Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0014673

Physical Location & Seq

Genomic Coordinates Reverse (-)

3490308 .. 3494532

Gene Structure 4225 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010672

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2358 bp

ATGGTTGAAGCTGAAGACGAGGGGCCTGCTGTGAGAGCTCTCGGATCTCTGTTGAAGCTCACCCAAGTCTTTCTCTGGGACGATGGCACAAGAAACGACAGGTCGTTTTCCCTGGGACAGACTAAACCAGCTCAGGATGAAGGAGACGATGGCTCAAACGCTCTTTCCTCAGAATGTGGAACTTTGCCGGAGGATATGGAACTTACTAGACAGATGAATGAGTTGGGGCTTCCGGTCTCATTTCGCACAAATAAGGAGAAGCAGAAGAGAACAACTCAAGGAAAAAGAAAAGGAATGCGCCAGAAGCATTCGAACAAATCTCAAGAAGTTGTGGGTGGAGCCCTGGACTCTTCCAAAGTGAGTGTGGAGGAGATTGTATCTCCTATTATATTTGATGATAACCCAAGCAGTTCTTTGTGTTGTATGTCAATGATGGGCCAAAGTGAATCATCTTCCTCTGATGTTGCAGCTGATGCCAATAATCCAGCAAGTTCAGCTAAGATTACTGGTGATTCTATTAAGGAACATAAGCGTGATGGAATAGTTGGCATTGTTTCTAATGAAGGCCAGGATTGTGATTCCTTACACAATGGTGCCATGCTCACTGATGCAACAACAATTTTAGCAAGTTTAACTGGGTTAGATGCTGTACTTTACCCAGGCAGCTGCTCTGCAGATGCTGCTAGTGGCCATGATAAGACGGAGCCAGGTGAAAGACTTAAGGAGCATGACCACGTAGAGTGTGCAATAATGGCTAACAGTGAAGAAGAAATAACCGAAAGTTGTGAGGAGCATGTCCCTGAGCAGCCATGTGTTTCTGTATCAGTCTCATGTTCCATATATCCAGAAGGTCTTGAGAATGATGCAATTGACAGCCAACAAAGTGGTGATTTTGGAGACTGGATGGTATATTGGGATTCTTACTACATGAGAAATTACTTCTATAATATTAGAACACAAACTTCAACATGGTACCCACCCGTGAGCATGGAACATTTAGAAAATGTTGATATCAAATATAAGTCAAATGAAGGTGTTGCTGGAGTAACTCAGATGGATGTTACCACAGACTTAAAGGCAACAGATTTATGTGGTTTGAGTAAGTTTGACTCATTTGAAGAACCAATAAATGTTGATGTTTTACAATATCAACAATATGAAGAGGTTTCAAGGGATGTTGAAATTACTGCTGCCAATACTTTATCGGATACATCCACTTCAACTGTCAGTGTAAGCATATGTCCTGAGCTTCCAGATGAACTTAATCAGAGCAATAACAGGTGCAATGATGGAAATGCATCATACTTGTCATCAAATGTCCAGGACCACATTGCTAGTTGCAGAAACCAAACCATGGAGCAGGTTGCTGATGAGGTCTGCAACAGTGATTTGCAACCAATTTTTGTAGGAAAAAGTGCCGAACCAGATACTTCTGATCTCTATGATAAACCTAATGAAGTTTACTTTTGTGAGGAAATTCCTAAATATGGTGAACATGATGAGGCGATTCAAACATTAGATACAAGCAGCATGTCCAATTCATGCACTGAAGAAGTTGAGGATTGGAATATGCATTCTGAAACTGGAGTTCCTGCAACAAATGAGTTGGAAATGAAATCTGACCCTGCTTTAGTAAAACGGAAAAAGAAAGTGAGAAGAATTCGAAGTCACAGAAAATTCTTCAATGAGAATGAAGAAGTTCTATTTGAAGGATTGCTGAAGAAGTGTTCCACTGATATTGGTAAATATTGGTCTCAGAGATATCTATTATTCTCCAGATATGATGATGGTATAAAAATGGATGAGGAAGGATGGTTTTCAGTTACTCCAGAAGCTCTAGCTATGCATCATGCCCAACGTTGTGGTAGTGGCATTATCATTGACTGTTTTACTGGAGTTGGTGGTAATGCCATCCAATTTGCTCAGATAAGCAAACATGTAACTGCCATTGATATTGATCCAACAAAGATTGATTATGCTCAACATAATGCTGCTATCTACGGAGTAGACGAAAGGATAGATTTCATAAAGGGGGACTTTTTCCATTTGGCATCAAGGTTGAAGGCAGATACAGTCTTTTTGTCACCACCATGGGGAGGACCTGGTTATGCAAGAGAAGAGACATATGACATTATGACAATGCTTAAGCCACATGATGGATATTTTCTCTTCAACACTGCTAAGCAAGTTGCTTCCAAGATAGTCATGTTTCTCCCAAGAAATGTTGATGTCAACCAATTAGCTGAGTTGTCTCTTTCAGGGACTCCACCATGGTCGCTAGAGGTGGAGAAAAACTTTGTAAATGGCAAGTTGAAGGGAATAACTGCTTACTTCAGTGACACGGCAAGGAGAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016741 GO:0032259 GO:0034641 GO:0036260 GO:0036261 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071164 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Methyltransf_15 WW

Protein Analysis

786

Amino Acids

87.07

Weight (kDa)

4.62

Isoelectric Point (pI)

42.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_15	PF09445	625 - 779	3.7e-43	RNA cap guanine-N2 methyltransferase
TRM5-TYW2_MTfase	PF02475	625 - 695	2.9e-07	TRM5/TYW2 methyltransferase domain
Methyltransf_31	PF13847	626 - 728	2.7e-07	Methyltransferase domain
Methyltransf_25	PF13649	626 - 693	7.5e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014673)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34310 FvH4_2g34310
malus_domestica	MD15G1081500.v1.1
prunus_persica	Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1 Prupe.1G434300_v2.0.a1
pyrus_communis	pycom15g07620 pycom15g07630
rosa_chinensis	RchiOBHm_Chr6g0308051
rosa_laevigata	RLG00000010672
rosa_multiflora	Rmu_sc0002624.1_g000003
rosa_roxburghii	Rroxscaffold_7G00160630
rosa_rugosa	Rorug06G0363400
rosa_samantha	Rh6AG472300 Rh6BG483400 Rh6DG474700
rosa_wichuraiana	Rw6G041220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1351
Acc65I	GGTACC	1 cut(s)	972
AccB1I	GGYRCC	2 cut(s)	593, 972
AccB7I	CCANNNNNTGG	2 cut(s)	1354, 2156
AccI	GTMKAC	1 cut(s)	2007
AclI	AACGTT	1 cut(s)	1858
AclWI	GGATC	2 cut(s)	52, 1952
AcoI	YGGCCR	1 cut(s)	688
AcsI	RAATTY	3 cut(s)	1476, 1659, 1676
AcuI	CTGAAG	4 cut(s)	33, 1569, 1739, 2317
AfaI	GTAC	2 cut(s)	651, 974
AfiI	CCNNNNNNNGG	5 cut(s)	1354, 1487, 2093, 2096, 2156
AflII	CTTAAG	2 cut(s)	719, 2144
AflIII	ACRYGT	1 cut(s)	1936
AjnI	CCWGG	7 cut(s)	111, 342, 567, 658, 706, 1320, 2101
AleI	CACNNNNGTG	1 cut(s)	591
Alw21I	GWGCWC	1 cut(s)	40
Alw26I	GTCTC	7 cut(s)	138, 241, 832, 891, 1758, 2114, 2256
AlwI	GGATC	2 cut(s)	52, 1952
AlwNI	CAGNNNCTG	1 cut(s)	680
AoxI	GGCC	4 cut(s)	23, 436, 565, 688
ApeKI	GCWGC	8 cut(s)	467, 663, 666, 680, 805, 1190, 1527, 1991
ApoI	RAATTY	3 cut(s)	1476, 1659, 1676
Asp700I	GAANNNNTTC	1 cut(s)	1698
Asp718I	GGTACC	1 cut(s)	972
AspLEI	GCGC	1 cut(s)	300
AspS9I	GGNCC	4 cut(s)	23, 436, 1324, 2099
