Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0014673

Physical Location & Seq

Genomic Coordinates Forward (+)

64036950 .. 64046618

Gene Structure 9669 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG474700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3939 bp

ATGGTTGAAGCTGAAGACGAGGGGCCTGCTGTGAGAGCTCTCGGATCTCTGTTGAAGCTCACCCAAGTCTTTCTCTGGGACGATGGCACAAGAAACGACAGGTCGTTTTCCCTGGGACAGACTAAACATGCTCAGGATGAAGGAGACGATGGCTCAAACGCTCTTTCCTCAGAATGTGGAACTTTGCCGGAGGATATGGAACTTACTAGACAGATGAATGAGTTGGGGCTTCCGGTCTCATTTCGCACAAATAAGGAGAAGCAGAAGAGAACAACTCAAGGAAAAAGAAAAGGAATGCGCCAGAAGGATTCGAACGAATCTCAAGAAGTTGTGGGTGGAGCCCTGGACTCTTCCAAAGTGAGTGTGGAGGAGATTGTAACTCCTATTATATTTGATGATAACCCAAGCAGTTCTTTGTGTTGTATGTCAATGATGGGCCAAAGTGAATCATCTTCCTCTGATGTTGCAGCTGATGCCAATAATCCAGCAAGTTCAGCTAAGATTACTGGTGATTCTATTAAGGAACATAAGCGTGATGGAATAGTCGGCATTGTTTCTAATGAAGGCCAGGATTGTGATTCCTTACACAATGGTGCCACGCTCACTGATGCAACAACAATTTTAGCAAGTTCAACTGGGTTAGATGCTGTACTTTACCCAGGCAGCTGCTCTGCAGATGCTGCTAATGGCCATGATAAGACGGAGCCAGGTGAAAGACTTATGGAGCATGACCACGTAGAGTGTGCAATAATGGCTACCAGTGAAGCAGAAATAACCGAAAGTTGTGAGGAGCATGTCCCTGAGCAGCCATGTGTTTCTGAATCAGTCTCATGTTCCATATATCCAGAAGGTCTTGAGAATGATGCAATTGACAGCCAACAAAGTGGTGATTTTGGAGACTGGATGGTATATTGGGATTCTTACTACATGAGAAATTACTTCTATAATATTAGAACACATACTTCAACATGGTACCCACCTGTGGGCATGGAACATTTAGAAAATGTTGATAGCACATATAAGTCAAATGAAGGTGTTGCTGGAGTAACTCAGATGGATGTTACCACAGACTTAAAGGCAACAGATTTATGTGGTTTGAGTAAGTTTGACTCATTTGAAGAACCAATAAATGTTGATGTTTTACAATATCAACGATATGAAGAGGTTTCAAGGGATGTTGAAATTACTGCTGCCAATACTTTATCGGATACATCCACTTCAACTGTCAGTGTAAGCATATGTCCTGAGCTTCCAGATGAACTTAATCAGAGCAATAACAGGTGCAATGATGGAAATGCATCATGCTTGTCATCAAATGTCCAGGACCACATTGCTAGTTGCAGAAACCAAACCATGGAGCAGGTTGCTGATGACGTCTGCAACAGTGATTTGCAACCAATTTTTGCAGGAAAAAGTGCTGAACCAGATACTTCTGATCTCTATGATAAACCTAATGAAGTTTACTTTTGTGAGGAAATTCCTAAATATGGTGAACATGATGAGGCGATTCAAACATTAGATACAAGCAGCATGTCCAATTCATGCACTGAAGAAGTTGAGGATTGGAATATGCATTCTGAAACTGGAGCTCCTGCAACAAATGAGTTGGAAATGAAATCTGACCCTGCTTTAGTAAAACGGAAAAAGAAAGTGAGAAGAATTCGAAGTCACAGAAAATTCTTCAATGAGAATGAAGAAGTTCTATTTGAAGGATTGCTGGAGAAGTGTTCCACTGATATTGGTAAATATTGGTCTCAGAGATATCTATTATTCTCCAGATATGATGATGGTATAAAAATGGATGAGGAAGGATGGTTTTCAGTTACTCCAGAAGCTCTAGCTATGCATCATGCCCAACGTTGTGGTAGTGGCATTATCATTGACTGTTTTACTGGAGTTGGTGGTAATGCCATCCAATTTGCTCAGATAAGCAAACATGTAACTGCCATTGATATTGATCCAACAAAGATTGATTATGCTCAACATAATGCTGCTATCTACGGAGTAGACGAAAGGATAGATTTCATAAAGGGGGACTTTTTCCATTTGGCATCAAGGTTGAAGGCAGATACAGTCTTTTTGTCACCACCATGGGGAGGACCTGGTTATGCAAGAGAAGAGACATATGACATTATGACAATGCTTAAGCCACATGATGGATATTTTCTCTTCAACACTGCTAAGCAAGTTGCTTCCAAGATTGTCATGTTTCTCCCAAGAAATGTTGATGTCAACCAATTAGCAGAGTTGTCTCTTTCAGGGACCCCACCATGGTCGCTAGAGAACTCGGCGGCGCACCGTCACCAGCTTGAAGAGAGCTCCAGTGGTCCGGATCGCGATCCAGATCAGAAGACGAAGCCCGCTTTACACATCACCAGCCACCACCAGGGTCGTCGATCCAGAAAAGGAGAAAAGGCCAAAGGTACTACAGCTAAGCCTTCTGGACGACGTCGCTTTCTTCCTTTCCTTTTCAGTTTTTCCAAACGATGTGACTTTCTGTTTTCCCTGACTTTCACTCTTTGGCTTGGAGCTTGCACAGTTCAAAAGCAAGACAACGACGCCGACTTGGTTCTTGATTCAGCACGAAGCTCTTTCTCTCTCGCCTTGAAAGAATGCCAGGACCGTCGATCTAGATCTGAAGCTCTGTCCAAGAAGCTAGACCGGCCGAGACCCGCTTCTTTGGATTTGAACAATGTCACCGCCTCGTCTCCGAGATTGGGGACCTTAAAGAAGAGCTCAATGGCGTCTCGCAAGTCTGGAACCTTCCCAAGTCCAGGAACACCTACTTACCGGCATACTAGTCTTGGAGTGCACAAGGGTTGGAGCTCGGAGCGAGTTCCGGCCAATGCTAATTTGGGGGGCAGGAAGAATACAACTGCTGCTGCTATGTTGCCATTCAACAATGGGAGGACGTTGCCGTCGAAATGGGAAGATGCAGAGAGGTGGATTTTCAGTCCGGTTCAGGGGGATGGTGTGACGAGGCAGTCGAATCAGCAGGCCCAGAGGAGGCCCAAGGCAAAGAGTGGTCCGCTCGGCCCTCCTGGAGTTGCATATTATTCATTGTATTCACCAGCAATGCCGATGTTTGATGGAGGTAGCGTTCCTAATTTCATGGCTGGTTCTCCGTTTTCAGCTGGGGTTATGGCAGCGGATGGAATGGCATTCCACTCCTCCGGTGCTCATAATGGAGCCTTTTCCAATAGGGCTGAGCCTTTCATTGCTCGTTCGGTTAGCGTGCATGGTTGCTCTGAGGTGTTGGACCAGCAGTCATCCCTGCCTGAGTCTCAAGATGAAAAGCTTGATGGGGTTAAGGATGCAGCCACCAATGTTTCTCGTGCAGTTTCACGAAGGGACATGGCAACCCAAATGAGCCCTGATGACAGCATTCACTCGTCTCCCAAGGAGGGACATGATTACTCTGCTTCTACGTCCTCTTTCTTCCCTATTGAGGAAATGCAGAGCGCCCCTTCTTTGAAATTGGAAGTCAGGGATGTGCAGGTAGATGAACGGGTCACTGTGACAAGGTGGTCCAAGAAACATAAAGCCCGAATACCTGGGAAGGGCTCTGATATTGTGGATAGATGGAAAAAGAAAGATGCTGATGTTCGATCTTGTGCTTGGGATCTCTCTGATTCAGCAAAAAACGTTTCAAAAGTTAAAAGAGAGGAAGCTAAAATCGCTGCATGGGAGAATCTGCAGAAGGCAAAAGCTGAGGCAGCAATACGGAAACTAGAGATGAAGCTGGAAAAGAAGAGGTCATCATCCATGGATAAAATTATGAACAAGTTGAGATCAGCTCAGAAAAAAGCTCAAGACATGAGAAGCATAGTGTCAGACAATCAGGCACATGATGGAAGGAGCTCACATAAAACTTTATCAATTCGTAGGACTCGTCATATGGGTTCTTTTAGTGGTTGCTTTACGTGTCATGCTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016741 GO:0032259 GO:0034641 GO:0036260 GO:0036261 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071164 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Methyltransf_15 WW

Protein Analysis

1312

Amino Acids

144.81

Weight (kDa)

5.6

Isoelectric Point (pI)

53.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_15	PF09445	625 - 751	1e-40	RNA cap guanine-N2 methyltransferase
