Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011804

Physical Location & Seq

Genomic Coordinates Reverse (-)

9254140 .. 9260078

Gene Structure 5939 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1127900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3174 bp

ATGGCGGGAAACGACTGGGTGAACAGTTACTTGGAGGCGATCCTTGACGTCGGTCCGGGCCTTGACGACGCCAAATCGTCGCTTCTCCTCCGTGAACGCGGCCGTTTCAGTCCGACTCGTTACTTCGTCGAAGAGGTGATCACTCGCTACGATGAGACCGATCTCCACCGCTCCTGGGTTCGGGCTGCGGCGACGGCGAGGAGTCCGGAGGAGAGGAATACCAGGTTGGAGAATATGTGCTGGCGGATTTGGAATTTGGCTCGCAAAAAGAAGCAGATTGAGGGAGAAGAAGCTCAGCGTATGGCCAGACATCGTCTTGAAAGGGAACGAGGCAGGAGAGAAGCGACTGAGGATATGTCAGAAGACTTGTCTGAGGGAGAGAAAGGAGACACGGTCGGTGACATTTCAGCTCATGGTGGTGATAGCACTAGAGGAGGAAGAATGAAAAGAATCAATTCTACTGATGCAGTGGAGAACTGGACTAGTCAACAAAAAGAACAGAAATTCTACATGGTGTTAATAAGCCTTCATGGCCTGATCCGTGGCGAGAACATGGAGCTTGGTCGTGATTCTGATACTGGTGGCCAGGTCAAGTATGTAGTAGAACTTGCCAGGGCCTTAGGTTCAATGCCTGGAGTTTATCGGGTTGATTTGCTAACAAGACAAGTGGCAGCTCCAGATGTGGATTGGAGTTATGGGGAACCAACTGAAATGCTGAATCCATTAAACACTGAGAATTCAAAGGAGGAGCTTGGGGAGAGTAGTGGAGCTTATATAGTCCGGATACCATTTGGCCCAAGGGATAAATATGTTCCAAAAGAACTCCTTTGGCCTCACATACCTGAGTTTGTTGATGGTGCACTTACCCACATTCTACAGATGTCTAAAGCTCTGGGAGAGCAAATTGGAGGTGGGCAACCAGTTTGGCCCGTTGCAATTCATGGACATTATGCAGATGCAGGTGATTCTGCTGCTCTTTTATCTGGTGCCTTGAATGTCCCAATGGTATTTACTGGTCACTCACTTGGACGAGATAAGCTTGAACAACTTTTGAAACAAGGACGTCAATCAAGAGAAGAAATAAATACAACGTACAAAATAATGAGACGGATAGAAGCAGAGGAGTTAACCCTAGATGCCTCTGAGATTGTTATCACAAGCACTAGACAGGAGATAGAATCACAATGGCGCTTGTATGATGGCTTTGATCCAATACTAGAACGCAAACTAAGAGCAAGAATCAAAAGGGGTGTAAGCTGTTACGGCAGGTTTATGCCTCGCATGGTTGTAATACCTCCAGGAATGGAGTTTCATCATATCATTCCACATGATGGCGACGGGGATGGTGAAGGGGAAAGACATGATGATAGTTCTACTTCTCCTGACCCTCCAATTTGGTCGGAGATCATGCGTTTCTTTACCAATCCGCGGAAGCCTATGATACTTGCCCTTGCCAGGGCAGACCCTAAAAAGAATATTACGACCTTAGTCAAAGCATTTGGAGAGTGTCGCCCACTGAGGGAACTTGCTAACCTTACATTGATAATGGGAAACCGTGATGACATTGATGAAATGTCCAGCACAAATGCATCTGTGCTTCTTTCCATTCTTAAGCTGATTGACAGATACGATCTTTACGGTCATGTGGCATATCCGAAACACCACAAGCAGTCTGATGTTCCTGACATTTACCGTCTGGCAGCAAAGACAAAGGGTGTTTTCATCAATCCAGCTTTCATCGAGCCATTTGGACTTACTTTAATTGAGGCAGCAGCTCATGGCTTACCTATTGTTGCCACACAAAATGGGGGTCCTGTTGATATTCATCGGGTTCTTGACAATGGTCTACTAGTTGACCCCCATGATCAGCAGTCAATAGCGGATGCCCTTCTGAAGCTTGTTTCGGACAAGCAACTTTGGGCAAGATGCAGGCAGAATGGACTGAAAAACATTCACCTTTTCTCATGGCCAGAGCATTGTAAGACATACTTAACTCGCATAACCAGTTGCAAACCAAGGCAGCCACAGTGGCAAAGAAATGAAGCTGACTTTGACAATTCACAACCCGATTCACCTAGTGATTCTTTGAGGGATATACAAGATATATCTTTGAACTTGAAACTCTCACTGGATGGCGACAAAACTGAAGGGAGTGCTGCTCTTGATAATGCTTTAGAGACTGAAGACCATGCTGCTGGAGGGAAGATTAAGGAGCAAAATGCGGTTTTGACATTGTCAAAAGGTGTTTGTGAAAAGGCTGGTTCCACAGAGAAAGCAGACAACAGTTCTGGGGCTGGTAAATTTCCAGCATTCAGGAAAAGGAAGTATGTTTATGTAATTGCTGTGGATTGTGATACAACCTCAGAGTTTACTGAAATCATTGAGAAGGTTACTGAGGCAACAGAGAAGGACAAAGATGCAGGACCTATCGGATTTATACTATCAACAGCATTGGGCATATCTGAGATACATACTCTTTTGGTCTCAGGAGGTTTGAGTCCTTCACAGTTTGATGCTTTTATCTGTAACAGCGGTGGTGAGCTCTACTATCCATCTTCAAGTTCTGAGGATAGTCCTTCTGGGCTTCCCTTTGTTGTGGACTTAGATTACCGTTCACACATTGAATACCGCTGGGGAGCAGAAGGTTTGAGAAAGACGTTGGTTCGTTGGGTCGCTAATTTCAACGAAAAGAAAGGAAGCGAAACTGTTACAGAAGATGTATCAGCGTCAACTAACCATTGCTATGCGTATAAAGTGAAAGATCCAGCACTGATTCCCCCTGTTAAGGAACTCCGGAGACTCATGAGAATTCAGGCTCTCCGTTGCCACGTTATATATTCCCAAAATGGCACCAGGCTAAATGTTATCCCTGTGTTGGCTTCTAGATCACAAGCTCTCAGGTACCTGTATGTCCGTTGGGGCTTGAACTTGTCAACTGCTGTTGTCTTCGTGGGAGAAAGTGGTGACACGGATTATGAAGGCTTGCTTGGTGGGCTACACAAAACTGTGATACTGAAGGGAGTTAGCAGTGGCGCTCGTAAGCTTCATGCCAACAGAAACTATCCTTTAGAACATATCTTTCCAGACGACAGTCCCAATATGGCTCAAAGTGAAGGATGCTCCCAAAATGACATAAGAGCATCATTGGTGAAACTAGGGATTCTTAAGCGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0008150 GO:0008194 GO:0009555 GO:0009653 GO:0009987 GO:0010208 GO:0010927 GO:0016020 GO:0016043 GO:0016740 GO:0016757 GO:0016758 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0035251 GO:0043062 GO:0044085 GO:0044464 GO:0045229 GO:0046524 GO:0046527 GO:0046903 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0071836 GO:0071840 GO:0071944 GO:0085029

