Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0014673

Physical Location & Seq

Genomic Coordinates Reverse (-)

3584120 .. 3589157

Gene Structure 5038 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00160630.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGGTTGAACCTGAAGACGAGGGGCCTGCTGTGAGAGCTCTCGGATCTCTGTTGAAGCTCACCCAAGTCTTTCTCTGGGACGATGGCACAAGAAACGACAGGTCGTGTTCCCTGGGACAGACTCAGCTCAGGATGAAGGAGACGATGGCTCAAACGCTCTTTCCTCAGGCTAGTGGATTCACTTTCATGCTTTCTTTGTATCTAGTAGTAAGTTTTAGTTGGAATTTCTGGGTTTCAGAATGTGGAACTTTGCCGGAGGATATGGAACTTACTAGACAGATGAATGAGTTGGGGCTTCCGGTCTCATTTCGCACAAATAAGGAGAAGCAGAAGAGAACAACTCAAGGAAAAAGAAAAGGAATGCGCCAGAAGCATTCAAACGAATCTCAAGAAGTTGTGGGTGGAGCCCTGGACGCTTCCAAAGTGAGTGTGGAGGAGATTGTATCTCCTATTATATTTGATGATAACCCACGCAGTTCTTTGTGTTGTATGTCAATGATGGGCCAAAGTGAATCATCTTCCTCTGATGTTGCAGCTGATGCCAATAATCCAGCAAGTTCAGCTAAGATTACTGGTGATTCTATTAAGGAACATAAGCGTGATGGAATAGTCGGCATTGTTTCTAATGAAGGCCAGGATTGTGATTCCTTACACAATGGTGCCATGCTCACTGATGCAACAACAATTTTAGCAAGTTCAACTGGGTTAGATGCTGTACTTTACCCAGGCAGCTGCTCTGCAGATGCTGCTAATGGCCATGATAAGACGGAGCCAGGTGAAAGACTTATGGAGCATGACCACGTAGAGTGTGCAATAATGGCTACCAGTGAAGCAGAAATAACCGAAAGTTGTGAGGAGCATGTCCCTGAGCAGCCATGTGTTTCTGAATCAGTCTCATGTTCCATATATCCAGAAGGTCTTGAGAATGATGCAATTGACAGCCAACAAAGTGGTGATTTTGGAGACTGGATGGTATATTGGGATTCTTACTACATGAGAAATTACTTCTATAATATTAGAACACATACTTCAACATGGTACCCACCCGTGGGCATGGAACATTTAGAAAATGTTGATAGCACATATAAGTCAAATGAAGGTGTTGCTGGAGTAACTCAGATGGATGTTACCACAGACTTAAAGGCAACAGATTTATGTGGTTTGAGTAAGTTTGACTCATTTGAAGAACCAATAAATGTTGATGTTTTACAATATCAACGACATGAACAGGTTTCAAGGGATGTTGAAATTACTGCTGCCAATACTTTATCGGATACATCCACTTCAACTGTTAGTGTAAGCATATGTCCTGAGCTTCCAGATGAACTTAATCAGAGCAATAACAGGTGCAATGATGGAAATGCATCATGCTTGTCATCAAATGTCCAGGACCACATTGCTAGTTGCAGAAACCAAACCATGGAGCAGGTTGCTGATGAGGTCTGCAACAGTGATTTGCAACCAATTTTTGTAGGAAAAAGTGCTGAACCAGATACTTCTGATCTTTATGATAAACCTAATGAAGTTTACTTTTGTGAGGAAATTCCTAAATATGGTGAACATGATGAGGCGATTCAAACATTAGATACGAGCAGCATGTCCAATTCATGCACTGAAGAAGTTGAGGATTGGAATATGCATTCTGAAACTGGAGTTCCTGCAACAAATGAGTTGGAAATGAAATCTGACCCTGCTTTACTAAAACAGAAAAAGAAAGCGAGAAGAATTCGAAGTCACAGAAAATTCTTCAACGAGAATGAAGAAGTTCTATTTGAAGGATTGCTGAAGAAGTGTTCCACTGATATTGGTAGATATTGGTCTCAGAGATATCTATTATTCTCCAGATATGATGATGGTATAAAAATGGATGAGGAAGGATGGTTTTCAGTTACTCCAGAAGCTCTAGCTATGCATCATGCCCAACGTTGTGGTAGTGGCATTATCGTTGACTGTTTTACTGGAGTTGGTGGTAATGCCATCCAGTTTGCTCAGATAAGCAAACATGTAACTGCCATTGATATTGATCCAACAAAGATTGATTATGCTCAACATAATGCTGCTATCTACGGAGTAGACGAAAGGATAGATTTCATAAAGGGGGACTTTTTCCATTTGGCATCAAGGTTGAAGGCAGATACAGTCTTTTTGTCACCACCATGGGGAGGACCTGGTTATGCAAGAGAAGAGACATATGACATTATGACAATGCTTAAGCCACATGATGGATATTTTCTCTTCAACACTGCTAAGCAAGTTGCTTCCAAGATTGTCATGTTTCTCCCAAGAAATGTTGATGTCAACCAATTAGCAGAGTTGTCTCTTTCAGGGACTCCACCATGGTCGCTAGAGGTGGAGAAAAACTTTGTAAATGGCAAGTTGAAGGGAATAACTGCTTACTTCAGTGACACGGCAAGGAGAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016741 GO:0032259 GO:0034641 GO:0036260 GO:0036261 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071164 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Methyltransf_15 WW

Protein Analysis

807

Amino Acids

89.81

Weight (kDa)

4.67

Isoelectric Point (pI)

42.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_15	PF09445	647 - 801	5.7e-43	RNA cap guanine-N2 methyltransferase
TRM5-TYW2_MTfase	PF02475	647 - 717	1.5e-07	TRM5/TYW2 methyltransferase domain
Methyltransf_31	PF13847	648 - 750	8e-07	Methyltransferase domain
Methyltransf_25	PF13649	648 - 715	6.8e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014673)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34310 FvH4_2g34310
malus_domestica	MD15G1081500.v1.1
prunus_persica	Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1
pyrus_communis	pycom15g07620 pycom15g07630
rosa_chinensis	RchiOBHm_Chr6g0308051
rosa_laevigata	RLG00000010672
rosa_multiflora	Rmu_sc0002624.1_g000003
rosa_roxburghii	Rroxscaffold_7G00160630
rosa_rugosa	Rorug06G0363400
rosa_samantha	Rh6AG472300 Rh6BG483400 Rh6DG474700
rosa_wichuraiana	Rw6G041220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1417
Acc65I	GGTACC	1 cut(s)	1038
AccB1I	GGYRCC	2 cut(s)	659, 1038
AccB7I	CCANNNNNTGG	2 cut(s)	1420, 2222
AccI	GTMKAC	1 cut(s)	2073
AclI	AACGTT	1 cut(s)	1924
AclWI	GGATC	2 cut(s)	52, 2018
AcoI	YGGCCR	1 cut(s)	754
AcsI	RAATTY	4 cut(s)	223, 1542, 1725, 1742
