FvH4_2g34310

RNA cap guanine-N2 methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014673
Physical Location & Seq
Reverse (-)
25522040 .. 25528130
6091 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34310.t1

Sequence Viewer

Length: 2313 bp
ATGGTTGAAGCTGAAGAGGAAGGGCCGGCTATGAGAGCTCTCGGATCTCTGTTGAAGCTCACCGAAGTCTTCCTCTGGGACGATGGCACGAGGAACGACATGTCGTTCTCTCTGAAACAGACTAAACCAGCTCAGGATGACGGAGACGATGACTCAAACGGCCTTGCCTCAAAAGGTGGAATTTTGCCTGAGGATATGGAACTTACGAGACAGATGAATGAATTGGGGCTTCCGGTGTCGTTTCAAACAAATAAACAGAAGCAGAAAAGGAAGGCTGGAGGCAAAAGAAAGGGAATGCGCTTGAAGCATCCGGACACTTGCCAAGACGTTGTGGGTGAAGCCCTGGACTCTTCCAAAGTGAGTGTGGAGGAGAGTGTATCTCCTATTGTATTTGATGATAATCCAAGCAGTTCTTTGTGTAGTATGTCGATGATGGGCCAAAGTGAATCATCTTGCTCTGATGTTGCAGTTGATGCCACCAAATTGCATTGCCCTTTTGGTGAAGGAGAAACACCAGCAAGTTTAATTGAGATCACTAGAGATTATGCCAAGGAACAACATGATGGAACACTCGGCACTGTTTCTGATGATGGTCACGATTGTGATTCCTTACACAACAGTGCTGTGGTCACTGATGCAGCAAAATTTTTAGCGAATTCAGTTAGCTGCTCAGCAGATGCTGCTACTGGCCATGGAAAGACAGAGCCAGATGTAAGACTGATGGAGCATGACCACTCAGAGTGTTCAGTATTGGCTTGCCATGAAGGAGAACTTACAACTGGTGAGGATCATGTCCCTGAGAAGCCATTTGTATCTGAATCAGTCTCATATTCCACATGTCCAGTAGTTCTTGACAATGATGGAATCGACGGCCAAAAAAATGCTGATTTTGGAGACTGGATGGTATATTGGGATTCCTACTACATGAGAAATTACTTCCATAATATGAGAACACATACTTCCACATGGTACCCACCTGAGGTCATGGAACATTTAGAAATTGTCGACACCACATGTAAGTCAAATGAAGGTGTTGCTGAAGTAACTGAGATAGATGTCACCACTTACTTGAAGAAAACAGGTTTATGTGGTCTGGATAAAATTGATTCATTTGAAGAACCAAGAAATGATGATGCAATACACAGTCAACCATATGATGAACTCACTGTTGTCAATTCTATGTTTGATACATCCACGTCAACTGTCACTGTAGGCATATGTCATGAGCTTCAAAGCAATAACAGCTTCATTGATGAATATGCATCATGCTTGTCATCAAATGTCCAGTGCCACATCTCTAGTTGCAGGAACCAGACCATGCCGCTGGTTTCTGATGAGGTGTGCAACAATGATCTGCTTGCAAGAAAATGTGATGAACCATATACTTATGATATCTTTGATAAACCTCATGACGTTCACTCTTGCGAGGAAATCCCTATATATTGTGAACATGATGAGGCCATTAGAACATTAGACTCACTCAGCATGTCCAATTCACGCACTGAAGAAGTTGAGGATAGGAGTATGCATTCTGAAACTGGAGTTCCTGCAACAAATGATTTGGTAATAAAATCTGACCGTGCTGTAGTTAAACGGAAAAAGAAAGTGAGAAGAATTCGAAGTCACAGAAAATTCTTCAATGAGGATGAAGAAGTTCTATTTGAAGGATTGTTGAAGGAGTGTTCTGCTGATATTGGTAAATATTGGTGTCAGAGGTATCTATTATTCTCCAGATATGATGATGGTATAAAAATGGACGAGGAAGGATGGTTTTCAGTTACTCCAGAAGCTCTAGCTAGGCATCATGCCCAACGTTGTGGTAGTGGCATCGTCATTGACTGTTTTACTGGAGTTGGTGGGAATGCCATCCAATTTGCTCAGATAAGCCAACATGTAACTGCCATTGATATTGATCCAACAAAAATTGATTATGCTCAACATAATGCTGCTATCTATGGAGTAGATGAAAGGATAGATTTCATTGAGGGGGACTTTTTCCGTTTGGCATCAAGGTTGAAGGCGGATACAGTCTTTTTGTCACCCCCATGGGGAGGACCTGATTATGCAAGAGAAGAGACTTATGACATTATGACAATGCTTAAGCCATATGATGGATATTTTCTCTTCAACACTGCAAAGCAAGTTGCTTCCAAGATTGTCATGTTTCTCCCTAGAAATGTTGATGTCAACCAATTAGCAGAGTTGTCTCTTTCAGTGACTCCGCCATGGTCGCTGGAGGTGGAGAAAAACTTTGTAAATGGCAAGTTGAAGGGAATAACTGCTTACTTCAGTGACACGGCAAGGAGAGAGTGA