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glycos_transf_1 S6PP Sucrose_synth

Protein Analysis

1058

Amino Acids

118.01

Weight (kDa)

6.04

Isoelectric Point (pI)

49.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GT-B_Sucrose_synth	PF00862	168 - 437	4.3e-10	Sucrose synthase first GT-B domain
Glycos_transf_1	PF00534	475 - 648	1.9e-23	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	479 - 636	5.7e-16	Glycosyl transferases group 1
S6PP	PF05116	791 - 993	1.4e-11	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011804)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11110
fragaria_vesca	FvH4_2g28820 FvH4_2g28820
malus_domestica	MD08G1157000.v1.1 MD15G1127900.v1.1
prunus_persica	Prupe.1G483200_v2.0.a1 Prupe.1G483200_v2.0.a1
pyrus_communis	pycom08g12930 pycom15g11500
rosa_chinensis	RchiOBHm_Chr6g0297571
rosa_laevigata	RLG00000011582
rosa_roxburghii	Rroxscaffold_7G00170350
rosa_rugosa	Rorug06G0273700 Rorug06G0273800 Rorug06G0273800
rosa_samantha	Rh6BG392800 Rh6CG398600 Rh6DG385200
rosa_wichuraiana	Rw6G033510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	950
AatII	GACGTC	2 cut(s)	51, 1066
Acc36I	ACCTGC	2 cut(s)	950, 1257
Acc65I	GGTACC	1 cut(s)	2901
AccB1I	GGYRCC	3 cut(s)	986, 2849, 2901
AccB7I	CCANNNNNTGG	1 cut(s)	1331
AccBSI	CCGCTC	1 cut(s)	171
AccI	GTMKAC	1 cut(s)	1846
AccII	CGCG	2 cut(s)	99, 1429
AccIII	TCCGGA	3 cut(s)	205, 780, 2793
AclWI	GGATC	4 cut(s)	34, 532, 1202, 2756
AcoI	YGGCCR	4 cut(s)	100, 303, 583, 1967
AcsI	RAATTY	5 cut(s)	253, 503, 736, 2300, 2808
AcuI	CTGAAG	4 cut(s)	1913, 2166, 2202, 3035
AcyI	GRCGYC	3 cut(s)	48, 69, 1063
AdeI	CACNNNGTG	1 cut(s)	2078
AfaI	GTAC	2 cut(s)	1094, 2903
AfiI	CCNNNNNNNGG	9 cut(s)	175, 180, 1331, 1428, 1455, 1456, 1519, 2785, 2875
AflII	CTTAAG	2 cut(s)	1610, 3164
AhlI	ACTAGT	2 cut(s)	482, 1849
AjnI	CCWGG	8 cut(s)	173, 221, 585, 611, 631, 1297, 1454, 2852
AjuI	GAANNNNNNNTTGG	6 cut(s)	14, 46, 209, 241, 2970, 3002
Alw21I	GWGCWC	2 cut(s)	862, 2544
Alw26I	GTCTC	6 cut(s)	149, 381, 1099, 2171, 2488, 2791
Alw44I	GTGCAC	1 cut(s)	858
AlwI	GGATC	4 cut(s)	34, 532, 1202, 2756
Aor13HI	TCCGGA	3 cut(s)	205, 780, 2793
ApaLI	GTGCAC	1 cut(s)	858
ApeKI	GCWGC	9 cut(s)	185, 671, 971, 1700, 1769, 1772, 2020, 2156, 2192
ApoI	RAATTY	5 cut(s)	253, 503, 736, 2300, 2808
ArsI	GACNNNNNNTTYG	2 cut(s)	1795, 1827
Asp700I	GAANNNNTTC	1 cut(s)	454
Asp718I	GGTACC	1 cut(s)	2901
AspLEI	GCGC	2 cut(s)	1191, 3035
