Rh6DG495900

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013254
Physical Location & Seq
Reverse (-)
65382984 .. 65386590
3607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG495900.1

Sequence Viewer

Length: 1014 bp
ATGGGAATCTGCATATCCTGCTCAGTTTCAGGTCAAAAGATTCAAGATGGCAATGAAAATGCAATGTTCTTTGGAAAGAACAGTGGTTCTTTTGGGACTCAGAGCCTTGGTTCGCTTTATTCTAAGGAAGGAAGCAAAGGACTAAACCAAGACAGTGCCATTCTTTACCAGGGCTATGGAGTTGGAGATTCATCTTTCTGTGGAGTATTTGATGGGCATGGAAAGAATGGTCACATAGTGAGTGAGATGGTGAAGAATCACCTACCAGCACTACTACAGAGCCAAATGGAAGCTCTTGAACAAGTCACTTTAGTAACACAATCAGATGGTGAACAAAATCTTAACGACGAAACGGATACTGAATCAGAACCAAGCAAGAACTTTGATAAGTGGGAAGAGGCACTGGTTAATGCCTTCAAAGTAATGGACAAGGAGATTAAGCTCCAAGAAAATTTGGACTGTTCTTGTAGTGGAAGCACTGCTGTTGTTGTCATAAGACAGGGCGAGGACCTTTTTATAGCTAACTTGGGTGACTCGAGAGCCATCCTGGGAACAATGACTGAGAAAGGAATTGAAGGCATTCAATTAACCACTGATCTAAAGCCTGGTTTACCAAATGAAGCAGAACGAATAAGAGCATGCAATGGTCGAGTGATTGCACTAGAACACGAAAAACACATCCAGAGAGTGTGGTTGCCCCATGAATTCCTTCCAGGCCTAGCCATGTCGCGAGCTTTTGGGGACTTTGTACTCAAAGATTATGGCATCATTGCCGTCCCTGATGTCTCCCATCATCGCCTGACCTCCGAAAATCAGTTCATTGTTCTTGCAACTGATGGGGTATGGGATGTGCTTAGTAACAATGAAGTTGCGTCTATTGTGTGGGAAGCCGAGAGTGCAGAGGCAGCTGCTAGGACTGTGACTGAGACGGCTAGAGCCACATGGAGCACAAAGTACCCAACTTCTAAGATAGACGATTGCACTGTGGTTTGCCTGTTTTTCAGTTCATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

337

Amino Acids

36.76

Weight (kDa)

