 cut(s)	431
Bst2UI	CCWGG	5 cut(s)	233, 259, 279, 945, 1105
Bst6I	CTCTTC	4 cut(s)	627, 1851, 1923, 2472
BstACI	GRCGYC	2 cut(s)	709, 783
BstAFI	CTTAAG	1 cut(s)	1735
BstAUI	TGTACA	1 cut(s)	2644
BstC8I	GCNNGC	4 cut(s)	879, 1082, 1580, 2633
BstDSI	CCRYGG	1 cut(s)	175
BstF5I	GGATG	5 cut(s)	115, 1786, 2071, 2514, 2771
BstFNI	CGCG	4 cut(s)	189, 291, 1078, 1206
BstH2I	RGCGCY	1 cut(s)	838
BstHHI	GCGC	2 cut(s)	837, 1080
BstKTI	GATC	9 cut(s)	856, 1114, 1174, 1282, 1516, 1715, 1808, 2413, 2537
BstMAI	GTCTC	6 cut(s)	27, 2039, 2127, 2137, 2283, 2340
BstMBI	GATC	9 cut(s)	853, 1111, 1171, 1279, 1513, 1712, 1805, 2410, 2534
BstMWI	GCNNNNNNNGC	5 cut(s)	177, 186, 909, 1399, 1678
BstNI	CCWGG	5 cut(s)	233, 259, 279, 945, 1105
BstNSI	RCATGY	2 cut(s)	1979, 2726
BstSCI	CCNGG	5 cut(s)	231, 257, 277, 943, 1103
BstSFI	CTRYAG	2 cut(s)	975, 2076
BstUI	CGCG	4 cut(s)	189, 291, 1078, 1206
BstV1I	GCAGC	8 cut(s)	115, 604, 1361, 1430, 1433, 1681, 2187, 2372
BstV2I	GAAGAC	1 cut(s)	15
BstX2I	RGATCY	4 cut(s)	1111, 1171, 1805, 2410
BstXI	CCANNNNNNTGG	1 cut(s)	265
BstYI	RGATCY	4 cut(s)	1111, 1171, 1805, 2410
BstZ17I	GTATAC	1 cut(s)	431
Bsu36I	CCTNAGG	1 cut(s)	2546
BsuRI	GGCC	6 cut(s)	231, 263, 441, 478, 574, 1618
BtgI	CCRYGG	1 cut(s)	175
BtsCI	GGATG	5 cut(s)	115, 1786, 2071, 2514, 2771
BtsI	GCAGTG	2 cut(s)	2185, 2424
BveI	ACCTGC	4 cut(s)	596, 903, 982, 1044
Cac8I	GCNNGC	4 cut(s)	879, 1082, 1580, 2633
CfoI	GCGC	2 cut(s)	837, 1080
Cfr10I	RCCGGY	1 cut(s)	565
Cfr13I	GGNCC	4 cut(s)	261, 440, 1460, 2072
CseI	GACGC	2 cut(s)	717, 791
Csp6I	GTAC	4 cut(s)	1020, 2499, 2645, 2676
CspAI	ACCGGT	1 cut(s)	565
CviQI	GTAC	4 cut(s)	1020, 2499, 2645, 2676
DpnI	GATC	9 cut(s)	855, 1113, 1173, 1281, 1515, 1714, 1807, 2412, 2536
DpnII	GATC	9 cut(s)	853, 1111, 1171, 1279, 1513, 1712, 1805, 2410, 2534
DraI	TTTAAA	1 cut(s)	1260
EaeI	YGGCCR	4 cut(s)	229, 476, 572, 1616
Eam1104I	CTCTTC	4 cut(s)	627, 1851, 1923, 2472
EarI	CTCTTC	4 cut(s)	627, 1851, 1923, 2472
Ecl136II	GAGCTC	1 cut(s)	2191
Eco130I	CCWWGG	3 cut(s)	175, 264, 1664