AspS9I	GGNCC	7 cut(s)	53, 58, 615, 794, 927, 1811, 2423
AsuC2I	CCSGG	1 cut(s)	57
AvaII	GGWCC	3 cut(s)	53, 1811, 2423
AxyI	CCTNAGG	1 cut(s)	619
BaeGI	GKGCMC	1 cut(s)	862
BalI	TGGCCA	3 cut(s)	305, 585, 1969
BanI	GGYRCC	3 cut(s)	986, 2849, 2901
BanII	GRGCYC	1 cut(s)	2544
BbsI	GAAGAC	3 cut(s)	369, 2190, 2938
Bbv12I	GWGCWC	2 cut(s)	862, 2544
BbvI	GCAGC	9 cut(s)	172, 683, 958, 1712, 1781, 1784, 2032, 2143, 2179
BccI	CCATC	6 cut(s)	848, 1193, 1325, 1337, 2126, 2560
BceAI	ACGGC	3 cut(s)	87, 210, 1279
BciT130I	CCWGG	8 cut(s)	175, 223, 587, 613, 633, 1299, 1456, 2854
BciVI	GTATCC	1 cut(s)	777
BclI	TGATCA	2 cut(s)	138, 1864
BcnI	CCSGG	1 cut(s)	57
BcoDI	GTCTC	6 cut(s)	149, 381, 1099, 2171, 2488, 2791
BcuI	ACTAGT	2 cut(s)	482, 1849
BfaI	CTAG	9 cut(s)	429, 483, 1133, 1164, 1217, 1850, 2076, 2883, 3155
BfmI	CTRYAG	1 cut(s)	875
BfoI	RGCGCY	2 cut(s)	1192, 3036
BfrI	CTTAAG	2 cut(s)	1610, 3164
BfuAI	ACCTGC	2 cut(s)	950, 1257
BfuI	GTATCC	1 cut(s)	777
BglI	GCCNNNNNGGC	2 cut(s)	531, 2029
BlpI	GCTNAGC	1 cut(s)	294
Bme1390I	CCNGG	9 cut(s)	57, 175, 223, 587, 613, 633, 1299, 1456, 2854
Bme18I	GGWCC	3 cut(s)	53, 1811, 2423
BmgT120I	GGNCC	7 cut(s)	53, 58, 615, 794, 927, 1811, 2423
BmiI	GGNNCC	6 cut(s)	702, 988, 1812, 2263, 2851, 2903
BmrFI	CCNGG	9 cut(s)	57, 175, 223, 587, 613, 633, 1299, 1456, 2854
BmrI	ACTGGG	1 cut(s)	25
BmuI	ACTGGG	1 cut(s)	25
BoxI	GACNNNNGTC	4 cut(s)	51, 1487, 1842, 3090
BpiI	GAAGAC	3 cut(s)	369, 2190, 2938
BplI	GAGNNNNNCTC	2 cut(s)	2143, 2175
BpmI	CTGGAG	4 cut(s)	654, 660, 1281, 2217
Bpu1102I	GCTNAGC	1 cut(s)	294
BpuMI	CCSGG	1 cut(s)	57
BsaBI	GATNNNNATC	2 cut(s)	1151, 1824
BsaHI	GRCGYC	3 cut(s)	48, 69, 1063
BsaI	GGTCTC	2 cut(s)	149, 2488
BsaJI	CCNNGG	7 cut(s)	174, 541, 612, 797, 1427, 1455, 2015
BsaWI	WCCGGW	3 cut(s)	205, 780, 2793
BsaXI	ACNNNNNCTCC	4 cut(s)	221, 251, 378, 408
Bsc4I	CCNNNNNNNGG	9 cut(s)	175, 180, 1331, 1428, 1455, 1456, 1519, 2785, 2875
Bse1I	ACTGG	7 cut(s)	20, 482, 583, 920, 1018, 2004, 2133
Bse21I	CCTNAGG	1 cut(s)	619
Bse3DI	GCAATG	1 cut(s)	2737
Bse8I	GATNNNNATC	2 cut(s)	1151, 1824
BseAI	TCCGGA	3 cut(s)	205, 780, 2793
BseBI	CCWGG	8 cut(s)	175, 223, 587, 613, 633, 1299, 1456, 2854
BseDI	CCNNGG	7 cut(s)	174, 541, 612, 797, 1427, 1455, 2015
BseGI	GGATG	4 cut(s)	1348, 1888, 2137, 3122
BseJI	GATNNNNATC	2 cut(s)	1151, 1824
BseLI	CCNNNNNNNGG	9 cut(s)	175, 180, 1331, 1428, 1455, 1456, 1519, 2785, 2875