4.75

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 64 - 313 1.1e-43 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26010
fragaria_vesca FvH4_2g32170
malus_domestica MD08G1073400.v1.1
prunus_persica Prupe.1G414800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310511
rosa_laevigata RLG00000010477
rosa_multiflora Rmu_sc0016392.1_g000006 Rmu_sc0020729.1_g000003 Rmu_sc0035020.1_g000002
rosa_roxburghii Rroxscaffold_7G00158470
rosa_rugosa Rorug06G0380900 Rorug06G0381000 Rorug06G0381100
rosa_samantha Rh6AG495000 Rh6BG504600 Rh6CG509900 Rh6DG495900
rosa_wichuraiana Rw6G043050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 730
AcsI RAATTY 2 cut(s) 451, 704
AdeI CACNNNGTG 1 cut(s) 238
AfaI GTAC 2 cut(s) 750, 956
AgsI TTSAA 5 cut(s) 44, 299, 418, 575, 584
AjnI CCWGG 4 cut(s) 168, 546, 604, 712
AluBI AGCT 5 cut(s) 293, 442, 521, 734, 908
AluI AGCT 5 cut(s) 293, 442, 521, 734, 908
Alw21I GWGCWC 1 cut(s) 950
Alw26I GTCTC 2 cut(s) 790, 920
Ama87I CYCGRG 1 cut(s) 535
AoxI GGCC 1 cut(s) 715
ApeKI GCWGC 2 cut(s) 905, 908
ApoI RAATTY 2 cut(s) 451, 704
Asp700I GAANNNNTTC 2 cut(s) 579, 708
AspS9I GGNCC 1 cut(s) 508
AsuHPI GGTGA 4 cut(s) 251, 262, 341, 542
AvaI CYCGRG 1 cut(s) 535
AvaII GGWCC 1 cut(s) 508
Bbv12I GWGCWC 1 cut(s) 950
BbvI GCAGC 2 cut(s) 895, 917
BccI CCATC 7 cut(s) 41, 206, 241, 320, 551, 798, 830
BceAI ACGGC 2 cut(s) 758, 945
BciT130I CCWGG 4 cut(s) 170, 548, 606, 714
BciVI GTATCC 1 cut(s) 349
BcoDI GTCTC 2 cut(s) 790, 920
BfaI CTAG 5 cut(s) 662, 719, 912, 933, 1012
BfmI CTRYAG 1 cut(s) 275
BfuI GTATCC 1 cut(s) 349
BisI GCNGC 2 cut(s) 906, 909
BlsI GCNGC 2 cut(s) 907, 910
Bme1390I CCNGG 4 cut(s) 170, 548, 606, 714
Bme18I GGWCC 1 cut(s) 508
BmeT110I CYCGRG 1 cut(s) 535
BmgT120I GGNCC 1 cut(s) 508
BmrFI CCNGG 4 cut(s) 170, 548, 606, 714
BmsI GCATC 1 cut(s) 774
BsaJI CCNNGG 3 cut(s) 106, 169, 547
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 4 cut(s) 58, 69, 649, 768
BseBI CCWGG 4 cut(s) 170, 548, 606, 714
BseDI CCNNGG 3 cut(s) 106, 169, 547
BseGI GGATG 3 cut(s) 543, 678, 853
BseMI GCAATG 4 cut(s) 58, 69, 649, 768
BseMII CTCAG 4 cut(s) 36, 113, 552, 915
BseNI ACTGG 1 cut(s) 408
BseXI GCAGC 2 cut(s) 895, 917
BsgI GTGCAG 1 cut(s) 918
Bsh1236I CGCG 1 cut(s) 730
BshFI GGCC 1 cut(s) 717
BsiHKAI GWGCWC 1 cut(s) 950
BsiHKCI CYCGRG 1 cut(s) 535
BslFI GGGAC 3 cut(s) 109, 755, 761
BsmAI GTCTC 2 cut(s) 790, 920
BsmBI CGTCTC 1 cut(s) 920
BsmFI GGGAC 3 cut(s) 109, 755, 761
BsmI GAATGC 1 cut(s) 579
BsnI GGCC 1 cut(s) 717
BsoBI CYCGRG 1 cut(s) 535
Bsp1286I GDGCHC 1 cut(s) 950
Bsp143I GATC 1 cut(s) 595
Bsp68I TCGCGA 1 cut(s) 730
BspANI GGCC 1 cut(s) 717
BspCNI CTCAG 4 cut(s) 35, 112, 553, 916
BspFNI CGCG 1 cut(s) 730
BsrDI GCAATG 4 cut(s) 58, 69, 649, 768
BsrI ACTGG 1 cut(s) 408
BssECI CCNNGG 3 cut(s) 106, 169, 547