Eco24I	GRGCYC	3 cut(s)	1483, 2150, 2193
Eco31I	GGTCTC	1 cut(s)	2137
Eco47I	GGWCC	2 cut(s)	1460, 2072
Eco53kI	GAGCTC	1 cut(s)	2191
Eco57I	CTGAAG	4 cut(s)	39, 1692, 1860, 2528
Eco81I	CCTNAGG	1 cut(s)	2546
EcoICRI	GAGCTC	1 cut(s)	2191
EcoO109I	RGGNCCY	3 cut(s)	261, 1460, 2072
EcoRI	GAATTC	1 cut(s)	2457
EcoRII	CCWGG	5 cut(s)	231, 257, 277, 943, 1103
EcoT14I	CCWWGG	3 cut(s)	175, 264, 1664
EcoT38I	GRGCYC	3 cut(s)	1483, 2150, 2193
ErhI	CCWWGG	3 cut(s)	175, 264, 1664
Esp3I	CGTCTC	2 cut(s)	2283, 2340
FalI	AAGNNNNNCTT	8 cut(s)	381, 413, 456, 488, 1622, 1654, 2619, 2651
FaqI	GGGAC	1 cut(s)	629
FauI	CCCGC	1 cut(s)	1083
FbaI	TGATCA	1 cut(s)	1513
FblI	GTMKAC	4 cut(s)	430, 498, 2247, 2736
FokI	GGATG	5 cut(s)	122, 1793, 2078, 2501, 2778
FriOI	GRGCYC	3 cut(s)	1483, 2150, 2193
FspBI	CTAG	9 cut(s)	75, 810, 863, 1346, 1499, 1547, 1892, 2532, 2609
GlaI	GCGC	2 cut(s)	836, 1079
GsuI	CTGGAG	3 cut(s)	300, 306, 651
HaeII	RGCGCY	1 cut(s)	838
HaeIII	GGCC	6 cut(s)	231, 263, 441, 478, 574, 1618
HapII	CCGG	3 cut(s)	566, 2443, 2704
HgaI	GACGC	2 cut(s)	717, 791
HhaI	GCGC	2 cut(s)	837, 1080
Hin1I	GRCGYC	2 cut(s)	709, 783
Hin6I	GCGC	2 cut(s)	835, 1078
HinP1I	GCGC	2 cut(s)	835, 1078
HincII	GTYRAC	3 cut(s)	1504, 2094, 2737
HindII	GTYRAC	3 cut(s)	1504, 2094, 2737
HindIII	AAGCTT	2 cut(s)	683, 2690
HpaII	CCGG	3 cut(s)	566, 2443, 2704
Hpy99I	CGWCG	1 cut(s)	1211
HpyCH4IV	ACGT	1 cut(s)	868
HpyF10VI	GCNNNNNNNGC	5 cut(s)	177, 186, 909, 1399, 1678
HpySE526I	ACGT	1 cut(s)	868
Hsp92I	GRCGYC	2 cut(s)	709, 783
HspAI	GCGC	2 cut(s)	835, 1078
Kpn2I	TCCGGA	1 cut(s)	2442
KpnI	GGTACC	1 cut(s)	2679
Ksp22I	TGATCA	1 cut(s)	1513
Kzo9I	GATC	9 cut(s)	853, 1111, 1171, 1279, 1513, 1712, 1805, 2410, 2534
LguI	GCTCTTC	2 cut(s)	627, 2472
LmnI	GCTCC	5 cut(s)	202, 325, 394, 503, 632
Lsp1109I	GCAGC	8 cut(s)	115, 604, 1361, 1430, 1433, 1681, 2187, 2372
MaeI	CTAG	9 cut(s)	75, 810, 863, 1346, 1499, 1547, 1892, 2532, 2609
MaeII	ACGT	1 cut(s)	868
MaeIII	GTNAC	3 cut(s)	608, 662, 2612
MalI	GATC	9 cut(s)	855, 1113, 1173, 1281, 1515, 1714, 1807, 2412, 2536
