Rh6AG472300

RNA cap guanine-N2 methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0014673
Physical Location & Seq
Forward (+)
65464673 .. 65466794
2122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG472300.1

Sequence Viewer

Length: 1521 bp
ATGGTTGTTGAAGCTGAAGACGAGGGGCCTGCTGTGAGAGCTCTCGGATCTCTGTTGAAGCTCACCCAAGTCTTTCTCTGGGACGATGGCACAAGAAACGACAGGTCGTTTTCCCTGGGACAGACTAAACCAGCTCAGGATGAAGGAGACGATGGCTCAAACGCTCTTTCCTCAGAATGTGGAACTTTGCCGGAGGATATGGAACTTACTAGACAGATGAATGAGTTGGGGCTTCCGGTCTCATTTCGCACAAATAAGGAGAAGCAGAAGAGAACAACTCAAGGAAAAAGAAAAGGAATGCGCCAGAAGCATTCGAACGAATCTCAAGAAGTTGTGGGCGGAGCCTTGGACTCTTCCAAAGTGAGTGTGGAGGAGATTGTATCTCCTATTATATTTGATGATAACCCAAGCAGTTCTTTGTGTTGTATGTCAATGATGGGCCAAAGTGAATCATCTTCCTCTGATGTTGCAGCTGATGCCAATAATCCAGCAAGTTCAGCTAAGATTACTGGTGATTCGCGTGATGGAATAGTCGGCATTTTTTCTAATGAAGGCCAGGATTGTGATTCCTTACACAATGGTGCCATGCTCACTGATGCAACAACAATTTTAGCTAGTTCAACTGGGTTAGATGCTGTACTTTACCCAGGCAGCTGCTCTGCAGATGCTGCTAATGGCCATGATAAGACAGAGCCAGGTGAAAGACTTATGGAGCATGACCACATAGAGTGTGCAATAATGGCTACCAGTGAAGCAGAAATAACCGAAAGTTGTGAGGAGCATGTCCCTGAGCAGCCATGTGTTTCTGAATCAGTCTCATGTTCCATATATCCAGAAGGTCTTGAGAATGATGCAATTGACAGCCAACAAAGTGGTGATTTTGGAGACTGGATGGTATATTGGGATTCTTACTACATGAGAAATTACTTCTATAATATTAGAACACATACTTCAACATGGTACCCACCCGTGGGCATGGAACATTTAGAAAATGTTGATAGCACATATAAGTCAAATGAAGGTGTTGCTGGAGTAACTCAGATGGATGTTACCACAGACTTAAAGGCAACAGATTTATGTGTTTTGAGTAAGTTTGACTCATTTGAAGAACCAATCAATGTTGATGTTTTACAATATCAACGATATGAAGAGGTTTCAAGGGATGTTGAAATTACTGCTGCCAATACTTTATTGGATACATCCACTTCAACTGTCAGTGTAAGCATATGTCCTGAGCTTCCAGATGAACTTAATCAGAGCAATAACAGGTGCAATGATGGAAATGCATCATGCTTGTCATCAAATGTCCAGGACCACATTGCTAGTTGCAGAAACCAAACCATGGAGCAGGTTGCTGATGAGGTCTGCAACAGTGATTTGCAACCAATTTTTGCAGGAAAAAGTGCTGAACCAGATATTTCTGATCTCTATGATAAACCTAATGAAGTTTACTTTTGTGAGGAAATTCCTAAATATGGTGAACATGATGAGGCGATTCAAACATTAGATACAAGCAGGTAG

Protein Analysis

506

Amino Acids

55.34

Weight (kDa)

4.22

Isoelectric Point (pI)

