Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0014673

Physical Location & Seq

Genomic Coordinates Forward (+)

66189136 .. 66194030

Gene Structure 4895 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ27697

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2346 bp

ATGGTTGTTGAAGCTGAAGACGAGGGGCCTGCTGTGAGAGCTCTCGGATCTCTGTTGAAGCTCACCCAAGTCTTTCTCTGGGACGATGGCACAAGAAACGACAGGTCGTTTTCCCTGGGACAGACTAAACCAGCTCAGGATGAAGGAGACGATGGCTCAAACGCTCTTTCCTCAGAATGTGGAACTTTGCCGGAGGATATGGAACTTACTAGACAGATGAATGAGTTGGGGCTTCCGGTCTCATTTCGCACAAATAAGGAGAAGCAGAAGAGAACAACTCAAGGAAAAAGAAAAGGAATGCGCCAGAAGCATTCGAACGAATCTCAAGAAGTTGTGGGCGGAGCCTTGGACTCTTCCAAAGTGAGTGTGGAGGAGATTGTATCTCCTATTATATTTGATGATAACCCAAGCAGTTCTTTGTGTTGTATGTCAATGATGGGCCAAAGTGAATCATCTTCCTCTGATGTTGCAGCTGATGCCAATAATCCAGCAAGTTCAGCTAAGATTACTGGTGATTCGCGTGATGGAATAGTCGGCATTTTTTCTAATGAAGGCCAGGATTGTGATTCCTTACACAATGGTGCCATGCTCACTGATGCAACAACAATTTTAGCTAGTTCAACTGGGTTAGATGCTGTACTTTACCCAGGCAGCTGCTCTGCAGATGCTGCTAATGGCCATGATAAGACAGAGCCAGGTGAAAGACTTATGGAGCATGACCACATAGAGTGTGCAATAATGGCTACCAGTGAAGCAGAAATAACCGAAAGTTGTGAGGAGCATGTCCCTGAGCAGCCATGTGTTTCTGAATCAGTCTCATGTTCCATATATCCAGAAGGTCTTGAGAATGATGCAATTGACAGCCAACAAAGTGGTGATTTTGGAGACTGGATGGTATATTGGGATTCTTACTACATGAGAAATTACTTCTATAATATTAGAACACATACTTCAACATGGTACCCACCCGTGGGCATGGAACATTTAGAAAATGTTGATAGCACATATAAGTCAAATGAAGGTGTTGCTGGAGTAACTCAGATGGATGTTACCACAGACTTAAAGGCAACAGATTTATGTGTTTTGAGTAAGTTTGACTCATTTGAAGAACCAATCAATGTTGATGTTTTACAATATCAACGATATGAAGAGGTTTCAAGGGATGTTGAAATTACTGCTGCCAATACTTTATTGGATACATCCACTTCAACTGTCAGTGTAAGCATATGTCCTGAGCTTCCAGATGAACTTAATCAGAGCAATAACAGGTGCAATGATGGAAATGCATCATGCTTGTCATCAAATGTCCAGGACCACATTGCTAGTTGCAGAAACCAAACCATGGAGCAGGTTGCTGATGAGGTCTGCAACAGTGATTTGCAACCAATTTTTGCAGGAAAAAGTGCTGAACCAGATATTTCTGATCTCTATGATAAACCTAATGAAGTTTACTTTTGTGAGGAAATTCCTAAATATGGTGAACATGATGAGGCGATTCAAACATTAGATACAAGCAGCATGTCCAATTCATGCACTGAAGAAGTTGAGGATTGGAATATGCATTCTGAAACTGGAGTTCCTGCAACAAATGAGTTGGAAATGAAATCTGACCCTGCTTTAGTAAAACGGAAAAAGAAAGTGAGAAGAATTCGAAGTCACAGAAAATTCTTCAACGAGAATGAAGAAGTTCTATTTGAAGGATTGCTGAAGAAGTGTTCCACTGATATCGGTAAATATTGGTCTCAGAGATATCTATTATTCTCCAGATATGATGATGGTATAAAAATGGATGAGGAAGGATGGTTTTCAGTTACTCCAGAAGCTTTAGCTATGCATCATGCCCAACGTTGTGGTAGTGGCATTATAATTGACTGTTTTACTGGAGTTGGTGGTAATGCCATCCAATTTGCTCAGATAAGCAAACATGTAACTGCCATTGATATTGATCCAACAAAGATTGATTATGCTCAACATAATGCTGCTATCTACGGAGTAGACGAAAGGATAGATTTCATAAAGGGGGACTTTTTCCATTTGGCATCAAGGTTGAAGGCAGATACAGTCTTTTTGTCACCACCATGGGGAGGACCTGGTTATGCAAGAGAAGAGACATATGACATTATGACAATGCTTAAGCCACATGATGGATATTTTCTCTTCAACACTGCTAAGCAAGTTGCTTCCAAGATTGTCATGTTTCTCCCAAGAAATGTTGATGTCAACCAATTAGCAGAGTTGTCTCTTTCAGGGACTCCACCATGGTCGCTAGAGGTGGAGAAAAACTTTGTAAATGGCAAGTTGAAGGGAATAACTGCTTACTTCAGTGACACGGCAAGGAGAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016741 GO:0032259 GO:0034641 GO:0036260 GO:0036261 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071164 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Methyltransf_15 WW