AcuI	CTGAAG	4 cut(s)	33, 1635, 1805, 2383
AfaI	GTAC	2 cut(s)	717, 1040
AfiI	CCNNNNNNNGG	7 cut(s)	1048, 1049, 1420, 1553, 2159, 2162, 2222
AflII	CTTAAG	1 cut(s)	2210
AflIII	ACRYGT	1 cut(s)	2002
AjnI	CCWGG	7 cut(s)	111, 408, 633, 724, 772, 1386, 2167
AleI	CACNNNNGTG	1 cut(s)	657
AluBI	AGCT	9 cut(s)	38, 58, 127, 536, 563, 732, 1315, 1901, 1907
AluI	AGCT	9 cut(s)	38, 58, 127, 536, 563, 732, 1315, 1901, 1907
Alw21I	GWGCWC	1 cut(s)	40
Alw26I	GTCTC	7 cut(s)	134, 307, 898, 957, 1824, 2180, 2322
AlwI	GGATC	2 cut(s)	52, 2018
AlwNI	CAGNNNCTG	1 cut(s)	746
AoxI	GGCC	4 cut(s)	23, 502, 631, 754
ApeKI	GCWGC	8 cut(s)	533, 729, 732, 746, 871, 1256, 1593, 2057
ApoI	RAATTY	4 cut(s)	223, 1542, 1725, 1742
Asp700I	GAANNNNTTC	1 cut(s)	1764
Asp718I	GGTACC	1 cut(s)	1038
AspLEI	GCGC	1 cut(s)	366
AspS9I	GGNCC	4 cut(s)	23, 502, 1390, 2165
AsuHPI	GGTGA	6 cut(s)	52, 587, 788, 965, 1568, 2142
AsuII	TTCGAA	1 cut(s)	1729
AvaII	GGWCC	2 cut(s)	1390, 2165
AxyI	CCTNAGG	1 cut(s)	165
BalI	TGGCCA	1 cut(s)	756
BanI	GGYRCC	2 cut(s)	659, 1038
BanII	GRGCYC	2 cut(s)	40, 409
BbsI	GAAGAC	1 cut(s)	21
Bbv12I	GWGCWC	1 cut(s)	40
BbvI	GCAGC	8 cut(s)	545, 719, 733, 741, 883, 1243, 1605, 2044
BcgI	CGANNNNNNTGC	2 cut(s)	8, 42
BciT130I	CCWGG	7 cut(s)	113, 410, 635, 726, 774, 1388, 2169
BciVI	GTATCC	1 cut(s)	1267
BcoDI	GTCTC	7 cut(s)	134, 307, 898, 957, 1824, 2180, 2322
BfaI	CTAG	6 cut(s)	171, 203, 273, 1401, 1904, 2345
BfmI	CTRYAG	1 cut(s)	738
BfrI	CTTAAG	1 cut(s)	2210
BfuAI	ACCTGC	1 cut(s)	1417
BfuI	GTATCC	1 cut(s)	1267
BisI	GCNGC	8 cut(s)	534, 730, 733, 747, 872, 1257, 1594, 2058
BlpI	GCTNAGC	1 cut(s)	2247
BlsI	GCNGC	8 cut(s)	535, 731, 734, 748, 873, 1258, 1595, 2059
Bme1390I	CCNGG	7 cut(s)	113, 410, 635, 726, 774, 1388, 2169
Bme18I	GGWCC	2 cut(s)	1390, 2165
BmgT120I	GGNCC	4 cut(s)	23, 502, 1390, 2165
BmiI	GGNNCC	5 cut(s)	24, 406, 661, 771, 1040
BmrFI	CCNGG	7 cut(s)	113, 410, 635, 726, 774, 1388, 2169
BmrI	ACTGGG	1 cut(s)	711
BmsI	GCATC	8 cut(s)	529, 664, 700, 733, 919, 1373, 1921, 2126
BmuI	ACTGGG	1 cut(s)	711
BpiI	GAAGAC	1 cut(s)	21
BplI	GAGNNNNNCTC	2 cut(s)	325, 357
BpmI	CTGGAG	5 cut(s)	1128, 1671, 1825, 1878, 1980