Protein Analysis

771

Amino Acids

85.98

Weight (kDa)

4.72

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRM5-TYW2_MTfase PF02475 609 - 680 1e-07 TRM5/TYW2 methyltransferase domain
Methyltransf_15 PF09445 610 - 764 3.7e-45 RNA cap guanine-N2 methyltransferase
Methyltransf_31 PF13847 611 - 708 1e-06 Methyltransferase domain
Methyltransf_25 PF13649 611 - 679 5.8e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014673)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34310 FvH4_2g34310
malus_domestica MD15G1081500.v1.1
prunus_persica Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1
pyrus_communis pycom15g07620 pycom15g07630
rosa_chinensis RchiOBHm_Chr6g0308051
rosa_laevigata RLG00000010672
rosa_multiflora Rmu_sc0002624.1_g000003
rosa_roxburghii Rroxscaffold_7G00160630
rosa_rugosa Rorug06G0363400
rosa_samantha Rh6AG472300 Rh6BG483400 Rh6DG474700
rosa_wichuraiana Rw6G041220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 969
AccB1I GGYRCC 1 cut(s) 969
AccB7I CCANNNNNTGG 1 cut(s) 2111
AccI GTMKAC 1 cut(s) 1005
AccIII TCCGGA 1 cut(s) 310
AciI CCGC 3 cut(s) 1322, 2021, 2222
AclI AACGTT 1 cut(s) 1813
AclWI GGATC 3 cut(s) 52, 795, 1907
AcoI YGGCCR 2 cut(s) 688, 871
AcsI RAATTY 5 cut(s) 180, 644, 655, 1614, 1631
AcuI CTGAAG 4 cut(s) 33, 1059, 1524, 2272
AfaI GTAC 1 cut(s) 971
AfiI CCNNNNNNNGG 4 cut(s) 979, 2048, 2051, 2111
AflII CTTAAG 1 cut(s) 2099
AflIII ACRYGT 4 cut(s) 99, 836, 1013, 1891
AjiI CACGTC 1 cut(s) 1197
AjnI CCWGG 1 cut(s) 342
AleI CACNNNNGTG 2 cut(s) 600, 618
AluBI AGCT 9 cut(s) 11, 38, 58, 131, 666, 1228, 1245, 1790, 1796
AluI AGCT 9 cut(s) 11, 38, 58, 131, 666, 1228, 1245, 1790, 1796
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 6 cut(s) 138, 202, 829, 888, 2069, 2211
AlwI GGATC 3 cut(s) 52, 795, 1907
AlwNI CAGNNNCTG 1 cut(s) 680
Aor13HI TCCGGA 1 cut(s) 310
AoxI GGCC 6 cut(s) 23, 160, 436, 688, 871, 1458
ApeKI GCWGC 4 cut(s) 638, 666, 680, 1946
ApoI RAATTY 5 cut(s) 180, 644, 655, 1614, 1631
