Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0014673

Physical Location & Seq

Genomic Coordinates Forward (+)

64925256 .. 64929518

Gene Structure 4263 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G041220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2379 bp

ATGGTTGAAGCTGAAGACGAGGGGCCTGCTGTGAGAGCTCTCGGATCTCTGTTGAAGCTCACCCAAGTCTTTCTCTGGGACGATGGCACAAGAAACGACTGGTCGTTTTCCCTGGGACAGACTAAACCAGCTCAGGATGAAGGAGACGATGGCTCAAACGCTCTTTCCTCAGGCTATTGGAATTTCTGGGTTTCAGAATGTGGAACTTTGCCGGAGGATATGGAACTTACTAGACAGATGAATGAGTTGGGGCTTCCGGTCTCATTTCGCACAAATAAGGAGAAGCAGAAGAGAACAACTCAAGGAAAAAGAAAAGGAATGCGCCAGAAGCATTCGAACGAATCTCAAGAAGTTGTGGGTGGAGCCCTGGACTCTTCCAAAGTGAGTGTGGAGGAGATTGTATCTCCTATTATATTTGATGATAACCCAAGCAGTTCTTTGTGTTGTATGTCAATGATGGGCCAAAGTGAATCATCTTCCTCTGATGTTGCAGCTGATGCTAATAATCCAGCAAGTTCAGCTAAGATTACTGGTGATTCTATTAAGGAACATAAGCGTGATGGAATAGTCGGCATTGTTTCTAATGAAGGCCAGGATTGTGATTCCTTACACAATGGTGCCATGCTCACTGATGCAACAACAATTTTAGCAAGTTCAACTGGGTTAGATGCTGTACTTTACCCAGGCAGCTGCTCTGCAGATGCTGCTAATGGCTATGATAAGACGGAGCCAGGTGAAAGACTTATGGAGCATGACCACGTAGAGTGTGCAATAATGGCTATCGGTGAAGCAGAAATAACCGAAAGTTGTGAGGAGCATGTCCCTGAGCAGCCGTGTGTTTCTGAATCAGTCTCATGTTCCATATATCCAGAAGGTCTTGAGAATGATGCAATTGACAGCCAACAAAGTGGTGATTTTGGAGACTGGATGGTATATTGGGATTCTTACTACATGAGAAATTACTTCTATAATATTAGAACACATACTTCAACATGGTACCCACCCATGGGCATGGAACATTTAGAAAATGTTGATAGCACATATAAGTCAAATGAAGGTGTTGCTGGAGTAACTCATATGGATGTTACCACAGACTTAAAGGCAACAGATTTATGTGTTTTGAGTAAGTTTAACTCATTTGAAGAACCAATAAATGTTGATGTTTTACAATATCAACGATATGAAGAGGTTTCAAGGGATGTTGAAATTACTTCTGCCAATACTTTATCGGAAACATCCACTTCAACTGTCAATGTAAGCATATGTGCTGAGCTTCCAGTTGAACTTCAGAGCAATAACAGGTGCAATGATGGAAATGCATCATGCTTGTCATCAAATGTCCAGGACCACATTGCTAGTTGCAGAAACCAAACCATGGAGCAGGTTGCTGATGAGGTCTGCAACAGTGATTTGCAACCAATTTTTGCAGGAAAAAGTGCTGAACCAGATATTTCTGATCTCTATGATAAACCTAATGAAGTTGACTTTTGTGAGGAAATTCCTAAATATGGTGAACATGATGAGGTGATTCAAACATTAGATACAAGCAGCATGTCCAATTCATGCACTGAAGAAGTTGAGGATTGGAATATGCATTCTGAAACTGGAGTTCCTGCAACAAATGAGTTGGAAATGAAATCTGACCCTGCTTTAGTAAAACGGAAAAAGAAAGTGAGAAGAATTCGAAGTCACAGAAAATTCTTCAACGAGAATGAAGAAGTTCTATTTGAAGGATTGCTGAAGAAGTGTTCCACTGATATTGGTAAATATTGGTCTCAGAGATATCTATTATTCTCCAGATATGATGATGGTATAAAAATGGATGAGGAAGGATGGTTTTCAGTTACTCCAGAAGCTTTAGCTATGCATCATGCCCAACGTTGTGGTAGTGGCATTATAATTGACTGTTTTACTGGAGTTGGTGGTAATGCCATCCAATTTGCTCAGATAAGCAAACATGTAATTGCCATTGATATTGATCCAACAAAGATTGATTATGCTCAACATAATGCTGCTATCTACGGAGTAGACGAAAGGATAGATTTCATAAAGGGGGACTTTTTCCATTTGGCATCAAGGTTGAAGGCAGATACAGTCTTTTTGTCACCACCATGGGGAGGACCTGGTTATGCAAGAGAAGAGACATATGACATTATGACAATGCTTAAGCCACATGATGGATATTTTCTCTTCAACACTGCTAAGCAAGTTGCTTCCAAGATTGTCATGTTTCTCCCAAGAAATGTTGATGTCAACCAATTAGCAGAGTTGTCTCTTTCAGGGACTCCACCATGGTCGCTAGAGGTGGAGAAAAACTTTGTAAATGGCAAGTTGAAGGGAATAACTGCTTACTTCAGTGACACGGCAAGGAGAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016741 GO:0032259 GO:0034641 GO:0036260 GO:0036261 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071164 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Methyltransf_15 WW

Protein Analysis

792

Amino Acids

87.97

Weight (kDa)

4.59

Isoelectric Point (pI)

42.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_15	PF09445	632 - 786	1.4e-43	RNA cap guanine-N2 methyltransferase
TRM5-TYW2_MTfase	PF02475	632 - 702	1.1e-07	TRM5/TYW2 methyltransferase domain
Methyltransf_31	PF13847	633 - 735	1.6e-07	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014673)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34310 FvH4_2g34310
malus_domestica	MD15G1081500.v1.1
prunus_persica	Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1
pyrus_communis	pycom15g07620 pycom15g07630
rosa_chinensis	RchiOBHm_Chr6g0308051
rosa_laevigata	RLG00000010672
rosa_multiflora	Rmu_sc0002624.1_g000003
rosa_roxburghii	Rroxscaffold_7G00160630
rosa_rugosa	Rorug06G0363400
rosa_samantha	Rh6AG472300 Rh6BG483400 Rh6DG474700
rosa_wichuraiana	Rw6G041220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1898
Acc36I	ACCTGC	1 cut(s)	1372
Acc65I	GGTACC	1 cut(s)	996
AccB1I	GGYRCC	2 cut(s)	617, 996
AccB7I	CCANNNNNTGG	2 cut(s)	1375, 2177
AccI	GTMKAC	1 cut(s)	2028
AclI	AACGTT	1 cut(s)	1879
AclWI	GGATC	2 cut(s)	52, 1973
AcsI	RAATTY	4 cut(s)	181, 1497, 1680, 1697