TRM5-TYW2_MTfase	PF02475	625 - 695	5.7e-07	TRM5/TYW2 methyltransferase domain
Methyltransf_31	PF13847	626 - 728	5.2e-07	Methyltransferase domain
Remorin_C	PF03763	1202 - 1300	6.9e-24	Remorin, C-terminal region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014673)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34310 FvH4_2g34310
malus_domestica	MD15G1081500.v1.1
prunus_persica	Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1
pyrus_communis	pycom15g07620 pycom15g07630
rosa_chinensis	RchiOBHm_Chr6g0308051
rosa_laevigata	RLG00000010672
rosa_multiflora	Rmu_sc0002624.1_g000003
rosa_roxburghii	Rroxscaffold_7G00160630
rosa_rugosa	Rorug06G0363400
rosa_samantha	Rh6AG472300 Rh6BG483400 Rh6DG474700
rosa_wichuraiana	Rw6G041220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	2917, 2983, 3526
AatII	GACGTC	2 cut(s)	1377, 2452
Acc36I	ACCTGC	2 cut(s)	1351, 3487
Acc65I	GGTACC	1 cut(s)	972
AccB1I	GGYRCC	2 cut(s)	593, 972
AccB7I	CCANNNNNTGG	2 cut(s)	1354, 2156
AccBSI	CCGCTC	1 cut(s)	3031
AccI	GTMKAC	1 cut(s)	2007
AccII	CGCG	1 cut(s)	2337
AccIII	TCCGGA	1 cut(s)	2329
AciI	CCGC	6 cut(s)	2291, 2361, 2673, 2700, 3029, 3149
AclI	AACGTT	2 cut(s)	1858, 3645
AclWI	GGATC	6 cut(s)	52, 1952, 2333, 2340, 2391, 3630
AcoI	YGGCCR	3 cut(s)	688, 2663, 2841
AcsI	RAATTY	3 cut(s)	1476, 1659, 1676
AcuI	CTGAAG	3 cut(s)	33, 1569, 2658
AcyI	GRCGYC	4 cut(s)	1374, 2449, 2559, 2744
AfaI	GTAC	3 cut(s)	651, 974, 2425
AflII	CTTAAG	1 cut(s)	2144
AflIII	ACRYGT	2 cut(s)	1936, 3923
AhdI	GACNNNNNGTC	1 cut(s)	3265
AhlI	ACTAGT	1 cut(s)	2798
AleI	CACNNNNGTG	1 cut(s)	591
AloI	GAACNNNNNNTCC	2 cut(s)	3084, 3116
Alw21I	GWGCWC	8 cut(s)	40, 1591, 2321, 2738, 2814, 2828, 3181, 3863
Alw44I	GTGCAC	1 cut(s)	2810
AlwI	GGATC	6 cut(s)	52, 1952, 2333, 2340, 2391, 3630
AlwNI	CAGNNNCTG	1 cut(s)	680
Aor13HI	TCCGGA	1 cut(s)	2329
ApaLI	GTGCAC	1 cut(s)	2810
ApoI	RAATTY	3 cut(s)	1476, 1659, 1676
Asp700I	GAANNNNTTC	1 cut(s)	1698
Asp718I	GGTACC	1 cut(s)	972
AspLEI	GCGC	3 cut(s)	300, 2296, 3464
AsuII	TTCGAA	2 cut(s)	311, 1663
AvaII	GGWCC	9 cut(s)	1324, 2099, 2262, 2327, 2620, 2721, 3026, 3259, 3528
BaeGI	GKGCMC	1 cut(s)	2814
BalI	TGGCCA	1 cut(s)	690
BanI	GGYRCC	2 cut(s)	593, 972
BanII	GRGCYC	9 cut(s)	40, 343, 1591, 2321, 2738, 2828, 3374, 3566, 3863
BauI	CACGAG	1 cut(s)	3333
BbsI	GAAGAC	2 cut(s)	21, 2357
Bbv12I	GWGCWC	8 cut(s)	40, 1591, 2321, 2738, 2814, 2828, 3181, 3863
BbvCI	CCTCAGC	1 cut(s)	3711