51.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014673)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34310 FvH4_2g34310
malus_domestica MD15G1081500.v1.1
prunus_persica Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1
pyrus_communis pycom15g07620 pycom15g07630
rosa_chinensis RchiOBHm_Chr6g0308051
rosa_laevigata RLG00000010672
rosa_multiflora Rmu_sc0002624.1_g000003
rosa_roxburghii Rroxscaffold_7G00160630
rosa_rugosa Rorug06G0363400
rosa_samantha Rh6AG472300 Rh6BG483400 Rh6DG474700
rosa_wichuraiana Rw6G041220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1339, 1506
Acc65I GGTACC 1 cut(s) 960
AccB1I GGYRCC 2 cut(s) 581, 960
AccB7I CCANNNNNTGG 1 cut(s) 1342
AccII CGCG 1 cut(s) 520
AciI CCGC 1 cut(s) 339
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 1 cut(s) 1464
AcuI CTGAAG 1 cut(s) 36
AfaI GTAC 2 cut(s) 639, 962
AfiI CCNNNNNNNGG 4 cut(s) 970, 971, 1342, 1475
AgsI TTSAA 9 cut(s) 11, 58, 621, 954, 1106, 1158, 1169, 1209, 1499
AjnI CCWGG 5 cut(s) 114, 555, 646, 694, 1308
AleI CACNNNNGTG 1 cut(s) 579
AluBI AGCT 9 cut(s) 14, 41, 61, 134, 473, 500, 614, 654, 1237
AluI AGCT 9 cut(s) 14, 41, 61, 134, 473, 500, 614, 654, 1237
Alw21I GWGCWC 1 cut(s) 43
Alw26I GTCTC 4 cut(s) 141, 244, 820, 879
AlwI GGATC 1 cut(s) 55
AlwNI CAGNNNCTG 1 cut(s) 668
AoxI GGCC 4 cut(s) 26, 439, 553, 676
ApeKI GCWGC 6 cut(s) 470, 651, 654, 668, 793, 1178
ApoI RAATTY 1 cut(s) 1464
Asp718I GGTACC 1 cut(s) 960
AspLEI GCGC 1 cut(s) 303
AspS9I GGNCC 3 cut(s) 26, 439, 1312
AsuHPI GGTGA 5 cut(s) 55, 524, 710, 887, 1490
AsuII TTCGAA 1 cut(s) 314
AvaII GGWCC 1 cut(s) 1312
BalI TGGCCA 1 cut(s) 678
BanI GGYRCC 2 cut(s) 581, 960
BanII GRGCYC 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 6 cut(s) 482, 641, 655, 663, 805, 1165
BccI CCATC 7 cut(s) 80, 146, 430, 518, 886, 1036, 1271
BcgI CGANNNNNNTGC 2 cut(s) 11, 45
BciT130I CCWGG 5 cut(s) 116, 557, 648, 696, 1310
BciVI GTATCC 1 cut(s) 1189
BcoDI GTCTC 4 cut(s) 141, 244, 820, 879
BfaI CTAG 3 cut(s) 210, 615, 1323
BfmI CTRYAG 1 cut(s) 660
BfuAI ACCTGC 2 cut(s) 1339, 1506
BfuI GTATCC 1 cut(s) 1189
BisI GCNGC 6 cut(s) 471, 652, 655, 669, 794, 1179
BlsI GCNGC 6 cut(s) 472, 653, 656, 670, 795, 1180
Bme1390I CCNGG 5 cut(s) 116, 557, 648, 696, 1310
Bme18I GGWCC 1 cut(s) 1312
BmgT120I GGNCC 3 cut(s) 26, 439, 1312
BmiI GGNNCC 4 cut(s) 27, 343, 583, 962
BmrFI CCNGG 5 cut(s) 116, 557, 648, 696, 1310
BmrI ACTGGG 1 cut(s) 633
BmsI GCATC 6 cut(s) 466, 586, 622, 655, 841, 1295
BmuI ACTGGG 1 cut(s) 633
BpiI GAAGAC 1 cut(s) 24
BplI GAGNNNNNCTC 2 cut(s) 262, 294
BpmI CTGGAG 1 cut(s) 1050
Bpu10I CCTNAGC 3 cut(s) 135, 789, 1233
Bpu14I TTCGAA 1 cut(s) 314
BpuEI CTTGAG 3 cut(s) 264, 309, 863
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 6 cut(s) 114, 115, 345, 646, 969, 1341