AsuHPI	GGTGA	6 cut(s)	52, 521, 722, 899, 1502, 2076
AsuII	TTCGAA	2 cut(s)	311, 1663
AvaII	GGWCC	2 cut(s)	1324, 2099
BalI	TGGCCA	1 cut(s)	690
BanI	GGYRCC	2 cut(s)	593, 972
BanII	GRGCYC	2 cut(s)	40, 343
BbsI	GAAGAC	1 cut(s)	21
Bbv12I	GWGCWC	1 cut(s)	40
BbvI	GCAGC	8 cut(s)	479, 653, 667, 675, 817, 1177, 1539, 1978
BcgI	CGANNNNNNTGC	2 cut(s)	8, 42
BciT130I	CCWGG	7 cut(s)	113, 344, 569, 660, 708, 1322, 2103
BciVI	GTATCC	1 cut(s)	1201
BcoDI	GTCTC	7 cut(s)	138, 241, 832, 891, 1758, 2114, 2256
BfaI	CTAG	5 cut(s)	207, 684, 1335, 1838, 2279
BfmI	CTRYAG	1 cut(s)	672
BfrI	CTTAAG	2 cut(s)	719, 2144
BfuAI	ACCTGC	1 cut(s)	1351
BfuI	GTATCC	1 cut(s)	1201
BisI	GCNGC	8 cut(s)	468, 664, 667, 681, 806, 1191, 1528, 1992
BlpI	GCTNAGC	1 cut(s)	2181
BlsI	GCNGC	8 cut(s)	469, 665, 668, 682, 807, 1192, 1529, 1993
Bme1390I	CCNGG	7 cut(s)	113, 344, 569, 660, 708, 1322, 2103
Bme18I	GGWCC	2 cut(s)	1324, 2099
BmgT120I	GGNCC	4 cut(s)	23, 436, 1324, 2099
BmiI	GGNNCC	5 cut(s)	24, 340, 595, 705, 974
BmrFI	CCNGG	7 cut(s)	113, 344, 569, 660, 708, 1322, 2103
BmrI	ACTGGG	1 cut(s)	645
BmsI	GCATC	8 cut(s)	463, 598, 634, 667, 853, 1307, 1855, 2060
BmuI	ACTGGG	1 cut(s)	645
BpiI	GAAGAC	1 cut(s)	21
BplI	GAGNNNNNCTC	2 cut(s)	259, 291
BpmI	CTGGAG	5 cut(s)	1062, 1605, 1759, 1812, 1914
Bpu10I	CCTNAGC	3 cut(s)	132, 801, 1245
Bpu1102I	GCTNAGC	1 cut(s)	2181
Bpu14I	TTCGAA	2 cut(s)	311, 1663
BpuEI	CTTGAG	3 cut(s)	261, 306, 875
BsaAI	YACGTR	1 cut(s)	736
BsaBI	GATNNNNATC	1 cut(s)	1956
BsaI	GGTCTC	2 cut(s)	241, 1758
BsaJI	CCNNGG	7 cut(s)	111, 112, 342, 658, 1353, 2090, 2270
BsaWI	WCCGGW	1 cut(s)	232
Bsc4I	CCNNNNNNNGG	5 cut(s)	1354, 1487, 2093, 2096, 2156
Bse1I	ACTGG	5 cut(s)	511, 640, 905, 1588, 1897
Bse3DI	GCAATG	2 cut(s)	1291, 1329
Bse8I	GATNNNNATC	1 cut(s)	1956
BseBI	CCWGG	7 cut(s)	113, 344, 569, 660, 708, 1322, 2103
BseDI	CCNNGG	7 cut(s)	111, 112, 342, 658, 1353, 2090, 2270
BseGI	GGATG	8 cut(s)	142, 909, 1063, 1180, 1211, 1807, 1817, 1911
