FvH4_2g32170

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013254
Physical Location & Seq
Forward (+)
24453074 .. 24455098
2025 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32170.t1

Sequence Viewer

Length: 1011 bp
ATGGGAATCTGCATATCCTGCTCAGCTTCTCAAAAGATTCAAGATGGCAATGAAAATGCAATGTTCTTTGGAAAGAACAGTAGTTTCTTTGGGGATCAGAGCCTTGGTTCTCTTTATTCTAGGGCAGGAAGCAAAGGACTAAACCAAGACAGTGCCATTCTTTACCAGGGCTATGGAGTTGGAGGTGCATCTTTCTGTGGAGTTTTTGATGGGCATGGAAAGAATGGTCATGTAGTGAGTGAGATGGTGAAAAATCGCCTACCAGCACTACTACAAAGCCAAATGGAAGCTCTTGAACAAGTTACTCTAGTAACAAAATCAGATGGTGAACAAAATTGCAACAACGAAACAGATGCTGAAGCAGAACCAAGCAAGAACTTTGCTAAGTGGGAAGCGGCACTTGTTAATGCCTTCAGAGTAATGGACAAGGAGATTAAGCTACAACAAGATTTGGACTGCTCTTCTAGTGGAAGCACTGCTGTTGTTGTTATAAGACAGGGTGAGGACCTTTTTATAGCTAATCTGGGTGACTCAAGAGCCGTCCTGGGAACAATGACTGAGAATGGAATTGAAGGCATTCAATTAACCACTGATCTAAAGCCTGGTTTACCAAGTGAAACTGAACGAATAACAGCATGCAACGGTCGAGTGTTTGCACTGAAGAACGAAAAACACATCCAGAGAGTGTGGTTGCCCCATGAGCTCCTTCCAGGTCTAGCCATGTCGCGAGCTTTTGGGGACTTTGTACTCAAAGATTATGGCATCATTGCCATCCCTGATGTCTCCCATCGTCGCCTGACCTCCAAAGATCAGTTCATTGTTCTTGCTACTGATGGGATATGGGATGTGCTTAGTAACAATGAAGTTGCATCTATTGTGTGGGAAGCCAAAAGTGCAGAGGCAGCTGCTAGGAGAGTGACTGAAACAGCTATAGCCACATGGAGCACGAAGTACCCAACTACTAAGATAGACGACTGCACCGTGGTGTGCCTGTTTTTCAGTTCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

337

Amino Acids

36.46

Weight (kDa)

