Rw6G043050

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0013254
Physical Location & Seq
Reverse (-)
66454540 .. 66455591
1052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G043050.1

Sequence Viewer

Length: 702 bp
ATGGGAATCTGCATATCCTGCTCAGCGTCAGGTCAAAAGATTCAAGATGGCAATGAAAATGCAATGTTCTTTGGAAAGAACAGTGGTTCTTTTGGGACTCAGAGCCTTGGTTCGCTTGATTCTAAGGAAGGAAGCAAAGGACTAAACCAAGACAGTGCCATTCTTTACCAGGGCTATGGAGTTGGAGATTCATCTTTCTCTAACTTGGGTGACTCGAGAGCCATCCTGGGAACAATGACTGAGAATGGAATTGAAGGCATTCAATTAACCACTGATCTAAAGCCTGGTTTACCAAATGAAGCAGAACGAATAAGAGCATGCAACGGTCGAGTGATTGCACTAGAACACGAAAAACACATCCAGAGAGTGTGGTTGCCCCATGAATTCCTTCCAGGCCTAGCCATGTCCCGAGCTTTTGGGGACTTTGTACTCAAAGATTATGGCATCATTGCCGCCCCTGATGTCTCCCATCATCGCCTGACCTCCGAAGATCAGTTCATTGTTCTTGCAACTGATGGGGTATGGGATGTGCTTAGTAACAATGAAGTTGCGTCTATTGTGTGGGAAGCCGAGAGTGCAGAGGCAGCTGCTAGGACTGTGACTGAGACGGCTAGAGCCACATGGAGCACAAAATACCCAACTTCTAAGATAGACGACTGCACTGTGGTTTGCCTGTTTTTCAGTTCATTCCAATGCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

233

Amino Acids

25.17

Weight (kDa)

4.84

Isoelectric Point (pI)