BseJI	GATNNNNATC	1 cut(s)	1956
BseLI	CCNNNNNNNGG	5 cut(s)	1354, 1487, 2093, 2096, 2156
BseMI	GCAATG	2 cut(s)	1291, 1329
BseMII	CTCAG	8 cut(s)	146, 183, 792, 1064, 1236, 1769, 1937, 2235
BseNI	ACTGG	5 cut(s)	511, 640, 905, 1588, 1897
BseRI	GAGGAG	2 cut(s)	383, 803
BseXI	GCAGC	8 cut(s)	479, 653, 667, 675, 817, 1177, 1539, 1978
BshFI	GGCC	4 cut(s)	25, 438, 567, 690
BshNI	GGYRCC	2 cut(s)	593, 972
BsiHKAI	GWGCWC	1 cut(s)	40
BsiSI	CCGG	2 cut(s)	188, 233
BslFI	GGGAC	5 cut(s)	92, 129, 782, 2048, 2275
BslI	CCNNNNNNNGG	5 cut(s)	1354, 1487, 2093, 2096, 2156
BsmAI	GTCTC	7 cut(s)	138, 241, 832, 891, 1758, 2114, 2256
BsmBI	CGTCTC	1 cut(s)	138
BsmFI	GGGAC	5 cut(s)	92, 129, 782, 2048, 2275
BsmI	GAATGC	3 cut(s)	300, 307, 1573
BsnI	GGCC	4 cut(s)	25, 438, 567, 690
Bso31I	GGTCTC	2 cut(s)	241, 1758
Bsp119I	TTCGAA	2 cut(s)	311, 1663
Bsp1286I	GDGCHC	2 cut(s)	40, 343
Bsp143I	GATC	3 cut(s)	44, 1435, 1957
Bsp1720I	GCTNAGC	1 cut(s)	2181
Bsp19I	CCATGG	3 cut(s)	1353, 2090, 2270
BspANI	GGCC	4 cut(s)	25, 438, 567, 690
BspCNI	CTCAG	8 cut(s)	145, 182, 793, 1063, 1237, 1768, 1936, 2236
BspLI	GGNNCC	5 cut(s)	24, 340, 595, 705, 974
BspMAI	CTGCAG	1 cut(s)	676
BspMI	ACCTGC	1 cut(s)	1351
BspPI	GGATC	2 cut(s)	52, 1952
BspT104I	TTCGAA	2 cut(s)	311, 1663
BspT107I	GGYRCC	2 cut(s)	593, 972
BspTI	CTTAAG	2 cut(s)	719, 2144
BspTNI	GGTCTC	2 cut(s)	241, 1758
BsrDI	GCAATG	2 cut(s)	1291, 1329
BsrI	ACTGG	5 cut(s)	511, 640, 905, 1588, 1897
BssECI	CCNNGG	7 cut(s)	111, 112, 342, 658, 1353, 2090, 2270
BssMI	GATC	3 cut(s)	44, 1435, 1957
BssT1I	CCWWGG	3 cut(s)	1353, 2090, 2270
Bst2UI	CCWGG	7 cut(s)	113, 344, 569, 660, 708, 1322, 2103
Bst4CI	ACNGT	5 cut(s)	761, 1225, 1385, 1886, 2074
Bst6I	CTCTTC	5 cut(s)	260, 355, 1155, 2112, 2174
BstAFI	CTTAAG	2 cut(s)	719, 2144
BstAPI	GCANNNNNTGC	2 cut(s)	473, 680
BstBAI	YACGTR	1 cut(s)	736
BstBI	TTCGAA	2 cut(s)	311, 1663
BstC8I	GCNNGC	1 cut(s)	27
BstDSI	CCRYGG	3 cut(s)	1353, 2090, 2270