BssMI GATC 1 cut(s) 595
BssT1I CCWWGG 1 cut(s) 106
Bst2UI CCWGG 4 cut(s) 170, 548, 606, 714
Bst4CI ACNGT 5 cut(s) 83, 155, 461, 919, 985
Bst6I CTCTTC 1 cut(s) 390
BstAPI GCANNNNNTGC 1 cut(s) 18
BstC8I GCNNGC 2 cut(s) 640, 732
BstDEI CTNAG 7 cut(s) 22, 99, 123, 561, 854, 924, 966
BstF5I GGATG 3 cut(s) 543, 678, 853
BstFNI CGCG 1 cut(s) 730
BstKTI GATC 1 cut(s) 598
BstMAI GTCTC 2 cut(s) 790, 920
BstMBI GATC 1 cut(s) 595
BstMWI GCNNNNNNNGC 3 cut(s) 18, 896, 905
BstNI CCWGG 4 cut(s) 170, 548, 606, 714
BstNSI RCATGY 1 cut(s) 642
BstSCI CCNGG 4 cut(s) 168, 546, 604, 712
BstSFI CTRYAG 1 cut(s) 275
BstUI CGCG 1 cut(s) 730
BstV1I GCAGC 2 cut(s) 895, 917
BstXI CCANNNNNNTGG 1 cut(s) 176
BsuI GTATCC 1 cut(s) 349
BsuRI GGCC 1 cut(s) 717
BtgZI GCGATG 1 cut(s) 779
BtsCI GGATG 3 cut(s) 543, 678, 853
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 6 cut(s) 88, 160, 401, 477, 591, 981
BtuMI TCGCGA 1 cut(s) 730
Cac8I GCNNGC 2 cut(s) 640, 732
Cfr13I GGNCC 1 cut(s) 508
CseI GACGC 1 cut(s) 861
Csp6I GTAC 2 cut(s) 749, 955
CviAII CATG 5 cut(s) 218, 639, 701, 724, 942
CviQI GTAC 2 cut(s) 749, 955
DdeI CTNAG 7 cut(s) 22, 99, 123, 561, 854, 924, 966
DpnI GATC 1 cut(s) 597
DpnII GATC 1 cut(s) 595
DraIII CACNNNGTG 1 cut(s) 238
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 106
Eco147I AGGCCT 1 cut(s) 717
Eco47I GGWCC 1 cut(s) 508
Eco88I CYCGRG 1 cut(s) 535
EcoO109I RGGNCCY 1 cut(s) 508
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 4 cut(s) 168, 546, 604, 712
EcoT14I CCWWGG 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 106
Esp3I CGTCTC 1 cut(s) 920
FaeI CATG 5 cut(s) 221, 642, 704, 727, 945
FaqI GGGAC 3 cut(s) 109, 755, 761
FatI CATG 5 cut(s) 217, 638, 700, 723, 941
Fnu4HI GCNGC 2 cut(s) 906, 909
FokI GGATG 3 cut(s) 530, 665, 860
Fsp4HI GCNGC 2 cut(s) 906, 909
FspBI CTAG 5 cut(s) 662, 719, 912, 933, 1012
GluI GCNGC 2 cut(s) 906, 909
HaeIII GGCC 1 cut(s) 717
HgaI GACGC 1 cut(s) 861
Hin1II CATG 5 cut(s) 221, 642, 704, 727, 945
HinfI GANTC 7 cut(s) 6, 40, 97, 188, 256, 362, 533
HphI GGTGA 4 cut(s) 251, 262, 341, 542
Hpy166II GTNNAC 2 cut(s) 332, 611
Hpy188I TCNGA 4 cut(s) 102, 325, 367, 808
Hpy188III TCNNGA 5 cut(s) 44, 296, 537, 682, 729
Hpy8I GTNNAC 2 cut(s) 332, 611
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 4 cut(s) 122, 424, 569, 719
HpyCH4III ACNGT 5 cut(s) 83, 155, 461, 919, 985
HpyCH4V TGCA 7 cut(s) 12, 62, 642, 659, 830, 899, 981
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 896, 905
HpyF3I CTNAG 7 cut(s) 22, 99, 123, 561, 854, 924, 966
Hsp92II CATG 5 cut(s) 221, 642, 704, 727, 945
Kzo9I GATC 1 cut(s) 595
LmnI GCTCC 2 cut(s) 447, 945
Lsp1109I GCAGC 2 cut(s) 895, 917
LweI GCATC 1 cut(s) 774
MaeI CTAG 5 cut(s) 662, 719, 912, 933, 1012
MaeIII GTNAC 6 cut(s) 230, 304, 313, 530, 857, 919
