Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0011804

Physical Location & Seq

Genomic Coordinates Forward (+)

59276825 .. 59283323

Gene Structure 6499 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ26709

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3183 bp

ATGGCGGGAAATGACTGGGTGAACAGTTACTTAGAGGCCATTCTCGACGTCGGTCCCGGCCTCGACGACGCCAAATCGTCGTCGTCGCTGCTGCTCCGGGAACGAGGTCGTTTCAGTCCCACTCGTTACTTCGTCGAAGAGGTCATCACTCGCTACGATGAGACTGATCTCCACCGCTCCTGGGTTCGAGCTGCGGCGACGGCGAGGAGCGCCGAGGAGAGGAACACCAGGCTGGAGAACATGTGCTGGCGGATTTGGAATCTTGCTCGCAAAAAGAAGCAGCTTGAGGGAGAAGAAGCTCAACGAACAGCGAGGCGTCGGGATGAGCGTGAAAGGGGAAGGAGAGAGGCAACTGAGGACATGTCAGAAGACTTATCCGAAGGAGAGAGTAGAGACACAGTTGGTGATATTTCAGCTCATAATAGTGATAGCACTAGAGGAGGAAGAATGCGTAGAATCAATTCTACGGATGTGATGGAGAACTGGGCTGCTCATCAAAAGGAAAAGAAATTCTACATTGTGTTAATAAGCCTCCATGGCTTGATACGTGGTGAAAACATGGAGCTTGGTCGTGATTCTGATACAGGTGGCCAGGTCAAGTATGTTGTGGAACTTGCCAGGGCCTTAGGTTCAATGCCTGGAGTTTATCGCGTTGATTTACTAACAAGACAAGTTTCAGCTCCAGATGTGGATTGGAGTTATGGGGAACCAACTGAAATGTTGAATCCAGTAAACTCTGAGAATCCACAAGAGGAGCTTGGGGAGAGCACTGGGGCTTATATAGTGCGTATACCATTTGGCCCAAAAGATAAATACATTCCAAAAGAACTCCTTTGGCCACACATTCCTGAGTTTGTAGATGGAGCACTTAACCATATTATACAGTTGTCCAAAGCTCTTGGAGAGCAAATTGGAGGTGGGGAACCGGTGTGGCCAGTGGCAATTCATGGACACTATGCAGATGCAGGTGACTCTGCTGCTCTTCTGTCTGGAGCCTTGAATGTCCCAATGTTATTTACTGGTCACTCACTTGGAAGAGATAAGCTTGAGCAACTTTTGAAACAAGGACGCCAATCAAGAGAAGAAATAAATACGACATACAAAATTATGAGAAGGATAGAAGCGGAGGAACTATCACTTGACGCCTCTGAGATTGTTATAACCAGCACTAGACAGGAAATAGACTCACAATGGCGCTTGTATGATGGCTTTGATCCAATACTAGAACGTAAACTGCGAGCAAGAATAAAAAGGGGCGTTAGCTGTCATGGCAGGTTTATGCCTCGCACGGTTGTAATACCTCCAGGAATGGAATTTCATCATATTATTCCACCCGCAGATGGTGATGCAGATGGGGAAGGGGAAAGAAATGATGATAGTACTGCTAATCCTGACCTGCCAATTTGGTCAGAGATTATGCGTTTCTTTACCAACCCGCGCAAGCCTATGATACTTGCTCTTGCCAGGGCAGATCCTAAGAAGAATATTACGACTTTAGTTAAAGCATTTGGAGAGTGTCGCCCTTTGAGGGATCTTTCTAACCTCACATTAATTATGGGAAACCGCGACGATATTGATGAAATGTCGAGCACAAATGCCTCTGTGCTTCTTTCTATTTTAAAGCTGATTGATAGATATGATCTGTATGGTCATGTGGCATATCCGAAACACCACAAGCAGTCTGATGTTCCTGACATCTATCGTCTAGCAGCAAAAACAAAGGGTGTTTTTATCAATCCAGCTTTCATTGAGCCATTTGGGCTTACTTTAATAGAGGCAGCAGCTCATGGCTTACCTATTGTTGCCACAAAAAATGGAGGTCCTGTTGATATACATCGGGCTCTTGATAATGGTCTGCTAGTTGACCCCCATGATCAGCAGTCAATTGCTGATGCCCTTCTGAACCTCGTTTCGGATAAGCATCTTTGGGCAAGATGCAGGCAGAATGGACTGAAAAACATTCACCTTTTCTCATGGCCAGAGCATTGTAAGACCTACTTATCTCGCATAACCAGTTGCAAACCAAGGCAGCCACAATGGCGAAGAACTGCCGCTGAAATTGATTCTTCAGGATCAGATTCACCTGGTGATTCCTTAAGAGATATACAAGATATATCTTTGAACTTGAAGCTCTCTTTGGATGGTGAGAAAATTGAAGGGGCTGGATCTCTTGATAATGCTTTAGATAATGATGCCGCTGAAGGAAAAGTAAAAGAAGAGAATGCGGTTTTGACAATGTCAAAGGGTGTTCTAGAGGGCTGTCTAAAGGCTGGTGCCACAGAGAAAGAAGAGAACACTGGGGCTGGTAAATTTCCTGCACTGAGGAAAAGGAAGCATGTATTTGTAATTGCTGTGGATTGTGATACAACCTCAGAGTTCAGTGAAATTGTTGAAAAGGTTATTGAGACAGTATCAAAGGAAAAGGATGCAGGACCTATAGGATTCATACTGTCAACAGCCTATCCCATATCAGAGATACAGTCTCTTTTGGTCTCAGGCGGTTTGAGCCCATCACAGTTTGATGGTTTTATATGCAGCAGTGGTGGTGAGCTCTATTATCCAGCTTCGAGCTCTGAGGATAGTCCTTCTGGGCTTCCCTTTGTGGTAGACTTGGATTATCGTTCACACACTGAATACCGTTGGGGTGGAGACGGTTTGAGAAAGACTTTGGTTCGTTGGGTCGCTTCTTTCAATGAGAAAAAAGGAGACGGACAAGTTGCAGCAGATGAATCAGGAACAACTAAGCATTGCTATGCGTTTATTGTGAAGGATCCAGCAGTGATTCCCCCTGTTAAGGAACTCCGGAAAATGATGAGAATTCAGGCTCTTCGATGCAATGTTGTATATTCTCAGAATGGTACGAGGCTGAAGGTCATCCCTGTGTTGGCTTCTAGATCACAAGCCCTCAGGTATCTGTATGTCCGTTGGGGATTGGACTTGTCAAACATTGTTACCTTTGTGGGAGAACTAGGTGACACAGATTACGAAGGCTTGCTTGGTGGTGTACACAAGACTGTTATATTGAAGGGAGTAGGTACCAGTGCTCGTAAGCTTCATGCTAACCGGAACTTTCCTTTGGAACATGTCCTTCCAGTCGACAATCCCAATGTTGTTCAAAGTGAAGGATCCTCTGATAAAGACATTCGAGCATCACTGGTGAAACTAAGGATTCTCAAGGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0008150 GO:0008194 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010927 GO:0016020 GO:0016043 GO:0016740 GO:0016757 GO:0016758 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0035251 GO:0043062 GO:0044085 GO:0044464 GO:0045229 GO:0046524 GO:0046527 GO:0046903 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0071836 GO:0071840 GO:0071944 GO:0085029