Asp700I GAANNNNTTC 1 cut(s) 1653
Asp718I GGTACC 1 cut(s) 969
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 3 cut(s) 23, 436, 2054
AsuHPI GGTGA 6 cut(s) 52, 347, 512, 794, 1051, 2031
AsuII TTCGAA 1 cut(s) 1618
AvaII GGWCC 1 cut(s) 2054
AxyI CCTNAGG 2 cut(s) 189, 978
BalI TGGCCA 1 cut(s) 690
BanI GGYRCC 1 cut(s) 969
BanII GRGCYC 1 cut(s) 40
BauI CACGAG 1 cut(s) 88
BbsI GAAGAC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 4 cut(s) 650, 653, 667, 1933
BceAI ACGGC 2 cut(s) 175, 886
BciT130I CCWGG 1 cut(s) 344
BciVI GTATCC 1 cut(s) 2017
BcoDI GTCTC 6 cut(s) 138, 202, 829, 888, 2069, 2211
BfaI CTAG 5 cut(s) 537, 1299, 1793, 1797, 2172
BfmI CTRYAG 2 cut(s) 1209, 1584
BfrI CTTAAG 1 cut(s) 2099
BfuI GTATCC 1 cut(s) 2017
BisI GCNGC 5 cut(s) 639, 667, 681, 1322, 1947
BlpI GCTNAGC 1 cut(s) 670
BlsI GCNGC 5 cut(s) 640, 668, 682, 1323, 1948
Bme1390I CCNGG 1 cut(s) 344
Bme18I GGWCC 1 cut(s) 2054
BmgBI CACGTC 1 cut(s) 1197
BmgT120I GGNCC 3 cut(s) 23, 436, 2054
BmiI GGNNCC 2 cut(s) 971, 1310
BmrFI CCNGG 1 cut(s) 344
BmsI GCATC 9 cut(s) 316, 463, 625, 667, 1123, 1271, 1810, 1836, 2015
BpiI GAAGAC 1 cut(s) 61
BplI GAGNNNNNCTC 2 cut(s) 364, 396
BpmI CTGGAG 6 cut(s) 297, 1560, 1714, 1767, 1869, 2255
Bpu10I CCTNAGC 1 cut(s) 132
Bpu1102I GCTNAGC 1 cut(s) 670
Bpu14I TTCGAA 1 cut(s) 1618
BsaBI GATNNNNATC 2 cut(s) 399, 1911
BsaJI CCNNGG 5 cut(s) 342, 549, 691, 2045, 2225
BsaWI WCCGGW 2 cut(s) 232, 310
Bsc4I CCNNNNNNNGG 4 cut(s) 979, 2048, 2051, 2111
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 7 cut(s) 691, 784, 842, 902, 1285, 1543, 1852
Bse21I CCTNAGG 2 cut(s) 189, 978
Bse3DI GCAATG 1 cut(s) 487
Bse8I GATNNNNATC 2 cut(s) 399, 1911
BseAI TCCGGA 1 cut(s) 310
BseBI CCWGG 1 cut(s) 344
BseDI CCNNGG 5 cut(s) 342, 549, 691, 2045, 2225
BseGI GGATG 7 cut(s) 142, 307, 906, 1190, 1651, 1772, 1866
BseJI GATNNNNATC 2 cut(s) 399, 1911
BseLI CCNNNNNNNGG 4 cut(s) 979, 2048, 2051, 2111
BseMI GCAATG 1 cut(s) 487
BseMII CTCAG 9 cut(s) 146, 180, 684, 750, 789, 969, 1038, 1495, 1892
BseNI ACTGG 7 cut(s) 691, 784, 842, 902, 1285, 1543, 1852
BseRI GAGGAG 1 cut(s) 383
BseXI GCAGC 4 cut(s) 650, 653, 667, 1933
BshFI GGCC 6 cut(s) 25, 162, 438, 690, 873, 1460
BshNI GGYRCC 1 cut(s) 969
BsiHKAI GWGCWC 1 cut(s) 40
BsiSI CCGG 3 cut(s) 26, 233, 311
BslFI GGGAC 3 cut(s) 92, 779, 2003
