Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0011804

Physical Location & Seq

Genomic Coordinates Forward (+)

58061351 .. 58067834

Gene Structure 6484 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G033510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3186 bp

ATGGCGGGAAATGACTGGGTGAACAGTTACTTAGAGGCCATTCTCGACGTCGGTCCCGGCCTCGACGACGCCAAATCTTCGTCGTCGCTGCTGCTCCGGGAACGAGGTCGTTTCAGTCCCACTCGTTACTTCGTCGAAGAGGTCATCACTCGCTACGATGAGACTGATCTCCACCGCTCCTGGGTTCGAGCTGCGGCGACGGCGAGGAGCGCCGAGGAGAGGAACACCAGGCTGGAGAACATGTGCTGGCGGATTTGGAATCTTGCTCGCAAAAAGAAGCAGCTTGAGGGAGAAGAAGCTCAACGAACAGCGAGGCGTCGGGATGAGCGTGAAAGGGGAAGGAGAGAGGCAACTGAGGACATGTCAGAAGACTTATCTGAAGGAGAGAGTAGAGACACAGTTGGTGATATTTCAGCTCATAATAGTGATCATAGCACTAGAGGAGGAAGAATGCGTAGAATCAATTCTATGGATGTGATGGAGAACTGGGCTGCTCATCAAAAGGAAAAGAAATTCTACATTGTGTTAATAAGCCTCCATGGCTTGATTCGCGGTGAAAACATGGAGCTTGGTCGTGATTCTGATACAGGTGGCCAGGTCAAGTATGTTGTGGAACTTGCCAGGGCCTTGGGTTCAATGCCTGGAGTTTATCGCGTTGATTTACTAACAAGACAAGTTTCATCTCCAGATGTGGATTGGAGTTATGGGGAACCAACTGAAATGTTGAATCCAGTAAACTCTGAGAATCCACAAGAGGAGCTTGGGGAGAGTACTGGGGCTTATATAGTACGTATACCATTTGGCCCAAAAGATAAATACATTCCCAAAGAACTCCTTTGGCCACACATTCCTGAGTTTGTAGACGGAGCACTTAACCATATTATACAGTTGTCCAAAGCTCTTGGCGAGCAAATTGGAGGTGGGGAACCGGTGTGGCCGGTGGCAATTCATGGACACTATGCAGATGCAGGTGACTCTGCTGCTCTTCTGTCTGGAGCCTTGAATGTCCCAATGTTATTTACTGGTCACTCACTTGGAAGAGATAAGCTTGAGCAACTTTTGAAACAAGGACGCCAATCAAGAGAAGAAATAAATACGACATACAAAATTATGAGAAGGATAGAAGCAGAGGAACTATCACTTGACGCCTCTGAGATTGTTATAACCAGCACTAGGCAGGAAATAGACTCACAATGGCGCTTGTATGATGGCTTTGATCCAATACTAGAACGTAAACTGCGAGCAAGAATAAAAAGGGGCGTTAGCTGTCATGGCAGGTTTATGCCTCGCACGGTTGTAATACCTCCTGGAATGGAATTTCATCATATTATTCCACCCGCAGATGGTGATGCAGATGGGGAAGGGGAAAGAAATGATGATAGTACTGCTAATCCTGACCTGCCAATTTGGTCAGAGATTATGCGTTTCTTTACCAACCCGCGCAAGCCTATGATACTTGCTCTTGCCAGGGCAGATCCTAAGAAGAATATTACGACTTTAGTTAAAGCATTTGGAGAGTGTCGCCCTTTGAGGGATCTTTCTAACCTCACATTAATTATGGGAAACCGCGACGATATTGATGAAATGTCGAGCACAAATGCCTCTGTGCTTCTTTCTATTTTAAAGCTGATTGACAGATATGATCTGTATGGTCATGTGGCATATCCGAAACACCACAAGCAGTCTGATGTTCCTGACATCTATCGTCTAGCAGCAAAAACAAAGGGTGTTTTTATCAATCCAGCTTTCATTGAGCCATTTGGGCTTACTTTAATAGAGGCAGCAGCTCATGGCTTACCTATTGTTGCCACAAAAAATGGGGGTCCTGTTGATATACATCGGGCTCTTGATAATGGTCTGCTAGTTGACCCCCATGATCAGCAGTCAATTGCTGATGCCCTTCTGAACCTCGTTTCGGATAAGCATCTTTGGGCAAGATGCAGGCAGAATGGACTGAAAAACATTCACCTTTTCTCATGGCCAGAGCATTGTAAGACCTACTTATCTCGCATAACCAGTTGCAAACCAAGGCAGCCACAGTGGCGAAGAACTGCCGCCGAAATTGATTCTTCAGGATCAGATTCACCTGGTGATTCCTTAAGAGATATACAAGATATATCTTTGAACTTGAAGCTCTCTTTGGATGGTGAGAAAATTGAAGGGGCTGGATCTCTTGATAATGCTTTAGATAATGATGCTGCTGAAGGAAAAGTAAAAGAAGAGAATGCGGTTTTGACAATGTCAAAGGGTGTTCTAGAGGGCTGTCAAAAGGCTGGTGCCACAGAGAAAGAAGAGAACACTGGGGCTGGTAAATTTCCTGCACTGAGGAAAAGGAAGCATGTATTTGTAATTGCTGTGGATTGTGATACAACCTCAGAGTTCAGTGAAATTGTTGAAAAGGTTATTGAGACAGTATCAAAGGAAAAGGATGCAGGACCTATAGGATTCATACTGTCAACAGCCTATCCCATAGCAGAGATACAGTCTCTTTTGGTCTCAGGGGGTTTGAGCCCATCACAGTTTGATGGTTTTATATGCAGCAGTGGTGGTGAGCTCTATTATCCAGCTTCAAGCTCTGAGGATAGTCCTTCTGGGCTTCCCTTTGTGGTAGACTTGGATTATCGTTCACACACTGAATATCGTTGGGGTGGAGACGGTTTGAGAAAGACTTTGGTTCGTTGGGTCGCTTCTTTCAATGAGAAAAAAGGAGACGGACAAGTTGCAGCAGATGAATCAGGAACAACTAAGCATTGCTATGCGTTTATTGTGAAGGATCCAGCAGTGATTCCCCCTGTTAAGGAACTCCGGAAAATGATGAGAATTCAGGCTCTTCGATGCAATGTTGTATATTCTCAGAATGGTACGAGGCTGAAGGTCATCCCTGTGTTGGCTTCTAGATCACAAGCCCTCAGGTATCTGTATGTCCGTTGGGGATTGGACTTGTCAAACATTGTTACCTTTGTGGGAGAACTAGGTGACACAGATTACGAAGGCTTGCTTGGTGGTGTACACAAGACTGTTATATTGAAGGGAGTAGGTACCAGTGCTCGTAAGCTTCATGCTAACCGGAACTTTCCATTGGAACATGTCCTTCCAGTCGACAATCCCAATGTTGTTCAAAGTGAAGGATGCTCTGATAAAGACATTCGAGCATCACTGGTGAAACTAAGGATTCTCAAGGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0008150 GO:0008194 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010927 GO:0016020 GO:0016043 GO:0016740 GO:0016757 GO:0016758 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0035251 GO:0043062 GO:0044085 GO:0044464 GO:0045229 GO:0046524 GO:0046527 GO:0046903 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0071836 GO:0071840 GO:0071944 GO:0085029