BceAI	ACGGC	1 cut(s)	2902
BcgI	CGANNNNNNTGC	2 cut(s)	8, 42
BciVI	GTATCC	1 cut(s)	1201
BcuI	ACTAGT	1 cut(s)	2798
BfaI	CTAG	8 cut(s)	207, 1335, 1838, 2279, 2631, 2657, 2799, 3731
BfmI	CTRYAG	3 cut(s)	672, 2427, 3695
BfoI	RGCGCY	1 cut(s)	3465
BfrI	CTTAAG	1 cut(s)	2144
BfuAI	ACCTGC	2 cut(s)	1351, 3487
BfuI	GTATCC	1 cut(s)	1201
BglII	AGATCT	1 cut(s)	2633
BlpI	GCTNAGC	3 cut(s)	2181, 2433, 3207
Bme18I	GGWCC	9 cut(s)	1324, 2099, 2262, 2327, 2620, 2721, 3026, 3259, 3528
BmeRI	GACNNNNNGTC	1 cut(s)	3265
BmrI	ACTGGG	1 cut(s)	645
BmuI	ACTGGG	1 cut(s)	645
BpiI	GAAGAC	2 cut(s)	21, 2357
BplI	GAGNNNNNCTC	4 cut(s)	259, 291, 3781, 3813
BpmI	CTGGAG	8 cut(s)	1062, 1605, 1739, 1759, 1812, 1914, 2305, 3063
Bpu10I	CCTNAGC	4 cut(s)	132, 801, 1245, 3711
Bpu1102I	GCTNAGC	3 cut(s)	2181, 2433, 3207
Bpu14I	TTCGAA	2 cut(s)	311, 1663
BpuEI	CTTGAG	5 cut(s)	261, 306, 875, 3270, 3795
BsaAI	YACGTR	2 cut(s)	736, 3924
BsaBI	GATNNNNATC	4 cut(s)	1956, 2337, 2343, 2632
BsaHI	GRCGYC	4 cut(s)	1374, 2449, 2559, 2744
BsaI	GGTCTC	3 cut(s)	241, 1758, 2662
BsaWI	WCCGGW	4 cut(s)	232, 2329, 2956, 3173
BsaXI	ACNNNNNCTCC	4 cut(s)	1573, 1603, 3084, 3114
Bse118I	RCCGGY	2 cut(s)	2661, 2790
Bse1I	ACTGG	7 cut(s)	511, 640, 759, 905, 1588, 1897, 2322
Bse3DI	GCAATG	4 cut(s)	1291, 1329, 3081, 3216
Bse8I	GATNNNNATC	4 cut(s)	1956, 2337, 2343, 2632
BseAI	TCCGGA	1 cut(s)	2329
BseJI	GATNNNNATC	4 cut(s)	1956, 2337, 2343, 2632
BseMI	GCAATG	4 cut(s)	1291, 1329, 3081, 3216
BseNI	ACTGG	7 cut(s)	511, 640, 759, 905, 1588, 1897, 2322
BseRI	GAGGAG	4 cut(s)	383, 803, 3019, 3160
BseSI	GKGCMC	1 cut(s)	2814
BseX3I	CGGCCG	1 cut(s)	2663
BseYI	CCCAGC	1 cut(s)	3134
BsgI	GTGCAG	2 cut(s)	3357, 3515
Bsh1236I	CGCG	1 cut(s)	2337
Bsh1285I	CGRYCG	1 cut(s)	2666
BshNI	GGYRCC	2 cut(s)	593, 972
BsiEI	CGRYCG	1 cut(s)	2666
BsiHKAI	GWGCWC	8 cut(s)	40, 1591, 2321, 2738, 2814, 2828, 3181, 3863
BsiSI	CCGG	8 cut(s)	188, 233, 2330, 2662, 2791, 2840, 2957, 3174
BslFI	GGGAC	8 cut(s)	92, 129, 782, 2048, 2275, 2734, 3365, 3420
BsmBI	CGTCTC	4 cut(s)	138, 2712, 2751, 3399
BsmFI	GGGAC	8 cut(s)	92, 129, 782, 2048, 2275, 2734, 3365, 3420
BsmI	GAATGC	5 cut(s)	300, 1573, 2618, 3161, 3384
Bso31I	GGTCTC	3 cut(s)	241, 1758, 2662
Bsp119I	TTCGAA	2 cut(s)	311, 1663
Bsp13I	TCCGGA	1 cut(s)	2329
Bsp1720I	GCTNAGC	3 cut(s)	2181, 2433, 3207
Bsp19I	CCATGG	4 cut(s)	1353, 2090, 2270, 3765
Bsp68I	TCGCGA	1 cut(s)	2337