AcuI	CTGAAG	5 cut(s)	33, 1271, 1590, 1760, 2338
AfaI	GTAC	2 cut(s)	675, 998
AfiI	CCNNNNNNNGG	7 cut(s)	1006, 1007, 1375, 1508, 2114, 2117, 2177
AflII	CTTAAG	1 cut(s)	2165
AflIII	ACRYGT	1 cut(s)	1957
AjnI	CCWGG	7 cut(s)	111, 366, 591, 682, 730, 1341, 2122
AleI	CACNNNNGTG	1 cut(s)	615
Alw21I	GWGCWC	1 cut(s)	40
Alw26I	GTCTC	7 cut(s)	138, 265, 856, 915, 1779, 2135, 2277
AlwI	GGATC	2 cut(s)	52, 1973
AlwNI	CAGNNNCTG	1 cut(s)	704
AoxI	GGCC	3 cut(s)	23, 460, 589
ApeKI	GCWGC	7 cut(s)	491, 687, 690, 704, 829, 1548, 2012
ApoI	RAATTY	4 cut(s)	181, 1497, 1680, 1697
Asp700I	GAANNNNTTC	1 cut(s)	1719
Asp718I	GGTACC	1 cut(s)	996
AspLEI	GCGC	1 cut(s)	324
AspS9I	GGNCC	4 cut(s)	23, 460, 1345, 2120
AsuHPI	GGTGA	8 cut(s)	52, 545, 746, 797, 923, 1523, 1537, 2097
AsuII	TTCGAA	2 cut(s)	335, 1684
AvaII	GGWCC	2 cut(s)	1345, 2120
AxyI	CCTNAGG	1 cut(s)	169
BanI	GGYRCC	2 cut(s)	617, 996
BanII	GRGCYC	2 cut(s)	40, 367
BbsI	GAAGAC	1 cut(s)	21
Bbv12I	GWGCWC	1 cut(s)	40
BbvI	GCAGC	7 cut(s)	503, 677, 691, 699, 841, 1560, 1999
BceAI	ACGGC	1 cut(s)	817
BcgI	CGANNNNNNTGC	2 cut(s)	8, 42
BciT130I	CCWGG	7 cut(s)	113, 368, 593, 684, 732, 1343, 2124
BcoDI	GTCTC	7 cut(s)	138, 265, 856, 915, 1779, 2135, 2277
BfaI	CTAG	3 cut(s)	231, 1356, 2300
BfmI	CTRYAG	1 cut(s)	696
BfrI	CTTAAG	1 cut(s)	2165
BfuAI	ACCTGC	1 cut(s)	1372
BisI	GCNGC	7 cut(s)	492, 688, 691, 705, 830, 1549, 2013
BlpI	GCTNAGC	2 cut(s)	1269, 2202
BlsI	GCNGC	7 cut(s)	493, 689, 692, 706, 831, 1550, 2014
Bme1390I	CCNGG	7 cut(s)	113, 368, 593, 684, 732, 1343, 2124
Bme18I	GGWCC	2 cut(s)	1345, 2120
BmgT120I	GGNCC	4 cut(s)	23, 460, 1345, 2120
BmiI	GGNNCC	5 cut(s)	24, 364, 619, 729, 998
BmrFI	CCNGG	7 cut(s)	113, 368, 593, 684, 732, 1343, 2124
BmrI	ACTGGG	1 cut(s)	669
BmsI	GCATC	8 cut(s)	487, 622, 658, 691, 877, 1328, 1876, 2081
BmuI	ACTGGG	1 cut(s)	669
BpiI	GAAGAC	1 cut(s)	21
BplI	GAGNNNNNCTC	2 cut(s)	283, 315
BpmI	CTGGAG	5 cut(s)	1086, 1626, 1780, 1833, 1935
Bpu10I	CCTNAGC	2 cut(s)	132, 825
Bpu1102I	GCTNAGC	2 cut(s)	1269, 2202
Bpu14I	TTCGAA	2 cut(s)	335, 1684
BpuEI	CTTGAG	3 cut(s)	285, 330, 899
BsaAI	YACGTR	1 cut(s)	760