Protein Analysis

781

Amino Acids

86.51

Weight (kDa)

4.55

Isoelectric Point (pI)

44.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_15	PF09445	621 - 775	3.7e-43	RNA cap guanine-N2 methyltransferase
TRM5-TYW2_MTfase	PF02475	621 - 691	2.9e-07	TRM5/TYW2 methyltransferase domain
Methyltransf_31	PF13847	622 - 724	2.6e-07	Methyltransferase domain
Methyltransf_25	PF13649	622 - 689	7.4e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014673)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34310 FvH4_2g34310
malus_domestica	MD15G1081500.v1.1
prunus_persica	Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1
pyrus_communis	pycom15g07620 pycom15g07630
rosa_chinensis	RchiOBHm_Chr6g0308051
rosa_laevigata	RLG00000010672
rosa_multiflora	Rmu_sc0002624.1_g000003
rosa_roxburghii	Rroxscaffold_7G00160630
rosa_rugosa	Rorug06G0363400
rosa_samantha	Rh6AG472300 Rh6BG483400 Rh6DG474700
rosa_wichuraiana	Rw6G041220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1865
Acc36I	ACCTGC	1 cut(s)	1339
Acc65I	GGTACC	1 cut(s)	960
AccB1I	GGYRCC	2 cut(s)	581, 960
AccB7I	CCANNNNNTGG	2 cut(s)	1342, 2144
AccI	GTMKAC	1 cut(s)	1995
AccII	CGCG	1 cut(s)	520
AciI	CCGC	1 cut(s)	339
AclI	AACGTT	1 cut(s)	1846
AclWI	GGATC	2 cut(s)	55, 1940
AcoI	YGGCCR	1 cut(s)	676
AcsI	RAATTY	3 cut(s)	1464, 1647, 1664
AcuI	CTGAAG	4 cut(s)	36, 1557, 1727, 2305
AfaI	GTAC	2 cut(s)	639, 962
AfiI	CCNNNNNNNGG	7 cut(s)	970, 971, 1342, 1475, 2081, 2084, 2144
AflII	CTTAAG	1 cut(s)	2132
AflIII	ACRYGT	1 cut(s)	1924
AjnI	CCWGG	6 cut(s)	114, 555, 646, 694, 1308, 2089
AleI	CACNNNNGTG	1 cut(s)	579
Alw21I	GWGCWC	1 cut(s)	43
Alw26I	GTCTC	7 cut(s)	141, 244, 820, 879, 1746, 2102, 2244
AlwI	GGATC	2 cut(s)	55, 1940
AlwNI	CAGNNNCTG	1 cut(s)	668
AoxI	GGCC	4 cut(s)	26, 439, 553, 676
ApeKI	GCWGC	8 cut(s)	470, 651, 654, 668, 793, 1178, 1515, 1979
ApoI	RAATTY	3 cut(s)	1464, 1647, 1664
Asp700I	GAANNNNTTC	1 cut(s)	1686
Asp718I	GGTACC	1 cut(s)	960
AspLEI	GCGC	1 cut(s)	303