BslI CCNNNNNNNGG 4 cut(s) 979, 2048, 2051, 2111
BsmAI GTCTC 6 cut(s) 138, 202, 829, 888, 2069, 2211
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 3 cut(s) 92, 779, 2003
BsmI GAATGC 3 cut(s) 300, 1528, 1867
BsnI GGCC 6 cut(s) 25, 162, 438, 690, 873, 1460
Bsp119I TTCGAA 1 cut(s) 1618
Bsp1286I GDGCHC 1 cut(s) 40
Bsp13I TCCGGA 1 cut(s) 310
Bsp143I GATC 5 cut(s) 44, 531, 787, 1351, 1912
Bsp1720I GCTNAGC 1 cut(s) 670
Bsp19I CCATGG 3 cut(s) 691, 2045, 2225
BspACI CCGC 3 cut(s) 1322, 2021, 2222
BspANI GGCC 6 cut(s) 25, 162, 438, 690, 873, 1460
BspCNI CTCAG 9 cut(s) 145, 181, 683, 749, 790, 970, 1039, 1494, 1891
BspEI TCCGGA 1 cut(s) 310
BspHI TCATGA 2 cut(s) 1222, 1408
BspLI GGNNCC 2 cut(s) 971, 1310
BspPI GGATC 3 cut(s) 52, 795, 1907
BspT104I TTCGAA 1 cut(s) 1618
BspT107I GGYRCC 1 cut(s) 969
BspTI CTTAAG 1 cut(s) 2099
BsrDI GCAATG 1 cut(s) 487
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 7 cut(s) 691, 784, 842, 902, 1285, 1543, 1852
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 5 cut(s) 342, 549, 691, 2045, 2225
BssMI GATC 5 cut(s) 44, 531, 787, 1351, 1912
BssSI CACGAG 1 cut(s) 88
BssT1I CCWWGG 4 cut(s) 549, 691, 2045, 2225
Bst2BI CACGAG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 9 cut(s) 580, 620, 1145, 1168, 1204, 1210, 1580, 1841, 2029
Bst6I CTCTTC 4 cut(s) 9, 355, 2067, 2129
BstAFI CTTAAG 1 cut(s) 2099
BstAPI GCANNNNNTGC 2 cut(s) 473, 680
BstBI TTCGAA 1 cut(s) 1618
BstC8I GCNNGC 3 cut(s) 27, 757, 1359
BstDEI CTNAG 9 cut(s) 132, 189, 670, 736, 798, 978, 1047, 1481, 1878
BstDSI CCRYGG 3 cut(s) 691, 2045, 2225
BstF5I GGATG 7 cut(s) 142, 307, 906, 1190, 1651, 1772, 1866
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 5 cut(s) 47, 534, 790, 1354, 1915
BstMAI GTCTC 6 cut(s) 138, 202, 829, 888, 2069, 2211
BstMBI GATC 5 cut(s) 44, 531, 787, 1351, 1912
BstMWI GCNNNNNNNGC 6 cut(s) 35, 304, 473, 680, 1242, 2230
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 5 cut(s) 103, 840, 1017, 1489, 1895
BstSCI CCNGG 1 cut(s) 342
BstSFI CTRYAG 2 cut(s) 1209, 1584
BstV1I GCAGC 4 cut(s) 650, 653, 667, 1933
BstV2I GAAGAC 1 cut(s) 61
BstX2I RGATCY 1 cut(s) 44
BstXI CCANNNNNNTGG 2 cut(s) 1324, 1817
BstYI RGATCY 1 cut(s) 44
Bsu36I CCTNAGG 2 cut(s) 189, 978
BsuI GTATCC 1 cut(s) 2017
BsuRI GGCC 6 cut(s) 25, 162, 438, 690, 873, 1460