BsaBI	GATNNNNATC	1 cut(s)	1977
BsaI	GGTCTC	2 cut(s)	265, 1779
BsaJI	CCNNGG	8 cut(s)	111, 112, 366, 682, 1005, 1374, 2111, 2291
BsaWI	WCCGGW	1 cut(s)	256
Bsc4I	CCNNNNNNNGG	7 cut(s)	1006, 1007, 1375, 1508, 2114, 2117, 2177
Bse1I	ACTGG	7 cut(s)	104, 535, 664, 929, 1277, 1609, 1918
Bse21I	CCTNAGG	1 cut(s)	169
Bse3DI	GCAATG	2 cut(s)	1312, 1350
Bse8I	GATNNNNATC	1 cut(s)	1977
BseBI	CCWGG	7 cut(s)	113, 368, 593, 684, 732, 1343, 2124
BseDI	CCNNGG	8 cut(s)	111, 112, 366, 682, 1005, 1374, 2111, 2291
BseGI	GGATG	8 cut(s)	142, 933, 1087, 1204, 1235, 1828, 1838, 1932
BseJI	GATNNNNATC	1 cut(s)	1977
BseLI	CCNNNNNNNGG	7 cut(s)	1006, 1007, 1375, 1508, 2114, 2117, 2177
BseMI	GCAATG	2 cut(s)	1312, 1350
BseMII	CTCAG	6 cut(s)	146, 183, 816, 1260, 1790, 1958
BseNI	ACTGG	7 cut(s)	104, 535, 664, 929, 1277, 1609, 1918
BseRI	GAGGAG	2 cut(s)	407, 827
BseXI	GCAGC	7 cut(s)	503, 677, 691, 699, 841, 1560, 1999
BshFI	GGCC	3 cut(s)	25, 462, 591
BshNI	GGYRCC	2 cut(s)	617, 996
BsiHKAI	GWGCWC	1 cut(s)	40
BsiSI	CCGG	2 cut(s)	212, 257
BslFI	GGGAC	5 cut(s)	92, 129, 806, 2069, 2296
BslI	CCNNNNNNNGG	7 cut(s)	1006, 1007, 1375, 1508, 2114, 2117, 2177
BsmAI	GTCTC	7 cut(s)	138, 265, 856, 915, 1779, 2135, 2277
BsmBI	CGTCTC	1 cut(s)	138
BsmFI	GGGAC	5 cut(s)	92, 129, 806, 2069, 2296
BsmI	GAATGC	3 cut(s)	324, 331, 1594
BsnI	GGCC	3 cut(s)	25, 462, 591
Bso31I	GGTCTC	2 cut(s)	265, 1779
Bsp119I	TTCGAA	2 cut(s)	335, 1684
Bsp1286I	GDGCHC	2 cut(s)	40, 367
Bsp143I	GATC	3 cut(s)	44, 1456, 1978
Bsp1720I	GCTNAGC	2 cut(s)	1269, 2202
Bsp19I	CCATGG	4 cut(s)	1005, 1374, 2111, 2291
BspANI	GGCC	3 cut(s)	25, 462, 591
BspCNI	CTCAG	6 cut(s)	145, 182, 817, 1261, 1789, 1957
BspLI	GGNNCC	5 cut(s)	24, 364, 619, 729, 998
BspMAI	CTGCAG	1 cut(s)	700
BspMI	ACCTGC	1 cut(s)	1372
BspPI	GGATC	2 cut(s)	52, 1973
BspT104I	TTCGAA	2 cut(s)	335, 1684
BspT107I	GGYRCC	2 cut(s)	617, 996
BspTI	CTTAAG	1 cut(s)	2165
BspTNI	GGTCTC	2 cut(s)	265, 1779
BsrDI	GCAATG	2 cut(s)	1312, 1350
BsrI	ACTGG	7 cut(s)	104, 535, 664, 929, 1277, 1609, 1918
BssECI	CCNNGG	8 cut(s)	111, 112, 366, 682, 1005, 1374, 2111, 2291