MboI	GATC	9 cut(s)	853, 1111, 1171, 1279, 1513, 1712, 1805, 2410, 2534
MfeI	CAATTG	1 cut(s)	1524
MflI	RGATCY	4 cut(s)	1111, 1171, 1805, 2410
MhlI	GDGCHC	6 cut(s)	410, 508, 1483, 2150, 2193, 2686
MlsI	TGGCCA	4 cut(s)	231, 478, 574, 1618
MluNI	TGGCCA	4 cut(s)	231, 478, 574, 1618
MlyI	GAGTC	2 cut(s)	605, 818
Mox20I	TGGCCA	4 cut(s)	231, 478, 574, 1618
MroI	TCCGGA	1 cut(s)	2442
MroXI	GAANNNNTTC	1 cut(s)	100
MscI	TGGCCA	4 cut(s)	231, 478, 574, 1618
MseI	TTAA	9 cut(s)	164, 510, 1140, 1190, 1259, 1409, 1736, 2400, 2433
MslI	CAYNNNNRTG	3 cut(s)	63, 2391, 2519
Msp20I	TGGCCA	4 cut(s)	231, 478, 574, 1618
MspA1I	CMGCKG	2 cut(s)	1694, 1838
MspCI	CTTAAG	1 cut(s)	1735
MspI	CCGG	3 cut(s)	566, 2443, 2704
MspR9I	CCNGG	5 cut(s)	233, 259, 279, 945, 1105
MunI	CAATTG	1 cut(s)	1524
Mva1269I	GAATGC	2 cut(s)	93, 1867
MvaI	CCWGG	5 cut(s)	233, 259, 279, 945, 1105
MvnI	CGCG	4 cut(s)	189, 291, 1078, 1206
MwoI	GCNNNNNNNGC	5 cut(s)	177, 186, 909, 1399, 1678
NcoI	CCATGG	1 cut(s)	175
NdeII	GATC	9 cut(s)	853, 1111, 1171, 1279, 1513, 1712, 1805, 2410, 2534
NlaIV	GGNNCC	7 cut(s)	348, 564, 634, 1461, 1915, 2412, 2677
NmuCI	GTSAC	3 cut(s)	608, 662, 2612
NspI	RCATGY	2 cut(s)	1979, 2726
PaqCI	CACCTGC	2 cut(s)	596, 982
PciI	ACATGT	1 cut(s)	2722
PciSI	GCTCTTC	2 cut(s)	627, 2472
PcsI	WCGNNNNNNNCGW	1 cut(s)	874
PctI	GAATGC	2 cut(s)	93, 1867
PdmI	GAANNNNTTC	1 cut(s)	100
PflFI	GACNNNGTC	1 cut(s)	1876
PfoI	TCCNGGA	1 cut(s)	943
PinAI	ACCGGT	1 cut(s)	565
PleI	GAGTC	2 cut(s)	605, 818
PpsI	GAGTC	2 cut(s)	605, 818
PpuMI	RGGWCCY	2 cut(s)	1460, 2072
PscI	ACATGT	1 cut(s)	2722
PshBI	ATTAAT	1 cut(s)	1190
PsiI	TTATAA	1 cut(s)	800
Psp124BI	GAGCTC	1 cut(s)	2193
Psp5II	RGGWCCY	2 cut(s)	1460, 2072
Psp6I	CCWGG	5 cut(s)	231, 257, 277, 943, 1103
PspGI	CCWGG	5 cut(s)	231, 257, 277, 943, 1103
PspN4I	GGNNCC	7 cut(s)	348, 564, 634, 1461, 1915, 2412, 2677
PspPI	GGNCC	4 cut(s)	261, 440, 1460, 2072
PspPPI	RGGWCCY	2 cut(s)	1460, 2072
PstI	CTGCAG	1 cut(s)	979
PsuI	RGATCY	4 cut(s)	1111, 1171, 1805, 2410