BtgI CCRYGG 3 cut(s) 691, 2045, 2225
BtrI CACGTC 1 cut(s) 1197
BtsCI GGATG 7 cut(s) 142, 307, 906, 1190, 1651, 1772, 1866
BtsI GCAGTG 1 cut(s) 2130
Cac8I GCNNGC 3 cut(s) 27, 757, 1359
CaiI CAGNNNCTG 1 cut(s) 680
CciI TCATGA 2 cut(s) 1222, 1408
CfoI GCGC 1 cut(s) 300
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 3 cut(s) 23, 436, 2054
Csp6I GTAC 1 cut(s) 970
CviQI GTAC 1 cut(s) 970
DdeI CTNAG 9 cut(s) 132, 189, 670, 736, 798, 978, 1047, 1481, 1878
DpnI GATC 5 cut(s) 46, 533, 789, 1353, 1914
DpnII GATC 5 cut(s) 44, 531, 787, 1351, 1912
EaeI YGGCCR 2 cut(s) 688, 871
Eam1104I CTCTTC 4 cut(s) 9, 355, 2067, 2129
EarI CTCTTC 4 cut(s) 9, 355, 2067, 2129
EciI GGCGGA 2 cut(s) 2036, 2211
Ecl136II GAGCTC 1 cut(s) 38
Eco130I CCWWGG 4 cut(s) 549, 691, 2045, 2225
Eco24I GRGCYC 1 cut(s) 40
Eco32I GATATC 1 cut(s) 1393
Eco47I GGWCC 1 cut(s) 2054
Eco53kI GAGCTC 1 cut(s) 38
Eco57I CTGAAG 4 cut(s) 33, 1059, 1524, 2272
Eco81I CCTNAGG 2 cut(s) 189, 978
EcoICRI GAGCTC 1 cut(s) 38
EcoO109I RGGNCCY 1 cut(s) 2054
EcoRI GAATTC 2 cut(s) 655, 1614
EcoRII CCWGG 1 cut(s) 342
EcoRV GATATC 1 cut(s) 1393
EcoT14I CCWWGG 4 cut(s) 549, 691, 2045, 2225
EcoT22I ATGCAT 2 cut(s) 1264, 1530
EcoT38I GRGCYC 1 cut(s) 40
ErhI CCWWGG 4 cut(s) 549, 691, 2045, 2225
Esp3I CGTCTC 1 cut(s) 138
FalI AAGNNNNNCTT 4 cut(s) 397, 429, 756, 788
FaqI GGGAC 3 cut(s) 92, 779, 2003
FauNDI CATATG 3 cut(s) 1153, 1217, 2107
FblI GTMKAC 1 cut(s) 1005
Fnu4HI GCNGC 5 cut(s) 639, 667, 681, 1322, 1947
FokI GGATG 7 cut(s) 149, 294, 913, 1177, 1658, 1779, 1853
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 5 cut(s) 639, 667, 681, 1322, 1947
FspBI CTAG 5 cut(s) 537, 1299, 1793, 1797, 2172
GlaI GCGC 1 cut(s) 299
GluI GCNGC 5 cut(s) 639, 667, 681, 1322, 1947
GsuI CTGGAG 6 cut(s) 297, 1560, 1714, 1767, 1869, 2255
HaeIII GGCC 6 cut(s) 25, 162, 438, 690, 873, 1460
HapII CCGG 3 cut(s) 26, 233, 311
HhaI GCGC 1 cut(s) 300
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HincII GTYRAC 4 cut(s) 1006, 1148, 1200, 2188
HindII GTYRAC 4 cut(s) 1006, 1148, 1200, 2188
HpaII CCGG 3 cut(s) 26, 233, 311
HphI GGTGA 6 cut(s) 52, 347, 512, 794, 1051, 2031
Hpy166II GTNNAC 6 cut(s) 1006, 1148, 1200, 1417, 1448, 2188
Hpy188III TCNNGA 9 cut(s) 134, 311, 596, 851, 1094, 1223, 1409, 1731, 1784