BssMI	GATC	3 cut(s)	44, 1456, 1978
BssT1I	CCWWGG	4 cut(s)	1005, 1374, 2111, 2291
Bst2UI	CCWGG	7 cut(s)	113, 368, 593, 684, 732, 1343, 2124
Bst4CI	ACNGT	4 cut(s)	1249, 1406, 1907, 2095
Bst6I	CTCTTC	5 cut(s)	284, 379, 1179, 2133, 2195
BstAFI	CTTAAG	1 cut(s)	2165
BstAPI	GCANNNNNTGC	2 cut(s)	497, 704
BstBAI	YACGTR	1 cut(s)	760
BstBI	TTCGAA	2 cut(s)	335, 1684
BstC8I	GCNNGC	1 cut(s)	27
BstDEI	CTNAG	8 cut(s)	132, 169, 522, 825, 1269, 1776, 1944, 2202
BstDSI	CCRYGG	4 cut(s)	1005, 1374, 2111, 2291
BstF5I	GGATG	8 cut(s)	142, 933, 1087, 1204, 1235, 1828, 1838, 1932
BstHHI	GCGC	1 cut(s)	324
BstKTI	GATC	3 cut(s)	47, 1459, 1981
BstMAI	GTCTC	7 cut(s)	138, 265, 856, 915, 1779, 2135, 2277
BstMBI	GATC	3 cut(s)	44, 1456, 1978
BstMWI	GCNNNNNNNGC	6 cut(s)	35, 328, 497, 518, 704, 776
BstNI	CCWGG	7 cut(s)	113, 368, 593, 684, 732, 1343, 2124
BstNSI	RCATGY	3 cut(s)	821, 1555, 1961
BstSCI	CCNGG	7 cut(s)	111, 366, 591, 682, 730, 1341, 2122
BstSFI	CTRYAG	1 cut(s)	696
BstV1I	GCAGC	7 cut(s)	503, 677, 691, 699, 841, 1560, 1999
BstV2I	GAAGAC	1 cut(s)	21
BstX2I	RGATCY	1 cut(s)	44
BstXI	CCANNNNNNTGG	3 cut(s)	908, 1012, 1883
BstYI	RGATCY	1 cut(s)	44
Bsu36I	CCTNAGG	1 cut(s)	169
BsuRI	GGCC	3 cut(s)	25, 462, 591
BtgI	CCRYGG	4 cut(s)	1005, 1374, 2111, 2291
BtsCI	GGATG	8 cut(s)	142, 933, 1087, 1204, 1235, 1828, 1838, 1932
BtsI	GCAGTG	1 cut(s)	2196
BtsIMutI	CAGTG	6 cut(s)	627, 1411, 1566, 1752, 2196, 2362
BveI	ACCTGC	1 cut(s)	1372
Cac8I	GCNNGC	1 cut(s)	27
CaiI	CAGNNNCTG	1 cut(s)	704
CfoI	GCGC	1 cut(s)	324
Cfr13I	GGNCC	4 cut(s)	23, 460, 1345, 2120
CsiI	ACCWGGT	1 cut(s)	2122
Csp6I	GTAC	2 cut(s)	674, 997
CspCI	CAANNNNNGTGG	2 cut(s)	1741, 1776
CviQI	GTAC	2 cut(s)	674, 997
DdeI	CTNAG	8 cut(s)	132, 169, 522, 825, 1269, 1776, 1944, 2202
DpnI	GATC	3 cut(s)	46, 1458, 1980
DpnII	GATC	3 cut(s)	44, 1456, 1978
Eam1104I	CTCTTC	5 cut(s)	284, 379, 1179, 2133, 2195
EarI	CTCTTC	5 cut(s)	284, 379, 1179, 2133, 2195
Ecl136II	GAGCTC	1 cut(s)	38
Eco130I	CCWWGG	4 cut(s)	1005, 1374, 2111, 2291
Eco24I	GRGCYC	2 cut(s)	40, 367
Eco31I	GGTCTC	2 cut(s)	265, 1779
