Rorug06G0381000

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013254
Physical Location & Seq
Forward (+)
52705053 .. 52706359
1307 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0381000.1

Sequence Viewer

Length: 708 bp
ATGGCGGTTCTGGCAGTGACGATAGGTGATCGCCGCGACGGACTGATGCGCGGCGGCGGGAATGGTAGAGGCTGCTGGCTGGAGGTTGTGGACTTGGGCTGTGCCAAGTGGGCTAGGGTTAACAATAACTACCAACCATGGCCTTTCTCATGGCTCGAAGAGTTTCAGAAAGCTCGGGGTGGAATCAAACAACCGAAAGCGCATCCGCGACCTCGATGGAAACCTGCTGAAAATGGAGCGTTTAAATTGAATGTTGATGCGGCTTTCTTGTCATCAACAACCAAGGGAGGCATTGGCGGAATTTTACGGGATGCATCAGGAAGATTTGTAGCAGCATTTGCCAGTCCAGTGCCATATGCTGCATCTCTGAAACAATGTGAGCTTATGGCAATTAGAGCCGGCATTGATTTCCTTGTTTCCCTTCATATACAGCATGTGGTTGTAGAAAGCGACAGCACCATGGCCATAGCTGAGGCTCTGTGCACAGACCATACTCTCTTGGCCAATGGGGGTTTAATTGATGACATACAGAGGGCCATGCAGACAATTCCAAACGTTCAAATACGCCATCAACCACGCTCCAGCAATATGGTAGCACATAGGCTTGCTGGCATAGGCTTTGAAGCAATTAATAGTTGTATCTGGATAGGTCTAGCACCTACTTGTATTCGTGATGTTCTCCATCACGATTCTCAGTACCTCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

235

Amino Acids

25.64

Weight (kDa)