BstF5I	GGATG	8 cut(s)	142, 909, 1063, 1180, 1211, 1807, 1817, 1911
BstHHI	GCGC	1 cut(s)	300
BstKTI	GATC	3 cut(s)	47, 1438, 1960
BstMAI	GTCTC	7 cut(s)	138, 241, 832, 891, 1758, 2114, 2256
BstMBI	GATC	3 cut(s)	44, 1435, 1957
BstMWI	GCNNNNNNNGC	6 cut(s)	35, 304, 473, 494, 680, 752
BstNI	CCWGG	7 cut(s)	113, 344, 569, 660, 708, 1322, 2103
BstNSI	RCATGY	3 cut(s)	797, 1534, 1940
BstSCI	CCNGG	7 cut(s)	111, 342, 567, 658, 706, 1320, 2101
BstSFI	CTRYAG	1 cut(s)	672
BstV1I	GCAGC	8 cut(s)	479, 653, 667, 675, 817, 1177, 1539, 1978
BstV2I	GAAGAC	1 cut(s)	21
BstX2I	RGATCY	1 cut(s)	44
BstXI	CCANNNNNNTGG	2 cut(s)	884, 1862
BstYI	RGATCY	1 cut(s)	44
BsuI	GTATCC	1 cut(s)	1201
BsuRI	GGCC	4 cut(s)	25, 438, 567, 690
BtgI	CCRYGG	3 cut(s)	1353, 2090, 2270
BtsCI	GGATG	8 cut(s)	142, 909, 1063, 1180, 1211, 1807, 1817, 1911
BtsI	GCAGTG	1 cut(s)	2175
BtsIMutI	CAGTG	8 cut(s)	603, 766, 1234, 1390, 1545, 1731, 2175, 2341
BveI	ACCTGC	1 cut(s)	1351
Cac8I	GCNNGC	1 cut(s)	27
CaiI	CAGNNNCTG	1 cut(s)	680
CfoI	GCGC	1 cut(s)	300
Cfr13I	GGNCC	4 cut(s)	23, 436, 1324, 2099
CsiI	ACCWGGT	1 cut(s)	2101
Csp6I	GTAC	2 cut(s)	650, 973
CspCI	CAANNNNNGTGG	2 cut(s)	1720, 1755
CviQI	GTAC	2 cut(s)	650, 973
DpnI	GATC	3 cut(s)	46, 1437, 1959
DpnII	GATC	3 cut(s)	44, 1435, 1957
EaeI	YGGCCR	1 cut(s)	688
Eam1104I	CTCTTC	5 cut(s)	260, 355, 1155, 2112, 2174
EarI	CTCTTC	5 cut(s)	260, 355, 1155, 2112, 2174
Ecl136II	GAGCTC	1 cut(s)	38
Eco130I	CCWWGG	3 cut(s)	1353, 2090, 2270
Eco24I	GRGCYC	2 cut(s)	40, 343
Eco31I	GGTCTC	2 cut(s)	241, 1758
Eco32I	GATATC	2 cut(s)	1012, 1763
Eco47I	GGWCC	2 cut(s)	1324, 2099
Eco53kI	GAGCTC	1 cut(s)	38
Eco57I	CTGAAG	4 cut(s)	33, 1569, 1739, 2317
EcoICRI	GAGCTC	1 cut(s)	38
EcoO109I	RGGNCCY	2 cut(s)	23, 2099
EcoRI	GAATTC	1 cut(s)	1659
EcoRII	CCWGG	7 cut(s)	111, 342, 567, 658, 706, 1320, 2101
EcoRV	GATATC	2 cut(s)	1012, 1763
EcoT14I	CCWWGG	3 cut(s)	1353, 2090, 2270
EcoT22I	ATGCAT	3 cut(s)	1300, 1575, 1848
EcoT38I	GRGCYC	2 cut(s)	40, 343
ErhI	CCWWGG	3 cut(s)	1353, 2090, 2270
