MD08G1073400.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013254
Physical Location & Seq
Forward (+)
5926132 .. 5927895
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1073400.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGGACTCTGCATATCTTGTGCTTCTTCATCTCACAAAATTCAGGACGGCAATCGAACTTCAATGCCATACGAAGAGAACAATGATTCTAGTGGGATTCCTATGCTTGGTTCCCTTCATTCCATGGAAGGAAGCAAAGGAGTAAACCAAGATAGTGCCATGCTTTGCCAGGGCTATGGGAAGGAAGGTGAAGCTCTGTGTGGAGTTTTCGACGGGCATGGGAAATTCGGGCACCGGGTGAGTCAGTTGGTGAGAAGCAACCTTCCATCTCTTTTACTGAACAAAATGAAAGCTCTTGATCATCAACGAGTCACTGCAGAAGCCGAGGTTGATGGTTCCCAAAACCCCGTCAGTTGCAAGGACAACGAACCAGAAGATTCAGAAACAAGCAAGAAGTTTCAGAAATGGAAAGAAGCATTTGTTAGCTCCTTCAGAGTTATGGACAAGGAGATTAAGCTTCAACAGAATTTGGACAACTCTTGCAGTGGAACCACTGCTGTTGTAGTTGTAAAACAGAGTGAGGATCTTTTTATAGCAAATCTGGGAGATTCAAGGGCAGTTCTAGGAACAATTACAGAGAACGGAATTAAGGCCATTCAATTGACCACTGATCTCAAGCCCGGTGTACCTAGTGAAGCAGAACGAATAAGGGCGTGTGGCGGTCGAGTGCTTGCACTAAAGCAAGAACCTAACATCCAGAGAGTGTGGCTGCCTCACGTTGACCTCCCGGGACTAGCCATGTCCCGAGCTTTCGGAGACTTTATACTCAAAGACCACGGCATCATTGCCACCCCAGATGTCTCCCACCACCGTCTCACTTCCAACGATCAGTTCGTTGTCCTTGCAACCGACGGGGTTTGGGACGTGCTCAGCAACAGTGAAGTTGCGTCCATTGTGTGGGAGGCGGAGAGCGCACACGCAGCAGCGAGGGCGGTGACTGAGGCAGCTTCGGCGATGTGGAGGAAGAAGTTCCCTAACGCTAAGAGGGATGACTGCACTGTTGTTTGTTTGTTTTTGCAAGAAAATATTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

345

Amino Acids

37.44

Weight (kDa)

5.72

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 59 - 314 3.2e-42 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26010
fragaria_vesca FvH4_2g32170
malus_domestica MD08G1073400.v1.1
prunus_persica Prupe.1G414800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0310511
rosa_laevigata RLG00000010477
rosa_multiflora Rmu_sc0016392.1_g000006 Rmu_sc0020729.1_g000003 Rmu_sc0035020.1_g000002
rosa_roxburghii Rroxscaffold_7G00158470
rosa_rugosa Rorug06G0380900 Rorug06G0381000 Rorug06G0381100
rosa_samantha Rh6AG495000 Rh6BG504600 Rh6CG509900 Rh6DG495900
rosa_wichuraiana Rw6G043050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 231
AccB7I CCANNNNNTGG 1 cut(s) 897
AciI CCGC 3 cut(s) 660, 905, 932
AclWI GGATC 1 cut(s) 531
AcsI RAATTY 3 cut(s) 39, 224, 466
AcuI CTGAAG 1 cut(s) 415
AdeI CACNNNGTG 1 cut(s) 238
AfaI GTAC 1 cut(s) 627
AfiI CCNNNNNNNGG 2 cut(s) 107, 897
AgsI TTSAA 4 cut(s) 63, 461, 552, 599
AjiI CACGTC 1 cut(s) 865
AjnI CCWGG 1 cut(s) 168
AjuI GAANNNNNNNTTGG 2 cut(s) 815, 847
AloI GAACNNNNNNTCC 2 cut(s) 678, 710
AluBI AGCT 6 cut(s) 194, 293, 426, 457, 749, 947
AluI AGCT 6 cut(s) 194, 293, 426, 457, 749, 947
Alw21I GWGCWC 1 cut(s) 870
Alw26I GTCTC 3 cut(s) 750, 805, 818
AlwI GGATC 1 cut(s) 531
Ama87I CYCGRG 2 cut(s) 727, 744
AoxI GGCC 1 cut(s) 591
ApeKI GCWGC 4 cut(s) 709, 920, 923, 944