PsyI	GACNNNGTC	1 cut(s)	1876
RsaI	GTAC	4 cut(s)	1021, 2500, 2646, 2677
RsaNI	GTAC	4 cut(s)	1020, 2499, 2645, 2676
RseI	CAYNNNNRTG	3 cut(s)	63, 2391, 2519
SacI	GAGCTC	1 cut(s)	2193
SalI	GTCGAC	1 cut(s)	2735
SapI	GCTCTTC	2 cut(s)	627, 2472
SaqAI	TTAA	9 cut(s)	164, 510, 1140, 1190, 1259, 1409, 1736, 2400, 2433
Sau3AI	GATC	9 cut(s)	853, 1111, 1171, 1279, 1513, 1712, 1805, 2410, 2534
Sau96I	GGNCC	4 cut(s)	261, 440, 1460, 2072
ScaI	AGTACT	1 cut(s)	1021
SchI	GAGTC	2 cut(s)	605, 818
ScrFI	CCNGG	5 cut(s)	233, 259, 279, 945, 1105
SduI	GDGCHC	6 cut(s)	410, 508, 1483, 2150, 2193, 2686
SfcI	CTRYAG	2 cut(s)	975, 2076
SinI	GGWCC	2 cut(s)	1460, 2072
SmiMI	CAYNNNNRTG	3 cut(s)	63, 2391, 2519
SmlI	CTYRAG	3 cut(s)	686, 1735, 2813
SmoI	CTYRAG	3 cut(s)	686, 1735, 2813
SsiI	CCGC	6 cut(s)	189, 1076, 1204, 1692, 1836, 1865
SspI	AATATT	1 cut(s)	1126
SspMI	CTAG	9 cut(s)	75, 810, 863, 1346, 1499, 1547, 1892, 2532, 2609
SstI	GAGCTC	1 cut(s)	2193
StyD4I	CCNGG	5 cut(s)	231, 257, 277, 943, 1103
StyI	CCWWGG	3 cut(s)	175, 264, 1664
TaiI	ACGT	1 cut(s)	871
TaqI	TCGA	3 cut(s)	2470, 2736, 2785
TatI	WGTACW	2 cut(s)	1019, 2644
TauI	GCSGC	2 cut(s)	1694, 1838
Tru1I	TTAA	9 cut(s)	164, 510, 1140, 1190, 1259, 1409, 1736, 2400, 2433
Tru9I	TTAA	9 cut(s)	164, 510, 1140, 1190, 1259, 1409, 1736, 2400, 2433
TseFI	GTSAC	3 cut(s)	608, 662, 2612
TseI	GCWGC	8 cut(s)	128, 617, 1349, 1418, 1421, 1669, 2175, 2360
Tsp45I	GTSAC	3 cut(s)	608, 662, 2612
TspDTI	ATGAA	7 cut(s)	575, 947, 1233, 1375, 2074, 2382, 2684
TspGWI	ACGGA	4 cut(s)	516, 1231, 2364, 2552
Tth111I	GACNNNGTC	1 cut(s)	1876
Vha464I	CTTAAG	1 cut(s)	1735
VpaK11BI	GGWCC	2 cut(s)	1460, 2072
VspI	ATTAAT	1 cut(s)	1190
XapI	RAATTY	4 cut(s)	149, 953, 1949, 2457
XbaI	TCTAGA	2 cut(s)	1891, 2531
XceI	RCATGY	2 cut(s)	1979, 2726
XcmI	CCANNNNNNNNNTGG	1 cut(s)	330
XmiI	GTMKAC	4 cut(s)	430, 498, 2247, 2736
XmnI	GAANNNNTTC	1 cut(s)	100
XspI	CTAG	9 cut(s)	75, 810, 863, 1346, 1499, 1547, 1892, 2532, 2609
ZrmI	AGTACT	1 cut(s)	1021

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.