AspS9I	GGNCC	4 cut(s)	26, 439, 1312, 2087
AsuHPI	GGTGA	6 cut(s)	55, 524, 710, 887, 1490, 2064
AsuII	TTCGAA	2 cut(s)	314, 1651
AvaII	GGWCC	2 cut(s)	1312, 2087
BalI	TGGCCA	1 cut(s)	678
BanI	GGYRCC	2 cut(s)	581, 960
BanII	GRGCYC	1 cut(s)	43
BbsI	GAAGAC	1 cut(s)	24
Bbv12I	GWGCWC	1 cut(s)	43
BbvI	GCAGC	8 cut(s)	482, 641, 655, 663, 805, 1165, 1527, 1966
BcgI	CGANNNNNNTGC	2 cut(s)	11, 45
BciT130I	CCWGG	6 cut(s)	116, 557, 648, 696, 1310, 2091
BciVI	GTATCC	1 cut(s)	1189
BcoDI	GTCTC	7 cut(s)	141, 244, 820, 879, 1746, 2102, 2244
BfaI	CTAG	4 cut(s)	210, 615, 1323, 2267
BfmI	CTRYAG	1 cut(s)	660
BfrI	CTTAAG	1 cut(s)	2132
BfuAI	ACCTGC	1 cut(s)	1339
BfuI	GTATCC	1 cut(s)	1189
BisI	GCNGC	8 cut(s)	471, 652, 655, 669, 794, 1179, 1516, 1980
BlpI	GCTNAGC	1 cut(s)	2169
BlsI	GCNGC	8 cut(s)	472, 653, 656, 670, 795, 1180, 1517, 1981
Bme1390I	CCNGG	6 cut(s)	116, 557, 648, 696, 1310, 2091
Bme18I	GGWCC	2 cut(s)	1312, 2087
BmgT120I	GGNCC	4 cut(s)	26, 439, 1312, 2087
BmiI	GGNNCC	4 cut(s)	27, 343, 583, 962
BmrFI	CCNGG	6 cut(s)	116, 557, 648, 696, 1310, 2091
BmrI	ACTGGG	1 cut(s)	633
BmsI	GCATC	8 cut(s)	466, 586, 622, 655, 841, 1295, 1843, 2048
BmuI	ACTGGG	1 cut(s)	633
BpiI	GAAGAC	1 cut(s)	24
BplI	GAGNNNNNCTC	2 cut(s)	262, 294
BpmI	CTGGAG	5 cut(s)	1050, 1593, 1747, 1800, 1902
Bpu10I	CCTNAGC	3 cut(s)	135, 789, 1233
Bpu1102I	GCTNAGC	1 cut(s)	2169
Bpu14I	TTCGAA	2 cut(s)	314, 1651
BpuEI	CTTGAG	3 cut(s)	264, 309, 863
BsaBI	GATNNNNATC	1 cut(s)	1944
BsaI	GGTCTC	2 cut(s)	244, 1746
BsaJI	CCNNGG	8 cut(s)	114, 115, 345, 646, 969, 1341, 2078, 2258
BsaWI	WCCGGW	1 cut(s)	235
Bsc4I	CCNNNNNNNGG	7 cut(s)	970, 971, 1342, 1475, 2081, 2084, 2144
Bse1I	ACTGG	6 cut(s)	514, 628, 747, 893, 1576, 1885
Bse3DI	GCAATG	2 cut(s)	1279, 1317
Bse8I	GATNNNNATC	1 cut(s)	1944
BseBI	CCWGG	6 cut(s)	116, 557, 648, 696, 1310, 2091
BseDI	CCNNGG	8 cut(s)	114, 115, 345, 646, 969, 1341, 2078, 2258
BseGI	GGATG	8 cut(s)	145, 897, 1051, 1168, 1199, 1795, 1805, 1899
BseJI	GATNNNNATC	1 cut(s)	1944