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 S6PP Sucrose_synth

Protein Analysis

1061

Amino Acids

118.06

Weight (kDa)

6.07

Isoelectric Point (pI)

45.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GT-B_Sucrose_synth	PF00862	171 - 398	4e-10	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	479 - 652	1.7e-23	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	483 - 640	4e-16	Glycosyl transferases group 1
S6PP	PF05116	804 - 994	2.6e-10	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011804)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11110
fragaria_vesca	FvH4_2g28820 FvH4_2g28820
malus_domestica	MD08G1157000.v1.1 MD15G1127900.v1.1
prunus_persica	Prupe.1G483200_v2.0.a1 Prupe.1G483200_v2.0.a1
pyrus_communis	pycom08g12930 pycom15g11500
rosa_chinensis	RchiOBHm_Chr6g0297571
rosa_laevigata	RLG00000011582
rosa_roxburghii	Rroxscaffold_7G00170350
rosa_rugosa	Rorug06G0273700 Rorug06G0273800 Rorug06G0273800
rosa_samantha	Rh6BG392800 Rh6CG398600 Rh6DG385200
rosa_wichuraiana	Rw6G033510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1163
AarI	CACCTGC	1 cut(s)	959
AatII	GACGTC	1 cut(s)	51
Acc36I	ACCTGC	3 cut(s)	959, 1266, 1407
Acc65I	GGTACC	1 cut(s)	3038
AccB1I	GGYRCC	2 cut(s)	2276, 3038
AccBSI	CCGCTC	1 cut(s)	177
AccI	GTMKAC	4 cut(s)	793, 861, 2610, 3099
AccII	CGCG	4 cut(s)	552, 654, 1441, 1569
AccIII	TCCGGA	1 cut(s)	2805
AclWI	GGATC	7 cut(s)	1211, 1469, 1542, 2083, 2176, 2768, 2781
AcoI	YGGCCR	4 cut(s)	592, 839, 935, 1979
AcsI	RAATTY	4 cut(s)	512, 1316, 2312, 2820
AcuI	CTGAAG	4 cut(s)	399, 2055, 2223, 2891
AcyI	GRCGYC	5 cut(s)	48, 69, 316, 1072, 1146
AdeI	CACNNNGTG	1 cut(s)	2090
AfaI	GTAC	6 cut(s)	772, 789, 1384, 2863, 3009, 3040
AfiI	CCNNNNNNNGG	8 cut(s)	181, 219, 1173, 1343, 1531, 1915, 2797, 2887
AflII	CTTAAG	1 cut(s)	2098
AflIII	ACRYGT	3 cut(s)	240, 360, 3085
AgeI	ACCGGT	1 cut(s)	928
AjnI	CCWGG	8 cut(s)	179, 227, 594, 620, 640, 1306, 1466, 2086
AjuI	GAANNNNNNNTTGG	6 cut(s)	2123, 2155, 2982, 3014, 3062, 3094
Alw21I	GWGCWC	4 cut(s)	871, 1595, 2556, 3049
Alw26I	GTCTC	7 cut(s)	155, 387, 2402, 2490, 2500, 2646, 2703
AlwI	GGATC	7 cut(s)	1211, 1469, 1542, 2083, 2176, 2768, 2781
Aor13HI	TCCGGA	1 cut(s)	2805
AoxI	GGCC	8 cut(s)	36, 58, 592, 624, 802, 839, 935, 1979
ApoI	RAATTY	4 cut(s)	512, 1316, 2312, 2820
AseI	ATTAAT	1 cut(s)	1553
AsiGI	ACCGGT	1 cut(s)	928
Asp700I	GAANNNNTTC	1 cut(s)	463