Bpu10I	CCTNAGC	3 cut(s)	128, 867, 1311
Bpu1102I	GCTNAGC	1 cut(s)	2247
Bpu14I	TTCGAA	1 cut(s)	1729
BpuEI	CTTGAG	3 cut(s)	327, 372, 941
BsaAI	YACGTR	1 cut(s)	802
BsaBI	GATNNNNATC	1 cut(s)	2022
BsaI	GGTCTC	2 cut(s)	307, 1824
BsaJI	CCNNGG	8 cut(s)	111, 112, 408, 724, 1047, 1419, 2156, 2336
BsaWI	WCCGGW	1 cut(s)	298
Bsc4I	CCNNNNNNNGG	7 cut(s)	1048, 1049, 1420, 1553, 2159, 2162, 2222
Bse1I	ACTGG	7 cut(s)	577, 706, 825, 971, 1654, 1963, 1981
Bse21I	CCTNAGG	1 cut(s)	165
Bse3DI	GCAATG	2 cut(s)	1357, 1395
Bse8I	GATNNNNATC	1 cut(s)	2022
BseBI	CCWGG	7 cut(s)	113, 410, 635, 726, 774, 1388, 2169
BseDI	CCNNGG	8 cut(s)	111, 112, 408, 724, 1047, 1419, 2156, 2336
BseGI	GGATG	8 cut(s)	138, 975, 1129, 1246, 1277, 1873, 1883, 1977
BseJI	GATNNNNATC	1 cut(s)	2022
BseLI	CCNNNNNNNGG	7 cut(s)	1048, 1049, 1420, 1553, 2159, 2162, 2222
BseMI	GCAATG	2 cut(s)	1357, 1395
BseMII	CTCAG	8 cut(s)	137, 142, 179, 858, 1130, 1302, 1835, 2003
BseNI	ACTGG	7 cut(s)	577, 706, 825, 971, 1654, 1963, 1981
BseRI	GAGGAG	2 cut(s)	449, 869
BseXI	GCAGC	8 cut(s)	545, 719, 733, 741, 883, 1243, 1605, 2044
BshFI	GGCC	4 cut(s)	25, 504, 633, 756
BshNI	GGYRCC	2 cut(s)	659, 1038
BsiHKAI	GWGCWC	1 cut(s)	40
BsiSI	CCGG	2 cut(s)	254, 299
BslFI	GGGAC	5 cut(s)	92, 129, 848, 2114, 2341
BslI	CCNNNNNNNGG	7 cut(s)	1048, 1049, 1420, 1553, 2159, 2162, 2222
BsmAI	GTCTC	7 cut(s)	134, 307, 898, 957, 1824, 2180, 2322
BsmBI	CGTCTC	1 cut(s)	134
BsmFI	GGGAC	5 cut(s)	92, 129, 848, 2114, 2341
BsmI	GAATGC	3 cut(s)	366, 373, 1639
BsnI	GGCC	4 cut(s)	25, 504, 633, 756
Bso31I	GGTCTC	2 cut(s)	307, 1824
Bsp119I	TTCGAA	1 cut(s)	1729
Bsp1286I	GDGCHC	2 cut(s)	40, 409
Bsp143I	GATC	3 cut(s)	44, 1501, 2023
Bsp1720I	GCTNAGC	1 cut(s)	2247
Bsp19I	CCATGG	3 cut(s)	1419, 2156, 2336
BspANI	GGCC	4 cut(s)	25, 504, 633, 756
BspCNI	CTCAG	8 cut(s)	136, 141, 178, 859, 1129, 1303, 1834, 2002
BspLI	GGNNCC	5 cut(s)	24, 406, 661, 771, 1040
BspMAI	CTGCAG	1 cut(s)	742
BspMI	ACCTGC	1 cut(s)	1417
BspPI	GGATC	2 cut(s)	52, 2018
BspT104I	TTCGAA	1 cut(s)	1729
BspT107I	GGYRCC	2 cut(s)	659, 1038
BspTI	CTTAAG	1 cut(s)	2210