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 S6PP Sucrose_synth

Protein Analysis

1060

Amino Acids

117.87

Weight (kDa)

6.05

Isoelectric Point (pI)

45.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GT-B_Sucrose_synth	PF00862	170 - 397	4.2e-10	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	478 - 651	1.7e-23	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	482 - 639	4e-16	Glycosyl transferases group 1
S6PP	PF05116	803 - 993	2.6e-10	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011804)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11110
fragaria_vesca	FvH4_2g28820 FvH4_2g28820
malus_domestica	MD08G1157000.v1.1 MD15G1127900.v1.1
prunus_persica	Prupe.1G483200_v2.0.a1 Prupe.1G483200_v2.0.a1
pyrus_communis	pycom08g12930 pycom15g11500
rosa_chinensis	RchiOBHm_Chr6g0297571
rosa_laevigata	RLG00000011582
rosa_roxburghii	Rroxscaffold_7G00170350
rosa_rugosa	Rorug06G0273700 Rorug06G0273800 Rorug06G0273800
rosa_samantha	Rh6BG392800 Rh6CG398600 Rh6DG385200
rosa_wichuraiana	Rw6G033510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1160
AarI	CACCTGC	1 cut(s)	956
AatII	GACGTC	1 cut(s)	51
Acc36I	ACCTGC	3 cut(s)	956, 1263, 1404
Acc65I	GGTACC	1 cut(s)	3035
AccB1I	GGYRCC	2 cut(s)	2273, 3035
AccBSI	CCGCTC	1 cut(s)	177
AccI	GTMKAC	3 cut(s)	790, 2607, 3096
AccII	CGCG	3 cut(s)	651, 1438, 1566
AccIII	TCCGGA	1 cut(s)	2802
AclWI	GGATC	9 cut(s)	1208, 1466, 1539, 2080, 2173, 2765, 2778, 3120, 3133
AcoI	YGGCCR	4 cut(s)	589, 836, 932, 1976
AcsI	RAATTY	4 cut(s)	509, 1313, 2309, 2817
AcuI	CTGAAG	3 cut(s)	2052, 2220, 2888
AcyI	GRCGYC	5 cut(s)	48, 69, 316, 1069, 1143
AdeI	CACNNNGTG	1 cut(s)	2087
AfaI	GTAC	4 cut(s)	1381, 2860, 3006, 3037
AfiI	CCNNNNNNNGG	7 cut(s)	181, 219, 1340, 1528, 1912, 2794, 2884
AflII	CTTAAG	1 cut(s)	2095
AflIII	ACRYGT	3 cut(s)	240, 360, 3082
AgeI	ACCGGT	1 cut(s)	925
AjnI	CCWGG	8 cut(s)	179, 227, 591, 617, 637, 1303, 1463, 2083
AjuI	GAANNNNNNNTTGG	6 cut(s)	2120, 2152, 2979, 3011, 3059, 3091
Alw21I	GWGCWC	6 cut(s)	770, 868, 1592, 2553, 2573, 3046
Alw26I	GTCTC	7 cut(s)	155, 387, 2399, 2487, 2497, 2643, 2700
AlwI	GGATC	9 cut(s)	1208, 1466, 1539, 2080, 2173, 2765, 2778, 3120, 3133
Aor13HI	TCCGGA	1 cut(s)	2802
AoxI	GGCC	8 cut(s)	36, 58, 589, 621, 799, 836, 932, 1976
ApoI	RAATTY	4 cut(s)	509, 1313, 2309, 2817
AseI	ATTAAT	1 cut(s)	1550
AsiGI	ACCGGT	1 cut(s)	925
Asp700I	GAANNNNTTC	1 cut(s)	460