Asp718I	GGTACC	1 cut(s)	3038
AspLEI	GCGC	3 cut(s)	212, 1200, 1443
AspS9I	GGNCC	5 cut(s)	53, 624, 803, 1823, 2435
AsuC2I	CCSGG	2 cut(s)	57, 98
AvaII	GGWCC	3 cut(s)	53, 1823, 2435
AxyI	CCTNAGG	1 cut(s)	2909
BalI	TGGCCA	3 cut(s)	594, 841, 1981
BamHI	GGATCC	1 cut(s)	2773
BanI	GGYRCC	2 cut(s)	2276, 3038
BanII	GRGCYC	3 cut(s)	1846, 2513, 2556
BbsI	GAAGAC	1 cut(s)	375
Bbv12I	GWGCWC	4 cut(s)	871, 1595, 2556, 3049
BccI	CCATC	7 cut(s)	472, 1202, 1337, 1349, 2138, 2519, 2521
BceAI	ACGGC	1 cut(s)	216
BciT130I	CCWGG	8 cut(s)	181, 229, 596, 622, 642, 1308, 1468, 2088
BclI	TGATCA	2 cut(s)	427, 1876
BcnI	CCSGG	2 cut(s)	57, 98
BcoDI	GTCTC	7 cut(s)	155, 387, 2402, 2490, 2500, 2646, 2703
BfaI	CTAG	8 cut(s)	438, 1173, 1226, 1709, 1862, 2255, 2895, 2972
BfmI	CTRYAG	1 cut(s)	2439
BfoI	RGCGCY	2 cut(s)	213, 1201
BfrI	CTTAAG	1 cut(s)	2098
BfuAI	ACCTGC	3 cut(s)	959, 1266, 1407
BglI	GCCNNNNNGGC	3 cut(s)	540, 1762, 2041
BmcAI	AGTACT	2 cut(s)	772, 1384
Bme18I	GGWCC	3 cut(s)	53, 1823, 2435
BmgT120I	GGNCC	5 cut(s)	53, 624, 803, 1823, 2435
BmiI	GGNNCC	8 cut(s)	55, 711, 927, 997, 1824, 2278, 2775, 3040
BmrI	ACTGGG	4 cut(s)	25, 496, 783, 2310
BmuI	ACTGGG	4 cut(s)	25, 496, 783, 2310
BoxI	GACNNNNGTC	1 cut(s)	51
BpiI	GAAGAC	1 cut(s)	375
BpmI	CTGGAG	4 cut(s)	254, 663, 669, 1014
BpuEI	CTTGAG	3 cut(s)	305, 1070, 3161
BpuMI	CCSGG	2 cut(s)	57, 98
BsaAI	YACGTR	1 cut(s)	791
BsaBI	GATNNNNATC	2 cut(s)	1836, 1923
BsaHI	GRCGYC	5 cut(s)	48, 69, 316, 1072, 1146
BsaI	GGTCTC	1 cut(s)	2500
BsaJI	CCNNGG	7 cut(s)	180, 213, 538, 621, 627, 1467, 2027
BsaWI	WCCGGW	3 cut(s)	928, 2805, 3066
BsaXI	ACNNNNNCTCC	2 cut(s)	227, 257
Bsc4I	CCNNNNNNNGG	8 cut(s)	181, 219, 1173, 1343, 1531, 1915, 2797, 2887
Bse118I	RCCGGY	2 cut(s)	928, 937
Bse21I	CCTNAGG	1 cut(s)	2909
Bse3DI	GCAATG	2 cut(s)	2749, 2845
Bse8I	GATNNNNATC	2 cut(s)	1836, 1923
BseAI	TCCGGA	1 cut(s)	2805
BseBI	CCWGG	8 cut(s)	181, 229, 596, 622, 642, 1308, 1468, 2088
BseDI	CCNNGG	7 cut(s)	180, 213, 538, 621, 627, 1467, 2027
BseGI	GGATG	6 cut(s)	328, 478, 2149, 2434, 2877, 3134
BseJI	GATNNNNATC	2 cut(s)	1836, 1923
BseLI	CCNNNNNNNGG	8 cut(s)	181, 219, 1173, 1343, 1531, 1915, 2797, 2887
BseMI	GCAATG	2 cut(s)	2749, 2845
BseRI	GAGGAG	4 cut(s)	220, 230, 456, 770
BsgI	GTGCAG	1 cut(s)	2304
Bsh1236I	CGCG	4 cut(s)	552, 654, 1441, 1569
BshFI	GGCC	8 cut(s)	38, 60, 594, 626, 804, 841, 937, 1981
BshNI	GGYRCC	2 cut(s)	2276, 3038
BshTI	ACCGGT	1 cut(s)	928