BspACI	CCGC	6 cut(s)	2291, 2361, 2673, 2700, 3029, 3149
BspEI	TCCGGA	1 cut(s)	2329
BspFNI	CGCG	1 cut(s)	2337
BspMAI	CTGCAG	2 cut(s)	676, 3699
BspMI	ACCTGC	2 cut(s)	1351, 3487
BspPI	GGATC	6 cut(s)	52, 1952, 2333, 2340, 2391, 3630
BspQI	GCTCTTC	1 cut(s)	2726
BspT104I	TTCGAA	2 cut(s)	311, 1663
BspT107I	GGYRCC	2 cut(s)	593, 972
BspTI	CTTAAG	1 cut(s)	2144
BspTNI	GGTCTC	3 cut(s)	241, 1758, 2662
BsrBI	CCGCTC	1 cut(s)	3031
BsrDI	GCAATG	4 cut(s)	1291, 1329, 3081, 3216
BsrFI	RCCGGY	2 cut(s)	2661, 2790
BsrI	ACTGG	7 cut(s)	511, 640, 759, 905, 1588, 1897, 2322
BssAI	RCCGGY	2 cut(s)	2661, 2790
BssNI	GRCGYC	4 cut(s)	1374, 2449, 2559, 2744
BssSI	CACGAG	1 cut(s)	3333
BssT1I	CCWWGG	6 cut(s)	1353, 2090, 2270, 3012, 3399, 3765
Bst2BI	CACGAG	1 cut(s)	3333
Bst4CI	ACNGT	8 cut(s)	1225, 1385, 1886, 2074, 2300, 2539, 2624, 3517
Bst6I	CTCTTC	8 cut(s)	260, 355, 1155, 2112, 2174, 2307, 2726, 3746
BstACI	GRCGYC	4 cut(s)	1374, 2449, 2559, 2744
BstAFI	CTTAAG	1 cut(s)	2144
BstAPI	GCANNNNNTGC	2 cut(s)	473, 680
BstBAI	YACGTR	2 cut(s)	736, 3924
BstBI	TTCGAA	2 cut(s)	311, 1663
BstC8I	GCNNGC	5 cut(s)	27, 2361, 2533, 2997, 3236
BstDSI	CCRYGG	4 cut(s)	1353, 2090, 2270, 3765
BstFNI	CGCG	1 cut(s)	2337
BstH2I	RGCGCY	1 cut(s)	3465
BstHHI	GCGC	3 cut(s)	300, 2296, 3464
BstMCI	CGRYCG	1 cut(s)	2666
BstMWI	GCNNNNNNNGC	8 cut(s)	35, 473, 494, 680, 752, 2662, 3677, 3716
BstNSI	RCATGY	4 cut(s)	131, 797, 1534, 1940
BstSFI	CTRYAG	3 cut(s)	672, 2427, 3695
BstSLI	GKGCMC	1 cut(s)	2814
BstUI	CGCG	1 cut(s)	2337
BstV2I	GAAGAC	2 cut(s)	21, 2357
BstX2I	RGATCY	3 cut(s)	44, 2633, 3622
BstXI	CCANNNNNNTGG	2 cut(s)	884, 1862
BstYI	RGATCY	3 cut(s)	44, 2633, 3622
BstZI	CGGCCG	1 cut(s)	2663
BsuI	GTATCC	1 cut(s)	1201
BtgI	CCRYGG	4 cut(s)	1353, 2090, 2270, 3765
BtsI	GCAGTG	1 cut(s)	2175
BtsIMutI	CAGTG	9 cut(s)	603, 766, 1234, 1390, 1545, 1731, 2175, 2329, 3513
BtuMI	TCGCGA	1 cut(s)	2337
BveI	ACCTGC	2 cut(s)	1351, 3487
Cac8I	GCNNGC	5 cut(s)	27, 2361, 2533, 2997, 3236
CaiI	CAGNNNCTG	1 cut(s)	680
CfoI	GCGC	3 cut(s)	300, 2296, 3464
Cfr10I	RCCGGY	2 cut(s)	2661, 2790
CseI	GACGC	2 cut(s)	2567, 2733
CsiI	ACCWGGT	1 cut(s)	2101
Csp6I	GTAC	3 cut(s)	650, 973, 2424
CspCI	CAANNNNNGTGG	2 cut(s)	1720, 1755
CviQI	GTAC	3 cut(s)	650, 973, 2424
DrdI	GACNNNNNNGTC	3 cut(s)	2917, 2983, 3526
DriI	GACNNNNNGTC	1 cut(s)	3265
DseDI	GACNNNNNNGTC	3 cut(s)	2917, 2983, 3526
EaeI	YGGCCR	3 cut(s)	688, 2663, 2841