Asp718I	GGTACC	1 cut(s)	3035
AspLEI	GCGC	3 cut(s)	212, 1197, 1440
AspS9I	GGNCC	5 cut(s)	53, 621, 800, 1820, 2432
AsuC2I	CCSGG	2 cut(s)	57, 98
AvaII	GGWCC	3 cut(s)	53, 1820, 2432
AxyI	CCTNAGG	2 cut(s)	625, 2906
BalI	TGGCCA	4 cut(s)	591, 838, 934, 1978
BamHI	GGATCC	2 cut(s)	2770, 3125
BanI	GGYRCC	2 cut(s)	2273, 3035
BanII	GRGCYC	4 cut(s)	1843, 2510, 2553, 2573
BbsI	GAAGAC	1 cut(s)	375
Bbv12I	GWGCWC	6 cut(s)	770, 868, 1592, 2553, 2573, 3046
BccI	CCATC	8 cut(s)	469, 854, 1199, 1334, 1346, 2135, 2516, 2518
BceAI	ACGGC	1 cut(s)	216
BciT130I	CCWGG	8 cut(s)	181, 229, 593, 619, 639, 1305, 1465, 2085
BclI	TGATCA	1 cut(s)	1873
BcnI	CCSGG	2 cut(s)	57, 98
BcoDI	GTCTC	7 cut(s)	155, 387, 2399, 2487, 2497, 2643, 2700
BfaI	CTAG	8 cut(s)	435, 1170, 1223, 1706, 1859, 2252, 2892, 2969
BfmI	CTRYAG	1 cut(s)	2436
BfoI	RGCGCY	2 cut(s)	213, 1198
BfrI	CTTAAG	1 cut(s)	2095
BfuAI	ACCTGC	3 cut(s)	956, 1263, 1404
BglI	GCCNNNNNGGC	3 cut(s)	537, 1759, 2038
BmcAI	AGTACT	1 cut(s)	1381
Bme18I	GGWCC	3 cut(s)	53, 1820, 2432
BmgT120I	GGNCC	5 cut(s)	53, 621, 800, 1820, 2432
BmiI	GGNNCC	8 cut(s)	55, 708, 924, 994, 2275, 2772, 3037, 3127
BmrI	ACTGGG	4 cut(s)	25, 493, 780, 2307
BmsI	GCATC	9 cut(s)	952, 1336, 1882, 1925, 1930, 2182, 2416, 2822, 3158
BmuI	ACTGGG	4 cut(s)	25, 493, 780, 2307
BoxI	GACNNNNGTC	1 cut(s)	51
BpiI	GAAGAC	1 cut(s)	375
BpmI	CTGGAG	5 cut(s)	254, 660, 666, 1011, 1287
BpuEI	CTTGAG	3 cut(s)	305, 1067, 3158
BpuMI	CCSGG	2 cut(s)	57, 98
BsaAI	YACGTR	1 cut(s)	548
BsaBI	GATNNNNATC	2 cut(s)	1833, 1920
BsaHI	GRCGYC	5 cut(s)	48, 69, 316, 1069, 1143
BsaI	GGTCTC	1 cut(s)	2497
BsaJI	CCNNGG	6 cut(s)	180, 213, 535, 618, 1464, 2024
BsaWI	WCCGGW	3 cut(s)	925, 2802, 3063
BsaXI	ACNNNNNCTCC	4 cut(s)	227, 257, 1809, 1839
Bsc4I	CCNNNNNNNGG	7 cut(s)	181, 219, 1340, 1528, 1912, 2794, 2884
Bse118I	RCCGGY	1 cut(s)	925
Bse21I	CCTNAGG	2 cut(s)	625, 2906
Bse3DI	GCAATG	2 cut(s)	2746, 2842
Bse8I	GATNNNNATC	2 cut(s)	1833, 1920
BseAI	TCCGGA	1 cut(s)	2802
BseBI	CCWGG	8 cut(s)	181, 229, 593, 619, 639, 1305, 1465, 2085
BseDI	CCNNGG	6 cut(s)	180, 213, 535, 618, 1464, 2024
BseGI	GGATG	5 cut(s)	328, 475, 2146, 2431, 2874
BseJI	GATNNNNATC	2 cut(s)	1833, 1920
BseLI	CCNNNNNNNGG	7 cut(s)	181, 219, 1340, 1528, 1912, 2794, 2884
BseMI	GCAATG	2 cut(s)	2746, 2842
BseRI	GAGGAG	4 cut(s)	220, 230, 453, 767
BsgI	GTGCAG	1 cut(s)	2301
Bsh1236I	CGCG	3 cut(s)	651, 1438, 1566
