Rorug06G0381100

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013254
Physical Location & Seq
Forward (+)
52711151 .. 52711639
489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0381100.1

Sequence Viewer

Length: 489 bp
ATGGCGCAGACTAGTATAGGTGCTATGGCTGCTCGTAACCCATATGATTCTGCAAGGGTGACATTGGGAGCGGTAGGGACAATAGCGCTACAGGATAACCATTTGTATTTGGTTGGGCGTCTGCTCGGGCCAAAGGCTCGCTTCCCTGGCTTCAAAGGGACCATTTCCTCGATCTGGCTCATTAAATCGGGGCTTTCTGTCCATGATGCCGGAGATCACTTAATGTTTCAGTTCGACCATGAAGCTGATCGTAATAGGGCATTACATGCAGGACCGTGGTTCTACAGAAACACGATGTTGGTGGTCGGTGATTACGATGGTGTTAGCTCGGTGGAGATAGTTCCGTTGTGGAAGATGGAGGTCTGGGTAGTGGTCAAATGGTTACCAGTGGCCTTTCAAATCAAGGCAACCCTAAATCTGATAGGGTCCGCTGTTGGTCAGGTAACCCGTTTTGATCAACCGGCGCTTCGACAGAAGGAGGCGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

162

Amino Acids

17.91

Weight (kDa)

9.0

Isoelectric Point (pI)