BseMI	GCAATG	1 cut(s)	2737
BseNI	ACTGG	7 cut(s)	20, 482, 583, 920, 1018, 2004, 2133
BseRI	GAGGAG	6 cut(s)	77, 214, 224, 447, 761, 1136
BseSI	GKGCMC	1 cut(s)	862
BseX3I	CGGCCG	1 cut(s)	100
BseXI	GCAGC	9 cut(s)	172, 683, 958, 1712, 1781, 1784, 2032, 2143, 2179
BseYI	CCCAGC	1 cut(s)	2631
Bsh1236I	CGCG	2 cut(s)	99, 1429
Bsh1285I	CGRYCG	2 cut(s)	103, 396
BshNI	GGYRCC	3 cut(s)	986, 2849, 2901
BsiEI	CGRYCG	2 cut(s)	103, 396
BsiHKAI	GWGCWC	2 cut(s)	862, 2544
BsiSI	CCGG	4 cut(s)	56, 206, 781, 2794
BslFI	GGGAC	2 cut(s)	983, 3078
BslI	CCNNNNNNNGG	9 cut(s)	175, 180, 1331, 1428, 1455, 1456, 1519, 2785, 2875
BsmAI	GTCTC	6 cut(s)	149, 381, 1099, 2171, 2488, 2791
BsmBI	CGTCTC	1 cut(s)	1099
BsmFI	GGGAC	2 cut(s)	983, 3078
BsmI	GAATGC	1 cut(s)	2309
Bso31I	GGTCTC	2 cut(s)	149, 2488
Bsp1286I	GDGCHC	2 cut(s)	862, 2544
Bsp13I	TCCGGA	3 cut(s)	205, 780, 2793
Bsp1720I	GCTNAGC	1 cut(s)	294
BspEI	TCCGGA	3 cut(s)	205, 780, 2793
BspFNI	CGCG	2 cut(s)	99, 1429
BspHI	TCATGA	1 cut(s)	2802
BspLI	GGNNCC	6 cut(s)	702, 988, 1812, 2263, 2851, 2903
BspMI	ACCTGC	2 cut(s)	950, 1257
BspPI	GGATC	4 cut(s)	34, 532, 1202, 2756
BspT107I	GGYRCC	3 cut(s)	986, 2849, 2901
BspTI	CTTAAG	2 cut(s)	1610, 3164
BspTNI	GGTCTC	2 cut(s)	149, 2488
BsrBI	CCGCTC	1 cut(s)	171
BsrDI	GCAATG	1 cut(s)	2737
BsrI	ACTGG	7 cut(s)	20, 482, 583, 920, 1018, 2004, 2133
BssECI	CCNNGG	7 cut(s)	174, 541, 612, 797, 1427, 1455, 2015
BssNI	GRCGYC	3 cut(s)	48, 69, 1063
BssT1I	CCWWGG	2 cut(s)	797, 2015
Bst2UI	CCWGG	8 cut(s)	175, 223, 587, 613, 633, 1299, 1456, 2854
Bst6I	CTCTTC	1 cut(s)	126
BstACI	GRCGYC	3 cut(s)	48, 69, 1063
BstAFI	CTTAAG	2 cut(s)	1610, 3164
BstC8I	GCNNGC	4 cut(s)	242, 262, 1931, 2984
BstDSI	CCRYGG	2 cut(s)	541, 1427
BstF5I	GGATG	4 cut(s)	1348, 1888, 2137, 3122
BstFNI	CGCG	2 cut(s)	99, 1429
BstH2I	RGCGCY	2 cut(s)	1192, 3036
BstHHI	GCGC	2 cut(s)	1191, 3035
BstMAI	GTCTC	6 cut(s)	149, 381, 1099, 2171, 2488, 2791
BstMCI	CGRYCG	2 cut(s)	103, 396
BstMWI	GCNNNNNNNGC	5 cut(s)	194, 531, 1263, 2029, 2992
BstNI	CCWGG	8 cut(s)	175, 223, 587, 613, 633, 1299, 1456, 2854
BstPAI	GACNNNNGTC	4 cut(s)	51, 1487, 1842, 3090
BstSCI	CCNGG	9 cut(s)	55, 173, 221, 585, 611, 631, 1297, 1454, 2852
BstSFI	CTRYAG	1 cut(s)	875
BstSLI	GKGCMC	1 cut(s)	862
BstUI	CGCG	2 cut(s)	99, 1429
BstV1I	GCAGC	9 cut(s)	172, 683, 958, 1712, 1781, 1784, 2032, 2143, 2179
BstV2I	GAAGAC	3 cut(s)	369, 2190, 2938
BstX2I	RGATCY	1 cut(s)	2761
BstXI	CCANNNNNNTGG	1 cut(s)	2195