BshFI	GGCC	8 cut(s)	38, 60, 591, 623, 801, 838, 934, 1978
BshNI	GGYRCC	2 cut(s)	2273, 3035
BshTI	ACCGGT	1 cut(s)	925
BsiHKAI	GWGCWC	6 cut(s)	770, 868, 1592, 2553, 2573, 3046
BsiSI	CCGG	5 cut(s)	57, 97, 926, 2803, 3064
BslFI	GGGAC	3 cut(s)	39, 102, 989
BslI	CCNNNNNNNGG	7 cut(s)	181, 219, 1340, 1528, 1912, 2794, 2884
BsmAI	GTCTC	7 cut(s)	155, 387, 2399, 2487, 2497, 2643, 2700
BsmBI	CGTCTC	2 cut(s)	2643, 2700
BsmFI	GGGAC	3 cut(s)	39, 102, 989
BsmI	GAATGC	2 cut(s)	453, 2227
BsnI	GGCC	8 cut(s)	38, 60, 591, 623, 801, 838, 934, 1978
Bso31I	GGTCTC	1 cut(s)	2497
Bsp1286I	GDGCHC	8 cut(s)	770, 868, 1592, 1843, 2510, 2553, 2573, 3046
Bsp13I	TCCGGA	1 cut(s)	2802
Bsp1407I	TGTACA	1 cut(s)	3004
Bsp19I	CCATGG	1 cut(s)	535
BspANI	GGCC	8 cut(s)	38, 60, 591, 623, 801, 838, 934, 1978
BspEI	TCCGGA	1 cut(s)	2802
BspFNI	CGCG	3 cut(s)	651, 1438, 1566
BspLI	GGNNCC	8 cut(s)	55, 708, 924, 994, 2275, 2772, 3037, 3127
BspMI	ACCTGC	3 cut(s)	956, 1263, 1404
BspPI	GGATC	9 cut(s)	1208, 1466, 1539, 2080, 2173, 2765, 2778, 3120, 3133
BspQI	GCTCTTC	2 cut(s)	987, 2832
BspT107I	GGYRCC	2 cut(s)	2273, 3035
BspTI	CTTAAG	1 cut(s)	2095
BspTNI	GGTCTC	1 cut(s)	2497
BsrBI	CCGCTC	1 cut(s)	177
BsrDI	GCAATG	2 cut(s)	2746, 2842
BsrFI	RCCGGY	1 cut(s)	925
BsrGI	TGTACA	1 cut(s)	3004
BssAI	RCCGGY	1 cut(s)	925
BssECI	CCNNGG	6 cut(s)	180, 213, 535, 618, 1464, 2024
BssNAI	GTATAC	1 cut(s)	791
BssNI	GRCGYC	5 cut(s)	48, 69, 316, 1069, 1143
BssT1I	CCWWGG	2 cut(s)	535, 2024
Bst1107I	GTATAC	1 cut(s)	791
Bst2UI	CCWGG	8 cut(s)	181, 229, 593, 619, 639, 1305, 1465, 2085
Bst6I	CTCTTC	6 cut(s)	132, 987, 1030, 2211, 2283, 2832
BstACI	GRCGYC	5 cut(s)	48, 69, 316, 1069, 1143
BstAFI	CTTAAG	1 cut(s)	2095
BstAUI	TGTACA	1 cut(s)	3004
BstBAI	YACGTR	1 cut(s)	548
BstC8I	GCNNGC	6 cut(s)	248, 268, 1239, 1442, 1940, 2993
BstDSI	CCRYGG	1 cut(s)	535
BstF5I	GGATG	5 cut(s)	328, 475, 2146, 2431, 2874
BstFNI	CGCG	3 cut(s)	651, 1438, 1566
BstH2I	RGCGCY	2 cut(s)	213, 1198
BstHHI	GCGC	3 cut(s)	212, 1197, 1440
BstMAI	GTCTC	7 cut(s)	155, 387, 2399, 2487, 2497, 2643, 2700
BstMWI	GCNNNNNNNGC	7 cut(s)	200, 209, 537, 1269, 1759, 2038, 2505
BstNI	CCWGG	8 cut(s)	181, 229, 593, 619, 639, 1305, 1465, 2085
BstNSI	RCATGY	4 cut(s)	244, 364, 2339, 3086
BstPAI	GACNNNNGTC	1 cut(s)	51
BstSFI	CTRYAG	1 cut(s)	2436
BstUI	CGCG	3 cut(s)	651, 1438, 1566
BstV2I	GAAGAC	1 cut(s)	375
BstX2I	RGATCY	5 cut(s)	1471, 1531, 2165, 2770, 3125