8.81

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 84 - 203 3.9e-21 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26010
fragaria_vesca FvH4_2g32170
malus_domestica MD08G1073400.v1.1
prunus_persica Prupe.1G414800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310511
rosa_laevigata RLG00000010477
rosa_multiflora Rmu_sc0016392.1_g000006 Rmu_sc0020729.1_g000003 Rmu_sc0035020.1_g000002
rosa_roxburghii Rroxscaffold_7G00158470
rosa_rugosa Rorug06G0380900 Rorug06G0381000 Rorug06G0381100
rosa_samantha Rh6AG495000 Rh6BG504600 Rh6CG509900 Rh6DG495900
rosa_wichuraiana Rw6G043050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 232
AccII CGCG 3 cut(s) 36, 51, 208
AciI CCGC 8 cut(s) 5, 34, 51, 54, 57, 206, 260, 297
AclI AACGTT 1 cut(s) 555
AcoI YGGCCR 2 cut(s) 462, 501
AcsI RAATTY 1 cut(s) 300
AfaI GTAC 1 cut(s) 698
AgsI TTSAA 3 cut(s) 250, 560, 623
AluBI AGCT 3 cut(s) 173, 382, 470
AluI AGCT 3 cut(s) 173, 382, 470
Alw21I GWGCWC 1 cut(s) 485
Alw44I GTGCAC 1 cut(s) 481
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 4 cut(s) 140, 462, 501, 534
ApaLI GTGCAC 1 cut(s) 481
ApeKI GCWGC 3 cut(s) 72, 332, 359
ApoI RAATTY 1 cut(s) 300
AseI ATTAAT 1 cut(s) 630
Asp700I GAANNNNTTC 1 cut(s) 162
AspLEI GCGC 2 cut(s) 51, 202
AspS9I GGNCC 1 cut(s) 534
AsuHPI GGTGA 1 cut(s) 38
AvaI CYCGRG 1 cut(s) 174
BaeGI GKGCMC 1 cut(s) 485
BalI TGGCCA 2 cut(s) 464, 503
Bbv12I GWGCWC 1 cut(s) 485
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 3 cut(s) 59, 344, 346
BccI CCATC 3 cut(s) 210, 576, 690
BfaI CTAG 2 cut(s) 114, 653
BfuAI ACCTGC 1 cut(s) 232
BglI GCCNNNNNGGC 1 cut(s) 110
BisI GCNGC 7 cut(s) 34, 52, 55, 73, 261, 333, 360
BlsI GCNGC 7 cut(s) 35, 53, 56, 74, 262, 334, 361
BmeT110I CYCGRG 1 cut(s) 174
BmgT120I GGNCC 1 cut(s) 534
BmsI GCATC 6 cut(s) 36, 211, 247, 301, 323, 371
BpmI CTGGAG 2 cut(s) 101, 565
Bpu10I CCTNAGC 1 cut(s) 471
BsaJI CCNNGG 3 cut(s) 137, 282, 459
Bse118I RCCGGY 1 cut(s) 398
Bse1I ACTGG 2 cut(s) 342, 347
BseDI CCNNGG 3 cut(s) 137, 282, 459
BseGI GGATG 2 cut(s) 202, 316
BseMII CTCAG 2 cut(s) 462, 707
BseNI ACTGG 2 cut(s) 342, 347
BseSI GKGCMC 1 cut(s) 485
BseXI GCAGC 3 cut(s) 59, 344, 346
Bsh1236I CGCG 3 cut(s) 36, 51, 208
BshFI GGCC 4 cut(s) 142, 464, 503, 536
BsiHKAI GWGCWC 1 cut(s) 485
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 1 cut(s) 399
BsnI GGCC 4 cut(s) 142, 464, 503, 536
BsoBI CYCGRG 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 485
Bsp143I GATC 1 cut(s) 28
Bsp19I CCATGG 2 cut(s) 137, 459
BspACI CCGC 8 cut(s) 5, 34, 51, 54, 57, 206, 260, 297
BspANI GGCC 4 cut(s) 142, 464, 503, 536
BspCNI CTCAG 2 cut(s) 463, 706
BspFNI CGCG 3 cut(s) 36, 51, 208
BspMI ACCTGC 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 398
BsrI ACTGG 2 cut(s) 342, 347
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 3 cut(s) 137, 282, 459
BssMI GATC 1 cut(s) 28
BssT1I CCWWGG 3 cut(s) 137, 282, 459
Bst6I CTCTTC 1 cut(s) 153
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 4 cut(s) 77, 400, 606, 610
BstDEI CTNAG 2 cut(s) 471, 693
BstDSI CCRYGG 2 cut(s) 137, 459
BstF5I GGATG 2 cut(s) 202, 316
BstFNI CGCG 3 cut(s) 36, 51, 208
BstHHI GCGC 2 cut(s) 51, 202
BstKTI GATC 1 cut(s) 31
BstMBI GATC 1 cut(s) 28
BstMWI GCNNNNNNNGC 4 cut(s) 11, 110, 338, 395
BstNSI RCATGY 1 cut(s) 437
BstSLI GKGCMC 1 cut(s) 485
BstUI CGCG 3 cut(s) 36, 51, 208
BstV1I GCAGC 3 cut(s) 59, 344, 346
BstXI CCANNNNNNTGG 1 cut(s) 589
BsuRI GGCC 4 cut(s) 142, 464, 503, 536
BtgI CCRYGG 2 cut(s) 137, 459
BtsCI GGATG 2 cut(s) 202, 316
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 2 cut(s) 21, 354
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 4 cut(s) 77, 400, 606, 610
CfoI GCGC 2 cut(s) 51, 202
Cfr10I RCCGGY 1 cut(s) 398
Cfr13I GGNCC 1 cut(s) 534
Csp6I GTAC 1 cut(s) 697
CviAII CATG 5 cut(s) 138, 150, 434, 460, 538
CviQI GTAC 1 cut(s) 697
DdeI CTNAG 2 cut(s) 471, 693
DpnI GATC 1 cut(s) 30
DpnII GATC 1 cut(s) 28
DraI TTTAAA 1 cut(s) 244
EaeI YGGCCR 2 cut(s) 462, 501