BstYI	RGATCY	1 cut(s)	2761
BstZI	CGGCCG	1 cut(s)	100
Bsu36I	CCTNAGG	1 cut(s)	619
BsuI	GTATCC	1 cut(s)	777
BtgI	CCRYGG	2 cut(s)	541, 1427
BtsCI	GGATG	4 cut(s)	1348, 1888, 2137, 3122
BtsI	GCAGTG	2 cut(s)	474, 3034
BtsIMutI	CAGTG	7 cut(s)	474, 729, 1514, 2033, 2126, 2768, 3034
BveI	ACCTGC	2 cut(s)	950, 1257
Cac8I	GCNNGC	4 cut(s)	242, 262, 1931, 2984
CciI	TCATGA	1 cut(s)	2802
CfoI	GCGC	2 cut(s)	1191, 3035
Cfr13I	GGNCC	7 cut(s)	53, 58, 615, 794, 927, 1811, 2423
Cfr42I	CCGCGG	1 cut(s)	1430
CpoI	CGGWCCG	1 cut(s)	53
CseI	GACGC	2 cut(s)	77, 2715
CsiI	ACCWGGT	1 cut(s)	221
Csp6I	GTAC	2 cut(s)	1093, 2902
CspCI	CAANNNNNGTGG	2 cut(s)	648, 683
CspI	CGGWCCG	1 cut(s)	53
CviQI	GTAC	2 cut(s)	1093, 2902
DraIII	CACNNNGTG	1 cut(s)	2078
EaeI	YGGCCR	4 cut(s)	100, 303, 583, 1967
EagI	CGGCCG	1 cut(s)	100
Eam1104I	CTCTTC	1 cut(s)	126
EarI	CTCTTC	1 cut(s)	126
EciI	GGCGGA	1 cut(s)	259
Ecl136II	GAGCTC	1 cut(s)	2542
EclXI	CGGCCG	1 cut(s)	100
Eco130I	CCWWGG	2 cut(s)	797, 2015
Eco24I	GRGCYC	1 cut(s)	2544
Eco31I	GGTCTC	2 cut(s)	149, 2488
Eco47I	GGWCC	3 cut(s)	53, 1811, 2423
Eco52I	CGGCCG	1 cut(s)	100
Eco53kI	GAGCTC	1 cut(s)	2542
Eco57I	CTGAAG	4 cut(s)	1913, 2166, 2202, 3035
Eco81I	CCTNAGG	1 cut(s)	619
EcoICRI	GAGCTC	1 cut(s)	2542
EcoO109I	RGGNCCY	3 cut(s)	615, 1811, 2423
EcoRI	GAATTC	2 cut(s)	736, 2808
EcoRII	CCWGG	8 cut(s)	173, 221, 585, 611, 631, 1297, 1454, 2852
EcoT14I	CCWWGG	2 cut(s)	797, 2015
EcoT22I	ATGCAT	1 cut(s)	1591
EcoT38I	GRGCYC	1 cut(s)	2544
ErhI	CCWWGG	2 cut(s)	797, 2015
Esp3I	CGTCTC	1 cut(s)	1099
FalI	AAGNNNNNCTT	6 cut(s)	810, 842, 1973, 2005, 2970, 3002
FaqI	GGGAC	2 cut(s)	983, 3078
FbaI	TGATCA	2 cut(s)	138, 1864
FblI	GTMKAC	1 cut(s)	1846
FokI	GGATG	4 cut(s)	1355, 1895, 2144, 3129
FriOI	GRGCYC	1 cut(s)	2544
FspBI	CTAG	9 cut(s)	429, 483, 1133, 1164, 1217, 1850, 2076, 2883, 3155
GlaI	GCGC	2 cut(s)	1190, 3034
GsaI	CCCAGC	1 cut(s)	2635
GsuI	CTGGAG	4 cut(s)	654, 660, 1281, 2217
HaeII	RGCGCY	2 cut(s)	1192, 3036
HapII	CCGG	4 cut(s)	56, 206, 781, 2794
HgaI	GACGC	2 cut(s)	77, 2715
HhaI	GCGC	2 cut(s)	1191, 3035
Hin1I	GRCGYC	3 cut(s)	48, 69, 1063
Hin6I	GCGC	2 cut(s)	1189, 3033
HinP1I	GCGC	2 cut(s)	1189, 3033
HincII	GTYRAC	5 cut(s)	488, 1128, 1855, 2730, 2934
HindII	GTYRAC	5 cut(s)	488, 1128, 1855, 2730, 2934
HindIII	AAGCTT	3 cut(s)	1037, 1895, 3041
HpaI	GTTAAC	1 cut(s)	1128
HpaII	CCGG	4 cut(s)	56, 206, 781, 2794
Hpy99I	CGWCG	6 cut(s)	53, 71, 82, 131, 196, 1340