5.09

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C_2 PF13672 47 - 291 2.2e-06 Protein phosphatase 2C
PP2C PF00481 109 - 315 7.1e-44 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26010
fragaria_vesca FvH4_2g32170
malus_domestica MD08G1073400.v1.1
prunus_persica Prupe.1G414800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310511
rosa_laevigata RLG00000010477
rosa_multiflora Rmu_sc0016392.1_g000006 Rmu_sc0020729.1_g000003 Rmu_sc0035020.1_g000002
rosa_roxburghii Rroxscaffold_7G00158470
rosa_rugosa Rorug06G0380900 Rorug06G0381000 Rorug06G0381100
rosa_samantha Rh6AG495000 Rh6BG504600 Rh6CG509900 Rh6DG495900
rosa_wichuraiana Rw6G043050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 491
AccII CGCG 1 cut(s) 727
AciI CCGC 1 cut(s) 395
AclWI GGATC 1 cut(s) 102
AcuI CTGAAG 3 cut(s) 378, 397, 680
AfaI GTAC 2 cut(s) 747, 953
AgsI TTSAA 4 cut(s) 41, 296, 572, 581
AjnI CCWGG 4 cut(s) 165, 543, 601, 709
AjuI GAANNNNNNNTTGG 2 cut(s) 797, 829
AleI CACNNNNGTG 1 cut(s) 983
AluBI AGCT 8 cut(s) 26, 290, 439, 518, 703, 731, 905, 929
AluI AGCT 8 cut(s) 26, 290, 439, 518, 703, 731, 905, 929
Alw21I GWGCWC 2 cut(s) 705, 947
Alw26I GTCTC 1 cut(s) 787
AlwI GGATC 1 cut(s) 102
AlwNI CAGNNNCTG 1 cut(s) 356
ApeKI GCWGC 2 cut(s) 902, 905
Asp700I GAANNNNTTC 1 cut(s) 576
AspS9I GGNCC 1 cut(s) 505
AsuHPI GGTGA 4 cut(s) 259, 338, 512, 539
AvaII GGWCC 1 cut(s) 505
BanII GRGCYC 1 cut(s) 705
Bbv12I GWGCWC 2 cut(s) 705, 947
BbvI GCAGC 2 cut(s) 892, 914
BccI CCATC 7 cut(s) 38, 203, 238, 317, 779, 795, 827
BceAI ACGGC 1 cut(s) 524
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BciT130I CCWGG 4 cut(s) 167, 545, 603, 711
BcoDI GTCTC 1 cut(s) 787
BfaI CTAG 5 cut(s) 120, 308, 465, 716, 909
BfmI CTRYAG 1 cut(s) 930
BisI GCNGC 3 cut(s) 396, 903, 906
BlpI GCTNAGC 1 cut(s) 22
BlsI GCNGC 3 cut(s) 397, 904, 907
Bme1390I CCNGG 4 cut(s) 167, 545, 603, 711
Bme18I GGWCC 1 cut(s) 505
BmgT120I GGNCC 1 cut(s) 505
BmrFI CCNGG 4 cut(s) 167, 545, 603, 711
BmsI GCATC 4 cut(s) 197, 343, 771, 878
Bpu1102I GCTNAGC 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 517
BsaJI CCNNGG 4 cut(s) 103, 166, 544, 981
Bse3DI GCAATG 3 cut(s) 55, 66, 765
BseBI CCWGG 4 cut(s) 167, 545, 603, 711
BseDI CCNNGG 4 cut(s) 103, 166, 544, 981
BseGI GGATG 3 cut(s) 675, 771, 850
BseMI GCAATG 3 cut(s) 55, 66, 765
BseMII CTCAG 2 cut(s) 36, 549
BseXI GCAGC 2 cut(s) 892, 914
BsgI GTGCAG 2 cut(s) 915, 961
Bsh1236I CGCG 1 cut(s) 727
Bsh1285I CGRYCG 1 cut(s) 646
BsiEI CGRYCG 1 cut(s) 646
BsiHKAI GWGCWC 2 cut(s) 705, 947
BslFI GGGAC 1 cut(s) 752
BsmAI GTCTC 1 cut(s) 787
BsmFI GGGAC 1 cut(s) 752
BsmI GAATGC 1 cut(s) 576
Bsp1286I GDGCHC 2 cut(s) 705, 947
Bsp143I GATC 3 cut(s) 94, 592, 808
Bsp1720I GCTNAGC 1 cut(s) 22
Bsp68I TCGCGA 1 cut(s) 727
BspACI CCGC 1 cut(s) 395
BspCNI CTCAG 2 cut(s) 35, 550
BspFNI CGCG 1 cut(s) 727
BspPI GGATC 1 cut(s) 102
BspQI GCTCTTC 1 cut(s) 466