Eco32I	GATATC	1 cut(s)	1784
Eco47I	GGWCC	2 cut(s)	1345, 2120
Eco53kI	GAGCTC	1 cut(s)	38
Eco57I	CTGAAG	5 cut(s)	33, 1271, 1590, 1760, 2338
Eco81I	CCTNAGG	1 cut(s)	169
EcoICRI	GAGCTC	1 cut(s)	38
EcoO109I	RGGNCCY	2 cut(s)	23, 2120
EcoRI	GAATTC	1 cut(s)	1680
EcoRII	CCWGG	7 cut(s)	111, 366, 591, 682, 730, 1341, 2122
EcoRV	GATATC	1 cut(s)	1784
EcoT14I	CCWWGG	4 cut(s)	1005, 1374, 2111, 2291
EcoT22I	ATGCAT	3 cut(s)	1321, 1596, 1869
EcoT38I	GRGCYC	2 cut(s)	40, 367
ErhI	CCWWGG	4 cut(s)	1005, 1374, 2111, 2291
Esp3I	CGTCTC	1 cut(s)	138
FalI	AAGNNNNNCTT	4 cut(s)	421, 453, 2042, 2074
FaqI	GGGAC	5 cut(s)	92, 129, 806, 2069, 2296
FauNDI	CATATG	3 cut(s)	1077, 1262, 2146
FblI	GTMKAC	1 cut(s)	2028
Fnu4HI	GCNGC	7 cut(s)	492, 688, 691, 705, 830, 1549, 2013
FokI	GGATG	8 cut(s)	149, 940, 1094, 1211, 1222, 1835, 1845, 1919
FriOI	GRGCYC	2 cut(s)	40, 367
Fsp4HI	GCNGC	7 cut(s)	492, 688, 691, 705, 830, 1549, 2013
FspBI	CTAG	3 cut(s)	231, 1356, 2300
GlaI	GCGC	1 cut(s)	323
GluI	GCNGC	7 cut(s)	492, 688, 691, 705, 830, 1549, 2013
GsuI	CTGGAG	5 cut(s)	1086, 1626, 1780, 1833, 1935
HaeIII	GGCC	3 cut(s)	25, 462, 591
HapII	CCGG	2 cut(s)	212, 257
HhaI	GCGC	1 cut(s)	324
Hin6I	GCGC	1 cut(s)	322
HinP1I	GCGC	1 cut(s)	322
HincII	GTYRAC	2 cut(s)	1483, 2254
HindII	GTYRAC	2 cut(s)	1483, 2254
HindIII	AAGCTT	1 cut(s)	1854
HinfI	GANTC	9 cut(s)	341, 371, 470, 536, 602, 845, 941, 1528, 2284
HpaII	CCGG	2 cut(s)	212, 257
HphI	GGTGA	8 cut(s)	52, 545, 746, 797, 923, 1523, 1537, 2097
Hpy166II	GTNNAC	4 cut(s)	1483, 1514, 2029, 2254
Hpy188III	TCNNGA	6 cut(s)	134, 347, 869, 878, 1797, 1850
Hpy8I	GTNNAC	4 cut(s)	1483, 1514, 2029, 2254
HpyAV	CCTTC	8 cut(s)	134, 581, 866, 1049, 1724, 1823, 2077, 2329
HpyCH4III	ACNGT	4 cut(s)	1249, 1406, 1907, 2095
HpyCH4IV	ACGT	2 cut(s)	759, 1879
HpyF10VI	GCNNNNNNNGC	6 cut(s)	35, 328, 497, 518, 704, 776
HpyF3I	CTNAG	8 cut(s)	132, 169, 522, 825, 1269, 1776, 1944, 2202
HpySE526I	ACGT	2 cut(s)	759, 1879
HspAI	GCGC	1 cut(s)	322
KpnI	GGTACC	1 cut(s)	1000
Kzo9I	GATC	3 cut(s)	44, 1456, 1978
LmnI	GCTCC	5 cut(s)	362, 727, 748, 814, 1378