HpyCH4IV	ACGT	5 cut(s)	48, 1063, 1091, 2657, 2829
HpyCH4V	TGCA	9 cut(s)	467, 860, 935, 953, 959, 1589, 1929, 2010, 2420
HpyF10VI	GCNNNNNNNGC	5 cut(s)	194, 531, 1263, 2029, 2992
HpySE526I	ACGT	5 cut(s)	48, 1063, 1091, 2657, 2829
Hsp92I	GRCGYC	3 cut(s)	48, 69, 1063
HspAI	GCGC	2 cut(s)	1189, 3033
Kpn2I	TCCGGA	3 cut(s)	205, 780, 2793
KpnI	GGTACC	1 cut(s)	2905
Ksp22I	TGATCA	2 cut(s)	138, 1864
KspAI	GTTAAC	1 cut(s)	1128
KspI	CCGCGG	1 cut(s)	1430
LmnI	GCTCC	8 cut(s)	176, 556, 679, 748, 767, 2212, 2636, 3125
Lsp1109I	GCAGC	9 cut(s)	172, 683, 958, 1712, 1781, 1784, 2032, 2143, 2179
MabI	ACCWGGT	1 cut(s)	221
MaeI	CTAG	9 cut(s)	429, 483, 1133, 1164, 1217, 1850, 2076, 2883, 3155
MaeII	ACGT	5 cut(s)	48, 1063, 1091, 2657, 2829
MaeIII	GTNAC	9 cut(s)	26, 119, 398, 1016, 1259, 2389, 2525, 2707, 2963
MbiI	CCGCTC	1 cut(s)	171
MflI	RGATCY	1 cut(s)	2761
MhlI	GDGCHC	2 cut(s)	862, 2544
MlsI	TGGCCA	3 cut(s)	305, 585, 1969
MluNI	TGGCCA	3 cut(s)	305, 585, 1969
MlyI	GAGTC	4 cut(s)	109, 211, 2506, 2793
MmeI	TCCRAC	3 cut(s)	137, 207, 1380
Mox20I	TGGCCA	3 cut(s)	305, 585, 1969
Mph1103I	ATGCAT	1 cut(s)	1591
MroI	TCCGGA	3 cut(s)	205, 780, 2793
MroXI	GAANNNNTTC	1 cut(s)	454
MscI	TGGCCA	3 cut(s)	305, 585, 1969
MseI	TTAA	9 cut(s)	518, 725, 1127, 1611, 1760, 1991, 2208, 2784, 3165
MslI	CAYNNNNRTG	2 cut(s)	417, 2742
Msp20I	TGGCCA	3 cut(s)	305, 585, 1969
MspA1I	CMGCKG	3 cut(s)	1429, 2532, 2631
MspCI	CTTAAG	2 cut(s)	1610, 3164
MspI	CCGG	4 cut(s)	56, 206, 781, 2794
MspR9I	CCNGG	9 cut(s)	57, 175, 223, 587, 613, 633, 1299, 1456, 2854
Mva1269I	GAATGC	1 cut(s)	2309
MvaI	CCWGG	8 cut(s)	175, 223, 587, 613, 633, 1299, 1456, 2854
MvnI	CGCG	2 cut(s)	99, 1429
MwoI	GCNNNNNNNGC	5 cut(s)	194, 531, 1263, 2029, 2992
NciI	CCSGG	1 cut(s)	57
NlaIV	GGNNCC	6 cut(s)	702, 988, 1812, 2263, 2851, 2903
NmuCI	GTSAC	3 cut(s)	398, 1016, 2963
NsiI	ATGCAT	1 cut(s)	1591
PagI	TCATGA	1 cut(s)	2802
PaqCI	CACCTGC	1 cut(s)	950
PcsI	WCGNNNNNNNCGW	1 cut(s)	156
PctI	GAATGC	1 cut(s)	2309
PdmI	GAANNNNTTC	1 cut(s)	454
PflFI	GACNNNGTC	3 cut(s)	312, 392, 2233
PflMI	CCANNNNNTGG	1 cut(s)	1331
PfoI	TCCNGGA	1 cut(s)	1297
PleI	GAGTC	4 cut(s)	109, 210, 2505, 2793
PpsI	GAGTC	4 cut(s)	109, 210, 2505, 2793
PpuMI	RGGWCCY	2 cut(s)	1811, 2423
PshAI	GACNNNNGTC	4 cut(s)	51, 1487, 1842, 3090
Psp124BI	GAGCTC	1 cut(s)	2544
Psp5II	RGGWCCY	2 cut(s)	1811, 2423
Psp6I	CCWGG	8 cut(s)	173, 221, 585, 611, 631, 1297, 1454, 2852