30.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 66 - 206 8e-32 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26010
fragaria_vesca FvH4_2g32170
malus_domestica MD08G1073400.v1.1
prunus_persica Prupe.1G414800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310511
rosa_laevigata RLG00000010477
rosa_multiflora Rmu_sc0016392.1_g000006 Rmu_sc0020729.1_g000003 Rmu_sc0035020.1_g000002
rosa_roxburghii Rroxscaffold_7G00158470
rosa_rugosa Rorug06G0380900 Rorug06G0381000 Rorug06G0381100
rosa_samantha Rh6AG495000 Rh6BG504600 Rh6CG509900 Rh6DG495900
rosa_wichuraiana Rw6G043050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 453
AcsI RAATTY 1 cut(s) 383
AfaI GTAC 1 cut(s) 429
AgsI TTSAA 3 cut(s) 44, 254, 263
AjnI CCWGG 4 cut(s) 168, 225, 283, 391
AluBI AGCT 2 cut(s) 413, 587
AluI AGCT 2 cut(s) 413, 587
Alw21I GWGCWC 1 cut(s) 629
Alw26I GTCTC 2 cut(s) 469, 599
Ama87I CYCGRG 2 cut(s) 214, 408
AoxI GGCC 1 cut(s) 394
ApeKI GCWGC 2 cut(s) 584, 587
ApoI RAATTY 1 cut(s) 383
Asp700I GAANNNNTTC 2 cut(s) 258, 387
AsuHPI GGTGA 1 cut(s) 221
AvaI CYCGRG 2 cut(s) 214, 408
Bbv12I GWGCWC 1 cut(s) 629
BbvI GCAGC 2 cut(s) 574, 596
BccI CCATC 4 cut(s) 41, 230, 477, 509
BceAI ACGGC 1 cut(s) 624
BciT130I CCWGG 4 cut(s) 170, 227, 285, 393
BcoDI GTCTC 2 cut(s) 469, 599
BfaI CTAG 4 cut(s) 341, 398, 591, 612
BisI GCNGC 3 cut(s) 453, 585, 588
BlpI GCTNAGC 1 cut(s) 22
BlsI GCNGC 3 cut(s) 454, 586, 589
Bme1390I CCNGG 4 cut(s) 170, 227, 285, 393
BmeT110I CYCGRG 2 cut(s) 214, 408
BmrFI CCNGG 4 cut(s) 170, 227, 285, 393
BmsI GCATC 1 cut(s) 453
Bpu1102I GCTNAGC 1 cut(s) 22
BsaJI CCNNGG 3 cut(s) 106, 169, 226
Bse3DI GCAATG 3 cut(s) 58, 69, 447
BseBI CCWGG 4 cut(s) 170, 227, 285, 393
BseDI CCNNGG 3 cut(s) 106, 169, 226
BseGI GGATG 3 cut(s) 222, 357, 532
BseMI GCAATG 3 cut(s) 58, 69, 447
BseMII CTCAG 4 cut(s) 36, 113, 231, 594
BseXI GCAGC 2 cut(s) 574, 596
BsgI GTGCAG 2 cut(s) 597, 643
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 1 cut(s) 396
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 629
BsiHKCI CYCGRG 2 cut(s) 214, 408
BslFI GGGAC 3 cut(s) 109, 391, 434
BsmAI GTCTC 2 cut(s) 469, 599
BsmBI CGTCTC 1 cut(s) 599
BsmFI GGGAC 3 cut(s) 109, 391, 434
BsmI GAATGC 1 cut(s) 258
BsnI GGCC 1 cut(s) 396
BsoBI CYCGRG 2 cut(s) 214, 408
Bsp1286I GDGCHC 1 cut(s) 629
Bsp143I GATC 2 cut(s) 274, 490
Bsp1720I GCTNAGC 1 cut(s) 22
BspACI CCGC 1 cut(s) 453
BspANI GGCC 1 cut(s) 396
BspCNI CTCAG 4 cut(s) 35, 112, 232, 595
BsrDI GCAATG 3 cut(s) 58, 69, 447
BssECI CCNNGG 3 cut(s) 106, 169, 226
BssMI GATC 2 cut(s) 274, 490
BssT1I CCWWGG 1 cut(s) 106
Bst2UI CCWGG 4 cut(s) 170, 227, 285, 393
Bst4CI ACNGT 5 cut(s) 83, 155, 326, 598, 664
BstAPI GCANNNNNTGC 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 319
BstDEI CTNAG 7 cut(s) 22, 99, 123, 240, 533, 603, 645
BstF5I GGATG 3 cut(s) 222, 357, 532
BstKTI GATC 2 cut(s) 277, 493
BstMAI GTCTC 2 cut(s) 469, 599
BstMBI GATC 2 cut(s) 274, 490
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 3 cut(s) 18, 575, 584
BstNI CCWGG 4 cut(s) 170, 227, 285, 393
BstNSI RCATGY 1 cut(s) 321
BstSCI CCNGG 4 cut(s) 168, 225, 283, 391
BstV1I GCAGC 2 cut(s) 574, 596
BstXI CCANNNNNNTGG 1 cut(s) 176
BsuRI GGCC 1 cut(s) 396
BtgZI GCGATG 1 cut(s) 458
BtsCI GGATG 3 cut(s) 222, 357, 532
BtsIMutI CAGTG 4 cut(s) 88, 160, 270, 660
Cac8I GCNNGC 1 cut(s) 319
CseI GACGC 2 cut(s) 15, 540
Csp6I GTAC 1 cut(s) 428
CviAII CATG 4 cut(s) 318, 380, 403, 621
CviQI GTAC 1 cut(s) 428
DdeI CTNAG 7 cut(s) 22, 99, 123, 240, 533, 603, 645
DpnI GATC 2 cut(s) 276, 492
DpnII GATC 2 cut(s) 274, 490
Eco130I CCWWGG 1 cut(s) 106
Eco147I AGGCCT 1 cut(s) 396
Eco88I CYCGRG 2 cut(s) 214, 408
EcoRI GAATTC 1 cut(s) 383
EcoRII CCWGG 4 cut(s) 168, 225, 283, 391