BstYI	RGATCY	5 cut(s)	1471, 1531, 2165, 2770, 3125
BstZ17I	GTATAC	1 cut(s)	791
Bsu36I	CCTNAGG	2 cut(s)	625, 2906
BsuRI	GGCC	8 cut(s)	38, 60, 591, 623, 801, 838, 934, 1978
BtgI	CCRYGG	1 cut(s)	535
BtsCI	GGATG	5 cut(s)	328, 475, 2146, 2431, 2874
BtsI	GCAGTG	2 cut(s)	2545, 2784
BveI	ACCTGC	3 cut(s)	956, 1263, 1404
Cac8I	GCNNGC	6 cut(s)	248, 268, 1239, 1442, 1940, 2993
CfoI	GCGC	3 cut(s)	212, 1197, 1440
Cfr10I	RCCGGY	1 cut(s)	925
Cfr13I	GGNCC	5 cut(s)	53, 621, 800, 1820, 2432
CseI	GACGC	4 cut(s)	77, 305, 1077, 1151
CsiI	ACCWGGT	1 cut(s)	2083
Csp6I	GTAC	4 cut(s)	1380, 2859, 3005, 3036
CspAI	ACCGGT	1 cut(s)	925
CviQI	GTAC	4 cut(s)	1380, 2859, 3005, 3036
DraI	TTTAAA	1 cut(s)	1620
DraIII	CACNNNGTG	1 cut(s)	2087
EaeI	YGGCCR	4 cut(s)	589, 836, 932, 1976
Eam1104I	CTCTTC	6 cut(s)	132, 987, 1030, 2211, 2283, 2832
EarI	CTCTTC	6 cut(s)	132, 987, 1030, 2211, 2283, 2832
EciI	GGCGGA	1 cut(s)	265
Ecl136II	GAGCTC	2 cut(s)	2551, 2571
Eco130I	CCWWGG	2 cut(s)	535, 2024
Eco24I	GRGCYC	4 cut(s)	1843, 2510, 2553, 2573
Eco31I	GGTCTC	1 cut(s)	2497
Eco47I	GGWCC	3 cut(s)	53, 1820, 2432
Eco53kI	GAGCTC	2 cut(s)	2551, 2571
Eco57I	CTGAAG	3 cut(s)	2052, 2220, 2888
Eco81I	CCTNAGG	2 cut(s)	625, 2906
EcoICRI	GAGCTC	2 cut(s)	2551, 2571
EcoO109I	RGGNCCY	3 cut(s)	621, 1820, 2432
EcoRI	GAATTC	1 cut(s)	2817
EcoRII	CCWGG	8 cut(s)	179, 227, 591, 617, 637, 1303, 1463, 2083
EcoT14I	CCWWGG	2 cut(s)	535, 2024
EcoT38I	GRGCYC	4 cut(s)	1843, 2510, 2553, 2573
ErhI	CCWWGG	2 cut(s)	535, 2024
Esp3I	CGTCTC	2 cut(s)	2643, 2700
FalI	AAGNNNNNCTT	8 cut(s)	741, 773, 816, 848, 1982, 2014, 2979, 3011
FaqI	GGGAC	3 cut(s)	39, 102, 989
FauI	CCCGC	2 cut(s)	1342, 1443
FbaI	TGATCA	1 cut(s)	1873
FblI	GTMKAC	3 cut(s)	790, 2607, 3096
FokI	GGATG	5 cut(s)	335, 482, 2153, 2438, 2861
FriOI	GRGCYC	4 cut(s)	1843, 2510, 2553, 2573
FspBI	CTAG	8 cut(s)	435, 1170, 1223, 1706, 1859, 2252, 2892, 2969
GlaI	GCGC	3 cut(s)	211, 1196, 1439
GsuI	CTGGAG	5 cut(s)	254, 660, 666, 1011, 1287
HaeII	RGCGCY	2 cut(s)	213, 1198
HaeIII	GGCC	8 cut(s)	38, 60, 591, 623, 801, 838, 934, 1978
HapII	CCGG	5 cut(s)	57, 97, 926, 2803, 3064
HgaI	GACGC	4 cut(s)	77, 305, 1077, 1151
HhaI	GCGC	3 cut(s)	212, 1197, 1440
Hin1I	GRCGYC	5 cut(s)	48, 69, 316, 1069, 1143
Hin6I	GCGC	3 cut(s)	210, 1195, 1438
HinP1I	GCGC	3 cut(s)	210, 1195, 1438
HincII	GTYRAC	3 cut(s)	1864, 2454, 3097
HindII	GTYRAC	3 cut(s)	1864, 2454, 3097