PspFI	CCCAGC	1 cut(s)	2631
PspGI	CCWGG	8 cut(s)	173, 221, 585, 611, 631, 1297, 1454, 2852
PspN4I	GGNNCC	6 cut(s)	702, 988, 1812, 2263, 2851, 2903
PspPI	GGNCC	7 cut(s)	53, 58, 615, 794, 927, 1811, 2423
PspPPI	RGGWCCY	2 cut(s)	1811, 2423
PsuI	RGATCY	1 cut(s)	2761
PsyI	GACNNNGTC	3 cut(s)	312, 392, 2233
RsaI	GTAC	2 cut(s)	1094, 2903
RsaNI	GTAC	2 cut(s)	1093, 2902
RseI	CAYNNNNRTG	2 cut(s)	417, 2742
Rsr2I	CGGWCCG	1 cut(s)	53
RsrII	CGGWCCG	1 cut(s)	53
SacI	GAGCTC	1 cut(s)	2544
SacII	CCGCGG	1 cut(s)	1430
SaqAI	TTAA	9 cut(s)	518, 725, 1127, 1611, 1760, 1991, 2208, 2784, 3165
Sau96I	GGNCC	7 cut(s)	53, 58, 615, 794, 927, 1811, 2423
SchI	GAGTC	4 cut(s)	109, 211, 2506, 2793
ScrFI	CCNGG	9 cut(s)	57, 175, 223, 587, 613, 633, 1299, 1456, 2854
SduI	GDGCHC	2 cut(s)	862, 2544
SexAI	ACCWGGT	1 cut(s)	221
SfcI	CTRYAG	1 cut(s)	875
Sfr303I	CCGCGG	1 cut(s)	1430
SgrBI	CCGCGG	1 cut(s)	1430
SinI	GGWCC	3 cut(s)	53, 1811, 2423
SmiMI	CAYNNNNRTG	2 cut(s)	417, 2742
SmlI	CTYRAG	2 cut(s)	1610, 3164
SmoI	CTYRAG	2 cut(s)	1610, 3164
SpeI	ACTAGT	2 cut(s)	482, 1849
SspI	AATATT	1 cut(s)	1477
SspMI	CTAG	9 cut(s)	429, 483, 1133, 1164, 1217, 1850, 2076, 2883, 3155
SstI	GAGCTC	1 cut(s)	2544
StyD4I	CCNGG	9 cut(s)	55, 173, 221, 585, 611, 631, 1297, 1454, 2852
StyI	CCWWGG	2 cut(s)	797, 2015
TaiI	ACGT	5 cut(s)	51, 1066, 1094, 2660, 2832
TaqI	TCGA	2 cut(s)	129, 1740
TaqII	GACCGA	2 cut(s)	41, 173
TauI	GCSGC	2 cut(s)	102, 191
Tru1I	TTAA	9 cut(s)	518, 725, 1127, 1611, 1760, 1991, 2208, 2784, 3165
Tru9I	TTAA	9 cut(s)	518, 725, 1127, 1611, 1760, 1991, 2208, 2784, 3165
TscAI	CASTG	7 cut(s)	474, 736, 1521, 2033, 2133, 2775, 3034
TseFI	GTSAC	3 cut(s)	398, 1016, 2963
TseI	GCWGC	9 cut(s)	185, 671, 971, 1700, 1769, 1772, 2020, 2156, 2192
Tsp45I	GTSAC	3 cut(s)	398, 1016, 2963
TspGWI	ACGGA	6 cut(s)	80, 530, 1123, 2810, 2903, 2984
TspRI	CASTG	7 cut(s)	474, 736, 1521, 2033, 2133, 2775, 3034
Tth111I	GACNNNGTC	3 cut(s)	312, 392, 2233
Van91I	CCANNNNNTGG	1 cut(s)	1331
Vha464I	CTTAAG	2 cut(s)	1610, 3164
VneI	GTGCAC	1 cut(s)	858
VpaK11BI	GGWCC	3 cut(s)	53, 1811, 2423
XapI	RAATTY	5 cut(s)	253, 503, 736, 2300, 2808
XbaI	TCTAGA	1 cut(s)	2882
XcmI	CCANNNNNNNNNTGG	1 cut(s)	684
XmiI	GTMKAC	1 cut(s)	1846
XmnI	GAANNNNTTC	1 cut(s)	454
XspI	CTAG	9 cut(s)	429, 483, 1133, 1164, 1217, 1850, 2076, 2883, 3155
ZraI	GACGTC	2 cut(s)	49, 1064
Zsp2I	ATGCAT	1 cut(s)	1591

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.