Hpy8I GTNNAC 6 cut(s) 1006, 1148, 1200, 1417, 1448, 2188
Hpy99I CGWCG 1 cut(s) 872
HpyCH4III ACNGT 9 cut(s) 580, 620, 1145, 1168, 1204, 1210, 1580, 1841, 2029
HpyCH4IV ACGT 4 cut(s) 327, 1196, 1413, 1813
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 304, 473, 680, 1242, 2230
HpyF3I CTNAG 9 cut(s) 132, 189, 670, 736, 798, 978, 1047, 1481, 1878
HpySE526I ACGT 4 cut(s) 327, 1196, 1413, 1813
HspAI GCGC 1 cut(s) 298
Kpn2I TCCGGA 1 cut(s) 310
KpnI GGTACC 1 cut(s) 973
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Kzo9I GATC 5 cut(s) 44, 531, 787, 1351, 1912
LmnI GCTCC 1 cut(s) 724
Lsp1109I GCAGC 4 cut(s) 650, 653, 667, 1933
LweI GCATC 9 cut(s) 316, 463, 625, 667, 1123, 1271, 1810, 1836, 2015
MaeI CTAG 5 cut(s) 537, 1299, 1793, 1797, 2172
MaeII ACGT 4 cut(s) 327, 1196, 1413, 1813
MalI GATC 5 cut(s) 46, 533, 789, 1353, 1914
MboI GATC 5 cut(s) 44, 531, 787, 1351, 1912
MflI RGATCY 1 cut(s) 44
MhlI GDGCHC 1 cut(s) 40
MlsI TGGCCA 1 cut(s) 690
MluNI TGGCCA 1 cut(s) 690
MlyI GAGTC 4 cut(s) 146, 341, 1469, 2212
MmeI TCCRAC 1 cut(s) 1940
Mox20I TGGCCA 1 cut(s) 690
Mph1103I ATGCAT 2 cut(s) 1264, 1530
MroI TCCGGA 1 cut(s) 310
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 1 cut(s) 1653
MscI TGGCCA 1 cut(s) 690
MseI TTAA 3 cut(s) 524, 1590, 2100
MslI CAYNNNNRTG 3 cut(s) 600, 618, 2044
Msp20I TGGCCA 1 cut(s) 690
MspA1I CMGCKG 1 cut(s) 1324
MspCI CTTAAG 1 cut(s) 2099
MspI CCGG 3 cut(s) 26, 233, 311
MspR9I CCNGG 1 cut(s) 344
Mva1269I GAATGC 3 cut(s) 300, 1528, 1867
MvaI CCWGG 1 cut(s) 344
MwoI GCNNNNNNNGC 6 cut(s) 35, 304, 473, 680, 1242, 2230
NaeI GCCGGC 1 cut(s) 27
NcoI CCATGG 3 cut(s) 691, 2045, 2225
NdeI CATATG 3 cut(s) 1153, 1217, 2107
NdeII GATC 5 cut(s) 44, 531, 787, 1351, 1912
NgoMIV GCCGGC 1 cut(s) 25
NlaIV GGNNCC 2 cut(s) 971, 1310
NmeAIII GCCGAG 1 cut(s) 552
NmuCI GTSAC 8 cut(s) 593, 628, 1057, 1204, 1622, 2037, 2215, 2291
NsiI ATGCAT 2 cut(s) 1264, 1530
NspI RCATGY 5 cut(s) 103, 840, 1017, 1489, 1895
NspV TTCGAA 1 cut(s) 1618
OliI CACNNNNGTG 2 cut(s) 600, 618
PagI TCATGA 2 cut(s) 1222, 1408
PciI ACATGT 4 cut(s) 99, 836, 1013, 1891
PctI GAATGC 3 cut(s) 300, 1528, 1867
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 1653
PfeI GAWTC 6 cut(s) 446, 605, 818, 864, 914, 1106
PflMI CCANNNNNTGG 1 cut(s) 2111