EagI	CGGCCG	1 cut(s)	2663
Eam1104I	CTCTTC	8 cut(s)	260, 355, 1155, 2112, 2174, 2307, 2726, 3746
Eam1105I	GACNNNNNGTC	1 cut(s)	3265
EarI	CTCTTC	8 cut(s)	260, 355, 1155, 2112, 2174, 2307, 2726, 3746
Ecl136II	GAGCTC	6 cut(s)	38, 1589, 2319, 2736, 2826, 3861
EclXI	CGGCCG	1 cut(s)	2663
Eco130I	CCWWGG	6 cut(s)	1353, 2090, 2270, 3012, 3399, 3765
Eco24I	GRGCYC	9 cut(s)	40, 343, 1591, 2321, 2738, 2828, 3374, 3566, 3863
Eco31I	GGTCTC	3 cut(s)	241, 1758, 2662
Eco32I	GATATC	1 cut(s)	1763
Eco47I	GGWCC	9 cut(s)	1324, 2099, 2262, 2327, 2620, 2721, 3026, 3259, 3528
Eco52I	CGGCCG	1 cut(s)	2663
Eco53kI	GAGCTC	6 cut(s)	38, 1589, 2319, 2736, 2826, 3861
Eco57I	CTGAAG	3 cut(s)	33, 1569, 2658
EcoICRI	GAGCTC	6 cut(s)	38, 1589, 2319, 2736, 2826, 3861
EcoO109I	RGGNCCY	4 cut(s)	23, 2099, 2262, 2721
EcoRI	GAATTC	1 cut(s)	1659
EcoRV	GATATC	1 cut(s)	1763
EcoT14I	CCWWGG	6 cut(s)	1353, 2090, 2270, 3012, 3399, 3765
EcoT22I	ATGCAT	3 cut(s)	1300, 1575, 1848
EcoT38I	GRGCYC	9 cut(s)	40, 343, 1591, 2321, 2738, 2828, 3374, 3566, 3863
ErhI	CCWWGG	6 cut(s)	1353, 2090, 2270, 3012, 3399, 3765
Esp3I	CGTCTC	4 cut(s)	138, 2712, 2751, 3399
FalI	AAGNNNNNCTT	4 cut(s)	397, 429, 2021, 2053
FaqI	GGGAC	8 cut(s)	92, 129, 782, 2048, 2275, 2734, 3365, 3420
FauI	CCCGC	2 cut(s)	2368, 2680
FauNDI	CATATG	3 cut(s)	1238, 2125, 3897
FblI	GTMKAC	1 cut(s)	2007
FriOI	GRGCYC	9 cut(s)	40, 343, 1591, 2321, 2738, 2828, 3374, 3566, 3863
FspBI	CTAG	8 cut(s)	207, 1335, 1838, 2279, 2631, 2657, 2799, 3731
GlaI	GCGC	3 cut(s)	299, 2295, 3463
GsaI	CCCAGC	1 cut(s)	3138
GsuI	CTGGAG	8 cut(s)	1062, 1605, 1739, 1759, 1812, 1914, 2305, 3063
HaeII	RGCGCY	1 cut(s)	3465
HapII	CCGG	8 cut(s)	188, 233, 2330, 2662, 2791, 2840, 2957, 3174
HgaI	GACGC	2 cut(s)	2567, 2733
HhaI	GCGC	3 cut(s)	300, 2296, 3464
Hin1I	GRCGYC	4 cut(s)	1374, 2449, 2559, 2744
Hin6I	GCGC	3 cut(s)	298, 2294, 3462
HinP1I	GCGC	3 cut(s)	298, 2294, 3462
HincII	GTYRAC	1 cut(s)	2233
HindII	GTYRAC	1 cut(s)	2233
HindIII	AAGCTT	1 cut(s)	3296
HpaII	CCGG	8 cut(s)	188, 233, 2330, 2662, 2791, 2840, 2957, 3174
Hpy166II	GTNNAC	5 cut(s)	1462, 1493, 2008, 2233, 2812
Hpy8I	GTNNAC	5 cut(s)	1462, 1493, 2008, 2233, 2812
Hpy99I	CGWCG	6 cut(s)	2397, 2451, 2454, 2561, 2628, 2923
HpyCH4III	ACNGT	8 cut(s)	1225, 1385, 1886, 2074, 2300, 2539, 2624, 3517
HpyCH4IV	ACGT	8 cut(s)	735, 1374, 1858, 2449, 2912, 3428, 3645, 3923