BseLI	CCNNNNNNNGG	7 cut(s)	970, 971, 1342, 1475, 2081, 2084, 2144
BseMI	GCAATG	2 cut(s)	1279, 1317
BseMII	CTCAG	7 cut(s)	149, 186, 780, 1052, 1224, 1757, 1925
BseNI	ACTGG	6 cut(s)	514, 628, 747, 893, 1576, 1885
BseRI	GAGGAG	2 cut(s)	386, 791
BseXI	GCAGC	8 cut(s)	482, 641, 655, 663, 805, 1165, 1527, 1966
Bsh1236I	CGCG	1 cut(s)	520
BshFI	GGCC	4 cut(s)	28, 441, 555, 678
BshNI	GGYRCC	2 cut(s)	581, 960
BsiHKAI	GWGCWC	1 cut(s)	43
BsiSI	CCGG	2 cut(s)	191, 236
BslFI	GGGAC	5 cut(s)	95, 132, 770, 2036, 2263
BslI	CCNNNNNNNGG	7 cut(s)	970, 971, 1342, 1475, 2081, 2084, 2144
BsmAI	GTCTC	7 cut(s)	141, 244, 820, 879, 1746, 2102, 2244
BsmBI	CGTCTC	1 cut(s)	141
BsmFI	GGGAC	5 cut(s)	95, 132, 770, 2036, 2263
BsmI	GAATGC	3 cut(s)	303, 310, 1561
BsnI	GGCC	4 cut(s)	28, 441, 555, 678
Bso31I	GGTCTC	2 cut(s)	244, 1746
Bsp119I	TTCGAA	2 cut(s)	314, 1651
Bsp1286I	GDGCHC	1 cut(s)	43
Bsp143I	GATC	3 cut(s)	47, 1423, 1945
Bsp1720I	GCTNAGC	1 cut(s)	2169
Bsp19I	CCATGG	3 cut(s)	1341, 2078, 2258
BspACI	CCGC	1 cut(s)	339
BspANI	GGCC	4 cut(s)	28, 441, 555, 678
BspCNI	CTCAG	7 cut(s)	148, 185, 781, 1051, 1225, 1756, 1924
BspFNI	CGCG	1 cut(s)	520
BspLI	GGNNCC	4 cut(s)	27, 343, 583, 962
BspMAI	CTGCAG	1 cut(s)	664
BspMI	ACCTGC	1 cut(s)	1339
BspPI	GGATC	2 cut(s)	55, 1940
BspT104I	TTCGAA	2 cut(s)	314, 1651
BspT107I	GGYRCC	2 cut(s)	581, 960
BspTI	CTTAAG	1 cut(s)	2132
BspTNI	GGTCTC	2 cut(s)	244, 1746
BsrDI	GCAATG	2 cut(s)	1279, 1317
BsrI	ACTGG	6 cut(s)	514, 628, 747, 893, 1576, 1885
BssECI	CCNNGG	8 cut(s)	114, 115, 345, 646, 969, 1341, 2078, 2258
BssMI	GATC	3 cut(s)	47, 1423, 1945
BssT1I	CCWWGG	4 cut(s)	345, 1341, 2078, 2258
Bst2UI	CCWGG	6 cut(s)	116, 557, 648, 696, 1310, 2091
Bst4CI	ACNGT	4 cut(s)	1213, 1373, 1874, 2062
Bst6I	CTCTTC	5 cut(s)	263, 358, 1143, 2100, 2162
BstAFI	CTTAAG	1 cut(s)	2132
BstAPI	GCANNNNNTGC	2 cut(s)	476, 668
BstBI	TTCGAA	2 cut(s)	314, 1651
BstC8I	GCNNGC	1 cut(s)	30
BstDEI	CTNAG	9 cut(s)	135, 172, 501, 789, 1038, 1233, 1743, 1911, 2169