BspTNI	GGTCTC	2 cut(s)	307, 1824
BsrDI	GCAATG	2 cut(s)	1357, 1395
BsrI	ACTGG	7 cut(s)	577, 706, 825, 971, 1654, 1963, 1981
BssECI	CCNNGG	8 cut(s)	111, 112, 408, 724, 1047, 1419, 2156, 2336
BssMI	GATC	3 cut(s)	44, 1501, 2023
BssT1I	CCWWGG	3 cut(s)	1419, 2156, 2336
Bst2UI	CCWGG	7 cut(s)	113, 410, 635, 726, 774, 1388, 2169
Bst4CI	ACNGT	4 cut(s)	1291, 1451, 1952, 2140
Bst6I	CTCTTC	3 cut(s)	326, 2178, 2240
BstAFI	CTTAAG	1 cut(s)	2210
BstAPI	GCANNNNNTGC	2 cut(s)	539, 746
BstBAI	YACGTR	1 cut(s)	802
BstBI	TTCGAA	1 cut(s)	1729
BstC8I	GCNNGC	1 cut(s)	27
BstDSI	CCRYGG	4 cut(s)	1047, 1419, 2156, 2336
BstF5I	GGATG	8 cut(s)	138, 975, 1129, 1246, 1277, 1873, 1883, 1977
BstHHI	GCGC	1 cut(s)	366
BstKTI	GATC	3 cut(s)	47, 1504, 2026
BstMAI	GTCTC	7 cut(s)	134, 307, 898, 957, 1824, 2180, 2322
BstMBI	GATC	3 cut(s)	44, 1501, 2023
BstMWI	GCNNNNNNNGC	7 cut(s)	35, 370, 413, 539, 560, 746, 818
BstNI	CCWGG	7 cut(s)	113, 410, 635, 726, 774, 1388, 2169
BstNSI	RCATGY	3 cut(s)	863, 1600, 2006
BstSCI	CCNGG	7 cut(s)	111, 408, 633, 724, 772, 1386, 2167
BstSFI	CTRYAG	1 cut(s)	738
BstV1I	GCAGC	8 cut(s)	545, 719, 733, 741, 883, 1243, 1605, 2044
BstV2I	GAAGAC	1 cut(s)	21
BstX2I	RGATCY	1 cut(s)	44
BstXI	CCANNNNNNTGG	2 cut(s)	950, 1928
BstYI	RGATCY	1 cut(s)	44
Bsu36I	CCTNAGG	1 cut(s)	165
BsuI	GTATCC	1 cut(s)	1267
BsuRI	GGCC	4 cut(s)	25, 504, 633, 756
BtgI	CCRYGG	4 cut(s)	1047, 1419, 2156, 2336
BtsCI	GGATG	8 cut(s)	138, 975, 1129, 1246, 1277, 1873, 1883, 1977
BtsI	GCAGTG	1 cut(s)	2241
BtsIMutI	CAGTG	7 cut(s)	669, 832, 1456, 1611, 1797, 2241, 2407
BveI	ACCTGC	1 cut(s)	1417
Cac8I	GCNNGC	1 cut(s)	27
CaiI	CAGNNNCTG	1 cut(s)	746
CfoI	GCGC	1 cut(s)	366
Cfr13I	GGNCC	4 cut(s)	23, 502, 1390, 2165
CseI	GACGC	1 cut(s)	422
CsiI	ACCWGGT	1 cut(s)	2167
Csp6I	GTAC	2 cut(s)	716, 1039
CspCI	CAANNNNNGTGG	2 cut(s)	1786, 1821
CviQI	GTAC	2 cut(s)	716, 1039
DpnI	GATC	3 cut(s)	46, 1503, 2025
DpnII	GATC	3 cut(s)	44, 1501, 2023
EaeI	YGGCCR	1 cut(s)	754
Eam1104I	CTCTTC	3 cut(s)	326, 2178, 2240
EarI	CTCTTC	3 cut(s)	326, 2178, 2240
Ecl136II	GAGCTC	1 cut(s)	38
Eco130I	CCWWGG	3 cut(s)	1419, 2156, 2336
Eco24I	GRGCYC	2 cut(s)	40, 409