HindIII	AAGCTT	2 cut(s)	1043, 3050
HpaII	CCGG	5 cut(s)	57, 97, 926, 2803, 3064
HpyCH4IV	ACGT	3 cut(s)	48, 547, 1228
HpyF10VI	GCNNNNNNNGC	7 cut(s)	200, 209, 537, 1269, 1759, 2038, 2505
HpySE526I	ACGT	3 cut(s)	48, 547, 1228
Hsp92I	GRCGYC	5 cut(s)	48, 69, 316, 1069, 1143
HspAI	GCGC	3 cut(s)	210, 1195, 1438
Kpn2I	TCCGGA	1 cut(s)	2802
KpnI	GGTACC	1 cut(s)	3039
Ksp22I	TGATCA	1 cut(s)	1873
LguI	GCTCTTC	2 cut(s)	987, 2832
LmnI	GCTCC	8 cut(s)	99, 182, 207, 562, 685, 754, 863, 992
LweI	GCATC	9 cut(s)	952, 1336, 1882, 1925, 1930, 2182, 2416, 2822, 3158
MabI	ACCWGGT	1 cut(s)	2083
MaeI	CTAG	8 cut(s)	435, 1170, 1223, 1706, 1859, 2252, 2892, 2969
MaeII	ACGT	3 cut(s)	48, 547, 1228
MaeIII	GTNAC	6 cut(s)	26, 125, 968, 1022, 2950, 2972
MbiI	CCGCTC	1 cut(s)	177
MfeI	CAATTG	1 cut(s)	1884
MflI	RGATCY	5 cut(s)	1471, 1531, 2165, 2770, 3125
MhlI	GDGCHC	8 cut(s)	770, 868, 1592, 1843, 2510, 2553, 2573, 3046
MlsI	TGGCCA	4 cut(s)	591, 838, 934, 1978
MluNI	TGGCCA	4 cut(s)	591, 838, 934, 1978
MlyI	GAGTC	2 cut(s)	965, 1178
Mox20I	TGGCCA	4 cut(s)	591, 838, 934, 1978
MroI	TCCGGA	1 cut(s)	2802
MroXI	GAANNNNTTC	1 cut(s)	460
MscI	TGGCCA	4 cut(s)	591, 838, 934, 1978
MseI	TTAA	8 cut(s)	524, 870, 1500, 1550, 1619, 1769, 2096, 2793
MslI	CAYNNNNRTG	3 cut(s)	423, 2751, 2879
Msp20I	TGGCCA	4 cut(s)	591, 838, 934, 1978
MspA1I	CMGCKG	2 cut(s)	2054, 2198
MspCI	CTTAAG	1 cut(s)	2095
MspI	CCGG	5 cut(s)	57, 97, 926, 2803, 3064
MunI	CAATTG	1 cut(s)	1884
Mva1269I	GAATGC	2 cut(s)	453, 2227
MvaI	CCWGG	8 cut(s)	181, 229, 593, 619, 639, 1305, 1465, 2085
MvnI	CGCG	3 cut(s)	651, 1438, 1566
MwoI	GCNNNNNNNGC	7 cut(s)	200, 209, 537, 1269, 1759, 2038, 2505
NciI	CCSGG	2 cut(s)	57, 98
NcoI	CCATGG	1 cut(s)	535
NlaIV	GGNNCC	8 cut(s)	55, 708, 924, 994, 2275, 2772, 3037, 3127
NmeAIII	GCCGAG	1 cut(s)	238
NmuCI	GTSAC	3 cut(s)	968, 1022, 2972
NspI	RCATGY	4 cut(s)	244, 364, 2339, 3086
PaqCI	CACCTGC	1 cut(s)	956
PciI	ACATGT	3 cut(s)	240, 360, 3082
PciSI	GCTCTTC	2 cut(s)	987, 2832
PcsI	WCGNNNNNNNCGW	2 cut(s)	325, 1234
PctI	GAATGC	2 cut(s)	453, 2227
PdmI	GAANNNNTTC	1 cut(s)	460
PflFI	GACNNNGTC	1 cut(s)	2236
PfoI	TCCNGGA	2 cut(s)	96, 1303
PinAI	ACCGGT	1 cut(s)	925
PleI	GAGTC	2 cut(s)	965, 1178
PpsI	GAGTC	2 cut(s)	965, 1178
Ppu21I	YACGTR	1 cut(s)	548
PpuMI	RGGWCCY	2 cut(s)	1820, 2432
PscI	ACATGT	3 cut(s)	240, 360, 3082
PshAI	GACNNNNGTC	1 cut(s)	51
PshBI	ATTAAT	1 cut(s)	1550