Lsp1109I	GCAGC	7 cut(s)	503, 677, 691, 699, 841, 1560, 1999
LweI	GCATC	8 cut(s)	487, 622, 658, 691, 877, 1328, 1876, 2081
MabI	ACCWGGT	1 cut(s)	2122
MaeI	CTAG	3 cut(s)	231, 1356, 2300
MaeII	ACGT	2 cut(s)	759, 1879
MaeIII	GTNAC	6 cut(s)	1069, 1084, 1688, 1843, 2103, 2357
MalI	GATC	3 cut(s)	46, 1458, 1980
MboI	GATC	3 cut(s)	44, 1456, 1978
MfeI	CAATTG	1 cut(s)	891
MflI	RGATCY	1 cut(s)	44
MhlI	GDGCHC	2 cut(s)	40, 367
MlyI	GAGTC	2 cut(s)	365, 2278
MmeI	TCCRAC	2 cut(s)	1608, 2006
Mph1103I	ATGCAT	3 cut(s)	1321, 1596, 1869
MroXI	GAANNNNTTC	1 cut(s)	1719
MseI	TTAA	4 cut(s)	543, 1097, 1131, 2166
MslI	CAYNNNNRTG	5 cut(s)	555, 615, 1010, 1080, 2110
MspA1I	CMGCKG	2 cut(s)	494, 690
MspCI	CTTAAG	1 cut(s)	2165
MspI	CCGG	2 cut(s)	212, 257
MspR9I	CCNGG	7 cut(s)	113, 368, 593, 684, 732, 1343, 2124
MunI	CAATTG	1 cut(s)	891
Mva1269I	GAATGC	3 cut(s)	324, 331, 1594
MvaI	CCWGG	7 cut(s)	113, 368, 593, 684, 732, 1343, 2124
MwoI	GCNNNNNNNGC	6 cut(s)	35, 328, 497, 518, 704, 776
NcoI	CCATGG	4 cut(s)	1005, 1374, 2111, 2291
NdeI	CATATG	3 cut(s)	1077, 1262, 2146
NdeII	GATC	3 cut(s)	44, 1456, 1978
NlaIV	GGNNCC	5 cut(s)	24, 364, 619, 729, 998
NmuCI	GTSAC	3 cut(s)	1688, 2103, 2357
NsiI	ATGCAT	3 cut(s)	1321, 1596, 1869
NspI	RCATGY	3 cut(s)	821, 1555, 1961
NspV	TTCGAA	2 cut(s)	335, 1684
OliI	CACNNNNGTG	1 cut(s)	615
PasI	CCCWGGG	1 cut(s)	112
PciI	ACATGT	1 cut(s)	1957
PcsI	WCGNNNNNNNCGW	1 cut(s)	2028
PctI	GAATGC	3 cut(s)	324, 331, 1594
PdmI	GAANNNNTTC	1 cut(s)	1719
PfeI	GAWTC	7 cut(s)	341, 470, 536, 602, 845, 941, 1528
PflMI	CCANNNNNTGG	2 cut(s)	1375, 2177
PfoI	TCCNGGA	1 cut(s)	1341
PkrI	GCNGC	7 cut(s)	493, 689, 692, 706, 831, 1550, 2014
PleI	GAGTC	2 cut(s)	365, 2278
PpsI	GAGTC	2 cut(s)	365, 2278
Ppu21I	YACGTR	1 cut(s)	760
PpuMI	RGGWCCY	1 cut(s)	2120
PscI	ACATGT	1 cut(s)	1957
PsiI	TTATAA	1 cut(s)	1898
Psp124BI	GAGCTC	1 cut(s)	40
Psp1406I	AACGTT	1 cut(s)	1879
Psp5II	RGGWCCY	1 cut(s)	2120
Psp6I	CCWGG	7 cut(s)	111, 366, 591, 682, 730, 1341, 2122
PspGI	CCWGG	7 cut(s)	111, 366, 591, 682, 730, 1341, 2122
PspN4I	GGNNCC	5 cut(s)	24, 364, 619, 729, 998