ApoI RAATTY 3 cut(s) 39, 224, 466
Asp700I GAANNNNTTC 1 cut(s) 968
AspLEI GCGC 1 cut(s) 914
AsuC2I CCSGG 4 cut(s) 236, 621, 728, 729
AsuHPI GGTGA 4 cut(s) 200, 250, 262, 946
AvaI CYCGRG 2 cut(s) 727, 744
BaeGI GKGCMC 1 cut(s) 234
BanI GGYRCC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 870
BbvI GCAGC 4 cut(s) 696, 932, 935, 956
BccI CCATC 2 cut(s) 274, 326
BceAI ACGGC 2 cut(s) 64, 793
BciT130I CCWGG 1 cut(s) 170
BclI TGATCA 1 cut(s) 298
BcnI CCSGG 4 cut(s) 236, 621, 728, 729
BcoDI GTCTC 3 cut(s) 750, 805, 818
BfaI CTAG 4 cut(s) 90, 563, 630, 734
BfmI CTRYAG 1 cut(s) 315
BisI GCNGC 4 cut(s) 710, 921, 924, 945
BlpI GCTNAGC 1 cut(s) 869
BlsI GCNGC 4 cut(s) 711, 922, 925, 946
Bme1390I CCNGG 5 cut(s) 170, 236, 621, 728, 729
BmeT110I CYCGRG 2 cut(s) 727, 744
BmgBI CACGTC 1 cut(s) 865
BmiI GGNNCC 4 cut(s) 112, 233, 337, 490
BmrFI CCNGG 5 cut(s) 170, 236, 621, 728, 729
BmsI GCATC 1 cut(s) 789
Bpu1102I GCTNAGC 1 cut(s) 869
BpuEI CTTGAG 1 cut(s) 599
BpuMI CCSGG 4 cut(s) 236, 621, 728, 729
BsaJI CCNNGG 5 cut(s) 123, 169, 324, 727, 775
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 897
Bse3DI GCAATG 1 cut(s) 783
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 5 cut(s) 123, 169, 324, 727, 775
BseGI GGATG 2 cut(s) 693, 994
BseLI CCNNNNNNNGG 2 cut(s) 107, 897
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 2 cut(s) 883, 930
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 4 cut(s) 696, 932, 935, 956
BsgI GTGCAG 1 cut(s) 979
Bsh1285I CGRYCG 1 cut(s) 664
BshFI GGCC 1 cut(s) 593
BshNI GGYRCC 1 cut(s) 231
BsiEI CGRYCG 1 cut(s) 664
BsiHKAI GWGCWC 1 cut(s) 870
BsiHKCI CYCGRG 2 cut(s) 727, 744
BsiSI CCGG 3 cut(s) 235, 621, 728
BslFI GGGAC 4 cut(s) 18, 727, 744, 875
BslI CCNNNNNNNGG 2 cut(s) 107, 897
BsmAI GTCTC 3 cut(s) 750, 805, 818
BsmBI CGTCTC 1 cut(s) 818
BsmFI GGGAC 4 cut(s) 18, 727, 744, 875
BsnI GGCC 1 cut(s) 593
BsoBI CYCGRG 2 cut(s) 727, 744
Bsp1286I GDGCHC 2 cut(s) 234, 870
Bsp143I GATC 4 cut(s) 298, 523, 610, 826
Bsp1720I GCTNAGC 1 cut(s) 869
Bsp19I CCATGG 1 cut(s) 123
BspACI CCGC 3 cut(s) 660, 905, 932
BspANI GGCC 1 cut(s) 593
BspCNI CTCAG 2 cut(s) 882, 931
BspLI GGNNCC 4 cut(s) 112, 233, 337, 490
BspMAI CTGCAG 1 cut(s) 319
BspPI GGATC 1 cut(s) 531
BspT107I GGYRCC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 783
BssECI CCNNGG 5 cut(s) 123, 169, 324, 727, 775
BssMI GATC 4 cut(s) 298, 523, 610, 826
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 3 cut(s) 812, 878, 1000
Bst6I CTCTTC 1 cut(s) 69
BstC8I GCNNGC 1 cut(s) 672
BstDEI CTNAG 3 cut(s) 869, 939, 981
BstDSI CCRYGG 2 cut(s) 123, 775
BstF5I GGATG 2 cut(s) 693, 994
BstHHI GCGC 1 cut(s) 914
BstKTI GATC 4 cut(s) 301, 526, 613, 829
BstMAI GTCTC 3 cut(s) 750, 805, 818