BsrDI GCAATG 3 cut(s) 55, 66, 765
BssECI CCNNGG 4 cut(s) 103, 166, 544, 981
BssMI GATC 3 cut(s) 94, 592, 808
BssT1I CCWWGG 1 cut(s) 103
Bst2UI CCWGG 4 cut(s) 167, 545, 603, 711
Bst4CI ACNGT 4 cut(s) 80, 152, 644, 982
Bst6I CTCTTC 1 cut(s) 466
BstAPI GCANNNNNTGC 1 cut(s) 18
BstC8I GCNNGC 2 cut(s) 637, 729
BstDEI CTNAG 5 cut(s) 22, 384, 558, 851, 963
BstDSI CCRYGG 1 cut(s) 981
BstF5I GGATG 3 cut(s) 675, 771, 850
BstFNI CGCG 1 cut(s) 727
BstKTI GATC 3 cut(s) 97, 595, 811
BstMAI GTCTC 1 cut(s) 787
BstMBI GATC 3 cut(s) 94, 592, 808
BstMCI CGRYCG 1 cut(s) 646
BstMWI GCNNNNNNNGC 4 cut(s) 18, 700, 893, 902
BstNI CCWGG 4 cut(s) 167, 545, 603, 711
BstNSI RCATGY 1 cut(s) 639
BstSCI CCNGG 4 cut(s) 165, 543, 601, 709
BstSFI CTRYAG 1 cut(s) 930
BstUI CGCG 1 cut(s) 727
BstV1I GCAGC 2 cut(s) 892, 914
BstXI CCANNNNNNTGG 1 cut(s) 173
BtgI CCRYGG 1 cut(s) 981
BtsCI GGATG 3 cut(s) 675, 771, 850
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 4 cut(s) 157, 474, 588, 656
BtuMI TCGCGA 1 cut(s) 727
Cac8I GCNNGC 2 cut(s) 637, 729
CaiI CAGNNNCTG 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 505
Csp6I GTAC 2 cut(s) 746, 952
CviAII CATG 6 cut(s) 215, 230, 636, 698, 721, 939
CviQI GTAC 2 cut(s) 746, 952
DdeI CTNAG 5 cut(s) 22, 384, 558, 851, 963
DpnI GATC 3 cut(s) 96, 594, 810
DpnII GATC 3 cut(s) 94, 592, 808
Eam1104I CTCTTC 1 cut(s) 466
EarI CTCTTC 1 cut(s) 466
Ecl136II GAGCTC 1 cut(s) 703
Eco130I CCWWGG 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 705
Eco47I GGWCC 1 cut(s) 505
Eco53kI GAGCTC 1 cut(s) 703
Eco57I CTGAAG 3 cut(s) 378, 397, 680
EcoICRI GAGCTC 1 cut(s) 703
EcoO109I RGGNCCY 1 cut(s) 505
EcoRII CCWGG 4 cut(s) 165, 543, 601, 709
EcoT14I CCWWGG 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 705
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 6 cut(s) 218, 233, 639, 701, 724, 942
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 1 cut(s) 752
FatI CATG 6 cut(s) 214, 229, 635, 697, 720, 938
Fnu4HI GCNGC 3 cut(s) 396, 903, 906
FokI GGATG 3 cut(s) 662, 758, 857
FriOI GRGCYC 1 cut(s) 705
Fsp4HI GCNGC 3 cut(s) 396, 903, 906
FspBI CTAG 5 cut(s) 120, 308, 465, 716, 909
GluI GCNGC 3 cut(s) 396, 903, 906
Hin1II CATG 6 cut(s) 218, 233, 639, 701, 724, 942
HinfI GANTC 3 cut(s) 6, 37, 530
HphI GGTGA 4 cut(s) 259, 338, 512, 539
Hpy166II GTNNAC 2 cut(s) 329, 608
Hpy188I TCNGA 3 cut(s) 99, 322, 416
Hpy188III TCNNGA 5 cut(s) 41, 293, 534, 679, 726
Hpy8I GTNNAC 2 cut(s) 329, 608
Hpy99I CGWCG 1 cut(s) 795
HpyAV CCTTC 3 cut(s) 421, 566, 716
HpyCH4III ACNGT 4 cut(s) 80, 152, 644, 982
HpyCH4V TGCA 9 cut(s) 12, 59, 188, 339, 639, 656, 869, 896, 978
HpyF10VI GCNNNNNNNGC 4 cut(s) 18, 700, 893, 902
HpyF3I CTNAG 5 cut(s) 22, 384, 558, 851, 963
Hsp92II CATG 6 cut(s) 218, 233, 639, 701, 724, 942
Kzo9I GATC 3 cut(s) 94, 592, 808
LguI GCTCTTC 1 cut(s) 466
LmnI GCTCC 2 cut(s) 708, 942
Lsp1109I GCAGC 2 cut(s) 892, 914