HpyF10VI	GCNNNNNNNGC	8 cut(s)	35, 473, 494, 680, 752, 2662, 3677, 3716
HpySE526I	ACGT	8 cut(s)	735, 1374, 1858, 2449, 2912, 3428, 3645, 3923
Hsp92I	GRCGYC	4 cut(s)	1374, 2449, 2559, 2744
HspAI	GCGC	3 cut(s)	298, 2294, 3462
KflI	GGGWCCC	1 cut(s)	2262
Kpn2I	TCCGGA	1 cut(s)	2329
KpnI	GGTACC	1 cut(s)	976
LguI	GCTCTTC	1 cut(s)	2726
MabI	ACCWGGT	1 cut(s)	2101
MaeI	CTAG	8 cut(s)	207, 1335, 1838, 2279, 2631, 2657, 2799, 3731
MaeII	ACGT	8 cut(s)	735, 1374, 1858, 2449, 2912, 3428, 3645, 3923
MbiI	CCGCTC	1 cut(s)	3031
MfeI	CAATTG	1 cut(s)	867
MflI	RGATCY	3 cut(s)	44, 2633, 3622
MlsI	TGGCCA	1 cut(s)	690
MluNI	TGGCCA	1 cut(s)	690
MlyI	GAGTC	4 cut(s)	341, 1103, 3290, 3883
MmeI	TCCRAC	4 cut(s)	1587, 1985, 2801, 3237
Mox20I	TGGCCA	1 cut(s)	690
Mph1103I	ATGCAT	3 cut(s)	1300, 1575, 1848
MroI	TCCGGA	1 cut(s)	2329
MroXI	GAANNNNTTC	1 cut(s)	1698
MscI	TGGCCA	1 cut(s)	690
MseI	TTAA	7 cut(s)	519, 1073, 1263, 2145, 2726, 3309, 3657
MslI	CAYNNNNRTG	3 cut(s)	531, 591, 2089
Msp20I	TGGCCA	1 cut(s)	690
MspA1I	CMGCKG	4 cut(s)	470, 666, 3134, 3149
MspCI	CTTAAG	1 cut(s)	2144
MspI	CCGG	8 cut(s)	188, 233, 2330, 2662, 2791, 2840, 2957, 3174
MunI	CAATTG	1 cut(s)	867
Mva1269I	GAATGC	5 cut(s)	300, 1573, 2618, 3161, 3384
MvnI	CGCG	1 cut(s)	2337
MwoI	GCNNNNNNNGC	8 cut(s)	35, 473, 494, 680, 752, 2662, 3677, 3716
NcoI	CCATGG	4 cut(s)	1353, 2090, 2270, 3765
NdeI	CATATG	3 cut(s)	1238, 2125, 3897
NmeAIII	GCCGAG	3 cut(s)	2267, 2691, 3012
NmuCI	GTSAC	8 cut(s)	1667, 2082, 2300, 2489, 2695, 2974, 3511, 3517
NruI	TCGCGA	1 cut(s)	2337
NsiI	ATGCAT	3 cut(s)	1300, 1575, 1848
NspI	RCATGY	4 cut(s)	131, 797, 1534, 1940
NspV	TTCGAA	2 cut(s)	311, 1663
OliI	CACNNNNGTG	1 cut(s)	591
PasI	CCCWGGG	1 cut(s)	112
PciI	ACATGT	1 cut(s)	1936
PciSI	GCTCTTC	1 cut(s)	2726
PcsI	WCGNNNNNNNCGW	4 cut(s)	2007, 2918, 2984, 3889
PctI	GAATGC	5 cut(s)	300, 1573, 2618, 3161, 3384
PdmI	GAANNNNTTC	1 cut(s)	1698
PflFI	GACNNNGTC	1 cut(s)	2448
PflMI	CCANNNNNTGG	2 cut(s)	1354, 2156
PfoI	TCCNGGA	3 cut(s)	1320, 2773, 3040
PleI	GAGTC	4 cut(s)	341, 1103, 3289, 3883
PpsI	GAGTC	4 cut(s)	341, 1103, 3289, 3883
Ppu21I	YACGTR	2 cut(s)	736, 3924
PpuMI	RGGWCCY	3 cut(s)	2099, 2262, 2721
PscI	ACATGT	1 cut(s)	1936
Psp124BI	GAGCTC	6 cut(s)	40, 1591, 2321, 2738, 2828, 3863
Psp1406I	AACGTT	2 cut(s)	1858, 3645
Psp5II	RGGWCCY	3 cut(s)	2099, 2262, 2721
PspFI	CCCAGC	1 cut(s)	3134
PspPPI	RGGWCCY	3 cut(s)	2099, 2262, 2721
PstI	CTGCAG	2 cut(s)	676, 3699