Esp3I	CGTCTC	1 cut(s)	138
FalI	AAGNNNNNCTT	4 cut(s)	397, 429, 2021, 2053
FaqI	GGGAC	5 cut(s)	92, 129, 782, 2048, 2275
FauNDI	CATATG	2 cut(s)	1238, 2125
FblI	GTMKAC	1 cut(s)	2007
Fnu4HI	GCNGC	8 cut(s)	468, 664, 667, 681, 806, 1191, 1528, 1992
FokI	GGATG	8 cut(s)	149, 916, 1070, 1187, 1198, 1814, 1824, 1898
FriOI	GRGCYC	2 cut(s)	40, 343
Fsp4HI	GCNGC	8 cut(s)	468, 664, 667, 681, 806, 1191, 1528, 1992
FspBI	CTAG	5 cut(s)	207, 684, 1335, 1838, 2279
GlaI	GCGC	1 cut(s)	299
GluI	GCNGC	8 cut(s)	468, 664, 667, 681, 806, 1191, 1528, 1992
GsuI	CTGGAG	5 cut(s)	1062, 1605, 1759, 1812, 1914
HaeIII	GGCC	4 cut(s)	25, 438, 567, 690
HapII	CCGG	2 cut(s)	188, 233
HhaI	GCGC	1 cut(s)	300
Hin6I	GCGC	1 cut(s)	298
HinP1I	GCGC	1 cut(s)	298
HincII	GTYRAC	1 cut(s)	2233
HindII	GTYRAC	1 cut(s)	2233
HinfI	GANTC	8 cut(s)	347, 446, 512, 578, 917, 1109, 1507, 2263
HpaII	CCGG	2 cut(s)	188, 233
HphI	GGTGA	6 cut(s)	52, 521, 722, 899, 1502, 2076
Hpy166II	GTNNAC	4 cut(s)	1462, 1493, 2008, 2233
Hpy188III	TCNNGA	8 cut(s)	134, 323, 845, 854, 1244, 1253, 1776, 1829
Hpy8I	GTNNAC	4 cut(s)	1462, 1493, 2008, 2233
HpyAV	CCTTC	8 cut(s)	134, 557, 842, 1025, 1703, 1802, 2056, 2308
HpyCH4III	ACNGT	5 cut(s)	761, 1225, 1385, 1886, 2074
HpyCH4IV	ACGT	2 cut(s)	735, 1858
HpyF10VI	GCNNNNNNNGC	6 cut(s)	35, 304, 473, 494, 680, 752
HpySE526I	ACGT	2 cut(s)	735, 1858
HspAI	GCGC	1 cut(s)	298
KpnI	GGTACC	1 cut(s)	976
Kzo9I	GATC	3 cut(s)	44, 1435, 1957
LmnI	GCTCC	5 cut(s)	338, 703, 724, 790, 1357
Lsp1109I	GCAGC	8 cut(s)	479, 653, 667, 675, 817, 1177, 1539, 1978
LweI	GCATC	8 cut(s)	463, 598, 634, 667, 853, 1307, 1855, 2060
MabI	ACCWGGT	1 cut(s)	2101
MaeI	CTAG	5 cut(s)	207, 684, 1335, 1838, 2279
MaeII	ACGT	2 cut(s)	735, 1858
MaeIII	GTNAC	7 cut(s)	1045, 1060, 1667, 1822, 1939, 2082, 2336
MalI	GATC	3 cut(s)	46, 1437, 1959
MboI	GATC	3 cut(s)	44, 1435, 1957
MfeI	CAATTG	1 cut(s)	867
MflI	RGATCY	1 cut(s)	44
MhlI	GDGCHC	2 cut(s)	40, 343