MalI GATC 1 cut(s) 597
MboI GATC 1 cut(s) 595
MboII GAAGA 2 cut(s) 265, 407
MhlI GDGCHC 1 cut(s) 950
MluCI AATT 4 cut(s) 451, 570, 584, 704
MlyI GAGTC 2 cut(s) 91, 527
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 4 cut(s) 391, 499, 814, 895
MroXI GAANNNNTTC 2 cut(s) 579, 708
MseI TTAA 4 cut(s) 342, 408, 438, 587
MspA1I CMGCKG 1 cut(s) 908
MspR9I CCNGG 4 cut(s) 170, 548, 606, 714
Mva1269I GAATGC 1 cut(s) 579
MvaI CCWGG 4 cut(s) 170, 548, 606, 714
MvnI CGCG 1 cut(s) 730
MwoI GCNNNNNNNGC 3 cut(s) 18, 896, 905
NdeII GATC 1 cut(s) 595
NlaIII CATG 5 cut(s) 221, 642, 704, 727, 945
NmeAIII GCCGAG 1 cut(s) 916
NmuCI GTSAC 4 cut(s) 230, 304, 530, 919
NruI TCGCGA 1 cut(s) 730
NspI RCATGY 1 cut(s) 642
PaeI GCATGC 1 cut(s) 642
PaeR7I CTCGAG 1 cut(s) 535
PceI AGGCCT 1 cut(s) 717
PctI GAATGC 1 cut(s) 579
PdmI GAANNNNTTC 2 cut(s) 579, 708
PfeI GAWTC 5 cut(s) 6, 40, 188, 256, 362
PkrI GCNGC 2 cut(s) 907, 910
PleI GAGTC 2 cut(s) 91, 527
PpsI GAGTC 2 cut(s) 91, 527
PpuMI RGGWCCY 1 cut(s) 508
Psp5II RGGWCCY 1 cut(s) 508
Psp6I CCWGG 4 cut(s) 168, 546, 604, 712
PspGI CCWGG 4 cut(s) 168, 546, 604, 712
PspPI GGNCC 1 cut(s) 508
PspPPI RGGWCCY 1 cut(s) 508
PvuII CAGCTG 1 cut(s) 908
RruI TCGCGA 1 cut(s) 730
RsaI GTAC 2 cut(s) 750, 956
RsaNI GTAC 2 cut(s) 749, 955
SaqAI TTAA 4 cut(s) 342, 408, 438, 587
SatI GCNGC 2 cut(s) 906, 909
Sau3AI GATC 1 cut(s) 595
Sau96I GGNCC 1 cut(s) 508
SchI GAGTC 2 cut(s) 91, 527
ScrFI CCNGG 4 cut(s) 170, 548, 606, 714
SduI GDGCHC 1 cut(s) 950
SetI ASST 9 cut(s) 34, 264, 295, 444, 513, 523, 736, 806, 910
SfaNI GCATC 1 cut(s) 774
SfcI CTRYAG 1 cut(s) 275
Sfr274I CTCGAG 1 cut(s) 535
SinI GGWCC 1 cut(s) 508
SlaI CTCGAG 1 cut(s) 535
SmlI CTYRAG 1 cut(s) 535
SmoI CTYRAG 1 cut(s) 535
SphI GCATGC 1 cut(s) 642
Sse9I AATT 4 cut(s) 451, 570, 584, 704
SseBI AGGCCT 1 cut(s) 717
SspMI CTAG 5 cut(s) 662, 719, 912, 933, 1012
StuI AGGCCT 1 cut(s) 717
StyD4I CCNGG 4 cut(s) 168, 546, 604, 712
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 5 cut(s) 83, 155, 461, 919, 985
TaqI TCGA 2 cut(s) 536, 649
TasI AATT 4 cut(s) 451, 570, 584, 704
TatI WGTACW 1 cut(s) 748
TfiI GAWTC 5 cut(s) 6, 40, 188, 256, 362
Tru1I TTAA 4 cut(s) 342, 408, 438, 587
Tru9I TTAA 4 cut(s) 342, 408, 438, 587
TscAI CASTG 6 cut(s) 88, 160, 408, 484, 598, 988
TseFI GTSAC 4 cut(s) 230, 304, 530, 919
TseI GCWGC 2 cut(s) 905, 908
Tsp45I GTSAC 4 cut(s) 230, 304, 530, 919
TspDTI ATGAA 7 cut(s) 69, 180, 633, 717, 808, 879, 996
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 6 cut(s) 88, 160, 408, 484, 598, 988
VpaK11BI GGWCC 1 cut(s) 508
XapI RAATTY 2 cut(s) 451, 704
XceI RCATGY 1 cut(s) 642
XhoI CTCGAG 1 cut(s) 535
XmnI GAANNNNTTC 2 cut(s) 579, 708
XspI CTAG 5 cut(s) 662, 719, 912, 933, 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.