PstNI	CAGNNNCTG	1 cut(s)	680
PsuI	RGATCY	3 cut(s)	44, 2633, 3622
PsyI	GACNNNGTC	1 cut(s)	2448
PvuII	CAGCTG	3 cut(s)	470, 666, 3134
RruI	TCGCGA	1 cut(s)	2337
RsaI	GTAC	3 cut(s)	651, 974, 2425
RsaNI	GTAC	3 cut(s)	650, 973, 2424
RseI	CAYNNNNRTG	3 cut(s)	531, 591, 2089
SacI	GAGCTC	6 cut(s)	40, 1591, 2321, 2738, 2828, 3863
SapI	GCTCTTC	1 cut(s)	2726
SaqAI	TTAA	7 cut(s)	519, 1073, 1263, 2145, 2726, 3309, 3657
SchI	GAGTC	4 cut(s)	341, 1103, 3290, 3883
SexAI	ACCWGGT	1 cut(s)	2101
SfcI	CTRYAG	3 cut(s)	672, 2427, 3695
SfuI	TTCGAA	2 cut(s)	311, 1663
SinI	GGWCC	9 cut(s)	1324, 2099, 2262, 2327, 2620, 2721, 3026, 3259, 3528
SmiMI	CAYNNNNRTG	3 cut(s)	531, 591, 2089
SmlI	CTYRAG	6 cut(s)	276, 321, 854, 2144, 3285, 3810
SmoI	CTYRAG	6 cut(s)	276, 321, 854, 2144, 3285, 3810
SpeI	ACTAGT	1 cut(s)	2798
SsiI	CCGC	6 cut(s)	2291, 2361, 2673, 2700, 3029, 3149
SspI	AATATT	2 cut(s)	949, 1748
SspMI	CTAG	8 cut(s)	207, 1335, 1838, 2279, 2631, 2657, 2799, 3731
SstI	GAGCTC	6 cut(s)	40, 1591, 2321, 2738, 2828, 3863
StyI	CCWWGG	6 cut(s)	1353, 2090, 2270, 3012, 3399, 3765
TaaI	ACNGT	8 cut(s)	1225, 1385, 1886, 2074, 2300, 2539, 2624, 3517
TaiI	ACGT	8 cut(s)	738, 1377, 1861, 2452, 2915, 3431, 3648, 3926
TaqI	TCGA	7 cut(s)	311, 1663, 2395, 2626, 2921, 2987, 3607
TatI	WGTACW	1 cut(s)	649
TauI	GCSGC	1 cut(s)	2294
Tru1I	TTAA	7 cut(s)	519, 1073, 1263, 2145, 2726, 3309, 3657
Tru9I	TTAA	7 cut(s)	519, 1073, 1263, 2145, 2726, 3309, 3657
TscAI	CASTG	9 cut(s)	610, 766, 1234, 1390, 1552, 1738, 2182, 2329, 3520
TseFI	GTSAC	8 cut(s)	1667, 2082, 2300, 2489, 2695, 2974, 3511, 3517
Tsp45I	GTSAC	8 cut(s)	1667, 2082, 2300, 2489, 2695, 2974, 3511, 3517
TspGWI	ACGGA	5 cut(s)	716, 1654, 2016, 3114, 3739
TspRI	CASTG	9 cut(s)	610, 766, 1234, 1390, 1552, 1738, 2182, 2329, 3520
Tth111I	GACNNNGTC	1 cut(s)	2448
Van91I	CCANNNNNTGG	2 cut(s)	1354, 2156
Vha464I	CTTAAG	1 cut(s)	2144
VneI	GTGCAC	1 cut(s)	2810
VpaK11BI	GGWCC	9 cut(s)	1324, 2099, 2262, 2327, 2620, 2721, 3026, 3259, 3528
XapI	RAATTY	3 cut(s)	1476, 1659, 1676
XbaI	TCTAGA	1 cut(s)	2630
XceI	RCATGY	4 cut(s)	131, 797, 1534, 1940
XcmI	CCANNNNNNNNNTGG	2 cut(s)	361, 2851
XmiI	GTMKAC	1 cut(s)	2007
XmnI	GAANNNNTTC	1 cut(s)	1698
XspI	CTAG	8 cut(s)	207, 1335, 1838, 2279, 2631, 2657, 2799, 3731
ZraI	GACGTC	2 cut(s)	1375, 2450
Zsp2I	ATGCAT	3 cut(s)	1300, 1575, 1848

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.