MlsI	TGGCCA	1 cut(s)	690
MluNI	TGGCCA	1 cut(s)	690
MlyI	GAGTC	3 cut(s)	341, 1103, 2257
MmeI	TCCRAC	2 cut(s)	1587, 1985
Mox20I	TGGCCA	1 cut(s)	690
Mph1103I	ATGCAT	3 cut(s)	1300, 1575, 1848
MroXI	GAANNNNTTC	1 cut(s)	1698
MscI	TGGCCA	1 cut(s)	690
MseI	TTAA	6 cut(s)	519, 632, 720, 1073, 1263, 2145
MslI	CAYNNNNRTG	3 cut(s)	531, 591, 2089
Msp20I	TGGCCA	1 cut(s)	690
MspA1I	CMGCKG	2 cut(s)	470, 666
MspCI	CTTAAG	2 cut(s)	719, 2144
MspI	CCGG	2 cut(s)	188, 233
MspR9I	CCNGG	7 cut(s)	113, 344, 569, 660, 708, 1322, 2103
MunI	CAATTG	1 cut(s)	867
Mva1269I	GAATGC	3 cut(s)	300, 307, 1573
MvaI	CCWGG	7 cut(s)	113, 344, 569, 660, 708, 1322, 2103
MwoI	GCNNNNNNNGC	6 cut(s)	35, 304, 473, 494, 680, 752
NcoI	CCATGG	3 cut(s)	1353, 2090, 2270
NdeI	CATATG	2 cut(s)	1238, 2125
NdeII	GATC	3 cut(s)	44, 1435, 1957
NlaIV	GGNNCC	5 cut(s)	24, 340, 595, 705, 974
NmuCI	GTSAC	3 cut(s)	1667, 2082, 2336
NsiI	ATGCAT	3 cut(s)	1300, 1575, 1848
NspI	RCATGY	3 cut(s)	797, 1534, 1940
NspV	TTCGAA	2 cut(s)	311, 1663
OliI	CACNNNNGTG	1 cut(s)	591
PasI	CCCWGGG	1 cut(s)	112
PciI	ACATGT	1 cut(s)	1936
PcsI	WCGNNNNNNNCGW	1 cut(s)	2007
PctI	GAATGC	3 cut(s)	300, 307, 1573
PdmI	GAANNNNTTC	1 cut(s)	1698
PfeI	GAWTC	5 cut(s)	446, 512, 578, 917, 1507
PflMI	CCANNNNNTGG	2 cut(s)	1354, 2156
PfoI	TCCNGGA	1 cut(s)	1320
PkrI	GCNGC	8 cut(s)	469, 665, 668, 682, 807, 1192, 1529, 1993
PleI	GAGTC	3 cut(s)	341, 1103, 2257
PpsI	GAGTC	3 cut(s)	341, 1103, 2257
Ppu21I	YACGTR	1 cut(s)	736
PpuMI	RGGWCCY	1 cut(s)	2099
PscI	ACATGT	1 cut(s)	1936
Psp124BI	GAGCTC	1 cut(s)	40
Psp1406I	AACGTT	1 cut(s)	1858
Psp5II	RGGWCCY	1 cut(s)	2099
Psp6I	CCWGG	7 cut(s)	111, 342, 567, 658, 706, 1320, 2101
PspGI	CCWGG	7 cut(s)	111, 342, 567, 658, 706, 1320, 2101
PspN4I	GGNNCC	5 cut(s)	24, 340, 595, 705, 974
PspPI	GGNCC	4 cut(s)	23, 436, 1324, 2099
PspPPI	RGGWCCY	1 cut(s)	2099
PstI	CTGCAG	1 cut(s)	676
PstNI	CAGNNNCTG	1 cut(s)	680
PsuI	RGATCY	1 cut(s)	44
PvuII	CAGCTG	2 cut(s)	470, 666
RsaI	GTAC	2 cut(s)	651, 974