BsaWI WCCGGW 1 cut(s) 235
Bsc4I CCNNNNNNNGG 4 cut(s) 970, 971, 1342, 1475
Bse1I ACTGG 4 cut(s) 514, 628, 747, 893
Bse3DI GCAATG 2 cut(s) 1279, 1317
BseBI CCWGG 5 cut(s) 116, 557, 648, 696, 1310
BseDI CCNNGG 6 cut(s) 114, 115, 345, 646, 969, 1341
BseGI GGATG 5 cut(s) 145, 897, 1051, 1168, 1199
BseLI CCNNNNNNNGG 4 cut(s) 970, 971, 1342, 1475
BseMI GCAATG 2 cut(s) 1279, 1317
BseMII CTCAG 5 cut(s) 149, 186, 780, 1052, 1224
BseNI ACTGG 4 cut(s) 514, 628, 747, 893
BseRI GAGGAG 2 cut(s) 386, 791
BseXI GCAGC 6 cut(s) 482, 641, 655, 663, 805, 1165
Bsh1236I CGCG 1 cut(s) 520
BshFI GGCC 4 cut(s) 28, 441, 555, 678
BshNI GGYRCC 2 cut(s) 581, 960
BsiHKAI GWGCWC 1 cut(s) 43
BsiSI CCGG 2 cut(s) 191, 236
BslFI GGGAC 3 cut(s) 95, 132, 770
BslI CCNNNNNNNGG 4 cut(s) 970, 971, 1342, 1475
BsmAI GTCTC 4 cut(s) 141, 244, 820, 879
BsmBI CGTCTC 1 cut(s) 141
BsmFI GGGAC 3 cut(s) 95, 132, 770
BsmI GAATGC 2 cut(s) 303, 310
BsnI GGCC 4 cut(s) 28, 441, 555, 678
Bso31I GGTCTC 1 cut(s) 244
Bsp119I TTCGAA 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 2 cut(s) 47, 1423
Bsp19I CCATGG 1 cut(s) 1341
BspACI CCGC 1 cut(s) 339
BspANI GGCC 4 cut(s) 28, 441, 555, 678
BspCNI CTCAG 5 cut(s) 148, 185, 781, 1051, 1225
BspFNI CGCG 1 cut(s) 520
BspLI GGNNCC 4 cut(s) 27, 343, 583, 962
BspMAI CTGCAG 1 cut(s) 664
BspMI ACCTGC 2 cut(s) 1339, 1506
BspPI GGATC 1 cut(s) 55
BspT104I TTCGAA 1 cut(s) 314
BspT107I GGYRCC 2 cut(s) 581, 960
BspTNI GGTCTC 1 cut(s) 244
BsrDI GCAATG 2 cut(s) 1279, 1317
BsrI ACTGG 4 cut(s) 514, 628, 747, 893
BssECI CCNNGG 6 cut(s) 114, 115, 345, 646, 969, 1341
BssMI GATC 2 cut(s) 47, 1423
BssT1I CCWWGG 2 cut(s) 345, 1341
Bst2UI CCWGG 5 cut(s) 116, 557, 648, 696, 1310
Bst4CI ACNGT 2 cut(s) 1213, 1373
Bst6I CTCTTC 3 cut(s) 263, 358, 1143
BstAPI GCANNNNNTGC 2 cut(s) 476, 668
BstBI TTCGAA 1 cut(s) 314
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 6 cut(s) 135, 172, 501, 789, 1038, 1233
BstDSI CCRYGG 2 cut(s) 969, 1341
BstF5I GGATG 5 cut(s) 145, 897, 1051, 1168, 1199
BstFNI CGCG 1 cut(s) 520
BstHHI GCGC 1 cut(s) 303
BstKTI GATC 2 cut(s) 50, 1426
BstMAI GTCTC 4 cut(s) 141, 244, 820, 879
BstMBI GATC 2 cut(s) 47, 1423
BstMWI GCNNNNNNNGC 6 cut(s) 38, 307, 476, 497, 668, 740
BstNI CCWGG 5 cut(s) 116, 557, 648, 696, 1310
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 5 cut(s) 114, 555, 646, 694, 1308
BstSFI CTRYAG 1 cut(s) 660
BstUI CGCG 1 cut(s) 520
BstV1I GCAGC 6 cut(s) 482, 641, 655, 663, 805, 1165
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 47
BstXI CCANNNNNNTGG 1 cut(s) 872
BstYI RGATCY 1 cut(s) 47
BsuI GTATCC 1 cut(s) 1189
BsuRI GGCC 4 cut(s) 28, 441, 555, 678
BtgI CCRYGG 2 cut(s) 969, 1341
BtsCI GGATG 5 cut(s) 145, 897, 1051, 1168, 1199