LweI GCATC 4 cut(s) 197, 343, 771, 878
MaeI CTAG 5 cut(s) 120, 308, 465, 716, 909
MaeIII GTNAC 5 cut(s) 301, 310, 527, 854, 916
MalI GATC 3 cut(s) 96, 594, 810
MboI GATC 3 cut(s) 94, 592, 808
MboII GAAGA 2 cut(s) 453, 673
MhlI GDGCHC 2 cut(s) 705, 947
MluCI AATT 3 cut(s) 334, 567, 581
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 4 cut(s) 176, 496, 811, 892
MroXI GAANNNNTTC 1 cut(s) 576
MseI TTAA 3 cut(s) 405, 435, 584
MslI CAYNNNNRTG 1 cut(s) 983
MspA1I CMGCKG 1 cut(s) 905
MspR9I CCNGG 4 cut(s) 167, 545, 603, 711
Mva1269I GAATGC 1 cut(s) 576
MvaI CCWGG 4 cut(s) 167, 545, 603, 711
MvnI CGCG 1 cut(s) 727
MwoI GCNNNNNNNGC 4 cut(s) 18, 700, 893, 902
NdeII GATC 3 cut(s) 94, 592, 808
NlaIII CATG 6 cut(s) 218, 233, 639, 701, 724, 942
NmuCI GTSAC 2 cut(s) 527, 916
NruI TCGCGA 1 cut(s) 727
NspI RCATGY 1 cut(s) 639
OliI CACNNNNGTG 1 cut(s) 983
PaeI GCATGC 1 cut(s) 639
PciSI GCTCTTC 1 cut(s) 466
PcsI WCGNNNNNNNCGW 1 cut(s) 978
PctI GAATGC 1 cut(s) 576
PdmI GAANNNNTTC 1 cut(s) 576
PfeI GAWTC 2 cut(s) 6, 37
PkrI GCNGC 3 cut(s) 397, 904, 907
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
PpuMI RGGWCCY 1 cut(s) 505
PsiI TTATAA 1 cut(s) 491
Psp124BI GAGCTC 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 505
Psp6I CCWGG 4 cut(s) 165, 543, 601, 709
PspGI CCWGG 4 cut(s) 165, 543, 601, 709
PspPI GGNCC 1 cut(s) 505
PspPPI RGGWCCY 1 cut(s) 505
PstNI CAGNNNCTG 1 cut(s) 356
PvuII CAGCTG 1 cut(s) 905
RruI TCGCGA 1 cut(s) 727
RsaI GTAC 2 cut(s) 747, 953
RsaNI GTAC 2 cut(s) 746, 952
RseI CAYNNNNRTG 1 cut(s) 983
SacI GAGCTC 1 cut(s) 705
SapI GCTCTTC 1 cut(s) 466
SaqAI TTAA 3 cut(s) 405, 435, 584
SatI GCNGC 3 cut(s) 396, 903, 906
Sau3AI GATC 3 cut(s) 94, 592, 808
Sau96I GGNCC 1 cut(s) 505
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 4 cut(s) 167, 545, 603, 711
SduI GDGCHC 2 cut(s) 705, 947
SfaNI GCATC 4 cut(s) 197, 343, 771, 878
SfcI CTRYAG 1 cut(s) 930
SinI GGWCC 1 cut(s) 505
SmiMI CAYNNNNRTG 1 cut(s) 983
SmlI CTYRAG 1 cut(s) 532
SmoI CTYRAG 1 cut(s) 532
SphI GCATGC 1 cut(s) 639
Sse9I AATT 3 cut(s) 334, 567, 581
SsiI CCGC 1 cut(s) 395
SspMI CTAG 5 cut(s) 120, 308, 465, 716, 909
SstI GAGCTC 1 cut(s) 705
StyD4I CCNGG 4 cut(s) 165, 543, 601, 709
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 4 cut(s) 80, 152, 644, 982
TaqI TCGA 1 cut(s) 646
TasI AATT 3 cut(s) 334, 567, 581
TatI WGTACW 1 cut(s) 745
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 2 cut(s) 6, 37
Tru1I TTAA 3 cut(s) 405, 435, 584
Tru9I TTAA 3 cut(s) 405, 435, 584
TscAI CASTG 4 cut(s) 157, 481, 595, 663
TseFI GTSAC 2 cut(s) 527, 916
TseI GCWGC 2 cut(s) 902, 905
Tsp45I GTSAC 2 cut(s) 527, 916
TspDTI ATGAA 4 cut(s) 66, 805, 876, 993
TspRI CASTG 4 cut(s) 157, 481, 595, 663
VpaK11BI GGWCC 1 cut(s) 505
XceI RCATGY 1 cut(s) 639
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 5 cut(s) 120, 308, 465, 716, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.