EcoT14I CCWWGG 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 106
Esp3I CGTCTC 1 cut(s) 599
FaeI CATG 4 cut(s) 321, 383, 406, 624
FaiI YATR 8 cut(s) 14, 177, 319, 381, 404, 441, 523, 622
FaqI GGGAC 3 cut(s) 109, 391, 434
FatI CATG 4 cut(s) 317, 379, 402, 620
Fnu4HI GCNGC 3 cut(s) 453, 585, 588
FokI GGATG 3 cut(s) 209, 344, 539
Fsp4HI GCNGC 3 cut(s) 453, 585, 588
FspBI CTAG 4 cut(s) 341, 398, 591, 612
GluI GCNGC 3 cut(s) 453, 585, 588
HaeIII GGCC 1 cut(s) 396
HgaI GACGC 2 cut(s) 15, 540
Hin1II CATG 4 cut(s) 321, 383, 406, 624
HinfI GANTC 6 cut(s) 6, 40, 97, 119, 188, 212
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 1 cut(s) 290
Hpy188I TCNGA 2 cut(s) 102, 487
Hpy188III TCNNGA 4 cut(s) 44, 216, 361, 408
Hpy8I GTNNAC 1 cut(s) 290
HpyAV CCTTC 3 cut(s) 122, 248, 398
HpyCH4III ACNGT 5 cut(s) 83, 155, 326, 598, 664
HpyCH4V TGCA 7 cut(s) 12, 62, 321, 338, 509, 578, 660
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 575, 584
HpyF3I CTNAG 7 cut(s) 22, 99, 123, 240, 533, 603, 645
Hsp92II CATG 4 cut(s) 321, 383, 406, 624
Kzo9I GATC 2 cut(s) 274, 490
LmnI GCTCC 1 cut(s) 624
Lsp1109I GCAGC 2 cut(s) 574, 596
LweI GCATC 1 cut(s) 453
MaeI CTAG 4 cut(s) 341, 398, 591, 612
MaeIII GTNAC 3 cut(s) 209, 536, 598
MalI GATC 2 cut(s) 276, 492
MboI GATC 2 cut(s) 274, 490
MboII GAAGA 1 cut(s) 500
MhlI GDGCHC 1 cut(s) 629
MluCI AATT 3 cut(s) 249, 263, 383
MlyI GAGTC 2 cut(s) 91, 206
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 2 cut(s) 493, 574
MroXI GAANNNNTTC 2 cut(s) 258, 387
MseI TTAA 1 cut(s) 266
MslI CAYNNNNRTG 1 cut(s) 691
MspA1I CMGCKG 1 cut(s) 587
MspR9I CCNGG 4 cut(s) 170, 227, 285, 393
Mva1269I GAATGC 1 cut(s) 258
MvaI CCWGG 4 cut(s) 170, 227, 285, 393
MwoI GCNNNNNNNGC 3 cut(s) 18, 575, 584
NdeII GATC 2 cut(s) 274, 490
NlaIII CATG 4 cut(s) 321, 383, 406, 624
NmeAIII GCCGAG 1 cut(s) 595
NmuCI GTSAC 2 cut(s) 209, 598
NspI RCATGY 1 cut(s) 321
PaeI GCATGC 1 cut(s) 321
PaeR7I CTCGAG 1 cut(s) 214
PceI AGGCCT 1 cut(s) 396
PctI GAATGC 1 cut(s) 258
PdmI GAANNNNTTC 2 cut(s) 258, 387
PfeI GAWTC 4 cut(s) 6, 40, 119, 188
PkrI GCNGC 3 cut(s) 454, 586, 589
PleI GAGTC 2 cut(s) 91, 206
PpsI GAGTC 2 cut(s) 91, 206
Psp6I CCWGG 4 cut(s) 168, 225, 283, 391
PspGI CCWGG 4 cut(s) 168, 225, 283, 391
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 1 cut(s) 429
RsaNI GTAC 1 cut(s) 428
RseI CAYNNNNRTG 1 cut(s) 691
SaqAI TTAA 1 cut(s) 266
SatI GCNGC 3 cut(s) 453, 585, 588
Sau3AI GATC 2 cut(s) 274, 490
SchI GAGTC 2 cut(s) 91, 206
ScrFI CCNGG 4 cut(s) 170, 227, 285, 393
SduI GDGCHC 1 cut(s) 629
SetI ASST 4 cut(s) 34, 415, 485, 589
SfaNI GCATC 1 cut(s) 453
Sfr274I CTCGAG 1 cut(s) 214
SlaI CTCGAG 1 cut(s) 214
SmiMI CAYNNNNRTG 1 cut(s) 691
SmlI CTYRAG 1 cut(s) 214
SmoI CTYRAG 1 cut(s) 214
SphI GCATGC 1 cut(s) 321
Sse9I AATT 3 cut(s) 249, 263, 383
SseBI AGGCCT 1 cut(s) 396
SsiI CCGC 1 cut(s) 453
SspMI CTAG 4 cut(s) 341, 398, 591, 612
StuI AGGCCT 1 cut(s) 396
StyD4I CCNGG 4 cut(s) 168, 225, 283, 391
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 5 cut(s) 83, 155, 326, 598, 664
TaqI TCGA 2 cut(s) 215, 328
TasI AATT 3 cut(s) 249, 263, 383
TatI WGTACW 1 cut(s) 427
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 4 cut(s) 6, 40, 119, 188
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TscAI CASTG 4 cut(s) 88, 160, 277, 667
TseFI GTSAC 2 cut(s) 209, 598
TseI GCWGC 2 cut(s) 584, 587
Tsp45I GTSAC 2 cut(s) 209, 598
TspDTI ATGAA 7 cut(s) 69, 180, 312, 396, 487, 558, 675
TspRI CASTG 4 cut(s) 88, 160, 277, 667
XapI RAATTY 1 cut(s) 383
XceI RCATGY 1 cut(s) 321
XhoI CTCGAG 1 cut(s) 214
XmnI GAANNNNTTC 2 cut(s) 258, 387
XspI CTAG 4 cut(s) 341, 398, 591, 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.