BsiHKAI	GWGCWC	4 cut(s)	871, 1595, 2556, 3049
BsiSI	CCGG	6 cut(s)	57, 97, 929, 938, 2806, 3067
BslFI	GGGAC	3 cut(s)	39, 102, 992
BslI	CCNNNNNNNGG	8 cut(s)	181, 219, 1173, 1343, 1531, 1915, 2797, 2887
BsmAI	GTCTC	7 cut(s)	155, 387, 2402, 2490, 2500, 2646, 2703
BsmBI	CGTCTC	2 cut(s)	2646, 2703
BsmFI	GGGAC	3 cut(s)	39, 102, 992
BsmI	GAATGC	2 cut(s)	456, 2230
BsnI	GGCC	8 cut(s)	38, 60, 594, 626, 804, 841, 937, 1981
Bso31I	GGTCTC	1 cut(s)	2500
Bsp1286I	GDGCHC	6 cut(s)	871, 1595, 1846, 2513, 2556, 3049
Bsp13I	TCCGGA	1 cut(s)	2805
Bsp1407I	TGTACA	1 cut(s)	3007
Bsp19I	CCATGG	1 cut(s)	538
BspANI	GGCC	8 cut(s)	38, 60, 594, 626, 804, 841, 937, 1981
BspEI	TCCGGA	1 cut(s)	2805
BspFNI	CGCG	4 cut(s)	552, 654, 1441, 1569
BspLI	GGNNCC	8 cut(s)	55, 711, 927, 997, 1824, 2278, 2775, 3040
BspMI	ACCTGC	3 cut(s)	959, 1266, 1407
BspPI	GGATC	7 cut(s)	1211, 1469, 1542, 2083, 2176, 2768, 2781
BspQI	GCTCTTC	2 cut(s)	990, 2835
BspT107I	GGYRCC	2 cut(s)	2276, 3038
BspTI	CTTAAG	1 cut(s)	2098
BspTNI	GGTCTC	1 cut(s)	2500
BsrBI	CCGCTC	1 cut(s)	177
BsrDI	GCAATG	2 cut(s)	2749, 2845
BsrFI	RCCGGY	2 cut(s)	928, 937
BsrGI	TGTACA	1 cut(s)	3007
BssAI	RCCGGY	2 cut(s)	928, 937
BssECI	CCNNGG	7 cut(s)	180, 213, 538, 621, 627, 1467, 2027
BssNAI	GTATAC	1 cut(s)	794
BssNI	GRCGYC	5 cut(s)	48, 69, 316, 1072, 1146
BssT1I	CCWWGG	3 cut(s)	538, 627, 2027
Bst1107I	GTATAC	1 cut(s)	794
Bst2UI	CCWGG	8 cut(s)	181, 229, 596, 622, 642, 1308, 1468, 2088
Bst6I	CTCTTC	6 cut(s)	132, 990, 1033, 2214, 2286, 2835
BstACI	GRCGYC	5 cut(s)	48, 69, 316, 1072, 1146
BstAFI	CTTAAG	1 cut(s)	2098
BstAUI	TGTACA	1 cut(s)	3007
BstBAI	YACGTR	1 cut(s)	791
BstC8I	GCNNGC	7 cut(s)	248, 268, 908, 1242, 1445, 1943, 2996
BstDSI	CCRYGG	1 cut(s)	538
BstF5I	GGATG	6 cut(s)	328, 478, 2149, 2434, 2877, 3134
BstFNI	CGCG	4 cut(s)	552, 654, 1441, 1569
BstH2I	RGCGCY	2 cut(s)	213, 1201
BstHHI	GCGC	3 cut(s)	212, 1200, 1443
BstMAI	GTCTC	7 cut(s)	155, 387, 2402, 2490, 2500, 2646, 2703
BstMWI	GCNNNNNNNGC	7 cut(s)	200, 209, 540, 549, 1272, 1762, 2041
BstNI	CCWGG	8 cut(s)	181, 229, 596, 622, 642, 1308, 1468, 2088
BstNSI	RCATGY	4 cut(s)	244, 364, 2342, 3089
BstPAI	GACNNNNGTC	1 cut(s)	51
BstSFI	CTRYAG	1 cut(s)	2439
BstSNI	TACGTA	1 cut(s)	791
BstUI	CGCG	4 cut(s)	552, 654, 1441, 1569
BstV2I	GAAGAC	1 cut(s)	375
BstX2I	RGATCY	4 cut(s)	1474, 1534, 2168, 2773
BstXI	CCANNNNNNTGG	1 cut(s)	628
BstYI	RGATCY	4 cut(s)	1474, 1534, 2168, 2773