BstDSI	CCRYGG	4 cut(s)	969, 1341, 2078, 2258
BstF5I	GGATG	8 cut(s)	145, 897, 1051, 1168, 1199, 1795, 1805, 1899
BstFNI	CGCG	1 cut(s)	520
BstHHI	GCGC	1 cut(s)	303
BstKTI	GATC	3 cut(s)	50, 1426, 1948
BstMAI	GTCTC	7 cut(s)	141, 244, 820, 879, 1746, 2102, 2244
BstMBI	GATC	3 cut(s)	47, 1423, 1945
BstMWI	GCNNNNNNNGC	6 cut(s)	38, 307, 476, 497, 668, 740
BstNI	CCWGG	6 cut(s)	116, 557, 648, 696, 1310, 2091
BstNSI	RCATGY	3 cut(s)	785, 1522, 1928
BstSCI	CCNGG	6 cut(s)	114, 555, 646, 694, 1308, 2089
BstSFI	CTRYAG	1 cut(s)	660
BstUI	CGCG	1 cut(s)	520
BstV1I	GCAGC	8 cut(s)	482, 641, 655, 663, 805, 1165, 1527, 1966
BstV2I	GAAGAC	1 cut(s)	24
BstX2I	RGATCY	1 cut(s)	47
BstXI	CCANNNNNNTGG	2 cut(s)	872, 1850
BstYI	RGATCY	1 cut(s)	47
BsuI	GTATCC	1 cut(s)	1189
BsuRI	GGCC	4 cut(s)	28, 441, 555, 678
BtgI	CCRYGG	4 cut(s)	969, 1341, 2078, 2258
BtsCI	GGATG	8 cut(s)	145, 897, 1051, 1168, 1199, 1795, 1805, 1899
BtsI	GCAGTG	1 cut(s)	2163
BtsIMutI	CAGTG	8 cut(s)	591, 754, 1222, 1378, 1533, 1719, 2163, 2329
BveI	ACCTGC	1 cut(s)	1339
Cac8I	GCNNGC	1 cut(s)	30
CaiI	CAGNNNCTG	1 cut(s)	668
CfoI	GCGC	1 cut(s)	303
Cfr13I	GGNCC	4 cut(s)	26, 439, 1312, 2087
CsiI	ACCWGGT	1 cut(s)	2089
Csp6I	GTAC	2 cut(s)	638, 961
CviQI	GTAC	2 cut(s)	638, 961
DdeI	CTNAG	9 cut(s)	135, 172, 501, 789, 1038, 1233, 1743, 1911, 2169
DpnI	GATC	3 cut(s)	49, 1425, 1947
DpnII	GATC	3 cut(s)	47, 1423, 1945
EaeI	YGGCCR	1 cut(s)	676
Eam1104I	CTCTTC	5 cut(s)	263, 358, 1143, 2100, 2162
EarI	CTCTTC	5 cut(s)	263, 358, 1143, 2100, 2162
EciI	GGCGGA	1 cut(s)	354
Ecl136II	GAGCTC	1 cut(s)	41
Eco130I	CCWWGG	4 cut(s)	345, 1341, 2078, 2258
Eco24I	GRGCYC	1 cut(s)	43
Eco31I	GGTCTC	2 cut(s)	244, 1746
Eco32I	GATATC	2 cut(s)	1726, 1751
Eco47I	GGWCC	2 cut(s)	1312, 2087
Eco53kI	GAGCTC	1 cut(s)	41
Eco57I	CTGAAG	4 cut(s)	36, 1557, 1727, 2305
EcoICRI	GAGCTC	1 cut(s)	41
EcoO109I	RGGNCCY	2 cut(s)	26, 2087
EcoRI	GAATTC	1 cut(s)	1647
EcoRII	CCWGG	6 cut(s)	114, 555, 646, 694, 1308, 2089