Eco31I	GGTCTC	2 cut(s)	307, 1824
Eco32I	GATATC	1 cut(s)	1829
Eco47I	GGWCC	2 cut(s)	1390, 2165
Eco53kI	GAGCTC	1 cut(s)	38
Eco57I	CTGAAG	4 cut(s)	33, 1635, 1805, 2383
Eco81I	CCTNAGG	1 cut(s)	165
EcoICRI	GAGCTC	1 cut(s)	38
EcoO109I	RGGNCCY	2 cut(s)	23, 2165
EcoRI	GAATTC	1 cut(s)	1725
EcoRII	CCWGG	7 cut(s)	111, 408, 633, 724, 772, 1386, 2167
EcoRV	GATATC	1 cut(s)	1829
EcoT14I	CCWWGG	3 cut(s)	1419, 2156, 2336
EcoT22I	ATGCAT	3 cut(s)	1366, 1641, 1914
EcoT38I	GRGCYC	2 cut(s)	40, 409
ErhI	CCWWGG	3 cut(s)	1419, 2156, 2336
Esp3I	CGTCTC	1 cut(s)	134
FalI	AAGNNNNNCTT	2 cut(s)	2087, 2119
FaqI	GGGAC	5 cut(s)	92, 129, 848, 2114, 2341
FauNDI	CATATG	2 cut(s)	1304, 2191
FblI	GTMKAC	1 cut(s)	2073
Fnu4HI	GCNGC	8 cut(s)	534, 730, 733, 747, 872, 1257, 1594, 2058
FokI	GGATG	8 cut(s)	145, 982, 1136, 1253, 1264, 1880, 1890, 1964
FriOI	GRGCYC	2 cut(s)	40, 409
Fsp4HI	GCNGC	8 cut(s)	534, 730, 733, 747, 872, 1257, 1594, 2058
FspBI	CTAG	6 cut(s)	171, 203, 273, 1401, 1904, 2345
GlaI	GCGC	1 cut(s)	365
GluI	GCNGC	8 cut(s)	534, 730, 733, 747, 872, 1257, 1594, 2058
GsuI	CTGGAG	5 cut(s)	1128, 1671, 1825, 1878, 1980
HaeIII	GGCC	4 cut(s)	25, 504, 633, 756
HapII	CCGG	2 cut(s)	254, 299
HgaI	GACGC	1 cut(s)	422
HhaI	GCGC	1 cut(s)	366
Hin6I	GCGC	1 cut(s)	364
HinP1I	GCGC	1 cut(s)	364
HincII	GTYRAC	2 cut(s)	1948, 2299
HindII	GTYRAC	2 cut(s)	1948, 2299
HpaII	CCGG	2 cut(s)	254, 299
HphI	GGTGA	6 cut(s)	52, 587, 788, 965, 1568, 2142
Hpy166II	GTNNAC	5 cut(s)	1528, 1559, 1948, 2074, 2299
Hpy188III	TCNNGA	8 cut(s)	130, 389, 911, 920, 1310, 1319, 1842, 1895
Hpy8I	GTNNAC	5 cut(s)	1528, 1559, 1948, 2074, 2299
HpyAV	CCTTC	8 cut(s)	130, 623, 908, 1091, 1769, 1868, 2122, 2374
HpyCH4III	ACNGT	4 cut(s)	1291, 1451, 1952, 2140
HpyCH4IV	ACGT	2 cut(s)	801, 1924
HpyF10VI	GCNNNNNNNGC	7 cut(s)	35, 370, 413, 539, 560, 746, 818
HpySE526I	ACGT	2 cut(s)	801, 1924
HspAI	GCGC	1 cut(s)	364
KpnI	GGTACC	1 cut(s)	1042
Kzo9I	GATC	3 cut(s)	44, 1501, 2023
LmnI	GCTCC	5 cut(s)	404, 769, 790, 856, 1423
Lsp1109I	GCAGC	8 cut(s)	545, 719, 733, 741, 883, 1243, 1605, 2044
LweI	GCATC	8 cut(s)	529, 664, 700, 733, 919, 1373, 1921, 2126