BstZ17I	GTATAC	1 cut(s)	794
Bsu36I	CCTNAGG	1 cut(s)	2909
BsuRI	GGCC	8 cut(s)	38, 60, 594, 626, 804, 841, 937, 1981
BtgI	CCRYGG	1 cut(s)	538
BtsCI	GGATG	6 cut(s)	328, 478, 2149, 2434, 2877, 3134
BtsI	GCAGTG	2 cut(s)	2548, 2787
BtsIMutI	CAGTG	9 cut(s)	2045, 2298, 2321, 2389, 2548, 2631, 2787, 3049, 3155
BveI	ACCTGC	3 cut(s)	959, 1266, 1407
Cac8I	GCNNGC	7 cut(s)	248, 268, 908, 1242, 1445, 1943, 2996
CfoI	GCGC	3 cut(s)	212, 1200, 1443
Cfr10I	RCCGGY	2 cut(s)	928, 937
Cfr13I	GGNCC	5 cut(s)	53, 624, 803, 1823, 2435
CseI	GACGC	4 cut(s)	77, 305, 1080, 1154
CsiI	ACCWGGT	1 cut(s)	2086
Csp6I	GTAC	6 cut(s)	771, 788, 1383, 2862, 3008, 3039
CspAI	ACCGGT	1 cut(s)	928
CviQI	GTAC	6 cut(s)	771, 788, 1383, 2862, 3008, 3039
DraI	TTTAAA	1 cut(s)	1623
DraIII	CACNNNGTG	1 cut(s)	2090
EaeI	YGGCCR	4 cut(s)	592, 839, 935, 1979
Eam1104I	CTCTTC	6 cut(s)	132, 990, 1033, 2214, 2286, 2835
EarI	CTCTTC	6 cut(s)	132, 990, 1033, 2214, 2286, 2835
EciI	GGCGGA	1 cut(s)	265
Ecl136II	GAGCTC	1 cut(s)	2554
Eco105I	TACGTA	1 cut(s)	791
Eco130I	CCWWGG	3 cut(s)	538, 627, 2027
Eco24I	GRGCYC	3 cut(s)	1846, 2513, 2556
Eco31I	GGTCTC	1 cut(s)	2500
Eco47I	GGWCC	3 cut(s)	53, 1823, 2435
Eco53kI	GAGCTC	1 cut(s)	2554
Eco57I	CTGAAG	4 cut(s)	399, 2055, 2223, 2891
Eco81I	CCTNAGG	1 cut(s)	2909
EcoICRI	GAGCTC	1 cut(s)	2554
EcoO109I	RGGNCCY	3 cut(s)	624, 1823, 2435
EcoRI	GAATTC	1 cut(s)	2820
EcoRII	CCWGG	8 cut(s)	179, 227, 594, 620, 640, 1306, 1466, 2086
EcoT14I	CCWWGG	3 cut(s)	538, 627, 2027
EcoT38I	GRGCYC	3 cut(s)	1846, 2513, 2556
ErhI	CCWWGG	3 cut(s)	538, 627, 2027
Esp3I	CGTCTC	2 cut(s)	2646, 2703
FalI	AAGNNNNNCTT	8 cut(s)	744, 776, 819, 851, 1985, 2017, 2982, 3014
FaqI	GGGAC	3 cut(s)	39, 102, 992
FauI	CCCGC	2 cut(s)	1345, 1446
FbaI	TGATCA	2 cut(s)	427, 1876
FblI	GTMKAC	4 cut(s)	793, 861, 2610, 3099
FokI	GGATG	6 cut(s)	335, 485, 2156, 2441, 2864, 3141
FriOI	GRGCYC	3 cut(s)	1846, 2513, 2556
FspBI	CTAG	8 cut(s)	438, 1173, 1226, 1709, 1862, 2255, 2895, 2972
GlaI	GCGC	3 cut(s)	211, 1199, 1442
GsuI	CTGGAG	4 cut(s)	254, 663, 669, 1014
HaeII	RGCGCY	2 cut(s)	213, 1201
HaeIII	GGCC	8 cut(s)	38, 60, 594, 626, 804, 841, 937, 1981
HapII	CCGG	6 cut(s)	57, 97, 929, 938, 2806, 3067
HgaI	GACGC	4 cut(s)	77, 305, 1080, 1154
HhaI	GCGC	3 cut(s)	212, 1200, 1443
Hin1I	GRCGYC	5 cut(s)	48, 69, 316, 1072, 1146
Hin6I	GCGC	3 cut(s)	210, 1198, 1441
HinP1I	GCGC	3 cut(s)	210, 1198, 1441