PspPI	GGNCC	4 cut(s)	23, 460, 1345, 2120
PspPPI	RGGWCCY	1 cut(s)	2120
PstI	CTGCAG	1 cut(s)	700
PstNI	CAGNNNCTG	1 cut(s)	704
PsuI	RGATCY	1 cut(s)	44
PvuII	CAGCTG	2 cut(s)	494, 690
RsaI	GTAC	2 cut(s)	675, 998
RsaNI	GTAC	2 cut(s)	674, 997
RseI	CAYNNNNRTG	5 cut(s)	555, 615, 1010, 1080, 2110
SacI	GAGCTC	1 cut(s)	40
SaqAI	TTAA	4 cut(s)	543, 1097, 1131, 2166
SatI	GCNGC	7 cut(s)	492, 688, 691, 705, 830, 1549, 2013
Sau3AI	GATC	3 cut(s)	44, 1456, 1978
Sau96I	GGNCC	4 cut(s)	23, 460, 1345, 2120
SchI	GAGTC	2 cut(s)	365, 2278
ScrFI	CCNGG	7 cut(s)	113, 368, 593, 684, 732, 1343, 2124
SduI	GDGCHC	2 cut(s)	40, 367
SexAI	ACCWGGT	1 cut(s)	2122
SfaNI	GCATC	8 cut(s)	487, 622, 658, 691, 877, 1328, 1876, 2081
SfcI	CTRYAG	1 cut(s)	696
SfuI	TTCGAA	2 cut(s)	335, 1684
SinI	GGWCC	2 cut(s)	1345, 2120
SmiMI	CAYNNNNRTG	5 cut(s)	555, 615, 1010, 1080, 2110
SmlI	CTYRAG	4 cut(s)	300, 345, 878, 2165
SmoI	CTYRAG	4 cut(s)	300, 345, 878, 2165
SspI	AATATT	2 cut(s)	973, 1769
SspMI	CTAG	3 cut(s)	231, 1356, 2300
SstI	GAGCTC	1 cut(s)	40
StyD4I	CCNGG	7 cut(s)	111, 366, 591, 682, 730, 1341, 2122
StyI	CCWWGG	4 cut(s)	1005, 1374, 2111, 2291
TaaI	ACNGT	4 cut(s)	1249, 1406, 1907, 2095
TaiI	ACGT	2 cut(s)	762, 1882
TaqI	TCGA	2 cut(s)	335, 1684
TatI	WGTACW	1 cut(s)	673
TfiI	GAWTC	7 cut(s)	341, 470, 536, 602, 845, 941, 1528
Tru1I	TTAA	4 cut(s)	543, 1097, 1131, 2166
Tru9I	TTAA	4 cut(s)	543, 1097, 1131, 2166
TscAI	CASTG	6 cut(s)	634, 1411, 1573, 1759, 2203, 2362
TseFI	GTSAC	3 cut(s)	1688, 2103, 2357
TseI	GCWGC	7 cut(s)	491, 687, 690, 704, 829, 1548, 2012
Tsp45I	GTSAC	3 cut(s)	1688, 2103, 2357
TspGWI	ACGGA	3 cut(s)	740, 1675, 2037
TspRI	CASTG	6 cut(s)	634, 1411, 1573, 1759, 2203, 2362
Van91I	CCANNNNNTGG	2 cut(s)	1375, 2177
Vha464I	CTTAAG	1 cut(s)	2165
VpaK11BI	GGWCC	2 cut(s)	1345, 2120
XapI	RAATTY	4 cut(s)	181, 1497, 1680, 1697
XceI	RCATGY	3 cut(s)	821, 1555, 1961
XcmI	CCANNNNNNNNNTGG	1 cut(s)	385
XmiI	GTMKAC	1 cut(s)	2028
XmnI	GAANNNNTTC	1 cut(s)	1719
XspI	CTAG	3 cut(s)	231, 1356, 2300
Zsp2I	ATGCAT	3 cut(s)	1321, 1596, 1869

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.