MabI	ACCWGGT	1 cut(s)	2167
MaeI	CTAG	6 cut(s)	171, 203, 273, 1401, 1904, 2345
MaeII	ACGT	2 cut(s)	801, 1924
MaeIII	GTNAC	7 cut(s)	1111, 1126, 1733, 1888, 2005, 2148, 2402
MalI	GATC	3 cut(s)	46, 1503, 2025
MboI	GATC	3 cut(s)	44, 1501, 2023
MfeI	CAATTG	1 cut(s)	933
MflI	RGATCY	1 cut(s)	44
MhlI	GDGCHC	2 cut(s)	40, 409
MlsI	TGGCCA	1 cut(s)	756
MluNI	TGGCCA	1 cut(s)	756
MlyI	GAGTC	3 cut(s)	115, 1169, 2323
MmeI	TCCRAC	3 cut(s)	200, 1653, 2051
Mox20I	TGGCCA	1 cut(s)	756
Mph1103I	ATGCAT	3 cut(s)	1366, 1641, 1914
MroXI	GAANNNNTTC	1 cut(s)	1764
MscI	TGGCCA	1 cut(s)	756
MseI	TTAA	4 cut(s)	585, 1139, 1329, 2211
MslI	CAYNNNNRTG	4 cut(s)	185, 597, 657, 2155
Msp20I	TGGCCA	1 cut(s)	756
MspA1I	CMGCKG	2 cut(s)	536, 732
MspCI	CTTAAG	1 cut(s)	2210
MspI	CCGG	2 cut(s)	254, 299
MspR9I	CCNGG	7 cut(s)	113, 410, 635, 726, 774, 1388, 2169
MunI	CAATTG	1 cut(s)	933
Mva1269I	GAATGC	3 cut(s)	366, 373, 1639
MvaI	CCWGG	7 cut(s)	113, 410, 635, 726, 774, 1388, 2169
MwoI	GCNNNNNNNGC	7 cut(s)	35, 370, 413, 539, 560, 746, 818
NcoI	CCATGG	3 cut(s)	1419, 2156, 2336
NdeI	CATATG	2 cut(s)	1304, 2191
NdeII	GATC	3 cut(s)	44, 1501, 2023
NlaIV	GGNNCC	5 cut(s)	24, 406, 661, 771, 1040
NmuCI	GTSAC	3 cut(s)	1733, 2148, 2402
NsiI	ATGCAT	3 cut(s)	1366, 1641, 1914
NspI	RCATGY	3 cut(s)	863, 1600, 2006
NspV	TTCGAA	1 cut(s)	1729
OliI	CACNNNNGTG	1 cut(s)	657
PasI	CCCWGGG	1 cut(s)	112
PciI	ACATGT	1 cut(s)	2002
PcsI	WCGNNNNNNNCGW	1 cut(s)	2073
PctI	GAATGC	3 cut(s)	366, 373, 1639
PdmI	GAANNNNTTC	1 cut(s)	1764
PfeI	GAWTC	8 cut(s)	177, 383, 512, 578, 644, 887, 983, 1573
PflMI	CCANNNNNTGG	2 cut(s)	1420, 2222
PfoI	TCCNGGA	1 cut(s)	1386
PkrI	GCNGC	8 cut(s)	535, 731, 734, 748, 873, 1258, 1595, 2059
PleI	GAGTC	3 cut(s)	115, 1169, 2323
PpsI	GAGTC	3 cut(s)	115, 1169, 2323
Ppu21I	YACGTR	1 cut(s)	802
PpuMI	RGGWCCY	1 cut(s)	2165
PscI	ACATGT	1 cut(s)	2002
Psp124BI	GAGCTC	1 cut(s)	40
Psp1406I	AACGTT	1 cut(s)	1924
Psp5II	RGGWCCY	1 cut(s)	2165
Psp6I	CCWGG	7 cut(s)	111, 408, 633, 724, 772, 1386, 2167
PspGI	CCWGG	7 cut(s)	111, 408, 633, 724, 772, 1386, 2167
PspN4I	GGNNCC	5 cut(s)	24, 406, 661, 771, 1040