BstMBI GATC 4 cut(s) 298, 523, 610, 826
BstMCI CGRYCG 1 cut(s) 664
BstMWI GCNNNNNNNGC 4 cut(s) 911, 920, 929, 950
BstNI CCWGG 1 cut(s) 170
BstSCI CCNGG 5 cut(s) 168, 234, 619, 726, 727
BstSFI CTRYAG 1 cut(s) 315
BstSLI GKGCMC 1 cut(s) 234
BstV1I GCAGC 4 cut(s) 696, 932, 935, 956
BstX2I RGATCY 1 cut(s) 523
BstXI CCANNNNNNTGG 1 cut(s) 176
BstYI RGATCY 1 cut(s) 523
BsuRI GGCC 1 cut(s) 593
BtgI CCRYGG 2 cut(s) 123, 775
BtgZI GCGATG 1 cut(s) 968
BtrI CACGTC 1 cut(s) 865
BtsCI GGATG 2 cut(s) 693, 994
BtsI GCAGTG 3 cut(s) 312, 490, 492
BtsIMutI CAGTG 6 cut(s) 312, 490, 492, 606, 883, 996
Cac8I GCNNGC 1 cut(s) 672
CfoI GCGC 1 cut(s) 914
Cfr9I CCCGGG 1 cut(s) 727
CseI GACGC 1 cut(s) 876
Csp6I GTAC 1 cut(s) 626
CspCI CAANNNNNGTGG 2 cut(s) 481, 516
CviAII CATG 4 cut(s) 124, 160, 218, 739
CviQI GTAC 1 cut(s) 626
DdeI CTNAG 3 cut(s) 869, 939, 981
DpnI GATC 4 cut(s) 300, 525, 612, 828
DpnII GATC 4 cut(s) 298, 523, 610, 826
DraIII CACNNNGTG 1 cut(s) 238
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
EciI GGCGGA 1 cut(s) 920
Eco130I CCWWGG 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 415
Eco88I CYCGRG 2 cut(s) 727, 744
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 123
Esp3I CGTCTC 1 cut(s) 818
FaeI CATG 4 cut(s) 127, 163, 221, 742
FaqI GGGAC 4 cut(s) 18, 727, 744, 875
FatI CATG 4 cut(s) 123, 159, 217, 738
FbaI TGATCA 1 cut(s) 298
Fnu4HI GCNGC 4 cut(s) 710, 921, 924, 945
FokI GGATG 2 cut(s) 680, 1001
Fsp4HI GCNGC 4 cut(s) 710, 921, 924, 945
FspBI CTAG 4 cut(s) 90, 563, 630, 734
GlaI GCGC 1 cut(s) 913
GluI GCNGC 4 cut(s) 710, 921, 924, 945
HaeIII GGCC 1 cut(s) 593
HapII CCGG 3 cut(s) 235, 621, 728
HgaI GACGC 1 cut(s) 876
HhaI GCGC 1 cut(s) 914
Hin1II CATG 4 cut(s) 127, 163, 221, 742
Hin6I GCGC 1 cut(s) 912
HinP1I GCGC 1 cut(s) 912
HincII GTYRAC 1 cut(s) 721
HindII GTYRAC 1 cut(s) 721
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 7 cut(s) 6, 86, 97, 241, 309, 377, 548
HpaII CCGG 3 cut(s) 235, 621, 728
HphI GGTGA 4 cut(s) 200, 250, 262, 946
Hpy166II GTNNAC 3 cut(s) 145, 626, 721
Hpy188I TCNGA 4 cut(s) 382, 402, 434, 755
Hpy188III TCNNGA 4 cut(s) 44, 296, 697, 744
Hpy8I GTNNAC 3 cut(s) 145, 626, 721
Hpy99I CGWCG 2 cut(s) 215, 854
HpyAV CCTTC 6 cut(s) 122, 125, 175, 179, 272, 439
HpyCH4III ACNGT 3 cut(s) 812, 878, 1000
HpyCH4IV ACGT 2 cut(s) 717, 864
HpyCH4V TGCA 8 cut(s) 12, 317, 357, 483, 674, 845, 996, 1018
HpyF10VI GCNNNNNNNGC 4 cut(s) 911, 920, 929, 950
HpyF3I CTNAG 3 cut(s) 869, 939, 981
HpySE526I ACGT 2 cut(s) 717, 864
Hsp92II CATG 4 cut(s) 127, 163, 221, 742
HspAI GCGC 1 cut(s) 912
Ksp22I TGATCA 1 cut(s) 298
Kzo9I GATC 4 cut(s) 298, 523, 610, 826
LmnI GCTCC 1 cut(s) 431
Lsp1109I GCAGC 4 cut(s) 696, 932, 935, 956