EcoRV	GATATC	2 cut(s)	1726, 1751
EcoT14I	CCWWGG	4 cut(s)	345, 1341, 2078, 2258
EcoT22I	ATGCAT	3 cut(s)	1288, 1563, 1836
EcoT38I	GRGCYC	1 cut(s)	43
ErhI	CCWWGG	4 cut(s)	345, 1341, 2078, 2258
Esp3I	CGTCTC	1 cut(s)	141
FalI	AAGNNNNNCTT	4 cut(s)	400, 432, 2009, 2041
FaqI	GGGAC	5 cut(s)	95, 132, 770, 2036, 2263
FauNDI	CATATG	2 cut(s)	1226, 2113
FblI	GTMKAC	1 cut(s)	1995
Fnu4HI	GCNGC	8 cut(s)	471, 652, 655, 669, 794, 1179, 1516, 1980
FokI	GGATG	8 cut(s)	152, 904, 1058, 1175, 1186, 1802, 1812, 1886
FriOI	GRGCYC	1 cut(s)	43
Fsp4HI	GCNGC	8 cut(s)	471, 652, 655, 669, 794, 1179, 1516, 1980
FspBI	CTAG	4 cut(s)	210, 615, 1323, 2267
GlaI	GCGC	1 cut(s)	302
GluI	GCNGC	8 cut(s)	471, 652, 655, 669, 794, 1179, 1516, 1980
GsuI	CTGGAG	5 cut(s)	1050, 1593, 1747, 1800, 1902
HaeIII	GGCC	4 cut(s)	28, 441, 555, 678
HapII	CCGG	2 cut(s)	191, 236
HhaI	GCGC	1 cut(s)	303
Hin6I	GCGC	1 cut(s)	301
HinP1I	GCGC	1 cut(s)	301
HincII	GTYRAC	1 cut(s)	2221
HindII	GTYRAC	1 cut(s)	2221
HindIII	AAGCTT	1 cut(s)	1821
HpaII	CCGG	2 cut(s)	191, 236
HphI	GGTGA	6 cut(s)	55, 524, 710, 887, 1490, 2064
Hpy166II	GTNNAC	4 cut(s)	1450, 1481, 1996, 2221
Hpy188III	TCNNGA	8 cut(s)	137, 326, 833, 842, 1232, 1241, 1764, 1817
Hpy8I	GTNNAC	4 cut(s)	1450, 1481, 1996, 2221
HpyAV	CCTTC	8 cut(s)	137, 545, 830, 1013, 1691, 1790, 2044, 2296
HpyCH4III	ACNGT	4 cut(s)	1213, 1373, 1874, 2062
HpyCH4IV	ACGT	1 cut(s)	1846
HpyF10VI	GCNNNNNNNGC	6 cut(s)	38, 307, 476, 497, 668, 740
HpyF3I	CTNAG	9 cut(s)	135, 172, 501, 789, 1038, 1233, 1743, 1911, 2169
HpySE526I	ACGT	1 cut(s)	1846
HspAI	GCGC	1 cut(s)	301
KpnI	GGTACC	1 cut(s)	964
Kzo9I	GATC	3 cut(s)	47, 1423, 1945
LmnI	GCTCC	4 cut(s)	341, 712, 778, 1345
Lsp1109I	GCAGC	8 cut(s)	482, 641, 655, 663, 805, 1165, 1527, 1966
LweI	GCATC	8 cut(s)	466, 586, 622, 655, 841, 1295, 1843, 2048
MabI	ACCWGGT	1 cut(s)	2089
MaeI	CTAG	4 cut(s)	210, 615, 1323, 2267
MaeII	ACGT	1 cut(s)	1846
MaeIII	GTNAC	7 cut(s)	1033, 1048, 1655, 1810, 1927, 2070, 2324
MalI	GATC	3 cut(s)	49, 1425, 1947