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
EciI GGCGGA 1 cut(s) 312
Eco130I CCWWGG 3 cut(s) 137, 282, 459
Eco88I CYCGRG 1 cut(s) 174
EcoT14I CCWWGG 3 cut(s) 137, 282, 459
EcoT22I ATGCAT 1 cut(s) 316
ErhI CCWWGG 3 cut(s) 137, 282, 459
FaeI CATG 5 cut(s) 141, 153, 437, 463, 541
FatI CATG 5 cut(s) 137, 149, 433, 459, 537
FauI CCCGC 1 cut(s) 50
FauNDI CATATG 1 cut(s) 355
Fnu4HI GCNGC 7 cut(s) 34, 52, 55, 73, 261, 333, 360
FokI GGATG 2 cut(s) 189, 323
Fsp4HI GCNGC 7 cut(s) 34, 52, 55, 73, 261, 333, 360
FspBI CTAG 2 cut(s) 114, 653
GlaI GCGC 2 cut(s) 50, 201
GluI GCNGC 7 cut(s) 34, 52, 55, 73, 261, 333, 360
GsuI CTGGAG 2 cut(s) 101, 565
HaeIII GGCC 4 cut(s) 142, 464, 503, 536
HapII CCGG 1 cut(s) 399
HhaI GCGC 2 cut(s) 51, 202
Hin1II CATG 5 cut(s) 141, 153, 437, 463, 541
Hin6I GCGC 2 cut(s) 49, 200
HinP1I GCGC 2 cut(s) 49, 200
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 2 cut(s) 183, 689
HpaI GTTAAC 1 cut(s) 121
HpaII CCGG 1 cut(s) 399
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 3 cut(s) 91, 121, 483
Hpy188I TCNGA 2 cut(s) 168, 369
Hpy188III TCNNGA 4 cut(s) 318, 643, 671, 686
Hpy8I GTNNAC 3 cut(s) 91, 121, 483
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 1 cut(s) 431
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 4 cut(s) 314, 362, 483, 541
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 110, 338, 395
HpyF3I CTNAG 2 cut(s) 471, 693
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 5 cut(s) 141, 153, 437, 463, 541
HspAI GCGC 2 cut(s) 49, 200
KroI GCCGGC 1 cut(s) 398
KroNI GCCGGC 1 cut(s) 400
KspAI GTTAAC 1 cut(s) 121
Kzo9I GATC 1 cut(s) 28
LmnI GCTCC 2 cut(s) 236, 584
Lsp1109I GCAGC 3 cut(s) 59, 344, 346
LweI GCATC 6 cut(s) 36, 211, 247, 301, 323, 371
MaeI CTAG 2 cut(s) 114, 653
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 1 cut(s) 16
MalI GATC 1 cut(s) 30
MboI GATC 1 cut(s) 28
MboII GAAGA 2 cut(s) 170, 333
MhlI GDGCHC 1 cut(s) 485
MlsI TGGCCA 2 cut(s) 464, 503
MluCI AATT 6 cut(s) 245, 300, 390, 516, 546, 627
MluNI TGGCCA 2 cut(s) 464, 503
MnlI CCTC 6 cut(s) 62, 76, 222, 281, 466, 525
Mox20I TGGCCA 2 cut(s) 464, 503
Mph1103I ATGCAT 1 cut(s) 316
MroNI GCCGGC 1 cut(s) 398
MroXI GAANNNNTTC 1 cut(s) 162
MscI TGGCCA 2 cut(s) 464, 503
MseI TTAA 4 cut(s) 120, 243, 515, 630
Msp20I TGGCCA 2 cut(s) 464, 503
MspI CCGG 1 cut(s) 399
MvnI CGCG 3 cut(s) 36, 51, 208
MwoI GCNNNNNNNGC 4 cut(s) 11, 110, 338, 395
NaeI GCCGGC 1 cut(s) 400
NcoI CCATGG 2 cut(s) 137, 459
NdeI CATATG 1 cut(s) 355
NdeII GATC 1 cut(s) 28
NgoMIV GCCGGC 1 cut(s) 398
NlaIII CATG 5 cut(s) 141, 153, 437, 463, 541
NmuCI GTSAC 1 cut(s) 16
NsiI ATGCAT 1 cut(s) 316
NspI RCATGY 1 cut(s) 437
PdiI GCCGGC 1 cut(s) 400
PdmI GAANNNNTTC 1 cut(s) 162
PfeI GAWTC 2 cut(s) 183, 689
PkrI GCNGC 7 cut(s) 35, 53, 56, 74, 262, 334, 361
PshBI ATTAAT 1 cut(s) 630
Psp1406I AACGTT 1 cut(s) 555
PspPI GGNCC 1 cut(s) 534
RsaI GTAC 1 cut(s) 698
RsaNI GTAC 1 cut(s) 697
SaqAI TTAA 4 cut(s) 120, 243, 515, 630
SatI GCNGC 7 cut(s) 34, 52, 55, 73, 261, 333, 360
Sau3AI GATC 1 cut(s) 28
Sau96I GGNCC 1 cut(s) 534
SduI GDGCHC 1 cut(s) 485
SfaNI GCATC 6 cut(s) 36, 211, 247, 301, 323, 371
Sse9I AATT 6 cut(s) 245, 300, 390, 516, 546, 627
SsiI CCGC 8 cut(s) 5, 34, 51, 54, 57, 206, 260, 297
SspMI CTAG 2 cut(s) 114, 653
StyI CCWWGG 3 cut(s) 137, 282, 459
TaiI ACGT 1 cut(s) 558
TaqI TCGA 2 cut(s) 156, 214
TasI AATT 6 cut(s) 245, 300, 390, 516, 546, 627
TauI GCSGC 4 cut(s) 36, 54, 57, 263
TfiI GAWTC 2 cut(s) 183, 689
Tru1I TTAA 4 cut(s) 120, 243, 515, 630
Tru9I TTAA 4 cut(s) 120, 243, 515, 630
TscAI CASTG 2 cut(s) 21, 354
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 3 cut(s) 72, 332, 359
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 1 cut(s) 413
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 2 cut(s) 21, 354
VneI GTGCAC 1 cut(s) 481
VspI ATTAAT 1 cut(s) 630
XapI RAATTY 1 cut(s) 300
XceI RCATGY 1 cut(s) 437
XmnI GAANNNNTTC 1 cut(s) 162
XspI CTAG 2 cut(s) 114, 653
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.