BtsIMutI CAGTG 4 cut(s) 591, 754, 1222, 1378
BveI ACCTGC 2 cut(s) 1339, 1506
Cac8I GCNNGC 1 cut(s) 30
CaiI CAGNNNCTG 1 cut(s) 668
CfoI GCGC 1 cut(s) 303
Cfr13I GGNCC 3 cut(s) 26, 439, 1312
Csp6I GTAC 2 cut(s) 638, 961
CviQI GTAC 2 cut(s) 638, 961
DdeI CTNAG 6 cut(s) 135, 172, 501, 789, 1038, 1233
DpnI GATC 2 cut(s) 49, 1425
DpnII GATC 2 cut(s) 47, 1423
EaeI YGGCCR 1 cut(s) 676
Eam1104I CTCTTC 3 cut(s) 263, 358, 1143
EarI CTCTTC 3 cut(s) 263, 358, 1143
EciI GGCGGA 1 cut(s) 354
Ecl136II GAGCTC 1 cut(s) 41
Eco130I CCWWGG 2 cut(s) 345, 1341
Eco24I GRGCYC 1 cut(s) 43
Eco31I GGTCTC 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 1312
Eco53kI GAGCTC 1 cut(s) 41
Eco57I CTGAAG 1 cut(s) 36
EcoICRI GAGCTC 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 26
EcoRII CCWGG 5 cut(s) 114, 555, 646, 694, 1308
EcoT14I CCWWGG 2 cut(s) 345, 1341
EcoT22I ATGCAT 1 cut(s) 1288
EcoT38I GRGCYC 1 cut(s) 43
ErhI CCWWGG 2 cut(s) 345, 1341
Esp3I CGTCTC 1 cut(s) 141
FalI AAGNNNNNCTT 2 cut(s) 400, 432
FaqI GGGAC 3 cut(s) 95, 132, 770
FauNDI CATATG 1 cut(s) 1226
Fnu4HI GCNGC 6 cut(s) 471, 652, 655, 669, 794, 1179
FokI GGATG 5 cut(s) 152, 904, 1058, 1175, 1186
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 6 cut(s) 471, 652, 655, 669, 794, 1179
FspBI CTAG 3 cut(s) 210, 615, 1323
GlaI GCGC 1 cut(s) 302
GluI GCNGC 6 cut(s) 471, 652, 655, 669, 794, 1179
GsuI CTGGAG 1 cut(s) 1050
HaeIII GGCC 4 cut(s) 28, 441, 555, 678
HapII CCGG 2 cut(s) 191, 236
HhaI GCGC 1 cut(s) 303
Hin6I GCGC 1 cut(s) 301
HinP1I GCGC 1 cut(s) 301
HinfI GANTC 9 cut(s) 320, 350, 449, 515, 566, 809, 905, 1097, 1495
HpaII CCGG 2 cut(s) 191, 236
HphI GGTGA 5 cut(s) 55, 524, 710, 887, 1490
Hpy166II GTNNAC 2 cut(s) 1450, 1481
Hpy188I TCNGA 7 cut(s) 47, 175, 463, 808, 1041, 1257, 1423
Hpy188III TCNNGA 6 cut(s) 137, 326, 833, 842, 1232, 1241
Hpy8I GTNNAC 2 cut(s) 1450, 1481
HpyAV CCTTC 4 cut(s) 137, 545, 830, 1013
HpyCH4III ACNGT 2 cut(s) 1213, 1373
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 307, 476, 497, 668, 740
HpyF3I CTNAG 6 cut(s) 135, 172, 501, 789, 1038, 1233
HspAI GCGC 1 cut(s) 301
KpnI GGTACC 1 cut(s) 964
Kzo9I GATC 2 cut(s) 47, 1423
LmnI GCTCC 4 cut(s) 341, 712, 778, 1345
Lsp1109I GCAGC 6 cut(s) 482, 641, 655, 663, 805, 1165
LweI GCATC 6 cut(s) 466, 586, 622, 655, 841, 1295
MaeI CTAG 3 cut(s) 210, 615, 1323
MaeIII GTNAC 2 cut(s) 1033, 1048
MalI GATC 2 cut(s) 49, 1425
MboI GATC 2 cut(s) 47, 1423
MboII GAAGA 6 cut(s) 29, 280, 345, 447, 1118, 1160
MfeI CAATTG 1 cut(s) 855
MflI RGATCY 1 cut(s) 47
MhlI GDGCHC 1 cut(s) 43
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 6 cut(s) 606, 855, 922, 1170, 1386, 1464
MluNI TGGCCA 1 cut(s) 678
MlyI GAGTC 2 cut(s) 344, 1091
Mox20I TGGCCA 1 cut(s) 678
Mph1103I ATGCAT 1 cut(s) 1288