MboI	GATC	3 cut(s)	47, 1423, 1945
MfeI	CAATTG	1 cut(s)	855
MflI	RGATCY	1 cut(s)	47
MhlI	GDGCHC	1 cut(s)	43
MlsI	TGGCCA	1 cut(s)	678
MluNI	TGGCCA	1 cut(s)	678
MlyI	GAGTC	3 cut(s)	344, 1091, 2245
MmeI	TCCRAC	2 cut(s)	1575, 1973
Mox20I	TGGCCA	1 cut(s)	678
Mph1103I	ATGCAT	3 cut(s)	1288, 1563, 1836
MroXI	GAANNNNTTC	1 cut(s)	1686
MscI	TGGCCA	1 cut(s)	678
MseI	TTAA	3 cut(s)	1061, 1251, 2133
MslI	CAYNNNNRTG	2 cut(s)	579, 2077
Msp20I	TGGCCA	1 cut(s)	678
MspA1I	CMGCKG	2 cut(s)	473, 654
MspCI	CTTAAG	1 cut(s)	2132
MspI	CCGG	2 cut(s)	191, 236
MspR9I	CCNGG	6 cut(s)	116, 557, 648, 696, 1310, 2091
MunI	CAATTG	1 cut(s)	855
Mva1269I	GAATGC	3 cut(s)	303, 310, 1561
MvaI	CCWGG	6 cut(s)	116, 557, 648, 696, 1310, 2091
MvnI	CGCG	1 cut(s)	520
MwoI	GCNNNNNNNGC	6 cut(s)	38, 307, 476, 497, 668, 740
NcoI	CCATGG	3 cut(s)	1341, 2078, 2258
NdeI	CATATG	2 cut(s)	1226, 2113
NdeII	GATC	3 cut(s)	47, 1423, 1945
NlaIV	GGNNCC	4 cut(s)	27, 343, 583, 962
NmuCI	GTSAC	3 cut(s)	1655, 2070, 2324
NsiI	ATGCAT	3 cut(s)	1288, 1563, 1836
NspI	RCATGY	3 cut(s)	785, 1522, 1928
NspV	TTCGAA	2 cut(s)	314, 1651
OliI	CACNNNNGTG	1 cut(s)	579
PasI	CCCWGGG	1 cut(s)	115
PciI	ACATGT	1 cut(s)	1924
PcsI	WCGNNNNNNNCGW	1 cut(s)	1995
PctI	GAATGC	3 cut(s)	303, 310, 1561
PdmI	GAANNNNTTC	1 cut(s)	1686
PfeI	GAWTC	7 cut(s)	320, 449, 515, 566, 809, 905, 1495
PflMI	CCANNNNNTGG	2 cut(s)	1342, 2144
PfoI	TCCNGGA	1 cut(s)	1308
PkrI	GCNGC	8 cut(s)	472, 653, 656, 670, 795, 1180, 1517, 1981
PleI	GAGTC	3 cut(s)	344, 1091, 2245
PpsI	GAGTC	3 cut(s)	344, 1091, 2245
PpuMI	RGGWCCY	1 cut(s)	2087
PscI	ACATGT	1 cut(s)	1924
PsiI	TTATAA	1 cut(s)	1865
Psp124BI	GAGCTC	1 cut(s)	43
Psp1406I	AACGTT	1 cut(s)	1846
Psp5II	RGGWCCY	1 cut(s)	2087
Psp6I	CCWGG	6 cut(s)	114, 555, 646, 694, 1308, 2089
PspGI	CCWGG	6 cut(s)	114, 555, 646, 694, 1308, 2089
PspN4I	GGNNCC	4 cut(s)	27, 343, 583, 962
PspPI	GGNCC	4 cut(s)	26, 439, 1312, 2087
PspPPI	RGGWCCY	1 cut(s)	2087
PstI	CTGCAG	1 cut(s)	664
PstNI	CAGNNNCTG	1 cut(s)	668