PsiI	TTATAA	1 cut(s)	1160
Psp124BI	GAGCTC	2 cut(s)	2553, 2573
Psp5II	RGGWCCY	2 cut(s)	1820, 2432
Psp6I	CCWGG	8 cut(s)	179, 227, 591, 617, 637, 1303, 1463, 2083
PspGI	CCWGG	8 cut(s)	179, 227, 591, 617, 637, 1303, 1463, 2083
PspN4I	GGNNCC	8 cut(s)	55, 708, 924, 994, 2275, 2772, 3037, 3127
PspPI	GGNCC	5 cut(s)	53, 621, 800, 1820, 2432
PspPPI	RGGWCCY	2 cut(s)	1820, 2432
PsuI	RGATCY	5 cut(s)	1471, 1531, 2165, 2770, 3125
PsyI	GACNNNGTC	1 cut(s)	2236
RsaI	GTAC	4 cut(s)	1381, 2860, 3006, 3037
RsaNI	GTAC	4 cut(s)	1380, 2859, 3005, 3036
RseI	CAYNNNNRTG	3 cut(s)	423, 2751, 2879
SacI	GAGCTC	2 cut(s)	2553, 2573
SalI	GTCGAC	1 cut(s)	3095
SapI	GCTCTTC	2 cut(s)	987, 2832
SaqAI	TTAA	8 cut(s)	524, 870, 1500, 1550, 1619, 1769, 2096, 2793
Sau96I	GGNCC	5 cut(s)	53, 621, 800, 1820, 2432
ScaI	AGTACT	1 cut(s)	1381
SchI	GAGTC	2 cut(s)	965, 1178
SduI	GDGCHC	8 cut(s)	770, 868, 1592, 1843, 2510, 2553, 2573, 3046
SexAI	ACCWGGT	1 cut(s)	2083
SfaNI	GCATC	9 cut(s)	952, 1336, 1882, 1925, 1930, 2182, 2416, 2822, 3158
SfcI	CTRYAG	1 cut(s)	2436
SinI	GGWCC	3 cut(s)	53, 1820, 2432
SmiMI	CAYNNNNRTG	3 cut(s)	423, 2751, 2879
SmlI	CTYRAG	4 cut(s)	284, 1046, 2095, 3173
SmoI	CTYRAG	4 cut(s)	284, 1046, 2095, 3173
SspI	AATATT	1 cut(s)	1486
SspMI	CTAG	8 cut(s)	435, 1170, 1223, 1706, 1859, 2252, 2892, 2969
SstI	GAGCTC	2 cut(s)	2553, 2573
StyI	CCWWGG	2 cut(s)	535, 2024
TaiI	ACGT	3 cut(s)	51, 550, 1231
TaqI	TCGA	9 cut(s)	45, 63, 135, 187, 1586, 2567, 2830, 3096, 3145
TaqII	GACCGA	1 cut(s)	41
TatI	WGTACW	2 cut(s)	1379, 3004
TauI	GCSGC	3 cut(s)	197, 2054, 2198
Tru1I	TTAA	8 cut(s)	524, 870, 1500, 1550, 1619, 1769, 2096, 2793
Tru9I	TTAA	8 cut(s)	524, 870, 1500, 1550, 1619, 1769, 2096, 2793
TseFI	GTSAC	3 cut(s)	968, 1022, 2972
Tsp45I	GTSAC	3 cut(s)	968, 1022, 2972
TspDTI	ATGAA	7 cut(s)	935, 1307, 1593, 1735, 2434, 2742, 3044
TspGWI	ACGGA	3 cut(s)	482, 2724, 2912
Tth111I	GACNNNGTC	1 cut(s)	2236
Vha464I	CTTAAG	1 cut(s)	2095
VpaK11BI	GGWCC	3 cut(s)	53, 1820, 2432
VspI	ATTAAT	1 cut(s)	1550
XapI	RAATTY	4 cut(s)	509, 1313, 2309, 2817
XbaI	TCTAGA	2 cut(s)	2251, 2891
XceI	RCATGY	4 cut(s)	244, 364, 2339, 3086
XcmI	CCANNNNNNNNNTGG	1 cut(s)	690
XmiI	GTMKAC	3 cut(s)	790, 2607, 3096
XmnI	GAANNNNTTC	1 cut(s)	460
XspI	CTAG	8 cut(s)	435, 1170, 1223, 1706, 1859, 2252, 2892, 2969
ZraI	GACGTC	1 cut(s)	49
ZrmI	AGTACT	1 cut(s)	1381

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.