HincII	GTYRAC	3 cut(s)	1867, 2457, 3100
HindII	GTYRAC	3 cut(s)	1867, 2457, 3100
HindIII	AAGCTT	2 cut(s)	1046, 3053
HpaII	CCGG	6 cut(s)	57, 97, 929, 938, 2806, 3067
HpyCH4IV	ACGT	3 cut(s)	48, 790, 1231
HpyF10VI	GCNNNNNNNGC	7 cut(s)	200, 209, 540, 549, 1272, 1762, 2041
HpySE526I	ACGT	3 cut(s)	48, 790, 1231
Hsp92I	GRCGYC	5 cut(s)	48, 69, 316, 1072, 1146
HspAI	GCGC	3 cut(s)	210, 1198, 1441
Kpn2I	TCCGGA	1 cut(s)	2805
KpnI	GGTACC	1 cut(s)	3042
Ksp22I	TGATCA	2 cut(s)	427, 1876
LguI	GCTCTTC	2 cut(s)	990, 2835
LmnI	GCTCC	7 cut(s)	99, 182, 207, 565, 757, 866, 995
MabI	ACCWGGT	1 cut(s)	2086
MaeI	CTAG	8 cut(s)	438, 1173, 1226, 1709, 1862, 2255, 2895, 2972
MaeII	ACGT	3 cut(s)	48, 790, 1231
MaeIII	GTNAC	6 cut(s)	26, 125, 971, 1025, 2953, 2975
MbiI	CCGCTC	1 cut(s)	177
MfeI	CAATTG	1 cut(s)	1887
MflI	RGATCY	4 cut(s)	1474, 1534, 2168, 2773
MhlI	GDGCHC	6 cut(s)	871, 1595, 1846, 2513, 2556, 3049
MlsI	TGGCCA	3 cut(s)	594, 841, 1981
MluNI	TGGCCA	3 cut(s)	594, 841, 1981
MlyI	GAGTC	2 cut(s)	968, 1181
Mox20I	TGGCCA	3 cut(s)	594, 841, 1981
MroI	TCCGGA	1 cut(s)	2805
MroXI	GAANNNNTTC	1 cut(s)	463
MscI	TGGCCA	3 cut(s)	594, 841, 1981
MseI	TTAA	8 cut(s)	527, 873, 1503, 1553, 1622, 1772, 2099, 2796
MslI	CAYNNNNRTG	3 cut(s)	423, 2754, 2882
Msp20I	TGGCCA	3 cut(s)	594, 841, 1981
MspCI	CTTAAG	1 cut(s)	2098
MspI	CCGG	6 cut(s)	57, 97, 929, 938, 2806, 3067
MunI	CAATTG	1 cut(s)	1887
Mva1269I	GAATGC	2 cut(s)	456, 2230
MvaI	CCWGG	8 cut(s)	181, 229, 596, 622, 642, 1308, 1468, 2088
MvnI	CGCG	4 cut(s)	552, 654, 1441, 1569
MwoI	GCNNNNNNNGC	7 cut(s)	200, 209, 540, 549, 1272, 1762, 2041
NciI	CCSGG	2 cut(s)	57, 98
NcoI	CCATGG	1 cut(s)	538
NlaIV	GGNNCC	8 cut(s)	55, 711, 927, 997, 1824, 2278, 2775, 3040
NmeAIII	GCCGAG	1 cut(s)	238
NmuCI	GTSAC	3 cut(s)	971, 1025, 2975
NspI	RCATGY	4 cut(s)	244, 364, 2342, 3089
PaqCI	CACCTGC	1 cut(s)	959
PciI	ACATGT	3 cut(s)	240, 360, 3085
PciSI	GCTCTTC	2 cut(s)	990, 2835
PcsI	WCGNNNNNNNCGW	2 cut(s)	325, 1237
PctI	GAATGC	2 cut(s)	456, 2230
PdmI	GAANNNNTTC	1 cut(s)	463
PflFI	GACNNNGTC	1 cut(s)	2239
PfoI	TCCNGGA	2 cut(s)	96, 1306
PinAI	ACCGGT	1 cut(s)	928
PleI	GAGTC	2 cut(s)	968, 1181
PpsI	GAGTC	2 cut(s)	968, 1181
Ppu21I	YACGTR	1 cut(s)	791
PpuMI	RGGWCCY	2 cut(s)	1823, 2435
PscI	ACATGT	3 cut(s)	240, 360, 3085
PshAI	GACNNNNGTC	1 cut(s)	51
PshBI	ATTAAT	1 cut(s)	1553
PsiI	TTATAA	1 cut(s)	1163
Psp124BI	GAGCTC	1 cut(s)	2556
Psp5II	RGGWCCY	2 cut(s)	1823, 2435