MscI TGGCCA 1 cut(s) 678
MseI TTAA 2 cut(s) 1061, 1251
MslI CAYNNNNRTG 1 cut(s) 579
Msp20I TGGCCA 1 cut(s) 678
MspA1I CMGCKG 2 cut(s) 473, 654
MspI CCGG 2 cut(s) 191, 236
MspR9I CCNGG 5 cut(s) 116, 557, 648, 696, 1310
MunI CAATTG 1 cut(s) 855
Mva1269I GAATGC 2 cut(s) 303, 310
MvaI CCWGG 5 cut(s) 116, 557, 648, 696, 1310
MvnI CGCG 1 cut(s) 520
MwoI GCNNNNNNNGC 6 cut(s) 38, 307, 476, 497, 668, 740
NcoI CCATGG 1 cut(s) 1341
NdeI CATATG 1 cut(s) 1226
NdeII GATC 2 cut(s) 47, 1423
NlaIV GGNNCC 4 cut(s) 27, 343, 583, 962
NsiI ATGCAT 1 cut(s) 1288
NspI RCATGY 1 cut(s) 785
NspV TTCGAA 1 cut(s) 314
OliI CACNNNNGTG 1 cut(s) 579
PasI CCCWGGG 1 cut(s) 115
PctI GAATGC 2 cut(s) 303, 310
PfeI GAWTC 7 cut(s) 320, 449, 515, 566, 809, 905, 1495
PflMI CCANNNNNTGG 1 cut(s) 1342
PfoI TCCNGGA 1 cut(s) 1308
PkrI GCNGC 6 cut(s) 472, 653, 656, 670, 795, 1180
PleI GAGTC 2 cut(s) 344, 1091
PpsI GAGTC 2 cut(s) 344, 1091
Psp124BI GAGCTC 1 cut(s) 43
Psp6I CCWGG 5 cut(s) 114, 555, 646, 694, 1308
PspGI CCWGG 5 cut(s) 114, 555, 646, 694, 1308
PspN4I GGNNCC 4 cut(s) 27, 343, 583, 962
PspPI GGNCC 3 cut(s) 26, 439, 1312
PstI CTGCAG 1 cut(s) 664
PstNI CAGNNNCTG 1 cut(s) 668
PsuI RGATCY 1 cut(s) 47
PvuII CAGCTG 2 cut(s) 473, 654
RsaI GTAC 2 cut(s) 639, 962
RsaNI GTAC 2 cut(s) 638, 961
RseI CAYNNNNRTG 1 cut(s) 579
SacI GAGCTC 1 cut(s) 43
SaqAI TTAA 2 cut(s) 1061, 1251
SatI GCNGC 6 cut(s) 471, 652, 655, 669, 794, 1179
Sau3AI GATC 2 cut(s) 47, 1423
Sau96I GGNCC 3 cut(s) 26, 439, 1312
SchI GAGTC 2 cut(s) 344, 1091
ScrFI CCNGG 5 cut(s) 116, 557, 648, 696, 1310
SduI GDGCHC 1 cut(s) 43
SfaNI GCATC 6 cut(s) 466, 586, 622, 655, 841, 1295
SfcI CTRYAG 1 cut(s) 660
SfuI TTCGAA 1 cut(s) 314
SinI GGWCC 1 cut(s) 1312
SmiMI CAYNNNNRTG 1 cut(s) 579
SmlI CTYRAG 3 cut(s) 279, 324, 842
SmoI CTYRAG 3 cut(s) 279, 324, 842
Sse9I AATT 6 cut(s) 606, 855, 922, 1170, 1386, 1464
SsiI CCGC 1 cut(s) 339
SspI AATATT 1 cut(s) 937
SspMI CTAG 3 cut(s) 210, 615, 1323
SstI GAGCTC 1 cut(s) 43
StyD4I CCNGG 5 cut(s) 114, 555, 646, 694, 1308
StyI CCWWGG 2 cut(s) 345, 1341
TaaI ACNGT 2 cut(s) 1213, 1373
TaqI TCGA 1 cut(s) 314
TasI AATT 6 cut(s) 606, 855, 922, 1170, 1386, 1464
TatI WGTACW 1 cut(s) 637
TfiI GAWTC 7 cut(s) 320, 449, 515, 566, 809, 905, 1495
Tru1I TTAA 2 cut(s) 1061, 1251
Tru9I TTAA 2 cut(s) 1061, 1251
TscAI CASTG 4 cut(s) 598, 754, 1222, 1378
TseI GCWGC 6 cut(s) 470, 651, 654, 668, 793, 1178
TspDTI ATGAA 7 cut(s) 156, 233, 564, 1032, 1161, 1260, 1458
TspRI CASTG 4 cut(s) 598, 754, 1222, 1378
Van91I CCANNNNNTGG 1 cut(s) 1342
VpaK11BI GGWCC 1 cut(s) 1312
XapI RAATTY 1 cut(s) 1464
XceI RCATGY 1 cut(s) 785
XcmI CCANNNNNNNNNTGG 2 cut(s) 364, 1189
XspI CTAG 3 cut(s) 210, 615, 1323
Zsp2I ATGCAT 1 cut(s) 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.