29.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 31 - 104 9.8e-10 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26010
fragaria_vesca FvH4_2g32170
malus_domestica MD08G1073400.v1.1
prunus_persica Prupe.1G414800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310511
rosa_laevigata RLG00000010477
rosa_multiflora Rmu_sc0016392.1_g000006 Rmu_sc0020729.1_g000003 Rmu_sc0035020.1_g000002
rosa_roxburghii Rroxscaffold_7G00158470
rosa_rugosa Rorug06G0380900 Rorug06G0381000 Rorug06G0381100
rosa_samantha Rh6AG495000 Rh6BG504600 Rh6CG509900 Rh6DG495900
rosa_wichuraiana Rw6G043050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 71
AciI CCGC 3 cut(s) 71, 429, 482
AcyI GRCGYC 1 cut(s) 118
AfeI AGCGCT 1 cut(s) 87
AfiI CCNNNNNNNGG 1 cut(s) 174
AgsI TTSAA 2 cut(s) 154, 398
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 2 cut(s) 245, 327
AluI AGCT 2 cut(s) 245, 327
Ama87I CYCGRG 1 cut(s) 125
Aor51HI AGCGCT 1 cut(s) 87
AoxI GGCC 2 cut(s) 128, 390
ApeKI GCWGC 1 cut(s) 29
AspLEI GCGC 3 cut(s) 7, 88, 466
AspS9I GGNCC 4 cut(s) 128, 159, 272, 426
AsuHPI GGTGA 2 cut(s) 70, 320
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 3 cut(s) 159, 272, 426
BbvI GCAGC 1 cut(s) 16
BccI CCATC 2 cut(s) 311, 349
BciT130I CCWGG 1 cut(s) 147
BclI TGATCA 1 cut(s) 454
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 2 cut(s) 89, 283
BfoI RGCGCY 2 cut(s) 89, 467
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 1 cut(s) 147
Bme18I GGWCC 3 cut(s) 159, 272, 426
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 4 cut(s) 128, 159, 272, 426
BmiI GGNNCC 2 cut(s) 160, 427
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 1 cut(s) 196
BsaHI GRCGYC 1 cut(s) 118
BsaJI CCNNGG 2 cut(s) 145, 275
Bsc4I CCNNNNNNNGG 1 cut(s) 174
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 386
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 2 cut(s) 145, 275
BseLI CCNNNNNNNGG 1 cut(s) 174
BseNI ACTGG 1 cut(s) 386
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 2 cut(s) 130, 392
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 2 cut(s) 210, 461
BslFI GGGAC 2 cut(s) 91, 172
BslI CCNNNNNNNGG 1 cut(s) 174
BsmFI GGGAC 2 cut(s) 91, 172
BsnI GGCC 2 cut(s) 130, 392
BsoBI CYCGRG 1 cut(s) 125
Bsp143I GATC 4 cut(s) 171, 214, 247, 454
BspACI CCGC 3 cut(s) 71, 429, 482
BspANI GGCC 2 cut(s) 130, 392
BspLI GGNNCC 2 cut(s) 160, 427
BsrBI CCGCTC 1 cut(s) 71
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 1 cut(s) 386
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 2 cut(s) 145, 275
BssMI GATC 4 cut(s) 171, 214, 247, 454
BssNI GRCGYC 1 cut(s) 118
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 118
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 139
BstDSI CCRYGG 1 cut(s) 275
BstEII GGTNACC 2 cut(s) 381, 442
BstH2I RGCGCY 2 cut(s) 89, 467
BstHHI GCGC 3 cut(s) 7, 88, 466
BstKTI GATC 4 cut(s) 174, 217, 250, 457
BstMBI GATC 4 cut(s) 171, 214, 247, 454
BstMWI GCNNNNNNNGC 3 cut(s) 29, 147, 266
BstNI CCWGG 1 cut(s) 147
BstNSI RCATGY 1 cut(s) 269
BstPI GGTNACC 2 cut(s) 381, 442
BstSCI CCNGG 1 cut(s) 145
BstSFI CTRYAG 2 cut(s) 89, 283
BstV1I GCAGC 1 cut(s) 16
BsuRI GGCC 2 cut(s) 130, 392
BtgI CCRYGG 1 cut(s) 275
BtsIMutI CAGTG 1 cut(s) 393
Cac8I GCNNGC 1 cut(s) 139
CfoI GCGC 3 cut(s) 7, 88, 466
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 4 cut(s) 128, 159, 272, 426
CseI GACGC 1 cut(s) 107
CviAII CATG 3 cut(s) 203, 239, 266
CviJI RGCY 9 cut(s) 29, 130, 137, 150, 178, 193, 245, 327, 392
CviKI_1 RGCY 9 cut(s) 29, 130, 137, 150, 178, 193, 245, 327, 392
DpnI GATC 4 cut(s) 173, 216, 249, 456
DpnII GATC 4 cut(s) 171, 214, 247, 454
Eco47I GGWCC 3 cut(s) 159, 272, 426
Eco47III AGCGCT 1 cut(s) 87
Eco88I CYCGRG 1 cut(s) 125
Eco91I GGTNACC 2 cut(s) 381, 442
EcoO65I GGTNACC 2 cut(s) 381, 442
EcoRII CCWGG 1 cut(s) 145
FaeI CATG 3 cut(s) 206, 242, 269
FaiI YATR 7 cut(s) 17, 26, 43, 45, 204, 240, 267
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FaqI GGGAC 2 cut(s) 91, 172
FatI CATG 3 cut(s) 202, 238, 265
FauNDI CATATG 1 cut(s) 43
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 1 cut(s) 30
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 1 cut(s) 12
GlaI GCGC 3 cut(s) 6, 87, 465
GluI GCNGC 1 cut(s) 30
HaeII RGCGCY 2 cut(s) 89, 467
HaeIII GGCC 2 cut(s) 130, 392
HapII CCGG 2 cut(s) 210, 461
HgaI GACGC 1 cut(s) 107
HhaI GCGC 3 cut(s) 7, 88, 466
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 3 cut(s) 206, 242, 269
Hin6I GCGC 3 cut(s) 5, 86, 464
HinP1I GCGC 3 cut(s) 5, 86, 464
HinfI GANTC 1 cut(s) 47
HpaII CCGG 2 cut(s) 210, 461
HphI GGTGA 2 cut(s) 70, 320
Hpy188I TCNGA 1 cut(s) 420
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4V TGCA 2 cut(s) 53, 269
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 147, 266
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 3 cut(s) 206, 242, 269
HspAI GCGC 3 cut(s) 5, 86, 464
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 4 cut(s) 171, 214, 247, 454
LmnI GCTCC 1 cut(s) 68
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 1 cut(s) 196
MaeI CTAG 1 cut(s) 12
MaeIII GTNAC 4 cut(s) 35, 58, 381, 442
MalI GATC 4 cut(s) 173, 216, 249, 456
MbiI CCGCTC 1 cut(s) 71
MboI GATC 4 cut(s) 171, 214, 247, 454
MboII GAAGA 1 cut(s) 364
MnlI CCTC 3 cut(s) 178, 352, 472
MseI TTAA 2 cut(s) 183, 221
MspA1I CMGCKG 1 cut(s) 431
MspI CCGG 2 cut(s) 210, 461
MspR9I CCNGG 1 cut(s) 147
MvaI CCWGG 1 cut(s) 147
MwoI GCNNNNNNNGC 3 cut(s) 29, 147, 266
NdeI CATATG 1 cut(s) 43
NdeII GATC 4 cut(s) 171, 214, 247, 454
NlaIII CATG 3 cut(s) 206, 242, 269
NlaIV GGNNCC 2 cut(s) 160, 427
NmuCI GTSAC 1 cut(s) 58
NspI RCATGY 1 cut(s) 269
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PfeI GAWTC 1 cut(s) 47
PkrI GCNGC 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 145
PspEI GGTNACC 2 cut(s) 381, 442
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 2 cut(s) 160, 427
PspPI GGNCC 4 cut(s) 128, 159, 272, 426
SaqAI TTAA 2 cut(s) 183, 221
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 4 cut(s) 171, 214, 247, 454
Sau96I GGNCC 4 cut(s) 128, 159, 272, 426
ScrFI CCNGG 1 cut(s) 147
SetI ASST 5 cut(s) 22, 247, 329, 363, 444
SfaNI GCATC 1 cut(s) 196
SfcI CTRYAG 2 cut(s) 89, 283
SinI GGWCC 3 cut(s) 159, 272, 426
SpeI ACTAGT 1 cut(s) 11
SsiI CCGC 3 cut(s) 71, 429, 482
SspMI CTAG 1 cut(s) 12
StyD4I CCNGG 1 cut(s) 145
TaaI ACNGT 1 cut(s) 276
TaqI TCGA 3 cut(s) 170, 234, 469
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 2 cut(s) 183, 221
Tru9I TTAA 2 cut(s) 183, 221
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 1 cut(s) 255
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 1 cut(s) 393
VpaK11BI GGWCC 3 cut(s) 159, 272, 426
XceI RCATGY 1 cut(s) 269
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.