Psp6I	CCWGG	8 cut(s)	179, 227, 594, 620, 640, 1306, 1466, 2086
PspGI	CCWGG	8 cut(s)	179, 227, 594, 620, 640, 1306, 1466, 2086
PspN4I	GGNNCC	8 cut(s)	55, 711, 927, 997, 1824, 2278, 2775, 3040
PspPI	GGNCC	5 cut(s)	53, 624, 803, 1823, 2435
PspPPI	RGGWCCY	2 cut(s)	1823, 2435
PsuI	RGATCY	4 cut(s)	1474, 1534, 2168, 2773
PsyI	GACNNNGTC	1 cut(s)	2239
RsaI	GTAC	6 cut(s)	772, 789, 1384, 2863, 3009, 3040
RsaNI	GTAC	6 cut(s)	771, 788, 1383, 2862, 3008, 3039
RseI	CAYNNNNRTG	3 cut(s)	423, 2754, 2882
SacI	GAGCTC	1 cut(s)	2556
SalI	GTCGAC	1 cut(s)	3098
SapI	GCTCTTC	2 cut(s)	990, 2835
SaqAI	TTAA	8 cut(s)	527, 873, 1503, 1553, 1622, 1772, 2099, 2796
Sau96I	GGNCC	5 cut(s)	53, 624, 803, 1823, 2435
ScaI	AGTACT	2 cut(s)	772, 1384
SchI	GAGTC	2 cut(s)	968, 1181
SduI	GDGCHC	6 cut(s)	871, 1595, 1846, 2513, 2556, 3049
SexAI	ACCWGGT	1 cut(s)	2086
SfcI	CTRYAG	1 cut(s)	2439
SinI	GGWCC	3 cut(s)	53, 1823, 2435
SmiMI	CAYNNNNRTG	3 cut(s)	423, 2754, 2882
SmlI	CTYRAG	4 cut(s)	284, 1049, 2098, 3176
SmoI	CTYRAG	4 cut(s)	284, 1049, 2098, 3176
SnaBI	TACGTA	1 cut(s)	791
SspI	AATATT	1 cut(s)	1489
SspMI	CTAG	8 cut(s)	438, 1173, 1226, 1709, 1862, 2255, 2895, 2972
SstI	GAGCTC	1 cut(s)	2556
StyI	CCWWGG	3 cut(s)	538, 627, 2027
TaiI	ACGT	3 cut(s)	51, 793, 1234
TaqI	TCGA	8 cut(s)	45, 63, 135, 187, 1589, 2833, 3099, 3148
TaqII	GACCGA	1 cut(s)	41
TatI	WGTACW	3 cut(s)	770, 1382, 3007
TauI	GCSGC	2 cut(s)	197, 2057
Tru1I	TTAA	8 cut(s)	527, 873, 1503, 1553, 1622, 1772, 2099, 2796
Tru9I	TTAA	8 cut(s)	527, 873, 1503, 1553, 1622, 1772, 2099, 2796
TscAI	CASTG	9 cut(s)	2045, 2305, 2328, 2389, 2548, 2638, 2787, 3049, 3162
TseFI	GTSAC	3 cut(s)	971, 1025, 2975
Tsp45I	GTSAC	3 cut(s)	971, 1025, 2975
TspDTI	ATGAA	8 cut(s)	669, 938, 1310, 1596, 1738, 2437, 2745, 3047
TspGWI	ACGGA	3 cut(s)	879, 2727, 2915
TspRI	CASTG	9 cut(s)	2045, 2305, 2328, 2389, 2548, 2638, 2787, 3049, 3162
Tth111I	GACNNNGTC	1 cut(s)	2239
Vha464I	CTTAAG	1 cut(s)	2098
VpaK11BI	GGWCC	3 cut(s)	53, 1823, 2435
VspI	ATTAAT	1 cut(s)	1553
XapI	RAATTY	4 cut(s)	512, 1316, 2312, 2820
XbaI	TCTAGA	2 cut(s)	2254, 2894
XceI	RCATGY	4 cut(s)	244, 364, 2342, 3089
XcmI	CCANNNNNNNNNTGG	1 cut(s)	693
XmiI	GTMKAC	4 cut(s)	793, 861, 2610, 3099
XmnI	GAANNNNTTC	1 cut(s)	463
XspI	CTAG	8 cut(s)	438, 1173, 1226, 1709, 1862, 2255, 2895, 2972
ZraI	GACGTC	1 cut(s)	49
ZrmI	AGTACT	2 cut(s)	772, 1384

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.