RsaNI	GTAC	2 cut(s)	650, 973
RseI	CAYNNNNRTG	3 cut(s)	531, 591, 2089
SacI	GAGCTC	1 cut(s)	40
SaqAI	TTAA	6 cut(s)	519, 632, 720, 1073, 1263, 2145
SatI	GCNGC	8 cut(s)	468, 664, 667, 681, 806, 1191, 1528, 1992
Sau3AI	GATC	3 cut(s)	44, 1435, 1957
Sau96I	GGNCC	4 cut(s)	23, 436, 1324, 2099
SchI	GAGTC	3 cut(s)	341, 1103, 2257
ScrFI	CCNGG	7 cut(s)	113, 344, 569, 660, 708, 1322, 2103
SduI	GDGCHC	2 cut(s)	40, 343
SexAI	ACCWGGT	1 cut(s)	2101
SfaNI	GCATC	8 cut(s)	463, 598, 634, 667, 853, 1307, 1855, 2060
SfcI	CTRYAG	1 cut(s)	672
SfuI	TTCGAA	2 cut(s)	311, 1663
SinI	GGWCC	2 cut(s)	1324, 2099
SmiMI	CAYNNNNRTG	3 cut(s)	531, 591, 2089
SmlI	CTYRAG	5 cut(s)	276, 321, 719, 854, 2144
SmoI	CTYRAG	5 cut(s)	276, 321, 719, 854, 2144
SspI	AATATT	2 cut(s)	949, 1748
SspMI	CTAG	5 cut(s)	207, 684, 1335, 1838, 2279
SstI	GAGCTC	1 cut(s)	40
StyD4I	CCNGG	7 cut(s)	111, 342, 567, 658, 706, 1320, 2101
StyI	CCWWGG	3 cut(s)	1353, 2090, 2270
TaaI	ACNGT	5 cut(s)	761, 1225, 1385, 1886, 2074
TaiI	ACGT	2 cut(s)	738, 1861
TaqI	TCGA	2 cut(s)	311, 1663
TatI	WGTACW	1 cut(s)	649
TfiI	GAWTC	5 cut(s)	446, 512, 578, 917, 1507
Tru1I	TTAA	6 cut(s)	519, 632, 720, 1073, 1263, 2145
Tru9I	TTAA	6 cut(s)	519, 632, 720, 1073, 1263, 2145
TscAI	CASTG	8 cut(s)	610, 766, 1234, 1390, 1552, 1738, 2182, 2341
TseFI	GTSAC	3 cut(s)	1667, 2082, 2336
TseI	GCWGC	8 cut(s)	467, 663, 666, 680, 805, 1190, 1527, 1991
Tsp45I	GTSAC	3 cut(s)	1667, 2082, 2336
TspGWI	ACGGA	3 cut(s)	716, 1654, 2016
TspRI	CASTG	8 cut(s)	610, 766, 1234, 1390, 1552, 1738, 2182, 2341
Van91I	CCANNNNNTGG	2 cut(s)	1354, 2156
Vha464I	CTTAAG	2 cut(s)	719, 2144
VpaK11BI	GGWCC	2 cut(s)	1324, 2099
XapI	RAATTY	3 cut(s)	1476, 1659, 1676
XceI	RCATGY	3 cut(s)	797, 1534, 1940
XcmI	CCANNNNNNNNNTGG	1 cut(s)	361
XmiI	GTMKAC	1 cut(s)	2007
XmnI	GAANNNNTTC	1 cut(s)	1698
XspI	CTAG	5 cut(s)	207, 684, 1335, 1838, 2279
Zsp2I	ATGCAT	3 cut(s)	1300, 1575, 1848

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.