PkrI GCNGC 5 cut(s) 640, 668, 682, 1323, 1948
PleI GAGTC 4 cut(s) 146, 341, 1469, 2212
PpsI GAGTC 4 cut(s) 146, 341, 1469, 2212
PpuMI RGGWCCY 1 cut(s) 2054
PscI ACATGT 4 cut(s) 99, 836, 1013, 1891
Psp124BI GAGCTC 1 cut(s) 40
Psp1406I AACGTT 1 cut(s) 1813
Psp5II RGGWCCY 1 cut(s) 2054
Psp6I CCWGG 1 cut(s) 342
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 2 cut(s) 971, 1310
PspPI GGNCC 3 cut(s) 23, 436, 2054
PspPPI RGGWCCY 1 cut(s) 2054
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 1 cut(s) 44
RsaI GTAC 1 cut(s) 971
RsaNI GTAC 1 cut(s) 970
RseI CAYNNNNRTG 3 cut(s) 600, 618, 2044
SacI GAGCTC 1 cut(s) 40
SalI GTCGAC 1 cut(s) 1004
SaqAI TTAA 3 cut(s) 524, 1590, 2100
SatI GCNGC 5 cut(s) 639, 667, 681, 1322, 1947
Sau3AI GATC 5 cut(s) 44, 531, 787, 1351, 1912
Sau96I GGNCC 3 cut(s) 23, 436, 2054
SchI GAGTC 4 cut(s) 146, 341, 1469, 2212
ScrFI CCNGG 1 cut(s) 344
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 9 cut(s) 316, 463, 625, 667, 1123, 1271, 1810, 1836, 2015
SfcI CTRYAG 2 cut(s) 1209, 1584
SfuI TTCGAA 1 cut(s) 1618
SinI GGWCC 1 cut(s) 2054
SmiMI CAYNNNNRTG 3 cut(s) 600, 618, 2044
SmlI CTYRAG 1 cut(s) 2099
SmoI CTYRAG 1 cut(s) 2099
SsiI CCGC 3 cut(s) 1322, 2021, 2222
SspI AATATT 1 cut(s) 1703
SspMI CTAG 5 cut(s) 537, 1299, 1793, 1797, 2172
SstI GAGCTC 1 cut(s) 40
StyD4I CCNGG 1 cut(s) 342
StyI CCWWGG 4 cut(s) 549, 691, 2045, 2225
TaaI ACNGT 9 cut(s) 580, 620, 1145, 1168, 1204, 1210, 1580, 1841, 2029
TaiI ACGT 4 cut(s) 330, 1199, 1416, 1816
TaqI TCGA 4 cut(s) 428, 867, 1005, 1618
TauI GCSGC 1 cut(s) 1324
TfiI GAWTC 6 cut(s) 446, 605, 818, 864, 914, 1106
Tru1I TTAA 3 cut(s) 524, 1590, 2100
Tru9I TTAA 3 cut(s) 524, 1590, 2100
TseFI GTSAC 8 cut(s) 593, 628, 1057, 1204, 1622, 2037, 2215, 2291
TseI GCWGC 4 cut(s) 638, 666, 680, 1946
Tsp45I GTSAC 8 cut(s) 593, 628, 1057, 1204, 1622, 2037, 2215, 2291
TspGWI ACGGA 3 cut(s) 156, 1609, 1988
Van91I CCANNNNNTGG 1 cut(s) 2111
Vha464I CTTAAG 1 cut(s) 2099
VpaK11BI GGWCC 1 cut(s) 2054
XapI RAATTY 5 cut(s) 180, 644, 655, 1614, 1631
XceI RCATGY 5 cut(s) 103, 840, 1017, 1489, 1895
XcmI CCANNNNNNNNNTGG 1 cut(s) 361
XmiI GTMKAC 1 cut(s) 1005
XmnI GAANNNNTTC 1 cut(s) 1653
XspI CTAG 5 cut(s) 537, 1299, 1793, 1797, 2172
Zsp2I ATGCAT 2 cut(s) 1264, 1530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.