PspPI	GGNCC	4 cut(s)	23, 502, 1390, 2165
PspPPI	RGGWCCY	1 cut(s)	2165
PstI	CTGCAG	1 cut(s)	742
PstNI	CAGNNNCTG	1 cut(s)	746
PsuI	RGATCY	1 cut(s)	44
PvuII	CAGCTG	2 cut(s)	536, 732
RsaI	GTAC	2 cut(s)	717, 1040
RsaNI	GTAC	2 cut(s)	716, 1039
RseI	CAYNNNNRTG	4 cut(s)	185, 597, 657, 2155
SacI	GAGCTC	1 cut(s)	40
SaqAI	TTAA	4 cut(s)	585, 1139, 1329, 2211
SatI	GCNGC	8 cut(s)	534, 730, 733, 747, 872, 1257, 1594, 2058
Sau3AI	GATC	3 cut(s)	44, 1501, 2023
Sau96I	GGNCC	4 cut(s)	23, 502, 1390, 2165
SchI	GAGTC	3 cut(s)	115, 1169, 2323
ScrFI	CCNGG	7 cut(s)	113, 410, 635, 726, 774, 1388, 2169
SduI	GDGCHC	2 cut(s)	40, 409
SexAI	ACCWGGT	1 cut(s)	2167
SfaNI	GCATC	8 cut(s)	529, 664, 700, 733, 919, 1373, 1921, 2126
SfcI	CTRYAG	1 cut(s)	738
SfuI	TTCGAA	1 cut(s)	1729
SinI	GGWCC	2 cut(s)	1390, 2165
SmiMI	CAYNNNNRTG	4 cut(s)	185, 597, 657, 2155
SmlI	CTYRAG	4 cut(s)	342, 387, 920, 2210
SmoI	CTYRAG	4 cut(s)	342, 387, 920, 2210
SspI	AATATT	1 cut(s)	1015
SspMI	CTAG	6 cut(s)	171, 203, 273, 1401, 1904, 2345
SstI	GAGCTC	1 cut(s)	40
StyD4I	CCNGG	7 cut(s)	111, 408, 633, 724, 772, 1386, 2167
StyI	CCWWGG	3 cut(s)	1419, 2156, 2336
TaaI	ACNGT	4 cut(s)	1291, 1451, 1952, 2140
TaiI	ACGT	2 cut(s)	804, 1927
TaqI	TCGA	1 cut(s)	1729
TatI	WGTACW	1 cut(s)	715
TfiI	GAWTC	8 cut(s)	177, 383, 512, 578, 644, 887, 983, 1573
Tru1I	TTAA	4 cut(s)	585, 1139, 1329, 2211
Tru9I	TTAA	4 cut(s)	585, 1139, 1329, 2211
TscAI	CASTG	7 cut(s)	676, 832, 1456, 1618, 1804, 2248, 2407
TseFI	GTSAC	3 cut(s)	1733, 2148, 2402
TseI	GCWGC	8 cut(s)	533, 729, 732, 746, 871, 1256, 1593, 2057
Tsp45I	GTSAC	3 cut(s)	1733, 2148, 2402
TspGWI	ACGGA	2 cut(s)	782, 2082
TspRI	CASTG	7 cut(s)	676, 832, 1456, 1618, 1804, 2248, 2407
Van91I	CCANNNNNTGG	2 cut(s)	1420, 2222
Vha464I	CTTAAG	1 cut(s)	2210
VpaK11BI	GGWCC	2 cut(s)	1390, 2165
XapI	RAATTY	4 cut(s)	223, 1542, 1725, 1742
XceI	RCATGY	3 cut(s)	863, 1600, 2006
XcmI	CCANNNNNNNNNTGG	1 cut(s)	427
XmiI	GTMKAC	1 cut(s)	2073
XmnI	GAANNNNTTC	1 cut(s)	1764
XspI	CTAG	6 cut(s)	171, 203, 273, 1401, 1904, 2345
Zsp2I	ATGCAT	3 cut(s)	1366, 1641, 1914

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.