LweI GCATC 1 cut(s) 789
MaeI CTAG 4 cut(s) 90, 563, 630, 734
MaeII ACGT 2 cut(s) 717, 864
MaeIII GTNAC 2 cut(s) 310, 934
MalI GATC 4 cut(s) 300, 525, 612, 828
MboI GATC 4 cut(s) 298, 523, 610, 826
MboII GAAGA 4 cut(s) 18, 86, 386, 976
MfeI CAATTG 1 cut(s) 599
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 2 cut(s) 234, 870
MluCI AATT 6 cut(s) 39, 224, 466, 570, 585, 599
MlyI GAGTC 2 cut(s) 250, 318
MmeI TCCRAC 1 cut(s) 846
MnlI CCTC 9 cut(s) 319, 514, 723, 734, 895, 921, 934, 954, 978
MroXI GAANNNNTTC 1 cut(s) 968
MseI TTAA 2 cut(s) 453, 588
MspI CCGG 3 cut(s) 235, 621, 728
MspR9I CCNGG 5 cut(s) 170, 236, 621, 728, 729
MunI CAATTG 1 cut(s) 599
MvaI CCWGG 1 cut(s) 170
MwoI GCNNNNNNNGC 4 cut(s) 911, 920, 929, 950
NciI CCSGG 4 cut(s) 236, 621, 728, 729
NcoI CCATGG 1 cut(s) 123
NdeII GATC 4 cut(s) 298, 523, 610, 826
NlaIII CATG 4 cut(s) 127, 163, 221, 742
NlaIV GGNNCC 4 cut(s) 112, 233, 337, 490
NmeAIII GCCGAG 1 cut(s) 349
NmuCI GTSAC 2 cut(s) 310, 934
PcsI WCGNNNNNNNCGW 1 cut(s) 831
PdmI GAANNNNTTC 1 cut(s) 968
PfeI GAWTC 4 cut(s) 86, 97, 377, 548
PflMI CCANNNNNTGG 1 cut(s) 897
PkrI GCNGC 4 cut(s) 711, 922, 925, 946
PleI GAGTC 2 cut(s) 249, 317
PpsI GAGTC 2 cut(s) 249, 317
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 4 cut(s) 112, 233, 337, 490
PstI CTGCAG 1 cut(s) 319
PsuI RGATCY 1 cut(s) 523
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
SaqAI TTAA 2 cut(s) 453, 588
SatI GCNGC 4 cut(s) 710, 921, 924, 945
Sau3AI GATC 4 cut(s) 298, 523, 610, 826
SchI GAGTC 2 cut(s) 250, 318
ScrFI CCNGG 5 cut(s) 170, 236, 621, 728, 729
SduI GDGCHC 2 cut(s) 234, 870
SfaNI GCATC 1 cut(s) 789
SfcI CTRYAG 1 cut(s) 315
SmaI CCCGGG 1 cut(s) 729
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
Sse9I AATT 6 cut(s) 39, 224, 466, 570, 585, 599
SsiI CCGC 3 cut(s) 660, 905, 932
SspI AATATT 1 cut(s) 1027
SspMI CTAG 4 cut(s) 90, 563, 630, 734
StyD4I CCNGG 5 cut(s) 168, 234, 619, 726, 727
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 3 cut(s) 812, 878, 1000
TaiI ACGT 2 cut(s) 720, 867
TaqI TCGA 3 cut(s) 55, 210, 664
TasI AATT 6 cut(s) 39, 224, 466, 570, 585, 599
TfiI GAWTC 4 cut(s) 86, 97, 377, 548
Tru1I TTAA 2 cut(s) 453, 588
Tru9I TTAA 2 cut(s) 453, 588
TscAI CASTG 6 cut(s) 319, 490, 499, 613, 883, 1003
TseFI GTSAC 2 cut(s) 310, 934
TseI GCWGC 4 cut(s) 709, 920, 923, 944
Tsp45I GTSAC 2 cut(s) 310, 934
TspDTI ATGAA 3 cut(s) 18, 107, 302
TspGWI ACGGA 1 cut(s) 597
TspMI CCCGGG 1 cut(s) 727
TspRI CASTG 6 cut(s) 319, 490, 499, 613, 883, 1003
Van91I CCANNNNNTGG 1 cut(s) 897
XapI RAATTY 3 cut(s) 39, 224, 466
XmaI CCCGGG 1 cut(s) 727
XmnI GAANNNNTTC 1 cut(s) 968
XspI CTAG 4 cut(s) 90, 563, 630, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.