PsuI	RGATCY	1 cut(s)	47
PvuII	CAGCTG	2 cut(s)	473, 654
RsaI	GTAC	2 cut(s)	639, 962
RsaNI	GTAC	2 cut(s)	638, 961
RseI	CAYNNNNRTG	2 cut(s)	579, 2077
SacI	GAGCTC	1 cut(s)	43
SaqAI	TTAA	3 cut(s)	1061, 1251, 2133
SatI	GCNGC	8 cut(s)	471, 652, 655, 669, 794, 1179, 1516, 1980
Sau3AI	GATC	3 cut(s)	47, 1423, 1945
Sau96I	GGNCC	4 cut(s)	26, 439, 1312, 2087
SchI	GAGTC	3 cut(s)	344, 1091, 2245
ScrFI	CCNGG	6 cut(s)	116, 557, 648, 696, 1310, 2091
SduI	GDGCHC	1 cut(s)	43
SexAI	ACCWGGT	1 cut(s)	2089
SfaNI	GCATC	8 cut(s)	466, 586, 622, 655, 841, 1295, 1843, 2048
SfcI	CTRYAG	1 cut(s)	660
SfuI	TTCGAA	2 cut(s)	314, 1651
SinI	GGWCC	2 cut(s)	1312, 2087
SmiMI	CAYNNNNRTG	2 cut(s)	579, 2077
SmlI	CTYRAG	4 cut(s)	279, 324, 842, 2132
SmoI	CTYRAG	4 cut(s)	279, 324, 842, 2132
SsiI	CCGC	1 cut(s)	339
SspI	AATATT	2 cut(s)	937, 1736
SspMI	CTAG	4 cut(s)	210, 615, 1323, 2267
SstI	GAGCTC	1 cut(s)	43
StyD4I	CCNGG	6 cut(s)	114, 555, 646, 694, 1308, 2089
StyI	CCWWGG	4 cut(s)	345, 1341, 2078, 2258
TaaI	ACNGT	4 cut(s)	1213, 1373, 1874, 2062
TaiI	ACGT	1 cut(s)	1849
TaqI	TCGA	2 cut(s)	314, 1651
TatI	WGTACW	1 cut(s)	637
TfiI	GAWTC	7 cut(s)	320, 449, 515, 566, 809, 905, 1495
Tru1I	TTAA	3 cut(s)	1061, 1251, 2133
Tru9I	TTAA	3 cut(s)	1061, 1251, 2133
TscAI	CASTG	8 cut(s)	598, 754, 1222, 1378, 1540, 1726, 2170, 2329
TseFI	GTSAC	3 cut(s)	1655, 2070, 2324
TseI	GCWGC	8 cut(s)	470, 651, 654, 668, 793, 1178, 1515, 1979
Tsp45I	GTSAC	3 cut(s)	1655, 2070, 2324
TspGWI	ACGGA	2 cut(s)	1642, 2004
TspRI	CASTG	8 cut(s)	598, 754, 1222, 1378, 1540, 1726, 2170, 2329
Van91I	CCANNNNNTGG	2 cut(s)	1342, 2144
Vha464I	CTTAAG	1 cut(s)	2132
VpaK11BI	GGWCC	2 cut(s)	1312, 2087
XapI	RAATTY	3 cut(s)	1464, 1647, 1664
XceI	RCATGY	3 cut(s)	785, 1522, 1928
XcmI	CCANNNNNNNNNTGG	2 cut(s)	364, 1189
XmiI	GTMKAC	1 cut(s)	1995
XmnI	GAANNNNTTC	1 cut(s)	1686
XspI	CTAG	4 cut(s)	210, 615, 1323, 2267
Zsp2I	ATGCAT	3 cut(s)	1288, 1563, 1836

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.